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| 1 | Downstaging and resection of hepatocellular carcinoma in patients with extrahepatic metastases after stereotactic therapy显示文摘Background:A combination of tyrosine kinase inhibitors(TKIs)and anti-PD-1 antibodies with local regional therapy has elicited yield substantial clinical benefits in patients who have hepatocellular carcinoma(HCC)with extrahepatic metastases.Using this treatment strategy to convert HCC patients with extrahepatic metastases from unresectable to resectable has not yet been reported.Methods:Consecutive hepatocellular carcinoma patients with extrahepatic metastases who received first-line therapy with a combination of TKIs and anti-PD-1 antibodies and at least one local regional therapy were analysed.Results:Nine patients with localized disease who received first-line systemic therapy were enrolled.At baseline,all of them had oligometastatic disease,namely,Barcelona Clinic Liver Cancer stage C(or Chinese Liver Cancer stage IIIB).The most common treatment administered was lenvatinib plus anti-PD-1 antibody and transarterial chemoembolization,and the median time span from systemic therapy to surgery was 3.2(IQR,2.8-6.2)months.Three patients achieved a pathological complete response.Six patients underwent laparoscopic surgery,and the other 3 patients underwent open surgery.After a median follow-up of 10.2(IQR,8.6-20.0)months,7 patients survived without disease recurrence,and 2 experienced tumour recurrence.All patients had any-grade AEs,and 55.6%of the patients experienced grade 3 AEs.Fatigue was the most common AE,followed by elevated aminotransferase levels and hypertension.Conclusions:Stereotactic therapy is a feasible conversion therapy for HCC patients with extrahepatic metastases to become resectable.This is the first study to analyse therapeutic outcomes of patients receiving these therapies for HCC with extrahepatic metastases. | Xiaobo Yang Haifeng Xu Bangyou Zuo Xu Yang Jin Bian Junyu Long Dongxu Wang Junwei Zhang Cong Ning Yanyu Wang Ziyu Xun Yunchao Wang Xin Lu Yilei Mao Xinting Sang Haitao Zhao | 2021 | Hepatobiliary Surgery and Nutrition2021，10，4： | 17 |
| 2 | Humoral immune response to circulating SARS-CoV-2 variants elicited by inactivated and RBD-subunit vaccines显示文摘SARS-CoV-2 variants could induce immune escape by mutations on the receptor-binding domain(RBD)and N-terminal domain(NTD).Here we report the humoral immune response to circulating SARS-CoV-2 variants,such as 501Y.V2(B.1.351),of the plasma and neutralizing antibodies(NAbs)elicited by CoronaVac(inactivated vaccine),ZF2001(RBD-subunit vaccine)and natural infection.Among 86 potent NAbs identified by high-throughput single-cell VDJ sequencing of peripheral blood mononuclear cells from vaccinees and convalescents,near half anti-RBD NAbs showed major neutralization reductions against the K417N/E484K/N501Y mutation combination,with E484K being the dominant cause.VH3-53/VH3-66 recurrent antibodies respond differently to RBD variants,and K417N compromises the majority of neutralizing activity through reduced polar contacts with complementarity determining regions.In contrast,the 242–244 deletion(242–244Δ)would abolish most neutralization activity of anti-NTD NAbs by interrupting the conformation of NTD antigenic supersite,indicating a much less diversity of anti-NTD NAbs than anti-RBD NAbs.Plasma of convalescents and CoronaVac vaccinees displayed comparable neutralization reductions against pseudo-and authentic 501Y.V2 variants,mainly caused by E484K/N501Y and 242–244Δ,with the effects being additive.Importantly,RBD-subunit vaccinees exhibit markedly higher tolerance to 501Y.V2 than convalescents,since the elicited anti-RBD NAbs display a high diversity and are unaffected by NTD mutations.Moreover,an extended gap between the third and second doses of ZF2001 leads to better neutralizing activity and tolerance to 501Y.V2 than the standard three-dose administration.Together,these results suggest that the deployment of RBD-vaccines,through a third-dose boost,may be ideal for combating SARS-CoV-2 variants when necessary,especially for those carrying mutations that disrupt the NTD supersite. | Yunlong Cao Ayijiang Yisimayi Yali Bai Weijin Huang Xiaofeng Li Zhiying Zhang Tianjiao Yuan Ran An Jing Wang Tianhe Xiao Shuo Du Wenping Ma Liyang Song Yongzheng Li Xiang Li Weiliang Song Jiajing Wu Shuo Liu Xuemei Li Yonghong Zhang Bin Su Xianghua Guo Yangyang Wei Chuanping Gao Nana Zhang Yifei Zhang Yang Dou Xiaoyu Xu Rui Shi Bai Lu Ronghua Jin Yingmin Ma Chengfeng Qin Youchun Wang Yingmei Feng Junyu Xiao Xiaoliang Sunney Xie | 2021 | Cell Research2021，31，7： | 12 |
