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| 1 | Diver CE versus Guardwire Plus for thrombectomy in patients with inferior myocardial infarction:a trial of aspiration of thrombus during primary angioplasty for inferior myocardial infarction显示文摘背景不同可行、安全的 thrombectomy 和远侧的保护设备用 Diver CE 设备在临床的 practice.The 效率和附属物 thrombectomy 的安全被使用了( Invatec ,意大利 )对 Guardwire 正设备( Medtronic ,美国)在经皮的冠的干预(一种总线标准)与尖锐劣等的 ST-segment-elevation 心肌的梗塞( STEMI )在病人被作比较不到 12 个小时以前,在心肌的梗塞( TIMI )的 thrombolysis 流动分级 0~1 ,并且总计吸藏第二等的结束点被让结束心脏舒张的体积( LVEDV )室,左室喷射部分( LVEF )和包括死亡的主要不利心脏的事件(权标),心肌的梗塞,在 122 个病人的 30 days.Results A 总数的目标容器 revascularization 和击同等地被划分成 Diver CE 组和 Guardwire 正组,它到年龄是可比较的(( 60 ?????????汣灯摩杯敲????朠潲灵?潃据畬楳湯??湡獵? | YAN Hong-bing WANG Jian LI Nan ZHU Xiao-ling GAO Hai AI Hui LI Xiang YE Ming CHI Yun-peng ZHANG Hong | 2007 | Chinese Medical Journal2007，，7： | 29 |
| 2 | A selective tropism of transfused oval cells for liver显示文摘AIM: To explore the biological behaviors of hepatic oval cells after transfused into the circulation of experimental animals.METHODS: Oval cells from male SD rat were transfused into the circulation of a female rat which were treated by a 2-AAF/CCl4 program, through caudal vein. Sex-determining gene sry which located on Y chromosome was examined by PCR and in situ hybridization technique in liver, kidney and spleen of the experimental animals, respectively.RESULTS: The results of the cell-transplant experiment showed that the srygene was detectable only in the liver but not in spleen and kidney of the experimental rats, and no signals could be detected in the control animals. It can be also morphologically proved that some exogenous cells had migrated into the parenchyma of the liver and settled there.CONCLUSION: The result means that there are exogenous cells located in the liver of the experimental animal and the localization is specific to the liver. This indicates that some 'signal molecules' must exist in the circulation of the rats treated by 2-AAF/CCl4. These 'signal molecules' might play an important role in specific localization and differentiation of transfused oval cells. | Jian-Zhi Chen Hai Hong Jin Xiang Ling Xue Guo-Qiang Zhao Department of Pathology, Medical School of Sun Yat-Sen University, Guangzhou 510080, Guangdong Province, China | 2003 | World Journal of Gastroenterology2003，9，3： | 25 |
| 3 | Laser performance of the SG-Ⅲ laser facility显示文摘SG-Ⅲ laser facility is now the largest laser driver for inertial confinement fusion research in China. The whole laser facility can deliver 180 kJ energy and 60 TW power ultraviolet laser onto target, with power balance better than 10%.We review the laser system and introduce the SG-Ⅲ laser performance here. | Wanguo Zheng Xiaofeng Wei Qihua Zhu Feng Jing Dongxia Hu Jingqin Su Kuixing Zheng Xiaodong Yuan Hai Zhou Wanjun Dai Wei Zhou Fang Wang Dangpeng Xu Xudong Xie Bin Feng Zhitao Peng Liangfu Guo Yuanbin Chen Xiongjun Zhang Lanqin Liu Donghui Lin Zhao Dang Yong Xiang Xuewei Deng | 2016 | High Power Laser Science and Engineering2016，4，3： | 13 |
| 4 | Autophagy Accompanied with Bisdemethoxycurcumin-induced Apoptosis in Non-small Cell Lung Cancer Cells显示文摘Objective To investigate the effects of bisdemethoxycurcumin(BDMC) on non-small cell lung cancer(NSCLC) cell line, A549, and the highly metastatic lung cancer 95 D cells. Methods CCK-8 assay was used to assess the effect of BDMC on cytotoxicity. Flow cytometry was used to evaluate apoptosis. Western blot analysis, electron microscopy, and quantification of GFP-LC3 punctuates were used to test the effect of BDMC on autophagy and apoptosis of lung cancer