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| 1 | ζ-Carotene Isomerase Suppresses Tillering in Rice through the Coordinated Biosynthesis of Strigolactone and Abscisic Acid显示文摘Rice tillering is an important agronomic trait affecting grain yield.Here,we identified a high-tillering mutant tillering20(t20),which could be restored to the wild type by treatment with the strigolactone(SL)analog rac-GR24.T20 encodes a chloroplast ζ-carotene isomerase(Z-ISO),which is involved in the biosynthesis of ca-rotenoids and their metabolites,SL and abscisic acid(ABA).The t20 mutant has reduced SL and ABA,raising the question of how SL and ABA biosynthesis is coordinated,and whether they have overlapping functions in tillering.We discovered that rac-GR24 stimulated T20 expression and enhanced all-trans-p-carotene biosynthesis.Importantly,rac-GR24 also stimulated expression of Oryza sativa 9-CIS-EPOXY-CAROTENOID DIOXYGENASE 1(OsNCED1)through induction of Oryza sativa HOMEOBOX12(0sHOX12),promoting ABA biosynthesis in shoot base.On the other hand,ABA treatment significantly repressed SL biosynthesis and the ABA biosynthetic mutants displayed elevated SL biosynthesis.ABA treatment reduced the number of basal tillers in both t20 and wild-type plants.Furthermore,while ABA-deficient mu-tants aba1 and aba2 had the same number of basal tillers as wild type,they had more unproductive upper tillers at maturity.This work demonstrates complex interactions in the biosynthesis of carotenoid,SLs and ABA,and reveals a role for ABA in the regulation of rice tillering. | Xue Liu Qingliang Hu Jijun Yan Kai Sun Yan Liang Meiru Jia Xiangbing Meng Shuang Fang Yiqin Wang Yanhui Jing Guifu Liu Dianxing Wu Chengcai Chu Steven M.Smith Jinfang Chu Yonghong Wang Jiayang Li Bing Wang | 2020 | Molecular Plant2020，13，12： | 11 |
| 2 | Review of the biological and engineering aspects of algae to fuels approach显示文摘Current biofuel production relies on limited arable lands on the earth,and is impossible to meet the biofuel demands.Oil producing algae are alternative biofuel feedstock with potential to meet the world’s ambitious goal to replace fossil fuels.This review provides an overview of the biological and engineering aspects in the production and processing technologies and recent advances in research and development in the algae to fuels approach.The article covers biology,selection and genetic modification of algae species and strains,production systems design,culture media and light management,harvest and dewatering,downstream processing,and environment and economic assessment.Despite the many advances made over several decades,commercialization of algal fuels remains challenging chiefly because of the techno-economic constraints.Technological breakthroughs in all major aspects must take place before commercial production of algal fuels becomes economically viable. | Paul Chen Min Min Yifeng Chen Liang Wang Yecong Li Qin Chen Chenguang Wang Yiqin Wan Xiaoquan Wang Yanling Cheng Shaobo Deng Kevin Hennessy Xiangyang Lin Yuhuan Liu Yingkuan Wang Blanca Martinez Roger Ruan | 2009 | International Journal of Agricultural and Biological Engineering2009，2，4： | 9 |
| 3 | Stepwise-Nanocavity-Assisted Transmissive Color Filter Array Microprints显示文摘Visible-light color flters using patterned nanostructures have attracted much interest due to their various advantages such as compactness,enhanced stability,and environmental friendliness compared with traditional pigment or dye-based optical flters.While most existing studies are based on planar nanostructures with lateral variation in size,shape,and arrangement,the vertical dimension of structures is a long-ignored degree of freedom for the structural colors.Herein,we demonstrate a synthetic platform for transmissive color flter array by coordinated manipulations between height-varying nanocavities and their lateral flling fractions.Te thickness variation of those nanocavities has been