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| 1 | Expenditure and financial burden for the diagnosis and treatment of colorectal cancer in China:a hospital.based,multicenter,cross-sectional survey显示文摘Background: The increasing prevalence of colorectal cancer(CRC) in China and the paucity of information about relevant expenditure highlight the necessity of better understanding the financial burden and effect of CRC diagnosis and treatment. We performed a survey to quantify the direct medical and non-medical expenditure as well as the resulting financial burden of CRC patients in China.Methods: We conducted a multicenter, cross-sectional survey in 37 tertiary hospitals in 13 provinces across China between 2012 and 2014. Each enrolled patient was interviewed using a structured questionnaire. All expenditure data were inflated to the 2014 Chinese Yuan(CNY; 1 CNY = 0.163 USD). We quantified the overall expenditure and financial burden and by subgroup(hospital type, age at diagnosis, sex, education, occupation, insurance type, household income, clinical stage, pathologic type, and therapeutic regimen). We then performed generalized linear modeling to determine the factors associated with overall expenditure.Results: A total of 2356 patients with a mean age of 57.4 years were included, 57.1 % of whom were men; 13.9% of patients had stage I cancer; and the average previous-year household income was 54,525 CNY.The overall average direct expenditure per patient was estimated to be 67,408 CNY, and the expenditures for stage Ⅰ, Ⅱ, Ⅲ, and Ⅳ disease were 56,099 CNY, 59,952 CNY, 67,292 CNY, and 82,729 CNY, respectively. Non-medical expenditure accounted for 8.3%of the overall expenditure. The 1-year out-of-pocket expenditure of a newly diagnosed patient was 32,649 CNY, which accounted for 59.9% of their previous-year household income and caused 75.0% of families to suffer an unmanageable financial burden. Univariate analysis showed that financial burden and overall expenditure differed in almost all subgroups(P < 0.05), except for sex. Multivariate analysis showed that patients who were treated in specialized hospitals and those who were diagnosed with adenocarcinoma or diagnosed at a later stage were likely to spend more,whereas those with a lower household income and those who underwent surgery spent less(all P < 0.05).Conclusions: For patients in China, direct expenditure for the diagnosis and treatment of CRC seemed catastrophic,and non-medical expenditure was non-ignorable. The financial burden varied among subgroups, especially among patients with different clinical stages of disease, which suggests that, in China, CRC screening might be cost-effective. | Hui-Yao Huang Ju-Fang Shi Lan-Wei Guo Ya-Na Bai Xian-Zhen Liao Guo-iangLiu A-Yan Mao Jian-Song Ren Xiao-Jie Sun Xin-Yu Zhu Le Wang Bing-Bing Song Ling-Bin Du Lin Zhu Ji-Yong Gong Qi Zhou Yu-Qin Liu Rong Cao Ling Mai Li Lan Xiao-Hua Sun Ying Ren Jin-Yi Zhou Yuan-Zheng Wang Xiao Qi Pei-An Lou Dian Shi Ni Li Kai Zhang Jie He Min Dai | 2017 | Chinese Journal of Cancer2017，36，8： | 25 |
| 2 | The first all-season sample set for mapping global land cover with Landsat-8 data显示文摘We report the world's first all-season training and validation sample sets for global land cover classification with Landsat-8 data.Prior to this,such samples were only available at a single date primarily from the growing season.It is unknown how much limitation such a single-date sample has to mapping global land cover in other seasons of the year.To answer this question,we selected available Landsat-8 images from four seasons and collected training and validation samples from them.We compared the performances of training samples in different seasons using Random Forest algorithm.We found that the use of training samples from any individual season would result in the best overall classification accuracy when validated by samples in the same season.The global overall accuracy from combined best seasonal results was 67.2% when classifying the 11 Level-1 classes in the Finer Resolution Observation