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| 1 | Paris saponin Ⅶ,a direct activator of AMPK,induces autophagy and exhibits therapeutic potential in non-small-cell lung cancer显示文摘Paris saponinⅦ(PSⅦ),a bioactive constituent extracted from Trillium tschonoskii Maxim.,is cytotoxic to several cancer types.This study was designed to explore whether PSⅦprevents non-small-cell lung cancer(NSCLC)proliferation and to investigate its molecular target.AMP-activated protein kinase(AMPK)has been implicated in the activation of autophagy in distinct tissues.In cultured human NSCLC cell lines,PSⅦinduces autophagy by activating AMPK and inhibiting m TOR signaling.Furthermore,PSⅦ-induced autophagy activation was reversed by the AMPK inhibitor compound C.Computational docking analysis showed that PSⅦdirectly interacted with the allosteric drug and metabolite site of AMPK to stabilize its activation.Microscale thermophoresis assay and drug affinity responsive target stability assay further confirmed the high affinity between PSⅦand AMPK.In summary,PSⅦacts as a direct AMPK activator to induce cell autophagy,which inhibits the growth of NSCLC cells.In the future,PSⅦtherapy should be applied to treat patients with NSCLC. | XIANG Yu-Chen SHEN Jie SI Yuan LIU Xue-Wen ZHANG Liang WEN Jun ZHANG Te YU Qing-Qing LU Jun-Fei XIANG Ke LIU Ying | 2021 | Chinese Journal of Natural Medicines2021，19，3： | 4 |
| 2 | Single amino acid substitution of VP1 N17D or VP2 H145Y confers acid-resistant phenotype of type Asia1 foot-and-mouth disease virus显示文摘Infection by foot-and-mouth disease virus(FMDV) is triggered by the acidic pH in endosomes after virus uptake by receptor-mediated endocytosis. However, dissociation of the FMDV 146S particle in mildly acidic conditions renders inactivated foot-and-mouth disease(FMD) vaccines much less effective. Type Asia1 FMDV mutants with increased resistance to acid inactivation were selected to study the molecular basis of viral resistance to acid-induced disassembly and improve the acid stability of FMDV. Sequencing of capsid-coding regions revealed four amino acid replacements(VP1 N17D, VP2 H145Y, VP2 G192D, and VP3 K153E) in the viral population of the acid-selected 10th passage. We performed single or combined mutagenesis using a reverse genetic system, and our results provide direct experimental evidence that VP2 H145Y or VP1 N17D substitution confers an acid-resistant phenotype to type Asia1 FMDV. | Haiwei Wang Shanshan Song Jianxiong Zeng Guohui Zhou Decheng Yang Te Liang Li Yu | 2014 | Virologica Sinica2014，29，2： | 2 |
| 3 | TLR cross-talk specifically regu- lates cytokine production by B cells from chronic inflammatory disease patients显示文摘 | Jagannathan M Hasturk H Liang Y Shin H Hetzel JT Kantarci A Ruhin D McDonnell ME Van Dyke TE Gan- ley-Leal LM Nikolaiczyk BS | 2009 | J Immunol2009，183，11： | 1 |
| 4 | Paris saponin VII,a Hippo pathway activator,induces autophagy and exhibits therapeutic potential against human breast cancer cells显示文摘Dysregulation of the Hippo signaling pathway seen in many types of cancer is usually associated with a poor prognosis.Paris saponin VII(PSVII)is a steroid saponin isolated from traditional Chinese herbs with therapeutic action against various human cancers.In this study we investigated the effects of PSVII on human breast cancer(BC)cells and its anticancer mechanisms.We showed that PSVII concentration-dependently inhibited the proliferation of MDA-MB-231,MDA-MB-436 and MCF-7 BC cell lines with IC_(50) values of 3.16,3.45,and 2.86μM,respectively,and suppressed their colony formation.PSVII(1.2-1.8μM)induced caspase-dependent apoptosis in the BC cell lines.PSVII treatment also induced autophagy and promoted autophagic flux in the BC cell lines.PSVII treatment decreased the expression and nuclear translocation of Yes-associated protein(YAP),a downstream transcriptional effector in the Hippo signaling pathway;overexpression of YAP markedly attenuated PSVII-induced autophagy.PSVII-induced,YAP-mediated autophagy was associated with increased active form of LATS1,an upstream effector of YAP.The activation of LATS1 was