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| 1 | Reversal of B-cell hyperactivation and functional impairment is associated with HBsAg seroconversion in chronic hepatitis B patients显示文摘B 房间对再感染在肝炎 B 病毒(HBV ) 和保护的清理起一个重要作用。然而,与长期的 HBV 感染的结果被联系的这些房间的功能的特征仍然保持未知。我们包括地在不同阶段从 CHB 病人调查了外部 B 房间子集的频率,显型,和功能:有免疫力的忍耐(信息技术) ,有免疫力的激活(IA ) ,有免疫力的清理(IC ) ,有 HBsAg seroconversion 的应答者(解决的病人, RP ) ,并且健康控制(HC ) 。IA 病人与另外的组相比显示了外部血记忆 B 房间的更低的百分比。B 房间的全面 polyclonal 激活,由激活标记和 IgG 和 IgM 的分泌物的高水平显示了,在 IA 病人被观察。这 B 房间 hyperactivation 能被增加的 IFN-α 导致;并且在 IA 病人的可溶的 CD40 ligands。尤其是,合作激发器分子 CD80 和浆液 HBsAb 的表示和 HBsAg 特定的 B 房间的频率显著地与 HC 题目相比在信息技术, IA,和 IC 病人被减少。更重要地, B 房间 hyperactivation, co-stimulatory 分子 downregulation 和 HBsAg 特定的 B 房间缺陷在 RP 病人被颠倒。B 房间 hyperactivation 和功能的缺陷的颠倒在长期的肝炎 B 病人与 HBsAg seroconversion 被联系。 | Xiangsheng Xu Qinghua Shang Xinyue Chen Weimin Nie Zhengsheng Zou Ang Huang Ming Meng Lei Jin Ruonan Xu Ji-Yuan Zhang Junliang Fu Lifeng Wang Zirong Tang Yunbo Xie Xiaoming Yang Zheng Zhang Fu-Sheng Wang | 2015 | Cellular & Molecular Immunology2015，12，3： | 15 |
| 2 | MicroRNAs in tomato plants显示文摘MicroRNAs (miRNAs) are a specialized class of small silencing RNAs that regulate gene expression in eukaryotes.In plants,miRNAs negatively regulate target mRNAs containing a highly complementary sequence by either mRNA cleavage or translational repression.As a model plant to study fleshy fruit ripening,miRNA studies in tomato have made great progress recently.MiRNAs were predicted to be involved in nearly all biological processes in tomato,particularly development,differentiation,and biotic and abiotic stress responses.Surprisingly,several miRNAs were verified to be involved in tomato fruit ripening and senescence.Recent studies suggest that miRNAs are related to host-virus interactions,which raises the possibility that miRNAs can be used as diagnostic markers for response to virus infection in tomato plants.In this review,we summarize our current knowledge systematically and advance future directions for miRNA research in tomato. | ZUO JinHua WANG YunXiang LIU HaiPing MA YuanZheng JU Zheng ZHAI BaiQiang FU DaQi ZHU Yi LUO YunBo ZHU BenZhong | 2011 | Science China(Life Sciences)2011，54，7： | 13 |
| 3 | Virus-induced Gene Silencing in Eggplant(Solanum melongena)显示文摘Eggplant(Solanum melongena)is an economically important vegetable requiring investigation into its various genomic functions.The current limitation in the investigation of genomic function in eggplant is the lack of effective tools available for conducting functional assays.Virus-induced gene silencing(VIGS)has played a critical role in the functional genetic analyses.In this paper,TRV-mediated VIGS was successfully elicited in eggplant.We first cloned the CDS sequence of PDS(PHYTOENE DESATURASE) in eggplant and then silenced the PDS gene.Photo-bleaching was shown on the newly-developed leaves four weeks after agroinoculation,indicating that VIGS can be used to silence genes in eggplant.To further illustrate the reliability of VIGS in eggplant,we selected Chl H,Su and CLA1 as reporters to elicit VIGS using the high-pressure spray method.Suppression of Chl H and Su led to yellow leaves,while the depletion of CLA1 resulted in albino.In conclusion,four genes,PDS,Chl H,Su(Sulfur),CLA1,were down-regulated significantly by VIGS,indicating that the VIGS system can be successfully applied in eggplant and is a reliable tool for the study of gene function. | Haiping Liu Daqi Fu Benzhong Zhu Huaxue Yan Xiaoying Shen Jinhua Zuo Yi Zhu Yunbo Luo | 2012 | Journal of Integrative Plant Biology2012，54，6： | 11 |
| 4 | A NAC transcription factor, NOR-like1, is a new positive regulator of tomato fruit ripening显示文摘Ripening of the model fruit tomato(Solanum lycopersicum)is controlled by a transcription factor network including NAC(NAM,ATAF1/2,and CUC2)domain proteins such as No-ripening(NOR),SlNAC1,and SlNAC4,but very little is known about the NAC targets or how they regulate ripening.Here,we conducted a systematic search of fruit-expressed NAC genes and showed that silencing NOR-like1(Solyc07g063420)using virus-induced gene silencing(VIGS)inhibited specific aspects of ripening.Ripening initiation was delayed by 14 days when NOR-like1 function was inactivated by CRISPR/Cas9 