fully deployed as an alternative degree of freedom,yielding vivid colors with wide gamut and excellent saturation.Experimental results show that the color-rendering capability of the pixelated nanocavities can be still retained as pixels are miniaturized to 500 nm.Crosstalk between closely spaced pixels of a Bayer color flter arrangement was calculated,showing minimal crosstalk for 1μm2 square subpixels.Our work provides an approach to designing and fabricating ultracompact color flter arrays for various potential applications including stained-glass microprints,microspectrometers,and high-resolution image sensing systems. | Yasi Wang Mengjie Zheng Qifeng Ruan Yanming Zhou Yiqin Chen Peng Dai Zhengmei Yang Zihao Lin Yuxiang Long Ying Li Na Liu Cheng-Wei Qiu Joel K.W.Yang Huigao Duan | 2018 | Research2018，，1： | 8 |
| 4 | Symptom selection for multi-label data of inquiry diagnosis in traditional Chinese medicine显示文摘In traditional Chinese medicine(TCM) diagnosis,a patient may be associated with more than one syndrome tags,and its computer-aided diagnosis is a typical application in the domain of multi-label learning of high-dimensional data.It is common that a great deal of symptoms can occur in traditional Chinese medical diagnosis,which affects the modeling of diagnostic algorithm.Feature selection entails choosing the smallest feature subset of relevant symptoms,and maximizing the generalization performance of the model.At present there are rare researches on feature selection on multi-label data.A hybrid optimization technique is introduced to symptom selection for multi-label data in TCM diagnosis in this paper,and modeling is made by means of four multi-label learning algorithms like k nearest neighbors,etc.We compare the performance of the algorithm with the current popular dimension reduction algorithms like MEFS(embedded feature selection for multi-Label learning),MDDM(multi-label dimensionality reduction via dependence maximization) on the UCI Yeast gene functional data set and an inquiry diagnosis dataset of coronary heart disease(CHD).Experimental results show that the algorithm we present has significantly improved the performance.In particular,the improvement on the average precision for the classifier is up to 10.62% and 14.54%.Syndrome inquiry modeling of CHD in TCM is realized in this paper,providing effective reference for the diagnosis of CHD and analysis of other multi-label data. | SHAO Huan LI GuoZheng LIU GuoPing WANG YiQin | 2013 | Science China(Information Sciences)2013，56，5： | 8 |
| 5 | Big Grain3, encoding a purine permease, regulates grain size via modulating cytokinin transport in rice显示文摘Grain size is an important agronomic trait affecting grain yield,but the underlying molecular mechanisms remain to be elucidated.Here,we isolated a dominant mutant,big grainy(bg3-D),which exhibits a remarkable increase of grain size caused by activation of the PURINE PERMEASE gene,OsPUP4.BC3/OSPUP4 is predominantly expressed in vascular tissues and is specifically suppressed by exogenous cytokinin application.Hormone profiling revealed that the distribution of different cytokinin forms,in roots and shoots of the bg3-D mutant,is altered.Quantitative reverse transcription-PCR(qRT-PCR)analysis indicated that expression of rice cytokinin type-A RESPONSE REGULATOR(OsRR)genes is enhanced in the roots of the bgj-D mutant.These results suggest that OSPUP4 might contribute to the long-distance transport of cytokinin,by reinforcing cytokinin loading into vascular bundle cells.Furthermore,plants overexpressing OsPUP7,the closest homolog of OsPUP4,also exhibited a similar phenotype to the bg3-D mutant.Interestingly,subcellular localization demonstrated that OSPUP4 was localized on the plasma membrane,whereas OSPUP7 was localized to the endoplasmic reticulum.Based on these findings,we propose that OSPUP4 and OSPUP7 function in a linear pathway to direct cytokinin cell-to-cell transport,affecting both its long-distance movement and local allocation. | Yunhua Xiao Dapu Liu Guoxia Zhang Shaopei Gao Linchuan Liu Fan Xu Ronghui Che Yiqin Wang Hongning Tong Chengcai Chu | 2019 | Journal of Integrative Plant Biology2019，61，5： | 7 |
