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| 1 | Caenorhabditis elegans mom-4 is required for the activation of the p38 MAPK signaling pathway in the response to Pseudomonas aeruginosa infection显示文摘The p38 mitogen-activated protein kinase(MAPK)plays an evolutionarily conserved role in the cellular re-sponse to microbial infection and environmental stress.Activation of p38 is mediated through phosphorylation by upstream MAPKK,which in turn is activated by MAPKKK.In the Caenorhabditis elegans,the p38 MAPK(also called PMK-1)signaling pathway has been shown to be required in its resistance to bacterial infection.However,how different upstream MAP2Ks and MAP3Ks specifically contribute to the activation of PMK-1 in response to bacterial infection still is not clearly un-derstood.By using double-stranded RNA-mediated interference(RNAi)and genetic mutants of C.elegans,we demonstrate that C.elegans MOM-4,a mammalian TAK1 homolog,is required for the resistance of C.elegans to a P.aeruginosa infection.We have also found that the MKK-4 of C.elegans is required for P.aeruginosa resistance,but not through the regulation of DLK-1.In summary,our results indicate that different upstream MAPKKKs or MAPKKs regulate the activation of PMK-1 in response to P.aeruginosa. | Ajing Xu Guojun Shi Feng Liu Baoxue Ge | 2013 | Protein & Cell2013，4，1： | 3 |
| 2 | Defective arginine metabolism impairs mitochondrial homeostasis in Caenorhabditis elegans显示文摘Arginine catabolism involves enzyme-dependent reactions in both mitochondria and the cytosol,defects in which may lead to hyperargininemia,a devastating developmental disorder.It is largely unknown if defective arginine catabolism has any effects on mitochondria.Here we report that normal arginine catabolism is essential for mitochondrial homeostasis in Caenorhabditis elegans.Mutations of the arginase gene argn-1 lead to abnormal mitochondrial enlargement and reduced adenosine triphosphate(ATP)production in C elegans hypodermal cells.ARGN-1 localizes to mitochondria and its loss causes arginine accumulation,which disrupts mitochondrial dynamics.Heterologous expression of human ARGl or ARG2 rescued the mitochondrial defects of argn-1 mutants.Importantly,genetic inactivation of the mitochondrial basic amino acid transporter SLC-25A29 or the mitochondrial glutamate transporter SLC-25A18.1 fully suppressed the mitochondrial defects caused by argn-1 mutations.These findings suggest that mitochondrial damage probably contributes to the pathogenesis of hyperargininemia and provide clues for developing therapeutic treatments for hyperargininemia. | Ruofeng Tang Xin Wang Junxiang Zhou Fengxia Zhang Shan Zhao Qiwen Gan Liyuan Zhao Fengyang Wang Qian Zhang Jie Zhang Guodong Wang Chonglin Yang | 2020 | Journal of Genetics and Genomics2020，47，3： | 2 |
| 3 | Suppression of a core metabolic enzyme dihydrolipoamide dehydrogenase (dld ) protects against amyloid beta toxicity in C.elegans model of Alzheimer’s disease显示文摘A decrease in energy metabolism is associated with Alzheimer’s disease(AD),but it is not known whether the observed decrease exacerbates or protects against the disease.The importance of energy metabolism in AD is reinforced by the observation that variants of dihydrolipoamide dehydrogenase(DLD),is genetically linked to late-onset AD.To determine whether DLD is a suitable therapeutic target,we suppressed the dld-1 gene in Caenorhabditis elegans that express human Ab peptide in either muscles or neurons.Suppression of the dld-1 gene resulted in significant restoration of vitality and function that had been degraded by Ab pathology.This included protection of neurons and muscles cells.The observed decrease in proteotoxicity was associated with a decrease in the formation of toxic oligomers rather than a decrease in the abundance of the Ab peptide.The mitochondrial uncoupler,carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone(FCCP),which like dld-1 gene expression inhibits ATP synthesis,had no significant effect on Ab toxicity.Proteomics data analysis revealed that beneficial effects after dld-1 suppression could be due to change in energy metabolism and activation of the pathways associated with proteasomal degradation,improved cell signaling and longevity.Thus,some features unique to dld-1 gene suppression are responsible for the therapeutic benefit.By direct genetic intervention,we have shown that acute inhibition of dld-1 gene function may be therapeutically beneficial.This result supports the hypothesis that lowering energy metabolism protects against Ab pathogenicity and that DLD warrants further investigation as a therapeutic target. | Waqar Ahmad Paul R.Ebert | 2021 | Genes & Diseases2021，8，6： | 2 |
