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| 1 | Potentiating CD8^(+) T cell antitumor activity by inhibiting PCSK9 to promote LDLR-mediated TCR recycling and signaling显示文摘Metabolic regulation has been proven to play a critical role in T cell antitumor immunity.However,cholesterol metabolism as a key component of this regulation remains largely unexplored.Herein,we found that the low-density lipoprotein receptor(LDLR),which has been previously identified as a transporter for cholesterol,plays a pivotal role in regulating CD8+T cell antitumor activity.Besides the involvement of cholesterol uptake which is mediated by LDLR in T cell priming and clonal expansion,we also found a non-canonical function of LDLR in CD8+T cells:LDLR interacts with the T-cell receptor(TCR)complex and regulates TCR recycling and signaling,thus facilitating the effector function of cytotoxic T-lymphocytes(CTLs).Furthermore,we found that the tumor microenvironment(TME)downregulates CD8+T cell LDLR level and TCR signaling via tumor cell-derived proprotein convertase subtilisin/kexin type 9(PCSK9)which binds to LDLR and prevents the recycling of LDLR and TCR to the plasma membrane thus inhibits the effector function of CTLs.Moreover,genetic deletion or pharmacological inhibition of PCSK9 in tumor cells can enhance the antitumor activity of CD8+T cells by alleviating the suppressive effect on CD8+T cells and consequently inhibit tumor progression.While previously established as a hypercholesterolemia target,this study highlights PCSK9/LDLR as a potential target for cancer immunotherapy as well. | Juanjuan Yuan Ting Cai Xiaojun Zheng Yangzi Ren Jingwen Qi Xiaofei Lu Huihui Chen Huizhen Lin Zijie Chen Mengnan Liu Shangwen He Qijun Chen Siyang Feng Yingjun Wu Zhenhai Zhang Yanqing Ding Wei Yang | 2021 | Protein & Cell2021，12，4： | 12 |
| 2 | Crystal clear: visualizing the intervention mechanism of the PD-1/PD-L1 interaction by two cancer therapeutic monoclonal antibodies显示文摘基于抗体的 PD-1/PD-L1 封锁治疗为癌症在免疫疗法拿了中心舞台,与多重临床的成功。发信号的 PD-1 在肿瘤驱动的 T 房间机能障碍起枢轴的作用。与优先的途径相对照产生或增加肿瘤特定的 T 房间回答,基于抗体的 PD-1/PD-L1 封锁指向导致肿瘤的 T 房间缺点并且恢复先存在调制 antitumor 免疫的 T 房间功能。在这评论,表达式规定和 PD-1 的禁止的函数和基于抗体的 PD-1/PD-L1 封锁治疗的现在的理解上的基本知识简短被总结。我们然后有关 PD-1/PD-Ls 相互作用的结构的基础集中于最近的突破工作并且怎么指向 PD-L1 的治疗学的抗体, pembrolizumab 指向 PD-1 和 avelumab,与 PD-1/PD-L1 的绑定竞争打断 PD-1/PD-L1 相互作用。我们相信这个结构的信息将有益于指向 PD-1 发信号的治疗学的抗体的设计和改进。 | ShuguangTan Danqing Chen Kefang Liu Mengnan He Hao Song Yi Shi Jun Liu Catherine W.-H. Zhang Jianxun Qi Jinghua Yan Shan Gao George F. Gao | 2016 | Protein & Cell2016，7，12： | 8 |
| 3 | Procalcitonin could be a reliable marker in differential diagnosis of post-implantation syndrome and infection after percutaneous endovascular aortic repair显示文摘 | Xue Ling Luo Songyuan Luo Jianfang Liu Zhen Gu Mengnan Kang Huiyuan Yang Fan Nie Bingrong Liu Yuan Huang Wenhui Xie Nianjin He Pengcheng Dong Haojian Ni Zhonghan Fan Ruixin Chen Jiyan | 2014 | Chinese Medical Journal2014，，14： | 8 |
| 4 | Two-dimensional self-healing hydrogen-bond-based supramolecula polymer film显示文摘Self-healing materials have been developed over the past decade with the recovery ability after damage.However,most researches focused on the self-healing process at three-dimension.Herein,we prepare monolayer self-healing hydrogen-bond-based supramolecular polymer film and explore the self-healing process at the two-dimensional limit.The healing process,which can be reversibly repeated for at least three times,is influenced by the temperature,the molecule-substrate interaction and the substrate roughness.In the application,the monolayer self-healing polymer film can be used to modify the SiO2 dielectric for copper phthalocyanine field effect transistor with improved mobility.This work will be valuable for developing two-dimensional functional self-healing materials in the future. | Mengnan He Xiaosong Chen Donghua Liu Dacheng Wei | 2019 | Chinese Chemical Letters2019，30，5： | 5 |
