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| 1 | Lineage tracing of direct astrocyte-to-neuron conversion in the mouse cortex显示文摘Regenerating functional new neurons in the adult mammalian central nervous system has been proven to be very challenging due to the inability of neurons to divide and repopulate themselves after neuronal loss.Glial cells,on the other hand,can divide and repopulate themselves under injury or diseased conditions.We have previously reported that ectopic expression of NeuroD1 in dividing glial cells can directly convert them into neurons.Here,using astrocytic lineage-tracing reporter mice(Aldh1l1-CreERT2 mice crossing with Ai14 mice),we demonstrate that lineage-traced astrocytes can be successfully converted into NeuNpositive neurons after expressing NeuroD1 through adeno-associated viruses.Retroviral expression of NeuroD1 further confirms that dividing glial cells can be converted into neurons.Importantly,we demonstrate that for in vivo cell conversion study,using a safe level of adeno-associated virus dosage(10^10–10^12 gc/mL,1μL)in the rodent brain is critical to avoid artifacts caused by toxic dosage,such as that used in a recent bioRxiv study(2×10^13 gc/mL,1μL,mouse cortex).For therapeutic purpose under injury or diseased conditions,or for non-human primate studies,adeno-associated virus dosage needs to be optimized through a series of dose-finding experiments.Moreover,for future in vivo gliato-neuron conversion studies,we recommend that the adeno-associated virus results are further verified with retroviruses that mainly express transgenes in dividing glial cells in order to draw solid conclusions.The study was approved by the Laboratory Animal Ethics Committee of Jinan University,China(approval No.IACUC-20180330-06)on March 30,2018. | Zongqin Xiang Liang Xu Minhui Liu Qingsong Wang Wen Li Wenliang Lei Gong Chen | 2021 | Neural Regeneration Research2021，16，4： | 9 |
| 2 | Decreased expression of protease-activated receptor 4 in human gastric cancer显示文摘 | Yong Zhang Guoyu Yu Ping Jiang Yang Xiang Wenliang Li Wenhui Lee Yun Zhang | 2011 | International Journal of Biochemistry and Cell Biology2011，，9： | 2 |
| 3 | Community of extremely halophilic bacteria in historic Dagong Brine Well in southwestern China显示文摘 | Wenliang Xiang Jianhua Guo Wei Feng Min Huang Hao Chen Jian Zhao Jie Zhang Zhirong Yang Qun Sun | 2008 | World Journal of Microbiology and Biotechnology2008，，10： | 1 |
| 4 | Molecular Characterization of Drug-Resistant Mycobacterium tuberculosis Isolates from Sichuan Province in China 显示文摘 | Guo Jianhua Xiang Wenliang Zhao Qingrong | 2008 | Jpn J Infect Dis2008，6，： | 1 |
| 5 | Isolation andperformance evaluation of halotolerant phosphate solubilizing bacteria fromthe rhizospheric soils of historic Dagong Brine Well in China显示文摘 | XIANG Wenliang LIANG Huazhong LIU Sen | 2011 | WorldJournal of Microbiology and Biotechnology2011，27，11： | 1 |
| 6 | Community of extremely halophilic bacteria in historic Dagong Brine Well in southwestern China显示文摘 | XIANG Wenliang GUO Jianhua FENG Wei | 2008 | World Journal of Microbiology and Biotechnology2008，24，： | 1 |
| 7 | Isolation and performance evaluation of halotolerant phosphate solubilizing bacteria from the rhizospheric soils of historic Dagong Brine Well in China显示文摘 | XIANG Wenliang LIANG Huazhong LIU Sen | | 0，，11： | 1 |