| 4 | Insulin-like signaling pathway functions in integrative response to an olfactory and a gustatory stimuli in Caenorhabditis elegans显示文摘Animals integrate various environmental stimuli within the nervous system to generate proper behavioral responses.However,the underlying neural circuits and molecular mechanisms are largely unknown.The insulinlike signaling pathway is known to regulate dauer formation,fat metabolism,and longevity in Caenorhabditis elegans(C.elegans).Here,we show that this highly conserved signaling pathway also functions in the integrative response to an olfactory diacetyl and a gustatory Cu^(2+) stimuli.Worms of wild-type N2 Bristol displayed a strong avoidance to the Cu^(2+) barrier in the migration pathway to the attractive diacetyl.Mutants of daf-2(insulin receptor),daf-18(PTEN lipid phosphatase),pdk-1(phosphoinositide-dependent kinase),akt-1/-2(Akt/PKB kinase)and sgk-1(serum-and glucocorticoidinducible kinase)show severe defects in the elusion from the Cu^(2+) .Mutations in DAF-16,a forkhead-type transcriptional factor,suppress the integrative defects of daf-2 and akt-1/-2 mutants.We further report that neither cGMP nor TGFβpathways,two other dauer formation regulators,likely plays a role in the integrative learning.These results suggest that the insulin-like signaling pathway constitutes an essential component for sensory integration and decision-making behavior plasticity. | Ya-Ming Jiu Yang Yue Song Yang Lin Liu Jun-Wei Yu Zheng-Xing Wu Tao Xu | 2010 | Protein & Cell2010，1，1： | 1 |
| 5 | C.elegans中dyn-1基因对寿命和发育的影响显示文摘细胞极性对于细胞的多样性起着很重要的作用。发动蛋白是一个大的GTP酶,作用于胞吞作用和肌动蛋白的动力学过程。C.elegans中发动蛋白的同源基因dyn-1起着维持早期细胞极性的功能。我们对C.elegans中dyn-1基因进行了克隆,并构建到表达载体和RNAi载体中。经IPTG诱导表达得到了约90 kDa的DYN-1融合蛋白。同时,利用RNAi方法研究了dyn-1基因沉默后对三种线虫虫株N2、daf-2(e1370)和daf-16(e1038)寿命的影响。C.elegans在喂食dyn-1 RNAi食物后寿命明显缩短,也会导致严重的不育和胚胎致死。 | 曹蕾 杨玉荣 | 2012 | 中国细胞生物学学报2012，34，8： | 1 |
| 6 | 细菌介导的RNA干扰对C.elegans中par-3基因的作用显示文摘设计并构建了针对par-3基因的发夹RNA载体,将构建好的质粒转入大肠杆菌HT115,25℃喂食Caenorhabditiselegans(C.elegans)野生型虫体,24h后观察par-3(RNA干扰)C.elegans的胚胎发育情况。结果显示通过喂食形成发夹结构dsRNA的细菌可以对C.elegans中par-3基因进行RNA干扰,干扰率可以达到60%以上。干扰后的早期胚胎发育丧失第一次卵裂的不对称性,第二次卵裂的纺锤体方向发生改变,与par-3突变体的观察结果一致,为进一步在mex-3转基因虫体中通过RNA干扰研究基因表达打下了基础。 | 杨玉荣 高雅君 | 2006 | 细胞生物学杂志2006，28，4： | 1 |
| 7 | Synthesis,nematicidal evaluation,and SAR study of benzofuran derivatives containing 2-carbonyl thiophene显示文摘Plant-parasitic nematodes are major threat for crop protection.The lack of nematicides with new mode of action and increasing resistance raises the need for novel nematicides.In order to seek new nematicidal lead,originating from the structure of chalcone,a series of fused ring compounds was obtained by ring closure design strategy.These compounds were modified further.The nematicidal activity against M.incognita of synthesized compounds was evaluated.The bioassay showed that compound 3 and some of its derivatives such as compounds 18,19,21,22,23,24 and 26 exhibited excellent nematicidal activity.Among them,compound 23 exhibited significant bioactivity.The LC50/72 hvalue reached 3.20 mg/L in vitro and the inhibition rate was 100.00%at 40 mg/L in the matrix.The structure-activity relationship of synthesized compounds was discussed in details.The influence of compound 23 on egg hatching,motility,and feeding behavior of C.elegans was also evaluated. | Xiaofeng Cao Die Qiu Ruifeng Zhang Zhong Li Xiaoyong Xu | 2023 | Chinese Chemical Letters2023，34，5： | 0 |
