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| 1 | Nerve growth factor pretreatment against glutamate-induced hippocampal neuronal injury Action mechanism of phosphatase and tensin homologue deleted on chromosome 10显示文摘BACKGROUND: Nerve growth factor (NGF) attenuates glutamate-induced injury to hippocampal neurons, and the human tumor suppressor gene phosphatase and tensin homologue deleted on chromosome 10 (PTEN) promotes neuronal apoptosis. However, effects of PTEN in NGF-mediated neuroprotection against glutamate excitotoxicity remain poorly understood. OBJECTIVE: To investigate the relationship between NGF inhibition of glutamate-induced injury and PTEN. DESIGN, TIME AND SETTING: The randomized, controlled, in vitro study was performed at the Department of Pathophysiology, Medical School of Nantong University, China from October 2007 to March 2008. MATERIALS: Glutamate, NGF, 4, 6-diamidino-2-phenyl-indolediacetate, 3-[4, 5-dimethylthiazol-2-yl]- 2, 5-diphenyl tetrazoliumbromide (MTT), and lactate dehydrogenase kit (Sigma, USA), fluorescence microscope and inverted phase contrast microscope (Olympus, Japan) were used in this study. METHODS: Hippocampal neurons were obtained from newborn (< 24 hours) Sprague Dawley rats and cultured for 7 days. The control group was not treated with any intervention factor, the glutamate group was treated with glutamate (0.2 mmol/L), and NGF groups were treated with NGF (10, 50, 100, and 200 μg/L, respectively) prior to glutamate treatment. MAIN OUTCOME MEASURES: The MTT and lactate dehydrogenase assays were applied to evaluate viability of hippocampal neurons. Morphological changes in hippocampal neurons were observed using an inverted phase-contrast microscope, and neuronal apoptosis was detected by 4, 6-diamidino-2-phenyl-indolediacetate staining. PTEN mRNA and protein expression were measured by reverse transcription-polymerase chain reaction and Western blot analysis, respectively. RESULTS: Glutamate (0.2 mmol/L) induced significantly decreased neuronal viability and greater lactate dehydrogenase efflux compared with the control group (P < 0.01). However, compared with the glutamate group, cell viability significantly increased and lactate dehydrogenase efflux decreased in the NGF group with increasing NGF concentrations (P < 0.05 or P < 0.01). The apoptotic ratio and PTEN mRNA and protein expression decreased in the NGF group compared with the glutamate group (P < 0.01). CONCLUSION: Pretreatment with NGF exerted neuroprotective effects against glutamate-induced injury, partially through inhibition of PTEN expression and neuronal apoptosis. | Yae Hu Jiahui Mao Yan Zhu Ailing Zhou | 2010 | Neural Regeneration Research2010，5，1： | 12 |
| 2 | Biofilm from a clinical strain of Cryptococcus neoformans activates the NLRP3 inflammasome显示文摘 | Guowei Lei Mingkuan Chen Hua Li Jun-Ling Niu Shuxian Wu Liming Mao Ailing Lu Hongbin Wang Wei Chen Bin Xu Qibin-Leng Cunshuan Xu Guiwen Yang Liguo An Li-Ping Zhu Guangxun Meng | 2013 | Cell Research2013，23，7： | 5 |
| 3 | Introduction of a multicenter online database for non-metastatic breast cancer in China显示文摘Dear Editor,Breast cancer is now the most frequently diagnosed cancer and is the fifth leading cause of cancer-related death in Chinese women. Therefore, the burden of breast cancer in China is gradually increasing. According to figures released by the Chinese Cancer Center in 2018, the number of newly diagnostic breast cancer is about 278,900 cases, accounting for 16.51%of all women who were diagnosed with the first primary malignant tumors;and 66,000 cases of breast cancer died in 2014 (Chen et al., 2016). | Yaping Yang Jieqiong Liu Min Peng Fengxi Su Xiaoming Xie Zhenzhen Liu Jundong Wu Wei Wei Dongxian Zhou Weiwen Li Ailing Zhang Guosen Su Weixiong Yang Jishang Chen Dekui Ma Yongguang Cai Kai Chen Liling Zhu Qiang Liu Erwei Song | 2020 | Science China(Life Sciences)2020，63，9： | 4 |