and Monitoring of Global Land Cover(FROM-GLC) classification system.The use of training samples from all seasons(named all-season training sample set hereafter) produced an overall accuracy of 67.0%.We also tested classification within 10° latitude 60° longitude zones using all-season training subsample within each zone and obtained an overall accuracy of 70.2%.This indicates that properly grouped subsamples in space can help improve classification accuracies.All the results in this study seem to suggest that it is possible to use an all-season training sample set to reach global optimality with universal applicability in classifying images acquired at any time of a year for global land cover mapping. | Congcong Li Peng Gong Jie Wang Zhiliang Zhu Gregory S. Biging Cui Yuan Tengyun Hu Haiying Zhang Qi Wang Xuecao Li Xiaoxuan Liu Yidi Xu Jing Guo Caixia Liu Kwame O. Hackman Meinan Zhang Yuqi Cheng Le Yu Jun Yang Huabing Huang Nicholas Clinton | 2017 | Science Bulletin2017，62，7： | 21 |
| 3 | Viral effector protein manipulates host hormone signaling to attract insect vectors显示文摘 | Dewei Wu Tiancong Qi Wan-Xiang Li Haixia Tian Hua Gao Jiaojiao Wang Jin Ge Ruifeng Yao Chunmei Ren Xian-Bing Wang Yule Liu Le Kang Shou-Wei Ding Daoxin Xie | 2017 | Cell Research2017，27，3： | 20 |
| 4 | Deformation characteristics and safety assessment of a high-speed railway induced by undercutting metro tunnel excavation显示文摘Stratum deformation(settlement) is a challenging issue in tunnel engineering, especially when construction of metro tunnels has to undercut high-speed railway. For this purpose, we used the FLAC30 software to analyze the stratum settlement characteristics of high-speed railway at different crossing angles intersected by metro tunnel, in terms of ground settlement trough, stratum slip line and irregularity of ballastless tracks. According to the evolution of the stratum settlement at different angle regions, an optimized angle is proposed for the actual project design. In order to reduce the influence of stratum settlement on the safety of high-speed railway, an approach of safety assessment is proposed for the shield engineering undercutting high-speed railway, as per Chinese specifications using numerical results and on-site conditions. A case study is conducted for the shield tunnel section crossing the Wuhan-Guangzhou High-speed Railway between the Guangzhou North Railway Station and the Huacheng Road Station, which represents the first metro tunnel project passing below a high-speed railway in China. A series of measures is taken to ensure the safe excavation of the shield tunnel and the operation of the high-speed railway. The results can provide a technical support for performing a safety evaluation between high-speed railways and metro tunnels. | Wangping Qian Taiyue Qi Yunjian Zhao Yizhou Le Haiyang Yi | 2019 | Journal of Rock Mechanics and Geotechnical Engineering2019，11，1： | 18 |
| 5 | Minimally invasive surgery alone compared with intensity-modulated radiotherapy for primary stage I nasopharyngeal carcinoma显示文摘Background:The National Comprehensive Cancer Network guidelines recommend intensity-modulated radiotherapy(IMRT)as the primary curative treatment for newly diagnosed nasopharyngeal carcinoma(NPC),but the radiation-related complications and relatively high medical costs remain a consequential burden for the patients.Endoscopic nasopharyngectomy(ENPG)was successfully applied in recurrent NPC with radiation free and relatively low medical costs.In this study,we examined whether ENPG could be an effective treatment for localized stage I NPC.Methods:Ten newly diagnosed localized stage I NPC patients voluntarily received ENPG alone from June 2007 to September 2017 in Sun Yat-sen University Cancer Center.Simultaneously,the data of 329 stage I NPC patients treated with IMRT were collected and used as a reference cohort.The survival outcomes,quality of life(QOL),and medical costs between two groups were compared.Results:After a median follow-up of 59.0 months(95%CI 53.4-64.6),no death,locoregional recurrence,or distant metastasis was observed in the 10 patients treated with ENPG.The 5-year overall survival,local relapse-free survival,regional relapse-free survival,and distant metastasis-free survival among the ENPG-treated patients was similar to that among the IMRT-treated patients(100%vs.99.1%,100%vs.97.7%,100%vs.99.0%,100%vs.97.4%,respectively,P>0.05).In addition,compared with IMRT,ENPG was associated with decreased total medical costs($4090.42±1502.65 vs.