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| 1 | Substantial transition to clean household energy mix in rural China显示文摘The household energy mix has significant impacts on human health and climate,as it contributes greatly to many health-and climate-relevant air pollutants.Compared to the well-established urban energy statistical system,the rural household energy statistical system is incomplete and is often associated with high biases.Via a nationwide investigation,this study revealed high contributions to energy supply from coal and biomass fuels in the rural household energy sector,while electricity comprised∼20%.Stacking(the use of multiple sources of energy)is significant,and the average number of energy types was 2.8 per household.Compared to 2012,the consumption of biomass and coals in 2017 decreased by 45%and 12%,respectively,while the gas consumption amount increased by 204%.Increased gas and decreased coal consumptions were mainly in cooking,while decreased biomass was in both cooking(41%)and heating(59%).The time-sharing fraction of electricity and gases(E&G)for daily cooking grew,reaching 69%in 2017,but for space heating,traditional solid fuels were still dominant,with the national average shared fraction of E&G being only 20%.The non-uniform spatial distribution and the non-linear increase in the fraction of E&G indicated challenges to achieving universal access to modern cooking energy by 2030,particularly in less-developed rural and mountainous areas.In some non-typical heating zones,the increased share of E&G for heating was significant and largely driven by income growth,but in typical heating zones,the time-sharing fraction was<5%and was not significantly increased,except in areas with policy intervention.The intervention policy not only led to dramatic increases in the clean energy fraction for heating but also accelerated the clean cooking transition.Higher income,higher education,younger age,less energy/stove stacking and smaller family size positively impacted the clean energy transition. | Guofeng Shen Rui Xiong Yanlin Tian Zhihan Luo Bahabaike Jiangtulu Wenjun Meng Wei Du Jing Meng Yuanchen Chen Bing Xue Bin Wang Yonghong Duan Jia Duo Fenggui Fan Lei Huang Tianzhen Ju Fenggui Liu Shunxin Li Xianli Liu Yungui Li Mu Wang Ying Nan Bo Pan Yanfang Pan Lizhi Wang Eddy Zeng Chao Zhan Yilin Chen Huizhong Shen Hefa Cheng Shu Tao | 2022 | National Science Review2022，9，7： | 4 |
| 2 | Homomorphic MAC-Based Scheme against Pollution Attacks in Network Coding显示文摘Network coding can improve network throughput in large, but it is vulnerable to the data pollution attacks. In this paper, we propose an efficient homomorphic message authentication code (MAC) scheme with discrete logarithm to detect and locate the malicious nodes. We also prove the security property of the scheme theoretically. Its effectiveness is demonstrated, and overhead is analyzed through extensive experiments. | ZHANG Yujie CAI Ying LI Zhuo FAN Yanfang | 2013 | Wuhan University Journal of Natural Sciences2013，18，5： | 3 |
| 3 | Expression of P-gp and miRNA-21 in Colorectal Cancer Tissues and the Clinical Significance显示文摘[Objectives]To study the expression of P-gp and miRNA-21 in colorectal cancer tissues and the relationship between the expression of P-gp and clinicopathological features,and to analyze the correlation between the expression of miRNA-21 and P-gp.[Methods]Immunohistochemical SP method was used to detect the expression level of P-gp in the tissues of 40 cases of colorectal cancer and the normal mucosal tissues 5 cm away from the corresponding cancer tissues,and the expression level of miRNA-21 in the paraffin tissues of the 40 cases was detected by RT-qPCR.