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| 1 | Analysis of DNA methylation variation in wheat genetic background after alien chromatin introduction based on methylation-sensitive amplification polymorphism显示文摘During the process of alien germplasm introduced into wheat genome by chromosome engineering, extensive genetic variations of genome structure and gene expression in recipient could be induced. In this study, we performed GISH (genome in situ hybridization) and AFLP (amplified fragment length po-lymorphism) on wheat-rye chromosome translocation lines and their parents to detect the identity in genomic structure of different translocation lines. The results showed that the genome primary struc-ture variations were not obviously detected in different translocation lines except the same 1RS chromosome translocation. Methylation sensitive amplification polymorphism (MSAP) analyses on genomic DNA showed that the ratios of fully-methylated sites were significantly increased in translo-cation lines (CN12, 20.15%; CN17, 20.91%; CN18, 22.42%), but the ratios of hemimethylated sites were significantly lowered (CN12, 21.41%; CN17, 23.43%; CN18, 22.42%), whereas 16.37% were fully-me-thylated and 25.44% were hemimethylated in case of their wheat parent. Twenty-nine classes of me-thylation patterns were identified in a comparative assay of cytosine methylation patterns between wheat-rye translocation lines and their wheat parent, including 13 hypermethylation patterns (33.74%), 9 demethylation patterns (22.76%) and 7 uncertain patterns (4.07%). In further sequence analysis, the alterations of methylation pattern affected both repetitive DNA sequences, such as retrotransposons and tandem repetitive sequences, and low-copy DNA. | ZHANG Yong LIU ZhaoHui LIU Cheng YANG ZuJun DENG KeJun PENG JinHua ZHOU JianPing LI GuangRong TANG ZongXiang REN ZhengLong | 2008 | Chinese Science Bulletin2008，53，1： | 8 |
| 2 | Zanthoxylum-specific whole genome duplication and recent activity of transposable elements in the highly repetitive paleotetraploid Z.bungeanum genome显示文摘Zanthoxylum bungeanum is an important spice and medicinal plant that is unique for its accumulation of abundant secondary metabolites,which create a characteristic aroma and tingling sensation in the mouth.Owing to the high proportion of repetitive sequences,high heterozygosity,and increased chromosome number of Z.bungeanum,the assembly of its chromosomal pseudomolecules is extremely challenging.Here,we present a genome sequence for Z.bungeanum,with a dramatically expanded size of 4.23 Gb,assembled into 68 chromosomes.This genome is approximately tenfold larger than that of its close relative Citrus sinensis.After the divergence of Zanthoxylum and Citrus,the lineage-specific whole-genome duplication event q-WGD approximately 26.8 million years ago(MYA)and the recent transposable element(TE)burst~6.41 MYA account for the substantial genome expansion in Z.bungeanum.The independent Zanthoxylum-specific WGD event was followed by numerous fusion/fission events that shaped the genomic architecture.Integrative genomic and transcriptomic analyses suggested that prominent speciesspecific gene family expansions and changes in gene expression have shaped the biosynthesis of sanshools,terpenoids,and anthocyanins,which contribute to the special flavor and appearance of Z.bungeanum.In summary,the reference genome provides a valuable model for studying the impact of WGDs with recent TE activity on gene gain and loss and genome reconstruction and provides resources to accelerate Zanthoxylum improvement. | Shijing Feng Zhenshan Liu Jian Cheng Zihe Li Lu Tian Min Liu Tuxi Yang Yulin Liu Yonghong Liu He Dai Zujun Yang Qing Zhang Gang Wang Jisen Zhang Huifeng Jiang Anzhi Wei | 2021 | Horticulture Research2021，8，1： | 5 |