$12620.88±4242.65,P<0.001)and improved QOL scores including dry mouth(3.3±10.5 vs.34.4±25.8,P<0.001)and sticky saliva(3.3±10.5 vs.32.6±23.3,P<0.001).Conclusions:ENPG alone was associated with promising long-term survival outcomes,low medical costs,and satisfactory QOL and might therefore be an alternative strategy for treating newly diagnosed localized stage I NPC patients who refused radiotherapy.However,the application of ENPG should be prudent,and prospective clinical tri-als were needed to further verify the results. | You-Ping Liu Xing Lv Xiong Zou Yi-Jun Hua Rui You Qi Yang Le Xia Shao-Yan Guo Wen Hu Meng-Xia Zhang Si-Yuan Chen Mei Lin Yu-Long Xie Li-Zhi Liu Rui Sun Pei-Yu Huang Wei Fan Xiang Guo Ming-Huang Hong Ming-Yuan Chen | 2019 | Cancer Communications2019，39，1： | 17 |
| 6 | The distribution characteristics of heavy metals in Guiyang urban soils显示文摘Guiyang is located in southwestern China,which is a famous tourism city,and is awarded the title of'the Summer Capital of China'.A study on heavy metals in Guiyang urban soils was conducted.The results showed that the concentrations of Cd,Cu,Ni,Pb and Zn in Guiyang urban soils are significantly higher than their background values and Cu,Zn and Pb in Guiyang soils are accumulated seriously.The distribution characteristics of heavy metals in 3 districts are described as follows:the level of heavy metals in soils follows the order of Nanming District> Yunyan District>Xiaohe District.The spatial distribution of metals in urban soils of Guiyang shows that the level of metals in the central and eastern parts of Guiyang is relatively high,and its characteristics are attributed to the influence of commercial activities,heavy traffic and industrial emission.Compared with some other cities in China,Guiyang is characterized by relatively high accumulation level of Cd,Cu,and Zn in its soils. | LI Xiaoyan CAO Yijin QI Le SHU Fu | 2012 | Chinese Journal Of Geochemistry2012，31，2： | 14 |
| 7 | Geologic characteristics of the Chang'E-3 exploration region显示文摘We present topographic,geomorphologic and compositional characteristics of a 1°×1°(~660 km2)region centered near the landing site of Chang’E-3 using the highest spatial resolution data available.We analyze the topography and slope using Digital Terrain Model(DTM)generated from Terrain Camera(TC)images.The exploration region is overall relatively flat and the elevation difference is less than 300 m,and the slopes of 80%area are less than 5°.Impact craters in the exploration region are classified into four types based on their degradation states.We investigate the wrinkle ridges visible in the exploration region in detail using TC and Lunar Reconnaissance Orbiter(LRO)Narrow Angle Camera(NAC)images.We calculate FeO and TiO2 abundances using Multispectral Imager(MI)data,and confirm two basaltic units:the northern part belongs to Imbrian low-Ti/very-low-Ti mare basalts,and the southern part is Eratosthenian low-Ti/high-Ti mare basalts.Finally,we produce a geological map and propose the geologic evolution of the exploration region.We also suggest several rover traverses to explore interesting targets and maximize the potential scientific output. | ZHAO JianNan HUANG Jun QIAO Le XIAO ZhiYong HUANG Qian WANG Jiang HE Qi XIAO Long | 2014 | Science China(Physics,Mechanics & Astronomy)2014，57，3： | 14 |
| 8 | Characterization of natural organic matter in water for optimizing water treatment and minimizing disinfection by-product formation显示文摘Introduction Natural organic matter(NOM)present in source water has significant impact on water treatment processes and on the quality of drinking water.NOM is a complex mixture of diverse groups of organic compounds,humic and fulvic acids,proteins,peptides,carbohydrates,and heterogeneous | Qi Zheng Xiaoqiu Yang Wenchao Deng X.Chris Le Xing-Fang Li | 2016 | Journal of Environmental Sciences2016，28，4： | 13 |