| 8 | A genome-wide RNAi screen identifies genes regulating the formation of P bodies in C. elegans and their functions in NMD and RNAi显示文摘Cytoplasmic processing bodies,termed P bodies,are involved in diverse post-transcriptional processes including mRNA decay,nonsense-mediated RNA decay(NMD),RNAi,miRNA-mediated translational repression and storage of translationally silenced mRNAs.Regulation of the formation of P bodies in the context of multicellular organisms is poorly understood.Here we describe a systematic RNAi screen in C.elegans that identified 224 genes with diverse cellular functions whose inactivations result in a dramatic increase in the number of P bodies.83 of these genes form a complex functional interaction network regulating NMD.We demonstrate that NMD interfaces with many cellular processes including translation,ubiquitin-mediated protein degradation,intracellular trafficking and cytoskeleton structure.We also uncover an extensive link between translation and RNAi,with different steps in protein synthesis appearing to have distinct effects on RNAi efficiency.Moreover,the intracellular vesicular trafficking network plays an important role in the regulation of RNAi.A subset of genes enhancing P body formation also regulate the formation of stress granules in C.elegans.Our study offers insights into the cellular mechanisms that regulate the formation of P bodies and also provides a framework for system-level understanding of NMD and RNAi in the context of the development of multicellular organisms. | Yinyan Sun Peiguo Yang Yuxia Zhang Xin Bao Jun Li Wenru Hou Xiangyu Yao Jinghua Han Hong Zhang | 2011 | Protein & Cell2011，2，11： | 0 |
| 9 | A Comprehensive Understanding of Dietary Effects on C.elegans Physiology显示文摘Diet has been shown to play an important role in human physiology.It is a predominant exogenous factor regulating the composition of gut microbiota,and dietary intervention holds promise for treatment of diseases such as obesity,type 2 diabetes,and malnutrition.Furthermore,it was reported that diet has significant effects on physiological processes of C.elegans,including reproduction,fat storage,and aging.To reveal novel signaling pathways responsive to different diets,C.elegans and its bacterial diet were used as an interspecies model system to mimic the interaction between host and gut microbiota.Most signaling pathways identified in C.elegans are highly conserved across different species,including humans.A better understanding of these pathways can,therefore,help to develop interventions for human diseases.In this article,we summarize recent achievements on molecular mechanisms underlying the response of C.elegans to different diets and discuss their relevance to human health. | Jie-jun ZHOU Lei CHUN Jian-feng LIU | 2019 | Current Medical Science2019，39，5： | 0 |
| 10 | High uric acid model in Caenorhabditis elegans显示文摘To establish experimental high uric acid model in C.elegans.Hypoxanthine,adenine,xanthine,and uric acid were used to treat C.elegans and then hyperuricemic C.elegans was evaluated by allopurinol.Hyperuricemic C.elegans were obtained after normal worms were treated by xanthine(0.25 mg/mL,18 h).For hyperuricemic worms,there was a statistically significant increase in the uric acid level(p<0.001)and a lower drug damage(p>0.05).Moreover,the model was proved to keep a high uric acid level for up to 12 h.After given allopurinol(0.25 mg/mL,12 h),the uric acid of hyperuricemic C.elegans had a significant reduction by 15%.Furthermore,xanthine oxidase activity in hyperuricemic C.elegans showed a statistically significant increase(p<0.001),which resulted in a raised uric acid content.A high uric acid model with low drug damage and high efficiency and stability was established in C.elegans after simply xanthine treatment. | Zhenjing Li Yibin Xue Nifei Wang Jingli Cheng Xiaoying Dong Qingbin Guo Changlu Wang | 2019 | Food Science and Human Wellness2019，8，1： | 0 |
