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| 1 | 2018 Chinese Pediatric Cardiology Society(CPCS) guideline for diagnosis and treatment of syncope in children and adolescents显示文摘Syncope belongs to the transient loss of consciousness(TLOC), characterized by a rapid onset, short duration, and spontaneous complete recovery. It is common in children and adolescents, accounting for 1% to 2% of emergency department visits.Recurrent syncope can seriously affect children's physical and mental health, learning ability and quality of life and sometimes cardiac syncope even poses a risk of sudden death. The present guideline for the diagnosis and treatment of syncope in children and adolescents was developed for guiding a better clinical management of pediatric syncope. Based on the globally recent development and the evidence-based data in China, 2018 Chinese Pediatric Cardiology Society(CPCS) guideline for diagnosis and treatment of syncope in children and adolescents was jointly prepared by the Pediatric Cardiology Society, Chinese Pediatric Society, Chinese Medical Association(CMA)/Committee on Pediatric Syncope, Pediatricians Branch, Chinese Medical Doctor Association(CMDA)/Committee on Pediatric Cardiology, Chinese College of Cardiovascular Physicians, Chinese Medical Doctor Association(CMDA)/Pediatric Cardiology Society, Beijing Pediatric Society, Beijing Medical Association(BMA). The present guideline includes the underlying diseases of syncope in children and adolescents, the diagnostic procedures, methodology and clinical significance of standing test and headup tilt test, the clinical diagnosis vasovagal syncope, postural orthostatic tachycardia syndrome, orthostatic hypotension and orthostatic hypertension, and the treatment of syncope as well as follow-up. | Cheng Wang Yaqi Li Ying Liao Hong Tian Min Huang Xiangyu Dong Lin Shi Jinghui Sun Hongfang Jin Junbao Du Jindou An Jie Chen Mingwu Chen Qi Chen Sun Chen Yonghong Chen Zhi Chen Adolphus Kai-tung Chau Junbao Du Zhongdong Du Junkai Duan Hongyu Duan Xiangyu Dong Lin Feng Lijun Fu Fangqi Gong Yonghao Gui Ling Han Zhenhui Han Bing He Zhixu He Xiufen Hu Yimin Hua Guoying Huang Min Huang Ping Huang Yujuan Huang Hongfang Jin Mei Jin Bo Li Fen Li Tao Li Xiaohui Li Xiaoyan Liu Yan Li Haitao Lv Tiewei Lv Zipu Li Luyi Ma Silin Pan Yusheng Pang Hua Peng Yuming Qin Jie Shen Lin Shi Kun Sun Jinghui Sun Hong Tian Jie Tian Cheng Wang Hong Wang Lei Wang Jinju Wang Wendi Wang Yuli Wang Rongzhou Wu Tianhe Xia Yanyan Xiao Chunhong Xie Yanlin Xing Zhenyu Xiong Baoyuan Xu Yi Xu Hui Yan Shiwei Yang Qijian Yi Xia Yu Xianyi Yu Yue Yuan Hongyan Zhang Huili Zhang Li Zhang Qingyou Zhang Xi Zhang Yanmin Zhang Zhiwei Zhang Cuifen Zhao Bin Zhou Hua Zhu | 2018 | Science Bulletin2018，63，23： | 54 |
| 2 | Selective laser melting 3D printing of Ni-based superalloy: understanding thermodynamic mechanisms显示文摘A mesoscopic model has been established to investigate the thermodynamic mechanisms and densification behavior of nickel-based superalloy during additive manufacturing/three-dimensional(3D) printing(AM/3DP)by numerical simulation, using a finite volume method(FVM). The influence of the applied linear energy density(LED) on dimensions of the molten pool, thermodynamic mechanisms within the pool, bubbles migration and resultant densification behavior of AM/3DP-processed superalloy has been discussed. It reveals that the center of the molten pool slightly shifts with a lagging of 4 lm towards the center of the moving laser beam. The Marangoni convection, which has various flow patterns, plays a crucial role in intensifying the convective heat and mass transfer, which is responsible for the bubbles migration and densification behavior of AM/3DP-processed parts. At an optimized LED of 221.5 J/m, the outward convection favors the numerous bubbles to escape from the molten pool easily and the resultant considerably high relative density of 98.9 % is achieved. However, as the applied LED further increases over 249.5 J/m, the convection pattern is apparently intensified with the formation of vortexes and the bubbles tend to be entrapped by the rotating flow within the molten pool, resulting in a large amount of residual porosity and a sharp reduction in densification of the superalloy. The change rules of the relative density and the corresponding distribution of porosity obtained by experiments are in accordance with the simulation results. | Mujian Xia Dongdong Gu Guanqun Yu Donghua Dai Hongyu Chen Qimin Shi | 2016 | Science Bulletin2016，61，13： | 13 |