| 8 | Large transverse thermoelectric figure of merit in a topological Dirac semimetal显示文摘The Seebeck effect encounters a few fundamental constraints hindering its thermoelectric(TE)conversion efficiency.Most notably,there are the charge compensation of electrons and holes that diminishes this effect,and the Wiedemann-Franz(WF)law that makes independent optimization of the corresponding electrical and thermal conductivities impossible.Here,we demonstrate that in the topological Dirac semimetal Cd3As2 the Nernst effect,i.e.,the transverse counterpart of the Seebeck effect,can generate a large TE figure of merit zNT.At room temperature,zNT≈0.5 in a small field of 2 T and it significantly surmounts its longitudinal counterpart for any field.A large Nernst effect is generically expected in topological semimetals,benefiting from both the bipolar transport of compensated electrons and holes and their high mobilities.In this case,heat and charge transport are orthogonal,i.e.,not intertwined by the WF law anymore.More importantly,further optimization of zNT by tuning the Fermi level to the Dirac node can be anticipated due to not only the enhanced bipolar transport,but also the anomalous Nernst effect arising from a pronounced Berry curvature.A combination of the topologically trivial and nontrivial advantages promises to open a new avenue towards high-efficient transverse thermoelectricity. | JunSen Xiang SiLe Hu Meng Lyu WenLiang Zhu ChaoYang Ma ZiYu Chen Frank Steglich GenFu Chen PeiJie Sun | 2020 | Science China(Physics,Mechanics & Astronomy)2020，63，3： | 0 |
| 9 | Differentiate Xp11.2 Translocation Renal Cell Carcinoma from Computed Tomography Images and Clinical Data with ResNet-18 CNN and XGBoost显示文摘This study aims to apply ResNet-18 convolutional neural network(CNN)and XGBoost to preoperative computed tomography(CT)images and clinical data for distinguishing Xp11.2 translocation renal cell carcinoma(Xp11.2 tRCC)from common subtypes of renal cell carcinoma(RCC)in order to provide patients with individualized treatment plans.Data from45 patients with Xp11.2 tRCC fromJanuary 2007 to December 2021 are collected.Clear cell RCC(ccRCC),papillary RCC(pRCC),or chromophobe RCC(chRCC)can be detected from each patient.CT images are acquired in the following three phases:unenhanced,corticomedullary,and nephrographic.A unified framework is proposed for the classification of renal masses.In this framework,ResNet-18 CNN is employed to classify renal cancers with CT images,while XGBoost is adopted with clinical data.Experiments demonstrate that,if applying ResNet-18 CNN or XGBoost singly,the latter outperforms the former,while the framework integrating both technologies performs similarly or better than urologists.Especially,the possibility of misclassifying Xp11.2 tRCC,pRCC,and chRCC as ccRCC by the proposed framework is much lower than urologists. | Yanwen Lu Wenliang Ma Xiang Dong Mackenzie Brown Tong Lu Weidong Gan | 2023 | Computer Modeling in Engineering & Sciences2023，，7： | 0 |
| 10 | Two-photon live imaging of direct glia-to-neuron conversion in the mouse cortex显示文摘Over the past decade,a growing number of studies have reported transcription factor-based in situ reprogramming that can directly conve rt endogenous glial cells into functional neurons as an alternative approach for n euro regeneration in the adult mammalian central ne rvous system.Howeve r,many questions remain regarding how a terminally differentiated glial cell can transform into a delicate neuron that forms part of the intricate brain circuitry.In addition,concerns have recently been raised around the absence of astrocyte-to-neuron conversion in