|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Dihydrocelastrol inhibits multiple myeloma cell proliferation and promotes apoptosis through ERK1/2 and IL-6/STAT3 pathways in vitro and in vivo显示文摘多重骨髓瘤(公里) 是第二很经常的恶意的 hematological 疾病。Dihydrocelastrol (DHCE ) 被 hydrogenated celastrol 综合,从中国药用的植物 Tripterygium regelii 孤立的 treterpene。在这研究,我们首先在公里房间上报导了 DHCE 的反肿瘤活动。我们发现 DHCE 能禁止房间增长并且在 vitro 通过 caspase 依赖的方法支持 apoptosis。另外, DHCE 能使 interleukin (IL ) 的表达式失去活性 -6 和 downregulate 细胞外的调整蛋白质 kinases (ERK1/2 ) 和信号变换器和在公里的抄写 3 的使活跃之物(STAT3 ) 的 phosphorylation。它也面对 IL-6 对公里房间线保留了它的活动。而且,有 DHCE 的公里房间的处理在房间周期的 G 0/G1 阶段导致了房间的累积。尤其是, DHCE 减少了 4 和 6 在公里房间衬里的 cyclin D1 和 cyclin 依赖的 kinases 的表示。另外,它向 MM 房间线的功效能与 histone deacetylase 禁止者 panobinostat (LBH589 ) 在联合被提高,它在公里作为潜在的治疗学的策略暗示了 DHCE 和 LBH589 的联合处理的可能性。另外,有 DHCE 的 NCI-H929 忍受肿瘤的裸体老鼠的处理(10 mg/kg/d, i.p, 1-14 天) 在 vivo 导致了肿瘤生长的 73% 抑制。一起拿,我们的现在的学习的结果显示 DHCE 能禁止细胞的增长并且在骨髓瘤房间导致房间 apoptosis 通过不同机制调停了,可能通过禁止 IL-6/STAT3 和 ERK1/2 小径。并且它可以为公里病人提供一种新治疗学的选择。 | Liangning Hu Huiqun Wu Bo Li Dongliang Song Guang Yang Gege Chen Bingqian Xie Zhijian Xu Yong Zhang Dandan Yu Jun Hou Wenqin Xiao Xi Sun Gaomei Chang Yiwen Zhang Lu Gao Bojie Dai Yi Tao Jumei Shi Weiliang Zhu | 2017 | Acta Biochimica et Biophysica Sinica2017，49，5： | 5 |
| 2 | Targeting the PI3K/Akt/mTOR signaling pathway by pterostilbene attenuates mantle cell lymphoma progression显示文摘 | Dandan Yu Yong Zhang Gege Chen Yongsheng Xie Zhijian Xu Shuaikang Chang Liangning Hu Bo Li Wenxuan Bu Yingcong Wang Wenqin Xiao Xi Sun Gaomei Chang Lu Gao Sujing Qiang Xiaosong Wu Weiliang Zhu Jumei Shi | 2018 | Acta Biochimica et Biophysica Sinica2018，50，8： | 4 |
| 3 | Efficacy of Sijunzi decoction(四君子汤) on limb weakness in spleen Qi deficiency model rats through adenosine monophosphate-activated protein kinase/unc-51 like autophagy activating kinase 1 signaling显示文摘OBJECTIVE:To investigate the efficacy of Sijunzi decoction(四君子汤)on limb weakness in a rat model of spleen Qi deficiency(SQD),and to study its effect on mitophagy in skeletal muscle through adenosine monophosphate-activated protein kinase(AMPK)/unc-51 like autophagy activating kinase 1(ULK1)signaling.METHODS:SQD model rats were produced by fasting combined with forced swimming method for15 d.After model assessment,rats were randomly divided into four groups of 10[low/middle/high(L/M/H)Sijunzi decoction dose groups and a normal saline(S)group].Limb holding power(HP)and body mass(BM)were measured after 2 weeks of treatment.Following euthanasia,quadriceps femoris were dissected and myofiber and mitochondrial morphology were observed by transmission electron microscopy(TEM).Mitochondrial membrane potential(MMP),adenosine triphosphatase(ATP)and reactive oxygen species(ROS)levels were determined using colorimetric methods,and immunoblot analysis of Microtubule-associated protein light chain 3(LC3)and Sequestosome 1(p62)was performed to monitor mitophagy and AMPK/ULK1 signaling.RESULTS:Compared with control(C)group rats,in the S group,HP was reduced,the myofiber Z line was disordered,mitochondria were scattered,and numerous vacuoles and mitophagy were observed.MMP and ATP levels were reduced,ROS levels were elevated,and LC3B expression,and p-AMPKα(Thr172)/AMPKα,p-ULK1(Ser555)/ULK1,and p-Raptor(Ser792)/Raptor ratios were increased,while p62 expression and p-mT OR(Ser2448)/mT OR and p-ULK1(Ser757)/ULK1 ratios were decreased.After treatment,compared with the S group,HP was improved in M and H groups but not in the L group.Mitophagy was reduced in M,H and L groups but the Z line was disordered and vacuolization remained in the L group.ATP levels were elevated in M,H and L groups,and MMPs were elevated in M and H groups but not in the L group.ROS