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| 1 | Effects of dietary Clostridium butyricum supplementation on growth performance,intestinal development,and immune response of weaned piglets challenged with lipopolysaccharide显示文摘Background: Weanling pigs, with immature immune system and physiological function, usually experience postweaning diarrhea. This study determined the effects of dietary Clostridium butyricum supplementation on growth performance, diarrhea, and immunity of weaned pigs challenged with lipopolysaccharide(LPS).Methods: In Experiment(Exp.) 1,144 weaned piglets were weaned at 21 d and randomly assigned to six groups,with six replicates per group and four pigs per replicate, receiving a control diet(CON) or diet supplemented with antibiotics(AB) or C. butyricum(CB)(0.1%, 0.2%, 0.4%, or 0.8%), respectively. All diets in Exp. 1 were a highly digestible basal diet, with 3,000 mg/kg zinc oxide supplied in the first 2 wk only. In Exp. 2, 180 piglets were weaned at 21 d and randomly assigned to five groups, with six replicates per group and six pigs per replicate, receiving CON, AB, or CB(0.2%, 0.4%, or 0.6%) diets. The digestibility of diets was lower than those in Exp. 1, and did not include zinc oxide. At 36 d of Exp. 2, 12 piglets were selected from each of the CON and 0.4% CB groups, six piglets were intraperitoneally injected with LPS(50 μg/kg body weight) and the other six piglets with normal saline;animals were killed at 4 h after injection to collect blood, intestine, and digesta samples for biochemical analysis.Results: In Exp. 1, CB and AB diets had no effect on growth performance of piglets. In Exp. 2, 0.4% CB decreased feed-gain ratio(P < 0.1), diarrhea score(P < 0.05), and increased duodenal, jejunal, and ileal villus height and jejunal villus height/crypt depth(P < 0.05). The 0.4% CB decreased the plasma tumor necrosis factor(TNF) α(P < 0.05) but increased ileal mucosa IL-10 and TLR2 mRNA expression(P < 0.05). Furthermore, 0.4% CB altered the microbial profile, with Bacillus and Ruminococcaceae UGG-003 at genus level and Lactobacillus casei and Parasutterella secunda at species level were higher than CON in colonic content(P < 0.05).Conclusions: Dietary C. butyricum supplementation had positive effects on growth of weaned piglets with less digestible diets. There was a tendency to reduce the feed-gain ratio, which could reduce feed costs in pig production. Moreover, C. butyricum decreased post-weaning diarrhea by improving the intestinal morphology,intestinal microflora profile, and immune function. | Ling Chen Shuang Li Jie Zheng Wentao Li Xuemei Jiang Xilun Zhao Jian Li Lianqiang Che Yan Lin Shengyu Xu Bin Feng Zhengfeng Fang De Wu | 2018 | Journal of Animal Science and Biotechnology2018，9，4： | 26 |
| 2 | The Epitope Study on the SARS-CoV Nucleocapsid Protein显示文摘The nucleocapsid protein (N protein) has been found to be an antigenic protein in a number of coronaviruses. Whether the N protein in severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is antigenic remains to be elucidated. Using Western blot and Enzyme-linked Immunosorbent Assay (ELISA),the recombinant N proteins and the synthesized peptides derived from the N protein were screened in sera from SARS patients. All patient sera in this study displayed strong positive immunoreactivities against the recombinant N proteins,whereas normal sera gave negative immunoresponses to these proteins, indicating that the N protein of SARS-CoV is an antigenic protein. Furthermore, the epitope sites in the N protein were determined by competition experiments, in which the recombinant proteins or the synthesized peptides competed against the SARS-CoV proteins to bind to the antibodies raised in SARS sera. One