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| 1 | Ythdc2 is an N^6-methyladenosine binding protein that regulates mammalian spermatogenesis显示文摘N 6-methyladenosine (m 6 一) 是在真核细胞的 mRNA 的最普通的内部修正。它动态地被安装并且搬迁,并且充当 mRNA 新陈代谢,包括干细胞 pluripotency 的调整生物过程,房间区别,和精力动态平衡的新层。m 6 A 被选择有约束力的蛋白质认出;YTHDF1 和 YTHDF3 在音乐会工作影响 m 6包含 A 的 mRNAs, YTHDF2 帮助 mRNA 腐烂,和 YTHDC1 影响它的目标的原子处理。YTHDC2 的生物功能, YTH 蛋白质家庭,的最后的成员仍然保持未知。我们报导 YTHDC2 有选择地绑 m 6 在它的一致主题的 A。YTHDC2 提高它的目标的翻译效率并且也减少他们的 mRNA 丰富。Ythdc2 猛烈老鼠是不肥沃的;男性们让显著地更小的睾丸和女性同窝出生的人与那些相比有显著地更小的卵巢。Ythdc2 猛烈老鼠的细菌房间不经过 zygotene 阶段发展,因此,当成熟分裂开始, Ythdc2 是在睾丸的 upregulated。因此, YTHDC2 是 m 6 在精子发生期间起关键作用的 A 绑定蛋白质。 | Phillip J Hsu Yunfei Zhu Honghui Ma Yueshuai Guo Xiaodan Shi Yuanyuan Liu Meijie Qi Zhike Lu Hailing Shi Jianying Wang Yiwei Cheng Guanzheng Luo Qing Dai Mingxi Liu Xuejiang Guo Jiahao Sha Bin Shen Chuan He | 2017 | Cell Research2017，27，9： | 96 |
| 2 | Flavonoid profiling of a traditional Chinese medicine formula of Huangqin Tang using high performance liquid chromatography显示文摘The quality control processes for herbal medicines have been problematic. Flavonoids are the major active components of Huangqin Tang(HQT, a traditional Chinese medicine formula). In this study, we used a combinative method approach consisting of chromatographic fingerprinting(high performance liquid chromatography; HPLC), quantitative methods and a pharmacodynamic evaluation model to analyze the flavonoids of HQT obtained from different sources. Ten batches of HQT were analyzed by the HPLC fingerprinting method and 26 common peaks were detected, of which 23 peaks corresponded with the chemical profile of HQT. In addition, 11 major compounds were identified by LC–MS analysis(liquid chromatography–tandem mass spectrometer; LC–MS^n) and quantified by the HPLC quantitative method approach. The studied10 batches of HQT were found to be homogeneous in their composition with a similarity between 0.990 and1.000. The distribution of the 11 identified compounds was found to be very similar among the batches. Only slight pharmacodynamic differences were detected between the different batches, confirming the homogeneity of HQT. The results of this study prove that the combination of chromatographic fingerprinting and quantitative analysis can be readily used for comprehensive quality control of herbal medicines. | Tao Li Shuaixing Zhuang Yiwei Wang Yanli Wang Weihao Wang Huihui Zhang Li Chen Dunfang Wang Zhongming Zhou Weipeng Yang | 2016 | Acta Pharmaceutica Sinica B2016，6，2： | 19 |
| 3 | From concept to reality-A review to the primary test stand and its preliminary application in high energy density physics显示文摘Pulsed power technology,whereas the electrical energy stored in a relative long period is released in much shorter timescale,is an efficient method to create high energy density physics(HEDP)conditions in laboratory.Around the beginning of this century,China Academy of Engineering Physics(CAEP)began to build some experimental facilities for HEDP investigations,among which the Primary Test Stand(PTS),a multi-module pulsed power facility with a nominal current of 10 MA and a current rising time~90 ns,is an important achievement on the roadmap of the electro-magnetically driven inertial confinement fusion(ICF)researches.PTS is the first pulsed power facility beyond 10 TW in China.Therefore,all the technologies have to be demonstrated,and all the engineering issues have to be overcome.In this article,the research outline,key technologies and the preliminary HEDP experiments are reviewed.Prospects on HEDP research on PTS and pulsed power development for the next step are also discussed. | Jianjun Deng Weiping Xie Shuping Feng Meng Wang Hongtao Li Shengyi Song Minghe Xia Ji Ce An He Qing Tian Yuanchao Gu Yongchao Guan Bin Wei Xianbin Huang Xiaodong Ren Jiakun Dan Jing Li Shaotong Zhou Hongchun Cai Siqun Zhang Kunlun Wang Qiang Xu Yujuan Wang Zhaohui Zhang Guilin Wang Shuai Guo Yi He Yiwei Zhou Zhanji Zhang Libing Yang Wenkang Zou | 2016 | Matter and Radiation at Extremes2016，1，1： | 19 |
