|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Extensive translation of circular RNAs driven by N6-methyladenosine显示文摘广泛的 pre-mRNA 拼接背在人的 transcriptome 产生众多的圆形的 RNA (circRNAs ) 。然而,这些 circRNAs 的生物功能仍然保持大部分不清楚。这里,我们报导那 N 6-methyladenosine (m 6 一) , RNA 的最丰富的基础修正,在人的房间从 circRNAs 支持蛋白质翻译的有效开始。我们发现那一致 m 6 A 主题在 circRNAs 和单个 m 6 一个地点是足够的驾驶翻译开始。这 m 6A 驱动的翻译要求开始因素 eIF4G2 和 m 6 读者 YTHDF3,并且被 methyltransferase METTL3/14 提高,在热吃惊之上由 demethylase FTO,和 upregulated 禁止了。通过介绍的 polysome 的进一步的分析,计算预言和集体 spectrometry 揭示那 m 6 circRNAs 的 A 驱动的翻译是普遍的,与有翻译潜力的几百内长的 circRNAs。我们的学习扩展人的 transcriptome 的编码风景,并且在对环境应力的细胞的回答建议导出 circRNA 的蛋白质的一个角色。 | Yun Yang Xiaojuan Fan Miaowei Mao Xiaowei Song Ping Wu6,7 Yang Zhang Yongfeng Jin Yi Yang Ling-Ling Chen Yang Wang Catherine CL Wong Xinshu Xiao Zefeng Wang | 2017 | Cell Research2017，27，5： | 246 |
| 2 | A Pid3 allele from rice cultivar Gumei2 confers resistance to Magnaporthe oryzae显示文摘瑞斯强风,由 Magnaporthe oryzae 引起了,是大多数破坏疾病之一。用基于地图的策略并且在 silico 途径,我们孤立新米饭(Oryza sativa L.) Pid3 的强风抵抗等位基因,指明的 Pi25,从稳定的强风抵抗栽培变种 Gumei2。在表示上分析和互补测试证明 Pi25 授与强风抵抗到 M。oryzae 孤立 js001-20。顺序分析证明 Pi25 是有与在核苷酸位置的 Pid3 相比的单个核苷酸替换的 2772 核苷酸的 intronless 基因 459 并且述词地编码了典型卷的 coilnucleotide 绑定 siteleucine 有到 Pid3 的 100% 身份的 924 氨基酸剩余的富有的重复(CCNBSLRR ) 蛋白质通常认为的蛋白质。在 Nipponbare 的易受影响的等位基因 pi25 在核苷酸位置包含了一个胡说八道变化 2209 与 736 氨基酸剩余导致截断的蛋白质。另外,导致 10 氨基酸替换的 14 核苷酸替换在 Pi25 和在上游的 pi25 之间被识别在易受影响的等位基因的早熟的站 codon。尽管 Pi25/Pid3-mediated 抵抗的机制需要进一步被调查,等位基因的隔离将在经由转基因的途径引起节目的米饭强风抵抗便于 Pi25/Pid3 的利用,标记帮助选择。 | Jie Chen Yongfeng Shi Wenzheng Liu Rongyao Chai Yaping Fu Jieyun Zhuang Jianli Wu | 2011 | Journal of Genetics and Genomics2011，38，5： | 45 |
| 3 | GC-MS, GC-O and OAV analyses of key aroma compounds in Jiaozi Steamed Bread显示文摘Jiaozi Steamed Bread(JSB)has a unique aroma as a traditional staple food in China.The volatile compounds in JSBwere extracted by simultaneous distillation and extraction(SDE)and headspace solid-phasemicroextraction(HS-SPME).These volatile substances were analyzed by gas chromatography-mass spectrometry(GC-MS)and gas chromatographyolfactometry-mass spectrometry(GC-O-MS).The results demonstrated that 61 volatile compounds were identified totally in samples,of which 15 were confirmed as potent aroma compounds with odor active values(OAVs)>1.The 15 potent aroma compounds were ethanol,1-butanol,1-pentanol,1-hexanol,heptanol,1-octen-3-ol,3-methyl-1-butanol,hexanal,heptanal,nonanal,(E)-2-heptenal,benzaldehyde,(E,E)-2,4-decadienal,2-pentylfuran and naphthalene.The SDEmethod had better linearity with coefficients of determination(R2)equal to or higher than 0.9991.Furthermore,the SDE method also achieved lower sensitivity and better repeatability and recovery than HS-SPME.This work provides reference method and parameters for future research on the flavor of JSB for commercial products. | Yuanhui Wang Jingwen Zhao Fei Xu Xiaoyun Wu Wenxuan Hu Yongfeng Chang Lanlan Zhang Jie Chen Changhong Liu | 2020 | Grain & Oil Science and Technology2020，3，1： | 17 |
| 4 | Blocking FSH inhibits hepatic cholesterol biosynthesis and reduces serum cholesterol显示文摘Menopause is associated with dyslipidemia and an increased risk of cardio-cerebrovascular disease.The classic view assumes that the underlying mechanism of dyslipidemia is attributed to an insufficiency of estrogen.In addition to a decrease in estrogen, circulating follicle-stimulating hormone (FSH)levels become elevated at menopause.In this study,we find that blocking FSH reduces serum cholesterol via inhibiting hepatic cholesterol biosynthesis.First,epidemiological results show that the serum FSH levels are positively correlated with the