| 3 | Microstructure and Oxidation Behaviors of Nano-particles Strengthened NiCoCrAlY Cladded Coatings on Superalloys显示文摘Nano-particles which can largely improve the microstructure and oxidation resistance of materials are often used as a strengthening component in metal matrix composites. However, few studies were reported on its application in the bond coat of duplex structure thermal barrier coating(TBC). Three kinds of NiCoCrAlY coatings strengthened by different nano-particles with the same addition (1%, mass fraction) were prepared by the laser cladding technique on Ni-based superalloy substrates, aiming to study the effects of the nano-particles on microstructure and oxidation resistance of NiCoCrAlY coatings (the bond coat of the duplex structure thermal barrier coatings). Scanning electron microscope (SEM), X-ray diffractometer(XRD) and thermogravimetry were employed to investigate their morphologies, phases and cyclic oxidation behaviors in atmosphere at 1 050℃, compared with the coating without nano-particles. With the addition of nano-particles, the growth pattern of the grains at the interface changed from epitaxial growth to non-epitaxial growth or part-epitaxial growth; slender dendrites were broken and cellularized; cracks and pores were restrained; and the oxidation weight-gain and the stripping resistance of the oxide scale were improved as well. Among the three kinds of nano-particles, the SiC nano-particles showed the most improvement on microstructure, while the CeO2 nano-particles were insufficient, but its effects on the oxidation resistance are the same as those of the SiC nano-particles. Based on the discussions of the influence mechanism, it is believed that CeO2 nano-particles would show better improvement than SiC nano-particles if the proper amount is added and the proper preparation technique of micro-nanometer composite powders is adopted, with the synergistic action of nanometer effect and reactive element effect. | WANG Hongyu ZUO Dunwen CHEN Xinfeng YU Shouxin GU Yuanzhi | 2010 | Chinese Journal of Mechanical Engineering2010，23，3： | 11 |
| 4 | A Domestication-Associated Gene GmPRR3b Regulates the Circadian Clock and Flowering Time in Soybean显示文摘Improved soybean cultivars have been adapted to grow at a wide range of latitudes,enabling expansion of cultivation worldwide.However,the genetic basis of this broad adaptation is still not clear.Here,we report the identification of GmPRR3b as a major flowering time regulatory gene that has been selected during domestication and genetic improvement for geographic expansion.Through a genome-wide association study of a diverse soybean landrace panel consisting of 279 accessions,we identified 16 candidate quantitative loci associated with flowering time and maturity time.The strongest signal resides in the known flowering gene E2,verifying the effectiveness of our approach.We detected strong signals associated with both flowering and maturity time in a genomic region containing GmPRR3b.Haplotype analysis revealed that GmPRR3bH6 is the major form of GmPRR3b that has been utilized during recent breeding of modern cultivars.mRNA profiling analysis showed that GmPRR3bH6 displays rhythmic and photoperiod-dependent expression and is preferentially induced under long-day conditions.Overexpression of GmPRR3bH6 increased main stem node number and yield,while knockout of GmPRR3bH6 using CRISPR/Cas9 technology delayed growth and the floral transition.GmPRR3bH6 appears to act as a transcriptional repressor of multiple predicted circadian clock genes,including GmCCAIa,which directly upregulates J/GmELF3a to modulate flowering time.The causal SNP(Chr12:5520945)likely endows GmPRR3bH6 a moderate but appropriate level of activity,leading to early flowering and vigorous growth traits preferentially selected during broad adaptation of landraces and improvement of cultivars. | Cong Li Ying-hui Li Yanfei Li Hongfeng Lu Huilong Hong Yu Tian Hongyu Li Tao Zhao Xiaowei Zhou Jun Liu Xinan Zhou Scott A.Jackson Bin Liu Li-juan Qiu | 2020 | Molecular Plant2020，13，5： | 11 |