| 6 | 3D-Integrated metasurfaces for full-colour holography显示文摘Metasurfaces enable the design of optical elements by engineering the wavefront of light at the subwavelength scale.Due to their ultrathin and compact characteristics,metasurfaces possess great potential to integrate multiple functions in optoelectronic systems for optical device miniaturisation.However,current research based on multiplexing in the 2D plane has not fully utilised the capabilities of metasurfaces for multi-tasking applications.Here,we demonstrate a 3D-integrated metasurface device by stacking a hologram metasurface on a monolithic Fabry–Pérot cavity-based colour filter microarray to simultaneously achieve low-crosstalk,polarisation-independent,high-efficiency,full-colour holography,and microprint.The dual functions of the device outline a novel scheme for data recording,security encryption,colour displays,and information processing.Our 3D integration concept can be extended to achieve multi-tasking flat optical systems by including a variety of functional metasurface layers,such as polarizers,metalenses,and others. | Yueqiang Hu Xuhao Luo Yiqin Chen Qing Liu Xin Li Yasi Wang Na Liu Huigao Duan | 2019 | Light(Science & Applications)2019，8，1： | 6 |
| 7 | Expression patterns of the rice class I metallothionein gene family in response to lead stress in rice seedlings and functional complementation of its members in lead-sensitive yeast cells显示文摘Metallothioneins (MTs) are a group of low molecular mass and cysteine-rich proteins that can chelate heavy-metal ions. In this paper,Northern blot analysis was used to investigate the influence of lead stress on the expression patterns of 10 rice class I MT genes (OsMT-Is) in rice seedlings. With the ex-ception of OsMT-I-3b,the data demonstrate dynamic changes of 9 OsMT-I transcripts in response to Pb2+ treatment in rice seedling roots. Of these genes,transcription of OsMT-I-1a,OsMT-I-1b,OsMT-I-2c,OsMT-I-4a,OsMT-I-4b and OsMT-I-4c increased significantly,while transcription of OsMT-I-2a and OsMT-I-3a increased marginally. In contrast,the expression of OsMT-I-2b was inhibited. Pb2+ induced the expression of 6 OsMT-I genes in seedling shoots,but had no obvious effects on the expression of OsMT-I-1a,OsMT-I-1b,OsMT-I-4a and OsMT-I-4b. All the 10 OsMT-Is had enhanced lead tolerance when heterologously expressed in lead-sensitive yeast mutant cells. These results provide an expression profile of the rice MT gene family in response to Pb2+ stress in rice seedlings and demonstrate in-creased lead tolerance in sensitive yeast mutant cells expressing OsMT-Is. This study lays a foundation for further analysis of the role of the rice MT gene family in respond to Pb2+ stress. | XU YuFeng ZHOU GongKe ZHOU Lu LI YiQin LIU JinYuan | 2007 | Chinese Science Bulletin2007，52，16： | 5 |
| 8 | Catalytic microwave assisted pyrolysis of aspen显示文摘The effect of catalysts on the microwave pyrolysis of aspen pellets was studied.A range of chlorides,nitrates and metal-oxides were added at 2%of the aspen mass(air dry aspen pellet weight basis).Chlorides in particular were found to favor liquid yield,especially the yield of water phase residue.Average liquid yield with added chlorides was 41%mass of the total biomass input,compared to 35%mass without catalyst.Metal-oxides were found to favor pyrolysis heavy oil,and thus total oil yield,since the yield of light oils seemed to be fairly constant.Nitrates were found to favor pyrolysis gas production.Pure light oils and light oils blended with diesel were found to be a potential diesel fuel substitute. | Johannes Moen Changyan Yang Bo Zhang Hanwu Lei Kevin Hennessy Yiqin Wan Zhiping Le Yuhuan Liu Paul Chen Roger Ruan | 2009 | International Journal of Agricultural and Biological Engineering2009，2，4： | 5 |