involved the participation of multiple proteins(including MST2,MOB1,and LATS1 itself)in an MST2-dependent sequential activation cascade.We further revealed that PSVII promoted the binding of LATS1 with MST2 and MOB1,and activated LATS1 in the BC cell lines.Molecular docking showed that PSVII directly bound to the MST2-MOB1-LATS1 ternary complex.Microscale thermophoresis analysis and drug affinity responsive targeting stability assay confirmed the high affinity between PSVII and the MST2-MOB1-LATS1 ternary complex.In mice bearing MDA-MB-231 cell xenograft,administration of PSVII(1.5 mg/kg,ip,4 times/week,for 4 weeks)significantly suppressed the tumor growth with increased pLATS1,LC3-II and Beclin 1 levels and decreased YAP,p62 and Ki67 levels in the tumor tissue.Overall,this study demonstrates that PSVII is a novel and direct Hippo activator that has great potential in the treatment of BC. | Yu-chen Xiang Peng Peng Xue-wen Liu Xin Jin Jie Shen Te Zhang Liang Zhang Fang Wan Yu-liang Ren Qing-qing Yu Hu-zi Zhao Yuan Si Ying Liu | 2022 | Acta Pharmacologica Sinica2022，43，6： | 1 |
| 5 | Repair of double-strand breaks by end joining 显示文摘 | Chiruvella KK Liang Z Wilson TE | 2013 | Cold Spring Harb Perspeet Biol2013，5，01： | 1 |
| 6 | A novel protein, luman/ CREB3 remitment factor, inhibits luman activation of the unfolded protein response 显示文摘 | Audas TE Li Yu Liang Genqing | 2008 | Mol Cell Biol2008，28，12： | 1 |
| 7 | Repair of double-strand breaks by end joining显示文摘 | Chiruvella KK Liang Z Wilson TE | 2013 | Cold Spring Harb Perspect Biol2013，5，01： | 1 |
| 8 | Dynamic landscape mapping of humoral immunity to SARS-CoY-2 identifies non-structural protein antibodies associated with the survival of critical COVID-19 patients显示文摘A comprehensive analysis of the humoral immune response to the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)is essential in understanding COVID-19 pathogenesis and developing antibody-based diagnostics and therapy.In this work,we performed a longitudinal analysis of antibody responses to SARS-CoV-2 proteins in 104 serum samples from 49 critical COVID-19 patients using a peptide-based SARS-CoV-2 proteome microarray.Our data show that the binding epitopes of IgM and IgG antibodies differ across SARS-CoV-2 proteins and even within the same protein. | Linlin Cheng Xiaomei Zhang Yu Chen Dan Wang Dong Zhang Songxin Yan Hongye Wang Meng Xiao Te Liang Haolong Li Meng Xu Xin Hou jiayu Dai Xian Wu Mingyuan Li Minya Lu Dong Wu Ran Tian Jing Zhao Yan Zhang Wei Cao Jinglan Wang Xiaowei Yan Xiang Zhou Zhengyin Liu Yingchun Xu Fuchu He Yongzhe Li Xiaobo Yu Shuyang Zhang | 2021 | Signal Transduction and Targeted Therapy2021，6，9： | 1 |
| 9 | Preclinical studies and clinical evaluation of compounds from the genus epimedium for osteopo- rosis and bone health显示文摘 | Indran IR Liang RL Min TE | 2016 | Pharmacol Ther2016，162，： | 1 |
| 10 | Predictors of health-related quality of life in patients with obstructive sleep apnoea显示文摘 | Ye L Liang ZA Weaver TE | 2008 | J Adv Nurs2008，63，1： | 1 |
| 11 | Altered expression profile of apoptosis-related molecules correlated with clinicopathological factors in non-small-celllung cancer显示文摘 | HuangJQ Liang HL Jin TE | 2015 | Int J Clin Exp Pathol2015，8，11： | 1 |
| 12 | METTL3-mediated m^(6)A modification of HMGA2 mRNA promotes subretinal fibrosis and epithelial-mesenchymal transition显示文摘Subretinal fibrosis is a major cause of the poor visual prognosis for patients with neovascular age-related macular degeneration(nAMD).Myofibroblasts originated from retinal pigment epithelial(RPE)cells through epithelial-mesenchymal transition(EMT)contribute to the fibrosis formation.N^(6)-Methyladenosine(m^(6)A)modification has been implicated in the EMT process and multiple fibrotic diseases.The role of m^(6)A modification in EMT-related subretinal fibrosis has not yet been elucidated.In this study,we found that during subretinal fibrosis in the mouse model of laser-induced choroidal neovascularization,METTL3 was upregulated in RPE cells.Through m^(6)A epitranscriptomic microarray and further verification,high-mobility group AT-hook 2(HMGA2)was