epitope site located at the C-terminus was confirmed as the most antigenic region in this protein. A detailed screening of peptide with ELISA demonstrated that the amino sequence from Codons 371 to 407 was the epitope site at the C-terminus of the N protein. Understanding of the epitope sites could be very significant for developing an effective diagnostic approach to SARS. | Shuting Li, Liang Lin, Hao Wang, Jianning Yin, Yan Ren, Zhe Zhao, Jie Wen, Cuiqi Zhou, Xumin Zhang, Xiaolei Li, Jingqiang Wang, Zhengfeng Zhou, Jinxiu Liu, Jianmin Shao, Tingting Lei, Jianqiu Fang, Ningzhi Xu, and Siqi LiuBeijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China & Beijing Proteomics Institute, Beijing 101300, China | 2003 | Genomics, Proteomics & Bioinformatics2003，1，3： | 9 |
| 3 | Effects of dietary supplementation with essential oils and protease on growth performance,antioxidation,inflammation and intestinal function of weaned pigs显示文摘This experiment evaluated the impacts of essential oils(EO)and protease(PRO),independently or in combination,on growth performance,antioxidation,inflammation and intestinal function of weaned pigs.One hundred and sixty weaned pigs(21 d of age,BW of 6.74±0.20 kg)were randomly divided into 4 treatments with 8 replicate pens of 5 pigs per pen.Dietary treatments included the following:1)control diet(CON),2)CON with 300 mg/kg essential oils(EO),3)CON with 500 mg/kg protease(PRO),4)CON with 300 mg/kg essential oil and 500 mg/kg protease(EO + PRO).On d 8,one pig from each penwas selected for sampling.The remaining pigs were fed for an additional week and growth performance was monitored during this period.Dietary treatments had no marked effects(P>0.05)on the growth performance of pigs.However,pigs receiving EO diet had higher(P<0.05)serumglutathione peroxidase(GSH-Px)activity,and tended to decrease(P=0.063)serum concentration of tumor necrosis factor-a(TNF-a).In addition,pigs receiving EO diet had higher(P<0.05)abundances of phylum Actinobacteria,and genera Bifidobacterium,and lower(P<0.05)phylum Bacteroidetes and genera Alloprevotella in colonic digesta.Pigs receiving PRO diet decreased(P<0.05)the serum concentration of malondialdehyde(MDA)and diamine oxidase activity,increased(P<0.05)the villus height and the ratio of villus height to crypt depth in duodenum,increased sucrase activity in jejunal mucosa,and also increased the abundance of phylum Actinobacteria in colonic digesta.Furthermore,the synergistic effects of EO and PROwas observed(P<0.05)for pigs with decreasing serum TNF-a concentration and increasing serum GSH-Px activity.Collectively,the results indicated that dietary supplementation of EO and PRO had no significant effects on growth performance of weaned pigs.EO diet appeared to improve antioxidant activity and intestinal microbiota,while PRO diet improved intestinal morphology and digestive enzyme activity,and there was a synergistic effect of EO and PRO on reducing inflammatory parameters in weaned pigs. | Xie Peng Qiang Zhou Cheng Wu Jian Zhao Quan Tan Ying He Liang Hu Zhengfeng Fang Yan Lin Shengyu Xu Bin Feng Jian Li Yong Zhuo Chris Van Ginneken Xuemei Jiang De Wu Lianqiang Che | 2022 | Animal Nutrition2022，，2： | 8 |