| 4 | Adverse factors increase preeclampsia-like changes in pregnant mice with abnormal lipid metabolism显示文摘 | Ding Xiaoyan Yang Zi Han Yiwei Yu Huan | 2014 | Chinese Medical Journal2014，，15： | 18 |
| 5 | Small molecule inhibitors reveal allosteric regulation of USP14 via steric blockade显示文摘The ubiquitin system is important for drug discovery,and the discovery of selective small-molecule inhibitors of deubiquitinating enzymes (DUBs) remains an active yet extremely challenging task. With a few exceptions,previously developed inhibitors have been found to bind the evolutionarily conserved catalytic centers of DUBs,resulting in poor selectivity. The small molecule IU1 was the first-ever specific inhibitor identified and exhibited surprisingly excellent selectivity for USP14 over other DUBs. However,the molecular mechanism for this selectivity was elusive. Herein,we report the high-resolution co-crystal structures of the catalytic domain of USP14 bound to IU1 and three IU1 derivatives. All the structures of these complexes indicate that IU1 and its analogs bind to a previously unknown steric binding site in USP14,thus blocking the access of the C-terminus of ubiquitin to the active site of USP14 and abrogating USP14 activity. Importantly,this steric site in USP14 is very unique,as suggested by structural alignments of USP14 with several known DUB X-ray structures. These results,in conjunction with biochemical characterization,Indicate a coherent steric blockade mechanism for U5P14 inhibition by compounds of the IU series. In light of the recent report of steric blockade of USP7 by FT671,this work suggests a potential generally applicable allosteric mechanism for the regulation of DUBs via steric blockade,as showcased by our discovery of IU1-248 which is 10-fold more potent than IU1. | Yiwei Wang Yuxuan Jiang Shan Ding Jiawang Li Ningjing Song Yujing Ren Danning Hong Cai Wu Bin Li Feng Wang Wei He Jiawei Wang Ziqing Mei | 2018 | Cell Research2018，28，12： | 15 |
| 6 | Generation of a precise Oct4-hrGFP knockin cynomolgus monkey model via CRISPR/Cas9-assisted homologous recombination显示文摘 | Yiqiang Cui Yuyu Niu Jiankui Zhou Yongchang Chen Yiwei Cheng Shangang Li Zongyong Ai Chu Chu Hong Wang Bo Zheng Xuejin Chen Jiahao Sha Xuejiang Guo Xingxu Huang Weizhi Ji | 2018 | Cell Research2018，28，3： | 13 |
| 7 | Design Schemes and Comparison Research of the End-effector of Large Space Manipulator显示文摘The end-effector of the large space manipulator is employed to assist the manipulator in handling and manipulating large payloads on orbit.Currently,there are few researches about the end-effector,and the existing end-effectors have some disadvantages,such as poor misalignment tolerance capability and complex mechanical components.According to the end positioning errors and the residual vibration characters of the large space manipulators,two basic performance requirements of the end-effector which include the capabilities of misalignment tolerance and soft capture are proposed.And the end-effector should accommodate the following misalignments of the mechanical interface.The translation misalignments in axial and radial directions and the angular misalignments in roll,pitch and yaw are ±100 mm,100 mm,±10°,±15°,±15°,respectively.Seven end-effector schemes are presented and the capabilities of misalignment tolerance and soft capture are analyzed elementarily.The three fingers-three petals end-effector and the steel cable-snared end-effector are the most feasible schemes among the seven schemes,and they are designed in detail.The capabilities of misalignment tolerance and