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| 1 | Rapid and Efficient Gene Modification in Rice and Brachypodium Using TALENs显示文摘 | Qiwei Shan Yanpeng Wang Kunling Chen Zhen Liang Jun Li Yi Zhang Kang Zhang Jinxing Liu Daniel F. Voytas Xuelian Zheng Yong Zhang Caixia Gao | 2013 | Molecular Plant2013，6，4： | 37 |
| 2 | Generation of thermosensitive male-sterile maize by targeted knockout of the ZmTMS5 gene显示文摘Maize(Zea mays L.)is one of the most important cereal crops,with a global production of 1.02 billion tons in 2013(Baldaufa et al.,2016).Heterosis is widely used to increase the productivity of maize,and the first commercial hybrid maize was introduced in the 1930s(Duvick,2001).The high-yield maize varieties used today are all hybrids,and the production of hybrid maize seed requires sterilization to prevent self-pollination.Conventional seed produc- | Jun Li Huawei Zhang Xiaomin Si Youhui Tian Kunling Chen Jinxing Liu Huabang Chen Caixia Gao | 2017 | Journal of Genetics and Genomics2017，44，9： | 14 |
| 3 | Highly efficient heritable genome editing in wheat using an RNA virus and bypassing tissue culture.显示文摘Genome editing provides novel strategies for improving plant traits,but relies on current genetic transformation and plant regeneration procedures,which can be inefficient.We have engineered a barley stripe mosaic virus(BSMV)-based sgRNA delivery vector(BSMV-sg)that is effective in performing heritable genome editing in Cas9-transgenic wheat plants.Mutated progenies were present in the next generation at frequencies ranging from 12.9%to 100%in three different wheat varieties,and 53.8%to 100%of mutants were virus-free.We also achieved multiplex mutagenesis in progeny using a pool of BSMV-sg vectors harboring different sgRNAs.Furthermore,we devised a virus-induced transgene-free editing procedure(VITF-Edit)to generate Cas9-free wheat mutants by crossing BSMV-infected Cas9-transgenic wheat pollen with wild-type wheat.Our study provides a robust,convenient and tissue culture-free approach for genome editing in wheat through virus infection. | Tingdong Li Jiacheng Hu Yu Sun Boshu Li Dingliang Zhang Wenli Li Jinxing Liu Dawei Li Caixia Gao Yongliang Zhang Yanpeng Wang | 2021 | Molecular Plant2021，14，11： | 10 |
| 4 | Tribenuron-Methyl Induces Male Sterility through Anther-Specific Inhibition of Acetolactate Synthase Leading to Autophagic Cell Death显示文摘Tribenuron 甲基(TM ) 是禁止分支链的强大的 sulfonylurea 除草剂由指向 acetolactate synthase (ALS ) 的催化子单元(CSR1 ) 的氨基酸(BCAA ) 生合成。由在低剂量的 TM 的叶的喷洒的男绝育的选择正式就职广泛地在油菜籽(Brassica 马来麝) 为混合种子生产被使用了;然而,内在的机制仍然保持未知。这里,我们 reportgreater TM 累积和在花药的 BCAA 饥饿比在在 TM 申请以后的叶子和茎。csr1-1D (aCSR1 异种) 的组成或花药特定的表示消除了花药选择的 ALS 抑制并且在油菜籽和 Arabidopsis 颠倒了导致 TM 的男无菌的显型。TM 涂抹茎实验的结果,在叶肉或韧皮部由 TM 新陈代谢基因 Bel 的指向的表示在导致 TM 的男绝育的花药和回复与很少 TM 累积的观察结合了,建议叶水花的 TM 通过叶肉和韧皮部主要对花药极运输。显微镜学和 immunoblotting 揭示了那 autophagy,在房间死亡期间导致的一个体积降级过程,在导致 TM 的男无菌的花药并且由被提高花药特定 ALS 击倒。而且,导致 TM 的花粉流产被 autophagy 禁止者 3 麻省显著地禁止。这些数据最终建议 TM 对花药极运输,导致经由花药特定的 ALS 抑制的 BCAA 饥饿并且,,在花药的 autophagic 房间死亡。 | Lun Zhao Xue Jing Li Chen Yingjun Liu Yanan Su Tingting Liu Changbin Gao Bin Yi Jing Wen Chaozhi Ma Jinxing Tu Jitao Zou Tingdong Fu Jinxiong Shen | 2015 | Molecular Plant2015，8，12： | 7 |
| 5 | Biolistic Genetic Transformation of a Wide Range of Chinese Elite Wheat(Triticum aestivum L.)Varieties显示文摘Wheat(Triticum aestivum L.)is a major staple food crop worldwide.It is economically important because it can be grown in a wide range of climates and geographic regions,and it has made an enormous contribution to the increase in global food production over the past four decades(Dixon et al.,2009).Wheat is produced on more than 18%of the arable land in the world,and is the most cultivated crop after | Kang Zhang Jinxing Liu Yi Zhang Zhimin Yang Caixia Gao | 2015 | Journal of Genetics and Genomics2015，42，1： | 4 |