[Results]The positive expression of P-gp was localized in the cell membrane,and the expression in colorectal cancer tissues was significantly higher than that in the corresponding adjacent tissues(P<0.05);the positive expression of P-gp was closely related to the degree of tumor differentiation and the depth of invasion(P<0.05),and the comparison results of P-gp expression in the differentiation groups of different cancer tissues showed that the expression level of P-gp in the moderately and poorly differentiated groups was significantly higher than that in the highly differentiated group(P<0.05),and the expression level of the moderately and poorly differentiated groups was similar(P>0.05).The expression of miRNA-21 in all cancer tissues was higher than that in the corresponding adjacent tissues(P<0.05),and the expression levels of miRNA-21 in differentiation groups of different cancer tissues were 1.286,1.445 and 1.992 times of those in the corresponding adjacent tissues,respectively;the comparison results of miRNA-21 between different differentiation groups showed that the expression level of miRNA-21 in the poorly differentiated group was significantly higher than that in the highly differentiated group and the moderately differentiated group(P<0.05),while the expression level of miRNA-21 in the highly differentiated group and the moderately differentiated group was similar(P>0.05).Spearman correlation analysis showed that there was a positive correlation on the expression between the two in colorectal cancer tissues(r=0.423,P<0.05).[Conclusions]The expression levels of miRNA-21 and P-gp are closely related to the differentiation degree of clinical colorectal cancer,the lower the differentiation degree,the higher the expression level of the two. | Xiaoli YAN Peng YAN Yanfang FAN Jing XUE Mingjuan WANG Jining ZHENG | 2020 | Medicinal Plant2020，11，6： | 1 |
| 4 | Rnf20 deficiency in adipocyte impairs adipose tissue development and thermogenesis显示文摘RNF20,an E3 ligase critical for monoubiquitination of histone H2B at lysine 120(H2Bub),has been implicated in the regulation of various cellar processes;however,its physiological roles in adipocytes remain poorly characterized.Here,we report that the adipocyte-specific knockout of Rnf20(ASKO)in mice led to progressive fat toss,organomegaly and hyperinsulinemia.Despite signs of hyperinsulinemia,normal insulin sensitivity and improved glucose tolerance were observed in the young and aged CD-fed ASKO mice.In addition,high-fat diet-fed ASKO mice developed severe liver steatosis.Moreover,we observed that the ASKO mice were extremely sensitive to a cold environment due to decreased expression levels of brown adipose tissue(BAT)selective genes,including uncoupling protein 1(l/cpf),and impaired mitochondrial functions.Significantly decreased levels of peroxisome proliterator-activated receptor gamma(Ppary)were observed in the gonadal white adipose tissues(gWAT)from the ASKO mice,suggesting that Rnf20 regulates adipogenesis,at least in part,through Ppary.Rosiglitazone-treated ASKO mice exhibited increased fat mass compared to that of the non-treated ASKO mice.Collectively,our results illustrate the critical role of RNF20 in control of white and brown adipose tissue development and physiological function. | Xiaojuan Liang Cong Tao Jianfei Pan Lilan Zhang Lulu Liu Ying Zhao Yiping Fan Chunwei Cao Jiali Liu Jin Zhang Sin Man Lam Guanghou Shui Wanzhu Jin Wei Li Jianguo Zhao Kui Li Yanfang Wang | 2021 | Protein & Cell2021，12，6： | 1 |
| 5 | Extracellular vesicles as novel biomarkers and pharmaceutic targets of diseases显示文摘 | Yanfang CHEN Yaoliang TANG Guo-Chang FAN Dayue Darrel DUAN | 2018 | Acta Pharmacologica Sinica2018，39，4： | 1 |
| 6 | Optimisation of Ultrasound‐assisted Extraction of Puerarin and Total Isoflavones from Puerariae Lobatae Radix ( Pueraria lobata (Wild.) Ohwi) with Response Surface Methodology显示文摘 | Yanfang Wu Xinsheng Wang Enguo Fan | 2012 | Phytochem Anal2012，，5： | 1 |
