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| 1 | Efficient and Specific Modifications of the Drosophila Genome by Means of an Easy TALEN Strategy显示文摘技术开发总是是在生物医学的研究驾驶突破的力量之一。自从托马斯·摩根的时间起,一步一步地, Drosophilists 为操作并且机能上地把开发了强大的基因工具为改进这些技术并且开发新技术的果蝇染色体,而是房间仍然大,今天特别作为生物学家开始系统地学习不同模型有机体的功能的 genomics ,包括人,以一种高产量的方式。这里,我们报导,第一次在果蝇,为借助于改进抄写在很高的效率修改果蝇染色体的一个快速、容易、高度特定的方法像使活跃之物的受动器核酸酶(TALEN ) 策略。我们利用了最近发达的单位集会策略装配设计修改黄基因的二特定的 TALEN (在性染色体上) 并且新奇正染色体的基因。TALEN 的 mRNAs 随后被注入果蝇胚胎。从 31.2% 注射 F0 肥沃的苍蝇,我们检测了包含黄基因的可继承的修正。从到可继承的修正的察觉的特定的 TALEN 的建设的全部过程能在一个月以内被完成。在果蝇的这个调停 TALEN 的染色体修正方法的潜在的应用程序被讨论。 | Jiyong Liu Changqing Li Zhongsheng Yu Peng Huang Honggang Wu Chuanxian Wei Nannan Zhu Yan Shen Yixu Chen Bo Zhang Wu-Min Deng Renjie Jiao | 2012 | Journal of Genetics and Genomics2012，39，5： | 43 |
| 2 | Recent Advances in Synthesis of Waterborne Polyurethane and Their Application in Water-based Ink:A Review显示文摘Over the last few years,waterborne polyurethane(WPU) was applied to various fibers,adhesives,primers for metals,caulking materials,emulsion polymerization media,paint additives,defoamers,associate thickeners,pigment pastes,textile dyes and biomaterials,resulting in the increasing enthusiasm of researchers to design and synthesize novel WPU with unique properties.Thus,various processes and raw materials have been developed to prepare WPU.This review gives an overview on the developments of WPU mainly derived from novel polyols,analyzes the potential application in water-based inks and presents the probable future research area about water-based inks.Notably,the poly(ε-caprolactone) and poly(lactic acid) are the versatile materials used in WPU synthesis and supply the potential special performance for preparing WPU.Meanwhile,addition-fragmentation chain transfer(RAFT) polymerization and atom transfer radical polymerization(ATRP) processes provide an opportunity to control the chain sequence of WPU and obtain products with the desired performance. | Xing Zhou Yan Li Changqing Fang Shujuan Li Youliang Cheng Wanqing Lei Xiangjie Meng | 2015 | Journal of Materials Science & Technology2015，31，7： | 47 |
| 3 | Nanomaterials Applied in Asphalt Modification:A Review显示文摘Nanotechnology has been gradually penetrated into the field of asphalt modification.Seemingly magic effects of nanomaterials have now been brought to improve the performance of asphalt.To demonstrate many of the prospective applications,researchers have conducted a series of positive and effective efforts dealing with the preparation of modified asphalt to demonstrate the mechanism of modification and the resultant improvement in performance.In this review,various nanomaterials used in asphalt modification are initially presented,followed by the methods employed to modify the asphalt with these materials and finally the effects of nanomaterials on the performance of base asphalt are presented and the modification mechanisms are discussed.Based on the current research results,the influence of preparation process parameters on the compatibility of every phase in the modified asphalt and the stability of the modified asphalt system are described.Finally,the development trend of the topic field is projected. | Changqing Fang Ruien Yu Shaolong Liu Yan Li | 2013 | Journal of Materials Science & Technology2013，29，7： | 23 |
