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| 1 | Temperature variations recovered from tree-rings in the middle Qilian Mountain over the last millennium显示文摘Based on the cross-dated tree-ring samples collected from the middle Qilian Moun- tain, a standard ring-width chronology had been developed, which covered the period AD 1000 to 2000. The correlations between the chronology and climatic records from the nearby meteorological stations indicated that temperature was the dominant climatic factor for tree growth at upper timberline, and the most important climatic factor for the tree growth in the area was the mean temperature from previous December to current April. The temperature variations recovered from the ring-width data showed a cold period during the “Little Ice Age” and the con- tinuous warming during the twentieth century. Comparison between the ring-width chronology and δ18O records from the Dunde ice core in the Qilian Mountain indicated that there was a con- sistent trend in both time series. A significant correlation existed between our ring-width chro- nology and the Northern Hemispheric temperature, suggesting that the climate changes in the Qilian Mountain were not only driven by regional factors, but also responsive to the global cli- mate. | LIU Xiaohong1,2, QIN Dahe1, SHAO Xuemei , CHEN Tuo1 & REN Jiawen12 1. Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000, China 2. Institute of Geographical Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China | 2005 | Science China Earth Sciences2005，48，4： | 66 |
| 2 | Trends of incidence rate and age at diagnosis for cervical cancer in China,from 2000 to 2014显示文摘Objective: To analyze the trends of incidence rate and age at diagnosis for cervical cancer incidence in China using population-based cancer registration data from 2000 to 2014.Methods: Data were from National Central Cancer Registry of China.Crude incidence rates(CIRs),age-specific incidence rate,age-standardized incidence rates(ASIRs),age percentage distribution,standardized age percentage distribution,mean age at diagnosis and standardized mean age at diagnosis for cervical cancer in all areas of China,urban China and rural China were calculated separately.The world Segi's population was applied to remove the age structure influence.Joinpoint regression was performed to obtain average annual percent change(AAPC) and ageperiod-cohort analysis was used to examine the incidence trends.Results: CIRs and ASIRs for cervical cancer increased in China from 2000 to 2014.The AAPC of ASIRs in China was at 9.2% [95% confidence interval(95% CI): 7.0%–11.5%,P<0.05],and the AAPC in rural areas was relatively high.The age-specific incidence rate in groups aged 0–69 years have significantly increased over time.Groups aged 40–69 years showed the highest incidence risk,and the annual percent changes(APCs) of incidence rate in groups aged 40–59 years in urban China and groups aged 0–49 years in rural China were more than 10%.For each age group,the urban-to-rural incidence rate ratios(IRRs) got close to 1 over time.There were clear birth cohort effects in successive generations born from 1940 to 1970 in China.In rural China,the standardized mean age at diagnosis had significantly declined by 5.18 years.In China,the main peak and secondary peak of standardized age percentages appeared in the groups aged 45–49 and 40–44 years,respectively.In rural China,the main peak of standardized age percentage moved from the group aged 55–59 years to the group aged 45–49 years,and the standardized age percentages of groups aged 25–34 years also increased.In China,the standardized age percentages has significantly increased in groups aged 35–64 and 30–64 years over time,and accounted for about80% and 85% in 2014,respectively.Conclusions: The cervical cancer incidence increased in China and the gap of incidence between urban and rural China was narrowed.The trends of increasing cervical cancer incidence among younger women existed in China,especially in rural China.A more appropriate screening,vaccination and health education strategies should be established. | Xueting Li Rongshou Zheng Xuemei Li Haibin Shah Qi Wu Yan Wang Wanqing Chen | 2017 | Chinese Journal of Cancer Research2017，29，6： | 64 |