| 7 | A Survey on Routing Algorithms for Opportunistic Mobile Social Networks显示文摘Opportunistic Mobile Social Networks(OMSNs)are kind of Delay Tolerant Networks(DTNs)that leverage characteristics of Mobile Ad Hoc Networks(MANETs)and Social Networks,particularly the social features,to boost performance of routing algorithms.Users in OMSNs communicate to share and disseminate data to meet needs for variety of applications.Such networks have attracted tremendous attention lately due to the data transmission requirement from emerging applications such as IoT and smart city initiatives.Devices carried by human is the carrier of message transmission,so the social features of human can be used to improve the ability of data transmission.In this paper,we conduct a comparative survey on routing algorithms in OMSNs.We first analyze routing algorithms based on three social features.Since node selfishness is not really considered previously in aforementioned routing algorithms,but has significant impact on network performance,we treat node selfishness as another social feature,classify and elaborate routing algorithms based on incentive mechanism.To assess the impact of social features on routing algorithms,we conducted simulation for six routing algorithms and analyzed the simulation result.Finally,we conclude the paper with challenges on design of routing in OMSNs and point out some future research directions. | Ying Cai Haochen Zhang Yanfang Fan Hongke Xia | 2021 | China Communications2021，18，2： | 1 |
| 8 | Expression and Significance of MicroRNA-21 and P-gp in Colon Cancer Cells显示文摘[Objectives]To investigate the expression and significance of miR-21 and P-gp in colon cancer cell line HCT-15.[Methods]RT-qPCR was used to detect the miR-21 and ABCB1 mRNA expression level of P-gp coding genes in normal colon mucosal cell NCM460 and colon cancer cell HCT-15,and Western Blot was used to detect the expression of P-gp in these two cell lines.[Results]The results of RT-qPCR showed that the expression level of miR-21 and ABCB1 mRNA in colon cancer cell HCT-15 was significantly higher than that in normal colon cell NCM460,and the difference was statistically significant(P<0.05).Western Blot results showed that the expression level of P-gp in HCT-15 was significantly higher than that in NCM460,and the difference was statistically significant(P<0.05).[Conclusions]The expression level of miR-21 and ABCB1 mRNA/P-gp in colon cancer cell HCT-15 was higher than that in normal colonic mucosal cell NCM460,and the relationship between miR-21 and multidrug resistance in colon cancer might be related to P-gp. | Xiaoli YAN Yanfang FAN Peng YAN Jing XUE Zhenhong LIU Jining ZHENG | 2020 | Medicinal Plant2020，11，2： | 1 |
| 9 | Research on the protection coordination of permanent magnet synchronous generator based wind farms with low voltage ride through capability显示文摘To coordinate the protection of PMSG(permanent magnet synchronous generator),collector circuits and outgoing lines,a comprehensive and improved protection method of PMSG based wind farms with LVRT(low voltage ride through)capability is proposed.The proposed method includes adding a short time delay to the collector network current protection zone I and a directional protective relaying to the collector network protection,installing grounding transformers and zero sequence current protection,and generator low-voltage protection action improvement.A LVRT scheme consisting of variable resistance dumping circuit,grid side dynamic reactive power control and reactive power compensation control is proposed.The fault characteristics of PMSG based wind farms are analyzed,and a PMSG based wind farm in Dabancheng,Xinjiang,is used as an example to analyze typical wind farm protection configuration,the setting values considering LVRT requirements,and the coordination problems.Finally,an improved wind farm protection coordination methodology is proposed and its validity is verified by simulation. | Renfeng Tao Fengting Li Weiwei Chen Yanfang Fan Chenguang Liang Yang Li | 2017 | Protection and Control of Modern Power Systems2017，2，1： | 1 |
| 10 | Structural elucidation of a neutral fucogalactan from the mycelium of Coprinus comatus显示文摘 | Fan Junmin Zhang Jingsong Tang Qingjiu Liu Yanfang Zhang Anqiang Pan Yingjie | 2006 | Carbohydrate Research2006，341，9： | 1 |
