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| 1 | A complete sequence and comparative analysis of a SARS-associated virus(Isolate BJ01)显示文摘The genome sequence of the Severe Acute Respiratory Syndrome (SARS)-associated virus provides essential information for the identification of pathogen(s), exploration of etiology and evolution, interpretation of transmission and pathogenesis, development of diagnostics, prevention by future vaccination, and treatment by developing new drugs. We report the complete genome sequence and comparative analysis of an isolate (BJ01) of the coronavirus that has been recognized as a pathogen for SARS. The genome is 29725 nt in size and has 11 ORFs (Open Reading Frames). It is composed of a stable region encoding an RNA-dependent RNA polymerase (composed of 2 ORFs) and a variable region representing 4 CDSs (coding sequences) for viral structural genes (the S, E, M, N proteins) and 5 PUPs (putative uncharacterized proteins). Its gene order is identical to that of other known coronaviruses. The sequence alignment with all known RNA viruses places this virus as a member in the family of Coronaviridae. Thirty putative substitutions have been identified by comparative analysis of the 5 SARS- associated virus genome sequences in GenBank. Fifteen of them lead to possible amino acid changes (non-synonymous mutations) in the proteins. Three amino acid changes, with predicted alteration of physical and chemical features, have been detected in the S protein that is postulated to beinvolved in the immunoreactions between the virus and its host. Two amino acid changes have been detected in the Mprotein, which could be related to viral envelope formation. Phylogenetic analysis suggests the possibility of non-human origin of the SARS-associated viruses but provides noevidence that they are man-made. Further efforts should focus on identifying the etiology of the SARS-associated virus and ruling out conclusively the existence of otherpossible SARS-related pathogen(s). | QIN E'de ZHU Qingyu YU Man FAN Baochang CHANG Guohui SI Bingyin YANG Bao PENG Wenming JIANG Tao LIU Bohua DENG Yongqiang LIU Hong ZHANG Yu WANG Cui LI Yuquan GAN Yonghua LI Xiaoyu L Fushuang TAN Gang CAO Wuchun, YANG Ruifu Institute of Microbiology and Epidemiology, Chinese Academy of Military Medical Sciences, Beijing 100071, China WANG Jian, LI Wei, XU Zuyuan, LI Yan, WU Qingfa, LIN Wei, CHEN Weijun, TANG Lin, DENG Yajun, HAN Yujun, LI Changfeng, LEI Meng, LI Guoqing, LI Wenjie, L Hong, SHI Jianping, TONG Zongzhong, ZHANG Feng, LI Songgang, LIU Bin, LIU Siqi, DONG Wei, WANG Jun, Gane K-S Wong, YU Jun & YANG Huanming* Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300 National Center for Genome Information, Beijing 101300, China | 2003 | Chinese Science Bulletin2003，48，10： | 121 |
| 2 | Cobalt single atom site catalysts with ultrahigh metal loading for enhanced aerobic oxidation of ethylbenzene显示文摘The oxidation of hydrocarbons to produce high value-added compounds(ketones or alcohols)using oxygen in air as the only oxidant is an efficient synthetic strategy from both environmental and economic views.Herein,we successfully synthesized cobalt single atom site catalysts(Co SACs)with high metal loading of 23.58 wt.%supported on carbon nitride(CN),which showed excellent catalytic properties for oxidation of ethylbenzene in air.Moreover,Co SACs show a much higher turn-over frequency(19.6 h^(−1))than other reported non-noble catalysts under the same condition.Comparatively,the as-obtained nanosized or homogenous Co catalysts are inert to this reaction.Co SACs also exhibit high selectivity(97%)and stability(unchanged after five runs)in this reaction.DFT calculations reveal that Co SACs show a low energy barrier in the first elementary step and a high resistance to water,which result in the robust catalytic performance for this reaction. | Yu Xiong Wenming Sun Yunhu Han Pingyu Xin Xusheng Zheng Wensheng Yan Juncai Dong Jian Zhang Dingsheng Wang Yadong Li | 2021 | Nano Research2021，14，7： | 20 |
| 3 | Progress in the Study of False Smut Disease in Rice显示文摘 | Xiaoyi Guo Yan Li Jing Fan Liang Li Fu Huang Wenming Wang | 2012 | Journal of Agricultural Science and Technology(A)2012，2，11： | 15 |