| 11 | Cell-based allometry:an approach for evaluation of complexity in morphogenesis显示文摘Background:Morphogenesis is a complex process in a developing animal at the organ,cellular and molecular levels.In this investigation,allometry at the cellular level was evaluated.Methods:Geometric information,including the time-lapse Cartesian coordinates of each cell's center,was used for calculating the allometric coefficients.A zero-centroaxial skew-symmetrical matrix(CSSM),was generated and used for constructing another square matrix(basic square matrix:BSM),then the determinant of BSM was calculated(d).The logarithms of absolute d(Lad)of cell group at different stages of development were plotted for all of the cells in a range of development stages;the slope of the regression line was estimated then used as the allometric coefficient.Moreover,the lineage growth rate(LGR)was also calculated by plotting the Lad against the logarithm of the time.The complexity index at each stage was calculated.The method was tested on a developing Caenorhabditis elegans embryo.Results:We explored two out of the four first generated blastomeres in C.elegans embryo.The ABp and EMS lineages show that the allometric coefficient of ABp was higher than that of EMS,which was consistent with the complexityindexas well asLGR.Conclusion:The conclusion of this study is that the complexity of the differentiating cells in a developing embryo can be evaluated by allometric scaling based on the data derived from the Cartesian coordinates of the cells at different stages of development. | Ali Tarihi Mojtaba Tarihi Taki Tiraihi | 2023 | Quantitative Biology2023，11，2： | 0 |
| 12 | An Online Computer Vision System for Worm's Quantitative Locomotion Analysis显示文摘Background: The small nematode Caenorhabditis elegans (C.elegans) is an excellent model organism for studying the molecular and cellular basis of nervous system function. Since | Hai-ning Zhang1, Zheng-xing Wu1, An-lian Qu1, Tao Xu2 1 Huazhong University of Science and Technology, Wuhan 430074, P.R.China 2 Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, P.R.China | 2009 | 生物物理学报2009，25，S1： | 0 |
| 13 | Probing lysosomal activity in vivo显示文摘Lysosomes are membrane-bound organelles for biomolecule degradation and recycling.They also serve as a nutrient sensing and signaling center to maintain cell and tissue homeostasis.Lysosomal properties alter in response to developmental or environmental cues,but these changes are hard to track in vivo.Employing C.elegans as a model system,we have developed assays to examine and quantify lysosome properties in vivo,including lysosome maturation,acidification and cleavage activity.These assays can be used to reveal alterations of lysosomal activity during C.elegans development and in stress conditions. | Xin Li Yanan Sun Xiaochen Wang | 2021 | Biophysics Reports2021，7，1： | 0 |
| 14 | A pair of transporters controls mitochondrial Zn^(2+) levels to maintain mitochondrial homeostasis显示文摘Zn^(2+)is required for the activity of many mitochondrial proteins,which regulate mitochondrial dynamics,apoptosis and mitophagy.However,it is not understood how the proper mitochondrial Zn^(2+)level is achieved to maintain mitochondrial homeostasis.Using Caenorhabditis elegans,we reveal here that a pair of mitochondrion-localized transporters controls the mitochondrial level of Zn^(2+).We demonstrate that SLC-30A9/ZnT9 is a mitochondrial Zn^(2+)exporter.Loss of SLC-30A9 leads to mitochondrial Zn^(2+)accumulation,which damages mitochondria,impairs animal development and shortens the life span.We further identify SLC-25A25/SCaMC-2 as an important regulator of mitochondrial Zn^(2+)import.Loss of SLC-25A25 suppresses the abnormal mitochondrial Zn^(2+)accumulation and defective mitochondrial structure and functions caused by loss of SLC-30A9.Moreover,we reveal that the endoplasmic reticulum contains the Zn^(2+)pool from which mitochondrial Zn^(2+)is imported.These findings establish the molecular basis for controlling the correct mitochondrial levels for normal mitochondrial structure and functions. | Tengfei Ma Liyuan Zhao Jie Zhang Ruofeng Tang Xin Wang Nan Liu Qian Zhang Fengyang Wang Meijiao Li Qian Shan Yang Yang Qiuyuan Yin Limei Yang Qiwen Gan Chonglin Yang | 2022 | Protein & Cell2022，13，3： | 0 |