| 11 | Effect of ATM/CHK2/CDC25A Signal Pathway on the Resistance of Colorectal Cancer Cells to L-OHP显示文摘[Objectives]To compare the expression of key factors ATM,CHK2,CDC25A and CDK2 in ATM/CHK2/CDC25A signal pathway in colorectal cancer cell line HCT116 and L-OHP-resistant cell line HCT116/L-OHP,and to explore the relationship between ATM/CHK2/CDC25A signal pathway and resistance of colorectal cancer cells to L-OHP.[Methods]RT-qPCR was used to detect the expression of ATM,CHK2,CDC25A and CDK2 mRNA in colorectal cancer cell line HCT116 and L-OHP-resistant cell line HCT116/L-OHP,and Western blot was used to detect the expression of corresponding proteins in these two cell lines.[Results]The expression of ATM,CHK2,CDC25A,CDK2 mRNA and protein in HCT116/L-OHP was significantly higher than that in HCT116.[Conclusions]ATM/CHK2/CDC25A signal pathway was associated with resistance of colorectal cancer cell line HCT116 to L-OHP.Blocking this signal pathway may partially reverse the chemotherapy resistance of colorectal cancer patients with L-OHP resistance. | Xiaoli YAN Peng YAN Jing XUE Yanfang FAN Jining ZHENG | 2021 | Medicinal Plant2021，12，3： | 1 |
| 12 | Structures and Receptor Binding of Hemagglutinins from Human-Infecting H7N9 Influenza Viruses显示文摘 | Yi Shi Wei Zhang Fei Wang Jianxun Qi Ying Wu Hao Song Feng Gao Yuhai Bi Yanfang Zhang Zheng Fan Chengfeng Qin Honglei Sun Jinhua Liu Joel Haywood Wenjun Liu Weimin Gong Dayan Wang Yuelong Shu Yu Wang Jinghua Yan George F. Gao | 2013 | Science2013，，6155： | 1 |
| 13 | Effects of Paeonol on CRP and NF-κB p65 in Rats with Atherosclerosis显示文摘[Objectives] The protective mechanism of paeonol on atherosclerotic vessels was investigated by detecting CRP content in the serum and expression of NF-κB p65 in the aorta of atherosclerotic rats. [Methods] An animal model of AS rats was prepared by high-fat diet combined with intraperitoneal injection of VD3. The rats were randomly divided into the normal group,model group,high-dose paeonol group( 120 mg/kg),medium-dose paeonol group( 60 mg/kg),low-dose paeonol group( 30 mg/kg),and simvastatin group( 10 mg/kg) as the positive control group,and drug intervention was continued for 16 weeks. CRP content in the serum was detected by ELISA method,and the expression level of NF-κB p65 was detected by RT-PCR and Western-blot method. [Results]Compared with the normal group,CRP content in the serum and the expression of NF-κB p65 mRNA and protein in the aorta of rats in the model group significantly increased. Compared with the model group,CRP content in the serum dropped obviously,and the expression of NF-κB p65 mRNA and protein in the aorta of rats significantly reduced in the administration groups. Moreover,the improvement of each indicator in the high-dose paeonol group was obviously better than that of the medium-dose and low-dose groups. [Conclusions] Paeonol may protect atherosclerotic vessels by reducing serum CRP content,inhibiting the expression of NF-κB p65 and reducing inflammation. | Yanfang FAN Yanan ZHAO Kaihang SHI Zhuoya JIA Xiaohui ZHOU | 2019 | Medicinal Plant2019，10，3： | 0 |
| 14 | Controlled release of dexamethasone from porous PLGA scaffolds under cyclic loading显示文摘Poly(L-lactide)-b-poly(ethylene glycol)(PLLA-PEG) microspheres containing dexamethasone(Dex) have been fabricated using a spray-drying technique.Porous poly(lactic-co-glycolic acid)(PLGA) scaffolds were prepared using a method combining thermally induced phase separation and porogen leaching.A post-seeding technique was used to immobilize Dex-containing PLLA-PEG microspheres on porous PLGA scaffolds,and drug-containing microspheres-scaffolds(MS-S) were obtained.Simple Dex-containing scaffolds(D-S) were also made as the control by directly dissolving Dex in the PLGA solution during scaffold fabrication.The morphologies of microspheres and scaffolds were studied by scanning electron microscopy.Drug release profiles of both MS-S and D-S were determined under cyclic loading and shaking water bath,respectively.The cumulative release of Dex was