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| 1 | The human long non-coding RNA-RoR is a p53 repressor in response to DNA damage显示文摘在应力之上,肿瘤 suppressor p53 的水平显著地被提高,是众所周知的。然而尽管有广泛的研究,包含的内在的机制重要为导致压力的 p53 规定内部播放器仍然不充分被理解。我们人的 lincRNA-RoR (RoR ) 是 p53 的一个强壮的否定管理者的现在的证据。不同于通过 ubiquitin-proteasome 小径引起 p53 降级的 MDM2, RoR 通过和异构的原子 ribonucleoprotein 的直接相互作用压制 p53 翻译我(hnRNP 我) 。重要地,带 hnRNP 的一个 28 底 RoR 序列我有约束力的主题为 p53 压抑必要、足够。我们进一步证明 RoR 禁止调停 p53 的房间周期拘捕和 apoptosis。最后,我们表明 p53 transcriptionally 导致 RoR 表示的一个 RoR-p53 自动调整的反馈环。一起,这些结果建议 RoR-hnRNP I-p53 轴可以组成一个另外的监视网络让房间更好对各种各样的压力作出回应。 | Ali Zhang Nanjiang Zhou Jianguo Huang Qian Liu Koji Fukuda Ding Ma Zhaohui Lu Cunxue Bai Kounosuke Watabe Yin-Yuan Mo | 2013 | Cell Research2013，23，3： | 47 |
| 2 | Promoter methylation and mRNA expression of DKK-3 and WIF-1 in hepatocellular carcinoma显示文摘AIM:To investigate the promoter methylation status and mRNA expression of DKK-3 and WIF-1 gene in hepatocellular carcinoma(HCC).METHODS:DKK-3 and WIF-1 acted as Wnt-antagonists and tumor suppressors,but hypermethylation of the gene promoter and low mRNA expression activated Wnt signaling aberrantly and induced the development of HCC.Methylation status of the DKK-3 and WIF-1 gene promoter was investigated using methylation specific polymerase chain reaction(PCR) in tumor and adjacent non-cancerous tissues from 33 HCC patients and 20 normal liver tissues served as control.The expression of DKK-3 and WIF-1 mRNA was also determined by real-time quantitative reverse transcriptase PCR.The relationship between methylation,mRNA expression,and clinical data,as well as methylation and mRNA expression of the two genes were analyzed.RESULTS:The methylation of DKK-3 and WIF-1 genes in HCC increased significantly compared with adjacent non-cancerous tissues and normal control tissues(χ2 =7.79,P < 0.05;χ2 = 4.89,P < 0.05),and no significant difference in methylation between adjacent non-cancerous tissues and normal control tissues was observed.In HCC tissues,significant differences in the DKK-3 promoter methylation were observed in age and cirrhosis,and significant differences of the WIF-1 promoter methylation were observed in HBsAg and cirrhosis.The average expression of DKK-3 mRNA in HCC and adjacent non-cancerous tissues was increased significantly compared with normal control tissues.The average expression of WIF-1 mRNA showed no significant difference among the three tissues.The mRNA expression of DKK-3 gene in HCC was decreased as the pathological grade increased.CONCLUSION:The aberrant promoter methylation and decreased expression of DKK-3 and WIF-1 may be an important mechanism in HCC,and may be a far-reaching significance in early diagnosis and therapy of HCC. | Zhen Ding Ye-Ben Qian Li-Xin Zhu Qi-Ru Xiong | 2009 | World Journal of Gastroenterology2009，15，21： | 22 |
| 3 | Radiofrequency ablation of hepatocellular carcinoma sized> 3 and ≤ 5 cm: Is ablative margin of more than 1 cm justified?显示文摘AIM:To investigate whether an ablative margin(AM)>1.0 cm might reduce chance of recurrence for patients with hepatocellular carcinoma(HCC)tumors 3.1to 5.0 cm in size,compared with an AM of 0.5-1.0 cm.METHODS:From October 2005 to December 2012,936 consecutive patients with HCC who received radiofrequency ablation were screened.Of these,281 patients,each with a single primary HCC tumor of 3.1 to5.0 cm in size on its greatest diameter,were included in the study.Based on the AM width,we categorized patients into the 0.5-1.0 cm group and the>1.0 cm group.Local tumor progression(LTP)-free survival,intrahepatic distant recurrence(IDR)-free survival and overall survival(OS)rates were