| 4 | The Qitai radio telescope显示文摘This study presents a general outline of the Qitai radio telescope(QTT)project.Qitai,the site of the telescope,is a county of Xinjiang Uygur Autonomous Region of China,located in the east Tianshan Mountains at an elevation of about 1800 m.The QTT is a fully steerable,Gregorian-type telescope with a standard parabolic main reflector of 110 m diameter.The QTT has adopted an umbrella support,homology-symmetric lightweight design.The main reflector is active so that the deformation caused by gravity can be corrected.The structural design aims to ultimately allow high-sensitivity observations from 150 MHz up to115 GHz.To satisfy the requirements for early scientific goals,the QTTwill be equipped with ultra-wideband receivers and large field-of-view multi-beam receivers.A multi-function signal-processing system based on RFSo C and GPU processor chips will be developed.These will enable the QTT to operate in pulsar,spectral line,continuum and Very Long Baseline Interferometer(VLBI)observing modes.Electromagnetic compatibility(EMC)and radio frequency interference(RFI)control techniques are adopted throughout the system design.The QTT will form a world-class observational platform for the detection of lowfrequency(nano Hertz)gravitational waves through pulsar timing array(PTA)techniques,pulsar surveys,the discovery of binary black-hole systems,and exploring dark matter and the origin of life in the universe.The QTT will also play an important role in improving the Chinese and international VLBI networks,allowing high-sensitivity and high-resolution observations of the nuclei of distant galaxies and gravitational lensing systems.Deep astrometric observations will also contribute to improving the accuracy of the celestial reference frame.Potentially,the QTT will be able to support future space activities such as planetary exploration in the solar system and to contribute to the search for extraterrestrial intelligence. | Na Wang Qian Xu Jun Ma Zhiyong Liu Qi Liu Hailong Zhang Xin Pei Maozheng Chen Richard N.Manchester Kejia Lee Xingwu Zheng Hans J.Kärcher Wulin Zhao Hongwei Li Dongwei Li Martin Süss Matthias Reichert Zhongyi Zhu Congsi Wang Mingshuai Li Rui Li Ning Li Guljaina Kazezkhan Wenming Yan Gang Wu Lang Cui Ming Zhang Haitao Li | 2023 | Science China(Physics,Mechanics & Astronomy)2023，66，8： | 14 |
| 5 | Bio-inspired polygonal skeleton structure for vibration isolation:Design,modelling,and experiment显示文摘Inspired by the safe landing of a cat falling from a high altitude,a bio-inspired polygonal skeleton(BIPS) structure is proposed,and its nonlinear characteristics are systematically studied to explore its potential application in the suppression of vibration. The polygon is formed by the skeleton structure of the cat’s entire body and the ground. The BIPS system consists of two symmetrical bionic legs with three robs(as skeleton) and four horizontal springs(as muscle). Two bionic legs are connected through the bearing platform(as spine),which could adjust the distance between the two bionic legs. A theoretical model is developed to characterize its stiffness nonlinearity through geometrical and mechanical analysis. Parameter analysis reveals that the BIPS structure has diverse stiffness,including nonlinear positive stiffness and negative stiffness. By imitating adjustment of leg posture and telescopic function of the spine(control the distance between legs),these flexible stiffness properties can be adjusted by structure parameters. In addition,the load capacity and working range can also be designed by the length of the bars,the initial angle,the mounting position,and the spring stiffness. The experimental setup is established,and the vibration isolation performance under various excitation is tested. The experimental results verify the accuracy of the dynamic model and also show that the proposed BIPS structure can suppress the vibration effectively under a variety of excitations. These peculiarities may provide potential possibility of an innovative approach to passive vibration control and isolation. | YAN Ge WANG Sen ZOU HongXiang ZHAO LinChuan GAO QiuHua ZHANG WenMing | 2020 | Science China(Technological Sciences)2020，63，12： | 5 |