serum total cholesterol levels,even after adjustment by considering the effects of serum estrogen,in addition,the prevalence of hypercholesterolemia is significantly higher in peri-menopausal women than that in premenopausal women.Furthermore,we generated a mouse model of FSH elevation by intraperitoneally injecting exogenous FSH into ovariectomized (OVX)mice,in which a normal level of estrogen (E2)was maintained by exogenous supplementation. Consistently,the results indicate that FSH,independent of estrogen,increases the serum cholesterol level in this mouse model. Moreover,blocking FSH signaling by anti-FSHβ antibody or ablating the FSH receptor (FSHR)gene could effectively prevent hypercholesterolemia induced by FSH injection or high-cholesterol diet feeding.Mechanistically,FSH,via binding to hepatic FSHRs, activates the Gi2α/β-arrestin-2/Akt pathway and subsequently inhibits the binding of FoxO1 with the SREBP-2 promoter,thus preventing FoxO1 from repressing SREBP-2 gene transcription.This effect,in turn,results in the upregulation of SREBP-2,which drives HMGCR nascent transcription and de novo cholesterol biosynthesis,leading to the increase of cholesterol accumulation.This study uncovers that blocking FSH signaling might be a new strategy for treating hypercholesterolemia during menopause, particularly for women in peri-menopause characterized by FSH elevation only. | Yanjing Guo Meng Zhao Tao Bo Shizhan Ma Zhongshang Yuan Wenbin Chen Zhao He Xu Hou Jun Liu Zhenhai Zhang Qiang Zhu Qiangxiu Wang Xiaoyan Lin Zhongli Yang Min Cui Lu Liu Yujie Li Chunxiao Yu Xiaoyi Qi Qian Wang Haiqing Zhang Qingbo Guan Lifang Zhao Shimeng Xuan Huili Yan Yanliang Lin Li Wang Qihang Li Yongfeng Song Ling Gao Jiajun Zhao | 2019 | Cell Research2019，29，2： | 16 |
| 5 | Tomato SlAN11 regulates flavonoid biosynthesis and seed dormancy by interaction with bHLH proteins but not with MYB proteins显示文摘The flavonoid compounds are important secondary metabolites with versatile human nutritive benefits and fulfill a multitude of functions during plant growth and development.The abundance of different flavonoid compounds are finely tuned with species-specific pattern by a ternary MBW complex,which consists of a MYB,a bHLH,and a WD40 protein,but the essential role of SlAN11,which is a WD40 protein,is not fully understood in tomato until now.In this study,a tomato WD40 protein named as SlAN11 was characterized as an effective transcription regulator to promote plant anthocyanin and seed proanthocyanidin(PA)contents,with late flavonoid biosynthetic genes activated in 35S::SlAN11 transgenic lines,while the dihydroflavonol flow to the accumulation of flavonols or their glycosylated derivatives was reduced by repressing the expression of SlFLS in this SlAN11-overexpressed lines.The above changes were reversed in 35S::SlAN11-RNAi transgenic lines except remained levels of flavonol compounds and SlFLS expression.Interestingly,our data revealed that SlAN11 gene could affect seed dormancy by regulating the expressions of abscisic acid(ABA)signaling-related genes SlABI3 and SlABI5,and the sensitivity to ABA treatment in seed germination is conversely changed by SlAN11-overexpressed or-downregulated lines.Yeast two-hybrid assays demonstrated that SlAN11 interacted with bHLH but not with MYB proteins in the ternary MBW complex,whereas bHLH interacted with MYB in tomato.Our results indicated that low level of anthocyanins in tomato fruits,with low expression of bHLH(SlTT8)and MYB(SlANT1 and SlAN2)genes,remain unchanged upon modification of SlAN11 gene alone in the transgenic lines.These results suggest that the tomato WD40 protein SlAN11,coordinating with bHLH and MYB proteins,plays a crucial role in the fine adjustment of the flavonoid biosynthesis and seed dormancy in tomato. | Yongfeng Gao Jikai Liu Yongfu Chen Hai Tang Yang Wang Yongmei He Yongbin Ou Xiaochun Sun Songhu Wang Yinan Yao | 2018 | Horticulture Research2018，5，1： | 13 |
