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| 1 | OSBPL2-disrupted pigs recapitulate dual features of human hearing loss and hypercholesterolaemia显示文摘Oxysterol binding protein like 2(OSBPL2), an important regulator in cellular lipid metabolism and transport, was identified as a novel deafness-causal gene in our previous work. To resemble the phenotypic features of OSBPL2 mutation in animal models and elucidate the potential genotypephenotype associations, the OSBPL2-disrupted Bama miniature(BM) pig model was constructed using CRISPR/Cas9-mediated gene editing, somatic cell nuclear transfer(SCNT) and embryo transplantation approaches, and then subjected to phenotypic characterization of auditory function and serum lipid profiles. The OSBPL2-disrupted pigs displayed progressive hearing loss(HL) with degeneration/apoptosis of cochlea hair cells(HCs) and morphological abnormalities in HC stereocilia, as well as hypercholesterolaemia. High-fat diet(HFD) feeding aggravated the development of HL and led to more severe hypercholesterolaemia. The dual phenotypes of progressive HL and hypercholesterolaemia resembled in OSBPL2-disrupted pigs confirmed the implication of OSBPL2 mutation in nonsydromic hearing loss(NSHL) and contributed to the potential linkage between auditory dysfunction and dyslipidaemia/hypercholesterolaemia. | Jun Yao Huasha Zeng Min Zhang Qinjun Wei Ying Wang Haiyuan Yang Yajie Lu Rongfeng Li Qiang Xiong Lining Zhang Zhibin Chen Guangqian Xing Xin Cao Yifan Dai | 2019 | Journal of Genetics and Genomics2019，46，8： | 7 |
| 2 | The effects of modulation period,modulation ratio,and deposition temperature on microstructure and mechanical properties of ZrB_2/W multilayers显示文摘Monolithic ZrB2,W coatings and ZrB2/W multilayers with different modulation periods and modulation ratios were synthesized by ion beam assisted deposition at room temperature and 400°C.X-ray diffraction (XRD),scanning electron microscopy (SEM),surface profiler,and nanoindention were employed to investigate the influences of the deposition temperature and the modulation period on the growth,textures,interface structure,and mechanical properties of the multilayers.The results indicated that the multilayer with modulation period of 13 nm synthesized at room temperature possessed a higher hardness of 23.8 GPa.Deposition temperature gave a significant contribution to mechanical property enhancement.The 400°C-deposition temperature led to a maximum hardness and elastic modulus value of 32.1 and 399.1 GPa for ZrB2/W multilayer with a modulation period of 6.7 nm.Its critical load increased to 42.8 mN and residual stress decreased to -0.7 GPa.A higher deposition temperature can cause an increase in interfacial atomic mixture and mobility of surface species,which induceds an increase in areal atomic density and dislocation pinning.These results as well as small nanoscale grain sizes should be related to hardness increase. | LIU MengYin,TAN Ming,LIU GuangQing,WANG HaiYuan,XUE FengYing,DENG XiangYun & LI DeJun College of Physics and Electronic Information Science,Tianjin Normal University,Tianjin 300387,China | 2010 | Science China(Technological Sciences)2010，53，9： | 4 |
| 3 | Cloning and expression pattern of a dehydrin-like BDNI gene from drought-tolerant Boea crassifolia Hemsl.显示文摘A 500-bp cDNA fragment was amplified via RT-PCR from drought-induced total RNA of the drought-tolerant B. crassifolia Hemsl. using primers based on the sequence of published dehydrin conserved region. By using 5’ RACE, full-length coding region (1 148 bp) of BDN1 gene was produced. It is a new member of the dehydrin gene family. Southern analysis indicated that BDN1 is present in the 6. crassifolia genome as a single-copy gene. Northern analysis revealed that its expression is inducible by drought and cold stresses as well as ABA application. | ZHAO Huiwu, LIU Han, YU Haiyuan, HU Yuanlei, GAO Yin, LI Zhenyu & LIN ZhongpingNational Laboratory of Protein Engineering and Plant Genetic Engineering , Peking University, Beijing 100871, China Botanical Institute, Chinese Academy of Sciences, Beijing 100093, China Correspondence should be addressed to Lin Zhongping | 2000 | Chinese Science Bulletin2000，45，22： | 3 |
