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| 1 | A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and Dicot Plants显示文摘CRISPR/Cas9 染色体指向系统被用于许多种类。然而,为植物报导的 mostCRISPR/Cas9 系统能仅仅修改一个或一些指向地点。这里,我们报导一个柔韧的 CRISPR/Cas9 向量系统,利用植物 codon 优化了 Cas9 基因,为方便、高效率的复合染色体在 monocot 和 dicot 植物编辑。我们设计了基于 PCR 的过程很快产生多重 sgRNA 表达式盒子,它能在一个轮由金门结扎或吉布森汇编克隆被装配进二进制 CRISPR/Cas9 向量。与这个系统,我们与 85.4% 变化评估的一般水准在米饭编辑了 46 个目标地点,主要在 biallelic 和同型结合的地位。我们推想在米饭的大约 16% 同型结合的变化通过相应基于再结合的修理跟随的非相应的加入结束的机制被产生。我们也获得了一致 biallelic,在 Arabidopsis T 1 植物的异质接合、同型结合、妄想的变化。在米饭和 Arabidopsis 的指向的变化是可继承的。我们在 T 0 米饭和 T 1 由同时的指向的 Arabidopsis 植物多重(多达八) 一个基因家庭,在一条 biosynthetic 小径的多重基因,或在单个基因的多重地点的成员。这个系统为为基本研究和基因改进在植物学习多重基因和基因家庭的功能提供了一个万用的工具箱。 | Xingliang Ma Qunyu Zhang Qinlong Zhu Wei Liu Yan Chen Rong Qiu Bin Wang Zhongfang Yang Heying Li Yuru Lin Yongyao Xie Rongxin Shen Shuifu Chen Zhi Wang Yuanling Chen Jingxin Guo Letian Chen Xiucai Zhao Zhicheng Dong Yao-Guang Liu | 2015 | Molecular Plant2015，8，8： | 272 |
| 2 | The Reference Genome Sequence of Scutellaria baicalensis Provides Insights into the Evolution of Wogonin Biosynthesis显示文摘Scutellaria baicalensis Georgi is important in Chinese traditional medicine where preparations of dried roots,'Huang Qin,' are used for liver and lung complaints and as complementary cancer treatments. We report a high-quality reference genome sequence for S. baicalensis where 93% of the 408.14-Mb genome has been assembled into nine pseudochromosomes with a super-N50 of 33.2 Mb. Comparison of this sequence with those of closely related species in the order Lamiales, sesamum indicum and Salvia splendens,revealed that a specialized metabolic pathway for the synthesis of 4'-deoxyflavone bioactives evolved in the genus Scu-tellaria. We found that the gene encoding a specific cinnamate coenzyme A ligase likely obtained its newfunc- tion following recent mutations, and that four genes encoding enzymes in the 4'-deoxyflavone pathway are present as tandem repeats in the genome of S. baicalensis. Further analyses revealed that gene duplications, segmental duplication, gene amplification, and point mutations coupled to gene neo- and subfunctionaliza-tions were involved in the evolution of 4'-deoxyflavone synthesis in the genus Scutellaria. Our study not only provides significant insight into the evolution of specific flavone biosynthetic pathways in the mint family, Lamiaceae, but also will facilitate the development of tools for enhancing bioactive productivity by metabolic engineering in microbes or by molecular breeding in plants. The reference genome of S. baicalensis is also useful for improving the genome assemblies for other members of the mint family and offers an important foundation for decoding the synthetic pathways of bioactive compounds in medicinal plants. | Qing Zhao Jun Yang Meng-Ying Cui Jie Liu Yumin Fang Mengxiao Yan Wenqing Qiu Huiwen Shang Zhicheng Xu Reheman Yidiresi Jing-Ke Weng Tomas Pluskal Marielle Vigouroux Burkhard Steuernagel Yukun Wei Lei Yang Yonghong Hu Xiao-Ya Chen Cathie Martin | 2019 | Molecular Plant2019，12，7： | 23 |
| 3 | Species-dependent neuropathology in transgenic SOD' aigs显示文摘 | Huaqiang Yang Guohao Wang Haitao Sun Runzhe Shu Tao Liu Chuan-En Wang Zhaoming Liu Yu Zhao Bentian Zhao Zhen Ouyang Dongshan Yang Jiao Huang Yueling Zhou Shihua Li Xiaodan Jiang Zhicheng Xiao Xiao-Jiang Li Liangxue Lai | 2014 | Cell Research2014，24，4： | 13 |
| 4 | Differentiation of adipose-derived stem cells toward nucleus pulposuslike cells induced by hypoxia and a three-dimensional chitosan-alginate gel scaffold in vitro显示文摘 | Zhang Zhicheng Li Fang Tian Haiquan Guan Kai Zhao Guangmin Shan Jianlin Ren Dajiang | 2014 | Chinese Medical Journal2014，，2： | 10 |