| 6 | Design,modeling and experimental investigation of a magnetically modulated rotational energy harvester for low frequency and irregular vibration显示文摘Vibration energy harvesting is a promising approach for sustainable energy generation from ambience to meet the development of self-powered systems.Here,we propose a novel compact non-resonant magnetically modulated rotational energy harvester(MMR-EH)for low frequency and irregular vibration.Through the rational arrangement of multiple magnetic fields in space,a ring route with low potential energy is established.A movable magnet can be non-contact modulated by the magnetic force to move along the ring route under irregular vibration,which is instrumental in electromechanical energy conversion.A dynamic model of the MMR-EH is developed based on the energy method and verified experimentally.The effects of key parameters on the magnetically modulated route are analysed.The simulation and experimental results demonstrate that the MMR-EH can effectively harvest the energy from ultra-low frequency(3 Hz)and irregular vibration.At a reciprocating vibration frequency of 10 Hz and an amplitude of 20 mm,the harvester can produce an average power of 0.29 mW. | ZHAO LinChuan ZOU HongXiang GAO QiuHua YAN Ge WU ZhiYuan LIU FengRui WEI KeXiang YANG Bin ZHANG WenMing | 2020 | Science China(Technological Sciences)2020，63，10： | 5 |
| 7 | eGPS 1.0: comprehensive software for multi-omic and evolutionary analyses显示文摘It has become increasingly challenging for researchers to analyze the exponentially expanding amount of multi-omic data.Here,we describe multi-functional software named evolutionary GenotypePhenotype Systems(eGPS)that enables users to perform comprehensive multiomic and evolutionary analyses.The eGPS combines the power of cloud computing and the advantage of desktop application,has a user-friendly graphic interface and is highly interactive. | Dalang Yu Lili Dong Fangqi Yan Hailong Mu Bixia Tang Xiao Yang Tao Zeng Qing Zhou Feng Gao Zhonghuang Wang Ziqian Hao Hongen Kang Yi Zheng Hongwei Huang Yuzhang Wei Wei Pan Yaochen Xu Junwei Zhu Shilei Zhao Ciran Wang Pengyu Wang Long Dai Mushan Li Li Lan Yiwei Wang Hua Chen Yi-Xue Li Yun-Xin Fu Zhen Shao Yiming Bao Fangqing Zhao Luo-Nan Chen Guo-Qing Zhang Wenming Zhao Haipeng Li | 2019 | National Science Review2019，6，5： | 4 |
| 8 | Chronic acalculous cholecystitis:correlation of clinical assessment,laboratory data and final histopathology显示文摘Objective:To summarize and analyze the clinical and histopathological features of chronic acalculous cholecystitis (CAC) and to investigate the diagnosis and surgical treatment of chronic acalculous cholecystit.Methods:The study subjects were 39 patients with chronic biliary symptoms but no evidence of stones in the gallbladder by B ultrasonography and magnetic resonance cholopancreatography (MRCP) (CAC group).The CCC group consisted of 66 patients taken randomly from concurrent all patients of chronic calculous cholecystitis (CCC).All patients accepted fibergastroscopy,B ultrasonography,MRCP,laboratory examination preoperatively.We retrospectively analyzed the clinical features,B ultrasonography and MRCP findings,histopathological results and clinical outcomes between the two groups.Results:All the 39 patients were diagnosed by clinical symptoms,B ultrasonography,fatty meal gallbladder contractability studies under ultrasound,fibergastroscopy and magnetic resonance cholangiopancreatography (MRCP),what's more,they were pathologically verified postoperatively.In all patients,there was a complete absence of gallbladder wall contractability.Mucosa epithelial defect was found in 21 patients in CAC group (53.8%) and 16 patients in CCC group (24.2%) respectively (P<0.005).Thickened arteriole wall was found in 29 patients in CAC group (74.4%) and none patient in CCC group (P<0.0001).Thickened gallbladder wall (4 mm or more in thickness) was found in 33 patients in CAC group (84.6%) and 28 patients in CCC group (42.4%) respectively (P<0.005).Bile