soft capture are validated and evaluated,through the experiment on the micro-gravity simulating device and the dynamic analysis in ADAMS software.The results show that the misalignment tolerance capabilities of these two schemes could satisfy the requirement.And the translation misalignment tolerances in axial and radial directions and the angular misalignment tolerances in roll,pitch and yaw of the steel cable-snared end-effector are 30mm,15mm,6°,3° and 3° larger than those of the three fingers-three petals end-effector,respectively.And the contact force of the steel cable-snared end-effector is smaller and smoother than that of the three fingers-three petals end-effector.The end-effector schemes and research methods are beneficial to the developments of the large space manipulator end-effctor and the space docking mechanism. | FENG Fei LIU Yiwei LIU Hong CAI Hegao | 2012 | Chinese Journal of Mechanical Engineering2012，25，4： | 13 |
| 8 | Synergistic effect between Sn and K promoters on supported platinum catalyst for isobutane dehydrogenation显示文摘Catalytic dehydrogenation of isobutane has recently received considerable attention because of the increasing demand for isobutene.In this study,the synergistic effect between Sn and K on PtSnK/γ-Al2O3 catalysts has been investigated by changing the content of Sn.It was found that with the presence of potassium,suitable addition of Sn could not only increase the metal dispersion,but also reduce the catalyst acidity.In these cases,the synergistic effect could also strengthen the interactions between the metal and support,which resulted in an increase in both catalytic activity and stability.In our experiments,Pt-0.6SnK/Al catalyst exhibited the lowest deactivation rate (12.4%) and showed a selectivity to isobutene higher than 94% at the isobutane conversion of about 45.3% after running the reaction for 6 h.However,with the excessive loading of Sn,surface property of active sites and the interactions between metal and support were changed.As a result,the initial optimal ratio between the metallic function and acid function would be destroyed,which was disadvantageous to the reaction. | Yiwei Zhang Yuming Zhou Lihui Wan Mengwei Xue Yongzheng Duan Xuan Liu | 2011 | Journal of Natural Gas Chemistry2011，20，6： | 12 |
| 9 | Synthesis and Enhanced Photocatalytic Activity of Regularly Shaped Cu2O Nanowire Polyhedra显示文摘一系列唯一的 nanowire 上层建筑, Cu2O nanowire polyhedra,通过一条划算的热水的线路被综合了。nanowire polyhedra 的三种类型,也就是 octahedra,凹面 octahedra,和昆虫,被面对羧基的酸与 o-anisidine 或 o-phenetidine 减少含铜的醋酸盐在高词法的收益(90%) 形成。这些 Cu2O nanowire polyhedra 的体系结构被电子显微镜学检验,它在多面的轮廓以内揭示了订的、高度排列的 Cu2O nanowires。Cu2O nanowire polyhedra 的生长被取向和被羧基的酸的增加调整的 nanowire 分支的生长率控制。比作样品在最近的文学报导了的 Cu2O, Cu2O nanowire octahedra 展览显著地在可见的光下面面对 H2O2 为染料降级提高了 photocatalytic 活动,可能,由于高密度的费用搬运人,从有他们的专辑的分叉的 nanowires 的 photoexcited 组织。另外,在当为染料的直接 photodegradation 的 H2O2 不在时的 Cu2O 的 photocatalytic 活动的最近的文学的讨论似乎可疑。 | Jing Shi Jin LI Xiaojian Huang Yiwei Tan | 2011 | Nano Research2011，4，5： | 12 |
| 10 | Dihydroartemisinin regulates the immune system by promotion of CD8^+T lymphocytes and suppression of B cell responses显示文摘Artemisia annua is an anti-fever herbal medicine first described in traditional Chinese medicine 1,000 years ago.Artemisinin,the extract of A.annua,and its derivatives(dihydroartemisinin(DHA),artemether,and artesunate)have been used for the treatment of malaria with substantial efficacy.Recently,DHA has also been tested for the treatment of lupus erythematosus,indicating that it may function to balance the immune response in immunocompromised individuals.In the present study,the regulatory effect of artemisinin on the murine immune system was systematically investigated in mice infected with two different protozoan parasites(Toxoplasma gondii and Plasmodium berghei).Our results