| 3 | Polysaccharide,proline,and anti-oxidation enzyme activities of Thailand rosewood(Dalbergia cochinchinensis)seedlings exposed to exponential fertilization显示文摘Thailand rosewood(Dalbergia cochinchinensis)is a vulnerable high value tree species in subtropical and tropical Southeast Asia.The demand for the rehabilitation of the natural habitat of the species requires high-quality seedlings.However,current fertilizer regimes tend to deliver a coarse rate of fertilizers that result in a low uptake efficiency and a high risk of nutrient excess.Exponential fertilization is an effective approach to promote seedling quality through identifying an optimum dose out of a range of applications.In this study,Thailand rosewood seedlings were cultured in a mixed substrate of perlite,vermiculite,and peat(2:2:3,v/v/v)and received exponential fertilization from 0(control),through 100,200,300,400,and 600 mg nitrogen(N)per plant over a four-month cultural rotation.Height,diameter,and shoot biomass increased with fertilizer rate and were maximized at 300 mg N per plant.This application also reduced nutrient deficiencies and avoided excess supply compared to higher rates.Polysaccharide,proline,and anti-oxidation enzyme activities tended to be greater in unfertilized controls or in over-fertilized high dosages,with lower activities around 100–300 mg N plant(-1).Application of 300 mg N per plant was identified as the optimum dosage as it maximized nutrient reserves,polysaccharide and proline contents,and anti-oxidants without causing any symptoms from excess fertilizer toxicity.Future work is required to identify the effects of N-fixation on the formation of nutritional reserves in Thailand rosewood seedlings subjected to exponential fertilization. | Zhou Hong Junyu Guo Ningnan Zhang Zengjiang Yang Xiaojing Liu Zhiyi Cui Daping Xu | 2022 | Journal of Forestry Research2022，33，1： | 4 |
| 4 | HMGB1 from Astrocytes Promotes EAE by Influencing the Immune Cell Infiltration-Associated Functions of BMECs in Mice显示文摘High mobility group box 1(HMGB1)has been reported to play an important role in experimental autoimmune encephalomyelitis(EAE).Astrocytes are important components of neurovascular units and tightly appose the endothelial cells of microvessels by their perivascular endfeet and directly regulate the functions of the blood-brain barrier.Astrocytes express more HMGB1 during EAE while the exact roles of astrocytic HMGB1 in EAE have not been well elucidated.Here,using conditional-knockout mice,we found that astrocytic HMGB1 depletion decreased morbidity,delayed the onset time,and reduced the disease score and demyelination of EAE.Meanwhile,there were fewer immune cells,especially pathogenic T cells infiltration in the central nervous system of astrocytic HMGB1 conditional-knockout EAE mice,accompanied by up-regulated expression of the tight-junction protein Claudin5 and down-regulated expression of the cell adhesion molecules ICAM1 and VCAM1 in vivo.In vitro,HMGB1 released from astrocytes decreased Claudin5 while increased ICAM1 and VCAM1 expressed by brain microvascular endothelial cells(BMECs)through TLR4 or RAGE.Taken together,our results demonstrate that HMGB1 derived from astrocytes aggravates EAE by directly influencing the immune cell infiltration-associated functions of BMECs. | Junyu Shi Yifan Xiao Na Zhang Mengya Jiao Xuhuan Tang Chan Dai Chenchen Wang Yong Xu Zheng Tan Feili Gong Fang Zheng | 2022 | Neuroscience Bulletin2022，38，11： | 3 |