| 5 | Pathological changes in the lungs and lymphatic organs of 12 COVID-19 autopsy cases显示文摘Systematic autopsy and comprehensive pathological analyses of COVID-19 decedents should provide insights into the disease characteristics and facilitate the development of novel therapeutics.In this study,we report the autopsy findings from the lungs and lymphatic organs of 12 COVID-19 decedents—findings that evaluated histopathological changes;immune cell signature and inflammatory factor expression in the lungs,spleen and lymph nodes.Here we show that the major pulmonary alterations included diffuse alveolar damage,interstitial fibrosis and exudative inflammation featured with extensive serous and fibrin exudates,macrophage infiltration and abundant production of inflammatory factors(IL-6,IP-10,TNFo?and IL-I f).The spleen and hilar lymph nodes contained lesions with tissue structure disruption and immune cell dysregulation,including lymphopenia and macrophage accumulation.These findings provide pathological evidence that links injuries of the lungs and lymphatic organs with the fatal systematic respiratory and immune malfunction in critically ill COVID-19 patients. | Qian Liu Yu Shi Jun Cai Yaqi Duan Rongshuai Wang Hongyan Zhang Qiurong Ruan Jiansha Li Lei Zhao Yifang Ping Rong Chen Liang Ren Xiaochun Fei Heng Zhang Rui Tang Xi Wang Tao Luo Xindong Liu Xuequan Huang Zhenhua Liu Qilin Ao Yong Ren Jing Xiong Zhicheng He Haibo Wu Wenjuan Fu Pengnan Zhao Xinwei Chen Guoqiang Qu Yunyun Wang Xi Wang Jia Liu Dongfang Xiang Sanpeng Xu Xiaowei Zhou Qingrui Li Jinghong Ma Heng Li Jie Zhang Sizhe Huang Xiaohong Yao Yiwu Zhou Chaofu Wang Dingyu Zhang Guoping Wang Liang Liu Xiu-Wu Bian | 2020 | National Science Review2020，7，12： | 6 |
| 6 | Classification of Ferrallitic Soils in Chinese Soil Taxonomy显示文摘The development of the classification of ferrallitic soils in China is reviewed and the classification ofFerralisols and Ferrisols in Chinese Soil Taxonomy is introduced in order to discuss the correlation betweenthe ferrallitic soil classification in the Chinese Soil Taxonomy and those of the other soil classification systems.In the former soil classification systems of China, the ferrallitic soils were classified into the soil groups ofLatosols, Latosolic red soils, Red soils, Yellow soils and Dry red soils, according to the combination of soilforming conditions, soil-forming processes, soil features and soil properties. In the Chinese Soil Taxonomy,most of ferrallitic soils are classified into the soil orders of Ferralisols and Ferrisols based on the diagnostichorizons and/or diagnostic characteristics with quantitatively defined properties. Ferralisols are the soilsthat have ferralic horizon, and they are merely subdivided into one suborder and two soil groups. Ferrisolsare the soils that have LAC-ferric horizon but do not have ferralic horizon, and they are subdivided intothree suborders and eleven soil groups. Ferralisols may correspond to part of Latosols and Latosolic red soils.Ferrisols may either correspond to part of Red soils, Yellow soils and Dry red soils, or correspond to part ofLatosols and Latosolic red soils. | GONG ZITONG CHEN ZHICHENG ZHAO WENJUN and SHI HUA(Institute of Soil Science, the Chinese Academy of Sciences, P.O. Box 821, Naroing 210008 China) | 2000 | Pedosphere2000，10，2： | 5 |
| 7 | Synthesis of MoX2 (X =Se or S) monolayers with high-concentration 1T'phase on 4H/fcc-Au nanorods for hydrogen evolution显示文摘Controlled synthesis of transition metal dichalcogenide (TMD) monolayers with unusual crystal phases has attracted increasing attention due to their promising applications in electrocatalysis.However,the facile and large-scale preparation of TMD monolayers with high-concentration unusual crystal phase still remains a challenge.Herein,we report the synthesis of MoX2 (X =Se or S) monolayers with high-concentration semimetallic 1T'phase by using the 4H/face-centered cubic (fcc)-Au nanorod as template to form the 4H/fcc-Au@MoX2 nanocomposite.The concentrations of 1T'phase in the