cells. Results BDMC inhibited the viability of NSCLC cells, but had no cytotoxic effects on lung small airway epithelial cells(SAECs). The apoptotic cell death induced by BDMC was accompanied with the induction of autophagy in NSCLC cells. Blockage of autophagy by the autophagy inhibitor 3-methyladenine(3-MA) repressed the growth inhibitory effects and induction of apoptosis by BDMC. In addition, BDMC treatment significantly decreased smoothened(SMO) and the transcription factor glioma-associated oncogene 1(Gli1) expression. Furthermore, depletion of Gli1 by si RNA and cyclopamine(a specific SMO inhibitor) induced autophagy. Conclusion Aberrant activation of Hedgehog(Hh) signaling has been implicated in several human cancers, including lung cancers. The present findings provide direct evidence that BDMC-induced autophagy plays a pro-death role in NSCLC, in part, by inhibiting Hedgehog signaling. | XU Jin Hong YANG He Ping ZHOU Xiang Dong WANG Hai Jing GONG Liang TANG Chun Lan | 2015 | Biomedical and Environmental Sciences2015，28，2： | 9 |
| 5 | Malnutrition and Quality of Life in Chinese Cancer Patients: a Clinical Study of 23,994 Subjects显示文摘Background Malnutrition is common in patients with cancer,and can negatively impact their quality of life(QoL)and even survival.However,there is currently no large data available on the prevalence of malnutrition in Chinese cancer patients.This study evaluated the prevalence of malnutrition and the QoL of Chinese patients with locoregional,recurrent or metastatic cancer.Methods We conducted a nationwide observational,multi-center,hospital-based cross-sectional study within the Chinese Society of Nutritional Oncology(CSNO)Network.All of the patients were diagnosed with one of the following 18 different types of malignant tumors:lung cancer,gastric cancer,liver cancer,colorectal cancer,breast cancer,esophageal cancer,cervical cancer,endometrial cancer,nasopharyngeal carcinoma,malignant lymphoma,leukemia,pancreatic cancer,ovarian cancer,prostate cancer,bladder cancer,brain cancer,biliary tract malignant tumors or gastrointestinal stromal tumors.These patients were enrolled from 72 hospitals located in different regions of China.The patients’nutritional status was evaluated based on the body mass index(BMI),loss of bodyweight,laboratory measurements and patient generated-subjective global assessment(PG-SGA)scores.The cancer patients’physical status and QoL were assessed by the Karnofsky Performance Status(KPS)questionnaire and the European Organization for Research and Treatment of Cancer(EORTC)QLQ-C30 questionnaire,respectively.Results From December 2013 to April 2016,23,994 patients hospitalized for cancer treatment(such as surgery,chemotherapy or radiotherapy)were enrolled in the study.The patients included 12,494(52.9%)males and 11,124(47.1%)females.The mean age was 55.8±13.7 years.The proportions of patients in cancer stagesⅠ,Ⅱ,Ⅲ,Ⅳand uncertain were 11.5%,20.3%,27.5%,30.2%and 10.5%,respectively.Among the 23,994 inpatients,the proportions of patients who were underweight(BMI<18.5 kg/m2),normal(18.5 kg/m2 | Hong Xia Xu Chun Hua Song Zhen Ming Fu Chang Wang Zeng Qing Guo Yuan Lin Ying Ying Shi Wen Hu Yi Ba Su Yi Li Zeng Ning Li Kun Hua Wang Jing Wu Ying He Jia Jun Yang Cong Hua Xie Fu Xiang Zhou Xin Xia Song Gong Yan Chen Wen Jun Ma Su Xia Luo Zi Hua Chen Ming Hua Cong Hu Ma Chun Ling Zhou Wei Wang Qi Luo Yong Mei Shi Yu Mei Qi Hai Ping Jiang Wen Xian Guan Jun Qiang Chen Jia Xin Chen Yu Fang Lan Zhou Yong Dong Feng Rong Shao Tan Tao Li Jun Wen Ou Qing Chuan Zhao Jian Xiong Wu Min Weng Qing Hua Yao Jiu Wei Cui Wei Li Han Ping Shi the Investigation on Nutrition Status and Clinical Outcome of Common Cancers(INSCOC)Group | 2021 | Journal of Nutritional Oncology2021，6，1： | 8 |