| 5 | The Cambrian sedimentary characteristics and their implications for oil and gas exploration in north margin of Middle-Upper Yangtze Plate显示文摘Using data from tens of measured and observed outcrop successions,thin rock slices and sample analyses,we comprehensively studied the Cambrian sedimentary environments and evolutionary characteristics in the north margin of the Middle-Upper Yangtze Plate.During the Cambrian,platform,slope,and deep sea basin environments were developed in the study area.On the platform,both clastic rocks and carbonate rocks were deposited.Clastic rocks mainly occur in the Lower Cambrian,and were deposited in marine shore and shelf environments.Carbonate rocks are dominant in the Middle and Upper Cambrian,and were deposited in the open platform,restricted platform,tidal flat,beach,and reef environments.Carbonate gravity flow deposits were developed on the slope.In the basin,mainly black shales and chert beds were deposited.The Cambrian represents one large transgression-regression cycle,and maximum transgression occurred in the Qiongzhusi Age of the Early Cambrian.Tectonics and sea level fluctuations had important impacts on sedimentary environments.The Chengkou-Fangxian-Xiangfan Fracture controlled the position of the platform,slope and basin,as well as the silica supply for chert deposition in basin.Sea level fluctuations controlled types of sediments and sedimentary facies on the platform.In the study area,there are good reservoir rocks,including dolomites,grainstones,debris flow deposits,sandstones,and conglomerates;there are good source rocks,including black shales,dark micrites,and chert beds;and there are also good reservoir-source rock assemblages.The hydrocarbon potential of the study area is great. | YU KuanHong JIN ZhenKui SU Kui DONG XiaoDong ZHANG Wei DU HongYu CHEN Ying ZHANG WeiDan | 2013 | Science China Earth Sciences2013，56，6： | 9 |
| 6 | Laser-driven programmable non-contact transfer printing of objects onto arbitrary receivers via an active elastomeric microstructured stamp显示文摘Transfer printing, as an important assembly technique, has attracted much attention due to its valuable merits to develop novel forms of electronics such as stretchable inorganic electronics requiring the heterogeneous integration of inorganic materials with soft elastomers. Here, we report on a laser-driven programmable non-contact transfer printing technique via a simple yet robust design of active elastomeric microstructured stamp that features cavities filled with air and embedded under the contacting surface, a micro-patterned surface membrane that encapsulates the air cavities and a metal layer on the inner-cavity surfaces serving as the laser-absorbing layer. The micro-patterned surface membrane can be inflated dynamically to control the interfacial adhesion, which can be switched from strong state to weak state by more than three orders of magnitude by local laser heating of the air in the cavity with a temperature increase below 100℃. Theoretical and experimental studies reveal the fundamental aspects of the design and fabrication of the active elastomeric microstructured stamp and the operation of non-contact transfer printing. Demonstrations in the programmable transfer printing of micro-scale silicon platelets and micro-scale LED chips onto various challenging receivers illustrate the extraordinary capabilities for deterministic assembly that are difficult to address by existing printing schemes, thereby creating engineering opportunities in areas requiring the heterogeneous integration of diverse materials such as curvilinear electronics and Micro LED displays. | Hongyu Luo Chengjun Wang Changhong Linghu Kaixin Yu Chao Wang Jizhou Song | 2020 | National Science Review2020，7，2： | 9 |
