|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Millennial temperature reconstruction based on tree-ring widths of Qilian juniper from Wulan,Qinghai Province,China显示文摘The climate of the past 1000 years is an important context for evaluating the recent climate warming.However,there are few 1000-year-long climate reconstructions with annual resolution in the Qing-hai-Tibet Plateau.In this paper,a dendroclimatic analysis was conducted for the radial growth of Qilian juniper from the upper forest limit in Wulan,Qinghai Province.The results of correlation analysis between the tree-ring widths and the climate variables indicate that the growth of junipers at the upper forest limit is mainly limited by low temperatures of September,November and February of the pregrowth season,and July of the current growth season.There is no significant correlation between the tree-ring widths and precipitation.A mean temperature from the previous year's September to the current year's April was reconstructed for the Wulan area since A.D.1000.The reconstruction can explain 40.8% of the instrumental variance in the calibration period(1856―2002).The reconstruction shows that the 20th century is the warmest 100 years,and the 1990s is the warmest decade during the past 1000 years,while the coldest 100 years and decade occur at 1600―1699 and 1642―1651,respectively.The variations are verified well by the temperature reconstruction of the middle Qilian Mountain(QL) and the total organic carbon(TOC) in the Qinghai Lake sediments.The comparison of our reconstruction with the annual temperature reconstruction of extra-tropical Northern Hemisphere suggests that the climate of Wulan during the Medieval Warm Period is of obvious regional specialty,but there was a good response to the climate of hemispheric scales during the recent 400 years. | ZHU HaiFeng ZHENG YongHong SHAO XueMei LIU XiaoHong XU Yan LIANG ErYuan | 2008 | Chinese Science Bulletin2008，53，24： | 40 |
| 2 | Effects of dietary Clostridium butyricum supplementation on growth performance,intestinal development,and immune response of weaned piglets challenged with lipopolysaccharide显示文摘Background: Weanling pigs, with immature immune system and physiological function, usually experience postweaning diarrhea. This study determined the effects of dietary Clostridium butyricum supplementation on growth performance, diarrhea, and immunity of weaned pigs challenged with lipopolysaccharide(LPS).Methods: In Experiment(Exp.) 1,144 weaned piglets were weaned at 21 d and randomly assigned to six groups,with six replicates per group and four pigs per replicate, receiving a control diet(CON) or diet supplemented with antibiotics(AB) or C. butyricum(CB)(0.1%, 0.2%, 0.4%, or 0.8%), respectively. All diets in Exp. 1 were a highly digestible basal diet, with 3,000 mg/kg zinc oxide supplied in the first 2 wk only. In Exp. 2, 180 piglets were weaned at 21 d and randomly assigned to five groups, with six replicates per group and six pigs per replicate, receiving CON, AB, or CB(0.2%, 0.4%, or 0.6%) diets. The digestibility of diets was lower than those in Exp. 1, and did not include zinc oxide. At 36 d of Exp. 2, 12 piglets were selected from each of the CON and 0.4% CB groups, six piglets were intraperitoneally injected with LPS(50 μg/kg body weight) and the other six piglets with normal saline;animals were killed at 4 h after injection to collect blood, intestine, and digesta samples for biochemical analysis.Results: In Exp. 1, CB and AB diets had no effect on growth performance of piglets. In Exp. 2, 0.4% CB decreased feed-gain ratio(P < 0.1), diarrhea score(P < 0.05), and increased duodenal, jejunal, and ileal villus height and jejunal villus height/crypt depth(P < 0.05). The 0.4% CB decreased the plasma tumor necrosis