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| 1 | Ythdc2 is an N^6-methyladenosine binding protein that regulates mammalian spermatogenesis显示文摘N 6-methyladenosine (m 6 一) 是在真核细胞的 mRNA 的最普通的内部修正。它动态地被安装并且搬迁,并且充当 mRNA 新陈代谢,包括干细胞 pluripotency 的调整生物过程,房间区别,和精力动态平衡的新层。m 6 A 被选择有约束力的蛋白质认出;YTHDF1 和 YTHDF3 在音乐会工作影响 m 6包含 A 的 mRNAs, YTHDF2 帮助 mRNA 腐烂,和 YTHDC1 影响它的目标的原子处理。YTHDC2 的生物功能, YTH 蛋白质家庭,的最后的成员仍然保持未知。我们报导 YTHDC2 有选择地绑 m 6 在它的一致主题的 A。YTHDC2 提高它的目标的翻译效率并且也减少他们的 mRNA 丰富。Ythdc2 猛烈老鼠是不肥沃的;男性们让显著地更小的睾丸和女性同窝出生的人与那些相比有显著地更小的卵巢。Ythdc2 猛烈老鼠的细菌房间不经过 zygotene 阶段发展,因此,当成熟分裂开始, Ythdc2 是在睾丸的 upregulated。因此, YTHDC2 是 m 6 在精子发生期间起关键作用的 A 绑定蛋白质。 | Phillip J Hsu Yunfei Zhu Honghui Ma Yueshuai Guo Xiaodan Shi Yuanyuan Liu Meijie Qi Zhike Lu Hailing Shi Jianying Wang Yiwei Cheng Guanzheng Luo Qing Dai Mingxi Liu Xuejiang Guo Jiahao Sha Bin Shen Chuan He | 2017 | Cell Research2017，27，9： | 96 |
| 2 | Generation of a precise Oct4-hrGFP knockin cynomolgus monkey model via CRISPR/Cas9-assisted homologous recombination显示文摘 | Yiqiang Cui Yuyu Niu Jiankui Zhou Yongchang Chen Yiwei Cheng Shangang Li Zongyong Ai Chu Chu Hong Wang Bo Zheng Xuejin Chen Jiahao Sha Xuejiang Guo Xingxu Huang Weizhi Ji | 2018 | Cell Research2018，28，3： | 13 |
| 3 | Effects of RapidEye Imagery's Red-edge Band and Vegetation Indices on Land Cover Classification in an Arid Region显示文摘Land cover classification(LCC) in arid regions is of great significance to the assessment, prediction, and management of land desertification. Some studies have shown that the red-edge band of RapidE ye images was effective for vegetation identification and could improve LCC accuracy. However, there has been no investigation of the effects of RapidE ye images' red-edge band and vegetation indices on LCC in arid regions where there are spectrally similar land covers mixed with very high or low vegetation coverage information and bare land. This study focused on a typical inland arid desert region located in Dunhuang Basin of northwestern China. First, five feature sets including or excluding the red-edge band and vegetation indices were constructed. Then, a land cover classification system involving plant communities was developed. Finally, random forest algorithm-based models with different feature sets were utilized for LCC. The conclusions drawn were as follows: 1) the red-edge band showed slight contribution to LCC accuracy; 2) vegetation indices had a significant positive effect on LCC; 3) simultaneous addition of the red-edge band and vegetation indices achieved a significant overall accuracy improvement(3.46% from 86.67%). In general, vegetation indices had larger effect than the red-edge band, and simultaneous addition of them significantly increased the accuracy of LCC in arid regions. | LI Xianju CHEN Gang LIU Jingyi CHEN Weitao CHENG Xinwen LIAO Yiwei | 2017 | Chinese Geographical Science2017，27，5： | 8 |
| 4 | The Chinese Hα Solar Explorer(CHASE) mission: An overview显示文摘The Chinese Hα Solar Explorer(CHASE), dubbed “Xihe”—Goddess of the Sun, was launched on October 14, 2021 as the first solar space mission of China National Space Administration(CNSA). The CHASE mission is designed to test a newly developed satellite platform and to acquire the spectroscopic observations in the Hα waveband. The Hα Imaging Spectrograph(HIS)is the scientific payload of the CHASE satellite. It consists of two observational modes: raster scanning mode and continuum imaging mode. The raster scanning mode obtains full-Sun or region-of-interest spectral images from 6559.7 to 6565.9 ? and from 6567.8 to 6570.6 ? with 0.024 ? pixel spectral resolution and 1 min temporal resolution. The continuum imaging mode obtains photospheric images in continuum around 6689 ? with the full width at half maximum of 13.4 ?. The CHASE mission will advance our understanding of the dynamics of solar activity in the photosphere and