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| 1 | Honeysuckle-encoded atypical microRNA2911 directly Largets influenza A viruses显示文摘 | Zhen Zhou Xihan Li Jinxiong Liu Lei Dong Qun Chen Jialing Liu Huihui Kongt Qianyi Zhang Xian Qi Dongxia Hou Lin Zhang Guoquan Zhang Yuchen Liu Yujing Zhang Jing Li Jin Wang Xi Chen Hua Wang Junfeng Zhang Hualan Chen Ke Zen Chen-Yu Zhang | 2015 | Cell Research2015，25，1： | 104 |
| 2 | Prevalence of Thyroid Nodules and Its Relationship with Iodine Status in Shanghai:a Population-based Study显示文摘Objective This study was designed to evaluate the prevalence of thyroid nodules(TNs) and its relationship with urine iodine concentrations(UICs) after the regional rapid economic growth and lifestyle changes. Methods A cross-sectional survey was conducted in the general population aged 15-69 years. A questionnaire regarding general and personal characteristics and relevant information was administered. Ultrasonography of the thyroid was performed, and serum triiodothyronine(T3), tetraiodothyronine(T4), serum thyroid stimulating hormone(TSH), free triiodothyronine(FT3), free tetraiodothyronine(FT4), thyroglobulin antibody(Tg Ab), thyroid peroxidase antibody(TPOAb), and TSH receptor antibody(TRAb) levels were measured for each individual subject. Results The prevalence rates of TNs in the whole population, females and males were 27.76%, 34.04%, and 21.60%, respectively. The prevalence of multiple nodules increased with age, whereas the prevalence peaks differed between males and females. The median UICs in the whole population and females with non-TNs were higher than those of subjects with TNs(P=0.0035, P=0.0068). The median UICs in subjects with a single TN were higher than those in subjects with multiple TNs(P=0.0164, P=0.0127). The result showed a U-shaped curve relationship between UIC and prevalence of TNs. The prevalence of TNs was the lowest when the UIC was 140-400 μg/L. Conclusion The prevalence of TNs was nearly 30% and increased with age. The relationship between UIC and prevalence of TNs is U-shaped, with an increase in risk when the UIC was <140 μg/L and >400 μg/L. Very low or high UIC levels need attention and correction. | SONG Jun ZOU Shu Rong GUO Chang Yi ZANG Jia Jie ZHU Zhen Ni MI Ming HUANG Cui Hua YU Hui Ting LU Xi RUAN Ye WU Fan | 2016 | Biomedical and Environmental Sciences2016，29，6： | 25 |
| 3 | Single-cell RNA-seq uncovers dynamic processes and critical regulators in mouse spermatogenesis显示文摘 | Yao Chen Yuxuan Zheng Yun Gao Zhen Lin Suming Yang Tongtong Wang Qiu Wang Nannan Xie Rong Hua Mingxi Liu Jiahao Sha Michael D. Griswold Jinsong Li Fuchou Tang Ming-Han Tong | 2018 | Cell Research2018，28，9： | 26 |
| 4 | Comparison of Undernutrition Prevalence of Children under 5 Years in China between 2002 and 2013显示文摘Objective To describe the undernutrition status of children under 5-year in China, and study the trend between 2002 and 2013. Methods The study was based on two national surveys. Undernutrition was determined against WHO's 2006 growth standards. The prevalence in 2013 and 2002 was weighted by China sixth National Population Census(2010). The relationship between undernutrition and gender/age groups/different areas use weighted logistic regression. Results The results indicated the overall prevalence of stunting, underweight, and wasting of Chinese children under 5-year was 8.1%, 2.4%, and 1.9% in 2013, respectively. The prevalence of stunting was higher for children aged 12-47 month, while underweight was higher for children aged 48-59 month. The prevalence of undernutrition was higher in rural areas than in urban areas, especially in poor rural areas. There was a decline of stunting, underweight, and wasting between 2002 and 2013 among the children, with greater reduction in rural areas than in urban areas. Conclusion The prevalence of undernutrition of children under 5-year remains high in rural areas especially in poor rural areas in China. It is urgent to take action to control undernutrition in the vulnerable areas and subgroups. | YU Dong Mei ZHAO Li Yun YANG Zhen Yu CHANG Su Ying YU Wen Tao FANG Hong Yun WANG Xun YU Dan GUO Qi Ya XU Xiao Li FANG Yue Hui ZHAO Wen Hua YANG Xiao Guang DING Gang Qiang LIANG Xiao Feng | 2016 | Biomedical and Environmental Sciences2016，29，3： | 18 |