| 3 | Effects of dietary Clostridium butyricum supplementation on growth performance,intestinal development,and immune response of weaned piglets challenged with lipopolysaccharide显示文摘Background: Weanling pigs, with immature immune system and physiological function, usually experience postweaning diarrhea. This study determined the effects of dietary Clostridium butyricum supplementation on growth performance, diarrhea, and immunity of weaned pigs challenged with lipopolysaccharide(LPS).Methods: In Experiment(Exp.) 1,144 weaned piglets were weaned at 21 d and randomly assigned to six groups,with six replicates per group and four pigs per replicate, receiving a control diet(CON) or diet supplemented with antibiotics(AB) or C. butyricum(CB)(0.1%, 0.2%, 0.4%, or 0.8%), respectively. All diets in Exp. 1 were a highly digestible basal diet, with 3,000 mg/kg zinc oxide supplied in the first 2 wk only. In Exp. 2, 180 piglets were weaned at 21 d and randomly assigned to five groups, with six replicates per group and six pigs per replicate, receiving CON, AB, or CB(0.2%, 0.4%, or 0.6%) diets. The digestibility of diets was lower than those in Exp. 1, and did not include zinc oxide. At 36 d of Exp. 2, 12 piglets were selected from each of the CON and 0.4% CB groups, six piglets were intraperitoneally injected with LPS(50 μg/kg body weight) and the other six piglets with normal saline;animals were killed at 4 h after injection to collect blood, intestine, and digesta samples for biochemical analysis.Results: In Exp. 1, CB and AB diets had no effect on growth performance of piglets. In Exp. 2, 0.4% CB decreased feed-gain ratio(P < 0.1), diarrhea score(P < 0.05), and increased duodenal, jejunal, and ileal villus height and jejunal villus height/crypt depth(P < 0.05). The 0.4% CB decreased the plasma tumor necrosis factor(TNF) α(P < 0.05) but increased ileal mucosa IL-10 and TLR2 mRNA expression(P < 0.05). Furthermore, 0.4% CB altered the microbial profile, with Bacillus and Ruminococcaceae UGG-003 at genus level and Lactobacillus casei and Parasutterella secunda at species level were higher than CON in colonic content(P < 0.05).Conclusions: Dietary C. butyricum supplementation had positive effects on growth of weaned piglets with less digestible diets. There was a tendency to reduce the feed-gain ratio, which could reduce feed costs in pig production. Moreover, C. butyricum decreased post-weaning diarrhea by improving the intestinal morphology,intestinal microflora profile, and immune function. | Ling Chen Shuang Li Jie Zheng Wentao Li Xuemei Jiang Xilun Zhao Jian Li Lianqiang Che Yan Lin Shengyu Xu Bin Feng Zhengfeng Fang De Wu | 2018 | Journal of Animal Science and Biotechnology2018，9，4： | 26 |
| 4 | Meta-analyses on prevalence of selected Parkinson’s nonmotor symptoms before and after diagnosis显示文摘Background:Nonmotor symptoms are common among patients with Parkinson’s disease(PD)and some may precede disease diagnosis.Methods:We conducted a meta-analysis on the prevalence of selected nonmotor symptoms before and after PD diagnosis,using random-effect models.We searched PubMed(1965 through October/November 2012)for the following symptoms:hyposmia,constipation,rapid eye movement sleep behavior disorder,excessive daytime sleepiness,depression,and anxiety.Eligible studies were publications in English with original data on one or more of these symptoms.Results:The search generated 2,373 non-duplicated publications and 332 met the inclusion criteria,mostly(n=320)on symptoms after PD diagnosis.For all symptoms,the prevalence was substantially higher in PD cases than in controls,each affecting over a third of the patients.Hyposmia was the most prevalent(75.5%in cases vs.19.1%in controls),followed by constipation(50%vs.17.7%),anxiety(39.9%vs.19.1%),rapid eye movement sleep behavior disorder(37.0%vs.7.0%),depression(36.6%vs.14.9%),and excessive daytime sleepiness(33.9%vs.10.5%).We observed substantial heterogeneities across studies and meta-regression