prepared MoSe2 and MoS2 monolayers are up to 86% and 81%,respectively.As a proof-of-concept application,the obtained Au@MoS2 nanocomposite is used for the electrocatalytic hydrogen evolution reaction (HER) in acid medium,exhibiting excellent performance with a low overpotential of 178 mV at the current density of 10 mNcm^2,a small Tafel slope of 43.3 mV/dec,and excellent HER stability.This work paves a way for direct synthesis of TMD monolayers with high-concentration of unusual crystal phase for the electrocatalytic application. | Zhengqing Liu Xiao Zhang Yue Gong Qipeng Lu Zhicheng Zhang Hongfei Cheng Qinglang Ma Junze Chen Meiting Zhao Bo Chen Ye Chen Xue-Jun Wu Pengfei Yin Lin Gu Yaping Du Hua Zhang | 2019 | Nano Research2019，12，6： | 3 |
| 8 | A Novel Roll Compensation Method for Two-Axis Transportable Satellite Antennas显示文摘Two-axis transportable satellite antennas(TATSAs) have been widely adopted owing to its simple structure and low cost. However, by searching in a wide range, it will take a very long searching time. Under extreme conditions, it will even fail to work. In this paper, we propose a novel roll compensation(RC) method for the low-cost TATSAs to achieve faster tracking even if when the antenna has no azimuth sensor. By analyzing the influence of roll axis on the system performance, details of the compensation method are derived. Simulation and measurement results indicate that the proposed RC method can effectively reduce the initial searching time for satellite communication. In addition, tracking along with the ellipse path with the RC method provides the highest tracking efficiency. | Laiding Zhao Jidong Xie Xiaodong Bai Zhicheng Qu | 2018 | China Communications2018，15，3： | 3 |
| 9 | Customized anterior segment photoacoustic imaging for ophthalmic burn evaluation in vivo显示文摘Photoacoustic imaging has many advantages in ophthalmic application including high-resolution,requirement of no exogenous contrast agent,and noninvasive acquisition of both morphologic and functional information.However,due to the limited depth of focus of the imaging method and large curvature of the eye,it remains a challenge to obtain high quality vascular image of entire anterior segment.Here,we proposed a new method to achieve high quality imaging of anterior segment.The new method applied a curvature imaging strategy based on only one time scanning,and hence is time efficient and more suitable for ophthalmic imaging compared to previously reported methods using similar strategy.A custom-built photoacoustic imaging system was adapted for ophthalmic application and a customized image processing method was developed to quantitatively analyze both morphologic and functional information in vasculature of the anterior segment.The results showed that the new method improved the image quality of anterior segment significantly compared to that of conventional high resolution photoacoustic imaging.More importantly,we applied the new method to study ophthalmic disease in an in vivo mouse model for the first time.The results verified the suitability and advantages of the new method for imaging the entire anterior segment and the numerous potentials of applying it in ophthalmic imaging in future. | Huangxuan Zhao Ke Li Fan Yang Wenhui Zhou Ningbo Chen Liang Song Chuansheng Zheng Zhicheng Liu Chengbo Liu | 2021 | Opto-Electronic Advances2021，4，6： | 2 |
