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| 1 | One-step generation of p53 gene biallelic mutant Cynomolgus monkey via the CRISPR/Cas system显示文摘 | Haifeng Wan Chunjing Feng Fei Teng Shihua Yang Baoyang Hu Yuyu Niu Andy Peng Xiang Weizhen Fang Weizhi Ji Wei Li Xiaoyang Zhao Qi Zhou | 2015 | Cell Research2015，25，2： | 39 |
| 2 | CD4^(+) T cell-released exosomes inhibit CD8^(+) cytotoxic T-lymphocyte responses and antitumor immunity显示文摘T cells secrete bioactive exosomes(EXO),but the potential immunoregulatory effect of T-cell EXO is largely unknown.In this study,we generated activated ovalbumin(OVA)-specific CD4^(+) T cells in vitro via coculture of OVA-pulsed dendritic cells(DC_(OVA))with naive CD4^(+) T cells derived from OVA-specific T-cell receptor(TCR)transgenic OTII mice.CD4^(+) T-cell EXO were then purified from the CD4^(+) T-cell culture supernatants by differential ultracentrifugation.CD4^(+) T-cell EXO exhibited the‘saucer’shape that is characteristic of EXO with a diameter between 50 and 100 nm,as assessed by electron microscopy,and contained the EXO-associated proteins LAMP-1,TCR and lymphocyte function associated antigen-1(LFA-1),as determined by western blot.Flow cytometric analysis showed that CD4^(+) T-cell EXO expressed CD4^(+) T-cell markers(CD4,TCR,LFA-1,CD25 and Fas ligand),but to a lesser extent than CD4^(+) T cells.We demonstrated that DC_(OVA) took up CD4^(+) T-cell EXO via peptide/major histocompatibility complex(pMHC)II/TCR and CD54/LFA-1 interactions.OVA-specific CD4^(+) T-cell EXO from OTII mice,but not ConA-stimulated polyclonal CD4^(+) T-cell EXO from wild-type C57BL/6 mice inhibited DC_(OVA)-stimulated in vitro CD4^(+) T-cell proliferation and in vivo CD81 cytotoxic T lymphocyte(CTL)responses and antitumor immunity against OVA-expressing B16 melanoma BL6-10OVA cells.In addition,EXO derived from a T-cell hybridoma cell line,MF72.2D9,expressing an OVA-specific CD4^(+) TCR,had a similar inhibitory effect as OTII CD4^(+) T-cell EXO on CTL-mediated antitumor immunity.Taken together,our data indicate that antigen-specific T-cell EXO may serve as a new type of immunosuppressive reagent for use in transplant rejection and treatment of autoimmune diseases. | Haifeng Zhang Yufeng Xie Wei Li Rajni Chibbar Sidong Xiong Jim Xiang | 2011 | Cellular & Molecular Immunology2011，8，1： | 10 |
| 3 | Early Cretaceous uplift history of the Dabie orogenic belt: Evidence from pluton emplacement depths显示文摘The North Dabie complex unit(NDC)is a widely exposed petro-tectonic unit.To understand post-orogenic uplift history and mechanism of the Dabie orogenic belt,many samples of granite,granodiorite,diorite,and alkali feldspar granite with precise zircon U-Pb ages were collected for electron microprobe analysis of hornblende.Emplacement pressure values were then calculated by using an Al-in-hornblende geobarometer.The result shows that total Al(AlT)values in hornblende range from 1.17to 1.97 p.f.u.,XFe(=Fe/(Fe+Mg))from 0.4 to 0.65,and corresponding pressure values from 256.8 to 676.2 MPa for AlT greater than 1.17.It is suggested that although the main mechanism for the uplift is tectonic extension,inhomogeneous uplift related to the pluton emplacement is also important during post-orogenic evolution of the Dabie orogenic belt.Emplacement depth distribution of the plutons shows that uplift in the NDC is affected by horst-graben structures since 120 Ma. | WANG YongSheng WANG HaiFeng SHENG Yong XIANG BiWei | 2014 | Science China Earth Sciences2014，57，5： | 8 |
| 4 | Mammalian cell display for rapid screening scFv antibody therapy显示文摘人的抗体开始在有免疫力的基因治疗为使用正在引起注意。对癌症和传染疾病的治疗合适的有效治疗学的 monoclonal 抗体的有效产生将是极其珍贵的。抗体显示方法逐渐地被用来屏蔽人的 monoclonal 抗体。这里,我们报导一个哺乳动物的房间显示方法的构造源于一个天真的抗体全部剧目,为人,单个链的变量碎裂短暂地基于 pDisplay 向量在 293T 房间表面上被显示了。当前的 pDisplayscFv 抗体全部剧目的尺寸被估计了是 0. | Jing Zhang Xiao'ai Zhang Qiang Liu Mengyi Li Liucun Gao Xin Gao Shensi Xiang Liangliang Wu Jie Fu Haifeng Song | 2014 | Acta Biochimica et Biophysica Sinica2014，46，10： | 6 |