| 6 | Transient expression of a TaGRF4-TaGIF1 complex stimulates wheat regeneration and improves genome editing显示文摘Genome editing is an unprecedented technological breakthrough but low plant regeneration frequencies and genotype dependence hinder its implementation for crop improvement. Here, we found that transient expression of a complex of the growth regulators TaGRF4 and TaGIF1(TaGRF4-TaGIF1) increased regeneration and genome editing frequency in wheat. When we introduced synonymous mutation in the miR396 target site of TaGRF4, the resulting complex(mTaGRF4-TaGIF1) performed better than original TaGRF4-TaGIF1. Use of m TaGRF4-TaGIF1 together with a cytosine base editor targeting TaALS resulted in 2-9-fold increases in regeneration and transgene-free genome editing in 11 elite common wheat cultivars. Therefore, m TaGRF4-TaGIF1 will undoubtedly be of great value in crop improvement and especially in commercial applications, since it greatly increased the range of cultivars available for transformation. | Fengti Qiu Sinian Xing Chenxiao Xue Jinxing Liu Kunling Chen Tuanyao Chai Caixia Gao | 2022 | Science China(Life Sciences)2022，65，4： | 4 |
| 7 | Highly transparent AION pre- ssurelessly sintered from powder synthesized by a novel carboth- ermal nitridation method显示文摘 | JINX H GAO L SUN J | 2012 | Journal of the American Ceramic Society2012，95，7： | 1 |
| 8 | Genome-wide analysis of the AP2/ERF gene family in Populus trichocarpa显示文摘 | ZHUANG J CAI B PENG R H ZHU B JINX F XUE Y GAO F FU X Y TIAN Y S ZHAO W QIAO Y S ZHANG Z XIONG A S YAO Q H | 2008 | Biochem Biophys Res Commun2008，371，： | 1 |
| 9 | The structural change of diphasic mullite gel studied by XRD and IR spectrum analysis显示文摘 | JINX H GAO L GUO J K | 2002 | Journal of the European Ceramic Society2002，22，8： | 1 |
| 10 | Microstructure and mechanical properties of SiC-mullite nanocomposite prepared by spark plasma sintering显示文摘 | GAO L JINX H KAWAOKA H | 2002 | Materials Science and Engineering2002，，334： | 1 |
| 11 | Study on Chemical Synthesis and Insecticidal Activity of Peptideκ-CTx-btg02显示文摘[Objectives]This study was conducted to investigate the chemical synthesis of peptideκ-CTx-btg02.[Methods]Linear peptideκ-CTx-btg02 was synthesized by solid phase peptide synthesis(SPPS).After oxidation and folding of the linear peptide,mass spectrometry identification and high performance liquid chromatography(HPLC)purification were performed.Then,the MTT method and insect injection method were applied to study its insecticidal activity.[Results]The peptideκ-CTx-btg02 was successfully synthesized by the SPPS method,and identified for the formation of disulfide bonds by mass spectrometry identification,and the purity after HPLC separation was greater than 95%.The MTT experiment showed that the peptideκ-CTx-btg02 could inhibit the growth of insect cells sf9,with a half effective dose of 0.13 nM.The insect injection experiment showed that the peptideκ-CTx-btg02 could effectively kill Tenebrio molitor,with a half lethal dose of 15.6 nM.The results of the electrophysiological experiment showed that 10μM peptideκ-CTx-btg02 had no blocking activity on murine acetylcholineα2β3 andα3β4.Therefore,the peptideκ-CTx-btg02 had a good inhibitory effect on the growth of insect cells,highly effective insecticidal activity and weak mammalian toxicity.[Conclusions]This study lays a foundation for the development of new,safe and efficient peptide biological insecticides. | Qin CHEN Jinxing FU Min XU Bingmiao GAO | 2021 | Agricultural Biotechnology2021，10，4： | 1 |
| 12 | Electrochemical preparation of silicon and its alloys from solid oxides in molten calcium chloride 显示文摘 | JINX B GAO P WANG D H | 2004 | Angew Chem Int Ed2004，43，6： | 1 |