| 4 | Establishment and Characterization of a Cell Based Artificial Antigen-Presenting Cell for Expansion and Activation of CD8^+ T Cells Ex Vivo显示文摘Artificial antigen-presenting cells are expected to stimulate the expansion and acquisition of optimal therapeutic features of T cells before infusion. Here CD32 that binds to a crystallizable fragment of IgG monoclonal antibody was genetically expressed on human K562 leukemia cells to provide a ligand for T-cell receptor. CD86 and 4-1BBL,which are ligands of co-stimulating receptors of CD28 and 4-1BB,respectively,were also expressed on K562 cells. Then we accomplished the artificial antigen-presenting cells by coupling K32/CD86/4-1BBL cell with OKT3 monoclonal antibody against CD3,named K32/CD86/4-1BBL/OKT3 cells. These artificial modified cells had the abilities of inducing CD8+ T cell activation,promoting CD8+ T cell proliferation,division,and long-term growth,inhibiting CD8+ T cell apoptosis,and enhancing CD8+ T cell secretion of IFN-γ and perforin. Furthermore,antigen-specific cytotoxic T lymphocytes could be retained in the culture stimulated with K32/CD86/4-1BBL/OKT3 cells at least within 28 days. This approach was robust,simple,reproducible and economical for expansion and activation of CD8+ T cells and may have important therapeutic implications for adoptive immunotherapy. | Weijuan Gong Mingchun Ji Zhengfeng Cao Liheng Wang Yayun Qian Maozhi Hu Li Qian Xingyuan Pan | 2008 | Cellular & Molecular Immunology2008，5，1： | 5 |
| 5 | Sin1/mTORC2 regulate B cell growth and metabolism by activating mTORC1 and Myc显示文摘Proper control of B cell growth and metabolism is crucial for B-cell-mediated immunity,but the underlying molecular mechanisms remain incompletely understood.In this study,Sin1,a key component of mTOR complex 2(mTORC2),specifically regulates B cell growth and metabolism.Genetic ablation of Sin1 in B cells reduces the cell size at either the transitional stage or upon antigen stimulation and severely impairs metabolism.Sin1 deficiency also severely impairs B-cell proliferation,antibody responses,and anti-viral immunity.At the molecular level,Sin1 controls the expression and stability of the c-Myc protein and maintains the activity of mTORC1 through the Akt-dependent inactivation of GSK3 and TSC1/2,respectively.Therefore,our study reveals a novel and specific role for Sin1 in coordinating the activation of mTORC2 and mTORC1 to control B cell growth and metabolism. | Man Li Adam S.Lazorchak Xinxing Ouyang Huihui Zhang Hongzhi Liu Omotooke A.Arojo Lichong Yan Jingsi Jin Yuheng Han Guojun Qu Yuhong Fu Xiaocao Xu Xiaobo Liu Wenqian Zhang Zhengfeng Yang Chuan Ruan Qijun Wang Dou Liu Chuanxin Huang Lu Lu Shibo Jiang Fubin Li Bing Su | 2019 | Cellular & Molecular Immunology2019，16，9： | 4 |
| 6 | Inclusion of purified dietary fiber during gestation improved the reproductive performance of sows显示文摘Background: This study aimed to investigate the impacts of guar gum and cellulose as the source of dietary fiber during gestation on the reproductive performance of sows.Methods: A total of 210 sows(parities 3–6) were randomly allocated into six diets(n = 35) throughout gestation to feed graded levels of dietary fiber(DF), including a corn-soybean meal-based control diet with no wheat bran inclusion(CON, 12.5% DF), a wheat bran-rich diet(DF1, 17.4% DF), and another 4 diets(DF2, 17.7% DF;DF3, 18.1%DF;DF4, 18.4% DF;DF5, 18.8% DF) in which wheat bran were equally substituted by 1%, 2%, 3% and 4% purified FIBER MIX(guar gum and cellulose, 1:4). All sows received similar DE and other nutrients throughout gestation.Results: DF treatment during gestation resulted in normal fecal score(1 to 5 with 1 = dry and 5 = watery) in sows compared with those received the CON diet(P < 0.05). The number of total born piglets had a tendency to be affected by dietary treatment(P = 0.07), and correlation analysis revealed a linear response of total born to dietary fiber levels during gestation(P < 0.01). Sows received the DF2, DF3, and DF5 diets during gestation had a greater ADFI during lactation compared with those in the CON group(P < 0.05) without affecting the daily body weight gain of suckling piglets. Gut microbiota compositions were dramatically changed by the gestation stage and some of those were changed