| 4 | Effect of acupotomy on nitric oxide synthase and beta-endorphin in third lumbar vertebrae transverse process syndrome model rats显示文摘OBJECTIVE: To explore the long-term effects and pain relief mechanism of acupotomy by observing changes in nitric oxide synthase(NOS) and beta-endorphin(β-EP) in the hypothalamus, spinal cord,and peripheral blood of rats with third lumbar vertebrae(L3) transverse process syndrome.METHODS:Twenty-eight SD rats were randomly assigned to normal, model, electroacupuncture(EA),and acupotomy group. The last three groups were put through an operation to emulate L3transverse process syndrome. Fourteen days after the simulation operation, EA and acupotomy treatments were applied to the respective groups. Fifty-six days afterthe simulation operation, biochemistry tests and enzyme-linked immunosorbent assay were used to measure NOS and β-EP in the hypothalamus, spinal cord, and peripheral blood.RESULTS: Rats with the simulation operation showed significantly higher levels of NOS and β-EP in the hypothalamus, spinal cord, and peripheral blood than those in the normal group. The EA and acupotomy groups had significantly lower levels of NOS and β-EP than those in the model group.There was no statistical difference between the EA and acupotomy groups.CONCLUSION: EA and acupotomy treatments significantly lowered NOS and β-EP levels in the hypothalamus, spinal cord, and peripheral blood and alleviated L3transverse process syndrome. | Changqing Guo Naigang Liu Xiaohong Li Hongmei Sun Bo Hu Jing Lu Yan Guo Chuxi Liang Hong Xu Haixia Wu | 2014 | Journal of Traditional Chinese Medicine2014，34，2： | 15 |
| 5 | Cropland redistribution to marginal lands undermines environmental sustainability显示文摘Cropland redistribution to marginal land has been reported worldwide;however,the resulting impacts on environmental sustainability have not been investigated sufficiently.Here we investigated the environmental impacts of cropland redistribution in China.As a result of urbanization-induced loss of high-quality croplands in south China(~8.5 t ha^(-1)),croplands expanded to marginal lands in northeast(~4.5 t ha^(-1)) and northwest China(~2.9 t ha^(-1)) during 1990-2015 to pursue food security.However,the reclamation in these low-yield and ecologically vulnerable zones considerably undermined local environmental sustainability,for example increasing wind erosion(+3.47%),irrigation water consumption(+34.42%),fertilizer use(+20.02%) and decreasing natural habitats(-3.11%).Forecasts show that further reclamation in marginal lands per current policies would exacerbate environmental costs by 2050.The future cropland security risk will be remarkably intensified because of the conflict between food production and environmental sustainability.Our research suggests that globally emerging reclamation of marginal lands should be restricted and crop yield boost should be encouraged for both food security and environmental benefits. | Wenhui Kuang Jiyuan Liu Hanqin Tian Hao Shi Jinwei Dong Changqing Song Xiaoyong Li Guoming Du Yali Hou Dengsheng Lu Wenfeng Chi Tao Pan Shuwen Zhang Rafiq Hamdi Zherui Yin Huimin Yan Changzhen Yan Shixin Wu Rendong Li Jiuchun Yang Yinyin Dou Wenbin Wu Liqiao Liang Bao Xiang Shiqi Yang | 2022 | National Science Review2022，9，1： | 13 |
| 6 | Effect of electro-acupuncture on gene expression in heart of rats with stress-induced pre-hypertension based on gene chip technology显示文摘OBJECTIVE:To explore electro-acupuncture's(EA's)effect on gene expression in heart of rats with stress-induced pre-hypertension and try to reveal its biological mechanism based on gene chip technology.METHODS:Twenty-seven Wistar male rats were randomly divided into 3 groups.The stress-induced hypertensive rat model was prepared by electric foot-shocks combined with generated noise.Molding cycle lasted for 14 days and EA intervene was applied on rats in model + EA group during model preparation.Rat Gene 2.0 Sense Target Array technology was used for the determination of gene expression profiles and the screened key genes were verified by real-time quantitative polymerase chain reaction(RT-PCR) method.RESULTS:Compared with