|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Mechanism of floral scent production in Osmanthus fragrans and the production and regulation of its key floral constituents,β-ionone and linalool显示文摘Sweet osmanthus(Osmanthus fragrans Lour.)is among the top ten most well-known flowers in China and is recognized as both an aromatic plant and ornamental flower.Here,manual sectioning,scanning electron microscopy,and transmission electron microscopy of sweet osmanthus petals revealed that large amounts of lipids are present inside the petal cells and on the cell surfaces.However,no secretory structures were observed.Instead,the petal cells protrude slightly outward,and the surfaces of the cells are adorned with highly regular brush-shaped hairs.The surfaces of the‘Yingui’petals possessed mostly curled and more numerous hairs,whereas the‘Dangui’petals possessed fewer brush-shaped and more sparsely arranged hairs.In addition,many granular substances were attached to the brush-shaped hairs,and the granules were denser on the hairs of the‘Yingui’petals compared to the hairs on the‘Dangui’petals.Furthermore,35 aromatic components in the‘Yingui’petals and 30 aromatic components in the‘Dangui’petals were detected via GC-MS.The main aromatic component of the‘Yingui’petals wasβ-ionone,whereas that of the‘Dangui’petals was linalool and its oxides.Transcriptome sequencing and qRT-PCR indicated that the highβ-ionone content in the‘Yingui’petals was due to the overexpression of CCD1 and CCD4 and that the high linalool content in the‘Dangui’petals was due to the overexpression of MECS,HDR,IDI1,and LIS1,which function upstream of the linalool synthetic pathway.In particular,the expression levels of CCD4 and LIS1 were upregulated by 5.5-and 5.1-fold in the‘Yingui’and‘Dangui’petals,respectively.One transcription factor(ERF61)was cloned and named,and the expression pattern of ERF61 in sweet osmanthus petals was found to be generally consistent with that of CCD4.Tobacco transformation experiments,yeast one-hybrid experiments,and electrophoretic mobility shift assays indicated that ERF61 binds to the CCD4 promoter and stimulates CCD4 expression,thereby regulating the synthesis ofβ-ionone in sweet osmanthus petals. | Yuanji Han Hongyun Wang Xiaodan Wang Ke Li Meifang Dong Yong Li Qian Zhu Fude Shang | 2019 | Horticulture Research2019，6，1： | 13 |
| 2 | Antibiotics-mediated intestinal microbiome perturbation aggravates tacrolimus-induced glucose disorders in mice显示文摘Both immunosuppressants and antibiotics (ABX) are indispensable for transplant patients. However, the former increases the risk of new-onset diabetes, whereas the latter impacts intestinal microbiota (IM). It is still unclear whether and how the interaction between immunosuppressants and ABX alters the IM and thus leads to glucose metabolism disorders. This study examined the alterations of glucose and lipid metabolism and IM in mice exposed to tacrolimus (TAC) with or without ABX. We found that ABX further aggravated TAC-induced glucose tolerance and increased insulin secretion. Combined treatment resulted in exacerbated lipid accumulation in the liver. TAC-altered microbial community was further amplified by ABX administration, as characterized by reductions in phylum Firmicutes, family Lachnospiraceae, and genus Copyococcus. Analyses based on the metagenomic profiles revealed that ABX augmented the effect of TAC on microbial metabolic function mostly related to lipid metabolism. The altered components of gut microbiome and predicted microbial functional profiles showed significant correlation with hepatic lipid accumulation and glucose disorders. In conclusion, ABX aggravated the effect of TAC on the microbiome and its metabolic capacities, which might contribute to hepatic lipid accumulation and glucose disorders. These findings suggest that the ABX-altered microbiome can amplify the diabetogenic effect of TAC and could be a novel therapeutic target for patients. | Yuqiu Han Xiangyang Jiang Qi Ling Li Wu Pin Wu Ruiqi Tang Xiaowei Xu Meifang Yang Lijiang Zhang Weiwei Zhu Baohong Wang Lanjuan Li | 2019 | Frontiers of Medicine2019，13，4： | 3 |