| 5 | Neuroligins Differentially Mediate Subtype-Specific Synapse Formation in Pyramidal Neurons and Interneurons显示文摘Neuroligins(NLs) are postsynaptic cell-adhesion proteins that play important roles in synapse formation and the excitatory-inhibitory balance. They have been associated with autism in both human genetic and animal model studies, and affect synaptic connections and synaptic plasticity in several brain regions. Yet current research mainly focuses on pyramidal neurons, while the function of NLs in interneurons remains to be understood. To explore the functional difference among NLs in the subtypespecific synapse formation of both pyramidal neurons and interneurons, we performed viral-mediated shRNA knockdown of NLs in cultured rat cortical neurons and examined the synapses in the two major types of neurons. Our results showed that in both types of neurons, NL1 and NL3 were involved in excitatory synapse formation, and NL2 in GABAergic synapse formation. Interestingly, NL1 affectedGABAergic synapse formation more specifically than NL3,and NL2 affected excitatory synapse density preferentially in pyramidal neurons. In summary, our results demonstrated that different NLs play distinct roles in regulating the development and balance of excitatory and inhibitory synapses in pyramidal neurons and interneurons. | Qiang-Qiang Xia Jing Xu Tai-Lin Liao Jie Yu Lei Shi Jun Xia Jian-Hong Luo Junyu Xu | 2019 | Neuroscience Bulletin2019，35，3： | 2 |
| 6 | Numerical Simulation of Water and Sand Blowouts When Penetrating Through Shallow Water Flow Formations in Deep Water Drilling显示文摘In this study, we applied a two-phase flow model to simulate water and sand blowout processes when penetrating shallow water flow(SWF) formations during deepwater drilling. We define ‘sand' as a pseudo-component with high density and viscosity, which can begin to flow with water when a critical pressure difference is attained. We calculated the water and sand blowout rates and analyzed the influencing factors from them, including overpressure of the SWF formation, as well as its zone size, porosity and permeability, and drilling speed(penetration rate). The obtained data can be used for the quantitative assessment of the potential severity of SWF hazards. The results indicate that overpressure of the SWF formation and its zone size have significant effects on SWF blowout. A 10% increase in the SWF formation overpressure can result in a more than 90% increase in the cumulative water blowout and a 150% increase in the sand blowout when a typical SWF sediment is drilled. Along with the conventional methods of well flow and pressure control, chemical plugging, and the application of multi-layer casing, water and sand blowouts can be effectively reduced by increasing the penetration rate. As such, increasing the penetration rate can be a useful measure for controlling SWF hazards during deepwater drilling. | REN Shaoran LIU Yanmin GONG Zhiwu YUAN Yujie YU Lu WANG Yanyong XU Yan DENG Junyu | 2018 | Journal of Ocean University of China2018，17，1： | 2 |
| 7 | Fast CO_(2) Retrieval Using a Semi-Physical Statistical Model for the High-Resolution Spectrometer on the Fengyun-3D Satellite显示文摘China’s Fengyun-3D meteorological satellite launched in December 2016 carries the high-resolution greenhouse-gases absorption spectrometer(GAS)aimed at providing global observations of carbon dioxide(CO_(2)).To date,GAS is one of the few instruments measuring CO_(2) from the near-infrared spectrum.On orbit,the oxygen(O_(2))A band suffers a disturbance,and the signal-to-noise ratio(SNR)is significantly lower than the nominal specification.This leads to difficulties in the retrieval of surface pressure and hence a degradation of the retrieval of the column-averaged CO_(2) dry air mole fraction(XCO_(2))if a full physics retrieval algorithm is used.Thus,a fast CO_(2) inverse method,named semi-physical statistical algorithm,was developed to overcome this deficiency.The instrument characteristics,the semi-physical statistical algorithm,and the results of comparison with ground-based