| 7 | Reversible and irreversible heat generation of NCA/Si–C pouch cell during electrochemical energy-storage process显示文摘To meet the requirements of electronic vehicles(EVs) and hybrid electric vehicles(HEVs),the high energy density Li Ni_(0.8) Co_(0.15) Al_(0.05) O_2(NCA) cathode and Si–C anode have attracted more attention.Here we report the thermal behaviors of NCA/Si–C pouch cell during the charge/discharge processes at different current densities.The total heat generations are derived from the surface temperature change during electrochemical Li+insertion/extraction in adiabatic surrounding.The reversible heat is determined by the entropic coefficients,which are related with open-circuit voltage at different temperatures; while the irreversible heat is determined by the internal resistance,which can be obtained via V–I characteristic,electrochemical impedance spectroscopy and hybrid pulse power characterization(HPPC).During the electrochemical process,the reversible heat contributes less than 10% to total heat generation; and the heat generated in charge process is less than that in discharge process.The results of thermal behaviors analyses are conducive to understanding the safety management and paving the way for building a reliable thermal model of high energy density lithium ion battery. | Ying Bai Limin Li Yu Li Guanghai Chen Huichun Zhao Zhaohua Wang Chuan Wu Hongyun Ma Xinquan Wang Hongyue Cui Jiang Zhou | 2019 | Journal of Energy Chemistry2019，28，2： | 7 |
| 8 | Combination Patterns and Depositional Characteristics of Ordovician Carbonate Banks in the Western Tarim Basin,China显示文摘碳酸盐银行的联合模式和 depositional 特征在西方的 Tarim 盆基于奥陶纪的露头节,薄节,和碳同位素被调查,中国。四个碳酸盐银行联合模式在奥陶纪被扔,西方的 Tarim 盆,包括:礁石银行建筑群(RBC ) , Algae-Reef-Bank Interbed (ARBI ) ,厚层的蛋糕沉积银行(TLCAB ) ,和薄层的蛋糕逆行银行(TLCRB ) 。所有联合模式垂直地显示出清楚的时期。RBC 主要由礁石和 bioclastic 银行组成,并且 RBC 的尺寸取决于礁石的规模。Bioclastic 银行存款包围礁石。ARBI 的范围被水藻礁石,水藻 peloid 白云石 microfacies 和垂直地轮流出现的海藻的白云石 microfacies 沉积物的规模决定。当在周期叠并且与生气床上用品广泛地延长的层蛋糕发展了, TLCAB 和 TLCRB 被扔。TLCAB 和 TLCRB 的谷物是多样的并且多来源。与影响相对的海水平变化,生物发展和地形学, ARBI, TLCAB 或 TLCRB, RBC 连续地从更低的奥陶纪 Penglaiba 形成被开发到中间的奥陶纪 Yijianfang 形成。奥陶纪的 depositional 环境分析显示 RBC 和 ARBI 能形成有效的油和煤气的水库,并且 TLCAB 和 TLCRB 有潜力形成巨大的规模油和煤气的水库并且以后是为奥陶纪碳酸盐银行的探索的关键目标。 | HU Xiaolan FAN Tailiang GAO Zhiqian YU Bingsong CHEN Xiaozhi WANG Hongyu WANG Jianping LI Rangbin DING Meng LI Yifan | 2012 | Acta Geologica Sinica(English Edition)2012，86，4： | 7 |
| 9 | A new distinct geminivirus causes soybean stay-green disease显示文摘Dear Editor,Plant viruses make up almost half of the plant disease-causing pathogens,affecting crop yields and the global economy(Savary et al.,2019).Soybean(Glycine max)is one of the most valuable legume crops in the world,supplying 25%of the global edible oil and two-thirds of the global concentrated protein for livestock feeding.Recently,the outbreak of soybean stay-green syndrome with delayed leaf senescence(stay-green),flat pods,and increased number of abnormal seeds has swept the soybean production in the Huang-Huai-Hai region of China,resulting in huge yield losses(Xu et al.,2019).This disease has become an epidemic and prominent problem in soybean production and is still expanding its geography,including North America,posing a serious threat to soybean production(Harbach et al.,2016;Zhang et al.,2016;Li et al.,2019).However,the cause of soybean stay-green syndrome remains obscure. | Ruixiang Cheng Ruoxin Mei Rong Yan Hongyu Chen Dan Miao Lina Cai Jiayi Fan Gairu Li Ran Xu Weiguo Lu Yu Gao Wenwu Ye Shuo Su Tianfu Han Junyi Gai Yuanchao Wang Xiaorong Tao Yi Xu | 2022 | Molecular Plant2022，15，6： | 6 |