revealed that the mouse spleen index significantly increased(spleen enlargement)in the healthy mice after DHA administration primarily due to the generation of an extra number of lymphocytes and CD8^+T lymphocytes in both the spleen and circulation.DHA could increase the proportion of T helper cells and CD8^+T cells,as well as decrease the number of splenic and circulatory B cells.Further,DHA could reduce the production of proinflammatory cytokines.Our study revealed that apart from their anti-parasitic activity,artemisinin and its derivatives can also actively modulate the immune system to directly benefit the host. | Ting Zhang Yiwei Zhang Ning Jiang Xu Zhao Xiaoyu Sang Na Yang Ying Feng Ran Chen Qijun Chen | 2020 | Science China(Life Sciences)2020，63，5： | 12 |
| 11 | Trichosanthin enhances anti-tumor immune response in a murine Lewis lung cancer model by boosting the interaction between TSLC1 and CRTAM显示文摘Trichosanthin(TCS),extracted from the Chinese medicinal herb Trichosanthes kirilowi,has shown promise for the inhibition of tumor growth.However,its immunomodulatory effect on tumor–host interaction remains unknown.In this study,we focused on the effect of TCS on murine anti-tumor immune response in the 3LL Lewis lung carcinoma tumor model and explored the possible molecular pathways involved.In addition to inhibiting cell proliferation and inducing apoptosis in the 3LL tumor,TCS retarded tumor growth and prolonged mouse survival more significantly in C57BL/6 immunocompetent mice than in nude mice.This reflected the fact that the host immune system was involved in tumor eradication.Using FACS analysis,we found that TCS increased the percentage of effector T cells,particularly Interferon-gamma(IFN-c)producing CD41 and CD81 T cells from tumor-bearing mice.TCS also promoted the vigorous proliferation of antigen-specific effector T cells,markedly increased Th1 cytokine secretion and elicited more memory T cells in tumor-bearing mice,consequently enhancing the anti-tumor response and inducing immune protection.Furthermore,we found that TCS upregulated the expression of tumor suppressor in lung cancer 1(TSLC1)in 3LL tumor cells and the expression of its ligand,class I-restricted T cell-associated molecule(CRTAM),in effector T cells.Blocking TSLC1 expression with small interfering RNA(siRNA)significantly eliminated the effects of TCS on the proliferation and cytokine secretion of effector T cells,suggesting that TCS enhances anti-tumor immune response at least partially by boosting the interaction between TSLC1 and CRTAM.Collectively,our data demonstrate that TCS not only affects tumor cells directly,but also enhances anti-tumor immunity via the interaction between TSLC1 and CRTAM.These findings may lead to the development of a novel approach for tumor regression. | Yuchan Cai Shudao Xiong Yijie Zheng Feifei Luo Pei Jiang Yiwei Chu | 2011 | Cellular & Molecular Immunology2011，8，4： | 12 |
| 12 | Degree of coupling and coordination of eco-economic system and the influencing factors: a case study in Yanchi County, Ningxia Hui Autonomous Region, China显示文摘Based on the statistical data, we analyzed and evaluated the degree of coupling and coordination of the eco-economic system in Yanchi County for the period spanning from 1983 to 2014. The eco-economic system can be divided into socioeconomic and ecological sub-systems and their relationship can reveal the interaction state between the two sub-systems and help the local government to establish a coordinated development mode. An index system was constructed to assess the development of the two sub-systems before the evaluation of the degree of coupling and coordination. The principal component regression analysis was adopted to quantitatively assess the influences of natural, economic and social factors on the degree of coupling