| 3 | Comparative FISH and molecular identification of new stripe rust resistant wheat-Thinopyrum intermedium ssp. trichophorum introgression lines显示文摘Thinopyrum intermedium(2 n = 6 x = 42, JJJ^sJ^sStSt) has been hybridized extensively with common wheat and has proven to be a valuable germplasm source for improving disease resistance, quality attributes, and yield potential in wheat. We characterized new disease resistant wheat-Th. intermedium derivatives A1082 and A5-5 using sequential multi-color fluorescence in situ hybridization(mc-FISH), genomic in situ hybridization(GISH), PCRbased landmark unique gene(PLUG) and intron targeting(IT) markers. A1082 was identified as a wheat-Th. intermedium 3J disomic addition line, and A5-5 was a T4BS·5J^sL homozygous Robertsonian translocation line. Seventy-one and 106 pairs of primers amplified Th.intermedium-specific bands allowing chromosomes 3J and 5J^s to be tracked, respectively. A new oligonucleotide probe, Oligo-6 H-2-100, was developed for FISH labeling of the subterminal region of the long arm of chromosome 5J^s. Both lines were highly resistant to stripe rust pathogen races prevalent in Chinese field screening nurseries. A5-5 also displayed a significant increase in tiller number compared to its wheat parent. The new lines can be exploited as useful germplasms for wheat improvement. | Jianbo Li Qiheng Chen Peng Zhang Tao Lang Sami Hoxha Guangrong Li Zujun Yang | 2019 | The Crop Journal2019，7，6： | 2 |
| 4 | Adaptive microclimatic evolution of the dehydrin 6 gene in wild barley at “Evolution Canyon”, Israel显示文摘 | Zujun Yang Tao Zhang Guangrong Li Eviatar Nevo | 2011 | Genetica2011，，11： | 1 |
| 5 | Maternal genetic history of ancient Tibetans over the past 4000 years显示文摘The settlement of the Tibetan Plateau epitomizes human adaptation to a high-altitude environment that poses great challenges to human activity.Here,we reconstruct a 4000-year maternal genetic history of Tibetans using 128 ancient mitochondrial genome data from 37 sites in Tibet.The phylogeny of haplotypes M9a1a,M9a1b,D4g2,G2a’c,and D4i show that ancient Tibetans share the most recent common ancestor with ancient Middle and Upper Yellow River populations around the Early and Middle Holocene.In addition,the connections between Tibetans and Northeastern Asians vary over the past 4000 years,with a stronger matrilineal connection between the two during 4000 BPe3000 BP,and a weakened connection after 3000 BP,that are coincident with climate change,followed by a reinforced connection after the Tubo period(1400 BPe1100 BP).Besides,an over 4000-year matrilineal continuity is observed in some of the maternal lineages.We also find the maternal genetic structure of ancient Tibetans is correlated to the geography and interactions between ancient Tibetans and ancient Nepal and Pakistan populations.Overall,the maternal genetic history of Tibetans can be characterized as a long-term matrilineal continuity with frequent internal and external population interactions that are dynamically shaped by geography,climate changes,as well as historical events. | Ganyu Zhang Can Cui Shargan Wangdue Hongliang Lu Honghai Chen Lin Xi Wei He Haibing Yuan Tinley Tsring Zujun Chen Feng Yang Tashi Tsering Shuai Li Norbu Tashi Tsho Yang Yan Tong Xiaohong Wu Linhui Li Yuanhong He Peng Cao Qingyan Dai Feng Liu Xiaotian Feng Tianyi Wang Ruowei Yang Wanjing Ping Ming Zhang Xing Gao Yichen Liu Wenjun Wang Qiaomei Fu | 2023 | Journal of Genetics and Genomics2023，50，10： | 1 |
| 6 | Molecular cytogenetic characterization of a new wheat Secale africanum 2R a (2D) substitution line for resistance to stripe rust显示文摘 | MENGPING LEI GUANGRONG LI SUFEN ZHANG CHENG LIU ZUJUN YANG | 2011 | Journal of Genetics2011，，2： | 1 |
| 7 | Identification of conserved microRNAs and their target genes in tomato ( Lycopersicon esculentum )显示文摘 | Zujun Yin Chunhe Li Xiulan Han Fafu Shen | 2008 | Gene2008，，1： | 1 |
| 8 | Genome-wide profiling of miRNAs and other small non-coding RNAs in the Verticillium dahliae-inoculated cotton roots显示文摘 | Yin Zujun Li Yan Han Xiulan | 2012 | PLoS One2012，7，35： | 1 |