chromosphere. In this paper, we present an overview of the CHASE mission including the scientific objectives, HIS instrument overview, data calibration flow, and first results of on-orbit observations. | Chuan Li Cheng Fang Zhen Li MingDe Ding PengFei Chen Ye Qiu Wei You Yuan Yuan MinJie An HongJiang Tao XianSheng Li Zhe Chen Qiang Liu Gui Mei Liang Yang Wei Zhang WeiQiang Cheng JianXin Chen ChangYa Chen Qiang Gu QingLong Huang MingXing Liu ChengShan Han HongWei Xin ChangZheng Chen YiWei Ni WenBo Wang ShiHao Rao HaiTang Li Xi Lu Wei Wang Jun Lin YiXian Jiang LingJie Meng Jian Zhao | 2022 | Science China(Physics,Mechanics & Astronomy)2022，65，8： | 7 |
| 5 | Calibration procedures for the CHASE/HIS science data显示文摘The Hα line is an important optical line in solar observations containing the information from the photosphere to the chromosphere. To study the mechanisms of solar eruptions and the plasma dynamics in the lower atmosphere, the Chinese Hα Solar Explorer(CHASE) was launched into a Sun-synchronous orbit on October 14, 2021. The scientific payload of the CHASE satellite is the Hα Imaging Spectrograph(HIS). The CHASE/HIS acquires, for the first time, seeing-free Hα spectroscopic observations with high spectral and temporal resolutions. It consists of two observational modes. The raster scanning mode provides full-Sun or region-of-interest spectra at Hα(6559.7-6565.9 ?) and Fe I(6567.8-6570.6 ?) wavebands. The continuum imaging mode obtains full-Sun photospheric images at around 6689 ?. In this paper, we present detailed calibration procedures for the CHASE/HIS science data, including the dark-field and flat-field correction, slit image curvature correction, wavelength and intensity calibration, and coordinate transformation. The higher-level data products can be directly used for scientific research. | Ye Qiu ShiHao Rao Chuan Li Cheng Fang MingDe Ding Zhen Li YiWei Ni WenBo Wang Jie Hong Qi Hao Yu Dai PengFei Chen XiaoSheng Wan Zhi Xu Wei You Yuan Yuan HongJiang Tao XianSheng Li YuKun He Qiang Liu | 2022 | Science China(Physics,Mechanics & Astronomy)2022，65，8： | 3 |
| 6 | Breakthrough Technologies for the Biorefining of Organic Solid and Liquid Wastes显示文摘有机固液废弃物包含大量能量、养分和水分,不应仅视其为废物。数十年来,人们一直对城市固体废物进行回收、堆制和燃烧,并从中提取能量和有价值的物质。污水的首要管理策略是治理和排放。随着技术的进步,通过新方法使利用固体废物和废水成为可能。考虑到废物特殊的化学、物理和生物性质,需要整合多种技术以使废物的能量和价值回收最大化。为此,生物炼制是完全利用废物中的能量和价值的一个合适的方法。研究证明,不可回收废弃物和生物固体可通过不同方法转化为可利用的热能、电能、燃料和化学品,并且液体废物或能帮助农作物和藻类的成长,为能量回收和食物生产提供不同的选择。本文针对有机固液废物提出新型生物炼制方案,这些废物来源于城市废料、食品和生物加工厂以及动物生产设施。四大新型突破性科技——真空辅助高温厌氧消化、扩展鱼菜共生系统、含油废物经甘油裂解制取生物柴油和微波辅助热化学转化,均可运用于生物炼制方案中,从而成功将废物转化,用以生产化学品、化肥、能量(沼气、合成气、生物柴油和生物油)、食物、饲料,得到干净的水并显著减少污染物的排放。 | Paul Chen Erik Anderson Min Addy Renchuan Zhang Yanling Cheng Peng Peng Yiwei Ma Liangliang Fan Yaning Zhang Qian Lu Shiyu Liu Nan Zhou Xiangyuan Deng Wenguang Zhou Muhammad Omar Richard Griffith Faryal Kabir Hanwu Lei Yunpu Wang Yuhuan Liu Roger Ruan | 2018 | Engineering2018，4，4： | 3 |
| 7 | PNAS:科学家发现新的精子运动调节器显示文摘南京医科大学生殖医学国家重点实验室、基础医学院组织胚胎学系刘明兮课题组与贝勒医学院MartinM.Matzuk课题组、大阪大学MasahitoIkawa课题组等共同解析了一个精子运动调节基因TCTE1,结果发表于《美国科学院院刊》(PNAs)。 | Castaneda, Julio M. Hua, Rong Miyata, Haruhiko Oji, Asami Guo, Yueshuai Cheng, Yiwei Zhou, Tao Guo, Xuejiang Cui, Yiqiang Shen, Bin Wang, Zibin Hu, Zhibin Zhou, Zuomin Sha, Jiahao Prunskaite-Hyyrylainen, Renata Yu, Zhifeng Ramirez-Solis, Ramiro Ikawa, Masahito Matzuk, Martin M. Liu, Mingxi | 2017 | 现代生物医学进展2017，17，27： | 3 |