analyses suggested that several factors might have contributed to this.However,the prevalence estimates were fairly robust in several sensitivity analyses.Only 20 studies had data on any symptoms prior to PD diagnosis,but still the analyses revealed higher prevalence in future PD cases than in controls.Conclusion:These symptoms are common among PD patients both before and after diagnosis.Further studies are needed to understand the natural history of nonmotor symptoms in PD and their etiological and clinical implications. | Honglei Chen Edward J Zhao Wen Zhang Yi Lu Rui Liu Xuemei Huang Anna J Ciesielski-Jones Michele A Justice Deborah S Cousins Shyamal Peddada | 2015 | Translational Neurodegeneration2015，4，1： | 18 |
| 5 | Buyang Huanwu Decoction regulates neural stem cell behavior in ischemic brain显示文摘The traditional Chinese medicine Buyang Huanwu Decoction has been shown to improve the neurological function of patients with stroke.However,the precise mechanisms underlying its effect remain poorly understood.In this study,we established a rat model of cerebral ischemia by middle cerebral artery occlusion and intragastrically administered 5 g/kg Buyang Huanwu Decoction,once per day,for 1,7,14 and 28 days after cerebral ischemia.Immunohistochemical staining revealed a number of cells positive for the neural stem cell marker nestin in the cerebral cortex,the subventricular zone and the ipsilateral hippocampal dentate gyrus in rat models of cerebra ischemia.Buyang Huanwu Decoction significantly increased the number of cells positive for5-bromodeoxyuridine(BrdU),a cell proliferation-related marker,microtubule-associated protein-2,a marker of neuronal differentiation,and growth-associated protein 43,a marker of synaptic plasticity in the ischemic rat cerebral regions.The number of positive cells peaked at 14 and 28 days after intragastric administration of Buyang Huanwu Decoction.These findings suggest that Buyang Huanwu Decoction can promote the proliferation and differentiation of neural stem cells and enhance synaptic plasticity in ischemic rat brain tissue. | Baiyan Liu Guangxian Cai Jian Yi Xuemei Chen | 2013 | Neural Regeneration Research2013，8，25： | 17 |
| 6 | Particle number concentration, size distribution and chemical composition during haze and photochemical smog episodes in Shanghai显示文摘The aerosol number concentration and size distribution as well as size-resolved particle chemical composition were measured during haze and photochemical smog episodes in Shanghai in 2009.The number of haze days accounted for 43%,of which 30%was severe(visibility<2 km)and moderate(2 km≤visibility<3 km)haze,mainly distributed in winter and spring.The mean particle number concentration was about 17,000/cm3in haze,more than 2 times that in clean days.The greatest increase of particle number concentration was in 0.5–1μm and 1–10μm size fractions during haze events,about 17.78times and 8.78 times those of clean days.The largest increase of particle number concentration was within 50–100 nm and 100–200 nm fractions during photochemical smog episodes,about 5.89 times and 4.29 times those of clean days.The particle volume concentration and surface concentration in haze,photochemical smog and clean days were102,49,15μm3/cm3and 949,649,206μm2/cm3,respectively.As haze events got more severe,the number concentration of particles smaller than 50 nm decreased,but the particles of 50–200 nm and 0.5–1μm increased.The diurnal variation of particle number concentration showed a bimodal pattern in haze days.All soluble ions were increased during haze events,of which NH4+,SO42-and NO3-increased greatly,followed by Na+,K+,Ca2+and Cl-.These ions were very different in size-resolved particles during haze and photochemical smog episodes. | Xuemei Wang Jianmin Chen Tiantao Cheng Renyi Zhang Xinming Wang | 2014 | Journal of Environmental Sciences2014，26，9： | 17 |