| 6 | Effect of heat treatment on mechanical properties of heavily cold-rolled Fe-6.5wt%Si alloy sheet显示文摘Fe-6.5wt%Si alloy has excellent soft magnetic properties,but it is hard to be cold-rolled due to appearance of ordered phases in this alloy.In this paper we report that ultra thin Fe-6.5wt%Si sheet of 0.05 mm thick was obtained by heavily cold rolling.By means of optical microscope,micro-hardness indenter,instron,SEM and X-ray diffraction,the effect of heat treatment on mechanical properties of this alloy sheet was investigated.The heavily cold-rolled sheet exhibits some extent of ductility.The ultimate tensile strength reaches 1.93 GPa.After heat treatment,micro-hardness is decreased and the ductility is lost,especially at temperature above 650℃ when recrystallization takes place.The reason for decreasing the ductility lies in the ordered DO3 phase transformation. | LIANG YongFeng,YE Feng,LIN JunPin,WANG YanLi,ZHANG LaiQi & CHEN GuoLiang State Key Laboratory for Advanced Metals and Materials,University of Science and Technology Beijing,Beijing 100083,China | 2010 | Science China(Technological Sciences)2010，53，4： | 10 |
| 7 | Switchable C02 electroreduction via engineering active phases of Pd nanoparticles显示文摘活跃阶段的工程定期被利用在异构的催化作用调节金属 nanoparticles (NP ) 的选择。然而,在 electrocatalysis 的活跃阶段的理解的缺乏为公司 2 electroreduction 妨碍了有效催化剂的发展。此处,我们报导 Pd NP 的活跃阶段的系统的工程,它被利用为公司 2 electroreduction 选择反应小径。在 situ X光检查吸收光谱学,在稀释的 situ 总计思考红外线的光谱学,并且密度功能的理论计算建议吸附氢的 Pd 的形成出现在混合物上-并且一个钯氢化物核心的阶段(在 0.2 V 上面的+ PdH x @PdH x )(对一个可逆的氢电极)经由 HCOO 便于甲酸盐生产*中间,而阶段 Pd 氢化物核心上的金属性的 Pd 表面的形成( PdH x @主要产品,是甲酸盐或公司,能有选择地与高 Faradaic 效率被生产(>90%) 并且在有可伸缩的申请的 0.05 ~ 0.9 V 的潜在的窗户中的集体活动示范。 | Dunfeng Gao Hu Zhou Fan Cai Dongniu Wang Yongfeng Hu Bei Jiang Wen-Bin Cai Xiaoqi Chen Rui Si Fan Yang Shu Miao Jianguo Wang Guoxiong wang Xinhe Bao | 2017 | Nano Research2017，10，6： | 8 |
| 8 | 3-year Treatment of Tenofovir Alafenamide vs.Tenofovir Disoproxil Fumarate for Chronic HBV Infection in China显示文摘Background and Aims:Tenofovir alafenamide(TAF)has similar efficacy to tenofovir disoproxil fumarate(TDF)but with improved renal and bone safety in chronic hepatitis B patients studied outside of China.We report 3-year results from two phase 3 studies with TAF in China(Clinicaltrials.gov:NCT02836249 and NCT02836236).Methods:Chinese hepatitis B e antigen(HBeAg)-positive and-negative chronic hepatitis B patients with viremia and elevated alanine aminotransferase were randomized 2:1 to TAF or TDF treatment groups and treated in a double-blind fashion for 144 weeks(3 years).Efficacy responses were assessed by individual study while safety was assessed by a pooled analysis.Results:Of the 334 patients(180 HBeAg-positive and 154 HBeAg-negative)randomized and treated,baseline characteristics were similar between groups.The overall mean age was 38 years and 73%were male.The mean HBV DNA was 6.4 log10 IU/mL.The median alanine aminotransferase was 88 U/L,and 37%had a history of antiviral use.At week 144,the proportion with HBV DNA<29 IU/mL was similar among the two groups,with TAF at 83%vs.TDF at 79%,and TAF at 93%vs.TDF at 92%for the HBeAg-positive and-negative patients,respectively.In each study,higher proportions of TAF than TDF patients showed normalized alanine aminotransferase(via the American Association for the Study of Liver Diseases and the China criteria)and showed loss of HBsAg;meanwhile,the HBeAg seroconversion rates were similar.Treatment was well-tolerated among the TAF patients,who showed a smaller median decline in creatinine clearance(−0.4 vs.