measurements over land were introduced in this paper.XCO_(2) can be obtained from three bands,namely,the O_(2) A,weak CO_(2),and strong CO_(2) bands,with compensation from the Medium Resolution Spectral Imager-2(MERSI-2)products,ECMWF Reanaly-sis v5(ERA-5)data,and Total Carbon Column Observing Network(TCCON)data.The eigenvectors of covariance matrices and the least square fits were used to derive retrieval coefficients and yield cloud-free solutions.In addition to the GAS radiance,some key factors necessary for the accurate estimations of XCO_(2) were also taken as input information(e.g.,air mass,surface pressure,and a priori XCO_(2)).The global GAS XCO_(2) restricted over land was compared against the simultaneously collocated observations from TCCON.The retrieval algorithm can mitigate the issue caused by the low SNR of the O_(2) A band to a certain extent.Overall,through site-by-site comparisons,GAS XCO_(2) agreed well with the average precision(1σ)of 1.52 ppm and bias of−0.007 ppm.The seasonal variation trends of GAS XCO_(2) can be clearly seen at TCCON sites on the 1-yr timescale. | Yanmeng BI Peng ZHANG Zhongdong YANG Qian WANG Xingying ZHANG Chengbao LIU Pengmei XU Lizhou HOU Junyu KE Naiqiang ZHANG | 2022 | Journal of Meteorological Research2022，36，2： | 2 |
| 8 | The influence of genetic polymorphisms in drug metabolism enzymes and transporters on the pharmacokinetics of different fluvastatin formulations显示文摘The purpose of the present study was to investigate the impact of genetic polymorphism on fluvastatin pharmacokinetics.In addition,we compared the fluvastatin pharmacokinetics differences between extended-release(ER)80 mg tablet and immediate-release(IR)40 mg capsule in terms of drug metabolism enzyme and transporter genetic polymorphisms.In this open-label,randomized,two-period,two-treatment,crossover study(n=24),effects of ABCG2,SLCO1B1,ABCB1,CYP2C9 and CYP3A5 polymorphisms on the pharmacokinetics of fluvastatin were analyzed.The administration dosage for IR 40 mg and ER 80 mg were twice and once daily,respectively,for total 7 d.Blood samples for pharmacokinetic evaluation were taken on the 1st and 7th d.The lower exposure following ER was observed.For ER tablets,SLCO1B1 T521C genotype correlated with AUC 0-24 of repeat doses(P=0.010).SLCO1B1 T521C genotype had no statistically significant effect on AUC 0-24 of IR capsule of fluvastatin after single or repeated doses.In vitro study demonstrated that when the concentration of fluvastatin was low(<1μmol/l),the uptake of fluvastatin in the HEK293-OATP1B1 with SLCO1B1521TT(K m=0.18μmol/l)was faster than that with SLCO1B1521CC(K m=0.49μmol/l),On the other hand,when concentration reached to higher level(>1μmol/l),transport velocity of fluvastatin by HEK293-OATP1B1 with SLCO1B1521TT(K m=11.4μmol/l)and with SLCO1B1521TCC(K m=15.1μmol/l)tend to be the same.It suggests that the increased effect of SLCO1B1 T521C genotype on ER formulation of fluvastatin was mainly caused by lower blood concentrations.We recommend that formulation should be incorporated into future pharmacogenomics studies. | Qian Xiang Weidang Wu Nan Zhao Chuan Li Junyu Xu Lingyue Ma Xiaodan Zhang Qiufen Xie Zhuo Zhang Jiancheng Wang Weiren Xu Xia Zhao Yimin Cui | 2020 | Asian Journal of Pharmaceutical Sciences2020，15，2： | 1 |
| 9 | Genome-wide CRISPR activation screen identifies candidate receptors for SARS-CoV-2 entry显示文摘The outbreak of coronavirus disease 2019(COVID-19) caused by SARS-CoV-2 has created a global health crisis. SARS-CoV-2 infects varieties of tissues where the known receptor ACE2 is low or almost absent, suggesting the existence of alternative viral entry pathways. Here, we performed a genome-wide barcoded-CRISPRa screen to identify novel host factors that enable SARS-CoV-2 infection. Beyond known host proteins, i.e., ACE2, TMPRSS2, and NRP1, we identified multiple host components,among which LDLRAD3, TMEM30A, and CLEC4G were confirmed as functional receptors for SARS-CoV-2. All these membrane proteins bind directly to spike’s N-terminal domain(NTD). Their essential and physiological