| 10 | Effects of carbon diffusing field in alloy solvent on the growth of tower-shape diamond single crystal显示文摘High-quality type-Ib tower-shape diamond single crystals were synthesized in cubic anvil high pressure apparatus (SPD-6×1200) at 5.4 GPa and 1250-1450°C. The (100) face of seed crystal was used as the growth face, and FeNiMnCo alloy was used as the solvent/catalyst. Two kinds of carbon diffusing fields (type-B and type-G) were simulated by finite element method (FEM). Using the two kinds of carbon diffusing fields, many diamond single crystals were synthesized. The effects of carbon diffusing fields on the crystal quality and β value (the ratio of height to diameter of diamond crystal) were studied. The results show that using type-B diffusing field, considerable inclusions appeared in tower-shape diamond crystals with high β values; however, using type- G diffusing field, inclusions were reduced markedly in high β values tower-shape diamond crystals, and the crystal, up to 0.6 carat in weight and 5.3 mm in size, was synthesized. Experimental phenomena were explained well with the help of the FEM. | XIAO HongYu MA HongAn TIAN Yu LI Rui LI ShangSheng MA LiQiu LI Yong JIA XiaoPeng | 2010 | Chinese Science Bulletin2010，55，1： | 5 |
| 11 | Pain threshold, anxiety and other factors affect intensity of postoperative pain in gastric cancer patients: A prospective cohort study显示文摘Objective: This prospective cohort study explored factors related to postoperative pain in gastric cancer patients.Methods: A total of 236 patients who underwent gastrectomy were enrolled. All patients enrolled in the study completed the Hospital Anxiety and Depression Scale(HADS) questionnaire and Life Orientation Test-Revised(LOT-R) questionnaire on the day before surgery. Heat pain threshold(HPT), cold pain threshold(CPT) and pressure pain threshold(PPT) were measured for all patients one day prior to surgery and demographic details were collected. All patients were connected to a patient-controlled intravenous analgesia(PCIA) pump at the end of the surgery. The occurrence of postoperative pain was used as a dependent variable, and multivariate logistic regression analyses were conducted to screen for factors affecting postoperative pain.Results: In total, 83 patients(35.2%) had postoperative pain. Body mass index(BMI) ≥28 kg/m^(2) [odds ratio(OR): 2.67;95% confidence interval(95% CI): 1.07-6.67], total gastrectomy(OR: 2.64;95% CI: 1.42-4.91),preoperative anxiety score ≥8(OR: 2.37;95% CI: 1.12-5.02), heat pain threshold ≤4.9 s(OR: 2.14;95% CI:1.06-4.32), pressure pain threshold ≤4 g(OR: 2.05;95% CI: 1.05-4.03), and female gender(OR: 1.99;95% CI:1.04-3.83) were risk factors for postoperative pain.Conclusions: Obesity, wide range of gastrectomy, high preoperative anxiety, low HPT and PPT, and female gender are associated with increased risk for postoperative pain. | Hongyu Tan Jin Wei Shuo Li Ling Yu Hongwei Sun Ke Ji Yinkui Wang Changlong Li | 2021 | Chinese Journal of Cancer Research2021，33，3： | 5 |
| 12 | Dependence of nitrogen concentration in type Ib diamonds on synthesis temperature显示文摘Type Ib diamonds were grown by the temperature gradient method (TGM) at 5.5 GPa and 1500-1560 K in a china-type cubic anvil high pressure apparatus using Ni70Mn25Co5 alloy as solvent/catalyst. The concentration of nitrogen (CN) in type Ib diamonds synthesized at different synthesis temperatures was measured by a Fourier transform infrared (FTIR) spectrometer. The dependence of CN in diamond on synthesis temperature was studied. For the type Ib diamonds synthesized using Ni70Mn25Co5 as catalyst, its CN decreases along with the increase of synthesis temperature. | TIAN Yu JIA XiaoPeng ZANG ChuanYi LI ShangSheng XIAO HongYu ZHANG YaFei HUANG GuoFeng LI Rui HAN QiGang MA LiQiu LI Yong CHEN XiaoZhou ZHANG Chong MA HongAn | 2009 | Chinese Science Bulletin2009，54，9： | 5 |
| 13 | Propulsion Principles of Water Striders in Sculling Forward through Shadow Method显示文摘 | Hongyu Lu Yelong Zheng Wei Yin Dashuai Tao Noshir Pesika Yonggang Meng Yu Tian | 2018 | Journal of Bionic Engineering2018，15，3： | 5 |