| 8 | Personalized cancer vaccines from bacteria-derived outer membrane vesicles with antibody-mediated persistent uptake by dendritic cells显示文摘Nanocarriers with intrinsic immune adjuvant properties can activate the innate immune system while delivering tumor antigen,thus efficiently facilitating antitumor adaptive immunity.Bacteria-derived outer membrane vesicles(OMVs)are an excellent candidate due to their abundance of pathogen associated molecular patterns.However,during the uptake of OMVs by dendritic cells(DCs),the interaction between lipopolysaccharide and toll-like receptor 4 induces rapid DC maturation and uptake blockage,a phenomenon we refer to as“maturation-induced uptake obstruction'(MUO).Herein we decorated OMV with the DC-targeting aDEC205 antibody(OMV-DEC),which endowed the nanovaccine with an uptake mechanism termed as 4 | Jie Liang Keman Cheng Yao Li Jiaqi Xu Yiwei Chen Nana Ma Qingqing Feng Fei Zhu Xiaotu Ma Tianjiao Zhang Yale Yue Guangna Liu Xinjing Guo Zhiqiang Chen Xinwei Wang Ruifang Zhao Ying Zhao Jian Shi Xiao Zhao Guangjun Nie | 2022 | Fundamental Research2022，2，1： | 2 |
| 9 | The C_(6)D_(6)detector system on the Back-n beam line of CSNS显示文摘Introduction The neutron capture cross sections are very important in the field of nuclear device design and basic physics research.Hydrogen-free liquid scintillator such as C_(6)D_(6)detectors are widely used in the neutron capture cross-sectional measurements for the low neutron sensitivity and fast time response.The Back-n white neutron source at China Spallation Neutron Source is the first spallation white neutron source in China,and it is suitable for neutron capture cross-sectional measurement.Materials and methods A C_(6)D_(6)detector system was built in the Back-n experimental station.The pulse height weighting technique was used to determine the system’s detection efficiency.The response to gamma rays of the C_(6)D_(6)detector was measured,and the energy resolution function was determined.Monte Carlo simulation with Geant4 code was carried out to get the weighting function of this C_(6)D_(6)detector system.Additionally,the systematic uncertainty of the weighting function was also determined.Conclusion According to the experimental and simulation results,this C_(6)D_(6)detector system can be used to measure neutron capture cross section. | Jie Ren Xichao Ruan Jie Bao Guangyuan Luan Wei Jiang Qi An Huaiyong Bai Ping Cao Qiping Chen Yonghao Chen Pinjing Cheng Zengqi Cui Ruirui Fan Changqing Feng Minhao Gu Fengqin Guo Changcai Han Zijie Han Guozhu He Yongcheng He Yuefeng He Hanxiong Huang Weiling Huang Xiru Huang Xiaolu Ji Xuyang Ji Haoyu Jiang Hantao Jing Ling Kang Mingtao Kang Bo Li Lun Li Qiang Li Xiao Li Yang Li Yang Li Rong Liu Shubin Liu Xingyan Liu Yinglin Ma Changjun Ning Binbin Qi Zhaohui Song Hong Sun Xiaoyang Sun Zhijia Sun Zhixin Tan Hongqing Tang Jingyu Tang Pengcheng Wang Qi Wang Taofeng Wang Yanfeng Wang Zhaohui Wang Zheng Wang Jie Wen Zhongwei Wen Qingbiao Wu Xiaoguang Wu Xuan Wu Likun Xie Yiwei Yang Han Yi Li Yu Tao Yu Yongji Yu Guohui Zhang Jing Zhang Linhao Zhang Liying Zhang Qingmin Zhang Qiwei Zhang Xianpeng Zhang Yuliang Zhang Zhiyong Zhang Yingtan Zhao Liang Zhou Zuying Zhou Danyang Zhu Kejun Zhu Peng Zhu | 2019 | Radiation Detection Technology and Methods2019，3，3： | 1 |
| 10 | Prevalence of aene vulgaris in Chinese adolescents and a- dults: a community- based study of 17,345 subjects in six cities 显示文摘 | SHEN YIWEI WANG TINGLIN ZHOU CHENG | 2012 | Acta Dermato - Venereologica2012，92，1： | 1 |
| 11 | Power loss transition of stable ZnO varistor ceramics: Role of oxygen adsorption on the stability of interface states at the grain boundary显示文摘Highly stable ZnO varistor ceramics with steadily decreasing power loss have been put into applications in electrical and electronic systems for overvoltage protections, even with the absence of general understandings on their aging behaviors. In this paper, we investigated their aging nature via conducting comparative direct current (DC) aging experiments both in air and in nitrogen, during which variations of electrical properties and interface properties were measured and analyzed. Notably, continuously increasing power loss with severe electrical degradation was observed for the sample aged in nitrogen. The power loss transition was discovered to be closely