| 6 | Mechanism of exogenous nucleic acids and their precursors improving the repair of intestinal epithelium after 7-irradiation in mice显示文摘AIM To clone expressed genes associated withrepair of irradiation-damaged mice intestinalgland cells treated by small intestinal RNA,andto explore the molecular mechanism ofexogenous nucleic acids improving repair ofintestinal crypt.METHODS The animal mode of test group andcontrol group was established,forty-five micebeing irradiated by γ ray were treated with smallintestinal RNA as test group,forty mice beingirradiated by γ ray were treated withphysiological saline as control group,five micewithout irradiation were used as normal control,their jejunal specimens were collectedrespectively at 6h,12h,24h,4d and 8d afterirradiation.Then by using LD-PCR based onsubtractive hybridization,these gene fragmentsdifferentially expressed between test group andcontrol group were obtained,and then werecloned into T vectors as well as beingsequenced.Obtained sequences were screenedagainst.GeneBank,if being new sequences,they were submitted to GeneBank.RESULTS Ninety clones were associated withrepair of irradiation-damaged intestinal glandcells treated by intestinal RNA.These clonesfrom test group of 6h,12h,24h,4d and 8dwere respectively 18,22,25,13,12.By screening against GeneBank,18 of which werenew sequences,the others were dramaticallysimilar to the known sequences,mainly similarto hsp,Nmi,Dutt1,alkaline phosphatase,homeobox,anti-CEA ScFv antibody,arginine/serine kinase and BMP-4,repA.Eighteen genefragments were new sequences,their acceptnumbers in GeneBank were respectivelyAF240164-AF240181.CONCLUSION Ninety clones were obtained tobe associated with repair of irradiation-damagedmice intestinal gland cells treated by smallintestinal RNA,which may be related toabnormal expression of genes and matchedproteins of hsp,Nmi,Duttl,Na,K-ATPase,alkalineph-osphatase,glkA,single strandedreplicative centromeric gene as well as 18 newsequences. | Da Xiang Cui~1 Guei Ying Zeng~2 Feng Wang~1 Jun Rong Xu~1 Dong Qing Ren~2 Yan Hai Guo~1 Fu Rong Tian~2 Xiao Jun Yan~1 Yu Hou~1 Cheng Zhi Su~1 1 Institute of Genetic Diagnosis of the Fourth Military Medical University,Xi’an 710032,China 2 Department of Irradiation Medicine of the Fourth Military Medical University,Xi’an 710032,China | 2000 | World Journal of Gastroenterology2000，6，5： | 8 |
| 7 | Increased Chondrocyte Apoptosis in Kashin-Beck Disease and Rats Induced by T-2 Toxin and Selenium Deficiency显示文摘Objective To investigate chondrocyte apoptosis and the expression of biochemical markers associated with apoptosis in Kashin-Beck disease(KBD) and in an established T-2 toxin-and selenium(Se) deficiency-induced rat model. Methods Cartilages were collected from the hand phalanges of five patients with KBD and five healthy children. Sprague-Dawley rats were administered a selenium-deficient diet for 4 weeks prior to T-2 toxin exposure. The apoptotic chondrocytes were observed by terminal deoxynucleotidyl transferase d UTP nick end labeling staining. Caspase-3, p53, Bcl-2, and Bax proteins in the cartilages were visualized by immunohistochemistry, their protein levels were determined by Western blotting, and m RNA levels were determined by real-time reverse transcription polymerase chain reaction. Results Increased chondrocyte apoptosis was observed in the cartilages of children with KBD. Increased apoptotic and caspase-3-stained cells were observed in the cartilages of rats fed