−3.2 mL/min;p=0.014)and less percentage change in bone mineral density vs.TDF at hip(−0.95%vs.−1.93%)and spine(+0.35%vs.−1.40%).Conclusions:In chronic hepatitis B patients from China,TAF treatment provided efficacy similar to TDF but with better renal and bone safety at 3 years. | Jinlin Hou Qin Ning Zhongping Duan You Chen Qing Xie Fu-Sheng Wang Lunli Zhang Shanming Wu Hong Tang Jun Li Feng Lin Yongfeng Yang Guozhong Gong John FFlaherty Anuj Gaggar Shuyuan Mo Cong Cheng Gregory Camus Chengwei Chen Yan Huang Jidong Jia Mingxiang Zhang GS-US-320-0110 and GS-US-320-0108 China Investigators | 2021 | Journal of Clinical and Translational Hepatology2021，9，3： | 7 |
| 9 | Sedimentary Evolution of the Qinghai-Tibet Plateau in Cenozoic and its Response to the Uplift of the Plateau显示文摘We have studied the evolution of the tectonic lithofacies paleogeography of Paleocene-Eocene, Oligocene, Miocene, and Pliocene of the Qinghai-Tibet Plateau by compiling data regarding the type, tectonic setting, and lithostratigraphic sequence of 98 remnant basins in the plateau area. Our results can be summarized as follows. (1) The Paleocene to Eocene is characterized by uplift and erosion in the Songpan-Garzê and Gangdisê belts, depression (lakes and pluvial plains) in eastern Tarim, Qaidam, Qiangtang, and Hoh Xil, and the Neo-Tethys Sea in the western and southern Qinghai-Tibet Plateau. (2) The Oligocene is characterized by uplift in the Gangdisê-Himalaya and Karakorum regions (marked by the absence of sedimentation), fluvial transport (originating eastward and flowing westward) in the Brahmaputra region (marked by the deposition of Dazhuka conglomerate), uplift and erosion in western Kunlun and Songpan-Garzê, and depression (lakes) in the Tarim, Qaidam, Qiangtang, and Hoh Xil. The Oligocene is further characterized by depressional littoral and neritic basins in southwestern Tarim, with marine facies deposition ceasing at the end of the Oligocene. (3) For the Miocene, a widespread regional unconformity (ca. 23 Ma) in and adjacent to the plateau indicates comprehensive uplift of the plateau. This period is characterized by depressions (lakes) in the Tarim, Qaidam, Xining-Nanzhou, Qiangtang, and Hoh Xil. Lacustrine facies deposition expanded to peak in and adjacent to the plateau ca. 18-13 Ma, and north-south fault basins formed in southern Tibet ca. 13-10 Ma. All of these features indicate that the plateau uplifted to its peak and began to collapse. (4) Uplift and erosion occurred during the Pliocene in most parts of the plateau, except in the Hoh Xil-Qiangtang, Tarim, and Qaidam. The continuous uplift and intensive taphrogeny in the plateau divided the original large basin into small basins, deposition of lacustrine facies decreased considerably, and boulderstone accumulated, indicating a response to the overall uplift of the plateau. Here, we discuss the evolution of tectonic lithofacies paleogeography in Cenozoic and its response to the tectonic uplift of the Qinghai-Tibet Plateau in relation to the above characteristics. We have recognized five major uplift events, which occurred during 58-53 Ma, 45-30 Ma, 25-20 Ma, 13-7 Ma, and since 5 Ma. The results presented here indicate that the paleogeomorphic configurations of the Qinghai-Tibet Plateau turned over during the late Miocene, with high elevations in the east during the pre-Miocene switching to high contours in the west at the end of Miocene. | ZHANG Kexin WANG Guocan XU Yadong LUO Mansheng JI Junliang XIAO Guoqiao WANG An SONG Bowen LIANG Yinpin JIANG Shangsong CAO Kai CHEN Fenning CHEN Ruiming YANG Yongfeng | 2013 | Acta Geologica Sinica(English Edition)2013，87，2： | 6 |