| 9 | A novel method for reconstruction in laparoscopic pancreaticoduodenectomy:an experience of 13 cases显示文摘Laparoscopic pancreaticoduodenectomy(LPD)is a challenging operation to general surgeon.Up to date,only about 135 cases have been reported,16 cases in China,119 cases outside China.The reconstruction of alimentary system is a key procedure to ensure success of the whole surgery.It is worth investigating the methods of reconstruc-tion in LPD.A retrospective study is made to investigate the methods of reconstruction in LPD.We analyze 13 cases of LPD performed in our center.Child’s or modified Child’s method was used to make the reconstruction in our practice.We tried three methods to make the anastomosis of pancre-aticojejunostomy,including end-to-end dunking binding pancreaticojejunostomy in two cases,end-to-end dunking pancreaticojejunostomy using interrupted suture in two cases,and duct-to-jejunal end-to-side embedding pancreaticojeju-nostomy in nine cases.The clinical data was collected and analyzed.Three of four patients,who underwent end-to-end pancreaticojejunostomy,had a little pancreatic leakage,especially in the first case.None of other nine patients,who underwent duct-to-jejunal end-to-side embedding pancreati-cojejunostomy,was detected to have pancreatic leakage,and the operating time of these nine cases was less than other four cases.Duct-to-jejunal end-to-side embedding pancreaticoje-junostomy is a safe and efficient method of reconstruction in LPD. | LU Bangyu HUANG Yubin CAI Xiaoyong HUANG Fei LI Jie LU Wenqi XU Jing LIU Zujun YAN Yihe LI Jianjun | 2007 | Frontiers of Medicine2007，1，4： | 1 |
| 10 | The Evaluation of Educational Literacy of Physics Students in Normal Universities Under China’s Professional Certification System显示文摘This article discusses the connotation of the evaluation of educational literacy of physics students in normal universities,provides the evaluation criteria for students’teaching ability,improves the evaluation composition of educational literacy of physics students in normal universities,and provides the score distribution of the 10 items in educational literacy,in hope to improve the training quality of university students. | Zujun Li Qihang Zhou Shiwen Ni Tian Liang Xingyao Han | 2022 | Journal of Contemporary Educational Research2022，6，1： | 0 |
| 11 | NK cell depletion promotes liver metastasis of lung cancer cells显示文摘Lung cancer is the malignant tumor with the highest morbidity and mortality in the world[1].Metastasis is the main cause of death in lung cancer patients[2].Cancer cells that are shed from the primary tumor tissue and enter the peripheral circulation are called circulating tumor cells(CTCs)[3].Among them,tumor cells that reach and colonize distant target organs are named disseminated tumor cells(DTCs)[4].Influenced by the microenvironment,immune surveillance,angiogenesis and other factors,DTCs have a weak ability to proliferate or even enter a dormant state[5].Therefore,reducing proliferative activity or maintaining the dormancy of DTCs has emerged as a novel strategy to prevent lung cancer metastasis. | Pan Yu Long Zhang Jianhui Tian Jiajun Liu Zujun Que Ge Li Yiyang Zhou | 2024 | Acta Biochimica et Biophysica Sinica2024，56，2： | 0 |
| 12 | China's Economic Growth:The“Two-Dimensional Driving Effect”of Data Factors显示文摘Data factors have become one of the five essential production factors,but their role in economic growth has always been ambiguous.Starting from AI technologies,this paper establishes an endogenous growth model of data factors affecting economic growth,constructs the generation path and value path of data factors,and estimates the value of new data factors at the provincial level in China from 1999 to 2018 accordingly.Based on theoretical analyses and empirical tests,it clarifes that data factors have a“two-dimensional driving effect”on China's economic growth,that is,data factors can drive growth both directly through its own economic growth effect and indirectly by promoting technological progress.Furthermore,this paper makes three extended discussions,aiming to make a trial study on the impacts of local government big data transaction platforms on data factors and their growth effects,discuss whether it is possible to reduce the uncertainties of local economic policy based on the nature of data factors,and make a preliminary survey of the output elasticity of data factors between 1999 and 2018. | Yan Yang Li Wang Yujia Li Zujun Liao | 2023 | China Finance and Economic Review2023，12，4： | 0 |