related to the consumption of oxygen adsorption at the grain boundary (GB), which could, however, remain constant for the sample aged in air. The interface density of states (DOS) Ni, which is crucial for pinning the potential barrier, was proved to decrease in nitrogen, but keep stable in air. Therefore, it is concluded that the oxygen adsorption at the GB is significant for the stability of interface states, which further correlates to the long-term stability of modern stable ZnO varistor ceramics. | Zhuolin Cheng Rou Li Yiwei Long Jianying Li Shengtao Li Kangning Wu | 2023 | Journal of Advanced Ceramics2023，12，5： | 1 |
| 12 | Prevalence of acne vulgaris in Chinese adolescents and adults:a communitybased study of 17,345 subjects in six cities显示文摘 | SHEN Yiwei WANG Tinglin ZHOU Cheng | 2012 | Acta DermatoVenereologica2012，92，1： | 1 |
| 13 | Dynamic Changes of ORF1ab and N Gene Ct Values in COVID-19 Omicron Inpatients of Different Age Groups—Beijing Municipality,China,November-December 2022显示文摘Introduction:In November 2021,the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)Omicron variant was identified as the variant of concern and has since spread globally,replacing other cocirculating variants.To better understand the dynamic changes in viral load over time and the natural history of the virus infection,we analyzed the expression of the open reading frames 1ab(ORF1ab)and nucleocapsid(N)genes in patients infected with Omicron.Methods:We included patients initially admitted to the hospital for SARS-CoV-2 infection between November 5 and December 25,2022.We collected daily oropharyngeal swabs for quantitative reverse transcriptase-polymerase chain reaction tests using commercial kits.We depicted the cycle threshold(Ct)values for amplification of ORF1ab and N genes from individual patients in age-specific groups in a time series.Results:A total of 480 inpatients were included in the study,with a median age of 59 years(interquartile range,42 to 78;range,16 to 106).In the<45-year-old age group,the Ct values for ORF1ab and N gene amplification remained below 35 for 9.0 and 11.5 days,respectively.In the≥80-year-old age group,the Ct values for ORF1ab and N genes stayed below 35 for 11.5 and 15.0 days,respectively,which was the longest among all age groups.The Ct values for N gene amplification took longer to rise above 35 than those for ORF1ab gene amplification.Conclusion:The time to test negative varied among different age groups,with viral nucleic acid shedding taking longer in older age groups compared to younger age groups.As a result,the time to resolution of Omicron infection increased with increasing age. | Qing Zhang Zhaomin Feng Yiwei Hao Lirong Wei Aibin Wang Zhixing Han Meimei Tian Sheng Sun Xin'gang Li Tianjiao Xue Xiangjing Kong Baoliang Li Cheng Kou Quanyi Wang Da Huo Linghang Wang | 2023 | China CDC weekly2023，5，8： | 0 |
| 14 | Ensemble Recurrent Neural Network-Based Residual Useful Life Prognostics of Aircraft Engines显示文摘Residual useful life(RUL)prediction is a key issue for improving efficiency of aircraft engines and reducing their maintenance cost.Owing to various failure mechanism and operating environment,the application of classical models in RUL prediction of aircraft engines is fairly difficult.In this study,a novel RUL prognostics method based on using ensemble recurrent neural network to process massive sensor data is proposed.First of all,sensor data obtained from the aircraft engines are preprocessed to eliminate singular values,reduce random fluctuation and preserve degradation trend of the raw sensor data.Secondly,three kinds of recurrent neural networks(RNN),including ordinary RNN,long shortterm memory(LSTM),and gated recurrent unit(GRU),are individually constructed.Thirdly,ensemble learning mechanism is designed to merge the above RNNs for producing a more accurate RUL prediction.The effectiveness of the proposed method is validated using two characteristically different turbofan engine datasets.Experimental results show a competitive performance of the proposed method in comparison with typical methods reported in literatures. | Jun Wu Kui Hu Yiwei Cheng Ji Wang Chao Deng Yuanhan Wang | 2019 | Structural Durability & Health Monitoring2019，13，3： | 0 |