and coordination of the eco-economic system. Results showed that, from 1983 to 2014, the development trends of both sub-systems were increasing with the ecological sub-system having more fluctuations. The degree of coupling and coordination of the eco-economic system in the study area increased gradually from 1983 to 2014, but experienced five different development stages from the verge of disorder to favorable coordination. The development of the local social and economic conditions was the most important factor influencing the degree of coupling and coordination. The second most important factor was the financial support from the local government. In addition, the environment protection policies also played undeniable roles. Due to the diversity of the influence factors, the government should take comprehensive measures to promote the sustainable development of the eco-economic system. | LU Huiling ZHOU Lihua CHEN Yong AN Yiwei HOU Caixia | 2017 | Journal of Arid Land2017，9，3： | 12 |
| 13 | Effect of Strontium Addition to Platinum Catalyst for Propane Dehydrogenation显示文摘PtSnSr/HZSM-5 catalysts with different amounts of strontium were prepared by sequential impregnation method,and characterized by BET analysis,TEM,NH3-TPD,H2-TPR,TPO and TG techniques.The results showed that the addition of strontium could modify the characteristics and properties of both acid function and metal function of Pt-Sn-based catalysts.In this case,PtSnSr/HZSM-5 catalyst with an appropriate amount of Sr(1.2%) showed higher catalytic activity and lower amount of coke deposits than PtSn/HZSM-5 catalyst.However,excessive loading of Sr could facilitate the reduction of Sn,which was unfavorable to the reaction.Afterwards,1.0 m% of Na was added into the PtSnSr(1.2%)/HZSM-5 catalyst to improve the catalytic performance in propane dehydrogenation,and this catalyst displayed the best catalytic performance during our experiments.After having been subjected to reaction for 5h,the PtSnNa(1.0%)Sr(1.2%)/HZSM-5 catalyst had achieved a higher than 95% selectivity towards propene along with a corresponding propane conversion rate of 32.2%. | Huang Li Zhou Shijian Zhou Yuming Zhang Yiwei Zhang Shaobo Xu Jun | 2012 | China Petroleum Processing & Petrochemical Technology2012，14，3： | 11 |
| 14 | Enhancement of antitumor immunity by low-dose total body irradiation is associated with selectively decreasing the proportion and number of T regulatory cells显示文摘低剂量的全部的身体照耀(LTBI ) 主要为杀死的有免疫力的改进而非房间在 somecancers 的治疗被使用。然而,位于 LTBI 下面的 themechanism 仍然保持未知。在这研究,由分析淋巴细胞的 theimmune 模式,我们发现 CD4+CD25+Foxp3+regulatory T 房间的百分比和 absolutenumber 显著地在跟随 treatmentwith LTBI 的天真的老鼠被减少。相反, CD4+CD44+/CD8+CD44+effector-memory T 房间极大地被增加。重要地,天真的老鼠 treatedwith 在 LTBI 下面的树枝状的 cell-gp100 肿瘤疫苗导致了 enhancementof 抗原特定的增长和 cytotoxicity 以及干扰素 -- 对 F10 黑瘤肿瘤挑战的纬(IFN- 纬) 分泌物,与 treatmentwith 相比肿瘤疫苗或 LTBI 独自一个。因而,处理 resultedin 减少的肿瘤负担和延长老鼠幸存。我们 antitumor 免疫的数据 demonstratethat LTBI 鈥檚 改进主要是有选择地减少的 associatedwith T 规章的房间的比例和数字,在 antitumor 暗示 LTBI 和 tumorvaccine 的联合的潜在的应用程序治疗。 | Shudao Xiong Lei Zhang Yiwei Chu | 2010 | Cellular & Molecular Immunology2010，7，2： | 11 |
| 15 | Reversing design methodology of investment casting die profile based on ProCAST显示文摘Turbine blade is one of the critical components of aircraft engine.The performance of the engine depends on the shape and dimensions of components,but superalloy blade material cannot be easily machined.Although investment casting is an ideal process for such net-shape components,it requires an accurate determination of the casting-die profile.In this paper,a reversing design methodology for investment casting die using ProCAST is proposed.By combining the methods of simplifying grid files and quick sorting,the efficiency of sorting and matching can be largely improved.Further,the mould/die cavity anti-deformation system can be easily built.With ProCAST,the optimized die profile for investment casting can be established. | Dou Yangqing Bu Kun Dou Yangliu Dong Yiwei | 2010 | China Foundry2010，7，2： | 10 |
