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| 1 | Ultrafast epitaxial growth of metre-sized single-crystal graphene on industrial Cu foil显示文摘A foundation of the modern technology that uses single-crystal silicon has been the growth of highquality single-crystal Si ingots with diameters up to 12 inches or larger. For many applications of graphene, large-area high-quality(ideally of single-crystal) material will be enabling. Since the first growth on copper foil a decade ago, inch-sized single-crystal graphene has been achieved. We present here the growth, in 20 min, of a graphene film of(5 ×50) cm^2 dimension with >99% ultra-highly oriented grains.This growth was achieved by:(1) synthesis of metre-sized single-crystal Cu(1 1 1) foil as substrate;(2)epitaxial growth of graphene islands on the Cu(1 1 1) surface;(3) seamless merging of such graphene islands into a graphene film with high single crystallinity and(4) the ultrafast growth of graphene film.These achievements were realized by a temperature-gradient-driven annealing technique to produce single-crystal Cu(1 1 1) from industrial polycrystalline Cu foil and the marvellous effects of a continuous oxygen supply from an adjacent oxide. The as-synthesized graphene film, with very few misoriented grains(if any), has a mobility up to ~23,000 cm^2 V^(-1)s^(-1)at 4 K and room temperature sheet resistance of ~230 Ω/□. It is very likely that this approach can be scaled up to achieve exceptionally large and high-quality graphene films with single crystallinity, and thus realize various industrial-level applications at a low cost. | Xiaozhi Xu Zhihong Zhang Jichen Dong Ding Yi Jingjing Niu Muhong Wu Li Lin Rongkang Yin Mingqiang Li Jingyuan Zhou Shaoxin Wang Junliang Sun Xiaojie Duan Peng Gao Ying Jiang Xiaosong Wu Hailin Peng Rodney S. Ruoff Zhongfan Liu Dapeng Yu Enge Wang Feng Ding Kaihui Liu | 2017 | Science Bulletin2017，62，15： | 40 |
| 2 | Expression changes and bioinformatic analysis of Wallerian degeneration after sciatic nerve injury in rat显示文摘Wallerian degeneration (WD) remains an important research topic. Many genes are differentially expressed during the process of WD, but the precise mechanisms responsible for these differentiations are not completely understood. In this study, we used microarrays to analyze the expression changes of the distal nerve stump at 0, 1, 4, 7, 14, 21 and 28 days after sciatic nerve injury in rats. The data revealed 6 076 differentially-expressed genes, with 23 types of expression, specifically enriched in genes associated with nerve development and axonogenesis, cytokine biosynthesis, cell differentiation, cytokine/chemokine production, neuron differentiation, cytokinesis, phosphorylation and axon regeneration. Kyoto Encyclopedia of Genes and Genomes pathway analysis gave findings related mainly to the MAPK signaling pathway, the Jak-STAT signaling pathway, the cell cycle, cytokine-cytokine receptor interaction, the p53 signaling pathway and the Wnt signaling pathway. Some key factors were NGF, MAG, CNTF, CTNNA2, p53, JAK2, PLCB1, STAT3, BDNF, PRKC, collagen II, FGF, THBS4, TNC and c-Src, which were further validated by real-time quantitative PCR, Western blot, and immunohistochemistry. Our findings contribute to a better understanding of the functional analysis of differentially-expressed genes in WD and may shed light on the molecular mechanisms of nerve degeneration and regeneration. | Dengbing Yao Meiyuan Li Dingding Shen Fei Ding Shibi Lu Qing Zhao Xiaosong Gu | 2013 | Neuroscience Bulletin2013，29，3： | 18 |
| 3 | Single-cell RNA sequencing in breast cancer: Understanding tumor heterogeneity and paving roads to individualized therapy显示文摘Single-cell RNA sequencing(scRNA-seq)is a novel technology that allows transcriptomic analyses of individual cells.During the past decade,scRNA-seq sensitivity,accuracy,and efficiency have improved due to innovations including more sensitive,automated,and cost-effective single-cell isolation methods with higher throughput as well as ongoing technological development of scRNA-seq protocols.Among the variety of current approaches with distinct features,researchers can choose the most suitable method to carry out their research.By profiling single cells in a complex population mix,scRNA-seq presents great