obtained using the Kaplan-Meier method.RESULTS:The 1-,2-,3-,4-,and 5-year LTP-free survival rates and IDR-free survival rates were significantly higher in the>1.0 cm group compared with the 0.5-1.0cm group(97.5%,86.3%,73.6%,49.5%and 26.4%vs 91.3%,78.4%,49.5%,27.8%,and 12.8%;95.1%,90.3%,77.0%,61.0%and 48.3%vs 95.2%,85.9%,62.6%,47.2%and 28.5%;P<0.05).The 1-,2-,3-,4-,and 5-year OS rates were 98.6%,91.5%,69.2%,56.0%and 42.2%,respectively,in the 0.5-1.0 cm group and 100%,98.9%,90.1%,68.7%and 57.4%,respectively,in the>1.0 cm group(P=0.010).There were no significant differences in complication rates between the two groups.Both univariate and multivariate analyses identified AM as an independent prognostic factor linked to LTP,IDR,and OS.CONCLUSION:For HCC tumors>3.0 cm and≤5.0cm,AM>1.0 cm could reduce chances of recurrence compared with AM of 0.5-1.0 cm,emphasizing the need for a more defensive strategy using AMs>1.0 cm for ablating HCC tumors of 3.1 to 5.0 cm. | Shan Ke Xue-Mei Ding Xiao-Jun Qian Yi-Ming Zhou Bao-Xin Cao Kun Gao Wen-Bing Sun | 2013 | World Journal of Gastroenterology2013，19，42： | 21 |
| 4 | Precise nanomedicine for intelligent therapy of cancer显示文摘Precise nanomedicine has been extensively explored for efficient cancer imaging and targeted cancer therapy, as evidenced by a few breakthroughs in their preclinical and clinical explorations. Here, we demonstrate the recent advances of intelligent cancer nanomedicine, and discuss the comprehensive understanding of their structure-function relationship for smart and efficient cancer nanomedicine including various imaging and therapeutic applications, as well as nanotoxicity. In particular, a few emerging strategies that have advanced cancer nanomedicine are also highlighted as the emerging focus such as tumor imprisonment, supramolecular chemotherapy, and DNA nanorobot. The challenge and outlook of some scientific and engineering issues are also discussed in future development. We wish to highlight these new progress of precise nanomedicine with the ultimate goal to inspire more successful explorations of intelligent nanoparticles for future clinical translations. | Huabing Chen Zhanjun Gu Hongwei An Chunying Chen Jie Chen Ran Cui Siqin Chen Weihai Chen Xuesi Chen Xiaoyuan Chen Zhuo Chen Baoquan Ding Qian Dong Qin Fan Ting Fu Dayong Hou Qiao Jiang Hengte Ke Xiqun Jiang Gang Liu Suping Li Tianyu Li Zhuang Liu Guangjun Nie Muhammad Ovais Daiwen Pang Nasha Qiu Youqing Shen Huayu Tian Chao Wang Hao Wang Ziqi Wang Huaping Xu Jiang-Fei Xu Xiangliang Yang Shuang Zhu Xianchuang Zheng Xianzheng Zhang Yanbing Zhao Weihong Tan Xi Zhang Yuliang Zhao | 2018 | Science China Chemistry2018，61，12： | 19 |
| 5 | An HNF 1α-regulated feedback circuit modulates hepatic fibrogenesis via the crosstalk between hepatocytes and hepatic stellate cells显示文摘 | Hui Qian Xing Deng Zhao-Wei Huang Ji Wei Chen-Hong Ding Ren-Xin Feng Xin Zeng Yue-Xiang Chen Jin Ding Lei Qiu Zhen-Lin Hu Xin Zhang Hong-Yang Wang Jun-Ping Zhang Wei-Fen Xie | 2015 | Cell Research2015，25，8： | 15 |
| 6 | Intelligent and efficient development of wireless networks:A review of cognitive radio networks显示文摘Facing the challenges on how to improve spectrum efficiency and how to realize heterogeneous network convergence in future wireless networks,a cognitive radio network(CRN) is proposed as one of the solutions.This has become a major research topic in recent years and it is timely to give an overview of the development of CRN and to summarize key issues and technologies.The fundamental concepts of CRN,including the cognitive cycle model,the network architecture,and the cognitive ability and intelligent decision functions,are introduced in detail based on recent advances.Key issues for each topic,followed with recent research on theory and method,are then classified and the industrialization developments of CRN testbeds based on TD-LTE cellular system and standards are briefly presented.Finally,conclusions are reached on the perspectives and directions of future development. | ZHANG Ping LIU Yang FENG ZhiYong ZHANG QiXun LI Qian XU Ding | 2012 | Chinese Science Bulletin2012，57，28： | 15 |