| 15 | An investigation of changes in water quality throughout the drinking water production/distribution chain using toxicological and fluorescence analyses显示文摘Changes in water quality from source water to finished water and tap water at two conventional drinking water treatment plants(DWTPs)were monitored.Beside the routine water quality testing,Caenorhabditis elegans-based toxicity assays and the fluorescence excitation–emission matrices technique were also applied.Both DWTPs supplied drinking water that met government standards.Under current test conditions,both the investigated finished water and tap water samples exhibited stronger lethal,genotoxic and reprotoxic potential than the relative source water sample,and the tap water sample was more lethal but tended to be less genotoxic than the corresponding finished water sample.Meanwhile,the nearly complete removal of tryptophan-like substances and newly generated tyrosine-like substances were observed after the treatment of drinking water,and humic-like substances were identified in the tap water.Based on these findings,toxic pollutants,including genotoxic/reproductive toxicants,are produced in the drinking water treatment and/or distribution processes.Moreover,further studies are needed to clarify the potentially important roles of tyrosine-like and humic-like substances in mediating drinking water toxicity and to identify the potential sources of these contaminants.Additionally,tryptophan-like fluorescence may be adopted as a useful parameter to monitor the treatment performance of DWTPs.Our observations provided insights into the importance of utilizing biotoxicity assays and fluorescence spectroscopy as tools to complement the routine evaluation of drinking water. | Xue Han Xin Ji Xuan Ma Jun-Ling Liu Zhen-Yu He Wei Chang Fei Tang Ai-Lin Liu | 2020 | Journal of Environmental Sciences2020，32，1： | 0 |
| 16 | The SNAPc complex mediates starvation-induced trans-splicing in Caenorhabditis elegans显示文摘Dietary restriction usually suppresses biosynthesis but activates catabolic pathways in animals.However,the short-term starvation enhances biosynthetic activities and promotes ribosomal biogenesis in adult Caenorhabditis elegans.The mechanism underlying the processes remains largely unknown.Here,we find that the short-term starvation enhances the SL1 trans-splicing of translation-related genes in adult C.elegans by transcriptome analysis.The small nuclear RNA-activating protein complex(SNAPc)promotes SL RNA production and mediates starvation-induced trans-splicing.TOFU-5,a core factor in the upstream sequence transcription complex(USTC)essential for piRNA production,is also involved in the starvationinduced trans-splicing processes.Knocking down components of the SNAPc complex and tofu-5 extends worm survival under starvation conditions.Taken together,our study highlights the importance of SL transsplicing in the nutrition response and reveals a mechanism of the survival regulation by food deprivation via SNAPc and TOFU-5. | Xinhao Hou Chengming Zhu Mingjing Xu Xiangyang Chen Cheng Sun Björn Nashan Shouhong Guang Xuezhu Feng | 2022 | Journal of Genetics and Genomics2022，49，10： | 0 |
| 17 | Realgar transforming solution is involved of JNK, P38 MAPK pathway in down-regulating Ras/MAPK pathway in Caenorhabditis elegans显示文摘OBJECTIVE To investigate the effect and the mechanisms of realgar transforming solution(RTS)on down-regulating over activated ras.METHODS we used the optimizing technical processes to obtain the RTS,and eval⁃uate the mechanisms of RTS on down-regulating overactivated ras in Caenorhabditis elegans.RESULTS We found that the mRNA level of let60 and lin45 significantly decreased following exposure to RTS,but mRNA levels of mpk1 were not statistically significant in let60/ras(gf)mutant.RTS together with sorafenib(RAF inhibitors)significantly enhanced the activity of RTS on down-regulating overactivated ras more than RTS only,but 50μmol·L^-1 PD98059,a specific ERK inhibitor did not.Lin45 gene RNAi decreased the ability of RTS on down-regulating overactivated ras significantly,but mpk1 gene RNAi did not,indicating that the activity of RTS on down-regulating overactivated ras mainly through targeting to lin45/raf.In addition,RTS significantly increased mRNA level of pmk1/p38 and jnk1/jnk in let-60/ras(gf)mutant,50μmol·L^-1 SB203580(p38 inhibitor)and SP600125(JNK inhibitor)significantly attenuated the effects of RTS on down-regulating overactivated ras in some degree,and pmk1,jnk1 gene RNAi displayed the similar results,suggesting that P38 and JNK/MAPK pathways in some degree were involved in the effects of RTS on down-regulating overactivated ras in C.ele⁃gans.CONCLUSION Realgar transforming solution down-regulate the Ras/MAPK pathway through JNK and P38 MAPK pathways. | LIU Dong-ling ZHI De-juan WAN Feng-qi WANG Meng-qi ZHOU Ting BAI Yan-li LI Hong-yu | 2019 | 中国药理学与毒理学杂志2019，33，9： | 0 |