| 13 | Long noncoding RNA TUG1 promotes proliferation, migration and cisplatin resistance in oral squamous cell carcinoma显示文摘Oral squamous cell carcinoma(OSCC)is a malignant tumor with a high metastasis rate and high recurrence rate,accounting for approximately 90%of oral cancers.Worldwide,there are more than 350,000 new cases each year.Approximately 50%of patients with OSCC are in an advanced stage at the time of treatment.Although there has been great progress in the diagnosis and treatment of tumors in recent years,the prognosis of OSCC has not been significantly improved.Studies have suggested that long noncoding RNAs(lncRNAs)are involved in the progression of cancer and can be used as cancer markers[1]. | Shuyan Liu Weirong Wang Lingyun Ye Chanjuan Liu Wei Xiao Jinxing Gao | 2023 | Acta Biochimica et Biophysica Sinica2023，55，8： | 0 |
| 14 | Identification and preliminary study of a novel interacting protein SCRIB with fibroblast activation protein in OSCC显示文摘Oral squamous cell carcinoma(OSCC)is a common oral malignant tumor.It can occur in the skin and mucosa,mostly in the tongue,palate,and gingiva.Lymph node metastasis usually occurs,and distal metastasis may also occur in the late stage[1].Currently,surgery is the main treatment method,combined with radiotherapy and chemotherapy,and once the timing of cancer treatment is delayed,the patient’s speech,eating and survival will be seriously threatened.Thus,exploring the pathway of OSCC and finding new thermal targets are basic problems to be solved[2]. | Shuyan Liu Pu Wang Lingyun Ye Chanjuan Liu Wei Xiao Chenyang Gao Xinyi Huang Jinxing Gao | 2023 | Acta Biochimica et Biophysica Sinica2023，55，8： | 0 |
| 15 | Preparation and Insecticidal Activity of Sea Anemone Peptide AP-GI from Aiptasia pallida显示文摘[Objectives] This study was conducted to synthesize sea anemone peptide Ap-GI and investigate its insecticidal activity. [Methods] The linear peptide Ap-GI was synthesized by solid phase peptide synthesis(SPPS), and the linear peptide was subjected to the two-step oxidative folding, mass spectrometry identification and high performance liquid chromatography(HPLC) purification. Then, the MTT method and insect injection method were used to study its insecticidal activity. [Results] The synthesized sea anemone peptide had a purity of 95%. The test results of the MTT method showed that the peptide Ap-GI had the activity of inhibiting the growth of insect cells sf9 with the median effective dose of 0.7 nM;and the test results of the injection method on yellow mealworms showed that the peptide Ap-GI had high insecticidal activity, and the median lethal dose was 16.9 nM. [Conclusions] The sea anemone peptide Ap-GI from Aiptasia pallida has a good inhibitory effect on the growth of insect cells and high-efficiency insecticidal activity, which can lay a foundation for the development of new, safe and efficient peptide biological insecticides. | Jinxing FU Yanling LIAO Xuefeidan LIU Bingmiao GAO | 2021 | Agricultural Biotechnology2021，10，1： | 0 |
| 16 | Identification of atypical T4SS effector proteins mediating bacterial defense显示文摘To remain competitive,proteobacteria use various contact-dependent weapon systems to defend against microbial com-petitors.The bacterial-killing type IV secretion system(T4SS)is one such powerful weapon.It commonly controls the killing/competition between species by secreting the lethal T4SS effector(T4E)proteins carrying conserved XVIPCD domains into competing cells.In this study,we sought knowledge to understand whether the bacterial-killing T4SS-producing bacteria encode T4E-like proteins and further explore their biological functions.To achieve this,we designed