| 7 | Correlation between women's sub-health and reproductive diseases with pregnancies and labors显示文摘OBJECTIVE: To investigate whether female sub-health conditions and reproductive diseases areassociated with pregnancies and labors.METHODS: A cross-sectional survey was performed by using a structured questionnaire. A total of 1343 women aged 35 years or younger in six urban areas of Chengdu were included in the study.According to the Screening Criteria of sub-health conditions, these women were categorized into three groups: postpartum healthy group,sub-healthy group, and reproductive disease group. Data were double-entered using EpiData and the nanalyzed by SPSS.RESULTS: Pregnancy and labor were correlated with postpartum sub-health conditions. The number of pregnancies was negatively correlated with women's postnatal health but was positively correlated with the incidence of postpartum reproductive diseases.CONCLUSION: The number of pregnancies and labors is probably an important factor leading to sub-health conditions and the occurrence of reproductive diseases in women. Avoiding or reducing unwanted pregnancies and labors, enhancing the awareness of health among child bearing-age women are effective measures for preventing sub-health conditions. | Xiaojuan Xu Qian Zeng Hong Ding Lingyan Feng Linwen Deng | 2014 | Journal of Traditional Chinese Medicine2014，34，4： | 14 |
| 8 | Leukotriene D4 induces brain edema and enhances CysLT2 receptor- mediated aquaporin 4 expression显示文摘 | Wang Meng - Ling Yuan Yu - Mei Huang Xiao - Jia Fang San - Hua Zhang Wei-Ping Lu Yu-Bi Ding Qian Wei Er-Qing | 2006 | 中国药理通讯2006，23，4： | 14 |
| 9 | Role of gut microbiota via the gut-liver-brain axis in digestive diseases显示文摘The gut-brain axis is a bidirectional information interaction system between the central nervous system(CNS) and the gastrointestinal tract, in which gut microbiota plays a key role. The gut microbiota forms a complex network with the enteric nervous system, the autonomic nervous system, and the neuroendocrine and neuroimmunity of the CNS, which is called the microbiota-gut-brain axis. Due to the close anatomical and functional interaction of the gut-liver axis, the microbiota-gut-liver-brain axis has attracted increased attention in recent years. The microbiota-gut-liver-brain axis mediates the occurrence and development of many diseases, and it offers a direction for the research of disease treatment. In this review, we mainly discuss the role of the gut microbiota in the irritable bowel syndrome, inflammatory bowel disease, functional dyspepsia, non-alcoholic fatty liver disease, alcoholic liver disease, cirrhosis and hepatic encephalopathy via the gut-liver-brain axis, and the focus is to clarify the potential mechanisms and treatment of digestive diseases based on the further understanding of the microbiota-gut-liver-brain axis. | Jian-Hong Ding Zhe Jin Xiao-Xu Yang Jun Lou Wei-Xi Shan Yan-Xia Hu Qian Du Qiu-Shi Liao Rui Xie Jing-Yu Xu | 2020 | World Journal of Gastroenterology2020，26，40： | 13 |