factor(TNF) α(P < 0.05) but increased ileal mucosa IL-10 and TLR2 mRNA expression(P < 0.05). Furthermore, 0.4% CB altered the microbial profile, with Bacillus and Ruminococcaceae UGG-003 at genus level and Lactobacillus casei and Parasutterella secunda at species level were higher than CON in colonic content(P < 0.05).Conclusions: Dietary C. butyricum supplementation had positive effects on growth of weaned piglets with less digestible diets. There was a tendency to reduce the feed-gain ratio, which could reduce feed costs in pig production. Moreover, C. butyricum decreased post-weaning diarrhea by improving the intestinal morphology,intestinal microflora profile, and immune function. | Ling Chen Shuang Li Jie Zheng Wentao Li Xuemei Jiang Xilun Zhao Jian Li Lianqiang Che Yan Lin Shengyu Xu Bin Feng Zhengfeng Fang De Wu | 2018 | Journal of Animal Science and Biotechnology2018，9，4： | 26 |
| 3 | Comparative study of microRNA profiling in keloid fibroblast and annotation of differential expressed microRNAs显示文摘蟹状肿是由于皮损害的异常愈合成长的像肿瘤的皮疤,没有有效 treatment.The 分子的机制位于 \O 下面蟹状肿致病仍然大部分未知。在这研究,我们比较了表示在导出蟹状肿的成纤维细胞和正常成纤维细胞之间介绍的 microRNA (miRNA )( 包括胎儿、成年的真皮的成纤维细胞) 由 miRNA microarray 分析。我们发现在导出蟹状肿的成纤维细胞的 miRNA 侧面与在 normalfibroblasts 的那些是不同的。九 miRNAs 是表示其六在蟹状肿成纤维细胞(KF ) 是显著地起来调整的差别,包括 miR-152, miR-23b-3p, miR-31-5p, miR-320c, miR-30a-5p,和 hsv1-miR-H7,并且其三是调整 significantlydown 的包括 miR-4328, miR-145-5p,和 miR-143-3p。差别的功能的注解表示了他们在为疤创伤愈合重要的几条发信号的小径被充实的 miRNA targetsrevealed。在结论,我们 demonstratethat miRNA 表示侧面在 KF 被改变与相比可以为探索蟹状肿的致病在胎儿、成年的真皮的成纤维细胞,和 expressionprofile 提供有用线索。miRNAs 可能部分由影响相关的几条发信号的小径使创伤愈合留下疤痕贡献 etiologyof 蟹状肿。 | Chao Li Yuehong Bai Hua Liu Xuemei Zuo Haichang Yao Yiming Xu Manlin Cao | 2013 | Acta Biochimica et Biophysica Sinica2013，45，8： | 15 |
| 4 | A system dynamics approach for water resources policy analysis in arid land:a model for Manas River Basin显示文摘The Manas River Basin in Xinjiang Uygur autonomous region,similar to other arid regions,is facing water constraints which challenge decision-makers as to how to rationally allocate the available water resources to meet the demands from industries and natural ecosystems.Policies which integrate the supply and demand are needed to address the water stress issues.An object-oriented system dynamics model was developed to capture the interrelationships between water availability and increasing water demands from the growth of industries,agricultural production and the population through modeling the decision-making process of the water exploration explicitly,in which water stress is used as a major indicator.The model is composed of four sectors:1) natural surface and groundwater resources;2) water demand;3) the water exploitation process,including the decision to build reservoirs,canals and pumps;4) water stress to which political and social systems respond through increasing the supply,limiting the growth or improving the water use efficiency.The model was calibrated using data from 1949 to 2009 for population growth,irrigated land area,industry output,perceived water stress,groundwater resources availability and the drying-out process of Manas River;and simulations were carried out from 2010 to 2050 on an annual time step.The comparison of results from calibration and observation showed that the model corresponds to observed behavior,and the simulated values fit the observed data and trends accurately.Sensitivity analysis showed that the model is robust to changes in model parameters related to population growth,land reclamation,pumping capacity and capital contribution to industry development capacity.Six scenarios were designed to investigate the effectiveness of policy options in the area of reservoir relocation,urban water recycling,water demand control and groundwater pumping control.The simulation runs demonstrated that the technical solutions for improving water availability and water use efficiency are not sustainable.Acknowledging the carrying capacity of water resources and eliminating a growth-orientated value system are crucial for the sustainability of the Manas River Basin. | ShanShan DAI LanHai LI HongGang XU XiangLiang PAN XueMei LI | 2013 | Journal of Arid Land2013，5，1： | 14 |