| 13 | Influence of CO_(2)/HCO_(3)^(-) on Microbial Communities in Two Karst Caves with High CO_(2)显示文摘There is limited knowledge about microbial communities and their ecological functions in karst caves with high CO_(2)concentrations.Here,we studied the microbial community compositions and functions in Shuiming Cave( 'SMC',CO_(2)concentration 3 303 ppm) and Xueyu Cave( 'XYC',CO_(2)concentration 8 753 ppm) using Illumina MiSeq high-throughput sequencing in combination with BIOLOG test.The results showed that Proteobacteria,Actinobacteria and Bacteroidetes were dominant phyla in these two caves,and Thaumarchaeota was the most abundant in the rock wall samples of SMC.The microbial diversity in the water samples decreased with increasing HCO_(3)^(-)concentration,and it was higher in XYC than that in SMC.The microbial community structures in the sediment and rock wall samples were quite different between the two caves.High concentrations of CO_(2)can reduce the microbial diversity on the rock walls in karst caves,probably through changing microbial preference for different types of carbon sources and decreasing the microbial utilization rate of carbon sources.These results expanded our understanding of microbial community and its response to environments in karst caves with high CO_(2). | Jiyu Chen Qiang Li Qiufang He Heinz C.Schroder Zujun Lu Daoxian Yuan | 2023 | Journal of Earth Science2023，34，1： | 0 |
| 14 | Jinfukang inhibits clustering and invasion of circulating lung tumor cells by regulating the EGFR signaling pathway显示文摘Lung cancer is the leading cause of cancer-related death in China and worldwide[1].Although the popularization and application of PET-CT have improved the detection rate of patients with early lung cancer,there is still a lack of effective drugs to prevent and treat lung cancer metastasis and prolong the survival of patients[2,3].Circulating tumor cells(CTCs),as seeds of tumor metastasis,play an important role in the metastasis of lung cancer. | Yun Yang Lihua Zhu Jiajun Liu Pan Yu Zujun Que Yan Li Hegen Li Jianhui Tian | 2023 | Acta Biochimica et Biophysica Sinica2023，55，11： | 0 |
| 15 | Theoretical analysis on GaAs sub-cell doping concentration for triple-junction solar cells irradiated by proton based on TCAD simulation显示文摘The degradation on the GaInP/GaAs/Ge triple-junction solar cells was irradiated by proton, and the solar cells with various GaAs sub-cell doping concentrations are modeled by the technology computer aided design(TCAD) simulation. The degradation results of related electrical parameters and external quantum efficiency(EQE) are studied. The degradation mechanism irradiated by proton is discussed. The short-circuit current, maximum power and conversion efficiency decrease with the increasing of GaAs sub-cell doping concentration. When the base doping concentration of GaAs sub-cell is 1×1016cm-3, the degradation of short-circuit current is less than that of other base doping concentrations. Furthermore, under proton irradiation, with the increase of doping concentration of GaAs sub-cell, the open-circuit voltage first increases and then decreases. Meanwhile, when the base doping concentration of GaAs sub-cell is 2×10^(17)cm^(-3), the degradation of open-circuit voltage is less than that of other base doping concentrations. The research will provide the basic theories and device simulation method for GaInP/GaAs/Ge triple-junction solar cells radiation damage evaluation study and radiation hardening, and can provide guidance for the production of triple-junction solar cells in orbit. | LI Junwei JIA Weimin SHI Chengying WANG Zujun LI Zhengcao | 2022 | Optoelectronics Letters2022，18，12： | 0 |
| 16 | Band-gap tunable(Ga_(x)In_(1−x))_(2)O_(3)layer grown by magnetron sputtering显示文摘Multicomponent oxide(Ga_(x)In_(1−x))_(2)O_(3)films are prepared on(0001)sapphire substrates to realize a tunable band-gap by magnetron sputtering technology followed by thermal annealing.The optical properties and band structure evolution over the whole range of compositions in ternary compounds(Ga_(x)In_(1−x))_(2)O_(3)are investigated in detail.The X-ray diffraction spectra clearly indicate that(Ga_(x)In_(1−x))_(2)O_(3)films with Ga content varying from 0.11 to 0.55 have both cubic and monoclinic structures,and that for films with Ga content higher than 0.74,only the monoclinic structure appears.The transmittance