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| 1 | Humoral immune response to circulating SARS-CoV-2 variants elicited by inactivated and RBD-subunit vaccines显示文摘SARS-CoV-2 variants could induce immune escape by mutations on the receptor-binding domain(RBD)and N-terminal domain(NTD).Here we report the humoral immune response to circulating SARS-CoV-2 variants,such as 501Y.V2(B.1.351),of the plasma and neutralizing antibodies(NAbs)elicited by CoronaVac(inactivated vaccine),ZF2001(RBD-subunit vaccine)and natural infection.Among 86 potent NAbs identified by high-throughput single-cell VDJ sequencing of peripheral blood mononuclear cells from vaccinees and convalescents,near half anti-RBD NAbs showed major neutralization reductions against the K417N/E484K/N501Y mutation combination,with E484K being the dominant cause.VH3-53/VH3-66 recurrent antibodies respond differently to RBD variants,and K417N compromises the majority of neutralizing activity through reduced polar contacts with complementarity determining regions.In contrast,the 242–244 deletion(242–244Δ)would abolish most neutralization activity of anti-NTD NAbs by interrupting the conformation of NTD antigenic supersite,indicating a much less diversity of anti-NTD NAbs than anti-RBD NAbs.Plasma of convalescents and CoronaVac vaccinees displayed comparable neutralization reductions against pseudo-and authentic 501Y.V2 variants,mainly caused by E484K/N501Y and 242–244Δ,with the effects being additive.Importantly,RBD-subunit vaccinees exhibit markedly higher tolerance to 501Y.V2 than convalescents,since the elicited anti-RBD NAbs display a high diversity and are unaffected by NTD mutations.Moreover,an extended gap between the third and second doses of ZF2001 leads to better neutralizing activity and tolerance to 501Y.V2 than the standard three-dose administration.Together,these results suggest that the deployment of RBD-vaccines,through a third-dose boost,may be ideal for combating SARS-CoV-2 variants when necessary,especially for those carrying mutations that disrupt the NTD supersite. | Yunlong Cao Ayijiang Yisimayi Yali Bai Weijin Huang Xiaofeng Li Zhiying Zhang Tianjiao Yuan Ran An Jing Wang Tianhe Xiao Shuo Du Wenping Ma Liyang Song Yongzheng Li Xiang Li Weiliang Song Jiajing Wu Shuo Liu Xuemei Li Yonghong Zhang Bin Su Xianghua Guo Yangyang Wei Chuanping Gao Nana Zhang Yifei Zhang Yang Dou Xiaoyu Xu Rui Shi Bai Lu Ronghua Jin Yingmin Ma Chengfeng Qin Youchun Wang Yingmei Feng Junyu Xiao Xiaoliang Sunney Xie | 2021 | Cell Research2021，31，7： | 12 |
| 2 | Safety and superior immunogenicity of heterologous boosting with an RBD-based SARS-CoV-2 mRNA vaccine in Chinese adults显示文摘Dear Editor,To May 2022,the COVID-19 pandemic has claimed more than 6.28 million lives,with more than 524 million confirmed cases worldwide.The recent emergence of highly transmissible Omicron variant of severe acute respiratory syndrome coronavirus 2(SARSCoV-2)has triggered another major surge in both confirmed cases and deaths.1 Ten COVID-19 vaccines have been approved by the World Health Organization(WHO)for emergency use,including the two mRNA vaccines,BNT162b2 and mRNA-1273,and two Chinese inactivated vaccines,CoronaVac and BBIBP-CorV.However,the rapid waning of vaccine-induced virus-neutralizing antibody titers and the continuous emergence of variants of concern(VOCs),including Alpha,Beta,Delta and Omicron,have created unprecedented challenges in the eradication of COVID-19 pandemic.2–4. | Xiaoqiang Liu Yuhua Li Zhongfang Wang Shouchun Cao Weijin Huang Lin Yuan Yi-Jiao Huang Yan Zheng Jingjing Chen Bo Ying Zuoyun Xiang Jin Shi Jincun Zhao Zhen Huang Cheng-Feng Qin | 2022 | Cell Research2022，32，8： | 5 |