| 7 | Sequence Thickness and its Response to Episodic Tectonic Evolution in Paleogene Qikou Sag, Bohaiwan Basin显示文摘The sequence stratigraphy framework of Paleogene of Qikou Sag has been built in this paper. The evolution law of episodic subsidence in Qikou Sag has been expounded, with the analysis of the characteristic of sequence thickness, as well as the study of consedimental tectonic activity, subsidence history, paleogeomorphology and the coupling relationship among them. The faults controlling action was the dominant mechanism during rifting stage I , as the depression was the dominant mechanism during rifting stage III, which embodies a process from intense disparity fault depression to integral subsidence. Meanwhile, under the control of regional faulting and later stage depression, subsidence rate centers and paleogeomorphic concave centers are always corresponding to the sequence thickness centers, and the changes of subsidence centers and paleogeomorphology concave centers are consistent with the migration of thickness centers, which presents the restriction, coupling and response among them and clarifies the law that tectonic activity controls the basin fill by means of controlling the subsidence rate and paleogeomorphology from contributing factor angle. On this basis, the dynamic evolution of the composite pattern of structure-subsidence-deposition in the fault controlling actic region during the three rifting stages and their petroleum geological significance has been discussed. | CHEN Si WANG Hua ZHOU Lihong HUANG Chuanyan WANG Jiahao REN Peigang XIANG Xuemei | 2012 | Acta Geologica Sinica(English Edition)2012，86，5： | 11 |
| 8 | Long-term trends of fine particulate matter and chemical composition in the Pearl River Delta Economic Zone (PRDEZ), China显示文摘在下午 2.5 层次理解趋势为提出是必要的干净空气计划。这份报纸在珀尔河三角洲经济地区(PRDEZ ) 为 2000 年从各种各样的出版来源分析下午 2.5 数据到 2010。在下午 2.5 团集中的长期的变化被分析。结果给那位首相看 2.5, 器官的碳(OC ) ,元素的碳(EC ) ,并且那么 42 显示出一个类似的趋势,在 2005 前增加然后稍微减少。年度普通下午 2.5 集中从 49.1 g ? | Xuemei WANG Weihua CHEN Duohong CHEN Zhiyong WU Qi Fan | 2016 | Frontiers of Environmental Science & Engineering2016，10，1： | 11 |
| 9 | Recently duplicated sesterterpene(C25) gene clusters in Arabidopsis thaliana modulate root microbiota显示文摘Land plants co-speciate with a diversity of continually expanding plant specialized metabolites(PSMs) and root microbial communities(microbiota).Homeostatic interactions between plants and root microbiota are essential for plant survival in natural environments.A growing appreciation of microbiota for plant health is fuelling rapid advances in genetic mechanisms of controlling microbiota by host plants.PSMs have long been proposed to mediate plant and single microbe interactions.However,the effects of PSMs,especially those evolutionarily new PSMs,on root microbiota at community level remain to be elucidated.Here,we discovered sesterterpenes in Arabidopsis thaliana,produced by recently duplicated prenyltransferase-terpene synthase(PT-TPS) gene clusters,with neo-functionalization.A single-residue substitution played a critical role in the acquisition of sesterterpene synthase(sesterTPS) activity in Brassicaceae plants.Moreover,we found that the absence of two root-specific sesterterpenoids,with similar chemical structure,significantly affected root microbiota assembly in similar patterns.Our results not only demonstrate the sensitivity of plant microbiota to PSMs but also establish a complete framework of host plants to control root microbiota composition through evolutionarily dynamic PSMs. | Qingwen Chen Ting Jiang Yong-Xin Liu Haili Liu Tao Zhao Zhixi Liu Xiangchao Gan Asis Hallab Xuemei Wang Juan He Yihua Ma Fengxia Zhang Tao Jin M. Eric Schranz Yong Wang Yang Bai Guodong Wang | 2019 | Science China(Life Sciences)2019，62，7： | 11 |
| 10 | Regulation of small RNA stability: methylation and beyond显示文摘作为 RNA silencing 的中央部件,小 RNA 在优核质在许多生物过程起多样、重要的作用。在小 RNA 的层次的异常减小或举起与许多发展、生理的缺点被联系。在里面小 RNA 的 vivo 层次精确通过 modulating 被调整他们的生物的续生说和周转的率。2 ′ 3 ′ 上的 -O-methylation;终端核糖是增加小 RNA 的稳定性的主要机制。在植物,在动物的交往 Piwi RNA (piRNAs ) ,和在果蝇的 siRNAs 的小 RNA methyltransferase HUA ENHANCER1 (HEN1 ) 和它的相当或相同的事物甲醇化物 microRNAs 和小介入 RNA (siRNAs ) 。3 ′核苷酸增加,特别 uridylation,和 3 ′ -5′exonucleolytic 降级是主要机制那周转小 RNA。影响小 RNA 稳定性的另外的机制包括互补 RNA,在小 RNA 序列的 cis 元素和 RNA 有约束力的蛋白质。调查是进行中的进一步理解小 RNA 稳定性怎么在 vivo 影响他们的累积以便在生物工学和治疗学的应用改进 RNA silencing 的利用。 | Lijuan Ji Xuemei Chen | 2012 | Cell Research2012，22，4： | 10 |
