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| 1 | A seven-gene-deleted African swine fever virus is safe and effective as a live attenuated vaccine in pigs显示文摘African swine fever(ASF)is a devastating infectious disease in swine that is severely threatening the global pig industry.An efficacious vaccine is urgently required.Here,we used the Chinese ASFV HLJ/18 as a backbone and generated a series of genedeleted viruses.The virulence,immunogenicity,safety,and protective efficacy evaluation in specific-pathogen-free pigs,commercial pigs,and pregnant sows indicated that one virus,namely HLJ/18-7GD,which has seven genes deleted,is fully attenuated in pigs,cannot convert to the virulent strain,and provides complete protection of pigs against lethal ASFV challenge.Our study shows that HLJ/-18-7GD is a safe and effective vaccine against ASFV,and as such is expected to play an important role in controlling the spread of ASFV. | Weiye Chen Dongming Zhao Xijun He Renqiang Liu Zilong Wang Xianfeng Zhang Fang Li Dan Shan Hefeng Chen Jiwen Zhang Lulu Wang Zhiyuan Wen Xijun Wang Yuntao Guan Jinxiong Liu Zhigao Bu | 2020 | Science China(Life Sciences)2020，63，5： | 72 |
| 2 | Emergence and prevalence of naturally occurring lower virulent African swine fever viruses in domestic pigs in China in 2020显示文摘African swine fever virus(ASFV)has been circulating in China for more than two years,and it is not clear whether the biological properties of the virus have changed.Here,we report on our surveillance of ASFVs in seven provinces of China,from June to December,2020.A total of 22 viruses were isolated and characterized as genotype II ASFVs,with mutations,deletions,insertions,or short-fragment replacement occurring in all isolates compared with Pig/HLJ/2018(HLJ/18),the earliest isolate in China.Eleven isolates had four different types of natural mutations or deletion in the EP402R gene and displayed a nonhemadsorbing(non-HAD)phenotype.Four isolates were tested for virulence in pigs;two were found to be as highly lethal as HLJ/18.However,two non-HAD isolates showed lower virulence but were highly transmissible;infection with 106 TCID50 dose was partially lethal and caused acute or sub-acute disease,whereas 103 TCID50 dose caused non-lethal,sub-acute or chronic disease,and persistent infection.The emergence of lower virulent natural mutants brings greater difficulty to the early diagnosis of ASF and creates new challenges for ASFV control. | Encheng Sun Zhenjiang Zhang Zilong Wang Xijun He Xianfeng Zhang Lulu Wang Wenqing Wang Lianyu Huang Fei Xi Haoyue Huangfu Ghebremedhin Tsegay Hong Huo Jianhong Sun Zhijun Tian Wei Xia Xuewu Yu Fang Li Renqiang Liu Yuntao Guan Dongming Zhao Zhigao Bu | 2021 | Science China(Life Sciences)2021，64，5： | 46 |
| 3 | Study on the structure characterization and moisturizing effect of Tremella polysaccharide fermented from GCMCC5.39显示文摘The structure and moisture retention of Tremella polysaccharide fermented from GCMCC5.39(FTP)were evaluated.After UV,infrared spectrum analysis,HPAEC-PAD,HPSEC and 1 D NMR analysis,the composition of the purifi ed FTP was determined.Purifi ed components of fermented Tremella polysaccharide(FTPS)was made of galactose,mannose,glucose,galactosmine,glucosamine,and contain a large amount of hydroxyl,carbonyl and amino groups.FTPS wasα-neutral pyranose without uronic acid.FTPS-1 and FTPS-2 were obtained after purifi cation by DEAE-Sepharose Fast Flow Column.The molecular weights of FTPS-1 and FTPS-2 were 25722 and 177263 Da.FTPS-2 had a better ability to prevent moisture loss,and the optimal moisture retention period was 0–4 h.FTPS-2 could signifi cantly increase the moisture content of the skin epidermis and showed a dose-concentration relationship.The effect of FTPS-2 on the expression of different moisturizing genes was evaluated in a human skin keratinocyte model.The results showed that FTPS-2 has no cytotoxicity,and could signifi cantly promote AQP3,TGM1,CASP14,HYAL2,FLG gene expression level in HaCaT cells.It has the most signifi cant infl uence at HYAL2 protein expression on 50μg/mL. | Meng Yang Zilong Zhang Yan He Chengliang Li Jinmei Wang Xia Ma | 2021 | Food Science and Human Wellness2021，10，4： | 6 |