| 5 | Impact of Heat Wave in 2005 on Mortality in Guangzhou, China显示文摘Objective To assess the impact of the heat wave in 2005 on mortality among the residents in Guangzhou and to identify susceptible subpopulations in Guangzhou, China. Methods The data of daily number of deaths and meteorological measures from 2003 to 2006 in Guangzhou were used in this study. Heat wave was defined as ≥7 consecutive days with daily maximum temperature above 35.0 ℃ and daily mean temperature above the 97th percentile during the study period. The excess deaths and rate ratio (RR) of mortality in the case period compared with the reference period in the same summer were calculated. Results During the study period, only one heat wave in 2005 was identified and the total number of excess deaths was 145 with an average of 12 deaths per day. The effect of the heat wave on non-accidental mortality (RR=1.23, 95% CI: 1.11-1.37) was found with statistically significant difference. Also, greater effects were observed for cardiovascular mortality (RR=1.34, 95% CI: 1.13-1.59) and respiratory mortality (RR=1.31, 95% CI: 1.02-1.69). Females, the elderly and people with lower socioeconomic status were at significantly higher risk of heat wave-associatedmortality. Conclusion The 2005 heat wave had a substantial impact on mortality among the residents in Guangzhou, particularly among some susceptible subpopulations. The findings from the present study may provide scientific evidences to develop relevant public health policies and prevention measures aimed at reduction of preventable mortality from heat waves. | YANG Jun LIU Hua Zhang OU Chun Quan LIN Guo Zhen DING Yan ZHOU Qin SHEN Ji Chuan CHEN Ping Yan | 2013 | Biomedical and Environmental Sciences2013，26，8： | 16 |
| 6 | Effects of calcined aluminum salts on the advanced dewatering and solidification/stabilization of sewage sludge显示文摘The high moisture content (80%) in the sewage dewatered sludge is the main obstacle to disposal and recycling. A chemical dewatering and stabilization/solidification (S/S) alternative for the sludge was developed, using calcined aluminum salts (AS) as solidifier, and CaCl 2 , Na 2 SO 4 and CaSO 4 as accelerators, to enhance the mechanical compressibility making the landfill operation possible. The properties of the resultant matrixes were determined in terms of moisture contents, unconfined compressive strength, products of hydration, and toxicity characteristics. The results showed that AS exhibited a moderate pozzolanic activity, and the mortar AS 0 obtained with 5% AS and 10% CaSO 4 of AS by weight presented a moisture contents below 50%–60% and a compressive strength of (51.32 ± 2.9) kPa after 5–7 days of curing time, meeting the minimum requirement for sanitary landfill. The use of CaSO 4 obviously improved the S/S performance, causing higher strength level. X-ray diffraction, scanning electron microscopy and thermogravimetry- differential scanning calorimetry investigations revealed that a large amount of hydrates (viz., gismondine and CaCO 3 ) were present in solidified sludge, leading to the depletion of evaporable water and the enhancement of the strength. In addition, the toxicity characteristic leaching procedure (TCLP) and horizontal vibration (HJ 557-2009) leaching test were conducted to evaluate their environmental compatibility. It was found that the solidified products conformed to the toxicity characteristic criteria in China and could be safely disposed of in a sanitary landfill. | Guangyin Zhen Xiaofei Yan Haiyan Zhou Hua Chen Tiantao Zhao Youcai Zhao | 2011 | Journal of Environmental Sciences2011，23，7： | 15 |