of all films is greater than 86%in the visible range with sharp absorption edges and clear fringes.In addition,a blue shift of ultraviolet absorption edges from 380 to 250 nm is noted with increasing Ga content,indicating increasing band-gap energy from 3.61 to 4.64 eV.The experimental results lay a foundation for the application of transparent conductive compound(Ga_(x)In_(1−x))_(2)O_(3)thin films in photoelectric and photovoltaic industry,especially in display,light-emitting diode,and solar cell applications. | Fabi ZHANG Jinyu SUN Haiou LI Juan ZHOU Rong WANG Tangyou SUN Tao FU Gongli XIAO Qi LI Xingpeng LIU Xiuyun ZHANG Daoyou GUO Xianghu WANG Zujun QIN | 2021 | Frontiers of Information Technology & Electronic Engineering2021，22，10： | 0 |
| 17 | Genome sequencing of Sitopsis species provides insights into their contribution to the B subgenome of bread wheat显示文摘Wheat(Triticum aestivum,BBAADD)is an allohexaploid species that originated from two polyploidization events.The progenitors of the A and D subgenomes have been identified as Triticum urartu and Aegilops tauschii,respectively.Current research suggests that Aegilops speltoides is the closest but not the direct ancestor of the B subgenome.However,whether Ae.speltoides has contributed genomically to the wheat B subgenome and which chromosome regions are conserved between Ae.speltoides and the B subgenome remain unclear.Here,we assembled a high-quality reference genome for Ae.speltoides,resequenced 53 accessions from seven species(Aegilops bicornis,Aegilops longissima,Aegilops searsii,Aegilops sharonensis,Ae.speltoides,Aegilops mutica[syn.Amblyopyrum muticum],and Triticumdicoccoides)and revealed their genomic contributions to the wheat B subgenome.Our results showed that centromeric regions were particularly conserved between Aegilops and Triticum and revealed 0.17 Gb of conserved blocks between Ae.speltoides and the B subgenome.We classified five groups of conserved and non-conserved genes between Aegilops and Triticum,revealing their biological characteristics,differentiation in gene expression patterns,and collinear relationships between Ae.speltoides and the wheat B subgenome.We also identified gene families that expanded in Ae.speltoides during its evolution and 789 genes specific to Ae.speltoides.These genes can serve as genetic resources for improvement of adaptability to biotic and abiotic stress.The newly constructed reference genome and large-scale resequencing data for Sitopsis species will provide a valuable genomic resource for wheat genetic improvement and genomic studies. | Yuxin Yang Licao Cui Zefu Lu Guangrong Li Zujun Yang Guangyao Zhao Chuizheng Kong Danping Li Yaoyu Chen Zhencheng Xie Zhongxu Chen Lichao Zhang Chuan Xia Xu Liu Jizeng Jia Xiuying Kong | 2023 | Plant Communications2023，4，4： | 0 |
| 18 | New PCR based markers allowed to identify Secale chromatin in wheat-Secale africanum introgression lines显示文摘The genus of Secale has many agronomically important characters.In order to use the best of this species,markers tracking the rye chromatin incorporated into wheat must be developed.In this study,one rye genome-specific random amplified polymorphic DNA(RAPD)marker was isolated from Secale africanum(Ra genome).Two cloned markers,named OPP13_(1165) and OPP13_(662),were 1165 bp and 662 bp,respectively.Sequence analysis revealed that OPP13_(1165) was highly homologous to a part of a new class of transposon-like gene called the Revolver family,and OPP13_(662) was partially similar to LTR gypsy-like retrotransposon.Fluorescence in situ hybridization(FISH)showed only OPP13_(1165) localized within the whole arms of rye except their terminal regions and no signal was detected on wheat chromosomes,while OPP13_(662) had no hybridization signal detected on wheat and rye genomes.Based on these sequences,two pairs of sequence-characterized amplified region(SCAR)primers were designed,and the resulted SCAR markers were able to target both cultivated and wild Secale species.The FISH patterns and the two SCAR markers should be able to identify and track all wheat-rye translocation lines,especially the S.africanum chromatin. | Juqing JIA Guangrong LI Cheng LIU Jianping ZHOU Zujun YANG | 2010 | Frontiers in Biology2010，5，2： | 0 |