with normal and Se-deficient diets plus T-2 toxin exposure compared to those in rats fed with normal and Se-deficient diets. Caspase-3, p53, and Bax proteins and m RNA levels were higher, whereas Bcl-2 levels were lower in rats fed with normal or Se-deficiency diets supplemented with T-2 toxin than the corresponding levels in rats fed with normal diet. Conclusion T-2 toxin under a selenium-deficient nutritional status induces chondrocyte death, which emphasizes the role of chondrocyte apoptosis in cartilage damage and progression of KBD. | YANG Hao Jie ZHANG Ying WANG Zhi Lun XUE Sen Hai LI Si Yuan ZHOU Xiao Rong ZHANG Meng FANG Qian WANG Wen Jun CHEN Chen DENG Xiang Hua CHEN Jing Hong | 2017 | Biomedical and Environmental Sciences2017，30，5： | 7 |
| 8 | The phylogenetic status of typical Chinese native pigs: analyzed by Asian and European pig mitochondrial genome sequences显示文摘China is one of the most diverse countries, which have developed 88 indigenous pig breeds. Several studies showed that pigs were independently domesticated in multiple regions of the world. The purpose of this study was to investigate the origin and evolution of Chinese pigs using complete mitochondrial genomic sequences (mtDNA) from Asian and European domestic pigs and wild boars. Thirty primer pairs were designed to determine the mtDNA sequences of Xiang pig, Large White, Lantang, Jinhua and Pietrain. The phylogenetic status of Chinese native pigs was investigated by comparing the mtDNA sequences of complete coding regions and D-loop regions respectively amongst Asian breeds, European breeds and wild boars. The analyzed results by two cluster methods contributed to the same conclusion that all pigs were classified into two major groups, European clade and Asian clade. It revealed that Chinese pigs were only recently diverged from each other and distinctly different from European pigs. Berkshire was clustered with Asian pigs and Chinese pigs were involved in the development of Berkshire breeding. The Malaysian wild boar had distant genetic relationship with European and Asian pigs. Jinhua and Lanyu pigs had more nucleotide diversity with Chinese pigs although they all belonged to the Asian major clade. Chinese domestic pigs were clustered with wild boars in Yangtze River region and South China. | Guanghui Yu Hai Xiang Jikun Wang Xingbo Zhao | 2013 | Journal of Animal Science and Biotechnology2013，4，2： | 7 |
| 9 | DNA cleavage mediated by water-soluble manganese corrole显示文摘水的 DNA 像核酸酶的活动可溶的锰 corrole 5, 10, 15-tris (N-methyl-4-pyridyl )- corrolatomanganese (III )(MnIIITMPyC ) 被调查了。MnIIITMPyC 可以经由由吸收系列,粘性大小和 CD 系列显示了的外面的沟绑定模式强烈绑在 DNA。MnIIITMPyC 面对 hydroperoxide 在 DNA 氧化劈开展出了优秀催化活动。 | Jun Lu Hai Yang Liu Lei Shi Xiang Li Wang Xiao Ying Lei Zhang Liang Nian Ji Lin Quan Zang Chi K. Chang | 2011 | Chinese Chemical Letters2011，22，1： | 6 |
| 10 | Preparation and characterization of porous TiO_2/ZnO composite nanofibers via electrospinning显示文摘多孔的 TiO2/ZnO 合成 nanofibers 被 electrospinning 技术成功地第一次准备了。它被锻烧 TiO2/ZnCl2/PVP 产生[PVP:poly (乙烯基 pyrrolidone )] nanofibers,是从 TiO2 的一个混合答案的 electrospun, ZnCl2 和 PVP。传播电子显微镜学(TEM ) 和衍射(XRD ) 分析的 X 光检查被用来识别 TiO2/ZnO nanofibers 和无机的 TiO2/ZnO 纤维的形成的形态学。TiO2/ZnO 纤维的多孔的结构被 N2 adsoption/desorption 等温线描绘。表面 photovoltage 光谱学(SPS ) 和揭示的大小预付多孔的 TiO2/ZnO 合成 nanofibers 和结果的性质的 photocatalytic 活动与纯 TiO2 nanofibers,纯 ZnO nanofibers 和 TiO2/ZnO nanoparticles 相比。 | Hai Ying Wang Yang Yang Xiang Li Li Juan Li Ce Wang | 2010 | Chinese Chemical Letters2010，21，9： | 5 |