| 5 | Generation and characterization of stable pig pregastrulation epiblast stem cell lines显示文摘Pig epiblast-derived pluripotent stem cells are considered to have great potential and broad prospects for human therapeutic model developme nt and livestock breeding.Despite on going attempts since the 1990s,no stably defined pig epiblast-derived stem cell line has bee n established.Here,guided by in sights from a large-scale sin gle-cell tran scriptome an alysis of pig embryos from embryonic day(E)0 to E14,specifically,the tracing of pluripotency changes during epiblast development,we developed an in vitro culture medium for establishing and maintaining stable pluripotent stem cell lines from pig E10 pregastrulation epiblasts(pgEpiSCs).Enabled by chemical inhibition of WNT-related signaling in combination with growth factors in the FGF/ERK,JAK/STAT3,and Activin/Nodal pathways,pgEpiSCs maintain their pluripotency transcriptome features,similar to those of E10 epiblast cells,and normal karyotypes after more than 240 passages and have the potential to differentiate into three germ layers.Strikingly,ultradeep in situ Hi-C analysis revealed functional impacts of chromatin 3D-spatial associations on the transcriptional regulation of pluripote ncy marker genes in pgEpiSCs.I n practice,we con firmed that pgEpiSCs readily tolerate at least three rounds of successive gene editi ng and gene rated cloned gen e-edited live piglets.Our findings deliver on the long-a nticipated promise of pig pluripote nt stem cells and open new avenues for biological research,animal husbandry,and regenerative biomedicine. | Minglei Zhi Jinying Zhang Qianzi Tang Dawei Yu Shuai Gao Dengfeng Gao Pengliang Liu Jianxiong Guo Tang Hai Jie Gao Suying Cao Zimo Zhao Chongyang Li Xiaogang Weng Mengnan He Tianzhi Chen Yingjie Wang Keren Long Deling Jiao Guanglei Li Jiaman Zhang Yan Liu Yu Lin Daxin Pang Qianqian Zhu Naixin Chen Jingjing Huang Xinze Chen Yixuan Yao Jingcang Yang Zicong Xie Xianya Huang Mengxin Liu Ran Zhang Qiuyan Li Yiliang Miao Jianhui Tian Xingxu Huang Hongsheng Ouyang Bofeng Liu Wei Xie Qi Zhou Zhonghua Liu Caihong Zheng Mingzhou Li Jianyong Han | 2022 | Cell Research2022，32，4： | 3 |
| 6 | A New Gene Conferring the Glabrous Trait in Cucumber Identified Using MutMap显示文摘Tubercles and spines on fruit peel are important commercial traits in cucumber(Cucumis sativus L.). From an ethyl methane sulfonate cucumber mutant library,we discovered a new glabrous mutant that bears no tubercle or spine on fruit peel and fewer and smaller trichomes on the stem and leaves. The new locus is here designated as glabrous2(gl2). Genome sequencing of the mutant and linkage analysis revealed that a non-synonymous mutation in the Csa1G056960 gene rendered the gl2 phenotype. The mutated gene encodes a C-type lectin receptor-like tyrosine protein kinase. This study provides a novel allele for elucidating the genetic basis of wart and trichome development and a new tool for breeding glabrous cucumber varieties. | XU Mengnan WANG Shenhao ZHANG Shu CUI Qingzhi GAO Dongli CHEN Huiming HUANG Sanwen | 2015 | Horticultural Plant Journal2015，1，1： | 2 |
| 7 | An invasive zone in human liver cancer identified by Stereo-seq promotes hepatocyte–tumor cell crosstalk,local immunosuppression and tumor progression显示文摘Dissecting and understanding the cancer ecosystem,especially that around the tumor margins,which have strong implications for tumor cell infiltration and invasion,are essential for exploring the mechanisms of tumor metastasis and developing effective new treatments.Using a novel tumor border scanning and digitization model enabled by nanoscale resolution-SpaTial Enhanced REsolution Omics-sequencing(Stereo-seq),we identified a 500µm-wide zone centered around the tumor border in patients with liver cancer,referred to as“the invasive zone”.We detected strong immunosuppression,metabolic reprogramming,and severely damaged hepatocytes in this zone.We also identified a subpopulation of damaged hepatocytes with increased expression of serum amyloid A1 and A2(referred to collectively as SAAs)located close to the border on the paratumor side.Overexpression of CXCL6 in adjacent malignant cells could induce activation of the JAK-STAT3 pathway in nearby hepatocytes,which subsequently caused SAAs’overexpression in these hepatocytes.Furthermore,overexpression and secretion of SAAs by hepatocytes in the invasive zone could lead to the recruitment of macrophages and M2 polarization,further promoting local immunosuppression,potentially resulting in tumor progression.Clinical association analysis in additional five independent cohorts of patients with primary and secondary liver cancer(n=423)showed that patients with overexpression