a T4E-guided approach to discover T4E-like proteins that are designated as atypical T4Es.Initially,this approach required scientists to perform simple BlastP search to identify T4E homologs that lack the XVIPCD domain in the genomes of T4SS-producing bacteria.These homologous genes were then screened in Escherichia coli to identify antibacterial candidates(atypical T4Es)and their neighboring detoxification proteins,followed by testing their gene cotranscription and validating their physical interactions.Using this approach,we did discover two atypical T4E proteins from the plant-beneficial Lysobacter enzymogenes and the phytopathogen Xanthomonas citri.We also provided substantial evidence to show that the atypical T4E protein Le1637-mediated bacterial defense in interspecies interactions between L.enzymogenes and its competitors.Therefore,the newly designed T4E-guided approach holds promise for detecting functional atypical T4E proteins in bacterial cells. | Xi Shen Zixiang Yang Zihan Li Dan Xiong Jinxing Liao Weimei He Danyu Shen Xiaolong Shao Ben Niu Yongxing He Yong-Gui Gao Guoliang Qian | 2023 | mLife2023，2，3： | 0 |
| 17 | Directed evolution of a neutrophilic and mesophilic methanol dehydrogenase based on high-throughput and accurate measurement of formaldehyde显示文摘Methanol is a promising one-carbon feedstock for biomanufacturing,which can be sustainably produced from carbon dioxide and natural gas.However,the efficiency of methanol bioconversion is limited by the poor catalytic properties of nicotinamide adenine dinucleotide(NAD^(+))-dependent methanol dehydrogenase(Mdh)that oxidizes methanol to formaldehyde.Herein,the neutrophilic and mesophilic NAD^(+)-dependent Mdh from Bacillus stearothermophilus DSM 2334(Mdh_(Bs))was subjected to directed evolution for enhancing the catalytic activity.The combination of formaldehyde biosensor and Nash assay allowed high-throughput and accurate measurement of formaldehyde and facilitated efficient selection of desired variants.Mdh_(Bs)variants with up to 6.5-fold higher K_(cat)/K_(M)value for methanol were screened from random mutation libraries.The T153 residue that is spatially proximal to the substrate binding pocket has significant influence on enzyme activity.The beneficial T153P mutation changes the interaction network of this residue and breaks theα-helix important for substrate binding into two shortα-helices.Reconstructing the interaction network of T153 with surrounding residues may represent a promising strategy to further improve Mdh_(Bs),and this study provides an efficient strategy for directed evolution of Mdh. | Jin Qian Liwen Fan Jinxing Yang Jinhui Feng Ning Gao Guimin Cheng Wei Pu Wenjuan Zhou Tao Cai Shuang Li Ping Zheng Jibin Sun Depei Wang Yu Wang | 2023 | Synthetic and Systems Biotechnology2023，8，3： | 0 |
| 18 | New insights into the formation of ammonium nitrate from a physical and chemical level perspective显示文摘High levels of fine particulate matter(PM_(2.5))is linked to poor air quality and premature deaths,so haze pollution deserves the attention of the world.As abundant inorganic components in PM_(2.5),ammonium nitrate(NH_(4)NO_(3))formation includes two processes,the diffusion process(molecule of ammonia and nitric acid move from gas phase to liquid phase)and the ionization process(subsequent dissociation to form ions).In this study,we discuss the impact of meteorological factors,emission sources,and gaseous precursors on NH4NO3 formation based on thermodynamic theory,and identify the dominant factors during clean periods and haze periods.Results show that aerosol liquid water content has a more significant effect on ammonium nitrate formation regardless of the severity of pollution.The dust source is dominant emission source in clean periods;while a combination of coal combustion and vehicle exhaust sources is more important in haze periods.And the control of ammonia emission is more effective in reducing the formation of ammonium nitrate.The findings of this work inform the design of effective strategies to control particulate matter pollution. | Yuting Wei Xiao Tian Junbo Huang Zaihua Wang Bo Huang Jinxing Liu Jie Gao Danni Liang Haofei Yu Yinchang Feng Guoliang Shi | 2023 | Frontiers of Environmental Science & Engineering2023，17，11： | 0 |