| 5 | Moisture variations over the past millennium characterized by Qaidam Basin tree-ring δ^(18)O显示文摘High-resolution tree-ring δ18O chronologies covering the last millennium,although scarce,are essential in understanding patterns of climatic changes in the northeastern region of the Qinghai-Tibetan Plateau.For this study,a tree-ring δ18O chronology with a temporal resolution of 3-years was developed from the long-lived Qilian juniper(Sabina przewalskii Kom.),extending back in time to AD 991.This long δ18O chronology was significantly correlated with the yearly δ18O in tree rings during the common period from 1800 to 2006,and was an effective proxy for relative humidity during the growing season.A low-frequency moisture pattern signified the occurrence of a slight drought during the Medieval Climate Anomaly,a marked occurrence of a wet period during the Little Ice Age,and a trend in increasing moisture levels,although lower than average,alongside the Twentieth Century warming trend.Comparisons to other hydroclimatic reconstructions indicate that this tree-ring18O chronology serves as a reliable paleo-humidity proxy for the Qaidam Basin as well as documenting details of past humidity levels in the region. | WANG WenZhi LIU XiaoHong XU GuoBao SHAO XueMei QIN DaHe SUN WeiZhen AN WenLing ZENG XiaoMin | 2013 | Chinese Science Bulletin2013，58，32： | 14 |
| 6 | Humoral immune response to circulating SARS-CoV-2 variants elicited by inactivated and RBD-subunit vaccines显示文摘SARS-CoV-2 variants could induce immune escape by mutations on the receptor-binding domain(RBD)and N-terminal domain(NTD).Here we report the humoral immune response to circulating SARS-CoV-2 variants,such as 501Y.V2(B.1.351),of the plasma and neutralizing antibodies(NAbs)elicited by CoronaVac(inactivated vaccine),ZF2001(RBD-subunit vaccine)and natural infection.Among 86 potent NAbs identified by high-throughput single-cell VDJ sequencing of peripheral blood mononuclear cells from vaccinees and convalescents,near half anti-RBD NAbs showed major neutralization reductions against the K417N/E484K/N501Y mutation combination,with E484K being the dominant cause.VH3-53/VH3-66 recurrent antibodies respond differently to RBD variants,and K417N compromises the majority of neutralizing activity through reduced polar contacts with complementarity determining regions.In contrast,the 242–244 deletion(242–244Δ)would abolish most neutralization activity of anti-NTD NAbs by interrupting the conformation of NTD antigenic supersite,indicating a much less diversity of anti-NTD NAbs than anti-RBD NAbs.Plasma of convalescents and CoronaVac vaccinees displayed comparable neutralization reductions against pseudo-and authentic 501Y.V2 variants,mainly caused by E484K/N501Y and 242–244Δ,with the effects being additive.Importantly,RBD-subunit vaccinees exhibit markedly higher tolerance to 501Y.V2 than convalescents,since the elicited anti-RBD NAbs display a high diversity and are unaffected by NTD mutations.Moreover,an extended gap between the third and second doses of ZF2001 leads to better neutralizing activity and tolerance to 501Y.V2 than the standard three-dose administration.Together,these results suggest that the deployment of RBD-vaccines,through a third-dose boost,may be ideal for combating SARS-CoV-2 variants when necessary,especially for those carrying mutations that disrupt the NTD supersite. | Yunlong Cao Ayijiang Yisimayi Yali Bai Weijin Huang Xiaofeng Li Zhiying Zhang Tianjiao Yuan Ran An Jing Wang Tianhe Xiao Shuo Du Wenping Ma Liyang Song Yongzheng Li Xiang Li Weiliang Song Jiajing Wu Shuo Liu Xuemei Li Yonghong Zhang Bin Su Xianghua Guo Yangyang Wei Chuanping Gao Nana Zhang Yifei Zhang Yang Dou Xiaoyu Xu Rui Shi Bai Lu Ronghua Jin Yingmin Ma Chengfeng Qin Youchun Wang Yingmei Feng Junyu Xiao Xiaoliang Sunney Xie | 2021 | Cell Research2021，31，7： | 12 |