by DF inclusion. Fecal acetate, propionate, and butyrate of sows were markedly increased in late gestation, and butyrate contents in feces of gestating sows were significantly affected by DF levels(P < 0.01).Serum concentrations of pro-inflammatory TNF-α were decreased and anti-inflammatory IL-10 was increased on day30 of gestation by DF levels(P < 0.05).Conclusions: In summary, increasing dietary fiber levels by guar gum and cellulose during gestation improved the reproductive performance of sows, which might be related to changes in immunity and gut microbiota of sows. | Yong Zhuo Bo Feng Yuedong Xuan Lianqiang Che Zhengfeng Fang Yan Lin Shengyu Xu Jian Li Bing Feng De Wu | 2020 | Journal of Animal Science and Biotechnology2020，11，4： | 4 |
| 7 | Circulating Tumor Cells in Hepatocellular Carcinoma: Detection Techniques, Clinical Implications, and Future Perspectives显示文摘 | Yu Zhang Jun Li Lu Cao Wen Xu Zhengfeng Yin | 2012 | Seminars in Oncology2012，，4： | 4 |
| 8 | Theoretical and simulation research of hydrodynamic instabilities in inertial-confinement fusion implosions显示文摘Inertial fusion energy(IFE)has been considered a promising,nearly inexhaustible source of sustainable carbon-free power for the world's energy future.It has long been recognized that the control of hydrodynamic instabilities is of critical importance for ignition and high-gain in the inertial-confinement fusion(ICF)hot-spot ignition scheme.In this mini-review,we summarize the progress of theoretical and simulation research of hydrodynamic instabilities in the ICF central hot-spot implosion in our group over the past decade.In order to obtain sufficient understanding of the growth of hydrodynamic instabilities in ICF,we first decompose the problem into different stages according to the implosion physics processes.The decomposed essential physics processes that are associated with ICF implosions,such as Rayleigh-Taylor instability(RTI),Richtmyer-Meshkov instability(RMI),Kelvin-Helmholtz instability(KHI),convergent geometry effects,as well as perturbation feed-through are reviewed.Analytical models in planar,cylindrical,and spherical geometries have been established to study different physical aspects,including density-gradient,interface-coupling,geometry,and convergent effects.The influence of ablation in the presence of preheating on the RTI has been extensively studied by numerical simulations.The KHI considering the ablation effect has been discussed in detail for the first time.A series of single-mode ablative RTI experiments has been performed on the Shenguang-Ⅱlaser facility.The theoretical and simulation research provides us the physical insights of linear and weakly nonlinear growths,and nonlinear evolutions of the hydrodynamic instabilities in ICF implosions,which has directly supported the research of ICF ignition target design.The ICF hot-spot ignition implosion design that uses several controlling features,based on our current understanding of hydrodynamic instabilities,to address shell implosion stability,has been briefly described,several of which are novel. | LiFeng Wang WenHua Ye XianTu He JunFeng Wu ZhengFeng Fan Chuang Xue HongYu Guo WenYong Miao YongTeng Yuan JiaQin Dong Guo Jia Jing Zhang YingJun Li Jie Liu Min Wang YongKun Ding WeiYan Zhang | 2017 | Science China(Physics,Mechanics & Astronomy)2017，60，5： | 4 |