| 4 | Antigenicity of tissues and organs from GGTA1/CMAH/β4GalNT2 triple gene knockout pigs显示文摘Clinical xenotransplantations have been hampered by human preformed antibody-mediated damage of the xenografts.To overcome biological incompatibility between pigs and humans,one strategy is to remove the major antigens[Gal,Neu5 Gc,and Sd(a)]present on pig cells and tissues.Triple gene(GGTAI,CMAH,and β4 GalNT2)knockout(TKO)pigs were produced in our laboratory by CRISPR-Cas9 targeting.To investigate the antigenicity reduction in the TKO pigs,the expression levels of these three xenoantigens in the cornea,heart,liver,spleen,lung,kidney,and pancreas tissues were examined.The level of human IgG/IgM binding to those tissues was also investigated,with wildtype pig tissues as control.The results showed that aGal,Neu5 Gc,and Sd(a)were markedly positive in all the examined tissues in wildtype pigs but barely detected in TKO pigs.Compared to wildtype pigs,the liver,spleen,and pancreas of TKO pigs showed comparable levels of human IgG and IgM binding,whereas corneas,heart,lung,and kidney of TKO pigs exhibited significantly reduced human IgG and IgM binding.These results indicate that the antigenicity of TKO pig is significantly reduced and the remaining xenoantigens on porcine tissues can be eliminated via a gene targeting approach. | Ronggen Wang Miaomiao Ruan Runjie Zhang Lei Chen Xiaoxue Li Bin Fang Chu Li Xueyang Ren Jiying Liu Qiang Xiong Lining Zhang Yong Jin Lin Li Rongfeng Li Ying Wang Haiyuan Yang Yifan Dai | 2019 | The Journal of Biomedical Research2019，33，4： | 3 |
| 5 | Overexpressing dominant-negative FGFR2-IIIb impedes lung branching morphogenesis in pigs显示文摘Genetic studies with mouse models have shown that fibroblast growth factor receptor 2-IIIb(FGFR2-IIIb)plays crucial roles in lung development and differentiation. To evaluate the effect of FGFR2-IIIb in pig lung development, we employed somatic cell nuclear transfer(SCNT) technology to generate transgenic pig fetuses overexpressing the transmembrane(dn FGFR2-IIIb-Tm) and soluble(dn FGFR2-IIIb-HFc) forms of the dominant-negative human FGFR2-IIIb driven by the human surfactant protein C(SP-C) promoter,which was specifically expressed in lung epithelia. Eight dn FGFR2-IIIb-Tm transgenic and twelve dn FGFR2-IIIb-HFc transgenic pig fetuses were collected from three and two recipient sows, respectively.Repression of FGFR2-IIIb in lung epithelia resulted in smaller lobes and retardation of alveolarization in both forms of dn FGFR2-IIIb transgenic fetuses. Moreover, the dn FGFR2-IIIb-HFc transgenic ones showed more deterioration in lung development. Our results demonstrate that disruption of FGFR2-IIIb signaling in the epithelium impedes normal branching and alveolarization in pig lungs, which is less severe than the results observed in transgenic mice. The dn FGFR2-IIIb transgenic pig is a good model for the studies of blastocyst complementation as well as the mechanisms of lung development and organogenesis. | Qin Chen Bin Fang Ying Wang Chu Li Xiaoxue Li Ronggen Wang Qiang Xiong Lining Zhang Yong Jin Manling Zhang Xiaorui Liu Lin Li Lisha Mou Rongfeng Li Haiyuan Yang Yifan Dai | 2018 | Journal of Genetics and Genomics2018，45，3： | 2 |
| 6 | A Bama miniature pig model of monoallelic TSC1 mutation for human tuberous sclerosis complex显示文摘Tuberous sclerosis complex(TSC)is a dominant genetic neurocutaneous syndrome characterized by multiple organ hamartomas.Although rodent models bearing a germline mutation in either TSC1 or TSC2 gene have been generated,they do not develop pathogenic lesions matching those seen in patients with TSC because of the significant differences between mice and humans,highlighting the need for an improved large animal model of TSC.Here,we successfully generate monoallelic TSC1-modified Bama miniature pigs using the CRISPR/Cas9 system along with somatic cell nuclear transfer(SCNT)technology.The expression of phosphorylated target ribosomal protein S6 is significantly