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| 1 | A complete sequence and comparative analysis of a SARS-associated virus(Isolate BJ01)显示文摘The genome sequence of the Severe Acute Respiratory Syndrome (SARS)-associated virus provides essential information for the identification of pathogen(s), exploration of etiology and evolution, interpretation of transmission and pathogenesis, development of diagnostics, prevention by future vaccination, and treatment by developing new drugs. We report the complete genome sequence and comparative analysis of an isolate (BJ01) of the coronavirus that has been recognized as a pathogen for SARS. The genome is 29725 nt in size and has 11 ORFs (Open Reading Frames). It is composed of a stable region encoding an RNA-dependent RNA polymerase (composed of 2 ORFs) and a variable region representing 4 CDSs (coding sequences) for viral structural genes (the S, E, M, N proteins) and 5 PUPs (putative uncharacterized proteins). Its gene order is identical to that of other known coronaviruses. The sequence alignment with all known RNA viruses places this virus as a member in the family of Coronaviridae. Thirty putative substitutions have been identified by comparative analysis of the 5 SARS- associated virus genome sequences in GenBank. Fifteen of them lead to possible amino acid changes (non-synonymous mutations) in the proteins. Three amino acid changes, with predicted alteration of physical and chemical features, have been detected in the S protein that is postulated to beinvolved in the immunoreactions between the virus and its host. Two amino acid changes have been detected in the Mprotein, which could be related to viral envelope formation. Phylogenetic analysis suggests the possibility of non-human origin of the SARS-associated viruses but provides noevidence that they are man-made. Further efforts should focus on identifying the etiology of the SARS-associated virus and ruling out conclusively the existence of otherpossible SARS-related pathogen(s). | QIN E'de ZHU Qingyu YU Man FAN Baochang CHANG Guohui SI Bingyin YANG Bao PENG Wenming JIANG Tao LIU Bohua DENG Yongqiang LIU Hong ZHANG Yu WANG Cui LI Yuquan GAN Yonghua LI Xiaoyu L Fushuang TAN Gang CAO Wuchun, YANG Ruifu Institute of Microbiology and Epidemiology, Chinese Academy of Military Medical Sciences, Beijing 100071, China WANG Jian, LI Wei, XU Zuyuan, LI Yan, WU Qingfa, LIN Wei, CHEN Weijun, TANG Lin, DENG Yajun, HAN Yujun, LI Changfeng, LEI Meng, LI Guoqing, LI Wenjie, L Hong, SHI Jianping, TONG Zongzhong, ZHANG Feng, LI Songgang, LIU Bin, LIU Siqi, DONG Wei, WANG Jun, Gane K-S Wong, YU Jun & YANG Huanming* Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300 National Center for Genome Information, Beijing 101300, China | 2003 | Chinese Science Bulletin2003，48，10： | 121 |
| 2 | Boron neutron capture therapy for malignant melanoma: first clinical case report in China显示文摘A phase Ⅰ/Ⅱ clinical trial for treating malignant melanoma by boron neutron capture therapy(BNCT) was designed to evaluate whether the world's first in-hospital neutron irradiator(IHNI) was qualified for BNCT. In this clinical trial planning to enroll 30 patients, the first case was treated on August 19, 2014. We present the protocol of this clinical trial, the treating procedure, and the clinical outcome of this first case. Only grade 2 acute radiation injury was observed during the first four weeks after BNCT and the injury healed after treatment. No late radiation injury was found during the 24-month follow-up. Based on positron emission tomography-computed tomography(PET/CT) scan, pathological analysis and gross examination, the patient showed a complete response to BNCT,indicating that BNCT is a potent therapy against malignant melanoma and IHNI has the potential to enable the delivery of BNCT in hospitals. | Zhong Yong Zewen Song Yongmao Zhou Tong Liu Zizhu Zhang Yanzhong Zhao Yang Chen Congjun Jin Xiang Chen Jianyun Lu Rui Han Pengzhou Li Xulong Sun Guohui Wang Guangqing Shi Shaihong Zhu | 2016 | Chinese Journal of Cancer Research2016，28，6： | 13 |
