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| 1 | CKIP-1 regulates macrophage proliferation by inhibiting TRAF6-mediated Akt activation显示文摘巨噬细胞在开发,动态平衡,织物修理和免疫起枢轴的作用。巨噬细胞增长被刺激殖民地的因素(M-CSF ) 导致了 Akt 发信号的巨噬细胞支持;然而,这个过程怎么被终止,仍然保持不清楚。这里,我们作为巨噬细胞增长的一个新奇禁止者识别酷蛋白 kinase 2-interacting protein-1 (CKIP-1 ) 。在放松巨噬细胞, CKIP-1 是在由组成地活跃的 GSK3β 的丝氨酸 342 点的 phosphorylated;, Akt 的下游的目标。这 phosphorylation 触发 CKIP-1 的 polyubiquitination 和 proteasomal 降级。在 M-CSF 刺激之上, Akt 被 CSF-1R-PI3K 激活然后使 GSK3β 失去活性;,导致 CKIP-1 和 β 的稳定; -catenin 蛋白质。β -catenin 包括 cyclin D 和 c-Myc 支持增长基因的表示。CKIP-1 与 TRAF6,为连接 K63 的 ubiquitination 要求的 ubiquitin ligase 和 Akt 的血浆膜招募交往,并且终止调停 TRAF6 的 Akt 激活。由这个工具, CKIP-1 在 M-CSF 刺激以后在迟了的阶段明确地禁止巨噬细胞增长。而且, CKIP-1 缺乏在老鼠自发地开发的 vitro 和 CKIP-1 −/− 导致增加的增长和巨噬细胞的减少的 apoptosis 巨噬细胞主导的脾大和 myeloproliferation。一起,这些数据证明 CKIP-1 由禁止调停 TRAF6 的 Akt 激活在巨噬细胞动态平衡的规定起一个关键作用。 | Luo Zhang Yiwu Wang Fengjun Xiao Shaoxia Wang Guichun Xing Yang Li Xiushan Yin Kefeng Lu Rongfei Wei Jiao Fan Yuhan Chen Tao Li Ping Xie Lin Yuan Lei Song Lanzhi Ma Lujing Ding Fuchu He Lingqiang Zhang | 2014 | Cell Research2014，24，6： | 7 |
| 2 | Spatio-temporal variability of phytoplankton assemblages and its controlling factors in spring and summer in the Subei Shoal of Yellow Sea, China显示文摘The Subei Shoal is a special coastal area with complex physical oceanographic properties in the Yellow Sea.In the present study,the distribution of phytoplankton and its correlation with environmental factors were studied during spring and summer of 2012 in the Subei Shoal of the Yellow Sea.Phytoplankton species composition and abundance data were accomplished by Utermohl method.Diatoms represented the greatest cellular abundance during the study period.In spring,the phytoplankton cell abundance ranged from 1.59×10^3 to 269.78×10^3 cell/L with an average of 41.80×10^3 cell/L,and Skeletonema sp.and Paralia sulcata was the most dominant species.In summer,the average phytoplankton cell abundance was 72.59×10^3 cell/L with the range of 1.78×10^3 to 574.96×10^3 cell/L,and the main dominant species was Pseudo-nitzschia pungens,Skeletonema sp.,Dactyliosolen fragilissima and Chaetoceros curvisetus.The results of a redundancy analysis(RDA)showed that turbidity,temperature,salinity,pH,dissolved oxygen(DO),the ratio of dissolved inorganic nitrogen to silicate and SiO4-Si(DIN/SiO4-Si)were the most important environmental factors controlling phytoplankton assemblages in spring or summer in the Subei Shoal of the Yellow Sea. | Yuanzi Huo Honghua Shi Jianheng Zhang Qiao Liu Yuanliang Duan Qing He Kefeng Yu Hongsheng Bi Chunlei Fan Peimin He | 2019 | Acta Oceanologica Sinica2019，38，10： | 4 |
| 3 | A Multifunctional Anti-Proton Electrolyte for High-Rate and Super-Stable Aqueous Zn-Vanadium Oxide Battery显示文摘Large volumetric expansion of cathode hosts and sluggish transport kinetics in the cathode–electrolyte interface,as well as dendrite growth and hydrogen evolution at Zn anode side are considered as the system problems that cause the electrochemical failure of aqueous Zn-vanadium oxide battery.In this work,a multifunctional anti-proton electrolyte was proposed to synchronously solve all those issues.Theoretical and experimental studies confirm that PEG 400 additive can regulate the Zn^(2+) solvation structure and inhibit the ionization of free water molecules of the electrolyte.Then,smaller lattice expansion of vanadium oxide hosts and less associated by-product formation can be realized by using such electrolyte.Besides,such electrolyte is also beneficial to guide the uniform Zn deposition and suppress the side reaction of hydrogen evolution.Owing to the integrated synergetic modifica-tion,a high-rate and ultrastable aqueous Zn-V_(2)O_(3)/C battery can be constructed,which can remain a specific capacity of 222.8 m Ah g^(-1)after 6000 cycles at 5 A g^(-1),and 121.8 m Ah g^(-1) even after 18,000 cycles at 20 A g^(-1),respectively.Such“all-in-one”solution based on the electrolyte design provides a new strategy for developing high-performance aqueous Zn-ion battery. | Yangwu Chen Dingtao Ma Kefeng Ouyang Ming Yang Sicheng Shen Yanyi Wang Hongwei Mi Lingna Sun Chuanxin He Peixin Zhang | 2022 | Nano-Micro Letters2022，14，9： | 3 |