| 7 | Liver Enzymes Concentrations Are Closely Related to Pre-diabetes:Findings of the Shanghai Diabetes Study Ⅱ (SHDS Ⅱ)显示文摘Objective To investigate the relationship of liver enzymes with hyperglycemia in a large population in Shanghai and identify the association between liver enzymes and insulin resistance.Methods A total of 3 756 participants were enrolled.Each participant underwent an oral glucose tolerance test and completed a questionnaire.Anthropometric indices were recorded and serum samples were collected for measurement.Results Liver enzymes concentrations were independently associated with i-IGT,IFG+IGT,and diabetes.With the increase of ALT and GGT concentrations,ORs for i-IGT,IFG+IGT,and diabetes increased gradually.By comparing patients in the highest quartile of GGT concentrations or ALT concentrations with those in the lowest quartile (Q1),ORs for i-IGT,IFG+IGT,or diabetes was significant after adjustment.Both ALT and GGT concentrations were linearly correlated with HOMA-IR and independently associated with HOMA-IR [ALT OR (95% CI):2.56 (1.51-4.34) P=0.00;GGT OR (95% CI):2.66 (1.53-4.65) P=0.00].Conclusion Serum ALT and GGT concentrations were closely related to pre-diabetes and diabetes in the Shanghai population and positively associated with insulin resistance. | GAO Fei PAN Jie Min HOU Xu Hong FANG Qi Chen LU Hui Juan TANG Jun Ling GU Hui Lin PAN Zhi Jian YAO You Hua SHEN Wei Zhen JIA Wei Ping | 2012 | Biomedical and Environmental Sciences2012，25，1： | 15 |
| 8 | The Predictive Value of Baseline HBs Ag Level and Early Response for HBs Ag Loss in Patients with HBe Ag-positive Chronic Hepatitis B during Pegylated Interferon Alpha-2a Treatment显示文摘Objective To explore the predictive value of baseline HBsAg level and early response for HBsAg loss in patients with HBeAg-positive chronic hepatitis B during pegylated interferon alpha-2a treatment. Methods A total of 121 patients with HBeAg-positive chronic hepatitis B who achieved HBsAg loss were enrolled; all patients were treated with PEG-IFNα-2a 180 μg/week. Serum HBV DNA and serological indicators(HBsAg, anti-HBs, HBeAg, and anti-HBe) were determined before and every 3 months during treatment. Results The median treatment time for HBsAg loss was 84 weeks(7-273 weeks), and 74.38%(90 cases) of the patients needed extended treatment(> 48 weeks). The correlation between baseline HBsAg levels and the treatment time of HBsAg loss was significant(B = 14.465, t = 2.342, P = 0.021). Baseline HBsAg levels together with the decline range of HBsAg at 24 weeks significantly correlated with the treatment time of HBsAg loss(B = 29.862, t = 4.890, P = 0.000 and B = 27.993, t = 27.993, P = 0.005). Conclusion Baseline HBsAg levels and extended therapy are critical steps toward HBsAg loss. Baseline HBsAg levels together with early response determined the treatment time of HBsAg loss in patients with HBeAg-positive chronic hepatitis B during pegylated interferon alpha-2a treatment. | LI Ming Hui ZHANG Lu QU Xiao Jing LU Yao SHEN Ge LI Zhen Zhen WU Shu Ling LIU Ru Yu CHANG Min HU Lei Ping HUA Wen Hao SONG Shu Jing WAN Gang XIE Yao | 2017 | Biomedical and Environmental Sciences2017，30，3： | 14 |