| 10 | Key Distribution over the Based on Covert Communication VoIP显示文摘 | HUANG Yongfeng YUAN Jian CHEN Minchao XIAO Bo | 2011 | Chinese Journal of Electronics2011，20，2： | 5 |
| 11 | Up-regulation of glycolipid transfer protein by bicyclol causes spontaneous restriction of hepatitis C virus replication显示文摘Bicyclol is a synthetic drug for hepatoprotection in clinic since 2004. Preliminary clinical observations suggest that bicyclol might be active against hepatitis C virus(HCV) with unknown mechanism. Here, we showed that bicyclol significantly inhibited HCV replication in vitro and in hepatitis C patients. Using bicyclol as a probe, we identified glycolipid transfer protein(GLTP) to be a novel restrictive factor for HCV replication. The GLTP preferentially bound host vesicle-associated membrane protein-associated protein-A(VAP-A) in competition with the HCV NS5 A, causing an interruption of the complex formation between VAP-A and HCV NS5 A. As the formation of VAP-A/NS5 A complex is essential for viral RNA replication, up-regulation of GLTP by bicyclol reduced the level of VAP-A/NS5 A complex and thus inhibited HCV replication. Bicyclol also exhibited an inhibition on HCV variants resistant to direct-acting antiviral agents(DAAs) with an efficacy identical to that on wild type HCV. In combination with bicyclol, DAAs inhibited HCV replication in a synergistic fashion. GLTP appears to be a newly discovered host restrictive factor for HCV replication, Up-regulation of GLTP causes spontaneous restriction of HCV replication. | Meng-Hao Huang Hu Li Rong Xue Jianrui Li Lihua Wang Junjun Cheng Zhouyi Wu Wenjing Li Jinhua Chen Xiaoqin Lv Qiang Li Pei Lan Limin Zhao Yongfeng Yang Zonggen Peng Jiandong Jiang | 2019 | Acta Pharmaceutica Sinica B2019，9，4： | 3 |
| 12 | Cloning and Expression of a Chitinase Gene from Serratia marcescens Strain C8-8显示文摘A 1692 bp long chitinase-encoding chiA gene was cloned from the genomic DNA of Serratia marcescens strain C8-8 by PCR, which was speculated to encode a 563 aa long polypeptide chain with molecular weight of about 60.9 kD.Homology analysis showed that the chiA gene sequence cloned from C8-8 shared the highest similarity with chiA sequences from Serratia marcescens strains 141 (DQ 990373.1) and 14041 (DQ 493896.1), which reached 99%.Domain analysis showed that N-terminal (23 aa) of the chiA gene cloned from C8-8 harbored typical signal peptide sequence, while C-terminal harbored the other two domains, including the PKD region (73 aa) and chitinase catalytic region (387 aa).The PCR fragment was digested with restriction endonucleases and cloned into plasmid pET28a.The recombinant plasmid pET28a-chiA was firstly transformed into Escherichia coli DH5, and then transformed into expression host E.coli DH3 to express chiA gene.The recombinant strain DH3 chiA could produce transparent hydrolysis circles on the colloidal chitin plate induced by isopropyl-1-thiogalactopyranoside (IPTG).SDS-PAGE electrophoresis analysis showed that, a protein with relative molecular weight of about 60 kD was expressed by the recombinant strain DH3 chiA, which was consistent with the except molecular weight.After initial purification, biological activity test showed that the recombinant expression product could hydrolyze chitin, which produced transparent hydrolysis circles on the colloidal chitin plates.Results indicated that chiA gene from Serratia marcescens strain C8-8 had biological functions and could be utilized as a potential biological control factor. | Youzhou LIU Chuping LUO Yongfeng LIU Zhiyi CHEN | 2013 | Agricultural Biotechnology2013，2，3： | 3 |