| 5 | Visual Localization in Urban Area Using Orthogonal Building Boundaries and a GIS Database显示文摘A framework is presented for robustly estimating the location of a mobile robot in urban areas based on images extracted from a monocular onboard camera, given a 2D map with building outlines with neither 3D geometric information nor appearance data. The proposed method firstly reconstructs a set of vertical planes by sampling and clustering vertical lines from the image with random sample consensus (RANSAC), using the derived 1D homographies to inform the planar model. Then, an optimal autonomous localization algorithm based on the 2D building boundary map is proposed. The physical experiments are carried out to validate the robustness and accuracy of our localization approach. | LI Haifeng LIU Jingtai LU Xiang | 2012 | 机器人2012，34，5： | 5 |
| 6 | In vivo degradation and endothelialization of an iron bioresorbable scaffold显示文摘Detection of in vivo biodegradation is critical for development of next-generation medical devices such as bioresorbable stents or scaffolds(BRSs).In particular,it is urgent to establish a nondestructive approach to examine in vivo degradation of a new-generation coronary stent for interventional treatment based on mammal experiments;otherwise it is not available to semi-quantitatively monitor biodegradation in any clinical trial.Herein,we put forward a semi-quantitative approach to measure degradation of a sirolimus-eluting iron bioresorbable scaffold(IBS)based on optical coherence tomography(OCT)images;this approach was confirmed to be consistent with the present weight-loss measurements,which is,however,a destructive approach.The IBS was fabricated by a metal-polymer composite technique with a polylactide coating on an iron stent.The efficacy as a coronary stent of this new bioresorbable scaffold was compared with that of a permanent metal stent with the name of trade mark Xience,which has been widely used in clinic.The endothelial coverage on IBS was found to be greater than on Xience after implantation in a rabbit model;and our well-designed ultrathin stent exhibited less individual variation.We further examined degradation of the IBSs in both minipig coronary artery and rabbit abdominal aorta models.The present result indicated much faster iron degradation of IBS in the rabbit model than in the porcine model.The semi-quantitative approach to detect biodegradation of IBS and the finding of the species difference might be stimulating for fundamental investigation of biodegradable implants and clinical translation of the next-generation coronary stents. | Wenjiao Lin Hongjie Zhang Wanqian Zhang Haiping Qi Gui Zhang Jie Qian Xin Li Li Qin Haifeng Li Xiang Wang Hong Qiu Xiaoli Shi Wei Zheng Deyuan Zhang Runlin Gao Jiandong Ding | 2021 | Bioactive Materials2021，6，4： | 3 |
| 7 | Structural insights into a dual-specificity histone demethylase ceKDM7A from Caenorhabditis elegans显示文摘Histone 离氨酸 methylation 能被 JmjC 包含域的蛋白质在 sequence-state-specific 和 methylation-state-specific 举止移开。然而,底层特性怎么样,决定,酶怎么被调整,大部分是未知的。我们最近发现了那 ceKDM7A,一个哲学博士 -- 并且 JmjC 包含域的蛋白质,是为 H3K9me2 特定的 histone demethylase, H3K27me2,和 PHD 摸绑定到 H3K4me3 指南在 vivo 的 demethylation 活动。为了为酶的活动和 PHD 的功能提供结构的卓见进分子的机制,摸,我们与包含 H3K4me3, H3K9me2,和 H3K27me2 修正的各种各样的联合的单身者或二肽一起在 apo 形式并且在建筑群解决了酶的六水晶结构。结构显示 H3K9me2 和 H3K27me2 以一种类似的方式与 ceKDM7A 交往,并且肽绑定特性被特定的相互作用的一个网络决定。结构的几何测量也揭示了与 PHD 手指和 H3K9me2 界限联系到 JmjC 领域的那 H3K4me3 从二个分开的分子,建议 trans-histone 肽绑定机制。因此,我们的全身的结构的研究重要地由催化领域而且更多揭示底层识别不仅,为 H3K9me2 和 H3K27me2 的 ceDKM7A 的双特性的分子的机制。 | Ying Yang Lulu Hu Ping Wang Haifeng Hou Yan Lin Yi Liu Ze Li Rui Gong Xiang Feng Lu Zhou Wen Zhang Yuhui Dong Huirong Yang Hanqing Lin Yiqin Wang Charlie Degui Chen Yanhui Xu | 2010 | Cell Research2010，20，8： | 3 |