| 4 | Efficacy of rigosertib, a small molecular RAS signaling disrupter for the treatment of KRAS-mutant colorectal cancer显示文摘Objective:Mutant KRAS,the principal isoform of RAS,plays a pivotal role in the oncogenesis of colorectal cancer by constitutively activating the RAF/MEK/ERK and PI3K/AKT pathways.Effective targeted therapies are urgently needed.We investigated whether rigosertib,a benzyl styryl sulfone RAS signaling disruptor,could selectively kill KRAS-mutant colorectal cancer cells.Methods:CCK-8 was used to determine the cell viability.Patient-derived tumor and cancer cell xenograft models were used to detect the inhibitory efficacy of rigosertib.Flow cytometry was used to evaluate the apoptosis and cell cycle progression.Apoptosis and cell cycle arrest markers were detected by Western blot.DCFH-DA was used to determine the reactive oxygen species.Immunohistochemistry staining and Western blot were performed to characterize RAS signaling markers in colorectal cancer tissues and cells.Results:Rigosertib(RGS)exhibited a cytotoxic effect against colorectal cancer cells,which was greater in KRAS-mutant cells.Furthermore,RGS induced mitotic arrest and oxidative stress-dependent apoptosis in KRAS-mutant DLD1 and HCT116 cells.Besides,RGS disrupted RAS signaling,and the inhibition of RAS/MEK/ERK was independent of cellular oxidative stress.Using patient-derived xenograft models,the response and tumor inhibition of RGS were significantly higher in the KRAS-mutant subgroup,while p-MEK,p-ERK,and p-AKT levels of RGS-treated tumors were significantly decreased.Finally,in a KRAS-mutant,chemotherapy-resistant patient-derived xenograft model,RGS showed a stronger therapeutic effect than the combination standard therapy involving fluoropyrimidine+oxaliplatin/irinotecan+bevacizumab.Conclusions:These data showed that targeting RAS signaling using RGS could be a therapeutic treatment for KRAS-mutant colorectal cancer patients. | Xinyi Zhou Qian Xiao Dongliang Fu Haochen Zhang Yang Tang Jinjie He Yeting Hu Xiangxing Kong Fei Teng Xiangrui Liu Ying Yuan Kefeng Ding | 2022 | Cancer Biology & Medicine2022，19，2： | 2 |
| 5 | A two-dimensional spectrum for general bistatic SAR processing显示文摘 | YANG Kefeng HE Feng LIANG Diannong | 2010 | IEEE Geosci Remote Sens Lett2010，7，10： | 1 |
| 6 | A two-dimensional spectrum for general bistatic SAR processing 显示文摘 | YANG Kefeng HE Feng LIANG Diannong | 2010 | IEEE Geosci Remote Sens Lett2010，7，10： | 1 |
| 7 | Objective evaluation method for image fusion based on image quality index显示文摘 | Hu Liangmei Gao Juan He Kefeng | 2004 | Acta Electronica Sinica2004，32，12： | 1 |
| 8 | Dynamic Contrast-Enhanced CT Characterization of Xp11.2 Translocation/TFE3 Gene Fusions versus Papillary Renal Cell Carcinomas显示文摘 | Jian He Kefeng Zhou Bin Zhu Gutian Zhang Xiaogong Li Hongqian Guo Weidong Gan Zhengyang Zhou Tian Liu Yi-Xiang Wang | 2015 | BioMed Research International2015，，： | 1 |
| 9 | Paclitaxel-loaded nanoparticles decorated with anti-CD133 antibody: a targeted therapy for liver cancer stem cells显示文摘 | Cheng Jin Zhaoxu Yang Jingyue Yang Haimin Li Yong He Jiaze An Ling Bai Kefeng Dou | 2014 | Journal of Nanoparticle Research2014，，1： | 1 |