| 3 | Decrement of Sugar Consumption in Rice Young Panicle Under High Temperature Aggravates Spikelet Number Reduction显示文摘Two rice genotypes Huanghuazhan(HHZ, heat-resistant) and IR36(heat-susceptible) were subjected to high-temperature(HT, 40℃) and normal-temperature(NT, 32℃) treatments at the spikelet differentiation stage. HT treatment inhibited spikelet differentiation, aggravated spikelet degeneration, reduced spikelet size, and disordered carbohydrate allocation. Meanwhile, HT treatment increased nonstructural carbohydrate content in leaves, but decreased that in stems and young panicles, and the same tendencies of sucrose and starch contents were observed in leaves and stem. However, HT treatment significantly increased the sucrose content and sharply decreased the glucose and fructose contents in young panicles. Lower activity levels of soluble acid invertase(EC3.2.1.26) and sucrose synthase(EC2.4.1.13) were observed under HT treatment. Moreover, HT treatment reduced the activities of key enzymes associated with glycolysis and the tricarboxylic acid cycle, which indicated sucrose consumption was inhibited in young panicles under HT treatment. Exogenous glucose and fructose applied under HT treatment increased the spikelet number more than exogenous sucrose. In conclusion, the results demonstrated that the reduction of spikelet number under high temperature was more affected by the decrease in sugar consumption than the blocking of sucrose transport. The impairment of sucrose hydrolysis was the main reason for the inhibition of sugar utilization. | WANG Yaliang ZHANG Yikai SHI Qinghua CHEN Huizhe XIANG Jing HU Guohui CHEN Yanhua WANG Xiaodan WANG Junke YI Zihao ZHU Defeng ZHANG Yuping | 2020 | Rice science2020，27，1： | 6 |
| 4 | Improved esophageal squamous cell carcinoma screening effectiveness by risk-stratified endoscopic screening:evidence from high-risk areas in China显示文摘Background:Risk-stratified endoscopic screening(RSES),which offers endoscopy to those with a high risk of esophageal cancer,has the potential to increase effectiveness and reduce endoscopic demands compared with the universal screening strategy(i.e.,endoscopic screening for all targets without risk prediction).Evidence of RSES in high-risk areas of China is limited.This study aimed to estimate whether RSES based on a 22-score esophageal squamous cell carcinoma(ESCC)risk prediction model could optimize the universal endoscopic screening strategy for ESCC screening in high-risk areas of China.Methods:Eight epidemiological variables in the ESCC risk prediction model were collected retrospectively from 26,618 individuals aged 40-69 from three high-risk areas of China who underwent endoscopic screening betweenMay 2015 and July 2017.The model’s performance was estimated using the area under the curve(AUC).Participants were categorized into a high-risk group and a low-risk group with a cutoff score having sensitivities of both ESCC and severe dysplasia and above(SDA)at more than 90.0%.Results:The ESCC risk prediction model had an AUC of 0.80(95% confidence interval:0.75-0.84)in this external population.We found that a score of 8(ranging from 0 to 22)had a sensitivity of 94.2% for ESCC and 92.5% for SDA.The RSES strategy using this threshold score would allow 50.6% of endoscopies to be avoided and save approximately US$0.59 million compared to universal endoscopic screening among 26,618 participants.In addition,a higher prevalence of SDA(1.7%vs.0.9%),a lower number need to screen(60 vs.111),and a lower average cost per detected SDA(US$3.22 thousand vs.US$5.45 thousand)could have been obtained by the RSES strategy.Conclusions:The RSES strategy based on individual risk has the potential to optimize the universal endoscopic screening strategy in ESCC high-risk areas of China. | He Li Chao Ding Hongmei Zeng Rongshou Zheng Maomao Cao Jiansong Ren Jufang Shi Dianqin Sun Siyi He Zhixun Yang Yiwen Yu Zhe Zhang Xibin Sun Guizhou Guo Guohui Song Wenqiang Wei Wanqing Chen Jie He | 2021 | Cancer Communications2021，41，8： | 3 |
| 5 | Measurement of Neutron Capture Cross Section for ^(180)Hf | Chen Jinxiang Shi Zhaomin Tang Guoyou Zhang Guohui Institute of Heavy Ion Physics,Peking University,Beijing 100871Lu Hanlin Han Xiaogang Huang Xiaolong China Institute of Atomic Energy,P.O.Box 275(3),Beijing 102413 | 1997 | Chinese journal of nuclear physics1997，，2： | 3 |