blank control group,390 genes were changed in model group;compared with model control group,330 genes were changed in model + EA group.Significance analysis of gene function showed that the differentially expressed genes are those involved in biological process,molecular function and cellular components.RT-PCR result of the screened key genes is consistent with that of gene chip test.CONCLUTION:EA could significantly lower blood pressure of stress-induced pre-hypertension rats and affect its gene expression profile in heart.Genes that related to the contraction of vascular smooth muscle may be involved in EA's anti-hypertensive mechanism. | Guo Yan Xie Xiaojia Guo Changqing Wang Zhaoyang Liu Qingguo | 2015 | Journal of Traditional Chinese Medicine2015，35，3： | 10 |
| 7 | Salivary mycobiome dysbiosis and its potential impact on bacteriome shifts and host immunity in oral lichen planus显示文摘The biodiversity of the mycobiome,an important component of the oral microbial community,and the roles of fungal–bacterial and fungal–immune system interactions in the pathogenesis of oral lichen planus (OLP) remain largely uncharacterized.In this study,we sequenced the salivary mycobiome and bacteriome associated with OLP.First,we described the dysbiosis of the microbiome in OLP patients,which exhibits lower levels of fungi and higher levels of bacteria.Significantly higher abundances of the fungi Candida and Aspergillus in patients with reticular OLP and of Alternaria and Sclerotiniaceae_unidentified in patients with erosive OLP were observed compared to the healthy controls.Aspergillus was identified as an “OLP-associated” fungus because of its detection at a higher frequency than in the healthy controls.Second,the co-occurrence patterns of the salivary mycobiome–bacteriome demonstrated negative associations between specific fungal and bacterial taxa identified in the healthy controls,which diminished in the reticular OLP group and even became positive in the erosive OLP group.Moreover,the oral cavities of OLP patients were colonized by dysbiotic oral flora with lower ecological network complexity and decreased fungal–Firmicutes and increased fungal–Bacteroidetes sub-networks.Third,several keystone fungal genera (Bovista,Erysiphe,Psathyrella,etc.) demonstrated significant correlations with clinical scores and IL-17 levels.Thus,we established that fungal dysbiosis is associated with the aggravation of OLP.Fungal dysbiosis could alter the salivary bacteriome or may reflect a direct effect of host immunity,which participates in OLP pathogenesis. | Yan Li Kun Wang Bo Zhang Qichao Tu Yufei Yao Bomiao Cui Biao Ren Jinzhi He Xin Shen Joy D.Van Nostrand Jizhong Zhou Wenyuan Shi Liying Xiao Changqing Lu Xuedong Zhou | 2019 | International Journal of Oral Science2019，11，3： | 8 |
| 8 | A fundamental theorem for eco-environmental surface modelling and its applications显示文摘We propose a fundamental theorem for eco-environmental surface modelling(FTEEM) in order to apply it into the fields of ecology and environmental science more easily after the fundamental theorem for Earth’s surface system modeling(FTESM). The Beijing-Tianjin-Hebei(BTH) region is taken as a case area to conduct empirical studies of algorithms for spatial upscaling, spatial downscaling, spatial interpolation, data fusion and model-data assimilation, which are based on high accuracy surface modelling(HASM), corresponding with corollaries of FTEEM. The case studies demonstrate how eco-environmental surface modelling is substantially improved when both extrinsic and intrinsic information are used along with an appropriate method of HASM. Compared with classic algorithms, the HASM-based algorithm for spatial upscaling reduced the root-meansquare error