and fruits showed obviously reduced ethylene production,retarded softening and chlorophyll loss,and reduced lycopene accumulation.RNA-sequencing profiling and gene promoter analysis suggested that genes involved in ethylene biosynthesis(SlACS2,SlACS4),color formation(SlGgpps2,SlSGR1),and cell wall metabolism(SlPG2a,SlPL,SlCEL2,and SlEXP1)are direct targets of NOR-like1.Electrophoretic mobility shift assays(EMSA),chromatin immunoprecipitation-quantitative PCR(ChIP-qPCR),and dual-luciferase reporter assay(DLR)confirmed that NOR-like1 bound to the promoters of these genes both in vitro and in vivo,and activated their expression.Our findings demonstrate that NOR-like1 is a new positive regulator of tomato fruit ripening,with an important role in the transcriptional regulatory network. | Ying Gao Wei Wei Xiaodan Zhao Xiaoli Tan Zhongqi Fan Yiping Zhang Yuan Jing Lanhuan Meng Benzhong Zhu Hongliang Zhu Jianye Chen Cai-Zhong Jiang Donald Grierson Yunbo Luo Da-Qi Fu | 2018 | Horticulture Research2018，5，1： | 9 |
| 5 | Diversity and redundancy of the ripening regulatory networks revealed by the fruitENCODE and the new CRISPR/Cas9 CNR and NOR mutants显示文摘Tomato is considered as the genetic model for climacteric fruits,in which three major players control the fruit ripening process:ethylene,ripening transcription factors,and DNA methylation.The fruitENCODE project has now shown that there are multiple transcriptional circuits regulating fruit ripening in different species,and H3K27me3,instead of DNA methylation,plays a conserved role in restricting these ripening pathways.In addition,the function of the core tomato ripening transcription factors is now being questioned.We have employed CRISPR/Cas9 genome editing to mutate the SBP-CNR and NAC-NOR transcription factors,both of which are considered as master regulators in the current tomato ripening model.These plants only displayed delayed or partial non-ripening phenotypes,distinct from the original mutant plants,which categorically failed to ripen,suggesting that they might be gain-of-function mutants.Besides increased DNA methylation genome-wide,the original mutants also have hyper-H3K27me3 in ripening gene loci such as ACS2,RIN,and TDR4.It is most likely that multiple genetic and epigenetic factors have contributed to their strong non-ripening phenotypes.Hence,we propose that the field should move beyond these linear and twodimensional models and embrace the fact that important biological processes such as ripening are often regulated by highly redundant network with inputs from multiple levels. | Ying Gao Ning Zhu Xiaofang Zhu Meng Wu Cai-Zhong Jiang Donald Grierson Yunbo Luo Wei Shen Silin Zhong Da-Qi Fu Guiqin Qu | 2019 | Horticulture Research2019，6，1： | 7 |
| 6 | Tomato DCL2b is required for the biosynthesis of 22-nt small RNAs, the resulting secondary siRNAs, and the host defense against ToMV显示文摘The tomato encode four functional DCL families,of which DCL2 is poorly studied.Here,we generated loss-of-function mutants for a tomato DCL2 gene,dcl2b,and we identified its major role in defending against tomato mosaic virus in relation to both natural and manual infections.Genome-wide small RNA expression profiling revealed that DCL2b was required for the processing 22-nt small RNAs,including a few species of miRNAs.Interestingly,these DCL2b-dependent 22-nt miRNAs functioned similarly to the DCL1-produced 22-nt miRNAs in Arabidopsis and could serve as triggers to generate a class of secondary siRNAs.In particular,the majority of secondary siRNAs were derived from plant defense genes when the plants were challenged with viruses.We also examined differentially expressed genes in dcl2b through RNA-seq and observed that numerous genes were associated with mitochondrial metabolism and hormone signaling under virus-free conditions.Notably,when the loss-of-function dcl2b mutant was challenged with tomato mosaic virus,a group of defense response genes was activated,whereas the genes related to lipid metabolism were suppressed.Together,our findings provided new insights into the roles of tomato DCL2b in small RNA biogenesis and in antiviral defense. | Tian Wang Zhiqi Deng Xi Zhang Hongzheng Wang Yu Wang Xiuying Liu Songyu Liu Feng Xu Tao Li Daqi Fu Benzhong Zhu Yunbo Luo Hongliang Zhu | 2018 | Horticulture Research2018，5，1： | 4 |