of SAAs in the invasive zone had a worse prognosis.Further in vivo experiments using mouse liver tumor models in situ confirmed that the knockdown of genes encoding SAAs in hepatocytes decreased macrophage accumulation around the tumor border and delayed tumor growth.The identification and characterization of a novel invasive zone in human cancer patients not only add an important layer of understanding regarding the mechanisms of tumor invasion and metastasis,but may also pave the way for developing novel therapeutic strategies for advanced liver cancer and other solid tumors. | Liang Wu Jiayan Yan Yinqi Bai Feiyu Chen Xuanxuan Zou Jiangshan Xu Ao Huang Liangzhen Hou Yu Zhong Zehua Jing Qichao Yu Xiaorui Zhou Zhifeng Jiang Chunqing Wang Mengnan Cheng Yuan Ji Yingyong Hou Rongkui Luo Qinqin Li Liang Wu Jianwen Cheng Pengxiang Wang Dezhen Guo Waidong Huang Junjie Lei Shang Liu Yizhen Yan Yiling Chen Sha Liao Yuxiang Li Haixiang Sun Na Yao Xiangyu Zhang Shiyu Zhang Xi Chen Yang Yu Yao Li Fengming Liu Zheng Wang Shaolai Zhou Huanming Yang Shuang Yang Xun Xu Longqi Liu Qiang Gao Zhaoyou Tang Xiangdong Wang Jian Wang Jia Fan Shiping Liu Xinrong Yang Ao Chen Jian Zhou | 2023 | Cell Research2023，33，8： | 1 |
| 8 | Mitochondrial heterogeneity in diseases显示文摘As key organelles involved in cellular metabolism,mitochondria frequently undergo adaptive changes in morphology,components and functions in response to various environmental stresses and cellular demands.Previous studies of mitochondria research have gradually evolved,from focusing on morphological change analysis to systematic multiomics,thereby revealing the mitochondrial variation between cells or within the mitochondrial population within a single cell.The phenomenon of mitochondrial variation features is defined as mitochondrial heterogeneity.Moreover,mitochondrial heterogeneity has been reported to influence a variety of physiological processes,including tissue homeostasis,tissue repair,immunoregulation,and tumor progression.Here,we comprehensively review the mitochondrial heterogeneity in different tissues under pathological states,involving variant features of mitochondrial DNA,RNA,protein and lipid components.Then,the mechanisms that contribute to mitochondrial heterogeneity are also summarized,such as the mutation of the mitochondrial genome and the import of mitochondrial proteins that result in the heterogeneity of mitochondrial DNA and protein components.Additionally,multiple perspectives are investigated to better comprehend the mysteries of mitochondrial heterogeneity between cells.Finally,we summarize the prospective mitochondrial heterogeneity-targeting therapies in terms of alleviating mitochondrial oxidative damage,reducing mitochondrial carbon stress and enhancing mitochondrial biogenesis to relieve various pathological conditions.The possibility of recent technological advances in targeted mitochondrial gene editing is also discussed. | Long Chen Mengnan Zhou Hao Li Delin Liu Peng Liao Yao Zong Changqing Zhang Weiguo Zou Junjie Gao | 2023 | Signal Transduction and Targeted Therapy2023，8，9： | 0 |
| 9 | RIS:Spatial-Wideband Effect Analysis and Off-Grid Channel Estimation显示文摘As a critical candidate technology for 5G-advanced and 6G,reconfigurable intelligent surfaces(RIS)have received extensive atten-tion from academia and industry.RIS has the promising features of passiveness,reconfigurable ability,and low cost.RIS channel estimation faces the challenges of high matrix dimension,passive estimation,and spatial-wideband effect.In this article,we analyze the impact of the spatial-wideband effect on the RIS channel to account for the propagation delay across RIS elements and estimate sparse channel parameters such as angle and gain through a super-resolution compressive sensing(CS)algorithm.The simulation results explore the influence of the spatial-wideband effect on the RIS channel and verify the effectiveness of the proposed algorithm. | JIAN Mengnan ZHANG Nan CHEN Yijian | 2022 | ZTE Communications2022，20，1： | 0 |