advantages over traditional sequencing methods in dissecting heterogeneity in cell populations hidden in bulk analysis and exploring rare cell types associated with tumorigenesis and metastasis.scRNA-seq studies in recent years in the field of breast cancer research have clustered breast cancer cell populations with different molecular subtypes to identify distinct populations that may correlate with poor prognosis and drug resistance.The technology has also been used to explain tumor microenvironment heterogeneity by identifying distinct immune cell subsets that may be associated with immunosurveillance and are potential immunotherapy targets.Moreover,scRNA-seq has diverse applications in breast cancer research besides exploring heterogeneity,including the analysis of cell-cell communications,regulatory single-cell states,immune cell distributions,and more.scRNA-seq is also a promising tool that can facilitate individualized therapy due to its ability to define cell subsets with potential treatment targets.Although scRNA-seq studies of therapeutic selection in breast cancer are currently limited,the application of this technology in this field is prospective.Joint efforts and original ideas are needed to better implement scRNA-seq technologies in breast cancer research to pave the way for individualized treatment management.This review provides a brief introduction on the currently available scRNA-seq approaches along with their corresponding strengths and weaknesses and may act as a reference for the selection of suitable methods for research.We also discuss the current applications of scRNA-seq in breast cancer research for tumor heterogeneity analysis,individualized therapy,and the other research directions mentioned above by reviewing corresponding published studies.Finally,we discuss the limitations of current scRNA-seq technologies and technical problems that remain to be overcome. | Shuning Ding Xiaosong Chen Kunwei Shen | 2020 | Cancer Communications2020，40，8： | 12 |
| 4 | Non-coding RNAs as Emerging Regulators of Neural Injury Responses and Regeneration显示文摘Non-coding RNAs(ncRNAs) are a large cluster of RNAs that do not encode proteins,but have multiple functions in diverse cellular processes.Mounting evidence indicates the involvement of nc RNAs in the physiology and pathophysiology of the central and peripheral nervous systems.It has been shown that numerous ncRNAs,especially micro RNAs and long non-coding RNAs,are differentially expressed after insults such as acquired brain injury,spinal cord injury,and peripheral nerve injury.These ncRNAs affect neuronal survival,neurite regrowth,and glial phenotype primarily by targeting specific mRNAs,resulting in translation repression or degradation of the mRNAs.An increasing number of studies have investigated the regulatory roles of micro RNAs and long non-coding RNAs in neural injury and regeneration,and thus a new research field is emerging.In this review,we highlight current progress in the field in an attempt to provide further insight into post-transcriptional changes occurring after neural injury,and to facilitate the potential use of ncRNAs for improving neural regeneration.We also suggest potential directions for future studies. | Songlin Zhou Fei Ding Xiaosong Gu | 2016 | Neuroscience Bulletin2016，32，3： | 12 |
| 5 | Altered microRNA expression following sciatic nerve resection in dorsal root ganglia of rats显示文摘MicroRNAs (miRNAs ) 是否定地 post-transcriptionally 调整基因表示的小、非编码的 RNA (22 核苷酸) 的一个类,通过目标 mRNAs 的翻译抑制或降级的任何一个。我们揭开了在在在在在用 microarray 分析的老鼠的臀部的神经的切除术以后的 1, 4, 7,和 14 天的背面的根 ganglia (DRG ) 的 miRNAs 的表示的以前未知的改变。32 显著地, upregulated 和 18 downregulated miRNAs 在跟随臀部的神经损害的四个次点在 DRG 被识别。四的表示连续地 deregulated miRNAs,由即时 Taqman 聚合酶链反应分析了,与 microarray 数据一致(upregulated:miR-21, miR-221;downregulated:miR-500, miR-551b ) 。为这些 miRNAs 的潜在的目标,在臀部的神经切除术以后改变了,主要涉及细胞的进程的神经系统开发,多细胞的 organismal 开发,和规定。这研究在一只老鼠在臀部的神经的切除术以后在 DRG 表明了 miRNAs 的不同参与模型,和它可以也在说明为神经新生负责的分子的机制作出贡献。 | Bin Yu Songlin Zhou Tianmei Qian Yongjun Wang Fei Ding Xiaosong Gu | 2011 | Acta Biochimica et Biophysica Sinica2011，43，11： | 9 |