| 13 | Porous Carbon Architecture Assembled by Cross-Linked Carbon Leaves with Implanted Atomic Cobalt for High-Performance Li-S Batteries显示文摘The practical application of lithium-sulfur batteries is severely hampered by the poor conductivity,polysulfide shuttle effect and sluggish reaction kinetics of sulfur cathodes.Herein,a hierarchi-cally porous three-dimension(3D)carbon architecture assembled by cross-linked carbon leaves with implanted atomic Co-N4 has been deli-cately developed as an advanced sulfur host through a SiO_(2)-mediated zeolitic imidazolate framework-L(ZIF-L)strategy.The unique 3D architectures not only provide a highly conductive network for fast electron transfer and buffer the volume change upon lithiation-delithi-ation process but also endow rich interface with full exposure of Co-N4 active sites to boost the lithium polysulfides adsorption and conversion.Owing to the accelerated kinetics and suppressed shuttle effect,the as-prepared sulfur cathode exhibits a superior electrochemical perfor-mance with a high reversible specific capacity of 695 mAh g^(−1) at 5 C and a low capacity fading rate of 0.053%per cycle over 500 cycles at 1 C.This work may provide a promising solution for the design of an advanced sulfur-based cathode toward high-performance Li-S batteries. | Ruirui Wang Renbing Wu Chaofan Ding Ziliang Chen Hongbin Xu Yongfeng Liu Jichao Zhang Yuan Ha Ben Fei Hongge Pan | 2021 | Nano-Micro Letters2021，13，10： | 3 |
| 14 | Conductive photo-thermal responsive bifunctional hydrogel system with self-actuating and self-monitoring abilities显示文摘Despite enormous efforts in actuators,most researches are only limited to various actuation behaviors and demonstrations of soft materials.It has not yet been reported to capture and monitor its movement status in an invisible environment.Therefore,it is of great significance to develop a self-sensing and self-actuating dual-function hydrogel actuator system to realize real-time monitoring.Here,we report a bifunctional hydrogel system with self-actuating and self-monitoring abilities,which combines the functions of photothermal actuation and electrical resistance sensing into a single material.The bilayer tough conductive hydrogel synthesized by unconventional complementary concentration recombination and cryogenic freezing technique presents a dense conductive network and high-porosity structure,achieving high toughness at 190.3 kPa of tensile strength,high stretchability(164.3%strain),and the toughness dramatically(1,471.4 kJ·m^(−3)).The working mechanism of the monitoring and self-sensing system is accomplished through the integrated monitoring device of surface temperature–bending angle–electron current,to solve the problem of not apperceiving actuator motion state when encountering obstacles in an invisible environment.We demonstrated for the first time a photothermal actuator’s motion of a football player and goalkeeper to finish the penalty and a soft actuator hand,which can achieve the action of sticking to grab and release under photo-thermal actuation.When connected to the control closed circuit,the actuator realized closed-loop monitoring and sensing feedback.The development of bifunctional hydrogel systems may bring new opportunities and ideas in the fields of material science,circuit technology,sensors,and mechanical engineering. | Neng Chen Yang Zhou Yinping Liu Yuanyuan Mi Sisi Zhao Wang Yang Sai Che Hongchen liu FengJiang Chen Chong Xu Guang Ma Xue Peng Yongfeng Li | 2022 | Nano Research2022，15，8： | 3 |