| 10 | The earthquake-related disturbances in ionosphere and project of the first China seismo-electromagnetic satellite显示文摘Based on the case studies and statistical analysis of earthquake-related ionospheric disturbances mainly from DEMETER satellite,ground-based GPS and ionosounding data,this paper summarizes the statistical characteristics of earthquake-related ionospheric disturbances,including electromagnetic emissions,plasma perturbations and variation of energetic particle flux.According to the main results done by Chinese scientists, fusing with the existed study from global researches,seismo-ionospheric disturbances usually occurred a few days or hours before earthquake occurrence.Paralleling to these case studies,lithosphere-atmosphere-ionosphere (LAI) coupling mechanisms are checked and optimized.A thermo-electric model was proposed to explain the seismo-electromagnetic effects before earthquakes.A propagation model was put forward to explain the electromagnetic waves into the ionosphere.According to the requirement of earthquake prediction research,China seismo-electromagnetic satellite,the first space-based platform of Chinese earthquake stereoscopic observation system,is proposed and planned to launch at about the end of 2014.It focuses on checking the LAI model and distinguishing earthquake-related ionospheric disturbance.The preliminary design for the satellite will adopt CAST-2000 platform with eight payloads onboard.It is believed that the satellite will work together with the ground monitoring network to improve the capability to capture seismo-electromagnetic information,which is beneficial for earthquake monitoring and prediction researches. | Xuhui Shen Xuemin Zhang Lanwei Wang Huaran Chen Yun Wu Shigeng Yuan Junfeng Shen Shufan Zhao Jiadong Qian Jianhai Ding | 2011 | Earthquake Science2011，24，6： | 13 |
| 11 | GSA:Genome Sequence Archive显示文摘With the rapid development of sequencing technologies towards higher throughput and lower cost, sequence data are generated at an unprecedentedly explosive rate. To provide an efficient and easy-to-use platform for managing huge sequence data, here we present Genome Sequence Archive(GSA; http://gffzzdbc7b6aaae734bddh96xqxvcoxofk6cpq.ffgz.tsg.suse.edu.cn/gsa or http://gffzz4eaf941a184140e3h96xqxvcoxofk6cpq.ffgz.tsg.suse.edu.cn), a data repository for archiving raw sequence data. In compliance with data standards and structures of the International Nucleotide Sequence Database Collaboration(INSDC), GSA adopts four data objects(Bio Project, Bio Sample,Experiment, and Run) for data organization, accepts raw sequence reads produced by a variety of sequencing platforms, stores both sequence reads and metadata submitted from all over the world,and makes all these data publicly available to worldwide scientific communities. In the era of big data, GSA is not only an important complement to existing INSDC members by alleviating the increasing burdens of handling sequence data deluge, but also takes the significant responsibility for global big data archive and provides free unrestricted access to all publicly available data in support of research activities throughout the world. | Yanqing Wang Fuhai Song Junwei Zhu Sisi Zhang Yadong Yang Tingting Chen Bixia Tang Lili Dong Nan Ding Qian Zhang Zhouxian Bai Xunong Dong Huanxin Chen Mingyuan Sun Shuang Zhai Yubin Sun Lei Yu Li Lan Jingfa Xiao Xiangdong Fang Hongxing Lei Zhang Zhang Wenming Zhao | 2017 | Genomics, Proteomics & Bioinformatics2017，15，1： | 13 |
| 12 | Clinical characteristics of human infection with a novel avian-origin influenza A(H10N8) virus显示文摘 | Zhang Wei Wan Jianguo Qian Kejian Liu Xiaoqing Xiao Zuke Sun Jian Zeng Zhenguo Wang Qi Zhang Jinxiang Jiang Guanghui Nie Cheng Jiang Rong Ding Chengzhi Li Ran Horby Peter Gao Zhancheng | 2014 | Chinese Medical Journal2014，，18： | 12 |