of the BTH elevation surface by 9 m. The HASM-based algorithm for spatial downscaling reduced the relative error of future scenarios of annual mean temperature by 16%. The HASM-based algorithm for spatial interpolation reduced the relative error of change trend of annual mean precipitation by 0.2%. The HASM-based algorithm for data fusion reduced the relative error of change trend of annual mean temperature by 70%. The HASM-based algorithm for model-data assimilation reduced the relative error of carbon stocks by 40%. We propose five theoretical challenges and three application problems of HASM that need to be addressed to improve FTEEM. | Tianxiang YUE Na ZHAO Yu LIU Yifu WANG Bin ZHANG Zhengping DU Zemeng FAN Wenjiao SHI Chuanfa CHEN Mingwei ZHAO Dunjiang SONG Shihai WANG Yinjun SONG Changqing YAN Qiquan LI Xiaofang SUN Lili ZHANG Yongzhong TIAN Wei WANG Ying’an WANG Shengnan MA Hongsheng HUANG Yimin LU Qing WANG Chenliang WANG Yuzhu WANG Ming LU Wei ZHOU Yi LIU Xiaozhe YIN Zong WANG Zhengyi BAO Miaomiao ZHAO Yapeng ZHAO Yimeng JIAO Ufra NASEER Bin FAN Saibo LI Yang YANG John PWILSON | 2020 | Science China Earth Sciences2020，63，8： | 7 |
| 9 | SHRIMP Zircon U-Pb and Molybdenite Re-Os Datings of the Superlarge Donggou Porphyry Molybdenum Deposit in the East Qinling,China,and Its Geological Implications显示文摘摘要:在东方 Qinling 铝带的东方部分定位了, Donggou 存款是在最近的年里发现的 superlarge 斑岩铝存款。作者执行了在 Donggou 铝存款的使矿物化的斑岩和矿石的高度精确的标明日期。Donggou 铝土的 A 类型花岗石斑岩标明日期的虾 U-Pb 锆石给了 112 楬瑡摥 ? 的形成岩石的年龄 ??????? | YE Huishou MAO Jingwen LI Yongfeng GUO Baojian ZHANG Changqing LIU Jun YAN Quanren LIU Guoyin | 2008 | Acta Geologica Sinica(English Edition)2008，82，1： | 6 |
| 10 | Additive manufacturing of CNTs/PLA composites and the correlation between microstructure and functional properties显示文摘Owing to the facile,low cost,rapid,personalization characters,3D printing method has been one of the most attractive additive manufacturing processes in medicine,airplane,packaging and printing areas.In this work,a series of carbon nanotubes/polylactic acid(CNTs/PLA) composites were prepared through the combination of molten co-extrusion and 3D printing processes.The orientation and dispersion of CNTs in PLA matrix were investigated to explore the impact of 3D printing process on the morphology of CNTs/PLA composites via transmission electron microscopy,field emission scanning electron microscopy and Raman spectroscopy.X-ray diffractometer,differential scanning calorimetry,and thermal gravity analysis were employed to study the crystal structure and thermal properties of the composites.In addition,the electrical conductivity of the prepared specimen revealed that the orientation of CNTs in PLA might enhance the conductivity of the composite.It was found that 3D printing process was beneficial to increasing the purity of CNTs,electrical conductivity and mechanical properties of CNTs/PLA composites. | Xing Zhou Jingrui Deng Changqing Fang Wanqing Lei Yonghua Song Zisen Zhang Zhigang Huang Yan Li | 2021 | Journal of Materials Science & Technology2021，，1： | 6 |
| 11 | Correlation of Raw Materials and Waterborne Polyurethane Properties by Sequence Similarity Analysis显示文摘Anionomer-type waterborne polyurethane dispersions(PUDs) were obtained from poly(propylene glycol)(PPG), isophorone diisocyanate(IPDI) and dimethylolpropionic acid(DMPA) through a modi?ed prepolymer isocyanate process. Two series of polyurethanes were prepared(Groups A and B) and a new prediction model based on grey relational analysis is introduced to predict the impact order of raw materials on several properties, such as solids content, viscosity, acid number and electrolytic stability of polyurethanes. It is found that the model can successfully predict the impact of raw materials on the properties through the designed demonstration experiments. Furthermore, the results of the prediction model show that DMPA plays a key role in viscosity, partial acid values and electrolytic stability. | Xing Zhou Changqing Fang Jing Chen Shujuan Li Yan Li Wanqing Lei | 2016 | Journal of Materials Science & Technology2016，32，7： | 5 |