| 10 | Hierarchical Capillary Coating to Biofunctionlize Drug-Eluting Stent for Improving Endothelium Regeneration显示文摘The drug-eluting stent(DES)has become one of the most successful and important medical devices for coronary heart disease,but yet suffers from insufficient endothelial cell(EC)growth and intima repair,eventually leading to treatment failure.Although biomacromolecules such as vascular endothelial growth factor(VEGF)would be promising to promote the intima regeneration,combining hydrophilic and vulnerable biomacromolecules with hydrophobic drugs as well as preserving the bioactivity after harsh treatments pose a huge challenge.Here,we report on a design of hierarchical capillary coating,which composes a base solid region and a top microporous region for incorporating rapamycin and VEGF,respectively.The top spongy region can guarantee the efficient,safe,and controllable loading of VEGF up to 1μg/cm^(2) in 1 minute,providing a distinctive real-time loading capacity for saving the bioactivity.Based on this,we demonstrate that our rapamycin-VEGF hierarchical coating impressively promoted the competitive growth of endothelial cells over smooth muscle cells(ratio of EC/SMC~25)while relieving the adverse impact of rapamycin to ECs.We further conducted the real-time loading of VEGF on stents and demonstrate that the hierarchical combination of rapamycin and VEGF showed remarkable endothelium regeneration while maintaining a very low level of in-stent restenosis.This work paves an avenue for the combination of both hydrophobic and hydrophilic functional molecules,which should benefit the next generation of DES and may extend applications to diversified combination medical devices. | Jing Wang Yunfan Xue Jun Liu Mi Hu He Zhang Kefeng Ren Yunbing Wang Jian Ji | 2020 | Research2020，，1： | 1 |
| 11 | Hyperbolic mean curvature flow显示文摘 | He Chunlei Kong Dexing Liu Kefeng | 2009 | J Differential Equations2009，246，： | 1 |
| 12 | Puerarin, isolated from Kudzu root (Willd.), attenuates hepatocellular cytotoxicity and regulates the GSK-3β/NF-κB pathway for exerting the hepatoprotection against chronic alcohol-induced liver injury in rats显示文摘 | Rong Li Tao Liang Qiaoling He Chao Guo Lingyuan Xu Kefeng Zhang Xiaoqun Duan | 2013 | International Immunopharmacology2013，，： | 1 |
| 13 | Ubiquitin ligase Smurf1 targets TRAF family proteins for ubiquitination and degradation显示文摘 | Shan Li Kefeng Lu Jian Wang Liguo An Guiwen Yang Hui Chen Yu Cui Xiushan Yin Ping Xie Guichun Xing Fuchu He Lingqiang Zhang | 2010 | Molecular and Cellular Biochemistry (-)2010，，1： | 1 |
| 14 | Screening and engineering of high-activity promoter elements through transcriptomics and red fluorescent protein visualization in Rhodobacter sphaeroides显示文摘The versatile photosyntheticα-proteobacterium Rhodobacter sphaeroides,has recently been extensively engineered as a novel microbial cell factory(MCF)to produce pharmaceuticals,nutraceuticals,commodity chemicals and even hydrogen.However,there are no well-characterized high-activity promoters to modulate gene transcription during the engineering of R.sphaeroides.In this study,several native promoters from R.sphaeroides JDW-710(JDW-710),an industrial strain producing high levels of co-enzyme Q10(Q10)were selected on the basis of transcriptomic analysis.These candidate promoters were then characterized by using gusA as a reporter gene.Two native promoters,Prsp_7571 and Prsp_6124,showed 620%and 800%higher activity,respectively,than the tac promoter,which has previously been used for gene overexpression in R.sphaeroides.In addition,a Prsp_7571-derived synthetic promoter library with strengths ranging from 54%to 3200%of that of the tac promoter,was created on the basis of visualization of red fluorescent protein(RFP)expression in R.sphaeroides.Finally,as a demonstration,the synthetic pathway of Q10 was modulated by the selected promoter T334*in JDW-710;the Q10 yield in shake-flasks increased 28%and the production reached 226 mg/L.These well-characterized promoters should be highly useful in current synthetic biology platforms for refactoring the biosynthetic pathway in R.sphaeroides-derived MCFs. | Tong Shi Lu Zhang Mindong Liang Weishan Wang Kefeng Wang Yue Jiang Jing Liu Xinwei He Zhiheng Yang Haihong Chen Chuan Li Dongyuan Lv Liming Zhou Biqin Chen Dan Li Li-Xin Zhang Gao-Yi Tan | 2021 | Synthetic and Systems Biotechnology2021，6，4： | 0 |