| 19 | Novel expressed sequence tags of an alpine-cold plant species,Gymnadenia conopsea (Orchidaceae)显示文摘Gymnadenia conopsea,an alpine Orchidaceae plant,was one of the widely used Tibetan traditional medicines.In this study,we sequenced total 105 expressed sequence tags (ESTs) from a full-length cDNA expression library constructed by the Oligo-capping technique.The further bioinformatic analyses suggested that the 65 represented unique sequences showed high homology to previously identified genes in other plants:30 sequences matched to other uncharacterized expressed sequence tags (ESTs),and 10 sequences showed no good matches to available sequences in DNA databases.Gene ontology annotation by InterProScan indicated that many of these cDNAs (7 percent) have no known molecular functions and may be unique to G.conopsea.Fifty-five ESTs with matched proteins were involved in a series of diverse functions,in which molecular function such as 'binding' (42.9 percent) and 'catalytic activity' (25.0 percent) were the most frequent functions of the cDNAs.This cDNA library provided a critical basis for further investigation of functional genes expression under cold stress in this alpine species.In addition,13 ESTs-based polymerase chain reaction (PCR) primers were designed and can also be used for genotypic identification and for the genetic diversity analysis of G.conopsea and its closely related species. | JuQing Jia JianPing Zhou Tao Zhang ZhengNan Wen GuangRong Li ZuJun Yang | 2010 | Research in Cold and Arid Regions2010，2，2： | 0 |
| 20 | Haploidentical transplantation has a superior graft-versus-leukemia effect than HLA-matched sibling transplantation for Ph- high-risk B-cell acute lymphoblastic leukemia显示文摘Background: Compared with human leukocyte antigen (HLA)-matched sibling donor (MSD) transplantation, it remains unclear whether haploidentical donor (HID) transplantation has a superior graft-versus-leukemia (GVL) effect for Philadelphia-negative (Ph-) high-risk B-cell acute lymphoblastic leukemia (B-ALL). This study aimed to compare the GVL effect between HID and MSD transplantation for Ph- high-risk B-ALL.Methods: This study population came from two prospective multicenter trials (NCT01883180, NCT02673008). Immunosuppressant withdrawal and prophylactic or pre-emptive donor lymphocyte infusion (DLI) were administered in patients without active graft-versus-host disease (GVHD) to prevent relapse. All patients with measurable residual disease (MRD) positivity posttransplantation (post-MRD+) or non-remission (NR) pre-transplantation received prophylactic/pre-emptive interventions. The primary endpoint was the incidence of post-MRD+.Results: A total of 335 patients with Ph- high-risk B-ALL were enrolled, including 145 and 190, respectively, in the HID and MSD groups. The 3-year cumulative incidence of post-MRD+ was 27.2% (95% confidence interval [CI]: 20.2%-34.7%) and 42.6% (35.5%-49.6%) in the HID and MSD groups(P = 0.003), respectively. A total of 156 patients received DLI, including 60 (41.4%) and 96 (50.5%), respectively, in the HID and MSD groups (P= 0.096). The 3-year cumulative incidence of relapse was 18.6% (95% CI: 12.7%-25.4%) and 25.9% (19.9%-32.3%;P = 0.116) in the two groups, respectively. The 3-year overall survival (OS) was 67.4% (95% CI: 59.1%-74.4%) and 61.6% (54.2%-68.1%;P = 0.382), leukemia-free survival (LFS) was 63.4% (95% CI: 55.0%-70.7%) and 58.2% (50.8%-64.9%;P= 0.429), and GVHD-free/relapse-free survival (GRFS) was 51.7% (95% CI: 43.3%-59.5%) and 37.8% (30.9%-44.6%;P= 0.041), respectively, in the HID and MSD groups.Conclusion: HID transplantation has a lower incidence of post-MRD+ than MSD transplantation, suggesting that HID transplantation might have a superior GVL effect than MSD transplantation for Ph- high-risk B-ALL patients.Trial registration: ClinicalTrials.gov: NCT01883180, NCT02673008. | Fan Menglin Wang Yu Lin Ren Lin Tong Huang Fen Fan Zhiping Xu Yajing Yang Ting Xu Na Shi Pengcheng Nie Danian Lin Dongjun Jiang Zujun Wang Shunqing Sun Jing Huang Xiaojun Liu Qifa Xuan Li | 2022 | Chinese Medical Journal2022，，8： | 0 |
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