| 7 | The Observation of Ice-Nucleating Particles Active at Temperatures above –15℃ and Its Implication on Ice Formation in Clouds显示文摘A series of measurements of ice-nucleating particles(INPs) were performed at two sites in Beijing. At the Beijing Meteorological Service(BMS) site, which was an urban site, no INPs were found to be active above –15℃.However, at the Yanjiaping(YJP) site, which was a rural site, the concentration of INPs active at temperatures above–15℃ was found to be as high as 1.73 g^(–1). Two parameterizations were constructed by respectively fitting the data obtained at BMS site and YJP site. The two parameterizations, as well as another parameterization from the literature,were implemented into a parcel model to investigate the effect of INPs active above –15℃ on phase partitioning in mixed-phase clouds. At a vertical velocity of 0.01 m s^(–1), which is typical for stratiform clouds associated with frontal systems, the INPs active above –15℃ nucleate ice crystals at low levels. The growth of these ice crystals remarkably reduces both the maximum liquid water mixing ratio and the altitude where the maximum liquid water mixing ratio is reached. When the vertical velocity of the parcel is increased to 0.1 m s^(–1) or an even higher value, the evolution of liquid water mixing ratio is not controlled by the INPs active above –15℃ but those active below –15℃. | Kai BI Xincheng MA Yunbo CHEN Shizuo FU Huiwen XUE | 2018 | Journal of Meteorological Research2018，32，5： | 3 |
| 8 | Deciphering Ascorbic Acid Regulatory Pathways in Ripening Tomato Fruit Using a Weighted Gene Correlation Network Analysis Approach显示文摘Genotype is generally determined by the co-expression of diverse genes and multiple regulatory pathways in plants.Gene co-expression analysis combining with physiological trait data provides very important information about the gene function and regulatory mechanism.L-Ascorbic acid(AsA),which is an essential nutrient component for human health and plant metabolism,plays key roles in diverse biological processes such as cell cycle,cell expansion,stress resistance,hormone synthesis,and signaling.Here,we applied a weighted gene correlation network analysis approach based on gene expression values and AsA content data in ripening tomato(Solanum lycopersicum L.)fruit with different AsA content levels,which leads to identifcation of AsA relevant modules and vital genes in AsA regulatory pathways.Twenty-four modules were compartmentalized according to gene expression profling.Among these modules,one negatively related module containing genes involved in redox processes and one positively related module enriched with genes involved in AsA biosynthetic and recycling pathways were further analyzed.The present work herein indicates that redox pathways as well as hormone-signal pathways are closely correlated with AsA accumulation in ripening tomato fruit,and allowed us to prioritize candidate genes for follow-up studies to dissect this interplay at the biochemical and molecular level. | Chao Gao Zheng Ju Shan Li Jinhua Zuo Daqi Fu Huiqin Tian Yunbo Luo Benzhong Zhu | 2013 | Journal of Integrative Plant Biology2013，55，11： | 3 |
| 9 | Involvement of post?transcriptional regulation of FOXO1 by HuR in 5?FU?inducedapoptosis in breast cancer cells显示文摘 | Yunbo Li Jinhai Yu Danhua Du Shuanglin Fu Ye Chen Fang Yu Peng Gao | 2013 | Oncology Letters2013，，1： | 2 |
| 10 | Green Luminescent Center in Undoped Zinc Oxide Films Deposited on Silicon Substrates显示文摘 | Lin Bixia Fu Zhuxi Jia Yunbo | 2001 | Appl Phys Lett2001，79，： | 1 |
| 11 | Changes of Fecal Bifidobacterium Species in Adult Patients with Hepatitis B Virus-Induced Chronic Liver Disease显示文摘 | Min Xu Baohong Wang Yiqi Fu Yanfei Chen Fengling Yang Haifeng Lu Yunbo Chen Jiali Xu Lanjuan Li | 2012 | Microbial Ecology2012，，2： | 1 |