| 6 | Dog sciatic nerve gap repaired by artificial tissue nerve graft显示文摘The feasibility of repairing dog sciatic nerve damage by using a biodegradable artificial tissue nerve graft enriched with neuroregenerating factors is investigated. The artificial nerve graft was implanted to a 30 mm gap of the sciatic nerve damage in 7 dogs. The dogs with the same nerve damage that were repaired by interposition of the autologous nerve or were given no treatment served as control group 1 or 2, respectively. The observations include gross and morphological observations, immune reaction, electrophysiological examination, fluorescence tracing of the neuron formation and the number of the neurons at the experimental sites, etc. Results showed that 6 months after the implantation of the graft, the regenerated nerve repaired the damage of the sciatic nerve without occurrence of rejection and obvious inflammatory reaction in all 7 dogs, and the function of the sciatic nerve recovered with the nerve conduction velocity of (23.91±11.35)m/s. The regenerated neurons and the forming of axon could be observed under an electron microscope. This proves that artificial tissue nerve graft transplantation can bridge the damaged nerve ends and promote the nerve regeneration. | GU Xiaosong, ZHANG Peiyun, WANG Xiaodong, DING Fei, PENG Luping and CHENG Hongbing(Nantong Medical College, The Key Laboratory of Neuroregeneration of Jiangsu Province, Nantong 226001, China) | 2003 | Progress in Natural Science:Materials International2003，13，8： | 6 |
| 7 | Biocompatibility studies of silk fibroin-based artificial nerve grafts in vitro and in vivo显示文摘Silk fibroin(SF)has been used extensively in the biomedical field including tissue engineering for the generation of artificial bones,skins or ligaments.We have previously reported on good in vitro biocompatibility of SF fibers with peripheral nerve tissues and cells.In the present study,we developed a novel design of the SF-based artificial nerve graft(SF graft)which was composed of a SF-nerve guidance conduit(NGC)inserted with SF fibers.MTT assay was performed to determine the cytotoxicity of the SF-NGC extract fluid on the cultured L929 cells derived from an immortalized mouse fibroblast cell line.In addition,this SF graft was implanted into adult rats for bridging a 10-mm long sciatic nerve defect.The following-up experiments at initial stage(1-4 week)of nerve regeneration including routine blood tests and histochemical investigation were conducted to evaluate the in vivo biocompatibility of the SF graft with peripheral nerves.The results demonstrated that the SF-NGC graft was biocompatible with the surrounding tissues and cells due to its low inflammatory potential with a grade 0 under the U.S.Pharmacopeia guidelines and it was generally suitable to a certain degree for bridging peripheral nerve defects in virtue of supporting Schwann cell adherence,expansion and migration.Therefore the SF graft is a promising alternative to classical autografts for peripheral nerve repair. | Chen Xuemei Yang Yumin Wu Jian Zhao Yahong Ding Fei Gu Xiaosong | 2007 | Progress in Natural Science:Materials International2007，17，9： | 5 |
| 8 | The Achyranthes bidentata polypeptide k fraction enhances neuronal growth in vitro and promotes peripheral nerve regeneration after crush injury in vivo显示文摘We have previously shown that Achyranthes bidentata polypeptides(ABPP), isolated from Achyranthes bidentata Blume(a medicinal herb), exhibit neurotrophic and neuroprotective effects on the nervous system. To identify the major active component of ABPP, and thus optimize the use of ABPP, we used reverse-phase high performance liquid chromatography to separate ABPP. We obtained 12 fractions, among which the fraction of ABPPk demonstrated the strongest neuroactivity. Immunocytochemistry and western blot analysis showed that ABPPk promoted neurite growth in cultured dorsal root ganglion explant and dorsal root ganglion neurons, which might be associated with activation of Erk1/2. A combination of behavioral tests, electrophysiological assessment, and histomorphometric analysis indicated that ABPPk enhanced nerve regeneration and function restoration in a mouse model of crushed sciatic nerve. All the results suggest that ABPPk, as the key component of ABPP, can be used for peripheral nerve repair to yield better outcomes than ABPP. | Qiong Cheng Chunyi Jiang Caiping Wang Shu Yu Qi Zhang Xiaosong Gu Fei Ding | 2014 | Neural Regeneration Research2014，9，24： | 5 |