| 11 | Climatic significance of the stable carbon isotope composition of tree-ring cellulose:Comparison of Chinese hemlock (Tsuga chinensis Pritz) and alpine pine (Pinus densata Mast) in a temperate-moist region of China显示文摘In the reconstruction of past climate using stable carbon isotope composition (δ13C) in tree ring,the responses of the stable carbon composition (δ13C) of multiple tree species to environmental factors must be known detailedly. This study presented two δ13C series in annual tree rings for Chinese hem-lock (Tsuga chinensis Pritz) and alpine pine (Pinus densata Mast),and investigated the relationships between climatic parameters and stable carbon discrimination (△13C) series,and evaluated the poten-tial of climatic reconstruction using △13C in both species,in a temperate-moist region of Chuanxi Pla-teau,China. The raw δ13C series of the two species was inconsistent,which may be a result of different responses caused by tree's inherent physiological differences. After removing the low-frequency ef-fects of CO2 concentration,the high-frequency (year-to-year) inter-series correlation of △13C was strong,indicating that △13C of the two tree species were controlled by common environmental conditions. The △13C series of the species were most significantly correlated with temperature and moisture stress,but in different periods and intensity between the species. During the physiological year,the impacts of temperature and moisture stress on △13C occur earlier for Chinese hemlock (previous December to February for moisture stress and February to April for temperature,respectively) than for alpine pine (March to May for moisture stress and April to July for temperature,respectively). In addition,in temperate-moist regions,the control on △13C of single climatic parameter was not strongly dominant and the op-timal multiple regressions functions just explained the 38.5% variance of the total. Therefore,there is limited potential for using δ13C alone to identify clear,reliable climatic signals from two species. | LIU XiaoHong SHAO XueMei WANG LiLi ZHAO LiangJu WU Pu CHEN Tuo QIN DaHe REN JiaWen | 2007 | Science China Earth Sciences2007，50，7： | 10 |
| 12 | Finite-Time Neural Funnel Control for Motor Servo Systems with Unknown Input Constraint显示文摘In this paper, a finite-time neural funnel control(FTNFC) scheme is proposed for motor servo systems with unknown input constraint. To deal with the non-smooth input saturation constraint problem, a smooth non-affine function of the control input signal is employed to approximate the saturation constraint, which is further transformed into an affine form according to the mean-value theorem. A fast terminal sliding mode manifold is constructed by using a novel funnel error variable to force the tracking error falling into a prescribe boundary within a finite time. Then, a simple sigmoid neural network is utilized to approximate the unknown system nonlinearity including the saturation.Different from the prescribed performance control(PPC), the proposed finite-time neural funnel control avoids using the inverse transformed function in the controller design, and could guarantee the prescribed tracking performance without knowing the saturation bounds in prior. The effectiveness and superior performance of the proposed method are verified by comparative simulation results. | CHEN Qiang TANG Xiaoqing NAN Yurong REN Xuemei | 2017 | Journal of Systems Science & Complexity2017，30，3： | 9 |