| 9 | Microwave-Assisted Decarboxylation of Sodium Oleate and Renewable Hydrocarbon Fuel Production显示文摘The carboxyl terminal of sodium oleate has a stronger polarity than that of oleic acid;this terminal is more likely to be dipole polarized and ionically conductive in a microwave field.Sodium oleate was used as the model compound to study the decarboxylation of oleic acid leading to hydrocarbon formation via microwave-assisted pyrolysis technology.The pyrolysis gas,liquid,and solid products were precisely analyzed to deduce the mechanism for decarboxylation of sodium oleate.Microwave energy was able to selectively heat the carboxyl terminal of sodium oleate.During decarboxylation,the double bond in the long hydrocarbon chain formed a p-πconjugated system with the carbanion intermediate.The resulting p-πconjugated system was more stable and beneficial to the pyrolysis reaction(decarboxylation,terminal allylation,isomerization,and aromatization).The physical properties of pyrolysis liquid were generally similar to those of diesel fuel,thereby demonstrating the possible use of microwaves for controlling the decarboxylation of sodium oleate in order to manufacture renewable hydrocarbon fuels. | Wang Yunpu Liu Yuhuan Ruan Rongsheng Wen Pingwei Wan Yiqin Zhang Jinsheng | 2013 | China Petroleum Processing & Petrochemical Technology2013，15，3： | 5 |
| 10 | Cloning,expression and characterization of serine palmitoyltransferase(SPT)-like gene subunit(LCB2) from marine Emiliania huxleyi virus(Coccolithovirus)显示文摘The authors have isolated and characterized a novel serine palmitoyltransferase(SPT)-like gene in marine Emiliania huxleyi virus(EhV-99B1).The open-reading frame(ORF) of EhV99B1-SPT encoded a protein of 496 amino acids with a calculated molecular mass of 96 kDa and Ip 6.01.The results of sequence analysis showed that there was about 31%-45% identity in amino acid sequence with other organisms.The maximum likelihood phylogenetic tree suggested that the EhV99B1-SPT gene possibly horizontally transferred from the eukaryote.Hydrophobic profiles of deduced amino acid sequences suggested a hydrophobic,globular and membrane-associated protein with five transmembrane domains(TMDs) motifs.Several potential N-linked glycosylation sites were presented in SPT.These results suggested that EhV99B1-SPT was an integral endoplasmic reticulum membrane protein.Despite lower sequence identity,the secondary and three-dimensional structures predicted showed that the 'pocket' structure element composed of 2α-helices and 4βsheets was the catalytic center of this enzyme,with a typical conserved 'TFTKSFG' active site in the N-terminal region and was very close to those of prokaryotic organisms.However,the N-terminal domain of EhV99B1-SPT most closely resembled the LCB2 catalysis subunit and the C-terminal domain most closely resembled the LCB1 regulatory subunit of other organisms which together formed a spherical molecule.This 'chimera' was highly similar to the prokaryotic homologous SPT.For a functional identification,the EhV99B1-LCB2 subunit gene was expressed in Escherichia coli,which resulted in significant accumulation of new sphingolipid in E.coli cells. | LIU Xuhong ZHENG Tianling CAI Yiqin LIU Jingwen | 2012 | Acta Oceanologica Sinica2012，31，6： | 4 |
| 11 | Fractionation and characterization of bio-oil from microwave-assisted pyrolysis of corn stover显示文摘Bio-oil from thermochemical conversion of biomass is a complex mixture of polar and non-polar compounds,and cannot be used directly as a combustion engine fuel due to its low heating value,high viscosity,chemical instability,and incomplete volatility.Mixed-solvent extraction was developed to fractionate bio-oils from microwave-assisted pyrolysis of corn stover to produce light oil(a mixture of light and inflammable fuel oil components from bio-oil)with low viscosity and low combustion residue,and high value chemicals.Different fractions from bio-oil were characterized using GC/MS and TG,and a major chemical(hydroxy-butanedioic acid diethyl ester)was separated. | Changyan Yang Bo Zhang Johannes Moen Kevin Hennessy Yuhuan Liu Xiangyang Lin Yiqin Wan Hanwu Lei Paul Chen Roger Ruan | 2010 | International Journal of Agricultural and Biological Engineering2010，3，3： | 4 |