| 10 | Salient object detection via region contrast and graph regularization显示文摘Detection of salient objects in an image is now gaining increasing research interest in computer vision community. In this study, a novel region-contrast based saliency detection solution involving three phases is proposed. First, a color-based super-pixels segmentation approach is used to decompose the image into regions. Second, three high-level saliency measures which could effectively characterize the salient regions are evaluated and integrated in an effective manner to produce the initial saliency map. Finally, we construct a pairwise graphical model to encourage that adjacent image regions with similar features take continuous saliency values, thus producing the more perceptually consistent saliency map. We extensively evaluate the proposed method on three public benchmark datasets, and show it can produce promising results when compared to 14state-of-the-art salient object detection approaches. | Xingming WU Mengnan DU Weihai CHEN Jianhua WANG | 2016 | Science China(Information Sciences)2016，59，3： | 0 |
| 11 | Correction to:Potentiating CD8^(+)T cell antitumor activity by inhibiting PCSK9 to promote LDLR-mediated TCR recycling and signaling显示文摘Figure 1.LDLR deficiency hinders the antitumor activity of CD8^(+)T cells.(A)Transcriptional level of genes involved in cholesterol transport in naïve CD8^(+)T cells,CTLs and CD8^(+)TILs(isolated at Day3 or Day7 post CTLs adoptive transfer),(n=4).(B)LDLR expression level on CTLs and CD8^(+)TILs(isolated at Day3 post CTLs adoptive transfer),(n=4). | Juanjuan Yuan Ting Cai Xiaojun Zheng Yangzi Ren Jingwen Qi Xiaofei Lu Huihui Chen Huizhen Lin Zijie Chen Mengnan Liu Shangwen He Qijun Chen Siyang Feng Yingjun Wu Zhenhai Zhang Yanqing Ding Wei Yang | 2022 | Protein & Cell2022，13，9： | 0 |
| 12 | Degree of Freedom Analysis for Holographic MIMO Based on a Mutual-Coupling-Compliant Channel Model显示文摘Degree of freedom(DOF)is a key indicator for spatial multiplexing layers of a wireless channel.Traditionally,the channel of a multiple-input multiple-output(MIMO)half-wavelength dipole array has a DOF that equals the antenna number.However,recent studies suggest that the DOF could be less than the antenna number when strong mutual coupling is considered.We utilize a mutual-coupling-compliant channel model to investigate the DOF of the holographic MIMO(HMIMO)channel and give a upper bound of the DOF with strong mutual coupling.Our numerical simulations demonstrate that a dense array can support more DOF per unit aperture as compared with a half-wavelength MIMO system. | SUN Yunqi JIAN Mengnan YANG Jun ZHAO Yajun CHEN Yijian | 2024 | ZTE Communications2024，22，1： | 0 |
| 13 | Papillary fibroblast-recruiting injectable self-healing multifunctional hydrogels for wound regeneration显示文摘The recruitment of key cells to regeneration sites is a promising strategy to promote functional wound healing.Dermal fibroblasts exhibit a heterogeneous population of cells during homeostasis and in response to injury.Papillary fibroblasts play central regulatory roles in the regeneration of skin appendages during wound healing.Inspired by the phenomenon where bait for grass carp can attract grouped grass carps to a fishing spot soon,“Grass Carp Fishing”multifunctional hydrogels,that is,codelivery of an antibody of leucine-rich repeats and immunoglobulin-like domains 1(Lrig1)on the surface of papillary fibroblasts and insulinlike growth factor 1(IGF1)with recruitment function,can recruit papillary fibroblasts.In the experiments,carboxymethyl chitosan showed positive effects in promoting cell proliferation and neovascularization,while dopamine-grafted gelatin was effective in stabilizing the structure and prolonging the degradation time.The sustained release of Lrig1 antibodies and IGF1 from injectable self-healing multifunctional hydrogels persistently accelerated the migration and proliferation of Lrig1+fibroblasts.The in vivo results from a full-thickness cutaneous wound model showed that injectable self-healing multifunctional hydrogel accelerated wound healing and skin regeneration through the recruitment of Lrig1+papillary fibroblasts in wound tissue.Our findings reveal an injectable self-healing multifunctional hydrogel for regeneration,a promising approach to promoting skin wound healing. | Yueyue Li Weifeng Zhong Junjie Wu Yuan Jia Zhonghua Chen Kaiwen Zhang Danyang Guo Yifei Fu Mengnan Chen Weiwei Chen Fan Tian Xia Li Hongji Zhang Xiaojin Zhou Feng-Lai Yuan | 2023 | Nano Research2023，16，7： | 0 |
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