| 15 | First intron of nestin gene regulates its expression during C2C12 myoblast differentiation显示文摘Nestin 是在神经祖先房间并且在锻炼骨胳的肌肉表示的中间的细丝蛋白质。Nestin 广泛地被用作一个神经祖先房间标记。在在神经祖先房间的基因的巢的特定的表示被在基因位于巢的第二内含子的神经特定的增强子授与,这很好被建立。然而,在在锻炼肌肉的表示的巢的 transcriptional 机制仍然是不清楚的。在这研究,我们识别了肌肉在在在老鼠 myoblast C2C12 房间的基因的老鼠巢的第一内含子的房间特定的增强子。我们局部性核心增强子活动到第一内含子的 291-661 区域,并且证明在核心增强子区域的二电子盒子为在区分 C2C12 房间的增强子活动是重要的。我们也证明那 MyoD 蛋白质在 C2C12 房间的 myogenic 区别在表示涉及巢的规定。 | Hua Zhong Zhigang Jin Yongfeng Chen Ting Zhang Wei Bian Xing Cui Naihe Jing | 2008 | Acta Biochimica et Biophysica Sinica2008，40，6： | 3 |
| 16 | Particle swarm optimization and its application to seismic inversion of igneous rocks显示文摘In order to improve the fine structure inversion ability of igneous rocks for the exploration of underlying strata, based on particle swarm optimization(PSO), we have developed a method for seismic wave impedance inversion. Through numerical simulation, we tested the effects of different algorithm parameters and different model parameterization methods on PSO wave impedance inversion, and analyzed the characteristics of PSO method. Under the conclusions drawn from numerical simulation, we propose the scheme of combining a cross-moving strategy based on a divided block model and high-frequency filtering technology for PSO inversion. By analyzing the inversion results of a wedge model of a pitchout coal seam and a coal coking model with igneous rock intrusion, we discuss the vertical and horizontal resolution, stability and reliability of PSO inversion. Based on the actual seismic and logging data from an igneous area, by taking a seismic profile through wells as an example, we discuss the characteristics of three inversion methods, including model-based wave impedance inversion, multi-attribute seismic inversion based on probabilistic neural network(PNN) and wave impedance inversion based on PSO.And we draw the conclusion that the inversion based on PSO method has a better result for this igneous area. | Yang Haijun Xu Yongzhong Peng Gengxin Yu Guiping Chen Meng Duan Wensheng Zhu Yongfeng Cui Yongfu Wang Xingjun | 2017 | International Journal of Mining Science and Technology2017，27，2： | 3 |
| 17 | Observations of upper layer turbulent mixing in the southern South China Sea显示文摘A turbulent microstructure experiment was undertaken at a low latitude of 10°N in the South China Sea in late August 2012. The characteristics of the eddy diffusivity above 650 m were analyzed, which is one order of magnitude larger than that in the open ocean at that low latitude. Enhanced eddy diffusivities by strong shears and sharp changes in topography were observed. The strongest eddy diffusivity occurred in the mixed layer, and it reached O(10–2 m2/s). Strong stratification in the thermocline inhibited the penetration of surface eddy diffusivities through the thermocline, where the mixing was weakest. Below the thermocline, where the background eddy diffusivity was approximately O(10–6 m2/s), the eddy diffusivity increased with depth, and its largest value was O(10–3 m2/s). | SHANG Xiaodong QI Yongfeng CHEN Guiying LIANG Changrong | 2015 | Acta Oceanologica Sinica2015，34，11： | 3 |