| 11 | Wrinkled petals and stamens 1,is required for the morphogenesis of petals and stamens in Lotus japonicus显示文摘尽管许多进步在理解花的机关身份怎么在花的发展期间是坚定的被取得了,更少花的机关怎么在晚花的发展阶段被详细描述被知道。这里,我们描述新奇花的异种,起皱的花瓣和 stamens1 (wps1 ) ,它在花瓣和雄蕊的发展显示出缺点。基因分析显示那 wps1 异种相应于在染色体 3 的长手臂的一个单个后退的地点。在 wps1 异种的花的机关的早发展在野类型类似于那,并且异种的 malfunction 在晚发展的阶段开始,显示花瓣和雄蕊的外观上的一个缺点。在成熟的花,在异种的花瓣和雄蕊细丝被起皱或合拢,并且在花瓣和雄蕊细丝的 L1 层下面的细胞的形态学是反常的。花的机关身份基因的表达式模式没与野类型的相比在 wps1 异种被影响,这被发现,与所有花的机关的未改变的开发一致。而且,在花瓣的不同类型的表皮的房间的身份被维持。组织学的分析证明在 wps1 花,所有花瓣不规则地被合拢,并且在花瓣有系的结构,当内部房间的形状和安排畸形、未组织起来时。把结果基于这些,我们建议 Wps1 下游地行动到班 B 花的机关身份基因,并且工作在迟了的花期间调制细胞的区别发展阶段。 | Jiang Hua Chen Ji Liang Pang Li Lin Wang Yong Hai Luo Xin Li Xiang Ling Cao Kui Lin Wei Ma Xiao He Hu Da Luo | 2006 | Cell Research2006，16，5： | 4 |
| 12 | Translational Regulation of Plant Response to High Temperature by a Dual-Function tRNAHls Guanylyltransferase in Rice显示文摘As sessile organisms,plants have evolved numerous strategies to acclimate to changes in environmental temperature.However,the molecular basis of this acclimation remains largely unclear.In this study we identified a tRNAHis guanylyltransferase,AET1,which contributes to the modification of pre-tRNAH,s and is required for normal growth under high-temperature conditions in rice.Interestingly,AET1 possibly interacts with both RACK1A and elF3h in the endoplasmic reticulum.Notably,AET1 can directly bind to OsARF mRNAs including the uORFs of OsARF19 and OsARF23,indicating that AET1 is associated with translation regulation.Furthermore,polysome profiling assays suggest that the translational status remains unaffected in the aet1 mutant,but that the translational efficiency of OsARF19 and OsARF23 is reduced;moreover,OsARF23 protein levels are obviously decreased in the aet1 mutant under high temperature,implying that AET1 regulates auxin signaling in response to high temperature.Ourfindings provide new insights into the molecular mechanisms whereby AET1 regulates the environmental temperature response in rice by playing a dual role in tRNA modification and translational control. | Ke Chen Tao Guo Xin-Min Li Yi-Min Zhang Yi-Bing Yang Wang-Wei Ye Nai-Qian Dong Chuan-Lin Shi Yi Kan You-Huang Xiang Hai Zhang Ya-Chao Li Ji-Ping Gao Xuehui Huang Qiang Zhao Bin Han Jun-Xiang Shan Hong-Xuan Lin | 2019 | Molecular Plant2019，12，8： | 4 |
| 13 | Asymmetric reduction of α-hydroxy aromatic ketones to chiral aryl vicinal diols using carrot enzymes system显示文摘-hydroxy 的不对称的减小芳香的酉同类被使用胡萝卜酶系统执行,让步的相应 chiral 与特殊的功能的组一起的附近的 diols。最佳反应条件在各种各样的影响因素的调查以后被获得。Chiral 芳基附近的 diols 在适当条件下面与好收益和优秀 enantiomeric 过量被生产。同时,位的因素和芳香的戒指上的取代者的电子效果被显示在产量和 enantioselectivity 上有有趣的影响。 | Xiang Liu Yi Wang Hai Yan Gao Jian He Xu | 2012 | Chinese Chemical Letters2012，23，6： | 4 |
| 14 | Mitochondrial DNA T7719G in tRNA-Lys gene affects litter size in Small-tailed Han sheep显示文摘Background: In farm animals, mitochondrial DNA(mtDNA) effect on economic performance remains hot-topic for breeding and genetic selection. Here, 53 maternal lineages of Small-tailed Han sheep were used to investigate the association of mitochondrial DNA variations and the lambing litter size.Results: Sequence sweeping of the mitochondrial coding regions discovered 31 non-synonymous mutations, and the association study revealed that T7719G in mt DNA t RNA-Lys gene was associated with litter size(P < 0.05),manifesting 0.29 lambs per litter between the G and T carriers. Furthermore, using the mixed linear model, we assayed the potential association of the ovine litter size and haplogroups and multiple-level mtDNA haplotypes,including general haplotypes, assembled haplotypes of electron transport chain contained sequences(H-ETC),mitochondrial respiratory complex contained sequences(H-MRC) and mitochondrial genes(H-gene, including