| 7 | Spatial-temporal dynamics of desert vegetation and its responses to climatic variations over the last three decades:a case study of Hexi region in Northwest China显示文摘Analysis of spatial-temporal variations of desert vegetation under the background of climate changes can provide references for ecological restoration in arid and semi-arid areas.In this study,we used the Global Inventory Modeling and Mapping Studies(GIMMS)NDVI data from 1982 to 2006 and Moderate Resolution Imaging Spectroradiometer(MODIS)NDVI data from 2000 to 2013 to reveal the dynamics of desert vegetation in Hexi region of Northwest China over the past three decades.We also used the annual temperature and precipitation data acquired from the Chinese meteorological stations to analyze the response of desert vegetation to climatic variations.The average value of NDVImax(the maximum NDVI during the growing season)for desert vegetation in Hexi region increased at the rate of 0.65×10–3/a(P<0.05)from 1982 to 2013,and the significant increases of NDVImax mainly appeared in the typical desert vegetation areas.Vegetation was significantly improved in the lower reaches of Shule and Shiyang river basins,and the weighted mean center of desert vegetation mainly shifted toward the lower reaches of the two basins.Almost 95.32% of the total desert vegetation area showed positive correlation between NDVImax and annual precipitation,indicating that precipitation is the key factor for desert vegetation growth in the entire study area.Moreover,the areas with non-significant positive correlation between NDVImax and annual precipitation mainly located in the lower reaches of Shiyang and Shule river basins,this may be due to human activities.Only 7.64% of the desert vegetation showed significant positive correlation between NDVImax and annual precipitation in the Shule River Basin(an extremely arid area),indicating that precipitation is not the most important factor for vegetation growth in this basin,and further studies are needed to investigate the mechanism for this phenomenon. | YANG Xuemei LIU Shizeng YANG Taibao XU Xianying KANG Caizhou TANG Jinnian WEI Huaidong Mihretab G GHEBREZGABHER LI Zhiqi | 2016 | Journal of Arid Land2016，8，4： | 10 |
| 8 | NEW PRODUCT DESIGN STRATEGIES WITH SUBSIDY POLICIES显示文摘工业开发能充实民族生活,但是它也引起环境污染,它是在政府,消费者,和公司之中的重要担心的一个区域。政府们提出一些环境政策,它由为绿产品开发提供更大的刺激激发工业。消费者们根据他们的环境了解和态度被分类进平常的二个组和绿市场片断。在这方面,公司需要重新考虑他们的主要产品设计策略。由在顾客偏爱,公司产品策略,和政府津贴政策之中调查相互作用,这份报纸为新产品设计策略论述一个理论模型。在如此的策略的详细理论分析以后,我们发现了那激发公司选择环境地友好的产品设计策略,政府们应该使有效津贴政策就职。在同时考虑环境问题和公司好处以后,我们设计了一条津贴政策。与如此的一条政策,公司能改变他们的主要产品设计策略并且开发绿、平常的产品,从而增加公司利润并且改进全部的环境质量。 | Xuemei ZHANG Xiaoyan XU Ping HE | 2012 | Journal of Systems Science and Systems Engineering2012，21，3： | 10 |
| 9 | Homologous recombination in human embryonic stem cells using CRISPR/Cas9 nickase and a long DNA donor template显示文摘 | Zhili Rong Shengyun Zhu Yang Xu Xuemei Fu | 2014 | Protein & Cell2014，5，4： | 9 |