| 4 | Hydroxyl radical formation upon dark oxidation of reduced iron minerals: Effects of iron species and environmental factors显示文摘Dark formation of hydroxyl radical upon oxidation of reduced iron minerals plays an important role in the degradation and transformation of organic and inorganic pollutants.Herein,we compared the hydroxyl radical formation from various reduced iron minerals at different redox conditions.·OH production was generally observed from the oxidation of reduced iron minerals,following the order:mackinawite(FeS)>reduced nontronite(iron-bearing smectite clay)> pyrite(FeS2)> side rite(FeCO3).Structural Fe^2+ and dissolved O2 play critical roles in ·OH production from reduced iron minerals.·OH production increases with decreasing pH,and Cl^-has little effect on this process.More importantly,dissolved organic matter significantly enhances ·OH production,especially under O2 purging,highlighting the importance of this process in ambient environments.This sunlight-independent pathway in which ’OH forms during oxidation of reduced iron minerals is helpful for understanding the degradation and transformation of various inorganic and organic pollutants in the redox-fluctuation environments. | Ailing Zhu Yingying Guo Guangliang Liu Maoyong Song Yong Liang Yong Cai Yongguang Yin | 2019 | Chinese Chemical Letters2019，30，12： | 2 |
| 5 | Comparative Study of Ship-in-a-Bottle and Anchored Heterogenized Mn Complexes显示文摘 | Yang Guoyu Sun Ailing Zhou Wenfeng Zhu Hailin Jiang Denggao | 2007 | Catalysis Letters (-)2007，，3： | 1 |
| 6 | A non-ACE2 competing human single-domain antibody confers broad neutralization against SARS-CoV-2 and circulating variants显示文摘The current COVID-19 pandemic has heavily burdened the global public health system and may keep simmering for years.The frequent emergence of immune escape variants have spurred the search for prophylactic vaccines and therapeutic antibodies that confer broad protection against SARS-CoV-2 variants.Here we show that the bivalency of an affinity maturated fully human singledomain antibody(n3113.1-Fc)exhibits exquisite neutralizing potency against SARS-CoV-2 pseudovirus,and confers effective prophylactic and therapeutic protection against authentic SARS-CoV-2 in the host cell receptor angiotensin-converting enzyme 2(ACE2)humanized mice.The crystal structure of n3113 in complex with the receptor-binding domain(RBD)of SARS-CoV-2,combined with the cryo-EM structures of n3113 and spike ecto-domain,reveals that n3113 binds to the side surface of up-state RBD with no competition with ACE2.The binding of n3113 to this novel epitope stabilizes spike in up-state conformations but inhibits SARS-CoV-2 S mediated membrane fusion,expanding our recognition of neutralization by antibodies against SARS-CoV-2.Binding assay and pseudovirus neutralization assay show no evasion of recently prevalent SARS-CoV-2 lineages,including Alpha(B.1.1.7),Beta(B.1.351),Gamma(P.1),and Delta(B.1.617.2)for n3113.1-Fc with Y58L mutation,demonstrating the potential of n3113.1-Fc(Y58L)as a promising candidate for clinical development to treat COVID-19. | Zhenlin Yang Yulu Wang Yujia Jin Yuanfei Zhu Yanling Wu Cheng Li Yu Kong Wenping Song Xiaolong Tian Wuqiang Zhan Ailing Huang Shanshan Zhou Shuai Xia Xiaoxu Tian Chao Peng Cuicui Chen Yibing Shi Gaowei Hu Shujuan Du Yuyan Wang Youhua Xie Shibo Jiang Lu Lu Lei Sun Yuanlin Song Tianlei Ying | 2021 | Signal Transduction and Targeted Therapy2021，6，12： | 1 |
| 7 | A 'turn-on' fluorescent chemosensor for aluminum ion and cell imaging application显示文摘 | Ailing Guo Ruitao Zhu Yuehong Ren etal | 2016 | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy2016，153，： | 1 |
| 8 | Native de- fects and Pr impurities in orthorhombic CaTiO3 by first-princi- ples calculations显示文摘 | Zhu Ailing Wang Jianchuan Zhao Dongdong | 2011 | Physica B: Condensed Matter2011，406，13： | 1 |