| 9 | Gene expression profiling of the rat sciatic nerve in early Wallerian degeneration after injury显示文摘Wallerian degeneration is an important area of research in modern neuroscience.A large number of genes are differentially regulated in the various stages of Wallerian degeneration,especially during the early response.In this study,we analyzed gene expression in early Wallerian degeneration of the distal nerve stump at 0,0.5,1,6,12 and 24 hours after rat sciatic nerve injury using gene chip microarrays.We screened for differentially-expressed genes and gene expression patterns.We examined the data for Gene Ontology,and explored the Kyoto Encyclopedia of Genes and Genomes Pathway.This allowed us to identify key regulatory factors and recurrent network motifs.We identified 1 546 differentially-expressed genes and 21 distinct patterns of gene expression in early Wallerian degeneration,and an enrichment of genes associated with the immune response,acute inflammation,apoptosis,cell adhesion,ion transport and the extracellular matrix.Kyoto Encyclopedia of Genes and Genomes pathway analysis revealed components involved in the Jak-STAT,ErbB,transforming growth factor-β,T cell receptor and calcium signaling pathways.Key factors included interleukin-6,interleukin-1,integrin,c-sarcoma,carcinoembryonic antigen-related cell adhesion molecules,chemokine(C-C motif) ligand,matrix metalloproteinase,BH3 interacting domain death agonist,baculoviral IAP repeat-containing 3 and Rac.The data were validated with real-time quantitative PCR.This study provides a global view of gene expression profiles in early Wallerian degeneration of the rat sciatic nerve.Our findings provide insight into the molecular mechanisms underlying early Wallerian degeneration,and the regulation of nerve degeneration and regeneration. | Dengbing Yao Meiyuan Li Dingding Shen Fei Ding Shibi Lu Qin Zhao Xiaosong Gu | 2012 | Neural Regeneration Research2012，7，17： | 5 |
| 10 | Qualitative and quantitative detection using eDNA technology: A case study of Fenneropenaeus chinensis in the Bohai Sea显示文摘Accurate knowledge of species distributions and population dynamics is the basis for conservation biology.However, for certain species with unique life histories and very small populations, monitoring species distributions and population dynamics is extremely difficult. Recently, newly developed eDNA technology has been widely used in species monitoring, biodiversity assessments, and biomass assessments. In this research, we studied Fenneropenaeus chinensis in the Bohai Sea. We used a 0.45 μm glass fiber filter combined with a vacuum to filter 2 L seawater samples for eDNA enrichment from 54 stations in June and 60 stations in August. A DNeasy Blood and Tissue kit was used to extract the eDNA. After DNA extraction, the specific primers and probes of the mitochondrial DNA COI gene of F. chinensis were designed. Real-time quantitative PCR was used to qualitatively and quantitatively analyze the eDNA in the Bohai Sea. The temporal and spatial distribution of F. chinensis was detected by the eDNA technique. The relationship between the eDNA copy number and trawl-netted F. chinensis biomass was explored. The applicability and sensitivity of eDNA technology in marine crustacean research were verified. The results showed that the mtDNA COI gene of F. chinensis was successfully amplified in the 54 water samples collected in June, and the detection rate reached 100%. Detections occurred in only 23 of the 60 stations sampled in August. F. chinensis had a detection rate of only 38%. The copy number of eDNA obtained by realtime PCR was fitted to the density of F. chinensis captured by bottom trawling. There was not a significant positive correlation between copy number and biomass. The results obtained through this research will provide a method and theoretical basis for the application of eDNA technology in marine crustacean research. | Miao Lia Xiujuan Shan Weiji Wang Xiaosong Ding Fangqun Dai Ding Lv Huanhuan Wu | 2020 | Aquaculture and Fisheries2020，5，3： | 4 |