| 15 | Impact of coal mining on groundwater of Luohe Formation in Binchang mining area显示文摘Groundwater of Luohe Formation is the main water source for industrial and agricultural and residential use in Binchang mining area,which is one of the key elements to water conservation coal mining.However,few studies are available to document the enrichment characteristics and influence of underground coal mining on groundwater for the Luohe Formation.This study evaluates the changes of groundwater levels and spring flow caused by mining activities to explore the influence mechanism of coal mining on groundwater by comparatively analysing existing mining data and survey data combined with a series of mapping methods.The results show that the aquifer of Luohe Formation are gradually thinning south-eastwards,disappeared at the mining boundary.In the vertical direction,the lithological structure is distinct,due to alternative sedimentation of meandering river facies and braided river facies.According to the yielding property,the aquifer is divided into three sections,namely,strong water-rich section,medium water-rich section,and weak water-rich section,which are located in northwest and central part,southwest,and the rest part of the mining area,respectively.Mining of Tingnan Coal Mine since 2004 has caused a 3.16 to 194.87 meters drop in groundwater level of Luohe Formation.Until 2015,70.10%of the mining area undergoes a groundwater level drop larger than 10.00 meters.Another influence of underground mining is that the total flow from 34 springs in 8 southern coal mines of the area has decreased by 286.48 L/s with a rate of decrease at 46.95%from 2007 to 2017.The areas that groundwater level falls or spring flow declines are manly located in the mine gob areas.Results also indicate that the ratio of the height of water conducted fracture zone to the mining height in Binchang mining area is between 16.85 and 27.92.This may increase ground water flow in vertical direction,causing a water level in the aquifer system to drop and ultimately decreasing the flow from the springs.The research results will provide data and theoretical support for the protection of groundwater resources and water conservation coal mining of Luohe Formation in Binchang mining area. | Kui Sun Limin Fan Yucheng Xia Cheng Li Jianping Chen Shuai Gao Boyun Wu Jie Peng Yiwei Ji | 2021 | International Journal of Coal Science & Technology2021，8，1： | 0 |
| 16 | Bubble Consumption Dynamics in Electrochemical Oxygen Reduction显示文摘Electrochemical oxygen reduction reaction(ORR)is crucial for fuel cells and metal-air batteries,while the oxygen consumption dynamics study during ORR,which affects the ORR efficiency,is not as concerned as catalysts design does.Herein the consumption behavior of an individual oxygen bubble on Pt foils with different wettabilities during ORR was tracked by a real-time approach to reveal whether the surface wettability of electrode can influence the consumption dynamics and determine the reaction reactive zones of oxygen bubble consumption.The oxygen bubble underwent a 'constant contact angle'dominant consumption model on aerophobic Pt foil,while an initial 'constant radius'and the subsequent'constant contact angle'oxygen consumption models were observed on aero philic Pt foil.Results here demonstrated that the current was proportional to the bottom contact area,rather than the three-phase contact line of the bubbles according to the fitting curves between individual bubble current and the consumption behavior parameters.This study highlights the important role of the gas-solid interface in influencing the efficiency of gas consumption electrochemical reactions,which shall benefit the understanding of reaction kinetics and the rational design of electrocatalysts. | CHEN Fanhong ZHOU Daojin LU Zhiyi WANG Cheng LUO Liang LIU Yiwei SHANG Zhicheng SHENG Siyu CHENG Congtian XU Haijun SUN Xiaoming | 2020 | Chemical Research in Chinese Universities2020，36，3： | 0 |