| 15 | Tolerance and acceptance of hepatic venous pressure gradient measurement in cirrhosis(CHESS1904):An international multicenter study显示文摘Aim:To determine the tolerance and acceptance of hepatic venous pressure gradient(HVPG)measurements in patients with liver cirrhosis.Methods:This prospective international multicenter study included 271 patients with cirrhosis who were scheduled to undergo HVPG measurement between October 2019 and June 2020.Data related to the tolerance and acceptance of HVPG measurements were collected using descriptive questionnaires.Results:HVPG measurements were technically successful in all 271 patients,with 141(52.0%)undergoing HVPG measurement alone.The complication rate was 0.4%.Postoperative pain was significantly lower than preoperative expected pain(p<0.001)and intraoperative pain(p<0.001),and intraoperative pain was also significantly lower than preoperative expected pain(p=0.036).No,mild,moderate,severe,and intolerable discomfort scores were reported by 36.9%,44.6%,11.1%,6.3%,and 0.4%of these patients,respectively,during HVPG measurement and by 54.6%32.5%,11.4%,1.5%,and 0%,respectively,after HVPG measurement.Of these patients,39.5%had little understanding and 10%had no understanding of the value of HVPG measurement,with 35.1%and 4.1%regarding HVPG measurements as being of little or no help,respectively.Most patients reported that they would definitely(15.5%),probably(46.9%),or possibly(29.9%)choose to undergo additional HVPG measurements again,and 62.7%regarded the cost of the procedure as acceptable.Conclusion:HVPG measurement was safe and well‐tolerated in patients with cirrhosis,but patient education and communication are warranted to improve the acceptance of this procedure. | Jun‐Hui Sun He Zhao Haijun Zhang Lei Li NecatiÖrmeci Zi‐Niu Yu Xun Li Shuangxi Li Xujun Yang Huaping Wei Xiaoliang Zhu Zhengcong Zhang Yajin Wang Zhongwei Zhao Jianting Mao Qiaohong Wu Xiaole Sun Huiling Xiang Kefeng Jia Chao Yang Wei Wu Xiuqing Lin Haixin Yao Changzeng Zuo Jitao Wang Bo Zhang Chunqing Zhang Xiaoling Wu Guangchuan Wang Shengjuan Yao Ruihang Wang Li Zhou Hui Huan Qingli Tu Xue Pu Feng Zhang Qin Yin Linpeng Zhang Ying Guo Jian Wang Kohei Kotani Sawako Uchida‐Kobayashi Norifumi Kawada He Zhu Li Li Wei Wang Guo Zhang Lei Yu Xudong Cui Qingliang Zhu Hailong Zhang Xiaoli Hu Rafael OXimenes Adriano Gonçalves de Araújo Giulliano Gardenghi Yubao Zheng Zebin Wu Mingsheng Huang Xiaoyong Chen Jun Wu Feng Xie Yang Bo Shengjuan Hu Linke Ma Xiao Li Xiaolong Qi | 2022 | Portal Hypertension & Cirrhosis2022，1，1： | 0 |
| 16 | A rapid method for the detection of humic acid based on the poly(thymine)-templated copper nanoparticles显示文摘This work described a new method for the detection of humic acid(HA) based on the poly(thymine)(polyT)-templated copper nanoparticles(CuNPs). Without the presence of HA, the formation of poly Ttemplated CuNPs could take place, resulting in strong fluorescence emission peaks at 610 nm(upon excitation at 340 nm). On the other hand, when HA was present, strong interaction between HA and Cu^(2+)took place, which then hampered the effective formation of fluorescent CuNPs, leading to the decrease in fluorescence intensity. Furthermore, under the optimal experimental conditions, the method exhibited a high specificity to HA with a detection limit of 0.4 mg/L. This work has demonstrated a low-cost and convenient method that could be accomplished within 10 min. The method could provide a simple, rapid,and sensitive fluorescent platform for the detection of HA. | Changbei Ma Mingjian Chen Haisheng Liu Kefeng Wu Hailun He Kemin Wang | 2018 | Chinese Chemical Letters2018，29，1： | 0 |
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