| 10 | Hybrid Energy Sharing Mechanism for Integrated Energy Systems Based on the Stackelberg Game显示文摘Integrating the sharing economy and the power industry is of positive significance for the development of the energy market.With the energy market transforming from a traditional vertical structure to an interactive and competitive structure,users'roles need to change,along with supply and demand interacting more frequently.Thus,the traditional centralized optimization method for a single energy source can hardly reveal the complex multi-entity behavior of multi-energy coupling.Therefore,this paper establishes a distributed electrical-gas-thermal energy sharing mechanism centered on an energy hub that can converse energy,and build a more applicable integrated energy system body.First,the supply-demand interaction and the energy conversion process is constructed with reference to the operationa丨mode of’a sharing economy and the dual role of prosumers.A Stackelberg model is established with the integrated energy system operator as the leader and prosumers as the followers,to simultaneously optimize the profit of the leader in the upper level and the comfort of the fo!lowers,energy use and utility in the lower level.Furthermore,for protecting the participants’privacy,a distributed algorithm is used to find the optimal solution to equilibrate the model,and the existence and uniqueness of the solution is proved.Finally,a case study validates the effectiveness of the hybrid energy sharing mechanism and provides a reference for the integration of the energy sharing economy with the integrated energy system. | Qiao Peng Xiuli Wang Yi Kuang Yifei Wang Hongyang Zhao Zhicheng Wang Jianhu Lyu | 2021 | CSEE Journal of Power and Energy Systems2021，7，5： | 2 |
| 11 | 2021 Chinese consensus on the diagnosis and management of primary immune thrombocytopenia in pregnancy显示文摘Immune thrombocytopenia(ITP)is an acquired disease characterized by isolated thrombocytopenia,which is one of the most common causes of thrombocytopenia during pregnancy.Women with ITP who have severe thrombocytopenia are at an increased risk for life-threatening obstetric complications.Therefore,we established this consensus statement on the diagnosis and management of ITP during pregnancy(detailed information is available in the Supplementary File,http://gffzz16e312a87a7141a5hqcf560n9v0wv6wxf.ffgz.tsg.suse.edu.cn/A978). | Zhang Xiaohui Chen Fangping Chen Xiequn Cheng Yunfeng Fang Meiyun Feng Jianming Fu Haixia Gao Hong Han Yue He Aili Hou Ming Hu Yu Huang Ruibin Huang Wenrong Jing Zhicheng Kong Peiyan Liang Aibin Liang Meiying Liu Daihong Liu Junling Liu Lin Liu Xiaowei Ma Liangming Mei Heng Ni Heyu Niu Ting Peng Jun Qiao Jianlin Ren Jinhai Song Yongping Tang Liang V Tong Tong Wang Shaoyuan Wang Xin Wang Zhao Wei Hui Wu Depei Wu Guangsheng Xu Caigang Xu Xue Xu Yajing Yang Linhua Yang Renchi Yang Tonghua Yin Chenghong Yu Li Zhang Guangsen Zhang Lei Zhang Liansheng Zhang Xi Zhao Weili Zhao Yongqiang Zhou Daobin Zhou Hu Zhou Zeping Zhu Tienan Wang Jianliu Huang Xiaojun | 2022 | Chinese Medical Journal2022，，8： | 2 |
| 12 | Adaptive Fuzzy Sliding Mode Control for Uncertain Nonlinear Systems显示文摘 | Jing-gang Zhang Zhicheng Zhao and Jianchao Zeng | 2003 | IEEE Transactions on Proceedings of the Second International Conference on Machine Learning and Cybernetics2003，，： | 1 |
| 13 | Clinical characteristics of pulmonary hypertension in bronchiectasis显示文摘作为 bronchiectasis 的复杂并发症,肺的高血压(PH ) 与增加的死亡被联系。然而,血液动力学的特征和肺的动脉的高血压的功效(哼) 在有 bronchiectasis 和 PH 的病人的治疗仍然保持未知。有双边的 bronchiectasis 和并发的 PH 的病人在学习被包括。在基线并且在后续期间的耐心的特征,以及幸存,被分析。这观察研究与 51.5 年的吝啬的年龄在 36 个病人被进行(范围, 1774 年) 。走距离的 6 min 是 300.8 搠睯 吗?? | Lan Wang Sen Jiang Jingyun Shi Sugang Gong Qinhua Zhao Rong Jiang Ping Yuan Bigyan Pudasaini Jing He Zhicheng Jing Jinming Liu | 2016 | Frontiers of Medicine2016，10，3： | 1 |