| 3 | Crosslinking and functionalization of acellular patches via the self-assembly of copper@tea polyphenol nanoparticles显示文摘Decellularization is a promising technique to produce natural scaffolds for tissue engineering applications.However,non-crosslinked natural scaffolds disfavor application in cardiovascular surgery due to poor biomechanics and rapid degradation.Herein,we proposed a green strategy to crosslink and functionalize acellular scaffolds via the self-assembly of copper@tea polyphenol nanoparticles(Cu@TP NPs),and the resultant nanocomposite acellular scaffolds were named as Cu@TP-dBPs.The crosslinking degree,biomechanics,denaturation temperature and resistance to enzymatic degradation of Cu@TP-dBPs were comparable to those of glutaraldehyde crosslinked decellularized bovine pericardias(Glut-dBPs).Furthermore,Cu@TP-dBPs were biocompatible and had abilities to inhibit bacterial growth and promote the formation of capillary-like networks.Subcutaneous implantation models demonstrated that Cu@TP-dBPs were free of calcification and allowed for host cell infiltration at Day 21.Cardiac patch graft models confirmed that Cu@TP-dBP patches showed improved ingrowth of functional blood vessels and remodeling of extracellular matrix at Day 60.These results suggested that Cu@TP-dBPs not only had comparable biomechanics and biostability to Glut-dBPs,but also had several advantages over Glut-dBPs in terms of anticalcification,remodeling and integration capabilities.Particularly,they were functional patches possessing antibacterial and proangiogenic activities.Thesematerial properties and biological functionsmade Cu@TP-dBPs a promising functional acellular patch for cardiovascular applications. | Qin Li Yuan Gao Jiajun Zhang Yangfeng Tang Yangyong Sun Lujia Wu Hao Wu Meifang Shen Xiaohong Liu Lin Han Zhiyun Xu | 2022 | Regenerative Biomaterials2022，9，1： | 1 |
| 4 | The mechanism underlying extrapulmonary complications of the coronavirus disease 2019 and its therapeutic implication显示文摘The coronavirus disease 2019(COVID-19)is a highly transmissible disease caused by the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)that poses a major threat to global public health.Although COVID-19 primarily affects the respiratory system,causing severe pneumonia and acute respiratory distress syndrome in severe cases,it can also result in multiple extrapulmonary complications. | Qin Ning Di Wu Xiaojing Wang Dong Xi Tao Chen Guang Chen Hongwu Wang Huiling Lu Ming Wang Lin Zhu Junjian Hu Tingting Liu Ke Ma Meifang Han Xiaoping Luo | 2022 | Signal Transduction and Targeted Therapy2022，7，3： | 1 |
| 5 | Assessment of genetic diversity and relationships among Osmanthus fragrans cultivars using AFLP markers显示文摘 | Yuan Wangjun Han Yuanji Dong Meifang | 2011 | Electronic Journal of Biotech- nology2011，14，1： | 1 |
| 6 | A TEMPOL and rapamycin loaded nanofiber-covered stent favors endothelialization and mitigates neointimal hyperplasia and local inflammation显示文摘An increased level of reactive oxygen species(ROS)plays a major role in endothelial dysfunction and vascular smooth muscle cell(VSMC)proliferation during in-stent thrombosis and restenosis after coronary artery stenting.Herein,we report an electrospun core-shell nanofiber coloaded with 4-hydroxy-2,2,6,6-tetramethylpiperidine 1-oxyl(TEMPOL)and rapamycin(RAPA)that correspondingly serves as an ROS scavenger and VSMC inhibitor.This system has the potential to improve the biocompatibility of current drug-eluting stent(DES)coatings with the long-term and continuous release of TEMPOL and rapamycin.Moreover,the RAPA/TEMPOL-loaded membrane selectively inhibited the proliferation of VSMCs while sparing endothelial cells(ECs).This membrane demonstrated superior ROS-scavenging,anti-inflammatory and antithrombogenic effects in ECs.In addition,the membrane could maintain the contractile phenotype and mitigate platelet-derived growth factor BB(PDGF-BB)-induced proliferation of VSMCs.In vivo results further revealed that the RAPA/TEMPOL-loaded covered stents promoted rapid restoration of vascular endothelium compared with DES and persistently impeded inflammation and neointimal hyperplasia in porcine models. | Rui Wang Jian Lu Jiasheng Yin Han Chen Hongmei Liu Fei Xu Tongtong Zang Rende Xu Chenguang Li Yizhe Wu Qilin Wu Xiang Fei Meifang Zhu Li Shen Junbo Ge | 2023 | Bioactive Materials2023，，1： | 1 |