measured using an ultraviolet visible spectrophotometer.The results show that the incorporation of Dex and microspheres had little effect on the overall morphology of the porous PLGA scaffolds.Cyclic loading significantly accelerated the release of Dex from the drug-containing scaffolds.Compared with D-S,MS-S reduced the drug release rate.The controlled drug delivery of tissue engineering scaffolds under cyclic loading is a key factor to mimic the in vivo mechanical environments and achieve optical clinical efficacy. | Tang GongWen Yang YanFang Sun AiPing Song TianTian Zhao YunHui Yuan XuBo Yuan XiaoYan Fan YuBo Wang Min | 2010 | Science China Chemistry2010，53，3： | 0 |
| 15 | Tuning the optoelectronic properties of vinylene linked perylenediimide dimer by ring annulation at the inside or outside bay positions for fullerene-free organic solar cells显示文摘Among various perylenediimide(PDI)-based small molecular non-fullerene acceptors(NFAs),PDI dimer can effectively avoid the excessive aggregation of single PDI and improve the photovoltaic performance.However,the twist of perylene core in PDI dimer will destroy the effective conjugation.Thus,ring annulation of PDI dimer is a feasible method to balance the film quality and electron transport,but the systematic study has attracted few attentions.Herein,we choose a simple vinylene linked PDI dimer,V-PDI2,and then conduct further studies on the structure-property-performance relationship of four kinds of derived fused-PDI dimers,namely V-TDI2,V-FDI2,V-PDIS2 and V-PDISe2 respectively.The former two are incorporated thianaphthene and benzofuran at the inside bay positions,and the latter two are fused thiophene and selenophene at the outside bay positions,respectively.Theoretical calculations reveal the inside-and outside-fused structures largely affect the skeleton configuration,the former two tend to be planar structure and the latter two maintain the distorted backbone.The photovoltaic characterizations show that the inside-fused PDI dimers offer high open circuit voltage(VOC),while the outside-fused PDI dimers afford large short-circuit current density(JSC).This variation tendency results from the reasonably tunable energy levels,light absorption,molecular crystallinity and film morphology.As a result,PBDB-T:V-PDISe2 device exhibits the highest power conversion efficiency(PCE)of 6.51%,and PBDB-T:VFDI2 device realizes the highest VOC of 1.00 V.This contribution indicates that annulation of PDI dimers in outside or inside bay regions is a feasible method to modulate the properties of PDI-based non-fullerene acceptors. | Jing Yang Fan Chen Peiqing Cong Hongjun Xiao Yanfang Geng Zhihui Liao Lie Chen Bao Zhang Erjun Zhou | 2020 | Journal of Energy Chemistry2020，29，1： | 0 |
| 16 | Effect of compound Sophorae decoction in the treatment of ulcerative colitis by tissue extract metabolomics approach显示文摘OBJECTIVE: To investigate how compound Sophorae decoction(CSD) works on rats' models of ulcerative colitis(UC) induced by 2,4,6-trinitrobenzenesulfonic acid solution(TNBS) by metabolomics studies of colon, liver, and kidney tissue extracts.METHODS: Rats with UC induced by TNBS enema were used as models in this study. Metabolic profiles of the three tissues were analyzed and pathway analysis of biomarkers was performed after CSD administration and further integration of metabolic networks.RESULTS: Thirteen biomarkers were screened from colon, liver, and kidney tissue extracts, and the levels of these substances were up-or down-regulated in the model group, but their levels were reversed after CSD administration. These biomarkers were mainly related to Phenylalanine, tyrosine and tryptophan biosynthesis, Phenylalanine metabolism, Glutathione metabolism, Arachidonic acid metabolism, Nicotinate and nicotinamide metabolism,Alanine, aspartate and glutamate metabolism.CONCLUSION: CSD could significantly ameliorate the symptoms of UC by regulating multiple metabolic pathways. | HONG Zongchao CAI Quan DUAN Xueyun YANG Yanfang WU Hezhen JIANG Nan FAN Heng | 2021 | Journal of Traditional Chinese Medicine2021，41，3： | 0 |