| 9 | Methylated and thiolated arsenic species for environmental and health research——A review on synthesis and characterization显示文摘Hundreds of millions of people around the world are exposed to elevated concentrations of inorganic and organic arsenic compounds, increasing the risk of a wide range of health effects. Studies of the environmental fate and human health effects of arsenic require authentic arsenic compounds. We summarize here the synthesis and characterization of more than a dozen methylated and thiolated arsenic compounds that are not commercially available. We discuss the methods of synthesis for the following14 trivalent(Ⅲ) and pentavalent() arsenic compounds: monomethylarsonous acid(MMA~Ⅲ), dicysteinylmethyldithioarsenite(MMA~Ⅲ(Cys)_2), monomethylarsonic acid(MMA~Ⅴ),monomethylmonothioarsonic acid(MMMTAⅤ) or monothio-MMA~Ⅴ, monomethyldithioarsonic acid(MMDTA~Ⅴ) or dithio-MMA~Ⅴ, monomethyltrithioarsonate(MMTTA~Ⅴ) or trithio-MMA~Ⅴ,dimethylarsinous acid(DMA~Ⅲ), dimethylarsino-glutathione(DMA~Ⅲ(SG)), dimethylarsinic acid(DMA~Ⅴ), dimethylmonothioarsinic acid(DMMTA~Ⅴ) or monothio-DMAⅤ, dimethyldithioarsinic acid(DMDTA~Ⅴ) or dithio-DMA~Ⅴ, trimethylarsine oxide(TMAO~Ⅴ), arsenobetaine(AsB), and an arsenicin-A model compound. We have reviewed and compared the available methods,synthesized the arsenic compounds in our laboratories, and provided characterization information. On the basis of reaction yield, ease of synthesis and purification of product, safety considerations, and our experience, we recommend a method for the synthesis of each of these arsenic compounds. | William R.Cullen Qingqing Liu Xiufen Lu Anthony McKnight-Whitford Hanyong Peng Aleksandra Popowich Xiaowen Yan Qi Zhang Michael Fricke Hongsui Sun X.Chris Le | 2016 | Journal of Environmental Sciences2016，28，11： | 12 |
| 10 | SbHKT1;4, a member of the high‐affinity potassium transporter gene family from Sorghum bicolor, functions to maintain optimal Na^+/K^+ balance under Na^+ stress显示文摘In halophytic plants, the high‐affinity potassium transporter HKT gene family can selectively uptake Kt in the presence of toxic concentrations of Nat. This has so far not been well examined in glycophytic crops. Here, we report the characterization of SbHKT1;4, a member of the HKT gene family from Sorghum bicolor. Upon Nat stress, SbHKT1;4 expression was more strongly upregulated in salt‐tolerant sorghum accession, correlating with a better balanced Nat/ Kt ratio and enhanced plant growth. Heterogeneous expression analyses in mutants of Saccharomyces cerevisiae and Arabidopsis thaliana indicated that overexpressing SbHKT1;4 resulted in hypersensitivity to Nat stress, and such hypersensitivity could be alleviated with the supply of elevated levels of Kt, implicating that SbHKT1;4 may mediate Kt uptake in the presence of excessive Nat. Further electrophysiological evidence demonstrated that SbHKT1;4 could transport Nat and Kt when expressed in Xenopus laevis oocytes. The relevance of the finding that SbHKT1;4 functions to maintain optimal Nat/Kt balance under Nat stress to the breeding of salt‐tolerant glycophytic crops is discussed. | Tian‐Tian Wang Zhi‐Jie Ren Zhi‐Quan Liu Xue Feng Rui‐Qi Guo Bao‐Guo Li Le‐Gong Li Hai‐Chun Jing | 2014 | Journal of Integrative Plant Biology2014，56，3： | 11 |
| 11 | Plant Genome Editing Using FnCpfl and LbCpf Nucleases at Redefined and Altered PAM-Sites显示文摘 | ZhaohuiZhong Yingxiao Zhang Qi You XU Tang Qiurong Ren Shishi Liu Lijia Yang Yan Wang Xiaopei Liu Binglin Liu Tao Zhang Xuelian Zheng Ysa Le Yong Zhang Yiping Qi | 2018 | Molecular Plant2018，11，7： | 10 |