stasis was found in 23 patients in CAC group (59.0%) and 14 patients in CCC group (21.2%) respectively by ultrasonography preoperatively and confirmed in operation (P<0.005).The outcomes of cholecystectomy,expressed as total or near total relief,was similar in the two groups.No statistically significant differences were observed between patients with CAC (90%) and CCC (80%),the P-value >0.05.Conclusion:Chronic acalculous cholecystitis could be diagnosed by symptoms,ultrasound,fatty meal gallbladder contractability studies under untrasoundand MRCP.The optimal treatment of chronic acalculous cholecystitis characterized by thickened arteriole wall and mucosa epithelial defect is cholecystectomy. | Yan Jianjun Zhu Qian Shen Jun Zhou Feiguo Huang Liang Liu Caifeng Zhang Xianghua Cong Wenming Yan Yiqun | 2012 | Journal of Medical Colleges of PLA(China)2012，27，2： | 4 |
| 9 | The Arabidopsis CROWDED NUCLEI genes regulate seed germination by modulating degradation of ABI5 protein显示文摘In Arabidopsis, the phytohormone abscisic acid(ABA)plays a vital role in inhibiting seed germination and in postgermination seedling establishment. In the ABA signaling pathway, ABI5, a basic Leu zipper transcription factor, has important functions in the regulation of seed germination. ABI5 protein localizes in nuclear bodies, along with AFP, COP1, and SIZ1,and was degraded through the 26 S proteasome pathway.However, the mechanisms of ABI5 nuclear body formation and ABI5 protein degradation remain obscure. In this study, we found that the Arabidopsis CROWDED NUCLEI(CRWN) proteins,predicted nuclear matrix proteins essential for maintenance of nuclear morphology, also participate in ABA-controlled seed germination by regulating the degradation of ABI5 protein. During seed germination, the crwn mutants are hypersensitive to ABA and have higher levels of ABI5 protein compared to wild type.Genetic analysis suggested that CRWNs act upstream of ABI5. The observation that CRWN3 colocalizes with ABI5 in nuclear bodiesindicates that CRWNs might participate in ABI5 protein degrada tion in nuclear bodies. Moreover, we revealed that the extreme C-terminal of CRWN3 protein is necessary for its function in the response to ABA in germination. Our results suggested importan roles of CRWNs in ABI5 nuclear body organization and ABI5 protein degradation during seed germination. | Wenming Zhao Chunmei Guan Jian Feng Yan Liang Ni Zhan Jianru Zuo Bo Ren | 2016 | Journal of Integrative Plant Biology2016，58，7： | 3 |
| 10 | Structural variation during dog domestication: insights from gray wolf and dhole genomes显示文摘Several processes like phenotypic evolution, disease susceptibility and environmental adaptations, which fashion the domestication of animals, are largely attributable to structural variations(SVs) in the genome.Here, we present high-quality draft genomes of the gray wolf(Canis lupus) and dhole(Cuon alpinus) with scaffold N50 of 6.04 Mb and 3.96 Mb, respectively. Sequence alignment comprising genomes of three canid species reveals SVs specific to the dog, particularly 16 315 insertions, 2565 deletions, 443 repeats, 16 inversions and 15 translocations. Functional annotation of the dog SVs associated with genes indicates their enrichments in energy metabolisms, neurological processes and immune systems. Interestingly, we identify and verify at population level an insertion fully covering a copy of the AKR1 B1(Aldo-Keto Reductase Family 1 Member B) transcript. Transcriptome analysis reveals a high level of expression of the new AKR1 B1 copy in the small intestine and liver, implying an increase in de novo fatty acid synthesis and antioxidant ability in dog compared to gray wolf, likely in response to dietary shifts during the agricultural revolution. For the first time, we report a comprehensive analysis of the evolutionary dynamics of SVs during the domestication step of dogs. Our findings demonstrate that retroposition can birth new genes to facilitate domestication, and affirm the importance of large-scale genomic variants in domestication studies. | Guo-Dong Wang Xiu-Juan Shao Bing Bai Junlong Wang Xiaobo Wang Xue Cao Yan-Hu Liu Xuan Wang Ting-Ting Yin Shao-Jie Zhang Yan Lu Zechong Wang Lu Wang Wenming Zhao Bing Zhang Jue Ruan Ya-Ping Zhang | 2019 | National Science Review2019，6，1： | 3 |