| 17 | Protective Effect of Paeonol on Diabetic Cardiomyopathy in Rats显示文摘[Objectives] The protective effect of paeonol on DCM was further confirmed by observing the changes of myocardial structure and function in rats. [Methods] Rats were fed high-sugar and high-fat diet and injected intraperitoneally with streptozotocin(STZ) for two consecutive days to prepare animal model of type II diabetes. The model rats were randomly divided into model group, low-dose paeonol group(30 mg/kg), middle-dose paeonol group(60 mg/kg), high-dose paeonol group(120 mg/kg) and metformin group(157 mg/kg). The detected indicators included heart weight index(HWI), blood glucose(FBG), body mass, triglyceride(TG), and total cholesterol(TC). Myocardial pathological changes were observed by HE staining. The extent of myocardial fibrosis was observed by Masson staining. [Results] In the model group, the body weight of the rats declined significantly, and the phenomena of overdrinking, overeating and polyuria occurred. Compared with the model group, the body weight of rats in the paeonol treatment groups increased to different extents. Compared with the normal group, the HWI, FBG, LDH, AST, and TG increased in the model group, and decreased in different degrees in the paeonol treatment groups(P<0.05), suggesting that paeonol has certain protective effect on diabetic cardiomyopathy in rats. After HE staining and fixation, the myocardial tissue of rats was observed by optical microscope. The morphology of myocardial cells and myocardial fibers in the normal group were normal;Compared with the normal group, the model group has hypertrophy of myocardial cells, disordered arrangement of myocardial fibers, fiber breakage and dissolution, uneven staining between myocardial cells, and nucleus breakage or even disappearance;Compared with the model group, the pathological changes of myocardium in the paeonol treatment groups and DMBG group have been improved to different degrees. The arrangement of myocardial fibers is regular and the coloration is uniform. The results of Masson staining showed that in the normal group, and there was no significant increase of myocardial collagen fibers in the myocardial interstitium. Compared with the normal group, myocardial cells and interstitial collagen fibers(blue) in the model group were significantly increased. Compared with model group, myocardial collagen fiber hyperplasia in the paeonol treatment groups and DMBG group showed different degrees of improvement, among which the Pae-H group had the least blue bands and the most obvious improvement of collagen fiber. [Conclusions] Paeonol has different degrees of improvement effects on myocardial fibrosis in rats with diabetic cardiomyopathy, suggesting that paeonol has a protective effect on diabetic cardiomyopathy in rats. | Ya'nan ZHAO Dandan WU Yanfang FAN Kaixing SHI Xiaohui ZHOU Kai CAO | 2019 | Medicinal Plant2019，10，2： | 0 |
| 18 | Preparation of Acute Myocardial Infarction Model for SD Rats and Ventricular Remodeling显示文摘[Objectives] To prepare an acute myocardial infarction model for SD rats and conduct ventricular remodeling. [Methods] 45 male Sprague-Dawley( SD) rats were randomly selected in 60 male rats as the model group and the rest rats were sham operation group. For the model group,the left anterior descending coronary artery was ligated,while the sham operation group was not ligated. 