| 13 | Carvedilol may attenuate liver cirrhosis by inhibiting angiogenesis through the VEGF-Src-ERK signaling pathway显示文摘AIM: To investigate the effect of carvedilol on angiogenesis and the underlying signaling pathways.METHODS: The effect of carvedilol on angiogenesis was examined using a human umbilical vascular endothelial cell(HUVEC) model. The effect of carvedilol on cell viability was measured by CCK8 assay. Flow cytometry was used to assess the effect of carvedilol on cell cycle progression. Cell migration, transwell migration and tube formation assays were performed to analyze the effect of carvedilol on HUVEC function. Vascular endothelial growth factor(VEGF) induced activation of HUVECs, which were pretreated with different carvedilol concentrations or none. Western blot analysis detected the phosphorylation levels of three cell signaling pathway proteins, VEGFR-2, Src, and extracellular signal-regulated kinase(ERK). The specific Src inhibitor PP2 was used to assess the role of Src in the VEGF-induced angiogenic pathway.RESULTS: Carvedilol inhibited HUVEC proliferation in a dose-dependent manner(IC50 = 38.5 mmol/L). The distribution of cells in the S phase decreased from 43.6% to 37.2%, 35.6% and 17.8% by 1, 5 and 10 μmol/L carvedilol for 24 h, respectively. Carvedilol(10 μmol/L) reduced VEGF-induced HUVEC migration from 67.54 ± 7.83 to 37.11 ± 3.533(P < 0.001). Carvedilol concentrations of 5 μmol/L and 10 μmol/L reduced cell invasion from 196.3% ± 18.76% to 114.0% ± 12.20% and 51.68% ± 8.28%, respectively. VEGFinduced tube formation was also reduced significantly by 5 μmol/L and 10 μmol/L carvedilol from 286.0 ± 36.72 to 135.7 ± 18.13(P < 0.05) and 80.27 ± 11.16(P < 0.01) respectively. We investigated several intracellular protein levels to determine the reason for these reductions. Treatment with 10 μmol/L carvedilol reduced VEGF-induced tyrosine phosphorylation of VEGFR-2 from 175.5% ± 8.54% to 52.67% ± 5.33%(P < 0.01). Additionally, 10 μmol/L carvedilol reduced VEGF-induced ERK 1/2 phosphorylation from 181.9% ± 18.61% to 56.45% ± 7.64%(P < 0.01). The VEGFinduced increase in Src kinase activity was alleviated by carvedilol [decreased from 141.8% ± 15.37% to 53.57 ± 7.18%(P < 0.01) and 47.04% ± 9.74%(P < 0.01) at concentrations of 5 and 10 μmol/L, respectively]. Pretreatment of HUVECs with Src kinase inhibitor almost completely prevented the VEGF-induced ERK upregulation [decreased from 213.2% ± 27.68% to 90.96% ± 17.16%(P < 0.01)].CONCLUSION: Carvedilol has an anti-angiogenic effect on HUVECs. This inhibitory effect is mediated by VEGF-induced Src-ERK signaling pathways. | Qian Ding Xiang-Guo Tian Yan Li Qi-Zhi Wang Chun-Qing Zhang | 2015 | World Journal of Gastroenterology2015，21，32： | 12 |
| 14 | Characteristics and Changes of Cold Surge Events over China during 1960-2007显示文摘This paper demonstrates regional characteristics, a long-term decreasing trend, and decadal variations in the frequency of cold surge events based on daily mean temperature and daily minimum temperature data in China's Mainland from 1960 to 2008. During these 48 years four high frequency centers of cold surge events were located in Xinjiang, central North China, northeast China, and southeast China. A main frequency peak of cold surge events occurs in autumn for the four regions and another peak is detected in spring over northeast China and southeast China. The regional pattern of cold surge frequencies is in accordance with the perturbation kinetic energy distribution in October December, January, and February April. The long-term decreasing trend ( 0.2 times/decade) of cold surge frequencies in northeast China and decadal variations in China are related to the variations of the temperature difference between southern and northern China in the winter monsoon season; these variations are due to the significant rising of winter temperatures in high latitudes. | DING Ting QIAN Wei-Hong YAN Zhong-Wei | 2009 | Atmospheric and Oceanic Science Letters2009，2，6： | 11 |
