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| 1 | A Novel Coronavirus Genome Identified in a Cluster of Pneumonia Cases--Wuhan,China 2019−2020显示文摘Emerging and re-emerging pathogens are great challenges to the public health(1).A cluster of pneumonia cases with an unknown cause occurred in Wuhan starting on December 21,2019.As of January 20,2020,a total of 201 cases of pneumonia in China have been confirmed.A team of professionals from the National Health Commission and China CDC conducted epidemiological and etiological investigations.On January 3,2020,the first complete genome of the novelβgenus coronaviruses(2019-nCoVs)was identified in samples of bronchoalveolar lavage fluid(BALF)from a patient from Wuhan by scientists of the National Institute of Viral Disease Control and Prevention(IVDC)through a combination of Sanger sequencing,Illumina sequencing,and nanopore sequencing.Three distinct strains have been identified,the virus has been designated as 2019-nCoV,and the disease has been subsequently named novel coronavirus-infected pneumonia(NCIP). | Wenjie Tan Xiang Zhao Xuejun Ma Wenling Wang Peihua Niu Wenbo Xu George F.Gao Guizhen Wu | 2020 | China CDC weekly2020，2，4： | 95 |
| 2 | Long non-coding RNAs in cancer显示文摘Long non-coding RNAs (lncRNAs) are a group of RNA transcripts that exceed 200 nt in length, yet lack significant open reading frames (ORFs) [1–4]. In contrast | GONG ZhaoJian ZHANG ShanShan ZHANG WenLing HUANG HongBin LI Qiao DENG Hao MA Jian ZHOU Ming XIANG Juan Juan WU MingHua LI XiaYu XIONG Wei LI XiaoLing LI Yong ZENG ZhaoYang LI GuiYuan | 2012 | Science China(Life Sciences)2012，55，12： | 22 |
| 3 | Nasopharyngeal carcinoma:Advances in genomics and molecular genetics显示文摘Nasopharyngeal carcinoma (NPC) is a squamous-cell carcinoma that arises in the epithelial lining of the nasopharynx [1]. This neoplasm has a notable ethnic and geographic distribution,being of high prevalence in southern China but rare in other parts of the world [2]. | ZENG ZhaoYang HUANG HongBin ZHANG WenLing XIANC Bo ZHOU Ming ZHOU YanHong MA Jian YI Mei LI XiaYu LI XiaoLing XIONG Wei LI GuiYuan | 2011 | Science China(Life Sciences)2011，54，10： | 13 |
| 4 | FAM19A4 is a novel cytokine ligand of formyl peptide receptor I (FPR 1) and is able to promote the migration and phagocytosis of macrophages显示文摘FAM19A4 是为有顺序类似 19 的家庭的缩写(chemokine (C-C 主题)-like) 成员 A4,它能分泌的蛋白质在正常纸巾在底层被表示。坚定的 FAM19A4 遗体的生物功能,和它的潜在的受体是 unclarified。在这研究,我们证明 FAM19A4 是古典能分泌的蛋白质,我们第一次证实它的成熟蛋白质由 95 氨基酸组成。我们发现这新奇 cytokine 的表示是 upregulated 在里面 lipopolysaccharide (LPS ) 刺激了单核白血球和巨噬细胞并且典型地在极化的 M1。FAM19A4 在巨噬细胞上显示出趋化性的活动并且随蛋白质 kinase B (Akt ) 的 phosphorylation 的显著增加在 vitro 并且在 vivo 提高 zymosan 的巨噬细胞吞噬作用。FAM19A4 能也在 zymosan 刺激之上增加反应的氧种类(ROS ) 的版本。基于受体成为主观,收音机 ligand 绑定试金和受体阻塞,而且,我们第一次证明 FAM19A4 是甲酰肽受体 1 的新奇 ligand (FPR1 ) 。上述数据显示在煽动性的刺激之上, monocyte/macrophage-derived FAM19A4 可以在病原的感染期间在巨噬细胞的移植和激活起一个关键作用。 | Wenyan Wang Ting Li Xiaolin Wang Wanxiong Yuan Yingying Cheng Heyu Zhang Enquan Xu Yingmei Zhang Shuang Shi Dalong Ma Wenling Han | 2015 | Cellular & Molecular Immunology2015，12，5： | 8 |
| 5 | Effect of suppressing dust by multi-direction whirling air curtain on fully mechanized mining face显示文摘A combined method of numerical simulation and field testing was adopted in this study in the interest of solving the problem of hard to control high concentrate dusts on a fully mechanized mining face.In addition,the dust suppression effect of a multi-direction whirling air curtain was studied in this paper.Under the influence of the wall attachment effect,the compressed air which blows out from the two-phase or three-phase radial outlets on the generator of the air curtain can form a multi-direction whirling air curtain,which can cover the whole roadway section of a fully mechanized mining face.The traditional method of controlling dust is a forcing system with exhaust overlap which has the major disadvantage of lacking a jet effect and consequently results in poor dust control.It is difficult to form the air flow field within the range of L_p5Sr^(1/2).However,due to the effect of this novel system,the radial airflow can be turned into axial airflow allowing fresh air to flow through the length of the heading.The air flow field which is good at controlling dust diffusion can be formed 12.8 m from the heading face.Furthermore,the field measurement results show that before the application of a multi-direction whirling air curtain,the dust concentration is 348.6 mg/m^3 and 271.4 mg/m^3 respectively at the roadway cross-section measurement points which are 5 m and 10 m from the heading face.However,after the application of the multi-direction whirling air curtain,the dust concentration is only 61.2 mg/m^3 and 14.8 mg/m^3,respectively.Therefore,the dust control effect of a multi-direction whirling air curtain is obvious. | Nie Wen Liu Yanghao Wei Wenle Hu Xiangming Ma Xiao Peng Huitian | 2016 | International Journal of Mining Science and Technology2016，26，4： | 6 |