| 12 | Regulation of Rice Tillering by RNA-Directed DNA Methylation at Miniature Inverted-Repeat Transposable Elements显示文摘Tillering is a major determinant of rice plant architecture and grain yield.Here,we report that depletion of rice OsNRPD1a and OsNRPD1b,two orthologs of the largest subunit of RNA polymerase IV,leads to a high-tillering phenotype,in addition to dwarfism and smaller panicles.OsNRPD1a and OsNRPD1b are required for the production of 24-nt small interfering RNAs that direct DNA methylation at transposable elements(TEs)including miniature inverted-repeat TEs(MITEs).Interestingly,many genes are regulated either positively or negatively by TE methylation.Among them,OsMIR156d and OsMIR156j,which promote rice tillering,are repressed by CHH methylation at two MITEs in the promoters.By contrast,D14,which suppresses rice tillering,is activated by CHH methylation at an MITE in its downstream.Our findings reveal regulation of rice tillering by RNA-directed DNA methylation at MITEs and provide potential targets for agronomic trait enhancement through epigenome editing. | Le Xu Kun Yuan Meng Yuan Xiangbing Meng Min Chen Jianguo Wu Jiayang Li Yijun Qi | 2020 | Molecular Plant2020，13，6： | 9 |
| 13 | Endothelial CDS2 deficiency causes VEGFA-mediated vascular regression and tumor inhibition显示文摘The response of endothelial cells to signaling stimulation is critical for vascular morphogenesis,homeostasis and function.Vascular endothelial growth factor-a(VEGFA)has been commonly recognized as a pro-angiogenic factor in vertebrate developmental,physiological and pathological conditions for decades.Here we report a novel finding that genetic ablation of CDP-diacylglycerol synthetase-2(CDS2),a metabolic enzyme that controls phosphoinositide recycling,switches the output of VEGFA signaling from promoting angiogenesis to unexpectedly inducing vessel regression.Live imaging analysis uncovered the presence of reverse migration of the angiogenic endothelium in cds2 mutant zebrafish upon VEGFA stimulation,and endothelium regression also occurred in postnatal retina and implanted tumor models in mice.In tumor models,CDS2 deficiency enhanced the level of tumorsecreted VEGFA,which in-turn trapped tumors into a VEGFA-induced vessel regression situation,leading to suppression of tumor growth.Mechanistically,VEGFA stimulation reduced phosphatidylinositol(4,5)-bisphosphate(PIP2)availability in the absence of CDS2-controlled-phosphoinositide metabolism,subsequently causing phosphatidylinositol(3,4,5)-triphosphate(PIP3)deficiency and F0X01 activation to trigger regression of CDS2-null endothelium.Thus,our data indicate that the effect of VEGFA on vasculature is context-dependent and can be converted from angiogenesis to vascular regression. | Wencao Zhao Le Cao Hanru Ying Wenjuan Zhang Dantong Li Xiaolong Ziiu Wenzhi Xue Shuang Wu Mengye Cao Cong Fu Haonan Qi Yimei Hao Yun-Chi Tang Jun Qin Tao PZhong Xiaoxi Lin Luyang Yu Xuri Li Lin Li Dianqing Wu Weijun Pan | 2019 | Cell Research2019，29，11： | 9 |
| 14 | Metrnl deficiency decreases blood HDL cholesterol and increases blood triglyceride显示文摘Dyslipidemia is a risk factor for cardiovascular diseases and type 2 diabetes.Several adipokines play important roles in modulation of blood lipids.Metrnl is a recently identified adipokine,and adipose Metrnl participates in regulation of blood triglyceride(TG).In this study,we generated Metrnl global,intestine-specific and liver-specific knockout mice,and explored the effects of Metrnl on serum lipid parameters.Global knockout of Metrnl had no effects on serum lipid parameters under normal chow diet,but increased blood TG by 14%,and decreased total cholesterol(TC)by 16%and high density lipoprotein cholesterol(HDL-C)by 24%under high fat diet.Nevertheless,intestine-specific knockout of Metrnl did not alter the serum lipids parameters under normal chow diet or high fat diet.Notably,liver-specific knockout of Metrnl decreased HDL-C by 24%,TC by 20%and low density lipoprotein cholesterol(LDL-C)by 16%without alterations of blood TG and nonesterified fatty acids(NEFA)under high fat diet.But deficiency of Metrnl in liver did not change VLDL secretion and expression of lipid synthetic and metabolic genes.We conclude that tissue-specific Metrnl controls different components of blood lipids.In addition to modulation of blood TG by adipose Metrnl,blood HDL-C is regulated by liver Metrnl. | Qi Qi Wen-jun Hu Si-li Zheng Sai-long Zhang Ying-ying Le Zhi-yong Li Chao-yu Miao | 2020 | Acta Pharmacologica Sinica2020，41，12： | 9 |