| 11 | Magnetic levitation using diamagnetism:Mechanism,applications and prospects显示文摘As a typical contact-free manipulation technique that removes friction and contamination risk,levitation has gradually become a preferred candidate for various applications.Magnetic levitation using diamagnetism,beyond Earnshaw’s theorem,is a kind of passive stable levitation that can be achieved at normal temperatures with no energy input.Appealingly,most seemingly nonmagnetic materials can be levitated in a magnetic field and can stabilize free levitation of magnetic materials.This review focuses on the fundamental principles of magnetic levitation using diamagnetism,with emphasis on its burgeoning applications.The theoretical basis associated with the magnetic levitation using diamagnetism is discussed by elucidating the characteristics of diamagnetic materials,and the key levitation mechanisms are clarified.Afterwards,state-of-the-art applications in various aspects,including sensing and measurement,actuating and micromanipulation,energy harvesting and magnetic gravity compensation,are summarized and compared.Finally,the review concludes with a brief outlook on future perspectives. | GAO QiuHua YAN Han ZOU HongXiang LI WenBo PENG ZhiKe MENG Guang ZHANG WenMing | 2021 | Science China(Technological Sciences)2021，64，1： | 3 |
| 12 | Rapid microwave-assisted synthesis of graphene nanosheet/Co 3 O 4 composite for supercapacitors显示文摘 | Jun Yan Tong Wei Wenming Qiao Bo Shao Qiankun Zhao Lijun Zhang Zhuangjun Fan | 2010 | Electrochimica Acta2010，，： | 2 |
| 13 | Efficient edge-preserving algorithm for color contrast enhancement with application to color image segmentation 显示文摘 | CHUNG Kuoliang YANG Weijen YAN Wenming | 2008 | Visual Image(S1047-3203)2008，19，5： | 1 |
| 14 | Rapid mi- crowave-assisted synthesis of graphene nanosheet/Co304 composite for supercapacitors 显示文摘 | YAN Jun WEI Tong QIAO Wenming | 2010 | Electrochimica ACTA2010，55，23： | 1 |
| 15 | Micron-to nano-pore characteristics in the shale of Longmaxi Formation,southeast Sichuan Basin显示文摘For shale of Lower Silurian Longmaxi Formation in Chongqing,southeast Sichuan Basin,characteristics of micro-nano pores in marine shale reservoirs were well studies by means of Field-Emission Scanning Electron Microscope and Low-temperature Low-pressure Adsorption Experiment of CO_(2)and N_(2).Results showed that six types of pore were developed in the shale of Longmaxi Formation,i.e.,organic pores,intergranular pores,intragranular pores,intercrystalline pores,dissolution pores and microfractures,among which the organic pores and intragranular pores in interlayers of clay minerals were most developed,and a plenty of dissolution pores were also well developed because of high thermal evolution degree.BET specific surface area of the shale in Longmaxi Formation ranged from 3.5 to 18.1 m^(2)/g,BJH total pore volume was from 0.00234 to 0.01338 cm^(3)/g,DA specific surface area of micropores vaired from 1.3 to 7.3 m^(2)/g,and DA pore volume ranged from 0.00052 to 0.00273 cm^(3)/g.The specific surface area of micropores in the shale accounted for 23.1%-80.2%of total specific surface area with an average of 50.3%,and the pore volume of micropores accounted for 12.1%-48.5%of total pore volume with an average of 32.3%.Micropore was the main storage space in shale reservoir for methane adsorption,that because capacities of specific surface area provided by micropores were considerably greater than those provided by mesopores and macropores.Pore size distribution of the shale was complex,and multiple different peaks occurred in the pore size curves,showing two or three peaks in the range from 0 to 100 nm and four peaks occasionally.TOC had a good linear relationship with pore structure parameters of micropores,mesopores t macropores and total pores in the shale,indicating that TOC was the most important control factor for micron-to nano-pore structure in the shale.After normalization of pore structure parameters to TOC,the pore structure parameters of total pores and mesopores t macropores,had positive linear relationships with content of clay minerals but negative linear relationships with content of brittle minerals,indicating that clay minerals and brittle minerals mainly controlled development of mesopores and macropores in the shale. | Wenming Ji Yan Song Zhenxue Jiang Mianmo Meng Qingxin Liu Fenglin Gao | 2017 | Petroleum Research2017，2，2： | 1 |