enhanced in the piglets,indicating that disruption of a TSC1 allele activate the mechanistic target of rapamycin(mTOR)signaling pathway.Notably,differing from the mouse TSC models reported previously,the TSC1^(+/−)Bama miniature pig developed cardiac rhabdomyoma and subependymal nodules,resembling the major clinical features that occur in patients with TSC.These TSC1^(+/−)Bama miniature pigs could serve as valuable large animal models for further elucidation of the pathogenesis of TSC and the development of therapeutic strategies for TSC disease. | Xiaoxue Li Tingdong Hu Jiying Liu Bin Fang Xue Geng Qiang Xiong Lining Zhang Yong Jin Xiaorui Liu Lin Li Ying Wang Rongfeng Li Xiaochun Bai Haiyuan Yang Yifan Dai | 2020 | Journal of Genetics and Genomics2020，47，12： | 2 |
| 7 | Self-assembly control and experiments in swarm modular robots显示文摘This paper presents a self-assembly control strategy for the swarm modular robots. Simulated and physical experiments are conducted based on the Sambot platform, which is a novel self-assembly modular robot having the characteristics of both the chain-type and the mobile self-reconfigurable robots. Multiple Sambots can autonomously move and connect with one another through self-assembly to form robotic organisms. The configuration connection state table is used to describe the configuration of the robotic structure. A directional self-assembly control model is proposed to perform the self-assembly experiments. The self-assembly process begins with one Sambot as the seed, and then the Docking Sambots use a behavior-based controller to achieve connection with the seed Sambot. The controller is independent of the target configuration. The seed and connected Sambots execute a configuration comparison algorithm to control the growth of the robotic structure. Furthermore, the simul- taneous self-assembly of multiple Sambots is discussed. For multiple configurations, self-assembly experiments are conducted in simulation platform and physical platform of Sambot. The experimental results verify the effectiveness and scalability of the self-assembly algorithms. | WEI HongXing LI HaiYuan TAN JinDong WANG TianMiao | 2012 | Science China(Technological Sciences)2012，55，4： | 2 |
| 8 | Photodegradation catalyst screening by combinatorial methodology 显示文摘 | Dai Qixiu Xiao Haiyuan Li Wensheng | 2005 | Applied Catalysis:A General2005，290，12： | 1 |
| 9 | Recent Advances in 2D Lateral Heterostructures显示文摘Recent developments in synthesis and nanofabrication technologies offer the tantalizing prospect of realizing various applications from twodimensional(2D)materials.A revolutionary development is to flexibly construct many different kinds of heterostructures with a diversity of 2D materials.These 2D heterostructures play an important role in semiconductor and condensed matter physics studies and are promising candidates for new device designs in the fields of integrated circuits and quantum sciences.Theoretical and experimental studies have focused on both vertical and lateral 2D heterostructures;the lateral heterostructures are considered to be easier for planner integration and exhibit unique electronic and photoelectronic properties.In this review,we give a summary of the properties of lateral heterostructures with homogeneous junction and heterogeneous junction,where the homogeneous junctions have the same host materials and the heterogeneous junctions are combined with different materials.Afterward,we discuss the applications and experimental synthesis of lateral 2D heterostructures.Moreover,a perspective on lateral 2D heterostructures is given at the end. | Jianwei Wang Zhiqiang Li Haiyuan Chen Guangwei Deng Xiaobin Niu | 2019 | Nano-Micro Letters2019，11，3： | 1 |
| 10 | Houttuynia cordata inhibits lipopolysaccharide-induced rapid pulmonary fibrosis by up-regulating IFN-γ and inhibiting the TGF-β1/Smad pathway显示文摘 | Shaohui Du Hui Li Yinghai Cui Lina Yang Jingjing Wu Haiyuan Huang Yangyan Chen Wei Huang Rong Zhang Jun Yang Dongfeng Chen Yiwei Li Saixia Zhang Jianhong Zhou Zhijun Wei Ngai Tan Chow | 2012 | International Immunopharmacology2012，，3： | 1 |