| 4 | The Research on Social Networks Public Opinion Propagation Influence Models and Its Controllability显示文摘Public opinion propagation control is one of the hot topics in contemporary social network research. With the rapid dissemination of information over the Internet, the traditional isolation and vaccination strategies can no longer achieve satisfactory results. A positive guidance technology for public opinion diffusion is urgently needed. First, based on the analysis of influence network controllability and public opinion diffusion, a positive guidance technology is proposed and a new model that supports external control is established. Second, in combination with the influence network, a public opinion propagation influence network model is designed and a public opinion control point selection algorithm(POCDNSA) is proposed. Finally, An experiment verified that this algorithm can lead to users receiving the correct guidance quickly and accurately, reducing the impact of false public opinion information; the effect of CELF is no better than that of the POCDNSA algorithm. The main reason is that the former is completely based on the diffusion cascade information contained in the training data, but does not consider the specific situation of the network structure and the diffusion of public opinion information in the closed set. thus, the effectiveness and feasibility of the algorithm is proven. The findings of this article therefore provide useful insights for the implementation of public opinion control. | Lejun Zhang Tong Wang Zilong Jin Nan Su Chunhui Zhao Yongjun He | 2018 | China Communications2018，15，7： | 6 |
| 5 | Cutting performance of micro-textured PCBN tool显示文摘To study the efect of micro-texture on the cutting performance of polyrystalline cubic boron nitide(PCBN)tools,five types of micro-textures(circular pits,eliptical grooves,transverse grooves,composite grooves,and wavy grooves)were applied to the rake surface of PCBN tools by an optical fber laser marking machine.Through a combination of three dimensional cutting simulations and experiments,the influences of micro-texture on chip-tool contact area,cutting force,chip morphology,shear angle,and surface roughness during the cuting process were analyzed.The results indicated that the chip--tool contact area and cutting force of both non-textured and micro textured tools increased with increasing cutting speed,while the shear angle decreased with increasing cutting speed.The chip-tool contact area and cutting force of the five types of micro-textured tools were smaller than those of the non textured tool The chip-tool contact area and cutting force obtained by the wavy-groove micro textured tool were the smallest.The chip radius produced by the five types of micro-textured tools was smaller than that produced by the non-textured tool,and the chip morphology was more stable.The transverse-groove micro-textured tool had a better chip breaking efect.The chip rnadius generated by the lliptical groove micro textured tool was 0.96 cm,while that generated by the wavy-groove tool varied from 0.55 cm to 1.26 cm.The presence of a micro-texture reduced the surface roughness of the workpiece by 11.73%-56.7%.Under the same cutting conditions,the five types of micro-textured tools gave a smaller chip--tool contact area,cutting force,chip radius,and surface roughness and a larger shear angle than the non-textured tool.In addition,the elliptical groove and wavy-groove micro-textured tools had better cuting performance. | Lin Fan Zilong Deng Xingjun Gao Yan He | 2021 | Nanotechnology and Precision Engineering2021，4，2： | 3 |