| 3 | A novel STING agonist-adjuvanted pan-sarbecovirus vaccine elicits potent and durable neutralizing antibody and T cell responses in mice,rabbits and NHPs显示文摘The emergence of SARS-CoV-2 variants and potentially other highly pathogenic sarbecoviruses in the future highlights the need for pan-sarbecovirus vaccines.Here,we discovered a new STING agonist,CF501,and found that CF501-adjuvanted RBD-FC vaccine(CF501/RBD-FC)elicited significantly stronger neutralizing antibody(nAb)and T cell responses than Alum-and cGAMP-adjuvanted RBD-FC in mice.Vaccination of rabbits and rhesus macaques(nonhuman primates,NHPs)with CF501/RBD-FC elicited exceptionally potent nAb responses against SARS-CoV-2 and its nine variants and 41 S-mutants,SARS-CoV and bat SARSr-CoVs.CF501/RBD-FC-immunized hACE2-transgenic mice were almost completely protected against SARS-CoV-2 challenge,even 6 months after the initial immunization.NHPs immunized with a single dose of CF501/RBD-FC produced high titers of nAbs.The immunized macaques also exhibited durable humoral and cellular immune responses and showed remarkably reduced viral load in the upper and lower airways upon SARS-CoV-2 challenge even at 108 days post the final immunization.Thus,CF501/RBD-Fc can be further developed as a novel pan-sarbecovirus vaccine to combat current and future outbreaks of sarbecovirus diseases. | Zezhong Liu Jie Zhou Wei Xu Wei Deng Yanqun Wang Meiyu Wang Qian Wang Ming Hsieh Jingming Dong Xinling Wang Weijin Huang Lixiao Xing Miaoling He Chunlin Tao Youhua Xie Yilong Zhang Youchun Wang Jincun Zhao Zhenghong Yuan Chuan Qin Shibo Jiang Lu Lu | 2022 | Cell Research2022，32，3： | 5 |
| 4 | Immunogenicity and protective efficacy of a recombinant protein subunit vaccine and an inactivated vaccine against SARS-CoV-2 variants in non-human primates显示文摘Emerging SARS-CoV-2 variants and the gradually decreasing neutralizing antibodies over time post vaccination have led to an increase in incidents of breakthrough infection across the world.To investigate the potential protective effect of the recombinant protein subunit COVID-19 vaccine targeting receptor-binding domain(RBD)(PS-RBD)and whole inactivated virus particle vaccine(IV)against the variant strains,in this study,rhesus macaques were immunized with PS-RBD or IV vaccine,followed by a Beta variant(B.1.351)challenge.Although neutralizing activity against the Beta variant was reduced compared with that against the prototype,the decreased viral load in both upper and lower respiratory tracts,milder pathological changes,and downregulated inflammatory cytokine levels in lung tissues after challenge demonstrated that PS-RBD and IV still provided effective protection against the Beta variant in the macaque model.Furthermore,PS-RBD-induced macaque sera possessed general binding and neutralizing activity to Alpha,Beta,Delta,and Omicron variants in our study,though the neutralizing antibody(NAb)titers declined by varying degrees,demonstrating potential protection of PS-RBD against current circulating variants of concern(VOCs).Interestingly,although the IV vaccine-induced extremely low neutralizing antibody titers against the Beta variant,it still showed reduction for viral load and significantly alleviated pathological change.Other correlates of vaccine-induced protection(CoP)like antibody-dependent cellular cytotoxicity(ADCC)and immune memory were both confirmed to be existing in IV vaccinated group and possibly be involved in the protective mechanism. | Qian He Qunying Mao Xiaozhong Peng Zhanlong He Shuaiyao Lu Jialu Zhang Fan Gao Lianlian Bian Chaoqiang An Wenhai Yu Fengmei Yang Yanan Zhou Yun Yang Yanyan Li Yadi Yuan Xujia Yan Jinghuan Yang Xing Wu Weijin Huang Changgui Li Junzhi Wang Zhenglun Liang Miao Xu | 2022 | Signal Transduction and Targeted Therapy2022，7，4： | 2 |
| 5 | In-situ weldalloying plasma arc welding of SiCp/Al MMCs 显示文摘 | Lei Yueheng Yuan Weijin Chen Xizhang | 2007 | Transactions Nonferrous Metals Society China2007，17，2： | 1 |