| 9 | A general and facile calcination method to synthesize single-site catalysts for highly efficient electrochemical CO_(2) reduction显示文摘The electrochemical CO_(2) reduction reaction(CO_(2)RR)has received widespread attention as a promising method for producing sustainable chemicals and mitigating the global warming.Here,we demonstrate a general and facile synthetic route for the metal-nitrogen-carbon(M-N-C)type catalyst by simply calcinating metal acetate and urea with commercial carbon black,which have potential application in CO_(2)RR.The synthesized Ni-NC-600 catalyst has the structure of single Ni atom coordinated with one N atom and three C atoms(Ni-N_(1)C_(3)),which is suggested by X-ray absorption spectroscopy.The Ni-NC-600 catalyst exhibits high CO_(2)RR catalytic performance and a high CO Faraday efficiency above 98%in a wide potential range from-0.7 to-1.3 V(vs.reversible hydrogen electrode(RHE)),superior to most of the reported Ni-N-C catalysts.This work has developed a facile strategy to synthesize high performance CO_(2)RR catalyst. | Rui Sui Bingyan Wang Yongsheng Wang Jiajing Pei Wei Zhu Wenxing Chen Chunhui Li Ailing Sun Zhongbin Zhuang | 2024 | Nano Research2024，17，5： | 0 |
| 10 | Dithizone-functionalized C18 online solid-phase extraction-HPLC-ICP-MS for speciation of ultra-trace organic and inorganic mercury in cereals and environmental samples显示文摘A simple and efficient dithizone-functionalized solid-phase extraction(SPE)procedure,online coupled with high-performance liquid chromatography(HPLC)-inductively coupled plasma mass spectrometry,was developed for the first time for enrichment and determination of ultra-trace mercury(Hg)species(inorganic divalent Hg(Hg(Ⅱ)),methylmercury(CH_(3)Hg(Ⅱ))and ethylmercury(C_(2)H_(5)Hg(Ⅱ))in cereals and environmental samples.In the proposed method,functionalization of the commercial C_(18) column with dithizone,enrichment,and elution of the above Hg species can be completed online with the developed SPE device.A simple solution of 2-mercaptoethanol(1%(V/V))could be used as an eluent for both the SPE and HPLC separation of Hg species,significantly simplifying the method and instrumen-tation.The online SPE method was optimized by varying dithizone dose,2-mercaptoethanol concentration,and sample volume.In addition,the effect of pH,coexisting interfering ions,and salt effect on the enrichment was also discussed.Under the optimized conditions,the detection limits of Hg species for 5 mL water sample were 0.15 ng/L for Hg(Ⅱ),0.07 ng/L for CH_(3)Hg(Ⅱ),and 0.04 ng/L for C_(2)H_(5)Hg(Ⅱ)with recoveries in the range of 85%-100%.The developed dithizone-functionalized C_(18) SPE column can be reused after a single function-alization,which significantly simplifies the enrichment step.Moreover,the stability of Hg species enriched on the SPE column demonstrated its suitability for field sampling of Hg species for later laboratory analysis.This environment-friendly method offers a robust tool to detect ultra-trace Hg species in cereals and environmental samples. | Ying Wang Ailing Zhu Yingying Fang Changjun Fan Yanli Guo Zhiqiang Tan Yongguang Yin Yong Cai Guibin Jiang | 2022 | Journal of Environmental Sciences2022，34，5： | 0 |
| 11 | Progress of three-dimensional macroporous bioactive glass for bone regeneration显示文摘Bioactive 眼镜(BG ) 由于他们的优秀 osteoconductive, osteoinductive, biocompatible 和可被细菌破坏的性质是为 macroporous 脚手架的理想的材料,并且他们结合的高骨头评价。因为他们能刺激 vascularized 骨头向内长,他们提高在脚手架之间结合并且包围纸巾,用 BG 做的 Macroporous 脚手架在对骨头新生的高需求。工程 BG/biopolymers (BP ) composites 或混血儿可以是与优秀性质准备 macroporous 脚手架的一个好方法。这份报纸在为骨头新生准备三维的 macroporous BG 和 BG/BP 脚手架在过去几年里总结进步。因为 BG 的易碎物是,在开发 macroporous 脚手架和这的一个主要问题在忍受应用程序的负担限制他们的使用, macroporous 脚手架的机械性质特别地在这评论被强调。 | Lijun JI Yunfeng SI Ailing LI Wenjun WANG Dong QIU Aiping ZHU | 2012 | Frontiers of Chemical Science and Engineering2012，6，4： | 0 |
| 12 | Corrigendum to“Dithizone-functionalized C18 online solid-phase extraction-HPLC-ICP-MS for speciation of ultra-trace organic and inorganic mercury in cereals and environmental samples”Journal of Environmental Science 115(2022)403-410显示文摘The authors regretThe authors would like to apologize for any inconvenience caused. | Ying Wang Ailing Zhu Yingying Fang Changjun Fan Yanli Guo Zhiqiang Tan Yongguang Yin Yong Cai Guibin Jiang | 2023 | Journal of Environmental Sciences2023，，2： | 0 |
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