4 weeks after the operation,hemodynamics was used to measure the cardiac function of both groups. HE staining and Masson staining were used to observe the changes in myocardial histopathology and myocardial fibrosis of both groups. [Results] Compared with the sham operation group,the model group rats showed significant decrease in the left ventricular systolic pressure( LVSP) and left ventricular pressure maximum rising and dropping rate( ± d_p/d_(tmax)) and significant increase in the left ventricular end-diastolic pressure( LVEDP). HE staining showed that a large number of myocardial cell necrosis occured in the model group,while the morphology of myocardial cells of rats in the sham operation group was normal. Masson staining showed that myocardial collagen fibers in the model group were significantly increased compared with those in the sham operation group. Collagen fibers were scattered in the sham operation group. [Conclusions] It can be concluded that cardiac function of rats changed and ventricular remodeling occurred in 4 weeks after myocardial infarction. | Kaihang SHI Zhuoya JIA Yanfang FAN Yanan Zhao Xiaohui ZHOU Kai CAO | 2017 | Medicinal Plant2017，8，6： | 0 |
| 19 | Development and Application of a High-Volume Recycled Powder Solidifying Material for Waterworks Sludge显示文摘Recycled powder(RP)is produced as a by-product during the process of recycling construction and demolition(C&D)wastes,presenting a low additional value.Using RP-based solidifying material can not only improve its utilization efficiency,but also reduce the cost of commercial solidifying materials.To date,this is the best solidifying material utilized to dispose the original waterworks sludge(OWS)with high moisture contents(60%),and the product could be used to fabricate non-fired bricks.This has become a new environment-friendly technology of“using waste to treat waste”.In this paper,the influence of different particle sizes and dosages of RP on the prepared solidifying material was studied.Besides,unconfined compression strength(UCS),volume stability,chemical composition,and heat of hydration,pore structure of the solidifying material were characterized.Then,non-fired bricks were prepared by using the solidifying material,recycled aggregate,and original waterworks sludge.The UCS and softing coefficient(SC)of the non-fired bricks were evaluated.As a result,the 28-day UCS of the solidifying material with optimal(M30)was 35.40 MPa,which could reach 84.37%of Portland cement(PC).The addition of RP increased the volume stability of the solidifying material.The addition of a large amount of RP reduced the heat flux and cumulative heat release of the solidifying material,while its porosity increased.The UCS of non-fired brick(NF20)in 28 days was 15.19 MPa and the SC after 28 days was 78.35%.In conclusion,the preparation of solidifying material using RP could be a promising approach and has a great potential in disposal of original waterworks sludge. | Xiang Deng Sudong Hua Fan Xia Yanfang Zhang Dapeng Guo Xinxing Zhu Defei Zhu | 2022 | Journal of Renewable Materials2022，10，4： | 0 |
| 20 | Expression of Avian Reovirus (ARV) σA Protein in HEK293T Cells显示文摘[Objective]The paper was to construct eukaryotic expression vector of Avian reovirus(ARV)σA gene and expressσA protein accurately in HEK293T cells.[Method]The specific primers of ARVσA gene were designed according to the gene sequence of ARV S2 gene in GenBank(accession number KF741763.1).With pMD18-T-σA recombinant vector as the template,the specific sequence ofσA gene was amplified by PCR and cloned into pMD18-T vector to construct recombinant plasmid.The cloning vector pMD18-T-σA and eukaryotic expression plasmid pEF1α-HA were double digested by restriction enzymes Kpn I and Not I.The purifiedσA gene was connected with pEF1α-HA to construct eukaryotic expression plasmid pEF1α-HA-σA.After colony PCR,double enzyme digestion and sequencing,the recombinant plasmid pEF1α-HA-σA was tansfected into HEK293T cells.The proteins were collected at 24 h after tansfection and verified by Western-blot.[Result]The ARVσA gene was successfully cloned in the test.The eukaryotic expression plasmid pEF1α-HA-σA was constructed,which could be expressed in HEK293T cells.[Conclusion]The protein could be accurately expressed in HEK293T cells. | Ren Hongyu Xie Zhixun Xie Liji Wang Sheng Huang Jiaoling Fan Qing Luo Sisi Zhang Yanfang Zeng Tingting Zhang Mingxiu Xie Zhiqin Deng Xianwen | 2019 | Animal Husbandry and Feed Science2019，11，5： | 0 |
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