| 8 | Atomically precise metal-chalcogenide semiconductor molecular nanoclusters with high dispersibility:Designed synthesis and intracluster photocarrier dynamics显示文摘A comprehensive understanding of excited-state dynamics of semiconductor quantum dots or nanomaterials at the atomic or molecular level is of scientific importance.Pure inorganic(or non-covalently protected)seimiconductor molecular nanoclusters with atomically precise structure are contributive to establish accurate correlation of excited-state dynamics with their composition/structure,however,the related studies are almost blank because of unresolved solvent dispersion issue.Herein,we designedly created the largest discrete chalcogenide seimiconductor molecular nanoclusters(denoted P2-CuMSnS,M=In or/and Ga)with great dispersibility,and revealed an interesting intracluster“core–shell”charge transfer relaxation dynamics.A systematic red shift in absorption spectra with the gradual substitution of In by Ga was experimentally and computationally investigated,and femtosecond transient absorption measurements further manifested there were three ultrafast processes in excited-state dynamics of P2 nanoclusters with the corresponding amplitudes directed by composition variation.Current results hold the great promise of the solution-processible applications of semiconductor-NC-based quantum dots and facilitate the development of atomically precise nano-chemistry. | Jiaxu Zhang Chaochao Qin Yeshuang Zhong Xiang Wang Wei Wang Dandan Hu Xiaoshuang Liu Chaozhuang Xue Rui Zhou Lei Shen Yinglin Song Dingguo Xu Zhien Lin Jun Guo Haifeng Su Dong-Sheng Li Tao Wu | 2020 | Nano Research2020，13，10： | 2 |
| 9 | Duodenal-jejunal bypass surgery on type 2 diabetic rats reduces the expression of matrix metalloproteinase-9 and tissue inhibitor of matrix metalloproteinase-1 in the thoracic aorta显示文摘 | Maimaitiyusufu Wubulikasimu Han Haifeng Yan Zhibo Zhang Xiang Liu Shaozhuang Zhang Guangyong Kasimu Aimaiti Hu Sanyuan | 2014 | Chinese Medical Journal2014，，13： | 2 |
| 10 | Petrogenesis and Tectonic Significance of the Three-Period Porphyries from the Daruoluolong Cu(Au) Deposit, Tibet, China显示文摘The Daruoluolong deposit is the first high-grade Cu(Au) deposit discovered in the middle section of theBangonghu-Nujiang (herein after referred to as Ban-Nu)metallogenic belt, which has not been documented aboutpreviously. This deposit is located in Shuanghu County ofnorthern Tibet, and its geotectonic position belongs to thenorthern margin of the Ban-Nu suture and southern marginof the Qiangtang terrane. There are three-periodporphyries including ore-bearing granodiorite porphyry,barren granite porphyry and biotite granodiorite porphyryin the deposit. In this paper, we present new zircon U-Pbdate, petrogeochemistry, and Hf isotopic compositions toassess host rock and mineralization age, rock type andmagma source. We have also discussed the metallogenicbackground of the Daruoluolong deposit which isconsistent with that of Naruo the deposit in the westernsection of the Ban-Nu metallogenic belt (Ding Shuai et al.,2017). | GAO Ke SONG Yang LIU Zhibo FANG Xiang LI Faqiao LI Haifeng | 2018 | Acta Geologica Sinica(English Edition)2018，92，3： | 2 |