| 19 | Preparation of Sea Anemone Peptide Toxin Ap-TxI and Its Insecticidal Activity显示文摘[Objectives]This study was conducted to synthesize sea anemone peptide toxin Ap-TxI and investigate its insecticidal activity. [Methods] The sea anemone linear peptide toxin Ap-TxI was synthesized by the solid-phase peptide synthesis(SPPS), and six cysteines were oxidized to form three disulfide bonds by a three-step directional oxidation method. Then, purification by high performance liquid chromatography(HPLC) and mass spectrometry identification were performed. Finally, the insect cytotoxicity and insecticidal activity of Ap-TxI were studied by the MTT method and insect injection method. [Results] The oxidized peptide Ap-TxI with three disulfide bonds in natural configuration was successfully synthesized by the SPPS method, and its purity was >90% by HPLC analysis. The results of the MTT method showed that Ap-TxI was active on the growth of insect cells sf9, with a half effective dose of 0.2 nM;and the results of the mealworm injection test showed that the polypeptide Ap-TxI had high insecticidal activity with a median lethal dose of 11.7 nM. [Conclusions] The sea anemone peptide toxin Ap-TxI with high insecticidal effect was obtained, laying a foundation for the development of new, efficient and safe biological insecticides. | Yongyi XU Yanling LIAO Qiqi GUO Ming LI Jinxing FU Bingmiao GAO | 2022 | Agricultural Biotechnology2022，11，5： | 0 |
| 20 | Studies on Extraction and Activity of Peptides from Haemadipsa hainana显示文摘[Objectives]Peptides was extracted from Haemadipsa hainana and its activity was studied.[Methods]Electric stimulation,water extraction and ultrasonic extraction were used to extract the peptides from H.hainana.Then the protein content and molecular weight distribution of H.hainana peptides were detected by the BCA method and SDS-PAGE method,respectively.The antithrombin activity and analgesic activity of the three peptide extracts of H.hainana were detected by Markwardt thrombin titration method and mouse hot plate experiment,respectively.[Results]There extraction methods of electric stimulation,water extraction and ultrasonic extraction were used to extract the peptide extract of H.hainana,and the yields were as follows:water extraction>electrical stimulation>ultrasonic extraction.The three peptide extracts from H.hainana had antithrombin activity,and the antithrombin activity was as follows:water extraction>ultrasonic extraction>electrical stimulation.Through the hot plate experiment in mice,it was verified that the three peptide extracts of H.hainana had analgesic activity,and the analgesic activity was water extraction>electric stimulation>ultrasonic extraction.The analgesic activity of high-dose(100 mg/kg)group of H.hainana obtained by water extraction was slightly weaker than that of tramadol.[Conclusions]This study confirmed that the peptide extract of H.hainana had certain antithrombin and analgesic activity,laying a foundation for the subsequent development and application of H.hainana. | Lizi SHEN Yingchen YANG Jinxing FU Yanling LIAO Bingmiao GAO | 2023 | Agricultural Biotechnology2023，12，3： | 0 |
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