| 11 | Achyranthes bidentata Blume extract promotes neuronal growth in cultured embryonic rat hippocampal neurons显示文摘We have prepared an aqueous extract of Achyranthes bidentata Blume, a commonly prescribed Chinese medicinal herb, and reported, in previous studies, that A. bidentata extract benefits nerve growth and prevents neuron apoptosis. In this study, we investigated the actions of A. bidentata extract on survival and growth of primarily cultured rat hippocampal neurons. The morphological observation revealed that neurite growth from hippocampal neurons was significantly enhanced by A. bidentata extract with similar effects to those induced by nerve growth factor (NGF), and the greatest neurite growth appeared on treatment with A. bidentata extract at 1 lg/ml for 24 h. DNA microarray analysis indicated that there were 25 upregulated genes and 47 downregulated genes exhibiting significantly differential expression in hippocampal neurons treated with A. bidentata extract at 1 lg/ml for 6 h when compared to those in untreated hippocampal neurons. Real-time quantitative RT-PCR and Western blot analysis demonstrated that the expression of growth-associated protein-43 in hippocampal neurons was upregulated at both mRNA and protein levels after treatment with A. bidentata extract, and the optimal dosage of the extract was also 1 lg/ml. These data confirm that A. bidentata extract could promote in vitro hippocampal neuronal growth in a dose-and time-dependent manner. | Xin Tang Yiren Chen Xiaosong Gu Fei Ding | 2009 | Progress in Natural Science:Materials International2009，19，5： | 4 |
| 12 | Single-cell atlas reveals a distinct immune profile fostered by T cell-B cell crosstalk in triple negative breast cancer显示文摘Background:Characterizing the unique immune microenvironment of each tumor is of great importance for better predicting prognosis and guiding cancer immunotherapy.However,the unique features of the immune microenvironment of triple negative breast cancer(TNBC)compared with other subtypes of breast cancer remain elusive.Therefore,we aimed to depict and compare the immune landscape among TNBC,human epidermal growth factor receptor 2-positive(HER2^(+))breast cancer,and luminal-like breast cancer.Methods:Single-cell RNA sequencing(scRNA-seq)was performed on CD45^(+)immune cells isolated from human normal breast tissues and primary breast tumors of various subtypes.By analyzing the scRNA-seq data,immune cell clusters were identified and their proportions as well as transcriptome features were compared among TNBC,human HER2^(+)breast cancer,and luminal-like breast cancer.Pseudotime and cell-cell communication analyses were also conducted to characterize the immune microenvironment.Results:ScRNA-seq data of 117,958 immune cells were obtained and 31 immune clusters were identified.A unique immunosuppressive microenvironment in TNBC was decoded as compared to that in HER2^(+)or luminal-like breast cancer,which was characterized by higher proportions of regulatory T cells(Tregs)and exhausted CD8+T cells and accompanied by more abundant plasma cells.Tregs and exhausted CD8+T cells in TNBC exhibited increased immunosuppression signature and dysfunctional scores.Pseudotime analyses showed that B cells tended to differentiate to plasma cells in TNBC.Cell-cell communication analyses indicated that these unique features are fostered by the diversified T cell-B cell crosstalk in TNBC.Based on the T cell-B cell crosstalk,a prognostic signaturewas established that could effectively predict the prognosis status for patients with TNBC.Additionally,it was found that TNBC had a higher proportion of cytotoxic natural killer(NK)cells,whereas HER2^(+)or luminal-like breast cancer lost this feature,suggesting thatHER2^(+)or luminal-like breast cancer,but not TNBC,may benefit from NK-based immunotherapy.Conclusions:This study identified a distinct immune feature fostered by T cell-B cell crosstalk in TNBC,which provides better prognostic information and effective therapeutic targets for breast cancer. | Shuning Ding Niu Qiao Qingchen Zhu Yiwei Tong Shengyue Wang Xiaosong Chen Qiang Tian Yichuan Xiao Kunwei Shen | 2023 | Cancer Communications2023，43，6： | 2 |