| 17 | High-performance broadband flexible photodetector based on Gd_(3)Fe_(5)O_(12)-assisted double van der Waals heterojunctions显示文摘Flexible photodetectors are fundamental components for developing wearable systems,which can be widely used for medical detection,environmental monitoring and flexible imaging.However,compared with 3D materials,lowdimensional materials have degraded performance,a key challenge for current flexible photodetectors.Here,a highperformance broadband photodetector has been proposed and fabricated.By combining the high mobility of graphene(Gr)with the strong light–matter interactions of single-walled carbon nanotubes(SWCNTs)and molybdenum disulfide(MoS2),the flexible photodetector exhibits a greatly improved photoresponse covering the visible to near-infrared range.Additionally,a thin layer of gadolinium iron garnet(Gd_(3)Fe_(5)O_(12),GdlG)film is introduced to improve the interface of the double van der Waals heterojunctions to reduce the dark current.The SWCNT/GdIG/Gr/GdIG/MoS2 flexible photodetector exhibits a high photoresponsivity of 47.375 A/W and a high detectivity of 1.952×1012 Jones at 450 nm,a high photoresponsivity of 109.311 A/W and a high detectivity of 4.504×10^(12) Jones at 1080 nm,and good mechanical stability at room temperature.This work demonstrates the good capacity of GdIGassisted double van der Waals heterojunctions on flexible substrates and provides a new solution for constructing high-performance flexible photodetectors. | Ze Zhang Peirui Ji Shaobo Li Fei Wang Shengmei He Yiwei Cheng Shuhao Zhao Kaili Li Xiaomin Wang Yu Wang Shuming Yang | 2023 | Microsystems & Nanoengineering2023，9，4： | 0 |
| 18 | Click chemistry extracellular vesicle/peptide/chemokine nanocarriers for treating central nervous system injuries显示文摘Central nervous system(CNS)injuries,including stroke,traumatic brain injury,and spinal cord injury,are essential causes of death and long-term disability and are difficult to cure,mainly due to the limited neuron regeneration and the glial scar formation.Herein,we apply extracellular vesicles(EVs)secreted by M2 microglia to improve the differentiation of neural stem cells(NSCs)at the injured site,and simultaneously modify them with the injured vascular targeting peptide(DA7R)and the stem cell recruiting factor(SDF-1)on their surface via copper-free click chemistry to recruit NSCs,inducing their neuronal differentiation,and serving as the nanocarriers at the injured site(Dual-EV).Results prove that the Dual-EV could target human umbilical vascular endothelial cells(HUVECs),recruit NSCs,and promote the neuronal differentiation of NSCs in vitro.Furthermore,10 miRNAs are found to be upregulated in Dual-M2-EVs compared to Dual-M0-EVs via bioinformatic analysis,and further NSC differentiation experiment by flow cytometry reveals that among these miRNAs,miR30b-3p,miR-222-3p,miR-129-5p,and miR-155-5p may exert effect of inducing NSC to differentiate into neurons.In vivo experiments show that Dual-EV nanocarriers achieve improved accumulation in the ischemic area of stroke model mice,potentiate NSCs recruitment,and increase neurogenesis.This work provides new insights for the treatment of neuronal regeneration after CNS injuries as well as endogenous stem cells,and the click chemistry EV/peptide/chemokine and related nanocarriers for improving human health. | Huitong Ruan Yongfang Li Cheng Wang Yixu Jiang Yulong Han Yiwei Li Dandan Zheng Jing Ye Gang Chen Guo-yuan Yang Lianfu Deng Ming Guo Xingcai Zhang Yaohui Tang Wenguo Cui | 2023 | Acta Pharmaceutica Sinica B2023，13，5： | 0 |