| 13 | CAS9 is a genome mutator by directly disrupting DNA-PK dependent DNA repair pathway显示文摘With its high efficiency for site-specific genome editing and easy manipulation,the clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR associated protein 9(CAS9)system has become the most widely used gene editing technology in biomedical research.In addition,significant progress has been made for the clinical development of CRISPR/CAS9 based gene therapies of human diseases,several of which are entering clinical trials.Here we report that CAS9 protein can function as a genome mutator independent of any exogenous guide RNA(gRNA)in human cells,promoting genomic DNA double-stranded break(DSB)damage and genomic instability.CAS9 interacts with the KU86 subunit of the DNA-dependent protein kinase(DNA-PK)complex and disrupts the interaction between KU86 and its kinase subunit,leading to defective DNA-PK-dependent repair of DNA DSB damage via non-homologous end-joining(NHEJ)pathway.XCAS9 is a CAS9 variant with potentially higher fidelity and broader compatibility,and dCAS9 is a CAS9 variant without nuclease activity.We show that XCAS9 and dCAS9 also interact with KU86 and disrupt DNA DSB repair.Considering the critical roles of DNA-PK in maintaining genomic stability and the pleiotropic impact of DNA DSB damage responses on cellular proliferation and survival,our findings caution the interpretation of data involving CRISPR/CAS9-based gene editing and raise serious safety concerns of CRISPR/CAS9 system in clinical application. | Shuxiang Xu Jinchul Kim Qingshuang Tang Qu Chen Jingfeng Liu Yang Xu Xuemei Fu | 2020 | Protein & Cell2020，11，5： | 8 |
| 14 | Climatic significance of stable carbon isotope in tree rings of Abies spectabibis in southeastern Tibet显示文摘The annually cross-dated stable carbon isotope of tree-ring a-cellulose of Abies spectabibis collected from the southeastern Tibetan Plateau is used to examine its rela-tionship with climatic parameters. The residual D13C series in treerings is constructed after removing the effects of age trend and rising CO2. We found a close relationship between D13C in tree rings and the relative humidity of September—November of the previous year measured at the nearby Ny-ingchi Meteorological Station, albeit a strong 搇agged effect? Thus we developed a transfer function to reconstruct the autumn relative humidity for the Nyingchi region, which explained 37.9% of the total variance (p < 0.001). Our results suggest a high frequency and moderate amplitude variance of the relative humidity before 1800, and the variance re-versed afterwards. | LIU Xiaohong Qin Dahe SHAO Xuemei CHEN Tuo REN Jiawen | 2003 | Chinese Science Bulletin2003，48，18： | 8 |
| 15 | An observation-based perspective of winter haze days in four major polluted regions of China显示文摘An observation-based approach is used to examine key characteristics of winter haze days in four major polluted regions in China.Major findings in this study are:first,there was no significant trend in the number of winter haze days in most provinces and districts in eastern China from 1973 to 2012,contrary to the 2.5-fold increase in the emissions of particulate matter and its precursors(PM emissions)in the same period of time.Second,meteorological and climate conditions rather than PM emissions are in control of the interannual variabilities and trends of winter haze days.These interannual variabilities(ranging from 24 to 125%)pose a substantial masking effect that must be overcome by any control of PM emissions before its impact becomes statistically detectable.Finally,we find that global warming may have contributed significantly to the trend of winter haze days in eastern China. | Lu Mao Run Liu Wenhui Liao Xuemei Wang Min Shao Shaw Chen Liu Yuanhang Zhang | 2019 | National Science Review2019，6，3： | 7 |
| 16 | A Resource for Inactivation of MicroRNAs Using Short Tandem Target Mimic Technology in Model and Crop Plants显示文摘microRNAs (miRNAs)are endogenous small non-coding RNAs that bind to mRNAs and target them for cleavage and/or translational repression,leading to gene silencing.We previously developed short tandem target mimic (STTM)technology to deactivate endogenous miRNAs in Arabidopsis.Here,we created hundreds of STTMs that target both conserved and species-specific miRNAs in Arabidopsis,tomato,rice,and maize,providing a resource for the functional interrogation of miRNAs.We not only revealed the functions of several miRNAs in plant development,but also demonstrated that tissue-specific inactivation of a few miRNAs in rice leads to an increase in grain size without adversely affecting overall plant growth and development.RNA-seq and small RNAseq analyses of