| 6 | Characteristics of Uranium Mineralization in Red Clastic Formations in the Southwestern Margin of the Ordos Basin显示文摘In the southwestern margin of the Ordos Basin,uranium mineralization is primarily hosted by predominantly oxidative red clastic formations in the Lower Cretaceous.The main target layers for uranium exploration are the Madongshan and Liwaxia formations of the Liupanshan Group,followed by the Jingchuan Formation of the Zhidan Group.The host rocks(medium-fine feldspar quartz sandstone),which are bleached to a light grayish white color,contain a minor organic matter component and pyrite.Uranium mineralization changes from surficial infiltration or phreatic oxidation in the upper part to interlayer oxidation in the lower part.Uranium ore bodies are mostly lenticular or tabular in shape,locally shaped like crescent rolls.Individual ore bodies are typically small and shallow.Uranium predominantly manifests as pitchblende and coffinite.Coffinite is usually short and columnar or granular in habit,whereas pitchblende occurs as an irregular colloidal covering on the surface or in fissures of ferric oxide,silicate,clay or carbonate.Secondary uranium minerals are torbernite,uranophane,and uranopilite.Minerals associated with uranium are mainly pyrite,chalcopyrite and,to a minor extent,arsenopyrite and fluorite.The associated elements are Mo,V,Se,Co,Ni,and Mn,the host sandstone being high in Cu and Ba.Overall,the red clastic formations in the southwestern margin of the Ordos Basin are characterized by’five multiples but one low’which means multiple target layers,multiple stages of mineralization,multiple ore body shapes,multiple kinds of uranium minerals,multiple associated elements,but low organic matter.This implies an overall complex uranium metallogenic environment and mineralization process.It is recommended that future uranium exploration should take into consideration regional metallogenic conditions and mineralization features,with target layers in the wide-smooth synclinal slope being focused on.Most uranium deposits are small to medium in size,and the main type of uranium mineralization can vary by target layer. | ZHANG Zilong HE Feng YI Longsheng FAN Honghai CAI Yuqi LIU Hongxu LIU Xinyang CHEN Hongbin JIA Cui YANG Mengjia | 2020 | Acta Geologica Sinica(English Edition)2020，94，5： | 1 |
| 7 | An Intronic Variant of CHD7 Identified in Autism Patients Interferes with Neuronal Differentiation and Development显示文摘Genetic composition plays critical roles in the pathogenesis of autism spectrum disorder(ASD).Especially,inherited and de novo intronic variants are often seen in patients with ASD.However,the biological significance of intronic variants is difficult to address.Here,among a Chinese ASD cohort,we identified a recurrent inherited intronic variant in the CHD7 gene,which is specifically enriched in East Asian populations.CHD7 has been implicated in numerous developmental disorders including CHARGE syndrome and ASD.To investigate whether the ASD-associated CHD7 intronic variant affects neural development,we established human embryonic stem cells carrying this variant using CRISPR/Cas9 methods and found that the level of CHD7 mRNA significantly decreased compared to control.Upon differentiation towards the forebrain neuronal lineage,we found that neural cells carrying the CHD7 intronic variant exhibited developmental delay and maturity defects.Importantly,we found that TBR1,a gene also implicated in ASD,was significantly increased in neurons carrying the CHD7 intronic variant,suggesting the intrinsic relevance among ASD genes.Furthermore,the morphological defects found in neurons carrying CHD7 intronic mutations were rescued by knocking down TBR1,indicating that TBR1 may be responsible for the defects in CHD7-related disorders.Finally,the CHD7 intronic variant generated three abnormal forms of transcripts through alternative splicing,which all exhibited loss-of-function in functional assays.Our study provides crucial evidence supporting the notion that the intronic variant of CHD7 is potentially an autism susceptibility site,shedding new light on identifying the functions of intronic variants in genetic studies of autism. | Ran Zhang Hui He Bo Yuan Ziyan Wu Xiuzhen Wang Yasong Du Yuejun Chen Zilong Qiu | 2021 | Neuroscience Bulletin2021，37，8： | 1 |