| 6 | Controlling polar-toroidal multi-order states in twisted ferroelectric nanowires显示文摘The toroidal order of electric dipoles in ferroelectric materials has attracted attention in the past decade due to fascinating properties and great potential for enabling novel memory devices,and functional devices in general.However,facile manipulation of toroidal order in ferroelectrics remains challenging.Here,using first-principles derived simulations,we demonstrate an efficient scheme to control the polar-toroidal multi-order(PTMO)states in ferroelectric nanowires.Two feasible strategies of controlling PTMO states by a combination of homogeneous electric field and torque are carried out in ferroelectric/paraelectric composite nanowires.This is possible based on trilinear coupling between polarization,toroidization and the twist force.As a result,switching of the toroidization of the nanowire can be readily achieved by reversal of the axial polarization.The torque threshold needed to control PTMO states is also calculated and found to be relatively small,indicating the feasibility of this method.Our study demonstrates facile control of PTMO states,including ferroelectric skyrmions,in ferroelectrics and is a step towards designing ferroelectric devices based on multi-order states. | Jianyi Liu Ye Ji Shuai Yuan Lili Ding Weijin Chen Yue Zheng | 2018 | npj Computational Materials2018，，1： | 0 |
| 7 | Machine learning predicting and engineering the yield,N content,and specific surface area of biochar derived from pyrolysis of biomass显示文摘Biochar produced from pyrolysis of biomass has been developed as a platform carbonaceous material that can be used in various applications.The specific surface area(SSA)and functionalities such as N-containing functional groups of biochar are the most significant properties determining the application performance of biochar as a carbon material in various areas,such as removal of pollutants,adsorption of CO_(2)and H2,catalysis,and energy storage.Producing biochar with preferable SSA and N functional groups is among the frontiers to engineer biochar materials.This study attempted to build machine learning models to predict and optimize specific surface area of biochar(SSA-char),N content of biochar(N-char),and yield of biochar(Yield-char)individually or simultaneously,by using elemental,proximate,and biochemical compositions of biomass and pyrolysis conditions as input variables.The predictions of Yield-char,N-char,and SSA-char were compared by using random forest(RF)and gradient boosting regression(GBR)models.GBR outperformed RF for most predictions.When input parameters included elemental and proximate compositions as well as pyrolysis conditions,the test R^(2) values for the single-target and multi-target GBR models were 0.90-0.95 except for the two-target prediction of Yield-char and SSA-char which had a test R^(2) of 0.84 and the three-target prediction model which had a test R^(2) of 0.81.As indicated by the Pearson correlation coefficient between variables and the feature importance of these GBR models,the top influencing factors toward predicting three targets were specified as follows:pyrolysis temperature,residence time,and fixed carbon for Yield-char;N and ash for N-char;ash and pyrolysis temperature for SSA-char.The effects of these parameters on three targets were different,but the trade-offs of these three were balanced during multi-target ML prediction and optimization.The optimum solutions were then experimentally verified,which opens a new way for designing smart biochar with target properties and oriented application potential. | Lijian Leng Lihong Yang Xinni Lei Weijin Zhang Zejian Ai Zequn Yang Hao Zhan Jianping Yang Xingzhong Yuan Haoyi Peng Hailong Li | 2022 | Biochar2022，4，1： | 0 |
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