| 9 | Dietary energy intake affects fetal survival and development during early and middle pregnancy in Large White and Meishan gilts显示文摘This experiment was designed to determine the effects of variations in dietary energy intake on reproductive performance and gene expression of luteal and endometrium tissues in Large White(LW)and Meishan(MS) gilts during early and middle pregnancy. After insemination, 32 LW gilts were assigned to high and low(HE_Land LE_L, 14.23 and 12.56 MJ DE/kg, respectively) diet treatment groups,while 32 MS gilts were allocated to HE_M and LE_M(12.56 and 10.88 MJ DE/kg) groups. Gilts were slaughtered on days 35, 55 and 90 of gestation. The fetal survival and luteal progesterone(P_4) concentration in the HE_Lgroup were higher on day 35 but lower on day 90 of gestation compared with the LE_L group(P < 0.05) for LW gilts. However, fetal survival and luteal P_4 concentration on day 35 of gestation were greater(P < 0.05) in the LE_M group than in the HE_M group for MS gilts, but no significant difference in mid-gestation was showed. The fetal weights of both breeds were higher for the high energy diets compared with the respective control group on day 90 of gestation(P < 0.05). In addition,the m RNA levels of P_4 synthesis-related proteins had correlated with luteal P_4 concentration in both breeds. Further, endometrial levels of uteroferrin(ACP5), retinol-binding protein 4(RBP_4) and secreted phosphoprotein 1(SPP1) m RNA were upregulated in the HE_Lgroup on day 35 of gestation but ACP5 and SPP1 were downregulated on day 55 of gestation compared with the LE_Lgroup(P < 0.05) for LW gilts. In MS gilts, diet only affected the expression of SPP1(P < 0.05). Our results revealed the differential sensitivity of LW and MS breeds to variations in dietary energy intake. For LW gilts, the HE_Lgroup improved fetal survival on day 35 but a sustained high energy diet decreased fetal survival on day 90 of gestation. The differences in dietary energy intake did not influence fetal survival on day 90 of gestation but the higher energy diet did increase fetal weight in the MS breed compared with the lower energy intake diet. These results may be due to differential luteal secretion activity and endometrium gene expression in these two breeds. | Long Che Zhenguo Yang Mengmeng Xu Ziyun Zhang Peilin Liu Shengyu Xu Lianqiang Che Yan Lin Zhengfeng Fang Bin Feng Jian Li De Wu | 2015 | Animal Nutrition2015，，3： | 4 |
| 10 | System construction of a four-side primary permanent-magnet linear motor drive mechanical press显示文摘A primary permanent-magnet linear motor (PPMLM) has a robust secondary structure and high force density and is appropriate for direct-drive mechanical press. The structure of a four-side PPMLM drive press is presented based on our previous research. The entire press control system is constructed to realize various flexible forming processes. The control system scheme is determined in accordance with the mathematical model of PPMLM, and active disturbance rejection control is implemented in the servo controller. Field-circuit coupling simulation is applied to estimate the system’s performance. Then, a press prototype with 6 kN nominal force is fabricated, and the hardware platform of the control system is constructed for experimental study. Punch strokes with 0.06 m displacement are implemented at trapezoidal speeds of 0.1 and 0.2 m/s;the dynamic position tracking errors are less than 0.45 and 0.82 mm, respectively. Afterward, continuous reciprocating strokes are performed, and the positioning errors at the bottom dead center are less than 0.015 mm. Complex pulse trajectories are also achieved. The proposed PPMLM drive press exhibits a fast dynamic response and favorable tracking precision and is suitable for various forming processes. | Jintao LIANG Zhengfeng MING Peida LI | 2020 | Frontiers of Mechanical Engineering2020，15，4： | 3 |