STTM156/157 and STTM165/166 transgenic plants revealed the roles of these miRNAs in plant hormone biosynthesis and activation,secondary metabolism,and ion-channel activity-associated electrophysiology,demonstrating that STTM technology is an effective approach for studying miRNA functions.To facilitate the study and application of STTM transgenic plants and to provide a useful platform for storing and sharing of information about miRNA-regulated gene networks,we have established an online Genome Browser (http://gffzzc750bc0108fa4044s5pf05qn65nuw6cnf.ffgz.tsg.suse.edu.cn/designindex2.php) to display the transcriptomic and miRNAomic changes in STTMinduced miRNA knockdown plants. | Ting Peng Mengmeng Qiao Haiping Liu Sachin Teotia Zhanhui Zhang Yafan Zhao Bobo Wang Dongjie Zhao Lina Shi Cui Zhang Brandon Le Kestrel Rogers Chathura Gunasekara Haitang Duan Yiyou Gu Lei Tian Jinfu Nie Jian Qi Fanrong Meng Lan Huang Qinghui Chen Zhenlin Wang Jinshan Tang Xiaoqing Tang Ting Lan Xuemei Chen Hairong Wei Quanzhi Zhao Guiliang Tang | 2018 | Molecular Plant2018，11，11： | 7 |
| 17 | Traditional Chinese Medicine syndrome-related herbal prescriptions in treatment of malignant tumors显示文摘OBJECTIVE: To investigate the distribution characteristics of TCM syndromes and the related herbal prescriptions for malignant tumors (MT). METHODS: A clinical database of the TCM syndromes and the herbal prescriptions in treatment of 136 MT patients were established. The data were then analyzed using cluster and frequency analysis. RESULTS: According to the cluster analysis, the TCM syndromes in MT patients mainly included two patterns: deficiency of both Qi and Yin and internal accumulation of toxic heat. The commonly-prescribed herbs were Huangqi (Astraglus), Nüzhen-zi (Fructus Ligustri Lucidi), Lingzhi (Ganoderma Lucidum), Huaishan (Dioscorea Opposita), Xiakucao (Prunella Vulgaris), and Baihuasheshecao (Herba Hedyotidis). CONCLUSION: Deficiency of Qi and Yin is the primary syndrome of MT, and internal accumulation of toxic heat is the secondary syndrome. The herbs for Qi supplementation and Yin nourishment are mainly used, with the assistance of herbs for heat-clearance and detoxification. | Zhizhen Liu Songyi Chen Jing Cai Enda Zhang Lan Lan Junting Zheng Lianming Liao Xuemei Yang Changen Zhou Jian Du | 2013 | Journal of Traditional Chinese Medicine2013，33，1： | 7 |
| 18 | Host-guest supramolecular chemistry at solid-liquid interface:An important strategy for preparing two-dimensional functional nanostructures显示文摘Supramolecular self-assembly,an important strategy in nanotechnology,has been widely studied in the past two decades.In this review,we have introduced the recent progress on construction of two-dimensional(2D)nanostructures by host-guest supramolecular chemistry at solid-liquid interface,and the interactions between the host assembly and the guest molecules are the major concerns.At first,the hydrogen bonds connected hybrid structures are discussed.And then we have paid a close attention on the surface-confined condensation reactions that has flourished recently in direct preparing novel nanostructures with increasing structural complexity.In the end,the cavity confinement of the 2D supramolecular host-guest architectures has been studied.On the basis of the above-mentioned interactions,a group of functional hybrid structures have been prepared.Notably,scanning tunneling microscopy(STM),a unique technique to probe the surface morphology and information at the single molecule level,has been used to probe the formed structures on highly oriented pyrolytic graphite(HOPG)surface. | ZHANG XueMei ZENG QingDao WANG Chen | 2014 | Science China Chemistry2014，57，1： | 6 |