| 14 | Backstepping sliding mode tracking control of quad-rotor under input saturation显示文摘Purpose–The quad-rotor is an under-actuation,strong coupled nonlinear system with parameters uncertainty,unmodeled disturbance and drive capability boundedness.The purpose of the paper is to design a flight control system to regulate the aircraft track the desired trajectory and keep the attitude angles stable on account of these issues.Design/methodology/approach–Considering the dynamics of a quad-rotor,the closed-loop flight control system is divided into two nested loops:the translational outer-loop and the attitude inner-loop.In the outer-loop,the translational controller,which exports the desired attitude angles to the inner-loop,is designed based on bounded control technique.In consideration of the influence of uncertain rotational inertia and external disturbance,the backstepping sliding mode approach with adaptive gains is used in the inner-loop.The switching control strategy based on the sign functions of sliding surface is introduced into the design procedure with respect to the input saturation.Findings–The validity of the proposed flight control system was verified through numerical simulation and prototype flight experiment in this paper.Furthermore,with relation to the flying,the motor speed is kept in the predetermined scope.Originality/value–This article introduces a new flight control system designed for a quad-rotor. | Xun Gong Yue Bai Zhicheng Hou Changjun Zhao Yantao Tian Qiang Sun | 2012 | International Journal of Intelligent Computing and Cybernetics2012，5，4： | 1 |
| 15 | Heterojunction interface editing in Co/NiCoP nanospheres by oxygen atoms decoration for synergistic accelerating hydrogen and oxygen evolution electrocatalysis显示文摘Controllable designing of well-defined heterojunction nanostructures provides an insightful strategy for accelerating the kinetics of the hydrogen and oxygen evolution reactions(HER/OER),but such task is still challenging.Herein,we proposed a protocol of heterojunction interface editing(HIE)strategy by oxygen atoms decoration for synergistic boosting electrocatalytic HER and OER performances.A novel Co/NiCoP nanospheres(NSs)heterojunction was synthesized by crystal seed template transformation method with Ni_(5)P_(4) microspheres as seeds.The effective oxygen atoms interface editing increased the oxidation state of Co atoms and prolonged the Co-P bond length of Co/NiCoP NSs heterojunction,thus the electron localization on P sites was enhanced,leading to the dramatically elevated HER and OER performances simultaneously.The as-constructed O-Co/NiCoP NSs show excellent electrocatalytic activity with 361 and 430 mV vs.reversible hydrogen electrode(RHE)to arrive high current density of 300 mA·cm^(-2)for HER and OER in 1 M KOH as well as good stability.The proposed HIE concept could provide a new perspective on the catalyst design for energy conversion systems. | Yan Lin Xiaojiao Cui Yilin Zhao Zhicheng Liu Guoxin Zhang Yuan Pan | 2023 | Nano Research2023，16，7： | 1 |
| 16 | The influence of pore structures and Lewis acid sites on selective hydrogenolysis of guaiacol to benzene over Ru/TS-1显示文摘Here,we report a Ru/TS-1 catalyst for selective hydrogenolysis of guaiacol to benzene in the aqueous phase at conditions of 240℃ and 0.2 MPa H2,achieving a benzene yield of 86% with a hydrogenolysis rate of 103.1 mmol g^(-1) h^(-1).It was found that Silicalite-1(MFI type) with suitable pore sizes supported Ru nanoparticles(NPs) favored for hydrogenolysis of guaiacol,whereas de-aluminated Ru/HBEA,Ru/HY and Ru/MWW(without acidic sites) accelerated the parallel reactions of hydrogenation of aromatics.In addition,Ru NPs located at the orifice of Silicalite-1 was proved to be more electron-deficient and active than Ru NPs on the outer surface,as evidenced by CO-IR characterization and activity tests on Ru/Silicalite-1(with and without templates).Moreover,Br?nsted acid sites(BAS) on Ru/MFI highly promoted the hydrogenation rates of aromatics,while Lewis acid sites(LAS) on Ru/TS-1 and Ru/MFI led to a linear increase of guaiacol hydrogenolysis rate to benzene,probably due to the enhanced absorbance capability of guaiacol and phenol on the LAS of MFI.Thus,pore structure properties of MFI coupled with abundant LAS(TS-1) as well as Ru NPs on the orifice of pores of TS-1 construct a promising catalyst for achieving efficient aromatic hydrocarbons from selective hydrogenolysis of lignin. | Yingying Xin Zhaoxia Zheng Zhicheng Luo Chengzhen Jiang Shaofeng Gao Zhihao Wang Chen Zhao | 2022 | Green Energy & Environment2022，7，5： | 1 |