| 11 | Novel ACTG1 mutation causing autosomal dominant non-syndromic hearing impairment in a Chinese family显示文摘The γ-actin (ACTG1) gene is a cytoplasmic nonmuscle actin gene, which encodes a major cytoskeletal protein in the sensory hair cells of the cochlea. Mutations in ACTG1 were found to cause autosomal dominant, progressive, sensorineural hearing loss linked to the DFNA 20/26 locus on chromosome 17q25.3 in European and American families, respectively. In this study, a novel missense mutation (c.364A>G; p.I122V) co-segregated with the affected individuals in the family and did not exist in the unaffected family members and 150 unrelated normal controls. The alteration of residue Ile122 was predicted to damage its interaction with actin-binding proteins, which may cause disruption of hair cell organization and function. These findings strongly suggested that the I122V mutation in ACTG1 caused autosomal dominant non-syndromic hearing impairment in a Chinese family and expanded the spectrum of ACTG1 mutations causing hearing loss. | Ping Liu Hu Li Xiang Ren Haiyan Mao Qihui Zhu Zhengfeng Zhu Rong Yang Wenlin Yuan Jingyu Liu Qing Wang Mugen Liu | 2008 | Journal of Genetics and Genomics2008，35，9： | 3 |
| 12 | A NEW CONJUGATE GRADIENT METHOD AND ITS GLOBAL CONVERGENCE PROPERTIES显示文摘This paper presents a new conjugate gradient method for unconstrained opti-mization. This method reduces to the Polak-Ribiere-Polyak method when line searches areexact. But their performances are differellt in the case of inexact line search. By a simpleexample, we show that the Wolf e conditions do not ensure that the present method and thePolak- Ribiere- Polyak method will pro duce descent direct i0ns even u nder t h e ass umpt ionthat the objective function is Strictly convex. This result contradicts the F0lk axiom thatthe Polak-Ribiere-Polyak with the Wolf e line search should find the minimizer of a strictlyconvex objective function. Finally, we show that there are two ways to improve the newmethod such that it is globally convergent. | LI Zhengfeng CHEN Jing DENG Naiyang(Division of Basic Sciences, China Agricultural University East Campus, Beijing 100083, China) | 1998 | Systems Science and Mathematical Sciences1998，11，1： | 2 |
| 13 | Transfer of β-hydroxy-β-methylbutyrate from sows to their offspring and its impact on muscle fiber type transformation and performance in pigs显示文摘Background: Previous studies suggested that supplementation of lactating sows with β-hydroxy-β-methylbutyrate(HMB) could improve the performance of weaning pigs, but there were little information in the muscle fiber type transformation of the offspring and the subsequent performance in pigs from weaning through finishing in response to maternal HMB consumption. The purpose of this study was to determine the effect of supplementing lactating sows with HMB on skeletal muscle fiber type transformation and growth of the offspring during d 28 and180 after birth. A total of 20 sows according to their body weight were divided into the control(CON, n = 10) or HMB groups(HMB, n = 10). Sows in the HMB group were supplemented with β-hydroxy-β-methylbutyrate calcium(HMB-Ca) 2 g/kg feed during d 1 to 27 of lactation. After weaning, 48 mixed sex piglets were blocked by sow treatment and fed standard diets for post-weaning, growing, finishing periods. Growth performance was recorded during d 28 to 180 after birth. Pigs were slaughtered on d 28(n = 6/treatment) and 180(n = 6/treatment) postnatal and the longissimus dorsi(LD) was collected, respectively.Results: The HMB-fed sows during lactation showed increased HMB concentration(P < 0.05) in milk and LD of weaning piglets(P < 0.05). In addition, offsprings in HMB group had a higher finishing BW and lean percentage than did pigs in CON group(P < 0.05), meanwhile, compared with pigs from sows fed the CON diet, pigs from sows fed HMB diet showed higher type Ⅱ muscle fiber cross-sectional area(CSA), elevated myosin heavy chain(MyHC) Ⅱb and Sox6 mRNA, and fast-MyHC protein levels in LD(P < 0.05).Conclusions: HMB supplemented to sow diets throughout lactation increases the levels of HMB in maternal milk and skeletal muscle of pigs during d 28 after birth and promotes subsequent performance of pigs between d 28 and 180 of age by enhancing glycolytic muscle fiber transformation. | Haifeng Wan Jiatao Zhu Caimei Wu Pan Zhou Yong Shen Yan Lin Shengyu Xu Lianqiang Che Bin Feng Jian Li Zhengfeng Fang De Wu | 2017 | Journal of Animal Science and Biotechnology2017，8，2： | 2 |