| 12 | Anatomical variation of infra-pyloric artery origination: A prospective multicenter observational study (IPA-Origin)显示文摘Objective: Infra-pyloric artery(IPA) is an important anatomical landmark in treatment of gastric cancer and is the key vessel for pylorus-preserving gastrectomy and subgroup of infra-pyloric lymph nodes. However, its anatomical variation is not thoroughly understood. Our study aimed to clarify the origination of the IPA.Methods: We did this prospective, multicenter, open-label, observational study at gastric surgery departments of34 hospitals in China. Gastric cancer patients aged 18 years or older and scheduled to undergo elective total or distal gastrectomy were assigned. During the surgery, IPA dissecting and exposing the origination point with photographs or video clips were required. The primary outcome was the origination of the IPA. Analysis of variance, χ~2 tests and Fisher's tests were used to analyze the differences between groups. The study is registered at Clinicaltrials.gov(No. NCT03071237).Results: Between May 8 and July 31, 2017, 429 patients were assigned for the study, and 419(97.7%) patients had the IPA dissected and recorded through photograph or video and were included in the primary outcome analysis. The median age was 62 years old, and 73.7% were male. Among the patients, 78.5% received laparoscopic surgery. Single IPA origination was identified in 398(95.0%) patients, including gastroduodenal artery(GDA) in154(36.8%) patients, anterior superior pancreaticoduodenal artery(ASPDA) in 130(31.0%) patients, and right gastroepiploic artery(RGEA) in 114(27.2%) patients. Fifteen(3.6%) patients were identified with multiple IPA and 6(1.4%) patients were identified as IPA absence. The differences in the distribution of surgical approach(P=0.003) and geographic area(P=0.030) were statistically significant. No difference was shown in sex, age,gastrectomy type, tumor location, and clinical T, N and M stage.Conclusions: Our study found that the IPA originates from GDA, ASPDA and RGEA in similar proportions.Laparoscopic surgery may be more helpful in dissection of the IPA than open surgery. | Rulin Miao Jianjun Qu Zhengrong Li Daguang Wang Jiang Yu Weidong Zang Yong Li Fenglin Liu Jian Zhang Wu Song Kai Ye Su Yan Wei Wang Shuangyi Ren Lu Zang Changqing Jing Li Zhang Kuan Wang Weihua Fu Lin Fan Bin Liang Gang Zhao Jun Cai Li Yang Jiaming Zhu Jun You Kun Yang Qingxing Huang Zhaojian Niu Ning Ning Xingfeng Qiu Gang Ji Feng Liang Hua Huang Chao Gao Fei Shan Shuangxi Li Yongning Jia Lianhai Zhang Xiangji Ying Yan Zhang Zhaode Bu Xiangqian Su Gang Zhao Ziyu Li Jiafu Ji | 2018 | Chinese Journal of Cancer Research2018，30，5： | 5 |
| 13 | Enzyme Inhibition Rate Method for Rapid Detection of Organophosphorus and Carbamate Pesticides in Cowpea显示文摘[Objectives] The paper was to explore enzyme inhibition rate method for rapid detection of organophosphorus and carbamate pesticides in cowpea.[Methods]Acetylcholinesterase( ACh E) was added to cowpea extract,to determine the inhibition rate of extract against enzyme. The influences of different sampling methods and sampling parts on detection results were compared. [Results]The positive rate of standard sampling was 18. 18% higher than that of non-standard sampling,and the positive rate of samples collected from cowpea tail was 16. 67% higher than that collected from other parts. [Conclusions] Enzyme inhibition rate method is suitable for rapid detection of organophosphorus and carbamate pesticides in cowpea. | Mai Changqing Chen Sheng Chen Yan | 2017 | Plant Diseases and Pests2017，8，4： | 4 |