| 15 | An expression atlas of miRNAs in Arabidopsis thaliana显示文摘MicroRNAs(miRNAs) are small non-coding RNAs that regulate a variety of biological processes. miRNA expression often exhibits spatial and temporal specificity. However, genome-wide miRNA expression patterns in different organs during development of Arabidopsis thaliana have not yet been systemically investigated. In this study, we sequenced small RNA libraries generated from 27 different organ/tissue types, which cover the entire life cycle of Arabidopsis. Analysis of the sequencing data revealed that most miRNAs are ubiquitously expressed, whereas a small set of miRNAs display highly specific expression patterns. In addition, different miRNA members within the same family have distinct spatial and temporal expression patterns. Moreover, we found that some miRNAs are produced from different arms of their hairpin precursors at different developmental stages. This work provides new insights into the regulation of miRNA biogenesis and a rich resource for future investigation of miRNA functions in Arabidopsis. | Le Xu Yugang Hu Ying Cao Jingrui Li Ligeng Ma Yan Li Yijun Qi | 2018 | Science China(Life Sciences)2018，61，2： | 8 |
| 16 | An individualized immune signature of pretreatment biopsies predicts pathological complete response to neoadjuvant chemoradiotherapy and outcomes in patients with esophageal squamous cell carcinoma显示文摘No clinically available biomarkers can predict pathological complete response(pCR)for esophageal squamous cell carcinomas(ESCCs)with neoadjuvant chemoradiotherapy(nCRT).Considering that antitumor immunity status is an important determinant for nCRT,we performed an integrative analysis of immune-related gene profiles from pretreatment biopsies and constructed the first individualized immune signature for pCR and outcome prediction of ESCCs through a multicenter analysis.During the discovery phase,14 differentially expressed immune-related genes(DEIGs)with greater than a twofold change between pCRs and less than pCRs( | Chaoqi Zhang Guochao Zhang Nan Sun Zhen Zhang Liyan Xue Zhihui Zhang Haijun Yang Yuejun Luo Xiaoli Zheng Yonglei Zhang Yufen Yuan Ruixue Lei Zhaoyang Yang Bo Zheng Le Wang Yun Che Feng Wang Sihui Wang Shugeng Gao Qi Xue Yi Zhang Jie He | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 7 |
| 17 | A role for the RNA-binding protein MOS2 in microRNA maturation in Arabidopsis显示文摘microRNAs (miRNAs ) 玩在基因表示的规定的重要角色。在 Arabidopsis,成熟 miRNAs 被形成用色子赌博的身体(D 身体) 的原子 HYL1/SE/DCL1 建筑群从主要 miRNA 抄本(pri-miRNAs ) 处理。这里,我们报导 RNA 有约束力的蛋白质 MOS2 绑在 pri-miRNAs 并且涉及 pri-miRNAs 的有效处理。MOS2 不与 HYL1, SE,和 DCL1 交往并且不在 D 身体是局部性的。当 MOS2 不在时,有趣地,由 HYL1 的 pri-miRNAs 的招募极大地被减少,在 D 身体的 HYL1 的本地化被损害。这些数据建议 MOS2 支持通过由用色子赌博的建筑群便于 pri-miRNAs 的招募处理的 pri-miRNA。 | Xueying Wu Yupeng Shi Jingrui Li Le Xu Yuda Fang Xin Li Yijun Qi | 2013 | Cell Research2013，23，5： | 7 |
| 18 | A Resource for Inactivation of MicroRNAs Using Short Tandem Target Mimic Technology in Model and Crop Plants显示文摘microRNAs (miRNAs)are endogenous small non-coding RNAs that bind to mRNAs and target them for cleavage and/or translational repression,leading to gene silencing.We previously developed short tandem target mimic (STTM)technology to deactivate endogenous miRNAs in Arabidopsis.Here,we created hundreds of STTMs that target both conserved and species-specific miRNAs in Arabidopsis,tomato,rice,and maize,providing a resource for the functional interrogation of miRNAs.We not only revealed the functions of several miRNAs in plant development,but also demonstrated that tissue-specific inactivation of a few miRNAs in rice leads to an increase in grain size without adversely affecting overall plant growth and development.RNA-seq and small RNAseq analyses of STTM156/157 and STTM165/166 transgenic plants revealed the roles of