| 14 | Structure modeling and spatial epitope analysis for HA protein of the novel H1N1 influenza virus显示文摘In recent months,a novel influenza virus H1N1 broke out around the world.With bioinformatics technology,the 3D structure of HA protein was obtained,and the epitope residues were predicted with the method developed in our group for this novel flu virus.58 amino acids were identified as potential epitope residues,the majority of which clustered at the surface of the globular head of HA protein.Although it is located at the similar position,the epitope of HA protein for the novel H1N1 flu virus has obvious differences in the electrostatic potential compared to that of HA proteins from previous flu viruses. | WU Di XU TianLei SUN Jing DAI JianXin DING GuoHui HE YunGang ZHOU ZhengFeng XIONG Hui DONG Hui JIN WeiRong BIAN Chao JIN Li WANG HongYan WANG XiaoNing YANG Zhong ZHONG Yang WANG Hao CHE XiaoYan HUANG Zhong LAN Ke SUN Bing WU Fan YUAN ZhenAn ZHANG Xi ZHOU XiaoNong ZHOU JiaHai MA ZhiYong TONG GuangZhi GUO YaJun ZHAO GuoPing LI YiXue CAO ZhiWei | 2009 | Chinese Science Bulletin2009，54，13： | 2 |
| 15 | Biomechanical change of lumbar unilateral graded facetectomy and strategies of its microsurgical reconstruction:report of 23 cases显示文摘评估腰部的稳定性和单方的 facetectomy 的主要临床的结果的目的, transforaminal 腰部的 interbody 熔化(TLIF ) 和由 X 试管的单方的小花梗螺丝钉固定系统。方法五人的腰部的 cadaveric 功能的脊骨单位(FSU ) 被 0 获得并且分级 facetectomy, 1/4, 1/2, 3/4 和 4/4 在他们的左关节的过程上分别地被执行。这 5 个模型的稳定性在屈曲,延期,侧面的弯曲和轴的旋转被评估。在 biomechanical 研究的一个连续剧以后,到在我们的部门的 2006 年 3 月的从 2004 年 6 月的 23 个病人经历了单方的 facetectomy, transforaminal 腰部的 interbody 熔化(以后的腰部的 interbody 熔化) 和由 X 试管的单方的小花梗螺丝钉乐器学系统。在一般麻醉以后,与荧光检查并且用 X 试管系统的指南,单方的内视镜的 facetectomy 的过程,针的神经根解压缩, autologus 多孔的骨头移植,一个笼子倾斜的插入和单方的小花梗螺丝钉乐器学被执行。在那里的结果不是在屈曲,延期,在单方的分级的 facetectomy 以后的侧面的弯曲和腰部的运动范围的轴的旋转的重要差别。当时,左 / 权利的轴的方向的稳定性极大地被影响分级的 facetectomy 的范围超过 1/2。根据 Nakai 标准,为 23 个病人,临床的结果在 15 是优秀的(65.2%) ,在 6 好(26.1%) 并且在 2 的交易会(8.7%) 。熔化率在优秀、好的盒子中是 95.6% 。尽管骨头接枝的部分吸收在可能显示失败的熔化的 1 种情况中被观察,没有松开,仪器和没有临床的症状的代替发生了。当分级的 facetectomy 的范围超过 1/2 时,腰部的稳定性将显著地被影响的结论。单方的 facetectomy 的过程, transforaminal 腰部的 interbody 熔化和单方的小花梗螺丝钉固定是为 microsurgical 重建的可选的策略,不过,过程的指示应该小心地被限制。 | Zhou Yue Luo Gang Chu Tongwei Wang Jian Li Changqing Zheng Wenjie Zhang Zhengfeng Hao Yong Zhang Chao | 2008 | Journal of Medical Colleges of PLA(China)2008，23，2： | 2 |
| 16 | Mesoporous Au-TiO2 nanocomposites with enhanced photocatalytic activity显示文摘 | LI Hexing BIAN Zhengfeng ZHU Jian | 2007 | Journal of the American Chemical Society2007，129，15： | 1 |
| 17 | SSJD15011900000922显示文摘 | Huiying Liu Xiaofeng Zhang Jun Li Bin Sun Haihua Qian Zhengfeng Yin | 2015 | Journal of Cancer Research and Clinical Oncology2015，，2： | 1 |