| 14 | 胃食管反流病:内镜评估与治疗方面的革新显示文摘内镜技术的创新为胃食管反流病(GERD)的诊治带来了巨大进步。作为世界上最常见的胃肠疾病之一,GERD一直是内镜介入治疗的前沿。目前,GERD的初步诊断是基于症状和质子泵抑制剂(PPI)初期治疗有效,但这对治疗策略的指导价值有限。然而,内镜检查和病理活检可直接观察和判断食管的异常结构和病理生理损伤;同时,内镜治疗的出现给PPI治疗依从性较差或治疗反应不佳以及不适合行胃底折叠抗反流手术的患者提供了新的治疗选择。本综述对内镜检查和活检在经过恰当的抗反流药物治疗后持续存在GERD相关临床表现的患者中的应用情况进行了概述,并介绍了目前内镜治疗GERD的各项新技术及其优缺点。今后的研究需要对GERD患者进行分层研究,并力图阐明该疾病的主要病理生理机制。 | Sheng Chen Feng Du Changqing Zhong Caifang Liu Xiaoying Wang Yan Chen Gang Wang Xiaopei Gao Lu Zhang Lianyong Li Wei Wu | 2021 | Gastroenterology Report2021，9，5： | 4 |
| 15 | Ectopic expression of fungal EcGDH improves nitrogen assimilation and grain yield in rice显示文摘NADP(H)-dependent glutamate dehydrogenases(GDH) in lower organisms have stronger ammonium affinity than those in higher plants. Here we report that transgenic rice overexpressing the EcGDH from Eurotium cheralieri exhibited significantly enhanced aminating activities. Hydroponic and field tests showed that nitrogen assimilation efficiency and grain yields were markedly increased in these transgenic plants, especially at the low nitrogen conditions.These results suggest that EcGDH may have potential to be used to improve nitrogen assimilation and grain yield in rice. | Dongying Tang Yuchong Peng Jianzhong Lin Changqing Du Yuanzhu Yang Dan Wang Cong Liu Lu Yan Xiaoying Zhao Xia Li Liangbi Chen Xuanming Liu | 2018 | Journal of Integrative Plant Biology2018，60，2： | 4 |
| 16 | Probucol attenuates ethanol‐induced liver fibrosis in rats by inhibiting oxidative stress, extracellular matrix protein accumulation and cytokine production显示文摘 | Xuesong Su Yanqiu Wang Guangyu Zhou Xu Yang Rui Yu Yan Lin Changqing Zheng | 2014 | Clin Exp Pharmacol Physiol2014，，1： | 3 |
| 17 | MicroRNA-21 suppresses PTEN and hSulf-1 expression and promotes hepatocellular carcinoma progression through AKT/ERK pathways显示文摘 | Longlong Bao Yan Yan Can Xu Weidan Ji Shuwen Shen Gaoya Xu Yong Zeng Bin Sun Haihua Qian Lei Chen Mengchao Wu Changqing Su Jie Chen | 2013 | Cancer Letters2013，，2： | 3 |
| 18 | Influence of salinity and water content on soil microorganisms显示文摘Salinization is one of the most serious land degradation problems facing world.Salinity results in poor plant growth and low soil microbial activity due to osmotic stress and toxic ions.Soil microorganisms play a pivotal role in soils through mineralization of organic matter into plant available nutrients.Therefore it is important to maintain high microbial activity in soils.Salinity tolerant soil microbes counteract osmotic stress by synthesizing osmolytes which allows them to maintain their cell turgor and metabolism.Osmotic potential is a function of the salt concentration in the soil solution and therefore affected by both salinity(measured as electrical conductivity at a certain water content)and soil water content.Soil salinity and water content vary in time and space.Understanding the effect of changes in salinity and water content on soil microorganisms is important for crop production,sustainable land use and rehabilitation of saline soils.In this review,the effects of soil salinity and water content on microbes are discussed to guide future research into management of saline soils. | Nan Yan Petra Marschner Wenhong Cao Changqing Zuo Wei Qin | 2015 | International Soil and Water Conservation Research2015，3，4： | 3 |