| 9 | Nogo-A expression dynamically varies after spinal cord injury显示文摘The mechanism involved in neural regeneration after spinal cord injury is unclear. The myelin-derived protein Nogo-A, which is specific to the central nervous system, has been identified to negatively affect the cytoskeleton and growth program of axotomized neurons. Studies have shown that Nogo-A exerts immediate and chronic inhibitory effects on neurite outgrowth. In vivo, inhibitors of Nogo-A have been shown to lead to a marked enhancement of regenerative axon extension. We established a spinal cord injury model in rats using a free-falling weight drop device to subsequently investigate Nogo-A expression. Nogo-A mR NA and protein expression and immunoreactivity were detected in spinal cord tissue using real-time quantitative PCR, immunohistochemistry and western blot analysis. At 24 hours after spinal cord injury, Nogo-A protein and mR NA expression was low in the injured group compared with control and sham-operated groups. The levels then continued to drop further and were at their lowest at 3 days, rapidly rose to a peak after 7 days, and then gradually declined again after 14 days. These changes were observed at both the mR NA and protein level. The transient decrease observed early after injury followed by high levels for a few days indicates Nogo-A expression is time dependent. This may contribute to the lack of regeneration in the central nervous system after spinal cord injury. The dynamic variation of Nogo-A should be taken into account in the treatment of spinal cord injury. | Jian-wei Wang Jun-feng Yang Yong Ma Zhen Hua Yang Guo Xiao-lin Gu Ya-feng Zhang | 2015 | Neural Regeneration Research2015，10，2： | 13 |
| 10 | Seroepidemiological Investigation of Lyme Disease and Human Granulocytic Anaplasmosis among People Living in Forest Areas of Eight Provinces in China显示文摘Objective Lyme disease and Human granulocytic anaplasmosis are tick‐borne diseases caused by Borrelia burgdorferi and Anaplasma phagocytophilum respectively. We have investigated infection and co-infection of the two diseases in the population of forest areas of eight provinces in China by measuring seroprevalence of antibodies against B. burgdorferi and A. phagocytophilum. Methods Forest areas in 8 provinces were chosen for investigation using whole sampling and questionnaire survey methods. 3 669 serum samples from people in the forest areas were tested for the presence of antibodies by indirect immunofluorescent assay (IFA). Results Seroprevalence against B. burgdorferi was 3% to 15% and against A. phagocytophilum was 2% to 18% in the study sites in the 8 provinces in China. We also found co-infection of B. burgdorferi and A. phagocytophilum in 7 of the 8 provinces (the exception being the Miyun area in Beijing). The seroprevalence for both B. burgdorferi and A. phagocytophilum was significantly higher among people exposed to ticks than among people who were not exposed to ticks. Conclusion We conclude that both pathogens are endemic in the forest areas in the eight provinces, but the prevalence of B. burgdorferi and A. phagocytophilum differs between the provinces. | HAO Qin GENG Zhen HOU Xue Xia TIAN Zhen YANG Xiu Jun JIANG Wei Jia SHI Yan ZHAN Zhi Fei LI Guo Hua YU De Shan WANG Hua Yong XU Jian Guo WAN Kang Lin | 2013 | Biomedical and Environmental Sciences2013，26，3： | 13 |
| 11 | Dapper1 promotes autophagy by enhancing the Beclinl -Vps34-Atg14L complex formation显示文摘 | Benvu Ma Weipeng Cao Wenxia Li Chan Gao Zhen Qi Yan Zhao Jun Du Hua Xue Junya Peng Jun Wen Hua Chen Yuanheng Ning Lei Huang Hong Zhang Xiang Gao Li Yu Ye-Guang Chen | 2014 | Cell Research2014，24，8： | 12 |
| 12 | OsBRXL4 Regulates Shoot Gravitropism and Rice Tiller Angle through Affecting LAZY1 Nuclear Localization显示文摘Rice tiller angle is a key agronomic trait that contributes to ideal plant architecture and grain production.LAZY1 (LA1) was previously shown to control tiller angle via affecting shoot gravitropism,but the underlying molecular mechanism remains largely unknown.In this study,we identified an LA1-interacting protein named Brevis Radix Like 4 (OsBRXL4).We showed that the interaction between OsBRXL4 and LA1 occurs at the plasma membrane and that their interaction determines nuclear localization of LA1.We found that nuclear localization of LA1 is essential for its function,which is different from AtLA1,its Arabidopsis ortho.log.Overexpression