| 10 | Crystal structure of cytotoxin protein suilysin from Streptococcus suis显示文摘Cholesterol-dependent cytolysins(CDC)are pore forming toxins.A prototype of the CDC family members is perfringolysin O(PFO),which directly binds to the cell membrane enriched in cholesterol,causing cell lysis.However,an exception of this general observation is intermedilysin(ILY)of Streptococcus intermedius,which requires human CD59 as a receptor in addition to cholesterol for its hemolytic activity.A possible explanation of this functional difference is the conformational variation between the C-terminal domains of the two toxins,particularly in the highly conserved undecapeptide termed tryptophan rich motif.Here,we present the crystal structure of suilysin,a CDC toxin from the infectious swine pathogen Streptococcus suis.Like PFO,suilysin does not require a host receptor for hemolytic activity;yet the crystal structure of suilysin exhibits a similar conformation in the tryptophan rich motif to ILY.This observation suggests that the current view of the structure-function relationship between CDC proteins and membrane association is far from complete. | Lingfeng Xu Bo Huang Huamao Du Xuejun CZhang Jianguo Xu Xuemei Li Zihe Rao | 2010 | Protein & Cell2010，1，1： | 9 |
| 11 | CAS9 is a genome mutator by directly disrupting DNA-PK dependent DNA repair pathway显示文摘With its high efficiency for site-specific genome editing and easy manipulation,the clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR associated protein 9(CAS9)system has become the most widely used gene editing technology in biomedical research.In addition,significant progress has been made for the clinical development of CRISPR/CAS9 based gene therapies of human diseases,several of which are entering clinical trials.Here we report that CAS9 protein can function as a genome mutator independent of any exogenous guide RNA(gRNA)in human cells,promoting genomic DNA double-stranded break(DSB)damage and genomic instability.CAS9 interacts with the KU86 subunit of the DNA-dependent protein kinase(DNA-PK)complex and disrupts the interaction between KU86 and its kinase subunit,leading to defective DNA-PK-dependent repair of DNA DSB damage via non-homologous end-joining(NHEJ)pathway.XCAS9 is a CAS9 variant with potentially higher fidelity and broader compatibility,and dCAS9 is a CAS9 variant without nuclease activity.We show that XCAS9 and dCAS9 also interact with KU86 and disrupt DNA DSB repair.Considering the critical roles of DNA-PK in maintaining genomic stability and the pleiotropic impact of DNA DSB damage responses on cellular proliferation and survival,our findings caution the interpretation of data involving CRISPR/CAS9-based gene editing and raise serious safety concerns of CRISPR/CAS9 system in clinical application. | Shuxiang Xu Jinchul Kim Qingshuang Tang Qu Chen Jingfeng Liu Yang Xu Xuemei Fu | 2020 | Protein & Cell2020，11，5： | 8 |
| 12 | Effects of dietary supplementation with essential oils and protease on growth performance,antioxidation,inflammation and intestinal function of weaned pigs显示文摘This experiment evaluated the impacts of essential oils(EO)and protease(PRO),independently or in combination,on growth performance,antioxidation,inflammation and intestinal function of weaned pigs.One hundred and sixty weaned pigs(21 d of age,BW of 6.74±0.20 kg)were randomly divided into 4 treatments with 8 replicate pens of 5 pigs per pen.Dietary treatments included the following:1)control diet(CON),2)CON with 300 mg/kg essential oils(EO),3)CON with 500 mg/kg protease(PRO),4)CON with 300 mg/kg essential oil and 500 mg/kg protease(EO + PRO).On d 8,one pig from each penwas selected for sampling.The remaining pigs were fed for an additional week and growth performance was monitored during this period.Dietary treatments had no marked effects(P>0.05)on the growth performance of pigs.However,pigs receiving EO diet had higher(P<0.05)serumglutathione peroxidase(GSH-Px)activity,and tended to decrease(P=0.063)serum concentration of tumor necrosis factor-a(TNF-a).In addition,pigs receiving EO diet had higher(P<0.05)abundances of phylum Actinobacteria,and genera Bifidobacterium,and