| 8 | What is an effective cardiopulmonary resuscitation training mode?显示文摘To the Editor:Out-of-hospital cardiac arrest(OHCA)is a serious threat to people's lives and safety.The annual incidence of OHCA in North America is approximately 55/100,000,and it is approximately 59/100,000 in Asia.[1,2]How can the success rate of resuscitation be improved in patients with cardiac arrest?The American Heart Association(AHA)and the European Resuscitation Council have put forward their own cardiopulmonary resuscitation(CPR)guidelines,which are constantly updated with the latest research evidence.CPR training is also an important part of the guidelines.[3,4]Chinese colleagues also try to improve the success rate of rescuing patients with cardiac arrest based on the experience of their international counterparts and combine it with Chinese characteristics.CPR training is in full swing,which includes the training of first responders and medical staff. | Xiaojun He Yuefeng Ma Zilong Li Junhui Zhang Jinjun Zhang Jun Liang | 2022 | Chinese Medical Journal2022，，9： | 1 |
| 9 | Selection for Cheaper Amino Acids Drives Nucleotide Usage at the Start of Translation in Eukaryotic Genes显示文摘Coding regions have complex interactions among multiple selective forces,which are manifested as biases in nucleotide composition.Previous studies have revealed a decreasing GC gradient from the 5′-end to 3′-end of coding regions in various organisms.We confirmed that this gradient is universal in eukaryotic genes,but the decrease only starts from the~25th codon.This trend is mostly found in nonsynonymous(ns)sites at which the GC gradient is universal across the eukaryotic genome.Increased GC contents at ns sites result in cheaper amino acids,indicating a universal selection for energy efficiency toward the N-termini of encoded proteins.Within a genome,the decreasing GC gradient is intensified from lowly to highly expressed genes(more and more protein products),further supporting this hypothesis.This reveals a conserved selective constraint for cheaper amino acids at the translation start that drives the increased GC contents at ns sites.Elevated GC contents can facilitate transcription but result in a more stable local secondary structure around the start codon and subsequently impede translation initiation.Conversely,the GC gradients at four-fold and two-fold synonymous sites vary across species.They could decrease or increase,suggesting different constraints acting at the GC contents of different codon sites in different species.This study reveals that the overall GC contents at the translation start are consequences of complex interactions among several major biological processes that shape the nucleotide sequences,especially efficient energy usage. | Na L.Gao Zilong He Qianhui Zhu Puzi Jiang Songnian Hu Wei-Hua Chen | 2021 | Genomics, Proteomics & Bioinformatics2021，19，6： | 0 |
| 10 | Was Wuhan the early epicenter of the COVID-19 pandemic?——a critique显示文摘Recently, Worobey et al.(2022) published a report entitled ‘The Huanan Seafood Wholesale Market in Wuhan was the early epicenter of the COVID-19 pandemic' that succinctly summarizes their study [1]. A pre-print version of this study had earlier elicited a series of highprofile media coverages [2,3]. All these reports deliver a social-political message that the Huanan market is the epicenter of COVID-19. | Yanan Cao Lingling Chen Hua Chen Yupeng Cun Xiaofeng Dai Hongli Du Feng Gao Fengbiao Guo Yalong Guo Pei Hao Shunmin He Shunping He XiongLei He Zheng Hu Boon-Peng Hoh Xin Jin Qian Jiang Qinghua Jiang Asifullah Khan Hong-Zhi Kong Jinchen Li Shuai Cheng Li Ying Li Qiang Lin Jianquan Liu Qi Liu Jian Lu Xuemei Lu Shujin Luo Qinghua Nie Zilong Qiu Tieliu Shi Xiaofeng Song Jianzhong Su Sheng-ce Tao Chaolong Wang Chuan-Chao Wang Guo-Dong Wang Jiguang Wang Qi Wu Shaoyuan Wu Shuhua Xu Yu Xue Wenjun Yang Zhaohui Yang Kai Ye Yuan-Nong Ye Li Yu Fangqing Zhao Yiqiang Zhao Weiwei Zhai Dandan Zhang Liye Zhang Houfeng Zheng Qi Zhou Tianqi Zhu Ya-ping Zhang | 2023 | National Science Review2023，10，4： | 0 |