| 12 | Efficient large fragment deletion in plants:double pairs of sgRNAs are better than dual sgRNAs显示文摘Dear Editor,Clustered Regularly Interspaced Short Palindromic Repeats(CRISPR)is a powerful and versatile gene editing system that has been extensively utilized in various animals and plants,which holds enormous potential and value for scientific research and breeding.However,single-targeted CRISPR can only induce a few base deletions,insertions,or substitution.Ideally,thesemutations result in premature termination of the protein encoded by the target gene,leading to a loss of function[1]. | Guoning Zhu Lingling Zhang Liqun Ma Qing Liu Kejian Wang Jinyan Li Guiqin Qu Benzhong Zhu Daqi Fu Yunbo Luo Hongliang Zhu | 2023 | Horticulture Research2023，10，10： | 1 |
| 13 | 显示文摘 | Lin Bixia Fu Zhuxi Jia Yunbo | 2001 | Appl Phys Lett2001，79，7： | 1 |
| 14 | Shen-Qi-Wan Protects the Renal Peritubular Capillary Injury from Adenine-mediated Damage by Upregulating Aquaporin 1显示文摘Background:Shen-Qi-Wan(SQW),a commonly used prescription against chronic kidney disease(CKD)in Tradi-tional Chinese Medicine(TCM),has a nephroprotective action in adenine-induced kidney injury.However,the mechanism of SQW in renal injury remains unclear.Objective:This study was undertaken to measure the effect of SQW on peritubular capillary injury both in vivo and in vitro assays.Methods:The effect of SQW on the peritubular capillary injury was evaluated according to measuring hypothalamic-pituitary-adrenal(HPA)function,inflammatory cytokines,VEGF(vascular endothelial growth fac-tor),CD34(Cluster of differentiation 34),and AQP1(Aquaporin 1)levels in the kidney,cell migration,and lumen forming capacity.Results:SQW supplementation could ameliorate dysfunction of the HPA and renal function loss induced by adenine.SQW also significantly inhibited the inflammatory cytokines including MCP-1(monocyte chemotactic protein-1)and VCAM-1(vascular cell adhesion molecule-1)level.Alternatively,SQW administration showed an ameliorating effect from the toxicity and alleviated the injury of capillaries around renal tubules instigated by adenine through increasing AQP1 mRNA and protein level.SQW medicated the serum enhanced the migration and lumen formation ability of HMEC-1 cells,and significantly increased AQP1 protein level.Moreover,AQP1 knockdown efficiently inhibited the migration and lumen formation ability in HMEC-1 cells,and weakened the effect of SQW medicated serum.Conclusion:These results suggested that SQW attenuated peritubular capillary injury in adenine-induced CKD model rats through boosting angiogenesis in endothelial cell,and AQP1 may be a potential target of SQW for treating the renal injury. | Yuting Bao Yehui Zhang Yuanxiao Yang Xueming Chen Luning Lin Yunbo Fu Liting Ji Changyu Li | 2022 | Clinical Complementary Medicine and Pharmacology2022，2，1： | 1 |
| 15 | Green luminescent center in undoped zinc oxide films deposited on silicon substrates显示文摘 | LIN Bixia FU Zhuxi JIA Yunbo | 2001 | Appl Phys Lett2001，79，7： | 1 |
| 16 | 显示文摘 | Lin Bixia Fu Zhuxi Jia Yunbo | 2001 | Appl Phys Lett2001，79，： | 1 |
| 17 | Green luminescent center in undoped zinc oxide films deposited on sili- con substrates 显示文摘 | Lin Bixia Fu Zhuxi Jia Yunbo | 2001 | Appl Phys Lett2001，79，7： | 1 |
| 18 | Robust photo-assisted removal of NO at room temperature:Experimental and density functional theory calculation with optical carrier显示文摘Photo-assisted SCR(PSCR) offers a potential solution for removal of NO at room temperature. MnTiO_(x)as PSCR catalyst exhibits superior performance with NO removal of 100% at the room temperature. Electron paramagnetic resonance(EPR) analysis revealed the presence of numerous oxygen vacancies on MnTiO_(x). Optical carrier density functional theory(DFT) calculations showed that the threedimensional orbital hybridization of Mn and Ti is significantly enhanced under light irradiation. The MnTiO_(x)catalyst exhibited excellent electron–hole separation ability, which can adsorbe NH_(3)and dissociate to form NH_(2)fragments and H atoms. In-situ diffuse reflectance infrared fourier-transform spectroscopy(DRIFTS) indicated that the optical carrier enhanced NH_(3)adsorption on MnTiO_(x), which makes it possess excellent PSCR activity. This work provided an additional strategy to NO removal with PSCR catalysts and showed potential for use in photocatalysis. | Yanqin Li Junqi Tian Zhisong Liu Zhongqi Liu Dong Dong Fu Wang Wei Wang Minmin Liu Jianming Dan Yongsheng Li Feng Yu Bin Dai Yunbo Yu | 2023 | Green Energy & Environment2023，8，4： | 0 |
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