lower(P<0.05)phylum Bacteroidetes and genera Alloprevotella in colonic digesta.Pigs receiving PRO diet decreased(P<0.05)the serum concentration of malondialdehyde(MDA)and diamine oxidase activity,increased(P<0.05)the villus height and the ratio of villus height to crypt depth in duodenum,increased sucrase activity in jejunal mucosa,and also increased the abundance of phylum Actinobacteria in colonic digesta.Furthermore,the synergistic effects of EO and PROwas observed(P<0.05)for pigs with decreasing serum TNF-a concentration and increasing serum GSH-Px activity.Collectively,the results indicated that dietary supplementation of EO and PRO had no significant effects on growth performance of weaned pigs.EO diet appeared to improve antioxidant activity and intestinal microbiota,while PRO diet improved intestinal morphology and digestive enzyme activity,and there was a synergistic effect of EO and PRO on reducing inflammatory parameters in weaned pigs. | Xie Peng Qiang Zhou Cheng Wu Jian Zhao Quan Tan Ying He Liang Hu Zhengfeng Fang Yan Lin Shengyu Xu Bin Feng Jian Li Yong Zhuo Chris Van Ginneken Xuemei Jiang De Wu Lianqiang Che | 2022 | Animal Nutrition2022，，2： | 8 |
| 13 | Standardization of Qilian Juniper ring-width series in the eastern margin of the Qaidam Basin显示文摘Standardization is one of the important procedures in dendroclimatology. We used abundant Qilian juniper (Sabina przewalskii Kom.) tree-ring samples from the eastern margin of the Qaidam Basin to develop a new standardization method, i.e. total growth curve (TGC). The samples that contained the complete pith and reached to the growing culmination around the 40th–60th year were used to fit TGC, and the generalized negative exponential function was used to fit the curve. Usually, most cores cannot reach the arboreal pith for some reasons and it is difficult to determine the arboreal cambial age. The empirical model of initial radial growth (IRG) was employed to estimate the number of rings missing from the pith by the same data and IRG model explained 90.9% of the variance. When developing the chronology, the cambial ages of cores that contained the complete pith were regarded as beginning from the first year and others were determined by the numbers of missing and included rings in the core. Standardization was accomplished by dividing each tree-ring series by corresponding TGC. The chronologies developed by TGC can retain more low-frequency variational infor- mation and TGC helps to develop more reliable tree-ring width chronology. | XU Yan SHAO Xuemei | 2007 | Journal of Geographical Sciences2007，17，2： | 8 |
| 14 | The Glutamatergic Postrhinal Cortex–Ventrolateral Orbitofrontal Cortex Pathway Regulates Spatial Memory Retrieval显示文摘A deficit in spatial memory has been taken as an early predictor of Alzheimer’s disease(AD) or mild cognitive impairment(MCI). The uncinate fasciculus(UF) is a long-range white-matter tract that connects the anterior temporal lobe with the orbitofrontal cortex(OFC)in primates. Previous studies have shown that the UF impairment associated with spatial memory deficits may be an important pathological change in aging and AD, but its exact role in spatial memory is not well understood. The pathway arising from the postrhinal cortex(POR) and projecting to the ventrolateral orbitofrontal cortex(vlOFC)performs most of the functions of the UF in rodents.Although the literature suggests an association between spatial memory and the regions connected by the POR–vlOFC pathway, the function of the pathway in spatialmemory is relatively unknown. To further illuminate the function of the UF in spatial memory, we dissected the POR–vlOFC pathway in mice. We determined that the POR–vlOFC pathway is a glutamatergic structure, and that glutamatergic neurons in the POR regulate spatial memory retrieval. We also demonstrated that the POR–vlOFC pathway specifically transmits spatial information to participate in memory retrieval. These findings provide a deeper understanding of UF function and dysfunction related to disorders of memory, as in MCI and AD. | Xinyang Qi Zhanhong Jeff Du Lin Zhu Xuemei Liu Hua Xu Zheng Zhou Cheng Zhong Shijiang Li Liping Wang Zhijun Zhang | 2019 | Neuroscience Bulletin2019，35，3： | 7 |