roles have been confirmed in either neuron or liver cells. In particular, LDLRAD3 and CLEC4G mediate SARS-CoV-2 entry and infection in an ACE2-independent fashion. The identification of the novel receptors and entry mechanisms could advance our understanding of the multiorgan tropism of SARS-CoV-2, and may shed light on the development of COVID-19 countermeasures. | Shiyou Zhu Ying Liu Zhuo Zhou Zhiying Zhang Xia Xiao Zhiheng Liu Ang Chen Xiaojing Dong Feng Tian Shihua Chen Yiyuan Xu Chunhui Wang Qiheng Li Xuran Niu Qian Pan Shuo Du Junyu Xiao Jianwei Wang Wensheng Wei | 2022 | Science China(Life Sciences)2022，65，4： | 1 |
| 10 | Consensus on potential biomarkers developed for use in clinical tests for schizophrenia显示文摘Background Schizophrenia is a serious mental illness affecting approximately 20 million individuals globally.Both genetic and environmental factors contribute to the illness.If left undiagnosed and untreated,schizophrenia results in impaired social function,repeated hospital admissions,reduced quality of life and decreased life expectancy.Clinical diagnosis largely relies on subjective evidence,including self-reported experiences,and reported behavioural abnormalities followed by psychiatric evaluation.In addition,psychoses may occur along with other conditions,and the symptoms are often episodic and transient,posing a significant challenge to the precision of diagnosis.Therefore,objective,specific tests using biomarkers are urgently needed for differential diagnosis of schizophrenia in clinical practice.Aims We aimed to provide evidence-based and consensus-based recommendations,with a summary of laboratory measurements that could potentially be used as biomarkers for schizophrenia,and to discuss directions for future research.Methods We searched publications within the last 10 years with the following keywords:‘schizophrenia’,‘gene’,‘inflammation’,‘neurotransmitter’,‘protein marker’,‘gut microbiota’,‘pharmacogenomics’and‘biomarker’.A draft of the consensus was discussed and agreed on by all authors at a round table session.Results We summarised the characteristics of candidate diagnostic markers for schizophrenia,including genetic,inflammatory,neurotransmitter,peripheral protein,pharmacogenomic and gut microbiota markers.We also proposed a novel laboratory process for diagnosing schizophrenia in clinical practice based on the evidence summarised in this paper.Conclusions Further efforts are needed to identify schizophrenia-specific genetic and epigenetic markers for precise diagnosis,differential diagnosis and ethnicity-specific markers for the Chinese population.The development of novel laboratory techniques is making it possible to use these biomarkers clinically to diagnose disease. | Ping Lin Junyu Sun Xiaoyan Lou Dan Li Yun Shi Zhenhua Li Peijun Ma Ping Li Shuzi Chen Weifeng Jin Shuai Liu Qing Chen Qiong Gao Lili Zhu Jie Xu Mengyuan Zhu Mengxia Wang Kangyi Liang Ling Zhao Huabin Xu Ke Dong Qingtian Li Xunjia Cheng Jinghong Chen Xiaokui Guo | 2022 | General Psychiatry2022，35，1： | 1 |
| 11 | Combine industrial and domestic wastewater treatment by periodic alloca- ting water hybrid hydrolysis acidification reactor followed by SBR显示文摘 | Bai Junyue Xu Haolong Zhang Yidan | 2013 | Biochemi- cal Engineering Journal2013，70，5： | 1 |
| 12 | Anion-regulation engineering toward Cu/In/MOF bimetallic electrocatalysts for selective electrochemical reduction of CO_(2) to CO/formate显示文摘The conversion of carbon dioxide(CO_(2))into high-value added energy fuels and chemicals(CO,formate,C_(2)H_(4),etc.)through electrochemical reduction(eCO_(2)R)is a promising avenue to sustainable development.However,low selectivity,barren activity and poor stability of the electrodes hinder the large-scale application of eCO_(2)R.Herein,we reported a copper-indium-organic-framework(CuIn-MOF)based high-performance catalyst for eCO_(2)R.Elec-trochemical measurement results reveal that CuIn-MOF exhibits high Faradaic efficiency(FE)of CO and formate(300 mV,FE_(CO)=78.6%at-0.86 V vs.RHE,FE_(HCOO^(-))=48.4%at-1.16 V vs.RHE,respectively)in