these miRNAs in plant hormone biosynthesis and activation,secondary metabolism,and ion-channel activity-associated electrophysiology,demonstrating that STTM technology is an effective approach for studying miRNA functions.To facilitate the study and application of STTM transgenic plants and to provide a useful platform for storing and sharing of information about miRNA-regulated gene networks,we have established an online Genome Browser (http://gffzzc750bc0108fa4044sfpncnx5c0wfk6c5v.ffgz.tsg.suse.edu.cn/designindex2.php) to display the transcriptomic and miRNAomic changes in STTMinduced miRNA knockdown plants. | Ting Peng Mengmeng Qiao Haiping Liu Sachin Teotia Zhanhui Zhang Yafan Zhao Bobo Wang Dongjie Zhao Lina Shi Cui Zhang Brandon Le Kestrel Rogers Chathura Gunasekara Haitang Duan Yiyou Gu Lei Tian Jinfu Nie Jian Qi Fanrong Meng Lan Huang Qinghui Chen Zhenlin Wang Jinshan Tang Xiaoqing Tang Ting Lan Xuemei Chen Hairong Wei Quanzhi Zhao Guiliang Tang | 2018 | Molecular Plant2018，11，11： | 7 |
| 19 | Surgical outcome and patient satisfaction after Z-epicanthoplasty and blepharoplasty显示文摘AIM: To evaluate surgical outcomes of modified Z-epicanthoplasty with blepharoplasty that we previously reported from the patient's perspective using patient-reported outcome measures(PROMs) and patient satisfaction scores.METHODS: A total of patients(n=180) who underwent the surgery between January 2013 and June 2016 were randomly selected. Standardized patient satisfaction forms(total score, 40) and validated PROMs questionnaires(total score, 12) were sent to patients for completion. PROMs assesses the severity of scarring, pain and asymmetry, as well as functional and appearance issues.RESULTS: All patients were female, ranging from 18 to 35 years old(mean=24). The response rate was 73.3%(n=132). The majority of patients reported good or excellent outcomes based on PROM analysis. Patients reported minimum or non-visible scarring at both the double eyelid surgical scar(85.6%) and the inner canthus(80.3%). Issues concerning function and appearance were minimal as 80.3% reported satisfaction with both domains. Notably, the majority of patients reported either a high or very high satisfaction rate to yield a mean score of 104 out of 120(P<0.05).CONCLUSION: Integration of our modified Z-epicanthoplasty with blepharoplasty produces good outcomes based on PROM results, which shows a positive linear relationship with patient satisfaction scores. | Jing-Yi Zhao Xiao-Shuang Guo Guo-Dong Song Xian-Lei Zong Xiao-Nan Yang Le Du Chen-Zhi Lai Zuo-Liang Qi Xiao-Lei Jin | 2018 | International Journal of Ophthalmology(English edition)2018，11，12： | 7 |
| 20 | Systematic identification of endogenous RNA polymeraseⅢpromoters for efficient RNA guidebased genome editing technologies in maize显示文摘Single-guide RNA(sg RNA) is one of the two core components of the CRISPR(clustered regularly interspaced short palindromic repeat)/Cas(CRISPR-associated) genome-editing technology. We established an in vitro Traffic Light Reporter(TLR) system, which is designated as the same colors as traffic lights such as green, red and yellow were produced in cells. The TLR can be readily used in maize mesophyll protoplast for a quick test of promoter activity. The TLR assay indicates the variation in transcription activities of the seven Pol III promoters, from 3.4%(U6-1) to over 21.0%(U6-6). The U6-2 promoter, which was constructed to drive sg RNA expression targeting the Zm Wx1 gene, yielded mutation efficiencies ranging from 48.5% to 97.1%. Based on the reported and unpublished data, the in vitro TLR assay results were confirmed to be a readily system and may be extended to other plant species amenable to efficient genome editing via CRISPR/Cas. Our efforts provide an efficient method of identifying native Pol III-recognized promoters for RNA guide-based genome-editing systems in maize. | Xiantao Qi Le Dong Changlin Liu Long Mao Fang Liu Xin Zhang Beijiu Cheng Chuanxiao Xie | 2018 | The Crop Journal2018，6，3： | 7 |
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