| 6 | CMTM4 inhibits cell proliferation and migration via AKT, ERK1/2,and STAT3 pathway in colorectal cancer显示文摘CMTM4(CKLF-like MARVEL transmembrane domain containing 4),a potential tumor suppressor gene,is involved in several types of malignancies.It has been reported to be downregulated and exhibit anti-tumorigenic activities by regulating cell growth and cell cycle in clear cell renal cell carcinoma.It has also been identified as a tumor suppressor in hepatocellular carcinoma(HCC),and its negative expression is a risk factor for poor prognosis of HCC patients.In the present study,an integrated bioinformatics analysis based on The Cancer Genome Atlas(TCGA)database showed that CMTM4 was frequently reduced in colorectal cancer(CRC)and high expression of CMTM4 was associated with increased overall survival rates.Based on these findings,we adopted gain-of-function and lost-of-function strategies using SW480 and HT29 CRC cell lines which have relatively low and high endogenous CMTM4 levels,respectively.We observed impeded cell proliferation and migration upon overexpression of CMTM4 in SW480 cells,and the opposite effects were observed upon knockdown of CMTM4 in HT-29 cells.Cell signaling pathways essential for CRC progression were then examined,and the phosphorylation levels of AKT,ERK1/2,and STAT3 were found to be decreased by CMTM4 overexpression in SW480 cells and elevated by CMTM4 silencing in HT29 cells.Their inhibitors were used to validate that the three signaling pathways contributed to the inhibitory effects of CMTM4 on CRC cells.Taken together,our results suggest that CMTM4 plays a tumor suppressive role in CRC. | Hui Xue Ting Li Pingzhang Wang Xiaoning Mo Hejun Zhang Shigang Ding Dalong Ma Wenping Lv Jing Zhang Wenling Han | 2019 | Acta Biochimica et Biophysica Sinica2019，51，9： | 5 |
| 7 | Design and application of 60mer oligonucleotide microarray in SARS coronavirus detection显示文摘The 60mer oligonucleotide microarray was designed and applied to detecting of SARS (severe acute res-piratory syndrome) coronavirus. Thirty 60mer specific oligos were designed to cover the whole genome of the first submit-ted coronavirus strain, according to the sequence of TOR2 (GENEBANK Accession: AY274119). These primers were synthesized and printed into a microarray with 12×12 spots. RNAs were extracted from the throat swab and gargling fluid of SARS patients and reverse-transcripted into the double strand cDNAs. The cDNAs were prepared as re-stricted cDNA fragments by the restriction display (RD) technique and labeled by PCR with the Cy5-universal primer. The labeled samples were then applied to the oligo microar-ray for hybridization. The diagnostic capability of the mi-croarray was evaluated after the washing and scanning steps. The scanning result showed that samples of SARS patients were hybridized with multiple SARS probes on the microar-ray, and there is no signal on the negative and blank controls. These results indicate that the genome of SARS coronavirus can be detected in parallel by the 60mer oligonucleotide mi-croarray, which can improve the positive ratio of the diagno-sis. The oligo microarray can also be used for monitoring the behavior of the virus genes in different stages of the disease status. | SHI Rong MA Wenli WU Qinghua ZHANG Bao SONG Yanbin GUO Qiuye XIAO Weiwei WANG Yan ZHENG Wenling | 2003 | Chinese Science Bulletin2003，48，12： | 4 |