| 16 | Relevant experience learning:A deep reinforcement learning method for UAV autonomous motion planning in complex unknown environments显示文摘Unmanned Aerial Vehicles(UAVs)play a vital role in military warfare.In a variety of battlefield mission scenarios,UAVs are required to safely fly to designated locations without human intervention.Therefore,finding a suitable method to solve the UAV Autonomous Motion Planning(AMP)problem can improve the success rate of UAV missions to a certain extent.In recent years,many studies have used Deep Reinforcement Learning(DRL)methods to address the AMP problem and have achieved good results.From the perspective of sampling,this paper designs a sampling method with double-screening,combines it with the Deep Deterministic Policy Gradient(DDPG)algorithm,and proposes the Relevant Experience Learning-DDPG(REL-DDPG)algorithm.The REL-DDPG algorithm uses a Prioritized Experience Replay(PER)mechanism to break the correlation of continuous experiences in the experience pool,finds the experiences most similar to the current state to learn according to the theory in human education,and expands the influence of the learning process on action selection at the current state.All experiments are applied in a complex unknown simulation environment constructed based on the parameters of a real UAV.The training experiments show that REL-DDPG improves the convergence speed and the convergence result compared to the state-of-the-art DDPG algorithm,while the testing experiments show the applicability of the algorithm and investigate the performance under different parameter conditions. | Zijian HU Xiaoguang GAO Kaifang WAN Yiwei ZHAI Qianglong WANG | 2021 | Chinese Journal of Aeronautics2021，34，12： | 10 |
| 17 | Deformation behavior of non-rigid airships in wind tunnel tests显示文摘Deformation behavior of non-rigid airships in wind tunnel tests is studied by considering three factors, including internal pressure, flow velocity and angle of attack. Fiber Bragg grating strain sensors are used to measure the deformation of non-rigid airships. Wind tunnel tests in the case of different flow velocities and angles of attack are conducted. The measurement results reveal that the airship deformation is in proportion to internal pressure. For the tensile region,the airship deformation is in proportion to flow velocity. Effects of angle of attack on structural deformation are more complicated and there is no clear relationship existing between airship deformation and angle of attack. | Lingling LU Hongwei SONG Yiwei WANG Chenguang HUANG | 2019 | Chinese Journal of Aeronautics2019，32，3： | 9 |
| 18 | In vitro inhibitory and pro-apoptotic effect of Stellera Chamaejasme LExtract on human lung cancer cell line NCI-H157显示文摘OBJECTIVE:To investigate the inhibitory and pro-apoptotic effect of Stellera Chamaejasme L extract(ESC) in vitro.METHODS:ESC was first extracted with ethanol,and then washed using a polyamide column with 60% ethanol.ESC was then decompressively recycled and vacuum dried at room temperature to obtain active fractions.Subsequently,the cytotoxic and apoptotic effects of ESC on NCI-H157 human lung cancer cells were determined.RESULTS:The results showed that ESC was rich in isomers of Chamaejasminor,neochamaejasmine and Sikokianin.ESC had significant cytotoxicity against NCI-H157 cells,with an IC 50 of approximately 18.50 μg.mL-1.ESC caused significant increase in total apoptotic rate,the activity of caspase 3 and 8,and Fas protein expression(P<0.05).CONCLUSION:The inhibitory effect of ESC on NCI-H157 tumor cells might partly be attributed to its apoptotic induction through activation of the Fas death receptor pathway. | Xiaoni Liu Yujie Li Qing Yang Ying Chen Xiaogang Weng Yiwei Wang Ning Li Xiaoxin Zhu | 2012 | Journal of Traditional Chinese Medicine2012，32，3： | 8 |