| 12 | Structural insights into a dual-specificity histone demethylase ceKDM7A from Caenorhabditis elegans显示文摘Histone 离氨酸 methylation 能被 JmjC 包含域的蛋白质在 sequence-state-specific 和 methylation-state-specific 举止移开。然而,底层特性怎么样,决定,酶怎么被调整,大部分是未知的。我们最近发现了那 ceKDM7A,一个哲学博士 -- 并且 JmjC 包含域的蛋白质,是为 H3K9me2 特定的 histone demethylase, H3K27me2,和 PHD 摸绑定到 H3K4me3 指南在 vivo 的 demethylation 活动。为了为酶的活动和 PHD 的功能提供结构的卓见进分子的机制,摸,我们与包含 H3K4me3, H3K9me2,和 H3K27me2 修正的各种各样的联合的单身者或二肽一起在 apo 形式并且在建筑群解决了酶的六水晶结构。结构显示 H3K9me2 和 H3K27me2 以一种类似的方式与 ceKDM7A 交往,并且肽绑定特性被特定的相互作用的一个网络决定。结构的几何测量也揭示了与 PHD 手指和 H3K9me2 界限联系到 JmjC 领域的那 H3K4me3 从二个分开的分子,建议 trans-histone 肽绑定机制。因此,我们的全身的结构的研究重要地由催化领域而且更多揭示底层识别不仅,为 H3K9me2 和 H3K27me2 的 ceDKM7A 的双特性的分子的机制。 | Ying Yang Lulu Hu Ping Wang Haifeng Hou Yan Lin Yi Liu Ze Li Rui Gong Xiang Feng Lu Zhou Wen Zhang Yuhui Dong Huirong Yang Hanqing Lin Yiqin Wang Charlie Degui Chen Yanhui Xu | 2010 | Cell Research2010，20，8： | 3 |
| 13 | MIT1, encoding a 15-cis-ζ-carotene isomerase, regulates tiller number and stature in rice显示文摘Lateral branching is an important determinant of shoot architecture and crop yield. The plant hormone strigolactone (SL) inhibits lateral bud outgrowth in various plant species. Deficiencies in SL biosynthesis and signal transduction result in excessive outgrowth of lateral buds (Stirnberg et al., 2002;Sorefan et al., 2003). | Lihua Liu Mengmeng Ren Peng Peng Yan Chun Lu Li Jinfeng Zhao Jingjing Fang Lixiang Peng Jijun Yan Jinfang Chu Yiqin Wang Shoujiang Yuan Xueyong Li | 2021 | Journal of Genetics and Genomics2021，48，1： | 3 |
| 14 | Synthesis of Biodiesel Using ZrO_2 Polycrystalline Ceramic Foam Catalyst in a Tubular Reactor显示文摘With the help of the ceramic foam research efforts and preparation techniques, the ZrO2 polycrystalline ceramic foam catalyst was synthesized, and its characteristics, including the crystal structure, the phase composition, the acid–base properties, and the microstructure, were analyzed by XRD, SEM, Py-IR, and BET techniques. The performance of the ZrO2 polycrystalline ceramic foam catalyst in a tubular reactor was investigated via biodiesel synthesis using S. wilsoniana oil and methanol. The effects of reaction conditions(i.e., reaction temperature, reaction pressure, and volume ratio of methanol to S. wilsoniana oil) on transesterification efficiency were investigated, and the reaction conditions were optimized using RSM. The optimum reaction temperature, reaction pressure, and volume ratio of methanol to S. wilsoniana oil were determined to be 290 ℃, 10 MPa, and 4:1, respectively. Under this condition, the FAME content in the product oil reached 98.38%. The performance of the ZrO2 polycrystalline ceramic foam catalyst synthesized in this work for biodiesel synthesis from S. wilsoniana oil with a moisture content of 7.1% and an acid value of 130.697 mg KOH/g was examined, and the FAME content in the product oil was found to be 93% and 97.67%, respectively. The FAME content in the product oil exceeded 97% after five consecutive cycles(12 h per cycle of use) of the catalyst. The proposed catalyst represents a new type of solid catalyst with excellent acid resistance, water resistance, esterification efficiency, and catalytic stability. | Wang Yunpu Fan Liangliang Dai Leilei Liu Yuhuan Ruan Roger Liu Shitao Zhang Xueqin Wan Yiqin | 2015 | China Petroleum Processing & Petrochemical Technology2015，17，3： | 3 |