of OsBRXL4 leads to a prostrate growth phenotype,whereas OsBRXLs RNAi plants,in which the expression levels of OsBRXLI,OsBRXL4,and OsBRXL5 were decreased,display a compact phenotype.Further genetic analysis also supported that OsBRXL4 controls rice tiller angle by affecting nuclear localization of LA1.Consistently,we demonstrated that OsBRXL4 regulates the shoot gravitropism through affecting polar auxin transport as did LA1.Taken together,our study not only identifies OsBRXL4 as a regulatory component of rice tiller angle but also provides new insights into genetic regulation of rice plant architecture. | Zhen Li Yan Liang Yundong Yuan Lei Wang Xiangbing Meng Guosheng Xiong Jie Zhou Yueyue Cai Ningpei Han Lekai Hua Guifu Liu Jiayang Li Yonghong Wang | 2019 | Molecular Plant2019，12，8： | 12 |
| 13 | Comparative Transcriptional Profiling and Preliminary Study on Heterosis Mechanism of Super-Hybrid Rice显示文摘杂种优势比也是从二父母表演的后代改善了的生物现象和优异性能生来的父母线。混合米饭是在利用杂种优势的庄稼的最成功的 apotheoses 之一。由基因表示(半自动地面防空系统)的连续分析 F1 超级混血儿的米饭 Liangyou-2186 和它的父母介绍的 Transcriptional 表明 1183 差别表示了基因( DG ),在哪个之中 DG 被发现显著地在象光合作用和碳固定那样的小径充实,并且大多数涉及碳固定小径的关键基因在 F1 混合米饭展出了起来调整的表示。而且,相应的酶的增加的分解代谢的活动和光合的效率也被检测,它联合了显示那碳固定可能在 F1 混血儿,和力量被提高在超级混血儿的米饭与收益活力和杂种优势被联系。由有收益相关的量的特点 loci (QTL ) 的相关 DG,在微分基因表示和 phenotypic 变化之间的一种潜在的关系也被发现。另外,当以前在 Arabidopsis 报导了,包含生理节奏节奏、轻的发信号的小径的一个规章的网络也被发现,它建议如此的一个网络可能也与在混合米饭的杂种优势是相关的。总的来说,现在的学习为理解在米饭位于杂种优势下面的分子的机制提供另一个看法。 | Gui-Sheng Song Hong-Li Zhai Yong-Gang Peng Lei Zhang Gang Wei Xiao- Ying Chen Yu-Guo Xiao Lili Wang Yue-Jun Chen Bin Wu Bin Chen Yu Zhang Hua Chen Xiu-Jing Feng Wan-Kui Gong Yao Liu Zhi-Jie Yin Feng Wang Guo-Zhen Liu Hong-Lin Xu Xiao-Li Wei Xiao-Ling Zhao Pieter B.F. Ouwerkerk Thomas Hankemeier Theo Reijmers Rob van der Heijden Cong-Ming Lu Mei Wang Jan van der Greef Zhen Zhu | 2010 | Molecular Plant2010，3，6： | 12 |
| 14 | H5N1 influenza virus-specific miRNA-like small RNA increases cytokine production and mouse mortality via targeting poly(rC)-binding protein 2显示文摘H5N1 流行性感冒病毒的感染在所有流行性感冒病毒之中引起最高的死亡。位于如此的高病毒的致病力下面的机制不完全地被理解。这里,我们报导 H5N1 流行性感冒病毒编码象 microRNA 一样小 RNA, miR-HA-3p,它从茎被处理由 Argonaute 2 的包含环的病毒的 RNA 先锋,和戏在在 H5N1 感染期间提高 cytokine 生产的一个角色。miR-HA-3p poly 指向的机械学的学习表演(rC ) 有约束力的蛋白质 2 (PCBP2 ) 并且压制它的表达式。与 PCBP2 being 一致 RIG-I/MAVS-mediated 抗病毒的天生的免疫的一个重要否定管理者,由 miR-HA-3p 的 PCBP2 表示的抑制在感染 H5N1 的人的巨噬细胞和老鼠支持 cytokine 生产病毒。我们断定 miR-HA-3p 是首先识别的流行性感冒编码病毒的象 microRNA 一样功能的 RNA 碎片和贡献导致 H5N1 的 cytokine 暴风雨和死亡的一个新奇毒力因素。 | Xihan Li Zheng Fu Hongwei Liang Yanbo Wang Xian Qi Meng Ding Xinlei Sun Zhen Zhou Ying Huang Hongwei Gu Limin Li Xi Chen Donghai Li Quan Zhao Fenyong Li Hua Wang Jin Wang Ke Zen Chen-Yu Zhang | 2018 | Cell Research2018，28，2： | 12 |
| 15 | Antibacterial Mechanisms of Berberine and Reasons for Little Resistance of Bacteria显示文摘Objective To study the antibacterial mechanisms of berberine and try to understand the reasons why bacteria cells difficultly resisted to it.Methods Detecting the minimal inhibitory concentration(MIC) of bacterial cultures incubated under sub-MIC concentration of berberine,Huanglian,and Neomycin for more than 200 generations,in order to analyze the bacteria resistance.Detecting the binding kinetics of berberine to DNA,RNA,and proteins.Observing the changes in bacterial cell surface structure with scanning electron microscopy.Detecting the Ca2 +and K +released from berberine-treated bacterial cells with atomic absorption spectrum.Detection the absorption of methyl-3H-thymine (3 H-dT),3 H-uridine(3 H-U),and 3 H-tyrosine(3 H-Tyr)into berberine-treated bacterial cells.Results MICs of bacterial cultures,growing more than 200 generations in MH medium with 1/2 MIC of berberine(BA200) or Huanglian(HA200),did not increase compared to the control,while remarkably increased in MH medium with 1/2 MIC of Neomycin (NA200).In addition,from the culture NA200 it was easy to isolate resistant mutant strains which could