| 13 | BUB1B and circBUB1B_544aa aggravate multiple myeloma malignancy through evoking chromosomal instability显示文摘Multiple myeloma(MM)is an incurable plasma cell malignancy in the bone marrow characterized by chromosome instability(CIN),which contributes to the acquisition of heterogeneity,along with MM progression,drug resistance,and relapse.In this study,we elucidated that the expression of BUB1B increased strikingly in MM patients and was closely correlated with poor outcomes.Overexpression of BUB1B facilitated cellular proliferation and induced drug resistance in vitro and in vivo,while genetic targeting BUB1B abrogated this effect.Mechanistic studies unveiled that enforced expression of BUB1B evoked CIN resulting in MM poor outcomes mainly through phosphorylating CEP170.Interestingly,we discovered the existence of circBUB1B_544aa containing the kinase catalytic center of BUB1B,which was translated by a circular RNA of BUB1B.The circBUB1B_544aa elevated in MM peripheral blood samples was closely associated with MM poor outcomes and played a synergistic effect with BUB1B on evoking CIN.In addition,MM cells could secrete circBUB1B_544aa and interfere the MM microenvironmental cells in the same manner as BUB1B full-length protein.Intriguingly,BUB1B siRNA,targeting the kinase catalytic center of both BUB1B and circBUB1B_544aa,significantly inhibited MM malignancy in vitro and in vivo.Collectively,BUB1B and circBUB1B_544aa are promising prognostic and therapeutic targets of MM. | Xiaozhu Tang Mengjie guo Pinggang Ding Zhendong Deng Mengying Ke Yuxia Yuan Yanyan Zhou Zigen Lin Muxi Li Chunyan Gu Xiaosong Gu Ye Yang | 2021 | Signal Transduction and Targeted Therapy2021，6，11： | 2 |
| 14 | Thermodynamic Analysis on the Performance of Barocaloric Refrigeration Systems Using Neopentyl Glycol as the Refrigerant显示文摘Barocaloric refrigeration is regarded as one of the next-generation alternative refrigeration technology due to its environmental friendliness.In recent years,many researchers have been devoted to finding materials with colossal barocaloric effects,while neglecting the research on barocaloric refrigeration devices and thermodynamic cycles.Neopentyl glycol is regarded as one of the potential refrigerants for barocaloric refrigeration due to its giant isothermal entropy changes and relatively low operating pressure.To evaluate the performance of the barocaloric system using Neopentyl glycol,for the first time,this study establishes a thermodynamic cycle based on the metastable temperature-entropy diagram.The performance of the proposed system is investigated from the aspects of irreversibility,operating temperature range,and operating pressure,and optimized with finite-rate heat transfer.The guidance for the optimal design of the system is given by revealing the effect of the irreversibility in two isobaric processes.The results show that a COP of 8.8 can be achieved at a temperature span of 10 K when the system fully uses the phase transition region of Neopentyl glycol,while a COP of 3 can be achieved at a temperature span of 10 K when the system operates at room temperature.Furthermore,this study also shows that the system performance can be further improved through the modification of Neopentyl glycol,and some future development guidance is provided. | DAI Zhaofeng SHE Xiaohui WANG Chen DING Yulong ZHANG Xiaosong ZHAO Dongliang | 2023 | Journal of Thermal Science2023，32，3： | 2 |
| 15 | The promotion of peripheral nerve regeneration by chitooligosaccharides in the rat nerve crush injury model显示文摘 | Maorong Jiang Xiaoming Zhuge Yumin Yang Xiaosong Gu Fei Ding | 2009 | Neuroscience Letters2009，，3： | 1 |
| 16 | High hydrostatic pressure and thermal treatments for ready-to-eat wine-marinated shrimp: An evaluation of microbiological and physicochemical qualities显示文摘 | Junjie Yi Liang Zhang Guowei Ding Xiaosong Hu Xiaojun Liao Yan Zhang | 2013 | Innovative Food Science and Emerging Technologies2013，，： | 1 |
| 17 | S100 protein expression during induced Schwann cell-like cell differentiation of rat bone marrow mesenchymal cells in vitro显示文摘BACKGROUND: S100 protein can promote axonal growth. Therefore, transplantation of induced bone marrow-derived mesenchymal stem cells (MSCs) that can secrete S100 may provide a beneficial microenvironment for neural regeneration. OBJECTIVE: To explore the changes in S100 expression during rat MSCs differentiation into Schwann cells in vitro. DESIGN, TIME AND SETTING: This cytology experiment was performed at the Jiangsu Key Laboratory of Neuroregeneration, Nantong University in China, from January 2006 to May 2007. MATERIALS: The rabbit