| 15 | Carpel of cucumber (Cucumis sativus. L) male flowers maintains early primordia characteristics during organ development显示文摘Diclinism is not only an interesting natural phenomenon in plant kingdom, but also of significance in the yield of crops, e.g. cucumber and watermelon. Cucumber has been a model plant for studying the diclinism for decades. However, it is stili obscure how the carpel primordia of cucumber male flower undergo the developmental processes after the initiation of their development. By showing morphological alternation, metabolic activity, as well as CFL gene expression, the present study demonstrated that the carpel primordia of cucumber male flowers maintained the characteristics of primordium until anthesis, although it developed significantly slower than that of the female flowers. | YANG Lingling CHEN Min LIU Fuquan GENG Yi CHEN Chong LI Yiqin CAO Zongxun XU Zhihong BAI Shunong | 2000 | Chinese Science Bulletin2000，45，8： | 3 |
| 16 | 3D Printed Ultrastretchable,Hyper-Antifreezing Conductive Hydrogel for Sensitive Motion and Electrophysiological Signal Monitoring显示文摘Conductive hydrogels with high stretchability can extend their applications as a flexible electrode in electronics,biomedicine,human-machine interfaces,and sensors.However,their time-consuming fabrication and narrow ranges of working temperature and working voltage severely limit their further potential applications.Herein,a conductive nanocomposite network hydrogel fabricated by projection microstereolithography(PμSL)based 3D printing is proposed,enabling fast fabrication ability with high precision.The 3D printed hydrogels exhibit ultra-stretchability(2500%),hyper-antifreezing(-125℃),extremely low working voltage(<100μV),and super cyclic tensile stability(1 million cycles).The hydrogel-based strain sensor can probe both large-scale and tiny human motions,even with ultralow voltage of 100μV at extremely low temperature around-115℃.It is demonstrated that the present hydrogels can be used as a flexible electrode for capturing human electrophysiological signals(EOG and EEG),where the alpha and beta waves from the brain can be recorded precisely.Therefore,the present hydrogels will pave the way for the development of next-generation intelligent electronics,especially for those working under extremely lowtemperature environments. | Zhaolong Wang Lei Chen Yiqin Chen Peng Liu Huigao Duan Ping Cheng | 2020 | Research2020，，1： | 2 |
| 17 | On the molecular mechanism of ion specific Hofmeister series显示文摘Hofmeister series ranks the ability of salt ions in influencing a variety of properties and processes in aqueous solutions.In this review,we reexamine how these ions and some other small molecules affect water structure and thermodynamic properties,such as surface tension and protein backbone solvation.We illustrate the difficulties in interpreting the thermodynamic information based on structural and dynamic arguments.As an alternative,we show that the solvation properties of ions and proteins/small molecules can be used to explain the salt effects on the thermodynamic properties of the solutions.Our analysis shows that the often neglected cation-anion cooperativity plays a very important role in these effects.We also argue that the change of hydrogen donor/acceptor equilibrium by added cosolutes/cosolvents can be used to explain their effects on protein secondary structure denaturation/protection:those increase hydrogen donor concentrations such as urea and salts with strongly solvated cations/weakly hydrated anions tend to dissolve protein backbone acting as secondary structure denaturants,whereas those lack of hydrogen donors but rich in acceptors have the opposite effect. | XIE WenJun LIU ChengWen YANG LiJiang GAO YiQin | 2014 | Science China Chemistry2014，57，1： | 2 |