a broad range of current density(20.1–88.4 mA cm^(-2))with long-term stability(6 h)for eCO_(2)R in 0.5 M KHCO_(3)electrolyte solution.Specifically,through anion-regulation engineering,SO_(4)^(2-)anion precursor is more beneficial for the formic acid generation than NO_(3)^(-)anion precursor;while for SO_(4)^(2-)anion precursor,Cu plays a positive regulating role in eCO_(2)R to CO compared to In.Additionally,the high performance in a home-made eCO_(2)R reactor derives benefit from enhanced intrinsic activity and charge re-distribution can be attributed to the formation of In-doped Cu layer. | Bingqing Xu Israr Masood Ul Hasan Luwei Peng Junyu Liu Nengneng Xu Mengyang Fan Nabeel Khan Niazi Jinli Qiao | 2022 | Materials Reports(Energy)2022，2，3： | 1 |
| 13 | Calcium carbonate promotes the formation and stability of soil macroaggregates in mining areas of China显示文摘We studied changes in the concentrations of aggregate-cementing agents after different reclamation times and with different fertilization regimes,as well as the formation mechanism of aggregates in reclaimed soil,to provide a theoretical basis for rapid reclamation of soil fertility in the subsidence area of coal mines in Shanxi Province,China.In this study,soil samples of 0–20 cm depth were collected from four fertilization treatments of a longterm experiment started in 2008:no fertilizer (CK),inorganic fertilizer (NPK),chicken manure compost (M),and50%inorganic fertilizer plus 50%chicken manure compost (MNPK).The concentrations of cementing agents and changes in soil aggregate size distribution and stability were analysed.The results showed that the formation of>2 mm aggregates,the aggregate mean weight diameter (MWD),and the proportion of>0.25 mm water-stable aggregates (WR_(0.25)) increased significantly after 6 and 11 years of reclamation.The concentration of organic cementing agents tended to increase with reclamation time,whereas free iron oxide (Fed) and free aluminium oxide(Ald) concentrations initially increased but then decreased.In general,the MNPK treatment signi?cantly increased the concentrations of organic cementing agents and CaCO_(3),and CaCO_(3) increased by 60.4%at 11 years after reclamation.Additionally,CaCO_(3) had the greatest effect on the stability of aggregates,promoting the formation of>0.25 mm aggregates and accounting for 54.4%of the variance in the proportion and stability of the aggregates.It was concluded that long-term reclamation is bene?cial for improving soil structure.The MNPK treatment was the most effective measure for increasing maize grain yield and concentration of organic cementing agents and CaCO_(3). | Junyu Xie Jianyong Gao Hanbing Cao Jiahui Li Xiang Wang Jie Zhang Huisheng Meng Jianping Hong Tingliang Li Minggang Xu | 2024 | Journal of Integrative Agriculture2024，23，3： | 0 |
| 14 | Multiple-Channel Weight-Based CNN Fault Diagnosis Method显示文摘It is difficult to comprehensively extract device status information for CNNs under a single source high-frequency timing signal,and CNNs cannot effec-tively achieve precise identification and classification based on the importance of multichannel features.This article proposes a CNN fault diagnosis method based on multi-channel weight adaptation.This methodfirst normalizes different data sources as input as different channels of CNN,and uses the characteristics of convolutional networks to achieve the characteristics of different data sources.Fusion and extraction.Then,the SNET module is embedded into the CNN net-work,adapted to the weight of each channel,and the accuracy of classification is improved.Finally,through comparative experiments,this method can further improve the accuracy of fault recognition. | Peng Xu Xinyu Liu Junyu Lin Zhongyu Lu Fengming Li Husheng Gou | 2023 | 国际计算机前沿大会会议论文集2023，，1： | 0 |