astrocytic lineage-tra cing mice.In this study,we employed repetitive two-photon imaging to continuously capture the in situ astrocyte-to-neuron conversion process following ecto pic expression of the neural transcription factor NeuroD1 in both prolife rating reactive astrocytes and lineage-tra ced astrocytes in the mouse cortex.Time-lapse imaging over several wee ks revealed the ste p-by-step transition from a typical astrocyte with numero us short,tapered branches to a typical neuro n with a few long neurites and dynamic growth cones that actively explored the local environment.In addition,these lineage-converting cells were able to migrate ra dially or to ngentially to relocate to suitable positions.Furthermore,two-photon Ca2+imaging and patch-clamp recordings confirmed that the newly generated neuro ns exhibited synchronous calcium signals,repetitive action potentials,and spontaneous synaptic responses,suggesting that they had made functional synaptic connections within local neural circuits.In conclusion,we directly visualized the step-by-step lineage conversion process from astrocytes to functional neurons in vivo and unambiguously demonstrated that adult mammalian brains are highly plastic with respect to their potential for neuro regeneration and neural circuit reconstruction. | Zongqin Xiang Shu He Rongjie Chen Shanggong Liu Minhui Liu Liang Xu Jiajun Zheng Zhouquan Jiang Long Ma Ying Sun Yongpeng Qin Yi Chen Wen Li Xiangyu Wang Gong Chen Wenliang Lei | 2024 | Neural Regeneration Research2024，19，8： | 0 |
| 11 | Determination of Double Beta Decay Half-Life of 136Xe with the PandaX-4T Natural Xenon Detector显示文摘Precise measurement of two-neutrino double beta decay(DBD)half-life is an important step for the searches of Majorana neutrinos with neutrinoless double beta decay.We report the measurement of DBD half-life of 136xe using the Pandax-4T dual-phase Time Projection Chamber(TPC)with 3.7-tonne natural xenon and the first 94.9-day physics data release. | Lin Si Zhaokan Cheng Abdusalam Abdukerim Zihao Bo Wei Chen Xun Chen Yunhua Chen Chen Cheng Yunshan Cheng Xiangyi Cui Yingjie Fan Deqing Fang Changbo Fu Mengting Fu Lisheng Geng Karl Giboni Linhui Gu Xuyuan Guo Ke Han Changda He Jinrong He Di Huang Yanlin Huang Zhou Huang Ruquan Hou Xiangdong Ji Yonglin Ju Chenxiang Li Jiafu Li Mingchuan Li Shu Li Shuaijie Li Qing Lin Jianglai Liu Xiaoying Lu Lingyin Luo Yunyang Luo Wenbo Ma Yugang Ma Yujun Mao Yue Meng Nasir Shaheed Xiaofeng Shang Xuyang Ning Ningchun Qi Zhicheng Qian Xiangxiang Ren Changsong Shang Guofang Shen Wenliang Sun Andi Tan Yi Tao Anqing Wang Meng Wang Qiuhong Wang Shaobo Wang Siguang Wang Wei Wang Xiuli Wang Zhou Wang Yuehuan Wei Mengmeng Wu Weihao Wu Jingkai Xia Mengjiao Xiao Xiang Xiao Pengwei Xie Binbin Yan Xiyu Yan Yong Yang Chunxu Yu Jumin Yuan Ying Yuan Zhe Yuan Dan Zhang Minzhen Zhang Peng Zhang Shibo Zhang Shu Zhang Tao Zhang Li Zhao Qibin Zheng Jifang Zhou Ning Zhou Xiaopeng Zhou Yong Zhou | 2023 | Research2023，，2： | 0 |
| 12 | OPTIMUMDESIGNOFSTREAMLINEDEXTRUSIONDIESBASEDONUPPERBOUNDMETHODANALYSIS显示文摘提出了一种通用且形状变化丰富的流线型挤压模曲面的表示方法,流线型挤压模曲面用毛坯和型材截面形状的插值来表示。毛坯和型材截面形状由带张力的曲线拟合,通过调整张力参数以拟合带有直线段的型材截面。材料在模具内的流动由上限法模拟,通过极小化挤压力优化模具曲面和动可容速度中的伪独立参数,以获得最佳模具曲面。最后讨论了摩擦条件、模具长度、面积缩减率。 | Huang Xiang Chen Wenliang Zhou Rurong Department of Mechanical Engineering,NUAA29 Yudao Street, Nanjing 210016, P. R. China | 1996 | Transactions of Nanjing University of Aeronautics and Astronautics1996，13，1： | 0 |
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