grow in MH medium with more than four times MIC Neomycin,but from the culture BA200 and HA200 it was difficult to isolate berberine or Huanglian mutant strains could grow in MH medium with more than four times MIC berberine or Huanglian.The binding kinetics of berberine to DNA,RNA,and proteins illustrated that berberine could easily and tightly bind to DNA and RNA,and hardly dis-bind from DNA-and RNA-berberine complexes.Berberine could easily bind to protein too,but also easily dis-bind from berberine-protein complex.The bacterial cells treated with berberine sharply decreased the absorption of 3 H-dT,3 H-U,and 3 H-Tyr,as the radioactive precursors of DNA,RNA,and protein biosynthesis.Berberine could damage bacterial cell surface structure,especially for Gram-negative bacteria.Ca2 +and K + released from berberine-treated cells increased significantly compared to the control.Conclusion All of above results indicate that bacterial cells could not easily become resistant mutants to berberine.The mechanisms for the bactericidal effect of berberine include:inhibiting DNA duplication,RNA transcription,and protein biosynthesis;influencing or inhibiting enzyme activities;destructing the bacterial cell surface structure and resulting in Ca2 +and K +released from cells.All of the berberine bactericidal mechanisms are the most essential physiological functions for a live cell,if influenced any one such function,the mutation would be lethal mutation,so that it is difficult to get berberine resistant cells.The results in this paper also prefigure that berberine and its related Chinese medicines would provide a feasible way to control antibiotic resistance problem. | JIN Jian-ling,HUA Guo-qiang,MENG Zhen,GAO Pei-ji State Key Laboratory of Microbial Technology,School of Life Sciences,Shandong University,Jinan 250100,China | 2011 | Chinese Herbal Medicines2011，3，1： | 11 |
| 16 | Sequencing of p53 mutation in established human hepatocellular carcinoma cell line of HHC4 and HHC15 in nude mice显示文摘INTRODUCTIONAlthoughtheetiopathologyofhumanhepatocelularcarcinoma(hHCC)isstilunknown,alotofevidencestronglysuggestedthatinfec... | YANG Shan Min, ZHOU Hong, CHEN Rui Chuan, WANG Yu Fang, CHEN Fu, ZHANG Chang Gong, ZHEN Yun, YAN Jiang Hua and SU Jin Hua | 1998 | World Journal of Gastroenterology1998，4，6： | 11 |
| 17 | Construction of HBV-specific ribozyme and its recombinant with HDV and their cleavage activity in vitro显示文摘AIM To construct the recombinant of HDVcDNA and HBV-specific ribozyme gene byrecombinant PCR in order to use HDV as atransporting vector carrying HBV-specificribozyme into liver cells for inhibiting thereplication of HBV.METHODS We separately cloned the ribozyme(RZ)gene and recombinant DVRZ(comprisingHDV cDNA and HBV-specific ribozyme gene)intothe downstream of T7 promoter of pTAdv-Tvector and studied the in vitro cleavage activityof their transcripts(rRZ,rDVRZ)on target RNA(rBVCF)from in vitro transcription of HBV Cgene fragment(BVCF).RESULTS Both the simple(rRZ)and therecombinant ribozyme rDVRZ could efficientlycatalyze the cleavage of target RNA(rBVCF)under different temperatures(37℃,42℃ and55℃)and Mg2+concentrations(10 mmol/L,15 mmol/L and 20 mmol/L)and their catalyticactivity tended to increase as the temperaturewas rising.But the activity of rRZ was evidentlyhigher than that of rDVRZ.CONCLUSION The recombinant of HDV cDNAand ribozyme gene had the potential of beingfurther explored and used in gene therapy of HBVinfection. | Shu Juan Wen Kai Jun Xiang Zhen Hua Huang Rong Zhou Xue Zhong Qi | 2000 | World Journal of Gastroenterology2000，6，3： | 10 |
| 18 | Transient expression and antigenic characterization of HBsAg of HBV nt551 A to G mutant显示文摘NTRODUCTIONFromthelate80s,therehavebeenincreasingnumberofreportsonhepatitisB(HB)patientswithatypicalHBVserologicalmarkers,so... | FANG De Xing 1, LI Fa Qing 1, TAN Wei Guo 1, CHEN Hua Biao 1, JIN Hui Ying 1, LI Su Qin 1, LIN Hou Ji 2 and ZHOU Zhen Xian 2 | 1999 | World Journal of Gastroenterology1999，5，1： | 10 |