| 6 | Comparative analysis of whole-genome sequences of Streptococcus suis显示文摘The outbreak of Streptococcus suis re-cently in some districts of Sichuan Province in China has caused over 30 deaths and over 200 infections in human beings. In order to study the pathogenicity mechanism and to prevent the bacteria from spreading and infecting human beings and swine, we have annotated and analyzed the genomes of two strains, Streptococcus suis P1/7 and 89-1591 re-spective1y. The whole length of P1/7 is 2.007 Mb, and has 1969 ORFs. In contrast, the partial genome sequence of 89-1591 is 1.98 Mb in length and exists in 177 contigs with 1918 ORFs. Analysis shows that the average lengths of CDSs in two genomes are very close, and the numbers of the homolog ORFs are 1306 between those two strains. Most of the tox-icity factors of the two strains are homologeous, but there are still some significant differences between those two strains. For example, among the 11 genes (cps2A―cps2K) encoding for the capsules in P1/7, 4 (cps2A, 2B, 2I, 2J) are not detected in strain 89-1591. At the same time, the genes encoding EF and Haemolysin in P1/7 are also not found in strain 89-1591. Besides, the genes related to DNA replica-tion, repair and recombination differ from each other significantly and there also exist certain differences among the surface proteins. Those characteristics indicate that those two strains have evolved their ownspecific functions to adapt to the different environ-ments and that the pathogenesis of the two strains is different. We have accumulated comprehensive ge-nomics information for future systematic studies of S. sui. Our results are helpful for disease prevention, vaccine development, as well as drug design for S. suis. | WEI Wu DING Guohui WANG Xiaojing SUN dingchun TU Kang HAO Pei WANG Chuan CAO Zhiwei SHI Tieliu LI Yixue | 2006 | Chinese Science Bulletin2006，51，10： | 2 |
| 7 | One-pot controlled synthesis of sea-urchin shaped Bi2S3/CdS hierarchical heterostructures with excellent visible light photocatalytic activity显示文摘 | Shi Yunhan Chen Yajie Tian Guohui | 2014 | Dalton Transactions2014，43，12： | 1 |
| 8 | Cellular particle swarm optimization显示文摘 | Yang Shi Hongcheng Liu Liang Gao Guohui Zhang | 2010 | Information Sciences2010，，20： | 1 |
| 9 | PtSerpin from the swimming crab Portunus trituberculatus , a putative regulator of prophenoloxidase activation with antibacterial activity显示文摘 | Yuan Liu Guohui Shi Zhaoxia Cui Danli Luo Chengwen Song Xihong Li Min Hui Yingdong Li | 2014 | Fish and Shellfish Immunology2014，，： | 1 |
| 10 | A new machine vi-sion system with visual attention function显示文摘 | Shi Jinfang Su Zhenwei Li Guohui | 2010 | Journal of In-formation and Computational Science2010，7，14： | 1 |
| 11 | A new ma- chine vision system with visual attention function 显示文摘 | Shi Jinfang Su Zhenwei Li Guohui | 2010 | Journal of Information and Computational Science2010，7，14： | 1 |
| 12 | Correlates of Sexual Lifestyles Among Older Adults Living With HIV in China: Findings From the Sexual Well-being (SWELL) Study显示文摘Sexual lifestyles are closely related to overall human health and well-being.Few studies have focused on sexual lifestyles among older adults living with HIV(OALHIV),especially in low-and middle-income countries.This study is a part of the sexual well-being among older adults in China(SWELL)study,which is a multicenter cross-sectional study focused on sexual health among older adults aged 50 years and older.Participants were 680 OALHIV(≥50 years old)from the SWELL study conducted from June 2020 to December 2022.Data were collected through one-on-one interviews.We used logistic regression to assess the correlates of sexual activity and sexual satis-faction.Among all participants,37.1%were sexually active.Being older,male,in a steady relationship and employed were associated with being sexually active.The prevalence of sexual satisfaction was 69.8%among sexually active OALHIV.Being homosexual and reporting a better general health status were associated with sexual satisfaction.The OALHIV who have depressive symptoms were less likely to report sexual satisfaction.To support holistic healthy aging among OALHIV,it is important for healthcare providers to be educated about the importance of enquiring about sexual activity,satisfaction and problems and addressing concerns while conveying sex-positive attitudes during clinical reviews,as these are still critical aspects of their health and well-being. | Bingyi Wang Xin Peng Leiwen Fu Xinyi Li Tongxin Shi Bowen Liang Tian Tian Thomas Fitzpatrick Zhen Lu Weijie Zhang JunyeBian Xin Xiao YingWang Yunlong Ao Lin Ouyang YongCai Maohe Yu Guohui Wu Linghua Li Xiaojun Meng Huachun Zou | 2023 | Infectious Microbes & Diseases2023，5，3： | 1 |