| 18 | Particle selectivity of filtering by C. elegans显示文摘A nematode Caenorhabditis elegans(C. elegans) is a filter feeder, which draws a suspension of bacteria and separates bacteria from the solvent by using pharyngeal pumping motions and specific mouth parts. This mechanism has not been fully understood. We investigated the mechanism of filtering of bacteria in the pharynx of C. elegans by visualization by fluorescent particles and dyed E. coli. We succeeded in quantifying the selectivity of bacteria-sized particles by C. elegans. The most accumulated particles were those of 0.5 μm in diameter. The quantity of accumulated particles of 0.2 μm or 1.0 μm in diameter was about one third of that of particles of 0.5μm in diameter. The least accumulated particles were those of 0.05 μm in diameter. These results suggest that the pharyngeal structures of C. elegans would be suitable for eating bacteria because the size of bacteria ingested by C. elegans worms is about 0.5 μm in diameter. We also succeeded in visualizing pharyngeal structures and pumping motions and flow in the pharynx. We found that there were phase differences in the motions among procorpus, metacorpus and isthmus. This result suggests filtering would occur at the two tips of procorpus and isthmus by the phase differences. We found that bacteria-sized particles and bacteria were flowed and trapped in the channels, which existed along the central lumen from tip of procorpus to isthmus. From our results, we proposed the novel mechanism of filtering of bacteria through the channels for flowing and trapping. In future, this selective filtering mechanism of C. elegans would be applied to development of microfluidic filtration devices for medical and biological equipment. | Yuki Suzuki Kenji Kikuchi Keiko Tsuruta-Numayama Takuji Ishikawa | 2019 | Theoretical & Applied Mechanics Letters2019，9，2： | 0 |
| 19 | Mutations in β-G spectrin disrupt the DCVs excytosis in C. elegans显示文摘β spectrin is a major component of the membrane skeleton, a structure found at the plasma membrane of most animal cells. In C. elegans, the genome encodes only one | Ya-Ming Jiu, Xian-Guang Lin, Jun-Wei Yu, Zheng-Xing Wu College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, P.R.China | 2009 | 生物物理学报2009，25，S1： | 0 |
| 20 | A novel N^(6)-Deoxyadenine methyltransferase METL-9 modulates C.elegans immunity via dichotomous mechanisms显示文摘N_(6)-Methyldeoxyadenine(6mA)has been rediscovered as a DNA modification with potential biological function in metazoans.However,the physiological function and regulatory mechanisms regarding the establishment,maintenance and removal of 6mA in eukaryotes are still poorly understood.Here we show that genomic 6mA levels change in response to pathogenic infection in Caenorhabditis elegans(C.elegans).We further identify METL-9 as the methyltransferase that catalyzes DNA 6mA modifications upon pathogen infection.Deficiency of METL-9 impairs the induction of innate immune response genes and renders the animals more susceptible to pathogen infection.Interestingly,METL-9 functions through both 6mA-dependent and-independent mechanisms to transcriptionally regulate innate immunity.Our findings reveal that 6mA is a functional DNA modification in immunomodulation in C.elegans. | Chengchuan Ma Tingling Xue Qi Peng Jie Zhang Jialiang Guan Wanqiu Ding Yi Li Peixue Xia Liankui Zhou Tianyu Zhao Sheng Wang Li Quan Chuan-Yun Li Ying Liu | 2023 | Cell Research2023，33，8： | 0 |
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