anti-S100 polyclonal antibody was purchased from Dako, Denmark; the mouse anti-rat S100 monoclonal antibody was purchased from Sigma, USA. METHODS: MSCs were cultured from adult Sprague-Dawley rat femur and tibia. Cell proliferation was determined by the MTT method and CD markers, and cell cycle was meas-ured by flow cytometry. MSCs were induced to differentiate into SC cells. SC cells were stained for S100 protein, glial fibrillary acidic protein, and low-affinity nerve growth factor receptor. S100 protein and mRNA levels were evaluated by flow cytometry, Western blot, and reverse transcription-polymerase chain reaction. MAIN OUTCOME MEASURES: S100 protein and mRNA expression. RESULTS: MSCs exhibited high amplification potential over eight passages. Prior to induction, the majority of MSCs were at the G0/G1 phase of the cell cycle. After induction, MSCs displayed morphology changes similar to Schwann cells. Moreover, induction increased S100 mRNA levels. Immunofluorescence showed that MSCs expressed S100 protein, glial fibrillary acidic protein, and low-affinity nerve growth factor receptor at 7 days of induction. Induction also increased S100 protein levels compared with untreated MSCs. CONCLUSION: MSCs are capable of differentiating into Schwann cells-like cells under conditional induction in vitro, with increasing S100 mRNA and protein expression. | Wenting Li Zenglu Xu Fei Ding Xiaosong Gu | 2010 | Neural Regeneration Research2010，5，3： | 1 |
| 18 | Protective effects of nerve regeneration factor and brain-derived neurotrophic factor on retinal ganglion cells in a rabbit model of acute hyper-intraocular pressure显示文摘BACKGROUND:Previous studies have shown that nerve regeneration factor (NRF) provides neuroprotective effects. However,the neuroprotective effects on retinal ganglion cells in an animal model of glaucoma remain uncertain. OBJECTIVE:To determine the neuroprotective effects of NRF on retinal ganglion cells in a rabbit model of acute hyper-intraocular pressure and to compare the effects on brain-derived neurotrophic factor (BDNF). DESIGN,TIME AND SETTING:A randomized,controlled,animal experiment was performed at Jiangsu Provincial Key Laboratory of Neural Regeneration from September 2006 to August 2007. MATERIALS:Sterone,a major component of NRF,was provided by the Key Laboratory of Neural Regeneration,Nantong University in China; BDNF was provided by BioDesign Inc.,USA. METHODS:A total of 24 healthy rabbits were randomly assigned to NRF,BDNF,and phosphate buffered saline groups,with 8 rabbits per group. The left eyes were considered normal controls,and acute hyper-intraocular pressure was induced in the right eyes via anterior chamber perfusion. The right camera vitrea bulbi was injected with 4.5 μg NRF,3.75 μg BDNF,or 5 μL 0.1 mol/L phosphate buffered saline,respectively. MAIN OUTCOME MEASURES:Retinal ganglion cells were reverse-labeled using horseradish peroxidase to quantify cell density at 2,4,and 6 mm from the optic disc edge. RESULTS:NRF increased the number of surviving retinal ganglion cells at the optic disc edge (P < 0.01 or P < 0.05). The density of surviving retinal ganglion cells decreased with increasing distance from the optic disc. The number of retinal ganglion cells in the BDNF group was similar to the NRF group (P > 0.05). At 2,4,and 6 mm away from the optic disc edge,there was no significant difference in retinal ganglion cell density between NRF and BDNF groups (P > 0.05). CONCLUSION:NRF provided protection to retinal ganglion cells in a rabbit model of acute hyper-intraocular pressure,i.e.,NRF enhanced the survival rate of retinal ganglion cells. The neuroprotective effect was similar to BDNF. | Zhengru Huang Huaijin Guan Fei Ding Xiaosong Gu | 2010 | Neural Regeneration Research2010，5，6： | 1 |
| 19 | Neurotrophic Actions of Bone Marrow Stromal Cells on Primary Culture of Dorsal Root Ganglion Tissues and Neurons显示文摘 | Yun Gu Jie Wang Fei Ding Nan Hu Yaxian Wang Xiaosong Gu | 2010 | Journal of Molecular Neuroscience2010，，3： | 1 |
| 20 | Bone marrow mesenchymal stem cells promote cell proliferation and neurotrophic function of Schwann cells in vitro and in vivo显示文摘 | Jie Wang Fei Ding Yun Gu Jie Liu Xiaosong Gu | 2009 | Brain Research2009，，： | 1 |
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