| 14 | A Comparison in Laser Precision Drilling of Stainless Steel 304 with Nanosecond and Picosecond Laser Pulses显示文摘Precision drilling with picosecond laser has been advocated to significantly improve the quality of micro-holes with reduced recast layer thickness and almost no heat affected zone.However,a detailed comparison between nanosecond and picosecond laser drilling techniques has rarely been reported in previous research.In the present study,a series of micro-holes are manufactured on stainless steel 304 using a nanosecond and a picosecond laser drilling system,respectively.The quality of the micro-holes,e.g.,recast layer,micro-crack,circularity,and conicity,etc,is evaluated by employing an optical microscope,an optical interferometer,and a scanning electron microscope.Additionally,the micro-structure of the samples between the edges of the micro-holes and the parent material is compared following etching treatment.The researching results show that a great amount of spattering material accumulated at the entrance ends of the nanosecond laser drilled micro-holes.The formation of a recast layer with a thickness of^25μm is detected on the side walls,associated with initiation of micro-cracks.Tapering phenomenon is also observed and the circularity of the micro-holes is rather poor.With regard to the micro-holes drilled by picosecond laser,the entrance ends,the exit ends,and the side walls are quite smooth without accumulation of spattering material,formation of recast layer and micro-cracks.The circularity of the micro-holes is fairly good without observation of tapering phenomenon.Furthermore,there is no obvious difference as for the micro-structure between the edges of the micro-holes and the parent material.This study proposes a picosecond laser helical drilling technique which can be used for effective manufacturing of high quality micro-holes. | ZHANG Hongyu DI Jianke ZHOU Ming YAN Yu | 2014 | Chinese Journal of Mechanical Engineering2014，27，5： | 5 |
| 15 | Luminescence anti-counterfeiting:From elementary to advanced显示文摘Luminescence anti-counterfeiting derives from the easily changeable luminescence behaviors of luminescence materials under the regulation of various external stimuli(such as excitation light,chemical reagent,heat,and mechanical force,etc.)and luminescence lifetime,which plays an important role in preventing forgery of currency,artworks,and product brands.According to the numbers of changes of anti-counterfeiting labels under various regulation conditions,luminescence anti-counterfeiting can be classified into three levels from elementary to advanced:single-level anti-counterfeiting,double-level anti-counterfeiting,and multilevel anti-counterfeiting.In this review,the recent achievements in luminescence anti-counterfeiting are summarized,and the regulation of various factors to anti-counterfeiting labels is discussed.Finally,existing problems,future challenges,and possible development directions are proposed in order to realize facile,quick,low-cost,environmentally friendly,and difficult-to-replicate advanced luminescence anti-counterfeiting. | Xiaowei Yu Hongyue Zhang Jihong Yu | 2021 | Aggregate2021，2，1： | 4 |
| 16 | Clonogenic colony-forming ability of hepatic stem cells in the spleens of mice显示文摘To confirm the existence of hepatic stem cells (HSCs), fetal liver cells isolated from mice on embryonic day 13 (ED13) were long-term cultured in vitro. Growth of the cells was observed intensively and characteristics were iden-tified by immunocytochemistry. The results showed that some of the cells grew as colonies, in which some cells ex-pressed AFP, CD34 and Albumin. Then the cells were trans-planted intravenously into irradiated syngeneic mice. At day 12 a number of small hyperplasia nodules were seen in the apparently enlarged spleens of recipient mice. Moreover, some nodules were positive for AFP and CD34 and consisted of various types of cells, suggesting the very existence of he-patic stem cells in the mouse fetal liver. | SU Juan YAO Yucheng YU Hongyu ZHU Haiyinq WANG Xinmin WANG Zhonghua HU Yiping | 2002 | Chinese Science Bulletin2002，47，3： | 4 |