| 11 | Crystallographic facet-dependent stress responses by polyhedral lead sulfide nanocrystals and the potential "safe-by-design" approach显示文摘nanomaterials 的特别物理化学的性质能得到唯一的生物回答。性质活动关系通常为毒性机制的深入的理解并且设计更安全的 nanomaterials 被建立。在这研究,一系列多面的铅硫化物(PbS ) 的特定的结晶的方面的有毒的角色包括截断的八面体, cuboctahedrons,截断的立方体,和立方体, nanocrystals 在人的支气管的上皮的房间(BEAS-2B ) 和鼠科的牙槽的巨噬细胞被调查(未加工 264.7 ) 房间。{ 100 }方面触发方面依赖者被发现有能力细胞的氧化应力和重金属压力回答例如谷胱甘肽弄空,类脂化合物 peroxidation ,反应的氧种类( ROS )生产, heme oxygenase-1 ( HO-1 )和 metallothionein ( MT )表示,和 mitochondrial 机能障碍,当时{ 111 }方面在生物条件下面仍然保持惰性。{ 100 }-facet-dependent 毒性被归功于到 { 100 }-facet-dependent 铅溶解,当时低铅溶解 { 111 } 方面由于负担得起由的强壮的保护 poly (乙烯基 pyrrolidone ) 在合成期间。基于这种方面毒性关系, safe-by-design 策略被设计阻止铅溶解 { 100 } 通过原子地薄的铅氯化物 adlayers 的形成的方面,导致更安全的多面的 PbS nanocrystals。 | Yun Chang Kai Li Yanlin Feng Ning Liu Yan Cheng Xiujuan Sun Yuqing Feng Xi Li Zhijian Wu Haiyuan Zhang | 2016 | Nano Research2016，9，12： | 1 |
| 12 | Evolution of wealth inequality in China显示文摘 | Shi Li Haiyuan Wan | 2015 | China Economic Journal2015，，3： | 1 |
| 13 | Preparation of ultra-broadband antireflective coatings for amplifier blast shields by a sol–gel method显示文摘A type of λ/4–λ/4 ultra-broadband antireflective coating has been developed using modified low refractive silica and high refractive silica layers by a sol–gel dip coating method for amplifier blast shields of the Shen Guang Ⅱ high power laser facility(SG-Ⅱ facility). Deposition of the first layer(high refractive index silica) involves baking at 200℃ in the post-treatment step. The second layer(low refractive index, n = 1.20) uses low refractive index silica sol modified by acid catalysis. Thermal baking at temperatures no less than 500℃ for 60 min offers chemical stability, ethanol scratch resistance, and resistance to washing with water. The average residual reflection of dual-side-coated fused silica glass was less than 1% in the spectral range from 450 to 950 nm. Transmission gain has been evaluated by taking into account angular light, and the results show that the transmission gain increases with increasing light incidence. Even at 60°, the transmission spectrum of the broadband antireflective coating effectively covered the main absorption peak of Nd:glass. | Huai Xiong Bin Shen Zhiya Chen Xu Zhang Haiyuan Li Yongxing Tang Lili Hu | 2017 | High Power Laser Science and Engineering2017，5，4： | 1 |
| 14 | A Preliminary Study on Hydrate Anti-agglomerant Applied in Multiphase Flow Pipeline显示文摘 | Haiyuan Yao Qingping Li Guangjin Chen Jing Gong | 2013 | Journal of Energy and Power Engineering2013，7，2： | 1 |
| 15 | Bismuth telluride nanostructures: preparation, thermoelectric properties and topological insulating effect显示文摘 | Eric ASHALLEY Haiyuan CHEN Xin TONG Handong LI Zhiming M. WANG | 2015 | Frontiers of Materials Science2015，9，2： | 1 |
| 16 | microRNA?106a modulates cisplatin sensitivity by targeting PDCD4 inhuman ovarian cancer cells显示文摘 | Hao Li Haiyuan Xu Huiling Shen Hao Li | 2014 | Oncology Letters2014，，： | 1 |
| 17 | A Review of Smart Materials for the Boost of Soft Actuators,Soft Sensors,and Robotics Applications显示文摘With the advance of smart material science,robotics is evolving from rigid robots to soft robots.Compared to rigid robots,soft robots can safely interact with the environment,easily navigate in unstructured fields,and be minimized to operate in narrow spaces,owning to the new actuation and sensing technologies developed by the smart materials.In the review,different actuation and sensing technologies based on different smart materials are analyzed and summarized.According to the driving or feedback signals,actuators are categorized into electrically responsive actuators,thermally responsive actuators,magnetically responsive actuators,and photoresponsive actuators;sensors are categorized into resistive sensors,capacitive sensors,magnetic sensors,and optical waveguide sensors.After introducing the principle and several robotic prototypes of some typical materials in each category of the actuators and sensors.The advantages and disadvantages of the actuators and sensors are compared based on the categories,and their potential applications in robotics are also presented. | Yufei Hao Shixin Zhang Bin Fang Fuchun Sun Huaping Liu Haiyuan Li | 2022 | Chinese Journal of Mechanical Engineering2022，35，2： | 1 |