| 15 | Transcriptional analysis and histochemistry reveal that hypersensitive cell death and H2O2 have crucial roles in the resistance of tea plant (Camellia sinensis (L.) O. Kuntze) to anthracnose显示文摘Anthracnose causes severe losses of tea production in China.Although genes and biological processes involved in anthracnose resistance have been reported in other plants,the molecular response to anthracnose in tea plant is unknown.We used the susceptible tea cultivar Longjing 43 and the resistant cultivar Zhongcha 108 as materials and compared transcriptome changes in the leaves of both cultivars following Colletotrichum fructicola inoculation.In all,9015 and 8624 genes were differentially expressed between the resistant and susceptible cultivars and their controls(0 h),respectively.In both cultivars,the differentially expressed genes(DEGs)were enriched in 215 pathways,including responses to sugar metabolism,phytohormones,reactive oxygen species(ROS),biotic stimuli and signalling,transmembrane transporter activity,protease activity and signalling receptor activity,but DEG expression levels were higher in Zhongcha 108 than in Longjing 43.Moreover,functional enrichment analysis of the DEGs showed that hydrogen peroxide(H2O2)metabolism,cell death,secondary metabolism,and carbohydrate metabolism are involved in the defence of Zhongcha 108,and 88 key genes were identified.Protein–protein interaction(PPI)network demonstrated that putative mitogen-activated protein kinase(MAPK)cascades are activated by resistance(R)genes and mediate downstream defence responses.Histochemical analysis subsequently validated the strong hypersensitive response(HR)and H2O2 accumulation that occurred around the hyphal infection sites in Zhongcha 108.Overall,our results indicate that the HR and H2O2 are critical mechanisms in tea plant defence against anthracnose and may be activated by R genes via MAPK cascades. | Yuchun Wang Xinyuan Hao Qinhua Lu Lu Wang Wenjun Qian Nana Li Changqing Ding Xinchao Wang Yajun Yang | 2018 | Horticulture Research2018，5，1： | 11 |
| 16 | Snail and Slug mediate tamoxifen resistance in breast cancer cells through activation of EGFR-ERK independent of epithelial-mesenchymal transition显示文摘 | Yan Jian Xiaotong Zhao Qian Xiao Qingbo Liu Keshuo Ding Fei Yu Rui Zhang Tao Zhu Gaoxiang Ge | 2014 | Journal of Molecular Cell Biology2014，8，4： | 11 |
| 17 | ELE restrains empty glumes from developing into lemmas显示文摘Although there is evident homology among reproductive organs when comparing Poaceae (grass) and eudicots, the identity of grass specific organs, such as lodicules, palea, lemma, and glumes has been the subject of a vast and largely inconclusive discussion. Here we provide some direct evidence to support the idea that the empty glumes of rice (Oryza sativa) are counterparts of lemmas. We show that the development of empty glumes is regulated by ELE (elongated empty glume), which belongs to a plant specific novel gene family. Mutations at the ELE locus cause elongated empty glumes, which mimic the lemmas and have the epidermal morphology of lemmas with four or five vascular bundles. As a nuclear-localized gene, ELE is specifically expressed at the empty