| 11 | Dynamic simulation of China’s water-grain-meat system and evaluation of its support capability based on water footprint theory显示文摘The supply capacity of water resources,food and meat products is of great importance to the people’s livelihood of a country.In recent years,although China has introduced many policies on water resources and grain production,the current situation of China’s food self-sufficiency and imbalance between supply and demand of water resources has not been fundamentally changed.Food security and water security are facing a serious situation.This paper takes the water footprint as the connection point,and combines the water food meat system with mutual influence,mutual causation and dynamic feedback into a composite system.At the same time,the simulation model of the composite system is established by using system dynamics,and the dynamic simulation of water grain meat in China from 2000 to 2050 is carried out to explore the current situation and future development trend of China’s water,grain and meat supply capacity.It was found that during the simulated period,the agricultural blue-green water footprint on the demand side would continue to dominate,followed by the gray water footprint.The blue water footprint on the supply side remained stable,whereas the green water footprint and the circulating water footprint showed an upward trend.According to the contemporary social and economic development and the model of water resources in China,there will be no meat shortage in the future,but issues have been found in the ability to guarantee water and food supply.The root of China’s food support capability problem is excessive grain consumption due to meat production,whereas the cause of the water support capability problem is the slow development of the water conservation.Food support capability issues can be solved by regulating the meat output of livestock farming and fishery operations,reducing excess production capacity,and stabilizing the meat supply and demand.To solve the water support capability issue,China should focus on accelerating the pace of agricultural water-conservation development,improving the sewage treatment system,building rainwater-collection projects,and promoting the research and development of water recycling technology.This study provides support for optimizing the structures of the meat and grain industries and the policy formulation of the efficient use of water resources in China. | Qiuxiang Jiang Haoyu Liao Xingtao Ouyang Qiang Fu Xiaolong He Yunxing Wu Zilong Wang | 2023 | International Journal of Agricultural and Biological Engineering2023，16，5： | 0 |
| 12 | Computed tomography-based delta-radiomics enabling early prediction ofshort-term responses to concurrent chemoradiotherapy for patients withnon-small cell lung cancer显示文摘Objective:To explore the potential of computed tomography(CT)-based delta-radiomics in predicting early shortterm responses to concurrent chemoradiotherapy for patients with non-small cell lung cancer(NSCLC),in order to determine the optimal time point for the prediction.Methods:A total of 20 patients with pathologically confirmed NSCLC were prospectively enrolled in this study,who did not receive surgical treatment between February 2021 and February 2022.For each case,a total of 1,210 radiomic features(RFs)were extracted from both planning CT(pCT)images along with each of the subsequent three weeks of CT images.EffectiveΔRFs were selected using intra-class correlation coefficient(ICC)analysis,Pearson's correlation,ANOVA test(or Mann-Whitney U-test),and univariate logistic regression.The area under the curve(AUC)of the receiver operating characteristic(ROC)curve was used to evaluate the potential to predict short-term responses of different time points.Results:Among the 1,210ΔRFs for 1-3 weeks,121 common features were retained after processing using ICC analysis and Pearson's correlation.These retained features included 54 and 58 of all time points that differed significantly between the response and non-response groups for the first and third months,respectively(P<0.05).After univariate logistic regression,11 and 44 features remained for the first and third months,respectively.Finally,eightΔRFs(P<0.05,AUC=0.77-0.91)that can discriminate short-term responses in both at 1 and 3 months with statistical accuracy were identified.Conclusion:CT-based delta-radiomics has the potential to provide reasonable biomarkers of short-term responses to concurrent chemoradiotherapy for NSCLC patients,and it can help improve clinical decisions for early treatment adaptation. | Fengqin Zhou Jianping Bi Shen Wu Yi Ding Jun Chen Mengting Yuan Yaoyao He Guang Han Zilong Yuan | 2023 | Radiation Medicine and Protection2023，4，4： | 0 |
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