| 15 | ADVANCE SELLING IN THE PRESENCE OF PRODUCT DIFFUSION EFFECT显示文摘产品散开指以后的需求依赖于早出售的现象。调查卖策略的公司进展怎么被产品散开的效果影响,我们考虑一个独占者卖主在在二个时期上近视、战略的消费者包括的一个市场卖一个时髦产品(即,卖季节的进展和常规卖季节) 。为我们发现那的线性产品散开效果,当产品散开的效果是积极的时,卖主可以设置一个极其高的进展售价劝诱战略消费者在常规卖季节购买,它是违反直觉的。而且,为卖主的最佳的获得数量可以在战略消费者的数量在产品散开和减少的否定效果增加。当我们扩大我们的模型认为凹面二次时产品散开效果然而,最佳的获得数量是在战略消费者的数量的凹面。数字研究进一步被介绍讨论经理的卓见。 | Xiaoyan Xu Xuemei Li Yiwen Bian Yanhong Sun | 2017 | Journal of Systems Science and Systems Engineering2017，26，1： | 7 |
| 16 | Cloning and Characterization of a New κ-Carrageenase Gene from Marine Bacterium Pseudoalteromonas sp. QY203显示文摘κ-carrageenan oligosaccharides exhibit various biological activities. Enzymatic degradation by κ-carrageenase is safe and controllable. Therefore, κ-carrageenases have captured more and more attentions. In this study, a κ-carrageenase encoding gene, cgk X, was cloned from Pseudoalteromonas sp. QY203 with degenerate and inverse PCR. It comprised an ORF of 1194 bp in length, encoding a protein with 397 amino acid residues. Cgk X is a new member of glycoside hydrolase family 16. The deduced amino acid sequence shared a high similarity with Cgk X of Pseudoalteromonas κ-carrageenase; however, the recombinant Cgk X showed different biochemical characteristics. The recombinant enzyme was most active at p H 7.0 and 55℃ in the presence of 300 mmol L^(^(-1))Na Cl. It was stable in a broad range of acidity ranging from p H 3.0 to p H 10.0 when temperature was below 40℃. More than 80% of its activity was maintained after being incubated at p H 3.6–10.0 and 4℃ for 24 h. Cgk X retained more than 90% of activity after being incubated at 40℃ for 1 h. EDTA and SDS(1 mmol L^(-1)) did not inhibit its activity. Cgk X hydrolyzed κ-carrageenan into disaccharide and tetrasaccharide as an endo-cleaver. All these characteristics demonstrated that Cgk X is applicable to both κ-carrageenan oligosaccharide production and κ-carrageenase structure-function research. | XU Xiaoyan LI Shangyong YANG Xuemei YU Wengong HAN Feng | 2015 | Journal of Ocean University of China2015，14，6： | 5 |
| 17 | Selectivity regulation of CO2 electroreduction through contact interface engineering on superwetting Cu nanoarray electrodes显示文摘Electrocatalytic CO2 reduction is a promising way to mitigate the urgent energy and environmental issues, but how to increase the selectivity for desired product among multiple competi ng reaction pathways remains a bottle neck. Here, we demonstrate that engineering the gas-liquid-solid contact interface on the electrode surface could tailor the selectivity of CO2 reducti on and meanwhile suppress H2 production through regulated reacti on kin etics. Specifically, polytetrafl uoroethyle ne (PTFE) was utilized to modify a Cu nano array electrode as an example, which is able to cha nge the electrode surface from aerophobic to aerophilic state. The en riched nano-tunnels of the Cu nano array electrode can facilitate CO2 transportation and pin gaseous products on the electrode surface. The latter is believed to be the reason that boosts the Faradaic efficie ncy of liquid products by 67% and limits the H2 producti on to less than half of before. This in terface engin eeri ng strategy also lowered H2O (proton) affinity, therefore promoting CO and HCOOH production. Engineering the electrode contact interface controls the reaction kinetics and the selectivity of products, which should be inspiring for other electrochemical reactions. | Zhao Cai Yusheng Zhang Yuxin Zhao Yueshen Wu Wenwen Xu Xuemei Wen Yang Zhong Ying Zhang Wen Liu Hailiang Wang Yun Kuang Xiaoming Sun | 2019 | Nano Research2019，12，2： | 5 |