identified as thekey downstream target of METTL3,subsequently activating potent EMT-inducing transcription factor SNAIL.Finally,by subretinal injections of adeno-associated virus vectors,we confirmed that METTL3 deficiency in RPE cells could efficiently attenuate subretinal fibrosis in vivo.In conclusion,our present research identified an epigenetic mechanism of METTL3-m^(6)A-HMGA2 in subretinal fibrosis and EMT of RPE cells,providing a novel therapeutic target for subretinal fibrosis secondary to nAMD. | Yuwei Wang Yuhong Chen Jian Liang Mei Jiang Ting Zhang Xiaoling Wan Jiahui Wu Xiaomeng Li Jieqiong Chen Junran Sun Yifan Hu Peirong Huang Jingyang Feng Te Liu Xiaodong Sun | 2023 | Journal of Molecular Cell Biology2023，15，3： | 1 |
| 13 | Service life prediction of existing reinforced concrete bridges exposed to Chloride environment 显示文摘 | Ming - Te Liang Li - Hsien Lin and Chih - Hsin Liang | 2002 | ASCE Journal of Infrastructure Systems2002，8，3： | 1 |
| 14 | SARS-CoV-2 Epitopes following Infection and Vaccination Overlap Known Neutralizing Antibody Sites显示文摘Identification of epitopes targeted following virus infection or vaccination can guide vaccine design and development of therapeutic interventions targeting functional sites,but can be laborious.Herein,we employed peptide microarrays to map linear peptide epitopes(LPEs)recognized following SARS-CoV-2 infetion and vaccination.LPEs detected by nonhuman primate(NHP)and patient IgMs after SARS-CoV-2 infection extensively overlapped,localized to functionally important virus regions,and aligned with reported neutralizing antibody binding sies.Similar LPE overlap occurred atfter infection and vaccination,with LPE clusters specifc to each stimulus,where strong and conserved LPEs mapping to sites known or likely to inhibit spike protein function.Vaccine-specifc LPEs tended to map to sites known or likely to be afected by structural changes induced by the proline substitutions in the mRNA vaccine's S protein.Mapping LPEs to regions of known functional importance in this manner may acelerate vaccine evaluation and discovery of targets for sile secific therapeutic interventions. | Li Yang Te Liang Lane M.Pierson Hongye Wang Jesse K.Fletcher Shu Wang Duran Bao Lii Zhang Zhen Huang Wenshu Zheng Xiaomei Zhang Heewon Park Yuwen Li James E.Robinson Amy K.Fechan Christopher J.Lyon Jing Cao Lisa A.Morici Chenzhong Li Chad J.Roy Xiaobo Yu Tony Hu | 2022 | Research2022，，2： | 0 |
| 15 | Optimal and constant power allocation for joint transmission in HetNet显示文摘This paper investigates the power allocation issues for joint transmission in heterogeneous network(HetNet),which is characterized by severe cross-tier interference.The optimization problem of maximizing the HetNet throughput is formulated.The original problem turns out to be a non-convex problem,the global optima of which cannot be obtained by conventional optimization methods.This paper develops a novel method to achieve the global optima by turning the original problem into quasi-convex problem.In addition,this paper considers a constant power allocation scheme,as a tradeoff between the system throughput and computational complexity.Based on duality gap theory,the bound of constant power allocation scheme is mathematically derived.Numerical results under different system parameters indicate that both the proposed schemes outperform conventional interference coordination schemes. | ZHOU Wen-an XU Yu-jie LIANG Te ZHANG Yi-yu REN Xiao-tao | 2013 | The Journal of China Universities of Posts and Telecommunications2013，20，6： | 0 |
| 16 | Proteome-wide epitope mapping identifies a resource of antibodies for SARS-CoV-2 detection and neutralization显示文摘Dear Editor,Since December 2019,the coronavirus disease 2019(COVID-19)caused by the severe acute respiratory syndrome coronavirus-2(SARS-CoV-2)has become a worldwide pandemic.1 Significant efforts have been made to generate antibodies to help study COVID-19 pathogenesis,perform diagnostic testing,and develop treatment to neutralize SARS-CoV-2 activity.However,the specific sequence of amino acids recognized and bound by an antibody,the'epitope'is unknown for most antibodies.In this study,we developed a high-throughput epitope mapping platform using a peptide-based SARS-CoV-2 proteome microarray.2 to detect the binding epitopes of 57 commercial antibodies to ORFlab,nudeocapsid(N);spike(S),envelop(E),membrane(M),ORF3a,ORF6,ORF7a,and ORF8 proteins(Supplementary Table S1). | Te Liang Mengli Cheng Fei Teng Hongye Wang Yongqiang Deng Jiahui Zhang Chengfeng Qin Shubin Guo Hui Zhao Xiaobo Yu | 2021 | Signal Transduction and Targeted Therapy2021，6，5： | 0 |