polypeptide-coding genes, rRNA genes and tRNA genes). The strategy for assembled mitochondrial haplotypes was proposed for the first time in mtDNA association analyses on economic traits, although none of the significant relations could be concluded(P > 0.05). In addition, the nuclear major gene BMPR1B was significantly correlated with litter size in the flock(P < 0.05), however, did not interact with mtDNA T7719G mutation(P > 0.05).Conclusions: Our results highlight mutations of ovine mitochondrial coding genes, suggesting T7719G in tRNA-Lys gene be a potentially useful marker for selection of sheep litter size. | Xiaoyong Chen Dan Wang Hai Xiang Weitao Dun Dave O.H.Brahi Tao Yin Xingbo Zhao | 2017 | Journal of Animal Science and Biotechnology2017，8，3： | 4 |
| 15 | Loading necrostatin-1 composite bone cement inhibits necroptosis of bone tissue in rabbit显示文摘Bone necrosis after injecting of polymethylmethacrylate(PMMA)bone cement will lead to re-fracture of bone tissue.As a new type of necrosis,there is little research related to the necroptosis of surrounding bone tissue near the bone cement.The purpose of our study was to(i)investigate the presence of necroptosis in vivo and,(ii)established as a new type of bone cement containing PMMA,calcium phosphate cement(CPC)and Necrostatin-1(Nec-1)to inhibit necroptosis of bone tissue.A total of 12 Japanese rabbits were used to establish the animal model and randomly divided into 4 groups signed as a control group,PMMA group,PMMA–CPC group and PMMA–CPC–Nec-1 group,respectively.We used scanning electron microscope to observe the structure of the samples,used HE staining to detect the necrosis,and used western blotting as well as ELISA test to examine the iconic molecule receptor interacting protein kinase-3(RIP 3)protein and tumor necrosis factor a(TNF-a).After analyzing the results of our study,we found that the structure in both PMMA bone cement group and composite bone cement group was damaged and there was an evidence of necrosis,but it was absent in control group.Through molecule detection,the RIP 3 protein expression was decreased in PMMA–CPC–Nec-1(P<0.05).TNF-a expression was increased in bone cement groups with and without CPC(P<0.05),but was inhibited in PMMA–CPC–Nec-1 group.We have concluded that the necroptosis could be confirmed in bone tissue necrosis induced by TNF-a after bone cement injection and also could be inhibited by composite bone cement with Nec-1. | Xiang Ji Feng Xu Guoling Dong Chongzhe Jia Pu Jia Hao Chen Hai Tang | 2019 | Regenerative Biomaterials2019，6，2： | 4 |
| 16 | Solidified structure of thin-walled titanium parts by vertical centrifugal casting显示文摘The solidified structure of the thin-walled and complicated Ti-6Al-4V castings produced by the vertical centrifugal casting process was studied in the present work.The results show that the wall thickness of the section is featured with homogeneously distributed fine equiaxial grains,compared with the microstructure of the thick-walled section.The grain size of the castings has a tendency to decrease gradually with the increasing of the centrifugal radius.The inter-lamellar space in thick-walled casting parts is bigger than that of the thin-walled parts,and the profile of inter-lamellar space is not susceptible to the centrifugal radius. | Wu Shiping Xu Qin Zhang Jun Nan Hai Xue Xiang Guo Jingjie | 2011 | China Foundry2011，8，2： | 4 |
| 17 | Purification function of the natural wetland in the Liaohe Delta显示文摘1IntroductionStructure,functionandchangearethreeimportantaspectsoflandscapeecology(Forman,1986).Landscapefunctionmeanstheinte... | Li Xiu Zhen, Qu Xiang rong, Wang Lian ping, Zhang Hai rong, Xiao Du ning Institute of Applied Ecology, Chinese Academy of Sciences, B.O. Box 417, Shengyang 110015, China | 1999 | Journal of Environmental Sciences1999，11，2： | 4 |