| 18 | Tree ring-dated fluctuation history of Midui glacier since the Little Ice Age in the southeastern Tibetan Plateau显示文摘Fluctuation history of Midui glacier in the southeastern Tibet since the Little Ice Age(LIA) was reconstructed by the dating of lateral and terminal moraines using tree rings.Four conversions of glacier advance/stabilization to retreat were identified at around 1767,1875,1924 and 1964.The glacier reached its LIA maximum position at 1767.The fluctuations are consistent with those of other glaciers from the Tibetan Plateau,the Rockies and the Alps,suggesting high spatial coherency of glacier fluctuations in the Northern Hemisphere.Comparison with the summer temperature reconstruction in the southeastern Tibetan Plateau indicated that the Midui glacier fluctuation may be related to temperature variation on the centennial timescale.On the decadal scale,the fluctuation could correspond to cold/warm variation with an 8-year lag on average. | XU Peng ZHU HaiFeng SHAO XueMei YIN ZhiYong | 2012 | Science China Earth Sciences2012，55，4： | 5 |
| 19 | An Anomalous Behavior Detection Model in Cloud Computing显示文摘This paper proposes an anomalous behavior detection model based on cloud computing. Virtual Machines(VMs) are one of the key components of cloud Infrastructure as a Service(Iaa S). The security of such VMs is critical to Iaa S security. Many studies have been done on cloud computing security issues, but research into VM security issues, especially regarding VM network traffic anomalous behavior detection, remains inadequate.More and more studies show that communication among internal nodes exhibits complex patterns. Communication among VMs in cloud computing is invisible. Researchers find such issues challenging, and few solutions have been proposed—leaving cloud computing vulnerable to network attacks. This paper proposes a model that uses Software-Defined Networks(SDN) to implement traffic redirection. Our model can capture inter-VM traffic, detect known and unknown anomalous network behaviors, adopt hybrid techniques to analyze VM network behaviors, and control network systems. The experimental results indicate that the effectiveness of our approach is greater than 90%, and prove the feasibility of the model. | Xiaoming Ye Xingshu Chen Haizhou Wang Xuemei Zeng Guolin Shao Xueyuan Yin Chun Xu | 2016 | Tsinghua Science and Technology2016，21，3： | 5 |
| 20 | Piezoluminescent devices by designing array structures显示文摘Mechanoluminescence has attracted increasing attentions because it can convert the kinetic energy during human daily motions into light to be used in sensors and displays. However, its practical applications are still hindered by the weak brightness and limited color while under large forces. Herein, we developed novel piezoluminescent devices(PLDs) which could effectively emit visible light under low pressing forces through the stress-concentration and enhancing deformation on the basis of carefully-designed array structures. The emitting colors were also tunable by using bilayer luminescent film under different pressures. This work not only provides a new strategy to effectively harvest mechanical energy into light,but also presents a scalable, low-cost and color-tunable PLD which shows great potentials in various applications such as luminescent floors, shoes and stress-activated displays. | Luke Bao Xiaojie Xu Yong Zuo Jing Zhang Fei Liu Yifan Yang Fan Xu Xuemei Sun Huisheng Peng | 2019 | Science Bulletin2019，64，3： | 5 |
您所在的机构暂未开通此部分的功能，请联系管理员