| 18 | Tandem Molecular Self-Assembly Selectively Inhibits Lung Cancer Cells by Inducing Endoplasmic Reticulum Stress显示文摘The selective formation of nanomaterials in cancer cells and tumors holds great promise for cancer diagnostics and therapy.Until now,most strategies rely on a single trigger to control the formation of nanomaterials in situ.The combination of two or more triggers may provide for more sophisticated means of manipulation.In this study,we rationally designed a molecule(Comp.1)capable of responding to two enzymes,alkaline phosphatase(ALP),and reductase.Since the A549 lung cancer cell line showed elevated levels of extracellular ALP and intracellular reductase,we demonstrated that Comp.1 responded in a stepwise fashion to those two enzymes and displayed a tandem molecular self-assembly behavior.The selective formation of nanofibers in the mitochondria of the lung cancer cells led to the disruption of the mitochondrial membrane,resulting in an increased level of reactive oxygen species(ROS)and the release of cytochrome C(Cyt C).ROS can react with proteins,resulting in endoplasmic reticulum(ER)stress and the unfolded protein response(UPR).This severe ER stress led to disruption of the ER,formation of vacuoles,and ultimately,apoptosis of the A549 cells.Therefore,Comp.1 could selectively inhibit lung cancer cells in vitro and A549 xenograft tumors in vivo.Our study provides a novel strategy for the selective formation of nanomaterials in lung cancer cells,which is powerful and promising for the diagnosis and treatment of lung cancer. | Debin Zheng Yumiao Chen Sifan Ai Renshu Zhang Zhengfeng Gao Chunhui Liang Li Cao Yaoxia Chen Zhangyong Hong Yang Shi Ling Wang Xingyi Li Zhimou Yang | 2019 | Research2019，，1： | 1 |
| 19 | Clinical application of massively parallel sequencing‐based prenatal noninvasive fetal trisomy test for trisomies 21 and 18 in 11 105 pregnancies with mixed risk factors显示文摘 | Shan Dan Wei Wang Jinghui Ren Yali Li Hua Hu Zhengfeng Xu Tze Kin Lau Jianhong Xie Weihua Zhao Hefeng Huang Jiansheng Xie Luming Sun Xiaohong Zhang Weipeng Wang Shixiu Liao Rong Qiang Jiangxia Cao Qiufang Zhang Yulin Zhou Haiyan Zhu Mei Zhong Yi Guo Linhu | 2012 | Prenat Diagn2012，，13： | 1 |
| 20 | Fibers reinforced composite hydrogels with improved lubrication and load-bearing capacity显示文摘Hydrogels as one kind of soft materials with a typical three-dimensional(3D)hydrophilic network have been getting great attention in the field of biolubrication.However,traditional hydrogels commonly show poor tribology performance under high-load conditions because of their poor mechanical strength and toughness.Herein,pure chemical-crosslinking hydrogels mixed with different types of the micron-scale fibers can meet the requirements of strength and toughness for biolubrication materials,meanwhile the corresponding tribology performance improves significantly.In a typical case,three kinds of reinforcement matrix including needle-punched fibers,alginate fibers,and cottons are separately combined with Poly(n-vinyl pyrrolidone)-poly 2-hydroxyethyl methacrylate(PVP-PHEMA)hydrogels to prepare fibers reinforced composite hydrogels.The experimental results show that the mechanical properties of fibers reinforced composite hydrogels improve greatly comparable with pure PVP-PHEMA hydrogels.Among three kinds of fibers reinforced composite hydrogel,the as-prepared composite hydrogels reinforced with needle-punched fibers possess the best strength,modulus,and anti-tearing properties.Friction tests indicate that the fibers reinforced composite hydrogels demonstrate stable water-lubrication performance comparable with pure PVP-PHEMA hydrogels.Besides,the hydrogel-spunlace fiber samples show the best load-bearing and anti-wear capacities.The improved tribology performance of the composite hydrogels is highly related to mechanical property and the interaction between the fibers and hydrogel network.Finally,spunlace fibers reinforced hydrogel materials with high load-bearing and low friction properties are expected to be used as novel biomimetic lubrication materials. | Jiawei LI Luyao GAO Rongnian XU Shuanhong MA Zhengfeng MA Yanhua LIU Yang WU Libang FENG Meirong CAI Feng ZHOU | 2022 | Friction2022，10，1： | 1 |
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