| 8 | A core-shell structured COYID-19 mRNA vaccine with favorable biodistribution pattern and promising immunity显示文摘Although inoculation of COVID-19 vaccines has rolled out globally,there is still a critical need for safe and effective vaccines to ensure fair and equitable supply for all countries.Here,we report on the development of a highly efficacious mRNA vaccine,SW0123 that is composed of sequence-modified mRNA encoding the full-length SARS-CoV-2 Spike protein packaged in core-shell structured lipopolyplex(LPP)nanoparticles.SWOT 23 is easy to produce using a large-scale microfluidics-based apparatus.The unique core-shell structured nanoparticle facilitates vaccine uptake and demonstrates a high colloidal stability,and a desirable biodistribution pattern with low liver targeting effect upon intramuscular administration.Extensive evaluations in mice and nonhuman primates revealed strong immunogenicity of SW0123,represented by induction of Th1-polarized T cell responses and high levels of antibodies that were capable of neutralizing not only the wild-type SARS-CoV-2,but also a panel of variants including D614G and N501Y variants.In addition,SW0123 conferred effective protection in both mice and non-human primates upon SARS-CoV-2 challenge.Taken together,SW0123 is a promising vaccine candidate that holds prospects for further evaluation in humans. | Ren Yang Yao Deng Baoying Huang Lei Huang Ang Lin Yuhua Li Wenling Wang Jingjing Liu Shuaiyao Lu Zhenzhen Zhan Yufei Wang Ruhan A Wen Wang Peihua Niu Li Zhao Shiqiang Li Xiaopin Ma Luyao Zhang Yujian Zhang Weiguo Yao Xingjie Liang Jincun Zhao Zhongmin Liu Xiaozhong Peng Hangwen Li Wenjie Tan | 2021 | Signal Transduction and Targeted Therapy2021，6，6： | 3 |
| 9 | Excellent Terahertz shielding performance of ultrathin flexible Cu/graphene nanolayered composites with high stability显示文摘Electromagnetic interference(EMI)shielding at Terahertz(THz)frequency range attracts increasing attention due to the rapid development of THz science and technologies.EMI shielding materials with small thickness,high shielding effectiveness(SE),good flexibility and stability are highly desirable.Herein,an ultrathin flexible copper/graphene(Cu/Gr)nanolayered composite are prepared,which can reach the average EMI SE of 60.95 dB at 0.1–1.0 THz with a thickness of only 160 nm,indicating that more than 99.9999%of the THz wave power can be shielded.Furthermore,the Cu/Gr nanolayered composite also exhibits excellent oxidation resistance,with a 93.09%maintenance rate for EMI SE value after heating at 120℃for 3 h in air,far higher than that of the bare Cu film(62.15%).Besides,the Cu/Gr nanolayered composite exhibits good mechanical flexibility and flexural fatigue resistance.The EMI SE value of the Cu/Gr nanolayered composite shows a maintenance rate of 98.87%even after 1500 times bending cycles,obviously higher than that of multilayer Cu film(93.07%).These results demonstrate that the ultrathin flexible Cu/Gr nanolayered composites with excellent shielding performance and good stability have a broad application prospect in THz shielding for wearable devices and next generation mobile communication equipment. | Shengyue Hou Wenle Ma Guanghao Li Yi Zhang Yunyun Ji Fei Fan Yi Huang | 2020 | Journal of Materials Science & Technology2020，52，17： | 2 |
| 10 | Molecular cloning and characterization of chemokine-like factor 1 ( CKLF1 ), a novel human cytokine with unique structure and potential chemotactic activity显示文摘 | Wenling HAN Yaxin LOU Dalong MA | 2001 | Biochem J2001，357，1： | 1 |
| 11 | Secretory expression of the fusion protein composed of human IL-2 and PreS antigen of hepatitis B virus in mammalian cells显示文摘In order to make entire HBV preSAg secrete from mammalian cells, we constructed an eukaryotic expression vector by using leader sequence of human interleukin-2 (IL-2) as secretory signal peptide, and using high hydrophilic amino acids as the linker between IL-2 C end and preSAg N end. As a result, the IL-2preS fusing protein could be secreted from mammalian cells transfected with the reconstructed vector and the expression efficiency was identical to that of natural IL-2. It was considered that the retentive effect of preS1Ag could be successfully bypassed. The results not only laid a theoretical and practical foundation for constructing specific gene vaccine against HBV persistent infection, but also supplied experimental evidence for studying modulation of protein secretory expression. | Zhangguo Chen Dalong Ma Jing Wang Yingmei Zhang Yong Yuan Wenling Han Hongtao Liu | 1998 | Chinese Science Bulletin1998，43，9： | 1 |
| 12 | CHD5, a tumor suppressor that is epigenetically silenced in lung cancer显示文摘 | Rui Zhao Qitao Yan Jingye Lv Haili Huang Wenling Zheng Bao Zhang Wenli Ma | 2011 | Lung Cancer2011，，： | 1 |
| 13 | Macroanatory of coronary arteries in Bactrian camel (Camelus bactrianus) 显示文摘 | Guoqiang Yuan Jinghong Ma Wenling Ye etc | 2009 | Vet Res Commun2009，，33： | 1 |