| 17 | Application of regional citrate anticoagulation in patients at high risk of bleeding during intermittent hemodialysis:a prospective multicenter randomized controlled trial显示文摘Objective:Safe and effective anticoagulation is essential for hemodialysis patients who are at high risk of bleeding.The purpose of this trial is to evaluate the effectiveness and safety of two-stage regional citrate anticoagulation(RCA)combined with sequential anticoagulation and standard calcium-containing dialysate in intermittent hemodialysis(IHD)treatment.Methods:Patients at high risk of bleeding who underwent IHD from September 2019 to May 2021 were prospectively enrolled in 13 blood purification centers of nephrology departments,and were randomly divided into RCA group and saline flushing group.In the RCA group,0.04 g/mL sodium citrate was infused from the start of the dialysis line during blood draining and at the venous expansion chamber.The sodium citrate was stopped after 3 h of dialysis,which was changed to sequential dialysis without anticoagulant.The hazard ratios for coagulation were according to baseline.Results:A total of 159 patients and 208 sessions were enrolled,including RCA group(80 patients,110 sessions)and saline flushing group(79 patients,98 sessions).The incidence of severe coagulation events of extracorporeal circulation in the RCA group was significantly lower than that in the saline flushing group(3.64%vs.20.41%,P<0.001).The survival time of the filter pipeline in the RCA group was significantly longer than that in the saline flushing group((238.34±9.33)min vs.(221.73±34.10)min,P<0.001).The urea clearance index(Kt/V)in the RCA group was similar to that in the saline flushing group with no statistically significant difference(1.12±0.34 vs.1.08±0.34,P=0.41).Conclusions:Compared with saline flushing,the two-stage RCA combined with a sequential anticoagulation strategy significantly reduced extracorporeal circulation clotting events and prolonged the dialysis time without serious adverse events. | Xiaoyan TANG Dezheng CHEN Ling ZHANG Ping FU Yanxia CHEN Zhou XIAO Xiangcheng XIAO Weisheng PENG Li CHENG Yanmin ZHANG Hongbo LI Kehui LI Bizhen GOU Xin WU Qian YU Lijun JIAN Zaizhi ZHU Yu WEN Cheng LIU Hen XUE Hongyu ZHANG Xin HE Bin YAN Liping ZHONG Bin HUANG Mingying MAO | 2022 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2022，23，11： | 4 |
| 18 | HBB-deficient Macaca fascicularis monkey presents with human β-thalassemia显示文摘Dear Editor,β-Thalassemia is a common severe genetic disease caused by mutations in HBB and affects approximately 1.5% of the global population (Origa, 2017). In southern China, the carrier rate of β-thalassemia is as high as 6.43%, creating a high socio-economic burden (Xiong et al., 2010). In adult humans, there are three types of hemoglobin: HbA1 (~97%), HbA2 (~2%) and HbF (~1%). HbA1 (α2β2) is composed of two a-globin and two β-globi n sub units en coded by HBA and HBB, respectively;HbF (α2β2)is made up of two α-globin subunits and two β-globin sub units en coded by HBG. Mutations in the coding region or regulatory region of HBB are involved in β-thalassemia pathogenesis. Except for some rare dominant mutations, most HBB mutations are recessive (Origa, 2017). Depending on the mutation type, the β-globin level will either be reduced or completely depleted, resulting in α-globin accumulation and precipitation. These α-globin precipitates lead to red blood cell death, resulting in anemia and tissue damage, and even death in thalassemia major patients. Blood transfusions can help slow disease progression but lead to iron overload, ultimately resulting in iron toxicity. Bone marrow transfer is the only cure in the clinic and is available only to a small percentage of patients with human leukocyte antigervmatched donors. Recently, gene therapy and gene editing therapy have shown great promise in curing β-thalassemia (Glaser et al., 2015;Thompson et al., 2018). However, no appropriate animal models are available for evaluating the safety and efficacy of such advanced therapeutic strategies in vivo.