| 18 | Regulation of Leaf Longevity by DML3-Mediated DNA Demethylation显示文摘Leaf senescence is driven by the expression of senescence-associated genes(SAGs).Development-specific genes often undergo DNA demethylation in their promoter and other regions,which regulates gene expression.Whether and how DNA demethylation regulates the expression of SAGs and thus leaf senescence remain elusive.Whole-genome bisulfite sequencing(WGBS)analyses of wild-type(WT)and demeter-like 3(dml3)Arabidopsis leaves at three developmental stages revealed hypermethylation during leaf senescence in dml3 compared with WT,and 20556 differentially methylated regions(DMRs)were identified by comparing the methylomes of dml3 and WT in the CG,CHG,and CHH contexts.Furthermore,we identified that 335 DMR-associated genes(DMGs),such as NAC016 and SEN1,are upregulated during leaf senescence,and found an inverse correlation between the DNA methylation levels(especially in the promoter regions)and the transcript abundances of the related SAGs in WT.In contrast,in dml3 the promoters of SAGs were hypermethylated and their transcript levels were remarkably reduced,and leaf senescence was significantly delayed.Collectively,our study unraveled a novel epigenetic regulatory mechanism underlying leaf senescence in which DML3 is expressed at the onset of and during senescence to demethylate promoter,gene body or 3'UTR regions to activate a set of SAGs. | Lu Yuan Dan Wang Liwen Cao Ningning Yu Ke Liu Yongfeng Guo Susheng Gan Liping Chen | 2020 | Molecular Plant2020，13，8： | 3 |
| 19 | Investigating the trip configured causal effect of distracted driving on aggressive driving behavior for e-hailing taxi drivers显示文摘Risky driving behavior of taxi drivers typically evaluated for full operation or sometimes sorted into occupied and empty running trips.In this paper,we simultaneously analyze aggressive driving and distracted driving of taxi drivers under three different trip categories.Trip origin is considered a transition from without ride-order to with ride-order travelling or from with ride-order to occupied travelling,and a destination as a transition from occupied to without ride-order travelling and vice versa.Distracted driving is characterized by driver interference,driver mobile use and some entertainment aspects,while specific harmful and risky actions are considered for aggressive driving.High-resolution and real-time kinematic parameters of taxis were recorded by the in-vehicle recorder VBOX for overall 562 trips.The distracted driving parameters and aggressive driving actions were monitored through python data collector web application that was specially programmed for this particular research.Besides dual dash cam(i.e.,front and inside car camera),drivers’ whole day driving history from their Chinese ride-hailing Di Di smart application was used to differentiate occupied trips,unoccupied trips with ride-order and unoccupied trips without ride-order.Structural equation modeling(SEM) is practiced in this paper to understand the influence of distracted driving indicators on aggressive driving behaviors.The multi-group model analysis of SEM indicated that handling distracted risky driving could control aggressive driving behavior up to 96% and 98% inunoccupied without ride-order trips and unoccupied trips with ride-order respectively.The model has also identified the sensitive risky driving indicators for each group separately. | Muhammad Sajjad Ansar Yongfeng Ma Shuyan Chen Kun Tang Ziyu Zhang | 2021 | Journal of Traffic and Transportation Engineering(English Edition)2021，8，5： | 3 |
| 20 | Bacisubin, an antifungal protein with ribonuclease and hemagglutinating activities from Bacillus subtilis strain B-916显示文摘 | Yongfeng Liu Zhiyi Chen T.B. Ng Jie Zhang Mingguo Zhou Fuping Song Fan Lu Youzhou Liu | 2006 | Peptides2006，，3： | 3 |
您所在的机构暂未开通此部分的功能，请联系管理员