| 18 | Kirigami-inspired multiscale patterning of metallic structures via predefined nanotrench templates显示文摘Reliable fabrication of multiscale metallic patterns with precise geometry and size at both the nanoscale and macroscale is of importance for various applications in electronic and optical devices.The existing fabrication processes,which usually involve film deposition in combination with electron-beam patterning,are either timeconsuming or offer limited precision.Inspired by the kirigami,an ancient handicraft art of paper cutting,this work demonstrates an electron-beam patterning process for multiscale metallic structures with significantly enhanced efficiency and precision.Similar to the kirigami,in which the final pattern is defined by cutting its contour in a paper and then removing the unwanted parts,we define the target multiscale structures by first creating nanotrench contours in a metallic film via an electron-beam-based process and then selectively peeling the separated film outside the contours.Compared with the conventional approach,which requires the exposure of the whole pattern,much less exposure area is needed for nanotrench contours,thus enabling reduced exposure time and enhanced geometric precision due to the mitigated proximity effect.A theoretical model based on interface mechanics allows a clear understanding of the nanotrench-assisted selective debonding behaviour in the peeling process.By using this fabrication process,multiscale metallic structures with sub-10-nm up to submillimetre features can be reliably achieved,having potential applications for anti-counterfeiting and gap-plasmon-enhanced spectroscopy. | Mengjie Zheng Yiqin Chen Zhi Liu Yuan Liu Yasi Wang Peng Liu Qing Liu Kaixi Bi Zhiwen Shu Yihui Zhang Huigao Duan | 2019 | Microsystems & Nanoengineering2019，5，1： | 2 |
| 19 | Molecular Mechanism of Induction on Apoptosis of Human Esophageal Cancer HCE-4 Cells by Active Components from Astragalus membranaceus显示文摘[Objectives] To investigate the pharmacologic effects of active components from A. membranaceus on human esophageal cancer HCE-4 cells and its apoptosis mechanism. [Methods] The viabilities of HCE-4 cells were measured by MTT assay. The induction of active components from A. membranaceus on apoptosis of HCE-4 cells was detected by Annexin V-FITC/PI double staining. The apoptotic-related protein expression levels were determined by Western blotting. [Results] Formononetin and astragaloside IV suppressed the proliferation of HCE-4 cells in a dose-dependent manner. The Annexin V-FITC/PI double staining results showed that formononetin and astragaloside IV could induce HCE-4 cells apoptosis in a time-dependent manner. The Western blotting results showed that formononetin and astragaloside IV could significantly down-regulate p-AKT,pro-caspase-3,and increase cle-caspase-3 protein expression in HCE-4 cells. [Conclusions]Active components from A. membranaceus such as formononetin and astragaloside IV significantly inhibited the proliferation of human esophageal cancer HCE-4 cells by inducing mitochondrial dependent apoptosis via AKT signaling pathway. | Jiaru WANG Yinghua LUO Xianji PIAO Chang LIU Yi ZHANG Hao WANG Jinqian LI Wanting XU Yang LIU Yiqin WU Chenghao JIN | 2018 | Medicinal Plant2018，9，1： | 2 |
| 20 | Coordinated regulation of active and repressive histone methylations by a dual-specificity histone demethylase ceKDM7A from Caenorhabditis elegans显示文摘H3K9me2 和 H3K27me2 是与抄写压抑联系的重要 epigenetic 标记,当 H3K4me3 与抄写激活被联系时。活跃、压抑的 histone methylations 以一种互相独占的方式散布,这被显示出,但是内在的机制糟糕被理解。这里,我们识别了 ceKDM7A,一个哲学博士(植物 homeodomain )- 并且 JmjC 包含域的蛋白质,为 H3K9me2 和 H3K27me2 特定的 histone demethylase。我们进一步证明 ceKDM7A 的 PHD 领域在染色体宽的水平绑了 H3K4me3 和与 ceKDM7A co 局部性的 H3K4me3。到 H3K4me3 的 PHD 域绑定的混乱在 vivo 减少了 demethylase 活动,并且 ceKDM7A 的损失减少了它的联系目标基因的表达式。这些结果显示 ceKDM7A 被招募到倡导者到 demethylate H3K9me2 和 H3K27me2 并且通过 PHD 领域的绑定激活基因表示到 H3K4me3。因此,我们的学习识别双特性的 histone demethylase 并且提供新奇卓见进 histone methylation 的规定。 | Hanqing Lin Yiqin Wang Yanru Wang Feng Tian Pu Pu Yi Yu Hailei Mao Ying Yang Ping Wang Lulu Hu Yan Lin Yi Liu Yanhui Xu Charlie Degui Chen | 2010 | Cell Research2010，20，8： | 2 |
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