β-thalassemia mice are the sole animal model available for research. However, substantial differences have been reported between the types and expressi on patter ns of human and mouse globins (McColl and Vadolas, 2016). Moreover, mice contain no fetal globin gene equivalent, and homozygous mutations of HBB in mouse for early models of β-thalassemia major or Cooley anemia are all embryonic lethal (Huo et al., 2009). Recently, significant phenotype and physiology differences have been reported between SIRT6- null mice and the non-human primate model (Zhang et al., 2018). Thus, an appropriate non-human primate model is needed for human β-thalassemia studies and treatments. | Yan Huang Chenhui Ding Puping Liang Duanduan Li Yu Tang Wei Meng Hongwei Sun Hongyu Lu Yu Chen Xueying Chen Qunshan Huang Jianpei Fang Canquan Zhou Shihua Yang Junjiu Huang | 2019 | Protein & Cell2019，10，7： | 4 |
| 19 | Automatic segmentation of foveal avascular zone based on adaptive watershed algorithm in retinal optical coherence tomography angiography images显示文摘The size and shape of the foveal avascular zone(FAZ)have a strong positive correlation with several vision-threatening ret inovascular diseases.The identification,segmentation and analysis of FAZ are of great significance to clinical diagnosis and treatment.We presented an adaptive watershed algorithm to automatically extract F AZ from retinal optical coherence tomography angiography(OCTA)images.For the traditional watershed algorithm,'over-segmentation'is the most common problem.FAZ is often incorrectly divided into multiple regions by redundant'dams'.This paper analyzed the relationship between the'dams'length and the maximum inscribed circle radius of FAZ,and proposed an adaptive watershed algorithm to solve the problem of'over-segmentation'.Here,132 healthy retinal images and 50 diabetic retinopathy(DR)images were used to verify the accuracy and stability of the algorithm.Three ophthal-mologists were invited to make quan titative and qualitative evaluations on the segmentation results of this algorithm.The quantitative evaluation results show that the correlation coffi-cients between the automatic and manual segmentation results are 0.945(in healthy subjects)and 0.927(in DR patients),respectively.For qualitative evaluation,the percentages of'perfect segmentation'(score of 3)and'good segmentation'(score of 2)are 99.4%(in healthy subjects)and 98.7%(in DR patients),respectively.This work promotes the application of watershed algorithm in FAZ segmentation,making it a useful tool for analyzing and diagnosing eye diseases. | Jian Liu Shixin Yan Nan Lu Dongni Yang Chunhui Fan Hongyu Lv Shuanglian Wang in Zhu Yuqian Zhao Yi Wang Zhenhe Ma Yao Yu | 2022 | Journal of Innovative Optical Health Sciences2022，15，1： | 3 |
| 20 | A stringent dual control system overseeing transcription and activity of the Cre recombinase for the liver-specific conditional gene knock-out mouse model显示文摘Liver cancer is one of the most threatening diseases in Chinese population. Just like in other tissues, tumor initiation and development in liver involve multiple steps of genetic and epigenetic alterations with several unknown details. However, unlike in other tissues, a tis-sue specific inducible Cre recombinase system that allows temporal and spatial deletion of a target DNA fragment is still not available for in vivo functional gene annotation in hepatocytes. In our pursuit to establish such a mouse model, we designed a dual inducible Cre transgene system and tested it in cultured cells. By combining a CCAAT/enhancer binding protein β (C/EBP β) promoter derived Tet-off expression system and the estrogen receptor (ER) mediated functional control, we show a desirable profile of both hepatocyte-specificity and regulability of the Cre expression in a series of critical assessments in the cell culture system, which provides confidence in continua-tion of our ongoing pursuit in mouse. | Yu Wu Yinghua He Hongyu Zhang Xinlan Dai Xiaoyu Zhou Jun Gu Guan Wang Jingde Zhu | 2008 | Journal of Genetics and Genomics2008，35，7： | 3 |
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