| 19 | Applying the China-PAR Risk Algorithm to Assess 10-year Atherosclerotic Cardiovascular Disease Risk in Populations Receiving Routine Physical Examinations in Eastern China显示文摘Objective To assess the 10-year Atherosclerotic Cardiovascular Disease(ASCVD) risk score among adults in eastern China using the China-PAR equation which formulated primarily for the Chinese population. Methods Data from 72,129 individuals from 35-74 years old who received routine physical examinations in eastern China were analyzed in this study. The 10-year risk scores were calculated using the China-PAR equation. The chi-square test and logistic regression were then performed to evaluate the association between the selected risk factors and overall CVD risk. Results The mean 10-year ASCVD risk scores were 3.82% ± 3.76% in men and 1.30% ± 1.65% in women based on the China-PAR equation. Overall, 20% of men and 3.5% of women were intermediate-risk, and 7.3% of men and 0.3% of women were high-risk. Waist to hip ratio(WHR) [OR = 1.16(CI 95% = 1.06-1.26)], waist to height ratio(WHtR) [OR = 1.16(CI 95% = 1.05-1.28)], non-high-density lipoprotein cholesterol(non-HDL-C) [OR = 1.23(CI 95% = 1.09-1.39)], and total cholesterol(TC)/HDL-C [OR = 1.68(CI 95% = 1.46-1.94)] were more strongly associated with CVD risk than body-mass index(BMI), waist circumference(WC), and TC alone. Conclusion Male-specific prevention and treatment strategies for ASCVD are needed in eastern China. In addition, WHR, WHtR, non-HDL-C, and TC/HDL-C which not included in the the China-PAR equation were also independently associated with 10-year ASCVD risk score categories. | LI Hui Hua HUANG Shan LIU Xing Zhen ZOU Da Jin | 2019 | Biomedical and Environmental Sciences2019，32，2： | 9 |
| 20 | Optimization of the Assembly Efficiency for Lidamycin Chromophore Bound to its Apoprotein:A Case Study using Orthogonal Array显示文摘Objective Lidamycin (LDM) can be dissociated to an apoprotein (LDP) and an active enediyne chromophore (AE).The detached AE can reassemble with its LDP‐containing fusion protein to endow the latter with potent antitumor activity.However,the reassembly of AE with LDP is affected by several factors.Our aim was to optimize the assembly efficiency of the AE with a LDP‐containing fusion protein and investigate the influence of several factors on the assembly efficacy.Methods A method based on RP‐HPLC was developed to analyze the assembly rate,and an orthogonal experimental design L9 (3 4 ) was used to investigate the effects of temperature,assembly time,pH and molecular ratio of LDP‐containing fusion protein to AE on the assembly rate.Furthermore,the determined optimum conditions for the assembly rate of the LDP‐containing fusion protein with AE were applied and evaluated.Results A calibration curve based on the LDM micromolar concentration against the peak‐area of AE by HPLC was obtained.The order in which individual factors in the orthogonal experiment affected the assembly rate were temperature>time>pH>molar ratio of AE to protein and all were statistically significant (P<0.01).The optimal assembly conditions were temperature at 10°C,time of 12 h,pH 7.0,and the molar ratio of AE: protein of 5:1.The assembly rate of AE with a LDP‐containing fusion protein was improved by 23% after condition optimization.Conclusion The assembly rate of chromophore of lidamycin with its LDP‐containing fusion protein was improved after condition optimization by orthogonal design,and the optimal conditions described herein should prove useful for the development of this type of LDP‐containing fusion protein. | ZHONG Gen Shen GUO Xiao Fang ZHANG Sheng Hua ZHEN Yong Su | 2011 | Biomedical and Environmental Sciences2011，24，6： | 9 |
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