| 16 | Nonlinear characterization and performance optimization for broadband bistable energy harvester显示文摘As two crucial indicators of bistable energy harvesting performance,band width and power amplitude are simultaneously investigated for obtaining the synergy effect.Toward this goal,a nonlinear electromechanical-coupled distributed-parameter model of the bistable piezoelectric energy harvester is established.Based on the electromechanical decoupled method,approximate higher-order analytical solutions of the beam displacement,harvested power and effective bandwidth are derived.The cubic-function discriminant of the analytical solution is introduced to determine the nonlinear excitation-frequency boundaries of multiple solutions and power peak.The stability of the multiple solutions is analyzed through Jacobi matrix of the modulation equation.Superharmonic resonance is notified.Upward and downward sweep experiments and numerical solutions of time history curves,phase portraits and power spectra confirm the analytical findings.To realize optimized broadband energy harvesting,the parametric study on the coefficients of the linear and cubic elastic external forces with the corresponding optimal load resistance is performed.For the nonlinear hardening case,more positive linear coefficient is preferred.For the nonlinear softening case,the cubic coefficient slightly larger than its optimal value is recommended at each given linear coefficient.By tuning the load resistance and linear and cubic coefficients of the external force,broadband bistable energy harvesting with optimized power is realized. | Ting Tan Zhimiao Yan Kejing Ma Fengrui Liu Linchuan Zhao Wenming Zhang | 2020 | Acta Mechanica Sinica2020，36，3： | 1 |
| 17 | 世界最古の土器と稲作の起源显示文摘 | Yan Wenming | 1996 | 季刊考古学1996，，： | 1 |
| 18 | CdWO4 polymorphs: Selective prepara- tion, eleetronie struetures, and photoeatalytic activities显示文摘 | Yan Tingjiang Li Liping Tong Wenming Zheng Jing Wang Yunjian Li Guangshe | 2011 | Jour- nal of Solid State Chemistry2011，184，2： | 1 |
| 19 | Demosaicing of color filter array captured images using gradient edge detection masks and adaptive heterogeneity-projection显示文摘 | CHUNG Kuoliang YANG Weijen YAN Wenming | 2008 | IEEE Trans on Image Processing2008，17，12： | 1 |
| 20 | Guidelines for the diagnosis and treatment of acute pancreatitis in China (2021)显示文摘Acute pancreatitis(AP)is a common acute abdominal condition of the digestive system.In recent years,treatment concepts,methods,and strategies for the diagnosis of AP have advanced,and this has played an important role in promoting the standardization of AP diagnosis and treatment and improving the treatment quality of AP patients.On the basis of previous guidelines and expert consensus,this guideline adopts an evidence-based,problem-based expression;synthesizes important clinical research data at home and abroad in the most recent 5 years;and forms 29 recommendations through multidisciplinary expert discussion,including diagnosis,treatment,and follow-up.It is expected to provide evidence support for the treatment of AP in the clinical setting in China. | Fei Li Shouwang Cai Feng Cao Rufu Chen Deliang Fu Chunlin Ge Chunyi Hao Jihui Hao Heguang Huang Zhixiang Jian Gang Jin Ang Li Haimin Li Shengping Li Weiqin Li Yixiong Li Tingbo Liang Xubao Liu Wenhui Lou Yi Miao Yiping Mou Chenghong Peng Renyi Qin Chenghao Shao Bei Sun Guang Tan Xiaodong Tian Huaizhi Wang Lei Wang Wei Wang Weilin Wang Junmin Wei Heshui Wu Wenming Wu Zheng Wu Changqing Yan Yinmo Yang Xiaoyu Yin Xianjun Yu Chunhui Yuan Taiping Zhang Yupei Zhao on behalf of the Chinese Pancreatic Surgery Association | 2021 | Journal of Pancreatology2021，4，2： | 1 |
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