| 19 | Antigenically dominant proteins within the human liver mitochondrial proteome identified by monoclonal antibodies显示文摘Analysis of the mitochondrial proteome would provide valuable insight into the function of this important organelle, which plays key roles in energy metabolism, apoptosis, free radical production, thermogenesis, and calcium signaling. It could also increase our understanding about the mechanisms that promote mitochondrial disease. To identify proteins that are antigenically dominant in human liver mitochondria, we generated >240 hybridoma cell lines from native mitochondrial proteins after cell fusion, screening, and cloning. Antibodies that recognized mitochondrial proteins were identified by screening human liver cDNA expression libraries. In this study, we identified 6 major antigens that were recognized by at least 2 different monoclonal antibodies (mAbs). The proteins that were antigenically dominant were: acetyl-Coenzyme A acyltransferase 2 (mitochondrial 3-oxoacyl-Coenzyme A thiolase), aldehyde dehydrogenase 1 family member A1, carbamoyl phosphate synthetase 1, dihydrolipoamide S-acetyltransferase (E2 component of pyruvate dehydrogenase complex), enoyl coenzyme A hydratase 1, and hydroxysteroid (11-beta) dehydrogenase 1. We also determined the subcellular localizations of these enzymes within the mitochondria using immunohistocytochemistry. We believe that these well-characterized antibodies will provide a valuable resource for the Human Liver Proteome Project (HLPP), and will make studies aimed at investigating liver mitochondrial function far easier to perform in future. Our results provide strong evidence that, (i) depletion of dominant proteins from liver mitochondrial samples is possible and, (ii) the approaches adopted in this study can be used to explore or validate protein-protein interactions in this important organelle. | JU YanFang YANG JinJu LIU Rong LIU XiaoLan DU XueMei LIU Li CHEN ZhiCheng CHI Jun LIU ShuEr GAO Yuan GAO JianEn JIAO ShunChang HE FuChu SUN QiHong | 2011 | Science China(Life Sciences)2011，54，1： | 6 |
| 20 | Comparative genomic and transcriptomic analyses uncover the molecular basis of high nitrogen-use efficiency in the wheat cultivar Kenong 9204显示文摘Studying the regulatory mechanisms that drive nitrogen-use efficiency(NUE)in crops is important for sustainable agriculture and environmental protection.In this study,we generated a high-quality genome assembly for the high-NUE wheat cultivar Kenong 9204 and systematically analyzed genes related to nitrogen uptake and metabolism.By comparative analyses,we found that the high-affinity nitrate transporter gene family had expanded in Triticeae.Further studies showed that subsequent functional differentiation endowed the expanded family members with saline inducibility,providing a genetic basis for improving the adaptability of wheat to nitrogen deficiency in various habitats.To explore the genetic and molecular mechanisms of high NUE,we compared genomic and transcriptomic data from the high-NUE cultivar Kenong 9204(KN9204)and the low-NUE cultivar Jing 411 and quantified their nitrogen accumulation under high-and low-nitrogen conditions.Compared with Jing 411,KN9204 absorbed significantly more nitrogen at the reproductive stage after shooting and accumulated it in the shoots and seeds.Transcriptome data analysis revealed that nitrogen deficiency clearly suppressed the expression of genes related to cell division in the young spike of Jing 411,whereas this suppression of gene expression was much lower in KN9204.In addition,KN9204 maintained relatively high expression of NPF genes for a longer time than Jing 411 during seed maturity.Physiological and transcriptome data revealed that KN9204 was more tolerant of nitrogen deficiency than Jing 411,especially at the reproductive stage.The high NUE of KN9204 is an integrated effect controlled at different levels.Taken together,our data provide new insights into the molecular mechanisms of NUE and important gene resources for improving wheat cultivars with a higher NUE trait. | Xiaoli Shi Fa Cui Xinyin Han Yilin He Long Zhao Na Zhang Hao Zhang Haidong Zhu Zhexin Liu Bin Ma Shusong Zheng Wei Zhang Jiajia Liu Xiaoli Fan Yaoqi Si Shuiquan Tian Jianqing Niu Huilan Wu Xuemei Liu Zhuo Chen Deyuan Meng Xiaoyan Wang Liqiang Song Lijing Sun Jie Han Hui Zhao Jun Ji Zhiguo Wang Xiaoyu He Ruilin Li Xuebin Chi Chengzhi Liang Beifang Niu Jun Xiao Junming Li Hong-Qing Ling | 2022 | Molecular Plant2022，15，9： | 6 |
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