| 11 | An in vivo Transient Expression System Can Be Applied for Rapid and Effective Selection of Artificial MicroRNA Constructs for Plant Stable Genetic Transformation显示文摘The utility of artificial microRNAs(amiRNAs) to induce loss of gene function has been reported for many plant species,but expression efficiency of the different amiRNA constructs in different transgenic plants was less predictable,In this study,expressions of amiRNAs through the gene backbone of Arabidopsis miR168a were examined by both Agrobacterium-mediated transient expression and stable plant genetic transformation.A corresponding trend in expression of amiRNAs by the same amiRNA constructs between the transient and the stable expression systems was observed in the experiments.Plant genetic transformation of the constructs that were highly expressible in amiRNAs in the transient agro-infiltration assays resulted in generation of transgenic lines with high level of amiRNAs.This provides a simple method for rapid and effective selection of amiRNA constructs used for a time-consuming genetic transformation in plants. | Basdeo Bhagwat Ming Chi Li Su Haifeng Tang Guiliang Tang Yu Xiang | 2013 | Journal of Genetics and Genomics2013，40，5： | 1 |
| 12 | Fano-resonance-based mach-zehnder optical switch employing dual-bus coupled ring resonator as two-beam interferometer显示文摘 | Fan Wang Xiang Wang Haifeng Zhou | 2009 | Optics Express2009，17，9： | 1 |
| 13 | Enzymatic hydrolysis of wheat gluten by proteases and properties of the resulting hydrolysates显示文摘 | Xiang zhen Kong Huiming Zhou Haifeng Qian | 2007 | Food Chemistry2007，102，： | 1 |
| 14 | MiR-495 functions as an adjuvant to radiation therapy by reducing the radiation-induced bystander effect显示文摘 | Jie Fu Mengmeng Jiang Meng Zhang Jing Zhang Yu Wang Shensi Xiang Xiaojie Xu Qinong Ye Haifeng Song | 2016 | Acta Biochimica et Biophysica Sinica2016，48，11： | 1 |
| 15 | Effect of manufacturing parameters on the release profiles of casein-loaded alginate micro- spheres prepared by emulsification/internal gelation 显示文摘 | LI RANRAN ZHANG XING XIANG SHI HAIFENG | 2011 | Journal of Controlled Release2011，152，1： | 1 |
| 16 | Disassembly AND/OR Graph Model for“Disassembly for Recycling”显示文摘 | Gao Jiangang Xiang Dong Chen Haifeng | 2003 | Electronics and the Environment2003，，： | 1 |
| 17 | Direct laser writing breaking diffraction barrier based on two-focus parallel peripheralphotoinhibition lithography显示文摘Direct laser writing(DLW)enables arbitrary three-dimensional nanofabrication.However,the diffraction limit poses a major obstacle for realizing nanometer-scale features.Furthermore,it is challenging to improve the fabrication efficiency using the currently prevalent single-focal-spot systems,which cannot perform high-throughput lithography.To overcome these challenges,a parallel peripheral-photoinhibition lithography system with a sub-40-nm two-dimensional feature size and a sub-20-nm suspended line width was developed in our study,based on two-photon polymerization DLW.The lithography efficiency of the developed system is twice that of conventional systems for both uniform and complex structures.The proposed system facilitates the realization of portable DLW with a higher resolution and throughput. | Dazhao Zhu Liang Xu Chenliang Ding Zhenyao Yang Yiwei Qiu Chun Cao Hongyang He Jiawei Chen Mengbo Tang Lanxin Zhan Xiaoyi Zhang Qiuyuan Sun Chengpeng Ma Zhen Wei Wenjie Liu Xiang Fu Cuifang Kuang Haifeng Li Xu Liu | 2022 | Advanced Photonics2022，4，6： | 1 |
| 18 | An SIRS epdemic model with pulse vaccination and non-monotonic in- cidence rate 显示文摘 | ZHANG Xiaobing HUO Haifeng XIANG Hong | 2013 | Nonlinear Analysis : Hybrid Systems2013，8，5： | 1 |
| 19 | Near-infrared phosphorrescence: Materials and applications 显示文摘 | XIANG Haifeng CHENG Jinghui MA Xiaofeng | 2013 | Chem Soc Rev2013，42，14： | 1 |
| 20 | Bifurcation and stability analysis in predator prey model with a stage-structure for predator显示文摘 | Sun Xiaoke Huo Haifeng Xiang Hong | 2009 | Nonlinear Dyn2009，58，3： | 1 |
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