| 19 | Criteria for the growth of large-area adlayer-free monolayer graphene films by chemical vapor deposition显示文摘Homogeneity is important to material applications for good performance of individual devices,for making AB-stacked bilayer graphene in a layer-by-layer stacking order,and from the point of view of industrial production.Among many properties to be controlled,for the case of graphene,the thickness(or layer number)uniformity is the prerequisite.Chemical vapor deposition(CVD)of C precursors on Cu substrates is the most popular method to produce large-area graphene films.To date,precise control on the number of graphene layers as well as the uniformity over a large area is still very challenging.In this work,with a further understanding of the factors affecting adlayer growth,the synthesis of large-area adlayer-free monolayer graphene(MLG)films was achieved up to tens of squared centimeters in area by just using untreated Cu foil and a normal CVD process.We found that keeping equal C precursor concentration on the two sides of the Cu substrate is a criterion in addition to other factors such as the ratio of H:C and the substrate surface morphology for the growth of adlayer-free MLG.This finding is not only of great significance for the industrial production of large-area adlayer-free MLG films but also instructive for the synthesis of homogeneous few-layer graphene. | Changqing Shen Xingzhou Yan Fangzhu Qing Xiaobin Niu Richard Stehle Samuel S.Mao Wanli Zhang Xuesong Li | 2019 | Journal of Materiomics2019，5，3： | 2 |
| 20 | A simple daily dynamic feeding regimen for reducing phosphorus consumption and excretion in laying hens显示文摘Phosphorus metabolism in laying hens is a highly dynamic process over the course of the 24 h egg-laying cycle.Adjusting the phosphorus feeding regimen according to the daily egg-laying cycle may help to improve phosphorus utilization efficiency.Hy-Line Brown layers(n=120;70 wk old)were offered 4different phosphorus daily regimens:(1)RR,fed regular phosphorus at both 09:00 and 17:00;(2)RL,fed regular phosphorus at 09:00 and low phosphorus at 17:00;(3)LR,fed low phosphorus at 09:00 and regular phosphorus at 17:00;(4)LL,fed low phosphorus at both 09:00 and 17:00.The regular and low phosphorus diets contained 0.32% and 0.14% non-phytate phosphorus,respectively.The feeding trial lasted for 12 wk.As a result,layers on the RL regimen had decreased laying rate(P<0.05;5 to 8,9 to 12,and 1 to 12 wk)when compared to all other regimens.Layers on the LL regimen had decreased eggshell thickness and specific gravity(P<0.05;wk 8)when compared to all other regimens,and had decreased egg shell strength(P<0.05;wk 8)when compared to RL and LR regimens.When compared to the RR regimen(a common practice in the industry),layers on the LR regimen had:(1)identical laying performance and egg quality(P>0.05);(2)decreased phosphorus excretion(P<0.05)during the period of09:00 to 17:00;(3)increased jejunal calbindin D28k protein expression(P<0.05)2 h after feeding in the morning;(4)decreased serum fibroblast growth factor 23 and calcitriol levels(P<0.05),decreased jejunal type Ⅲ sodium-phosphate cotransporter 2 gene and protein expression(P<0.05),and decreased renal type Ⅲ sodium-phosphate cotransporter 1 protein expression(P<0.05),2 h after feeding in the afternoon.In summary,when dietary phosphorus was supplemented in accordance with daily serum phosphorus rhythms(i.e.,the LR regimen),laying performance and egg quality were well supported whilst significantly decreasing phosphorus consumption and excretion.Thus,serum phosphorus rhythms will need to be carefully maintained when developing dietary phosphorus-reduction strategies in laying hens. | Xujie Liao Jiakun Yan Jionghao Chen Zhenyu Huang Tianshuai Xiao Changqing Li Chong Pan Xin Yang Yanli Liu Thomas D.Crenshaw Xiaojun Yang Zhouzheng Ren | 2022 | Animal Nutrition2022，，4： | 2 |
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