| 14 | Insight of unresolved complex mixtures of saturated hydrocarbons in heavy oil via GC×GC-TOFMS analysis显示文摘The saturated hydrocarbon fraction of a heavily biodegraded crude oil from Liaohe oilfield was analyzed by using comprehensive two-dimensional gas chromatography coupled with time-of-flight mass spectrometry (GC×GC-TOFMS). The 'humps', which was termed as unresolved complex mixtures (UCMs) in conventional gas chromatographic analysis, was separated for identification of the individual compound. Main compounds of UCMs were identified according to the combined information of characteristics of GC×GC chromatogram and time-of-flight mass spectra. It's found that UCMs are mainly composed of great number of ring compounds with extremely low concentration. The ring compounds are separated according to their boiling point diversity in the first dimension column, then to their polarity or ring number variety in the second dimension column. In the sample there are two groups of UCMs, i.e., the first group compounds with boiling point lower that of C24 and the second group compounds with boiling point higher than that of C24. The first group of UCMs mainly consists of monocyclic, bicyclic and tricyclic ring compounds. The first group of UCMs is mainly composed of saturated hydrocarbons, in which the percentage of ring compounds with signal-to-noise ratio above 100 is about 75% of the total quantity of saturated hydrocarbon, while its mass is over 80% of the total saturated hydrocarbon. The second group of UCMs is mainly composed of tetracyclic and pentacyclic ring compounds, in which the percentage of UCM compounds with signal-to-noise ratio over 100 is about 17% of the total quantity of saturated hydrocarbon, and its mass is about 0.5% of the total saturated hydrocarbon. The results of this study are helpful for heavy oil developing and genesis mechanism understanding. | WANG HuiTong ZHANG ShuiChang WENG Na WEI XiaoFang ZHU GuangYou YU Han BI LiNa MA WenLing | 2013 | Science China Chemistry2013，56，2： | 0 |
| 15 | ACSL5,a prognostic factor in acute myeloid leukemia,modulates the activity of Wnt/β-catenin signaling by palmitoylation modification显示文摘Acyl-CoA synthetase long chain family member 5(ACSL5),is a member of the acyl-CoA synthetases(ACSs)family that activates long chain fatty acids by catalyzing the synthesis of fatty acyl-CoAs.The dysregulation of ACSL5 has been reported in some cancers,such as glioma and colon cancers.However,little is known about the role of ACSL5 in acute myeloid leukemia(AML).We found that the expression of ACSL5 was higher in bone marrow cells from AML patients compared with that from healthy donors.ACSL5 level could serve as an independent prognostic predictor of the overall survival of AML patients.In AML cells,the ACSL5 knockdown inhibited cell growth both in vitro and in vivo.Mechanistically,the knockdown of ACSL5 suppressed the activation of the Wnt/β-catenin pathway by suppressing the palmitoylation modification of Wnt3a.Additionally,triacsin c,a pan-ACS family inhibitor,inhibited cell growth and robustly induced cell apoptosis when combined with ABT-199,the FDA approved BCL-2 inhibitor for AML therapy.Our results indicate that ACSL5 is a potential prognosis marker for AML and a promising pharmacological target for the treatment of molecularly stratified AML. | Wenle Ye Jinghan Wang Jiansong Huang Xiao He Zhixin Ma Xia Li Xin Huang Fenglin Li Shujuan Huang Jiajia Pan Jingrui Jin Qing Ling Yungui Wang Yongping Yu Jie Sun Jie Jin | 2023 | Frontiers of Medicine2023，17，4： | 0 |
| 16 | Genomic Recombination of SARS-CoV-2 Subvariants BA.5.2.48 and BF.7.14—China,2023显示文摘Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),a single-stranded positive RNA virus,exhibits a high rate of genome mutation and recombination(1–2).Recombinant strains of this virus could potentially affect their binding ability to the ACE2 receptor,which may in turn diminish the protection offered by vaccines and neutralizing antibodies.Notably,the Delta and Omicron recombinations have been collectively referred to as Deltacron(3).Furthermore,XBB.1.5,a recombinant strain resulting from the combination of the BA.2 sublineages BA.2.10.1 and BA.2.75,has demonstrated enhanced transmissibility(4).As such,it is imperative to monitor the genome recombination of SARS-CoV-2 in order to provide valuable insights regarding epidemic and transmission trends. | Zhongxian Zhang Han Li Hongxia Ma Wenling Wang Dawei Gao Fei Ye Weibang Huo Yuda Chen Changcheng Wu Wenjie Tan | 2023 | China CDC weekly2023，5，14： | 0 |
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