| 17 | CorelKit:An Extensible CorelDraw VBA Program for Geoscience Drawing显示文摘CorelKit is developed with the built-in VBA environment of CorelDraw?,and is a new plug-in in CorelDraw for geological and geochemical drawing.CorelKit can help users quickly and easily draw geological and geochemical graphs,such as scatterplot,triangular scatterplot,line chart,histogram,bar chart,box plot,pie graph,etc.In order to adapt to the geological application,the above functions are strengthened.Scatterplot and triangular scatterplot provide nearly 150 common basemaps,covering rock classification,structural environment discrimination,mineral genesis,isotope geochemistry,etc.,for the convenience of the user mapping.Meanwhile,CorelKit further provides a structural joint rose diagram and sulfur isotopic composition diagram.This software also has many practical functions such as Bézier line topology,Multiple Bézier lines close fill,Single inner fill,Correction basemap,Add a compass,Add a scale,which facilitates daily geological drawing.The software has a friendly interface,in both Chinese and English versions,and is suitable for CorelDraw X4 and later versions.We introduced the Bézier line topology to CorelKit for the first time,which is similar to the GIS®topology for geological maps.According to the“GB/T 958-2015 Geological Legends Used for Regional Geological Map”,a two-color filling library of rock pattern pictures is established,which is convenient to realize the twocolor filling of sedimentary rocks,metamorphic rocks and magmatic rocks patterns.Obviously,CorelKit makes up for many shortcomings of CorelDraw in geosciences and is a very practical tool for geoscience researchers. | Junwu Zhang Xian Liang Fangyue Wang Huaikun Wang Yu Fan Te Ba Xiangxi Meng | 2023 | Journal of Earth Science2023，34，3： | 0 |
| 18 | A new strategy for the fabrication of a flexible and highly sensitive capacitive pressure sensor显示文摘The development of flexible capacitive pressure sensors has wide application prospects in the fields of electronic skin and intelligent wearable electronic devices,but it is still a great challenge to fabricate capacitive sensors with high sensitivity.Few reports have considered the use of interdigital electrode structures to improve the sensitivity of capacitive pressure sensors.In this work,a new strategy for the fabrication of a high-performance capacitive flexible pressure sensor based on MXene/polyvinylpyrrolidone(PVP)by an interdigital electrode is reported.By increasing the number of interdigital electrodes and selecting the appropriate dielectric layer,the sensitivity of the capacitive sensor can be improved.The capacitive sensor based on MXene/PVP here has a high sensitivity(~1.25 kPa^(−1)),low detection limit(~0.6 Pa),wide sensing range(up to 294 kPa),fast response and recovery times(~30/15 ms)and mechanical stability of 10000 cycles.The presented sensor here can be used for various pressure detection applications,such as finger pressing,wrist pulse measuring,breathing,swallowing and speech recognition.This work provides a new method of using interdigital electrodes to fabricate a highly sensitive capacitive sensor with very promising application prospects in flexible sensors and wearable electronics. | Ruzhan Qin Mingjun Hu Xin Li Te Liang Haoyi Tan Jinzhang Liu Guangcun Shan | 2021 | Microsystems & Nanoengineering2021，7，6： | 0 |