| 7 | Temporally Separated CO_(2) Photoreduction and H_(2)O Photooxidation over Redox-Active Conjugated Organic Polymer Films显示文摘Photocatalytic CO_(2) reduction with H_(2)O of chemicals without H2 generation is interesting but challenging.Herein,we report temporally separated CO_(2) photoreduction and H_(2)O photooxidation that are achieved over redox-active o-hydroxyazo-based conjugated organic polymer films(HAzo-COPFs),affording CO with high efficiency as the sole reduction product.HAzo-COPFs are prepared via interfacial diazo-coupling reactions of aromatic diamines and diphenols,and HAzo-COPF-1 from 4,4′-biphenol and benzidine shows the best performance with a CO generation rate of 53.6μmol g−1 h−1 under visible-light irradiation(>420 nm).Interestingly,we discovered that diphenol(DP-OH)moieties in HAzo-COPFs,serving as electron and proton donors to participate in CO_(2) photoreduction,are oxidized into quinone(DP=O)moieties,which are subsequently photoreduced to regenerate DP-OH in H_(2)O photooxidation.Consequently,CO_(2) photoreduction and H_(2)O photooxidation are temporally separated and perfectly coupled via redox transformation between DP-OH and DP=O that form in situ,affording enhanced charge carrier separation and inhibiting the hydrogen evolution reaction.This work provides new insights for the design of COP photocatalysts and artificial photosynthesis. | Guipeng Ji Yuepeng Wang Wenqiang Ye Meifang Chen Fengtao Zhang Yanfei Zhao Shengrui Tong Buxing Han Zhimin Liu | 2023 | CCS Chemistry2023，5，8： | 0 |
| 8 | Evaluation of hydrogels for soft tissue adhesives in vitro and in vivo analyses显示文摘 | Liu YUAN Wenshuai FAN Linyingjun HAN Changan GUO Zuoqin YAN Meifang ZHU Xiumei MO | 2018 | Frontiers of Materials Science2018，12，1： | 0 |
| 9 | Upgraded antisolvent engineering enables 2D@3D quasi core-shell perovskite for achieving stable and 21.6%efficiency solar cells显示文摘Perovskite solar cells(PSCs)have become a promising alternative to sustainable energy due to their high power conversion efficiency(PCE)and low-cost processing.However,the practical applications of PSCs are still limited by the trade-off between high performance and poor stability under operation.Here,a2D@3D perovskite with quasi core-shell architecture linking the superiorities of both two-dimensional(2D)and three-dimensional(3D)perovskite is prepared through a novel upgraded antisolvent approach.The basic properties as well as the phase distribution and the charge transport behavior of the 2D@3D perovskite were systematically elucidated.A high PCE of 21.60%for 2D@3D PSCs is achieved due to the enhanced surface and grain boundaries passivation,improved energy level alignment and efficient holes transport.The 2D@3D perovskite device without encapsulation shows significantly improved stability at the room temperature(90%of initial PCE for 45 d with a relative humidity of 50%±5%)and relative thermal conditions(83%of initial PCE for 200 h under 85℃).Compared with traditional 3D PSCs,it proved that such 2D@3D perovskite configuration is an effective architecture for enhancing efficiency and improving stability and therefore will facilitate the further industrialization of PSCs. | Fengyou Wang Jinyue Du Yuhong Zhang Meifang Yang Donglai Han Lili Yang Lin Fan Yingrui Sui Yunfei Sun Jinghai Yang | 2021 | Journal of Materials Science & Technology2021，，33： | 0 |
| 10 | Biocompatible and antibacterial soy protein isolate/quaternized chitosan composite sponges for acute upper gastrointestinal hemostasis显示文摘Innovative biomedical applications have high requirements for biomedical materials.Herein,a series of biocompatible,antibacterial and hemostatic sponges were successfully fabricated for the treatment of acute upper gastrointestinal bleeding(AUGB).Quaternized chitosan(QC)and soy protein isolate(SPI)were chemically cross-linked to obtain porous SPI/QC sponges(named SQS-n,with n¼30,40,50 or 60 corresponding to the weight percentage of the QC content).The chemical composition,physical properties and biological activity of SQS-n were investigated.SQS-n could support the adhesion and proliferation of L929 cells while triggering no obvious blood toxicity.Meanwhile,SQS-n exhibited good broad-spectrum antibacterial activity against both grampositive bacteria(Staphylococcus aureus)and gram-negative bacteria(Escherichia coli).The in vivo hemostatic effect of SQS-n was evaluated using three different bleeding models.The results revealed that SQS-50 performed best in reducing blood loss and hemostatic time.The overall hemostatic effect of SQS-50 was comparable to that of a commercial gelatin sponge.The enhanced antibacterial and hemostatic activities of SQS-n were mainly attributed to the QC component.In conclusion,this work developed a QC-functionalized hemostatic sponge that is highly desirable for innovative biomedical applications,such as AUGB. | Zijian Wang MeiFang Ke Liu He Qi Dong Xiao Liang Jun Rao Junjie Ai Chuan Tian Xinwei Han Yanan Zhao | 2021 | Regenerative Biomaterials2021，8，4： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员