| 15 | Dynamic phasor-based hybrid simulation for multi-inverter grid-connected system显示文摘To realize the efficient transient simulation of a grid-connected power generation system based on multiple inverters, this paper proposes a hybrid simulation method integrating the models of electromagnetic transient and dynamic phasors. Based on a demonstration of the concepts and properties of dynamic phasors, the models of single-phase and three-phase inverters described by dynamic phasors are established first. Considering the numerical compatibility problem between dynamic phasors and instantaneous values, an interface scheme between dynamic phasors and instantaneous values is designed, and the efficiency and precision differences of various transformation methods are compared in detail.Finally, by utilizing MATLAB/Simulink, a hybrid simulation platform of a multi-inverter grid-connected system is built, and the efficiency and accuracy of the hybrid simulation are validated via comparison with the full electromagnetic transient simulation. | Peng Yang Qian Li Junyu Zhao Juanjuan Lu Yunfei Xu | 2023 | Global Energy Interconnection2023，6，2： | 0 |
| 16 | Crystallography of low Z material at ultrahigh pressure:Case study on solid hydrogen显示文摘Diamond anvil cell techniques have been improved to allow access to the multimegabar ultrahigh-pressure region for exploring novel phenomena in condensedmatter.However,the onlyway to determine crystal structures of materials above 100 GPa,namely,X-ray diffraction(XRD),especially for lowZ materials,remains nontrivial in the ultrahigh-pressure region,even with the availability of brilliant synchrotron X-ray sources.In thiswork,we performa systematic study,choosing hydrogen(the lowest X-ray scatterer)as the subject,to understand how to better perform XRD measurements of low Z materials at multimegabar pressures.The techniques that we have developed have been proved to be effective in measuring the crystal structure of solid hydrogen up to 254GPa at room temperature[C.Ji et al.,Nature 573,558–562(2019)].Wepresent our discoveries and experienceswith regard to several aspects of thiswork,namely,diamond anvil selection,sample configuration for ultrahigh-pressure XRDstudies,XRDdiagnostics for low Z materials,and related issues in data interpretation and pressure calibration.Webelieve that these methods can be readily extended to other low Z materials and can pave the way for studying the crystal structure of hydrogen at higher pressures,eventually testing structural models of metallic hydrogen. | Cheng Ji Bing Li Wenjun Liu Jesse S.Smith Alexander Bjoorling Arnab Majumdar Wei Luo Rajeev Ahuja Jinfu Shu Junyue Wang Stanislav Sinogeikin Yue Meng Vitali B.Prakapenka Eran Greenberg Ruqing Xu Xianrong Huang Yang Ding Alexander Soldatov Wenge Yang Guoyin Shen Wendy L.Mao Ho-Kwang Mao | 2020 | Matter and Radiation at Extremes2020，5，3： | 0 |
| 17 | Functional characterization of novel NPRL3 mutations identified in three families with focal epilepsy显示文摘Focal epilepsy accounts for 60% of all forms of epilepsy, but the pathogenic mechanism is not well understood. In this study,three novel mutations in NPRL3(nitrogen permease regulator-like 3), c.937_945del, c.1514dup C and 6,706-bp genomic DNA(g DNA) deletion, were identified in three families with focal epilepsy by linkage analysis, whole exome sequencing(WES) and Sanger sequencing. NPRL3 protein is a component of the GATOR1 complex, a major inhibitor of m TOR signaling. These mutations led to truncation of the NPRL3 protein and hampered the binding between NPRL3 and DEPDC5, which is another component of the GATOR1 complex. Consequently, the mutant proteins enhanced m TOR signaling in cultured cells, possibly due to impaired inhibition of m TORC1 by GATOR1. Knockdown of nprl3 in Drosophila resulted in epilepsy-like behavior and abnormal synaptic development. Taken together, these findings expand the genotypic spectrum of NPRL3-associated focal epilepsy and provide further insight into how NPRL3 mutations lead to epilepsy. | Shiyue Du Sheng Zeng Li Song Hongying Ma Rui Chen Junyu Luo Xu Wang Tingbin Ma Xuan Xu Hao Sun Ping Yi Jifeng Guo Yaling Huang Mugen Liu Tao Wang Wei-Ping Liao Luoying Zhang Jing Yu Liu Beisha Tang | 2023 | Science China(Life Sciences)2023，66，9： | 0 |