| 13 | A Novel Var- iable Neighborhood Genetic Algorithm for Multi- objective Flexible Job-shop Scheduling Problems 显示文摘 | Zhang Guohui Gao Liang Shi Yang | 2010 | Materials and Product Technologies: Ad- vanced Materials Research2010，，： | 1 |
| 14 | An effective genetic algorithm for the flexible job-shop scheduling problem显示文摘 | Guohui Zhang Liang Gao Yang Shi | 2011 | Expert Systems with Applicatio ns2011，4，38： | 1 |
| 15 | A genetic algorithm and tabu search for solving flexible job shop schedules 显示文摘 | Guohui Zhang Yang Shi Liang Gao | 2008 | International Symposium on Computational Intelligence and Design2008，，： | 1 |
| 16 | An effective ge- netic algorithm for the flexible job-shop scheduling problem 显示文摘 | ZHANG Guohui GAO Liang YANG Shi | 2011 | Expert Systems with Applications2011，38，4： | 1 |
| 17 | Long-range gene regulation network of the MGMT enhancer modulates glioma cell sensitivity to temozolomide显示文摘Approximately 80%of primary tumors in the central nervous system are malignant glioma,and>50%of those are diagnosed as glioblastoma(GBM),the most aggressive glioma.Despite treatment advances,GBM prognosis is unsatisfying,and the median survival time of patients is only 14-17 months. | Bohan Chen Anshun He Jinfang Bi Shupeng Sun Yiping Ma Wenbin Wang Dianhao Guo Jun Chen Yuyang Qian Tengfei Shi Guohui Nie Zhongfang Zhao Jiandang Shi Hongzhen Yang Lei Zhang Wange Lu | 2021 | Journal of Genetics and Genomics2021，48，10： | 1 |
| 18 | Determination of trace glucose and forecast of human diseases by affinity adsorption solid substrate room temperature phosphorimetry based on Triticum valgaris lectin labeled with 4.0-generation dendrimers显示文摘 | Zhiming Li Guohui Zhu Jiaming Liu Qiaomei Lu Minlan Yang Hong Wu Xiumei Shi Xinhua Chen | 2007 | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy2007，，5： | 1 |
| 19 | dentification of mutations in patients with acquired pure red cell aplasia显示文摘 | Xinchao Zhang Yi Shi Lingjun Song Chang Shen Qi Cai Zhou Zhang Jun Wu Guohui FU Weiwei Shen | 2018 | Acta Biochimica et Biophysica Sinica2018，50，7： | 1 |
| 20 | Hyperandrogenism in POMCa-deficient zebrafish enhances somatic growth without increasing adiposity显示文摘The endocrine regulatory roles of the hypothalamic-pituitary-adrenocortical axis on anxiety-like behavior and metabolic status have been found throughout animal taxa.However,the precise effects of the balancing adrenal corticosteroid biosynthesis under the influence of adrenocorticotrophic hormone(ACTH),a pro-opiomelanocortin(POMC)-derived peptide,on animal energy expenditure and somatic growth remain unknown.POMC has also been identified as one of the candidate loci for polycystic ovary syndrome,which features hyperandrogenism and some prevalence of obesity in patients.Here we show that zebrafish lacking functional POMCa exhibit similar phenotypes of stress response and body weight gain but not obesity as observed in mammalian models.In contrast with the impaired anorexigenic signaling cascade of melanocyte-stimulating hormones and leptin,which are responsible for their obesity-prone weight gain observed in various pome mutant mammals,analyses with our pomca mutant series indicate that ACTH is the key regulator for the phenotype with enhanced somatic growth without obesity in pomca-deficient zebrafish.Hypocortisolism associated with hyperandrogenism has been observed in the pomca-deficient zebrafish,with enhanced activation of mammalian target of rapamycin complex 1;reutilization of amino acids and fatty acid^-oxidation are observed in the muscle tissue of the pomca-deficient fish.After reducing hyperandrogenism by crossing our pomca mutant fish with a cy p l 7a 1-deficient background,the phenotype of enhanced somatic growth in pomca-deficient fish was no longer observed.Thus,our work also demonstrated that the role of POMCa in stress response seems to be conserved in vertebrates,whereas its effect on adipostasis is unique to teleosts. | Chuang Shi Yao Lu Gang Zhai Jianfei Huang Guohui Shang Qiyong Lou Dongliang Li Xia Jin Jiangyan He Zhenyu Du Jianfang Gui Zhan Yin | 2020 | Journal of Molecular Cell Biology2020，12，4： | 1 |
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