| 19 | Mechanistic study on the pharmacokinetic process of salidroside in hypoxic rats显示文摘OBJECTIVE To investigate the effect of hypoxia on the pharmacokinetic process of salidrosidein rats and to explore its underlying mechanisms.METHODS The Caco-2 cell monolayer was exposed to 1% oxygen(O_2) concentration for 24 h to build the hypoxiccell model.The transportation mode of salidroside was investigated with the aid of this hypoxia model by detecting the apparent permeability coefficient(P app).Healthy Sprague Dawley(SD) rats were exposed to 9% O_2 for 72 h for the construction of hypoxic rat model.Liver sample was subsequently collected from the hypoxic rats with an aim to identify enzymes responsible for salidroside metabolism.The expression levels of salidroside-transporting and salidroside-metabolizing enzymes,including Sodium-dependent glucose cotransporters(SGLT1),β-glucosidase(GBA3)and sulfotransferase(SULT2A1),were thereafter detected by RT-PCR and Western blot.The metabolic activity of GBA3 and SULT2A1 was monitored by rat liver microsome incubation.In addition,the renal function of rats under hypoxia was assessed by detecting concentrations of blood urea nitrogen and creatinine.RESULTS The AUC and t1/2 values of salidroside in hypoxic rats were more than doubled,while the in vivo clearance was significantly reduced.Mechanistic study demonstrated that the Papp A-B/Papp B-A eualsto 10.3,indicating the potential active transport of salidrosile.The expression of SGLT1 and GBA3 was significantly decreased,which indicated a reduced metabolism of salidroside under hypoxia.Moreover,rat under hypoxia was found to suffer from renal dysfunction,with an abnormal value of blood urea nitrogen.CONCLUSION Due to the reduced metabolism and the abnormal renal function under hypoxia,the systemic exposure of salidroside in rats was significantly enhanced. | Te QI Bei-kang GE Liang ZHAO Ping-xiang XU Ming XUE | 2017 | 中国药理学与毒理学杂志2017，31，10： | 0 |
| 20 | Glucuronidation is the dominating in-vivo metabolism pathway of herbacetin:elucidation of herbacetin pharmacokinetics after intravenous and oral administration in rats显示文摘OBJECTIVE To map a comprehensive metabolic pathway of herbacetin in rats,specifically,to elucidate the biotransformation of herbacetin in vivo and to simultaneously monitor the pharmacokinetic process of both parent drug and its major metabolites.METHODS liquid chromatography/ion trap mass spectrometry(LC/MS n) and ultra-liquid chromatography coupled with mass spectrometry(UPLC/MS) were combined in the current study for qualitative and quantitative determinations of herbacetin and its metabolites in bile,urine and feces after both oral and intravenous administration of herbacetin to rats.Enzyme kinetic studies on the intestinal and hepatic metabolism of herbacetin were further conducted to elucidate metabolic profiles of herbacetin in rat tissues and organs.Additionally,plasma concentration profiles of herbacetin and its metabolites in rats were obtained to characterize the overall pharmacokinetic behavior of herbacetin.RESULTS It was found that herbacetin was excreted primarily from rat urine in the form of glucuronide-conjugations.Subsequent in vitro enzyme kinetic studies and in vivo pharmacokinetic investigations suggested an extensive hepatic metabolism of herbacetin and the high exposure of herbacetin-glucuronides in systemic circulation.The clearance,half-life and bioavailability of herbacetin in rats were determined as(16.4±1.92)mL·kg^(-1)·min^(-1),(11.9±2.7)min,and 1.32%,respectively.On basis of these findings,a comprehensive metabolic pathway of herbacetin in rats was composed.In addition,a physiology based pharmacokinetic(PBPK) model was successfully developed with the aid of the Gastro Plus to simulate the pharmacokinetic process of herbacetin in rats.Application of the PBPK modeling can provide a useful starting point to understand and extrapolate pharmacokinetic parameters among different species,populations,and disease states.CONCLUSION After oral administration,herbacetin was subjected to colonic degradation and extensive first pass metabolism,with glucuronidation as its dominating in vivo metabolic pathway. | Bei-kang GE Liang ZHAO Te QI Ping-xiang XU Ming XUE | 2017 | 中国药理学与毒理学杂志2017，31，10： | 0 |
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