| 18 | Mechanisms of PiT2-loop7 Missense Mutations Induced Pi Dyshomeostasis显示文摘PiT2 is an inorganic phosphate(Pi)transporter whose mutations are linked to primary familial brain calcification(PFBC).PiT2 mainly consists of two ProDom(PD)domains and a large intracellular loop region(loop7).The PD domains are crucial for the Pi transport,but the role of PiT2-loop7 remains unclear.In PFBC patients,mutations in PiT2-loop7 are mainly nonsense or frameshift mutations that probably cause PFBC due to C-PD1131 deletion.To date,six missense mutations have been identified in PiT2-loop7;however,the mechanisms by which these mutations cause PFBC are poorly understood.Here,we found that the p.T390A and p.S434W mutations in PiT2-loop7 decreased the Pi transport activity and cell surface levels of PiT2.Furthermore,we showed that these two mutations attenuated its membrane localization by affecting adenosine monophosphate-activated protein kinase(AMPK)-or protein kinase B(AKT)-mediated PiT2 phosphorylation.In contrast,the p.S121C and p.S601W mutations in the PD domains did not affect PiT2 phosphorylation but rather impaired its substrate-binding abilities.These results suggested that missense mutations in PiT2-loop7 can cause Pi dyshomeostasis by affecting the phosphorylation-regulated cell-surface localization of PiT2.This study helps understand the pathogenesis of PFBC caused by PiT2-loop7 missense mutations and indicates that increasing the phosphorylation levels of PiT2-loop7 could be a promising strategy for developing PFBC therapies. | Hao Sun Xuan Xu Junyu Luo Tingbin Ma Jiaming Cui Mugen Liu Bo Xiong Shujia Zhu Jing-Yu Liu | 2023 | Neuroscience Bulletin2023，39，1： | 0 |
| 19 | The first high spatial resolution multi-scale daily SPI and SPEI raster dataset for drought monitoring and evaluating over China from 1979 to 2018显示文摘Standardized Precipitation Index(SPI)and Standardized Precipitation Evapotranspiration Index(SPEI),traditionally derived at a monthly scale,are widely used drought indices.To overcome temporalresolution limitations,we have previously developed and published a well-validated daily SPI/SPEI in situ dataset.Although having a high temporal resolution,this in situ dataset presents low spatial resolution due to the scarcity of stations.Therefore,based on the China Meteorological Forcing Dataset,which is composed of data from more than 1,000 ground-based observation sites and multiple remote sensing grid meteorological dataset,we present the first high spatiotemporal-resolution daily SPI/SPEI raster datasets over China.It spans from 1979 to 2018,with a spatial resolution of 0.1°×0.1°,a temporal resolution of 1-day,and the timescales of 30-,90-,and 360-days.Results show that the spatial distributions of drought event characteristics detected by the daily SPI/SPEI are consistent with the monthly SPI/SPEI.The correlation between the daily value of the 12-month scale and the monthly value of SPI/SPEI is the strongest,with linear correlation,Nash-Sutcliffe coefficient,and normalized root mean square error of 0.98,0.97,and 0.04,respectively.The daily SPI/SPEI is shown to be more sensitive to flash drought than the monthly SPI/SPEI.Our improved SPI/SPEI shows high accuracy and credibility,presenting enhanced results when compared to the monthly SPI/SPEI.The total data volume is up to 150 GB,compressed to 91 GB in Network Common Data Form(NetCDF).It can be available from Figshare(http://gffzzd3cc09b8251d45dfs96xqxvcoxofk6cpq.ffgz.tsg.suse.edu.cn/10.6084/m9.figshare.c.5823533)and ScienceDB(http://gffzzd3cc09b8251d45dfs96xqxvcoxofk6cpq.ffgz.tsg.suse.edu.cn/10.57760/sciencedb.j00076.00103). | Rongrong Zhang Virgílio A.Bento Junyu Qi Feng Xu Jianjun Wu Jianxiu Qiu Jianwei Li Wei Shui Qianfeng Wang | 2023 | Big Earth Data2023，7，3： | 0 |
| 20 | 组蛋白分子伴侣FACT促进DNA复制偶联的核小体组装显示文摘真核生物DNA复制时,复制叉前的核小体解聚以允许复制体前行,同时在新合成的DNA子链上新的核小体必须马上组装,这被称为DNA复制偶联的核小体组装,是染色质复制的关键一步。 | Jiayi Yang Xu Zhang Jianxun Feng He Leng Shuqi Li Junyu Xiao Shaofeng Liu Zhiyun Xu Jiawei Xu Di Li Zhongshi Wang Jingyang Wang 李晴 | 2017 | 科学新闻2017，19，4： | 0 |
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