| 19 | RSBP15 interacts with and stabilizes dRSPH3 during sperm axoneme assembly in Drosophila显示文摘Flagellum in sperm is composed of over 200 different proteins and is essential for sperm motility. In particular, defects in the assembly of the radial spoke in the flagellum result in male infertility due to loss of sperm motility. However, mechanisms regulating radial spoke assembly remain unclear in metazoans.Here, we identified a novel Drosophila protein radial spoke binding protein 15(RSBP15) which plays an important role in regulating radial spoke assembly. Loss of RSBP15 results in complete lack of mature sperms in seminal vesicles(SVs), asynchronous individualization complex(IC) and defective '9 + 2'structure in flagella. RSBP15 is colocalized with dRSPH3 in sperm flagella, and interacts with dRSPH3 through its DD_R_PKA superfamily domain which is important for the stabilization of dRSPH3. Moreover,loss of dRSPH3, as well as dRSPH1, dRSPH4 a and dRSPH9, showed similar phenotypes to rsbp15 KO mutant. Together, our results suggest that RSBP15 acts in stabilizing the radial spoke protein complex to anchor and strengthen the radial spoke structures in sperm flagella. | Ya Wang Rui Xu Yiwei Cheng Haowei Cao Zibin Wang Tianyu Zhu Jiayin Jiang Hao Zhang Chang Wang Lin Qi Mingxi Liu Xuejiang Guo Juan Huang Jiahao Sha | 2019 | Journal of Genetics and Genomics2019，46，6： | 0 |
| 20 | SiRAP2-12,a Positive Regulatory Factor,Effectively Improves the Waterlogging Tolerance of Foxtail Millet(Setaria italica)显示文摘Foxtail millet(Setaria italica)growth was inhibited because of waterlogging stress,which has caused yield reduc-tion.ERF family plays an important role to plant adversity tolerance.In our study,we obtained 19,819 differential expressed genes(DEGs)between the two treatments based on the RNA-seq sequencing of foxtail millet of water-logging stress.Furthermore,a total of 28 ERF family members were obtained,which have a complete open read-ing frame.We studied the evolution and function of SiERF family and how they affected the waterlogging tolerance.It was found that SiERF1A/B/C(GenBank ID:OR775217,OR775219,OR775218)and SiRAP2-12(GenBank ID:OR775216)have similar functions to the known waterlogging tolerance genes of other plants.Among them,the SiRAP2-12 expression was obviously significantly up-regulated in foxtail millet after 5d water-logging stress.After SiRAP2-12 was silenced,the activity of defense enzymes in millet decreased significantly.In details,superoxide dismutase(SOD),catalase(CAT)and peroxidase(POD),the osmotic regulator proline(Pro),and the activity of the anaerobic respiratory enzyme alcohol dehydrogenase(ADH)content were decreased by 78.61%,29.52%,79.95%,19.41%and 54.77%,respectively.In contrast,the relative electrical conductivity contents(REC),malondialdehyde(MDA),and hydrogen peroxide(H_(2)O_(2))of the foxtail millet subjected to virus-induced gene silencing clearly increased by 1.03-fold,36.09%,and 15.21%,respectively.The content of sodium(Na^(+))in the SiRAP2-12-silenced foxtail millet also increased,but that of potassium(K^(+))decreased.Interestingly,we found that ethylene content was significantly reduced.Further,the SiAOC1 expression,an essential gene for ethylene synthesis,was inhibited in SiRAP2-12-silenced foxtail millet after waterlogging stress.Taken together,we hypothesized that SiRAP2-12 might be a positive regulator of millet tolerance to waterlogging stress. | Xueyan Xia Xiaohong Fu Yu Zhao Jihan Cui Nuoya Xiao Jingxin Wang Yiwei Lu Meihong Huang Cheng Chu Jia Zhang Mengxin Yang Shunguo Li Jianfeng Liu | 2024 | Phyton-International Journal of Experimental Botany2024，93，3： | 0 |
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