| 18 | Crystal structure and DNA binding studies of a cobalt(Ⅱ) complex containing mixed-ligands of 1,10-phenathroline and glycollic acid显示文摘<正>A cobalt(11) complex containing mixed-ligands of 1,10-phenathroline(phen) and glycollic acid(GA).|Co(GA)_2(phen)| was synthesized and its structure was characterized by X-ray diffraction.The interaction of the complex with DNA was investigated by electronic absorption spectra and electrochemical methods.Electronic absorption spectrum experiments showed that after interaction with DNA.the characteristic absorption peaks of |Co(GA)_2(phen)| underwent hypochromic effect as well as redshift. Also,the binding strength of 3.8×10~4L/mol was estimated by titration method.Electrochemical assays revealed that the redox peak currents of the complex decreased obviously accompanied by a positive shift of the formal potential after association with DNA.All these results revealed that the synthesized cobalt complex bound with DNA via an intercalation mode. | Hai Bin Lin Qing Xiang Wang Cong Min Zhang Wen Qi Li | 2011 | Chinese Chemical Letters2011，22，8： | 3 |
| 19 | Immunological Evaluation of a Novel Mycobacterium tuberculosis Antigen Rv0674显示文摘Objective This study aimed to characterize the diagnostic and vaccine potential of a novel Mycobacterium tuberculosisantigen Rv0674. Methods To evaluate thediagnostic potential and antigenicity of Rv0674, IgG was evaluated using ELISA and interferon (IFN)-γ was done by using ELISpot assay among TB patients and healthy donors. For immunogenicity evaluation, BALB/c mice were immunized with Rv0674. Cytokine production was determined by cytokine release assay using an ELISA kit, and the antibodies were tested using ELISA. Results The results of serum Elisa tests showed that Rv0674 specific immunoglobulin G (IgG) response was higher in TB patients than negative controls. And Rv0674 had good performance in serological test with sensitivity and specificity of 77.1% and 81.1%, respectively. While it shows poor sensitivity and specificity of 26.23% and 79.69% for IFN-γ tests. In BALB/c mice, Rv0674 adjuvant by DDA/PolyI:C could also induce a high level of IFN-γ, interleukin-2 and interleukin-6 as well as a high IgG titer in both high-and low-dose groups indicating that Rv0674 is essential in humoral and cellular immunity. Moreover, the cytokine profile and IgG isotypecharacterized Rv0674 as a Th1/Th2-mixed-type protective immunity with the predominance of Th1 cytokines. Conclusion Rv0674 may be a good potential candidate for the development of TB serological diagnosis and a new TB vaccine. | XIAO Tong Yang LIU Hai Can LI Xiao Qin HUANG Ming Xiang LI Gui Lian LI Na YAN Yu Han LUO Qiao WANG Xue Zhi LI Ma Chao WAN Kang Lin | 2019 | Biomedical and Environmental Sciences2019，32，6： | 3 |
| 20 | Reductive demetalation of manganese corroles:The substituent effect显示文摘锰 corroles 的减少的 demetalation 在 CH2Cl2/HCl 被调查(水) 由把 SnCl2 用作减少代理人的溶剂。demetalation 收益显著地取决于 corrole macrocycle 的取代者,这被发现。充满电子的锰 corrole 比电子缺乏的更容易经历减少的 demetalation。锰 5,10,15-tris (苯基) 的孤立的减少的 demetalation 收益在现在的系统的 corrole 是中等的(46%) 。至于电子缺乏的 Mn (III ) 5,10,15-tris (pentafluorophenyl ) corrole,在 HOAc 鈥揌2 SO4 (V/V = 3:1 ) 的导致酸的 demetalation 与 67% 的孤立的收益更好。 | Hai Yang Liu Ling Chen Fei Yam Hai Ying Zhan Xiao Ying Xiang Li Wang Huan Feng Jiang Chi Kwong Chang | 2008 | Chinese Chemical Letters2008，19，8： | 3 |
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