glumes of immature spikelets, and its ectopic expression causes many floral development defects, including lemma-like palea, extra palea-like structures, elongated lodicules, extra stamens and stigmas. Our result suggests that empty glumes are lemmas of the sterile florets located at the lateral side of the rice spikelet, and ELE acts as a regulator restraining its growth to maintain its small size in wild-type plants. | Lilan Hong Qian Qian Keming Zhu Ding Tang Zejun Huang Ling Gao Ming Li Minghong Gu Zhukuan Cheng | 2010 | Journal of Genetics and Genomics2010，37，2： | 11 |
| 18 | Shielding of the geomagnetic field reduces hydrogen peroxide production in human neuroblastoma cell and inhibits the activity of CuZn superoxide dismutase显示文摘积聚的证据证明了一块地磁气的地的不利效果防护了状况因此打电话给一块 hypomagnetic 地(HMF ) ,在在动物和房间的新陈代谢的过程和氧化应力上。然而,内在的机制仍然保持不清楚。在这研究,我们在人的 neuroblastoma SH-SY5Y 房间在细胞的反应的氧种类(ROS ) 的规定上评估 HMF 的角色。我们发现 HMF 暴露导致了 ROS 减少,并且那恢复由另外的 H 2 O 2 救了提高 HMF 的房间增长。ROS 上的大小联系了索引,包括全部的抗氧化剂能力, H 2 O 2 和 superoxide 阴离子层次,和 superoxide dismutase (草皮) 活动和表示,显示 HMF 减少了 H 2 O 2 生产并且禁止了 CuZn 草皮的活动。而且, HMF 象 CuZn 草皮蛋白质的提高的聚集一样加速了 CuZn 草皮的变性作用,在 vitro。我们的调查结果显示 CuZn 草皮对对 HMF 压力的反应有能力并且建议它 HMF 效果的一个调停人。 | Hai-tao Zhang Zi-jian Zhang Wei-chuan Mo Ping-dong Hu Hai-min Ding Ying Liu Qian Hua Rong-qiao He | 2017 | Protein & Cell2017，8，7： | 10 |
| 19 | Influence of adriamycin on changes in Nanog, Oct-4, Sox2, ARID1 and Wnt5b expression in liver cancer stem cells显示文摘AIM:To determine the influence of Adriamycin(ADM)on the changes in Nanog,Oct4,Sox2,as well as,in ARID1 and Wnt5b expression in liver cancer stem cells.METHODS:The MHCC97-L and HCCLM3 liver cancer cell lines were selected as the cell models in this study,and were routinely cultured.The 50%lethal dose(LD50)in the cell lines was detected by the MTT assay.Expression changes in liver cancer stem cell related genes(Nanog,Oct-4,Sox2,ARID1,and Wnt5b)were detected by western blot following treatment with ADM(LD50).RESULTS:The LD50 of ADM in MHCC97-L cells was lower than that in HCCLM3 cells(0.4123±0.0236μmol/L vs 0.5259±0.0125μmol/L,P<0.05).Wnt5b and Nanog were expressed in both MHCC97-L and HCCLM3 cells,while only Sox2 was expressed in HCCLM3cells.However,neither ARID1A nor Oct4 was detected in these two cell lines.Genes,related to the stem cells,showed different expression in liver cancer cells with different metastatic potential following treatment with ADM(LD50).Wnt5b protein increased gradually within4 h of ADM(LD50)treatment,while Nanog decreased(P<0.05).After 12 h,Wnt5b decreased gradually,while Nanog increased steadily(P<0.05).In addition,only Sox2 was expressed in HCCLM3 cells with high metastatic potential following ADM(LD50)treatment.The expression of Sox2 increased gradually with ADM(LD50)in HCCLM3 cells(P<0.05).CONCLUSION:ADM increased the death rate of MHCC97-L and HCCLM3 cells,while the growth suppressive effect of ADM was higher in MHCC97-L cells than in HCCLM3 cells. | Ding Sun Lei Qin Yang Xu Jian-Xia Liu Li-Ping Tian Hai-Xin Qian | 2014 | World Journal of Gastroenterology2014，20，22： | 10 |
| 20 | Two New Flavonoid Glycosides from Chrysanthemum morifolium显示文摘Two new flavonoid glycosides were isolated from the flowering heads of Chrysanthemum morifolium. Their structures were determined to be luteolin 4’-methoxy-7- O-(6'-O-acetyl)-β-D-glucopyranoside (1) and acacetin 7-O-(3'-O-acetyl)-β-D-glucopyranoside (2) by means of 1H and 13C NMR spectroscopic analysis, including 2D NMR technique. | Jian ZHANG An Wei DING You Bin LI Da Wei QIAN Jin Ao DUAN Zhi Qi YIN | 2006 | Chinese Chemical Letters2006，17，8： | 10 |
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