| 19 | Effects of RapidEye Imagery's Red-edge Band and Vegetation Indices on Land Cover Classification in an Arid Region显示文摘Land cover classification(LCC) in arid regions is of great significance to the assessment, prediction, and management of land desertification. Some studies have shown that the red-edge band of RapidE ye images was effective for vegetation identification and could improve LCC accuracy. However, there has been no investigation of the effects of RapidE ye images' red-edge band and vegetation indices on LCC in arid regions where there are spectrally similar land covers mixed with very high or low vegetation coverage information and bare land. This study focused on a typical inland arid desert region located in Dunhuang Basin of northwestern China. First, five feature sets including or excluding the red-edge band and vegetation indices were constructed. Then, a land cover classification system involving plant communities was developed. Finally, random forest algorithm-based models with different feature sets were utilized for LCC. The conclusions drawn were as follows: 1) the red-edge band showed slight contribution to LCC accuracy; 2) vegetation indices had a significant positive effect on LCC; 3) simultaneous addition of the red-edge band and vegetation indices achieved a significant overall accuracy improvement(3.46% from 86.67%). In general, vegetation indices had larger effect than the red-edge band, and simultaneous addition of them significantly increased the accuracy of LCC in arid regions. | LI Xianju CHEN Gang LIU Jingyi CHEN Weitao CHENG Xinwen LIAO Yiwei | 2017 | Chinese Geographical Science2017，27，5： | 8 |
| 20 | Abnormal expression of bHLH3 disrupts a flavonoid homeostasis network,causing differences in pigment composition among mulberry fruits显示文摘Mulberry fruits with high concentrations of anthocyanins are favored by consumers because of their good taste,bright color,and high nutritional value.However,neither the regulatory mechanism controlling flavonoid biosynthesis in mulberry nor the molecular basis of different mulberry fruit colors is fully understood.Here,we report that a flavonoid homeostasis network comprising activation and feedback regulation mechanisms determines mulberry fruit color.In vitro and in vivo assays showed that MYBA-bHLH3-TTG1 regulates the biosynthesis of anthocyanins,while TT2L1 and TT2L2 work with bHLH3 or GL3 and form a MYB-bHLH-WD40(MBW)complex with TTG1 to regulate proanthocyanidin(PA)synthesis.Functional and expression analyses showed that bHLH3 is a key regulator of the regulatory network controlling mulberry fruit coloration and that MYB4 is activated by MBW complexes and participates in negative feedback control of the regulatory network to balance the accumulation of anthocyanins and proanthocyanidins.Our research demonstrates that the interaction between bHLH3 and MYB4 in the homeostasis regulatory network ensures that the fruits accumulate desirable flavonoids and that this network is stable in pigmentrich mulberry fruits.However,the abnormal expression of bHLH3 disrupts the balance of the network and redirects flavonoid metabolic flux in pale-colored fruits,resulting in differences in the levels and proportions of anthocyanins,flavones,and flavonols among differently colored mulberry fruits(red,yellow,and white).The results of our study reveal the molecular basis of the diversity of mulberry fruit colors. | Han Li Zhen Yang Qiwei Zeng Shibo Wang Yiwei Luo Yan Huang Youchao Xin Ningjia He | 2020 | Horticulture Research2020，7，1： | 8 |
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