| 18 | Multiplication of the Recombinant Strain Re-7 of Avian Influenza Virus Subtype H5 in MDCK Cells显示文摘This study was conducted to explore the multiplication pattern of the recombinant strain Re-7 of avian influenza virus subtype H5 in Madin Darby Canine Kidney(MDCK) cells and to determine the optimal multiplicity of infection(MOI) and the optimal time for virus harvest. The recombinant strain Re-7 was inoculated at different MOIs into MDCK cells grown in serum-free medium in 100 L bioreactors for replication. Then,the hemagglutination(HA) titer, 50% tissue culture infectious dose(TCID50) and 50% embryo infectious dose(EID50) of culture medium were measured once every 12 h from 24 h after virus inoculation to determine the optimal MOI. After that, virus was inoculated at the optimal MOI deter-mined above into MDCK cells for large-scale virus replication to determine the optimal time for virus harvest. The results showed that the optimal MOI was 10^(-2), and the optimal time for virus harvest was 60 h after inoculation. Under these conditions, the HA titer, TCID_(50) per 1 m L and EID50 per 0.1 m L were increased to 1∶102 4, 10^(7.33) and 10^(6.83), respectively. This study provides relatively stable parameters for large-scale production of the recombinant strain Re-7 of avian influenza virus subtype H5. | Chen Hong Wang Bo Zhao Haiyuan Li Jinxiang Zhao Bo Li Li Wang Yuhong Cui Kai Zhu Changdong | 2018 | Animal Husbandry and Feed Science2018，10，3： | 0 |
| 19 | Protective effects on acute hypoxic-ischemic brain damage in mfat-1 transgenic mice by alleviating neuroinflammation显示文摘Acute hypoxic-ischemic brain damage(HIBD)mainly occurs in adults as a result of perioperative cardiac arrest and asphyxia.The benefits of n-3 polyunsaturated fatty acids(n-3 PUFAs)in maintaining brain growth and development are well documented.However,possible protective targets and underlying mechanisms of mfat-1 mice on HIBD require further investigation.The mfat-1 transgenic mice exhibited protective effects on HIBD,as indicated by reduced infarct range and improved neurobehavioral defects.RNA-seq analysis showed that multiple pathways and targets were involved in this process,with the anti-inflammatory pathway as the most significant.This study has shown for the first time that mfat-1 has protective effects on HIBD in mice.Activation of a G protein-coupled receptor 120(GPR120)-related anti-inflammatory pathway may be associated with perioperative and postoperative complications,thus innovating clinical intervention strategy may potentially benefit patients with HIBD. | Xue Geng Meng Wang Yunjun Leng Lin Li Haiyuan Yang Yifan Dai Ying Wang | 2021 | The Journal of Biomedical Research2021，35，6： | 0 |
| 20 | An internal ballistic model of electromagnetic railgun based on PFN coupled with multi-physical field and experimental validation显示文摘To accelerate the practicality of electromagnetic railguns,it is necessary to use a combination of threedimensional numerical simulation and experiments to study the mechanism of bore damage.In this paper,a three-dimensional numerical model of the augmented railgun with four parallel unconventional rails is introduced to simulate the internal ballistic process and realize the multi-physics field coupling calculation of the rail gun,and a test experiment of a medium-caliber electromagnetic launcher powered by pulse formation network(PFN)is carried out.Various test methods such as spectrometer,fiber grating and high-speed camera are used to test several parameters such as muzzle initial velocity,transient magnetic field strength and stress-strain of rail.Combining the simulation results and experimental data,the damage condition of the contact surface is analyzed. | Benfeng Gu Haiyuan Li Baoming Li | 2024 | Defence Technology(防务技术)2024，32，2： | 0 |
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