| 17 | Dl-3-n-butylphthalide, a natural antioxidant, protects dopamine neurons in rotenone models for Parkinson’s disease显示文摘 | Nian Xiong Jinsha Huang Chunnuan Chen Ying Zhao Zhaowen Zhang Min Jia Zhentao Zhang Lingling Hou Hecheng Yang Xuebing Cao Zhihou Liang Yongxue Zhang Shenggang Sun Zhicheng Lin Tao Wang | 2012 | Neurobiology of Aging2012，，8： | 1 |
| 18 | Serum Helicobacter pylori CagA antibody may not be used as a tumor marker for diagnosing gastric cancer in east Asian countries显示文摘 | Zhicheng Zhao Yuhong Li Shengxin Liu Weihua Fu | 2014 | Tumor Biology2014，，12： | 1 |
| 19 | Wide-temperature range and high safety electrolytes for highvoltage Li-metal batteries显示文摘Along with the keeping growing demand for high-energy-density energy storage system,high-voltage Li-metal batteries(LMBs)have attracted many attentions.In view of many defects of the commercial electrolytes,such as flammability,limited operation temperature range,and severe Li dendrite growth,non-flammable phosphate-based localized highly concentrated electrolytes(LHCE)have been explored as one of the safe electrolytes for LMBs.But until now there is rare report on wide-temperature range LMBs using phosphate-based electrolytes.Here,we prepare a wide-temperature LHCE,which is composed of lithium difluoro(oxalato)borate(LiDFOB),triethyl phosphate(TEP),and 1,1,2,2-tetrafluoroethyl-2,2,3,3-tetrafluoropropyl ether(HFE),and explore the applicability in wide-temperature LMBs from−40 to 70℃.In the LHCE,both TEP and HFE are non-flammable,and Li^(+) is highly coordinated with TEP and DFOB^(−),which can effectively inhibit the TEP decomposition on anode,and facilitate the preferential reduction of DFOB^(−),thus obtain a robust solid electrolyte interphase(SEI)to suppress Li dendrite growth and side reactions.Therefore,this LHCE can not only endow Li/Cu and Li/Li cells with high Coulombic efficiency(CE)and long cycling lifespan,but also be applied to LiFePO_(4)(LFP)/Li and LiNi_(0.5)Co_(0.2)Mn_(0.3)O_(2)(NCM523)/Li LMBs.Most importantly,the NCM523/Li LMBs with LHCE can deliver stable cycling performance at 4.5 V high-voltage and high-temperature(70℃),as well as excellent low-temperature capacity retention even though both charging and discharging process were carried out at−40℃. | Anran Pan Zhicheng Wang Fengrui Zhang Lei Wang Jingjing Xu Jieyun Zheng Jianchen Hu Chenglong Zhao Xiaodong Wu | 2023 | Nano Research2023，16，6： | 1 |
| 20 | Ultra-thin metal-organic framework nanoribbons显示文摘Structure engineering of metal-organic frameworks(MOFs)at the nanometer scale is attracting increasing interest due to their unique properties and new functions that normally cannot be achieved in bulk MOF crystals.Here,we report the preparation of ultra-thin MOF nanoribbons(NRBs)by using metal-hydroxide nanostructures as the precursors.Importantly,this general method can be used to synthesize various kinds of ultra-thin MOF NRBs,such as MBDC(M=Co,Ni;BDC=1,4-benzenedicarboxylate),NiCoBDC,CoTCPP(TCPP=tetrakis(4-carboxyphenyl)porphyrin)and MIL-53(Al)NRBs.As a proof-of-concept application,the as-prepared ultra-thin Co BDC NRBs have been successfully used as a fluorescent sensing platform for DNA detection,which exhibited excellent sensitivity and selectivity.The present strategy might open an avenue to prepare MOF nanomaterials with new structures and unique properties for various promising applications. | Bingqing Wang Meiting Zhao Liuxiao Li Ying Huang Xiao Zhang Chong Guo Zhicheng Zhang Hongfei Cheng Wenxian Liu Jing Shang Jing Jin Xiaoming Sun Junfeng Liu Hua Zhang | 2020 | National Science Review2020，7，1： | 1 |
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