levels were decreased in M,H and L groups,as were LC3B expression and p-Raptor(Ser792)/Raptor ratios,while p62 expression and p-mT OR(Ser2448)/mT OR and p-ULK1(Ser757)/ULK1 ratios were increased in M and H groups but not in the L group.p-AMPKα(Thr172)/AMPKαand p-ULK1(Ser555)/ULK1 ratios were decreased in M,H and L groups.CONCLUSIONS:Sijunzi decoction improved HP,possibly by inhibiting mitophagy via suppression of AMPK/ULK1 signaling.This restored mitochondrial morphology and improved oxidative phosphorylation,which contributed to recovery of limb weakness in SQD model rats. | LIU Wenjun XU Xinzhu DUAN Zhiyuan LIANG Xicai MA Dan LI Gege XIE Xin CHAI Jiyan CHEN Jing SHAN Dehong | 2021 | Journal of Traditional Chinese Medicine2021，41，4： | 2 |
| 4 | Antitumor effect of dobutamine on multiple myeloma via mitogen-activated protein kinase pathway in vitro显示文摘多重骨髓瘤(公里) 是最恶意的癌症之一并且被房间, monoclonal 免疫球蛋白的分泌物和 osteolytic 损害,和它的发生在中年、老的病人正在增加的血浆被骨头髓的渗入描绘[1 ] 。作为公里房间异构并且药抵抗、起始的公里通常进行到 relapsed/refractory 阶段。尽管象镇静药, bortezomib,和 lenalidomide 那样的存在治疗能延长公里病人的幸存,公里是是仍然考虑了医不好。因此,建立新有效治疗对待 relapsed/refractory 公里是必要的。 | Bingqian Xie Zhijian Xu Guang Yang Gege Chen Bo Li Liangning Hu Wenqin Xiao Xi Sun Minjie Gao Lu Gao Xiaosong Wu Yi Tao Weiliang Zhu Jumei Shi | 2016 | Acta Biochimica et Biophysica Sinica2016，48，12： | 1 |
| 5 | Development and Validation of a Nomogram for Prediction of the Risk of MAFLD in an Overweight and Obese Population显示文摘Background and Aims:Metabolic associated fatty liver disease(MAFLD)is a serious condition,and a simple meth-od is needed for practitioners to identify patients with the disease and have a high risk of disease progression.Meth-ods:We developed and validated a nomogram for fatty liver disease and reclassified the risk factors for MAFLD.The development cohort had 335 patients who received bioel-ectrical impedance analysis and liver ultrasound attenua-tion measurements at Shenzhen People’s Hospital between September 2020 and June 2021.The validation cohort had 200 patients from other hospitals who received the same evaluation.A random forest procedure and binary logistic analysis were used to screen for risk factors,establish a fatty liver disease predictive model,and forecast the risk of MAFLD.The performance of the nomogram was evaluated by measurement of discrimination,calibration,and clinical usefulness.Results:The nomogram provided good predic-tions in a model that included body mass index(BMI)and waist circumference.The areas under the curve of the nom-ogram were 0.793 in the development cohort and 0.774 in the validation cohort.The nomogram performed well for calibration,category-free net reclassification improvement,and integrated discrimination improvement.Decision curve analysis indicated the nomogram performed better than BMI for predicting net outcome.Conclusions:The nomo-gram was an effective screening tool for fatty liver disease,and for those overweight individuals,may help physicians make appropriate decisions regarding treatment of MAFLD. | Di Song Qian Ge Ming Chen Song Bai Xiaoshu Lai Gege Huang Mengmeng Liu Miaofang Lin Jinfeng Xu Fajin Dong | 2022 | Journal of Clinical and Translational Hepatology2022，10，6： | 1 |
| 6 | Distributed implantation of a flexible microelectrode array for neural recording显示文摘Flexible multichannel electrode arrays(fMEAs)with multiple flaments can be flexibly implanted in various patterns.It is necessary to develop a method for implanting the fMEA in different locations and at various depths based on the recording demands.This study proposed a strategy for reducing the microelectrode volume with integrated packaging.An implantation system was developed specifically for semiautomatic distributed implantation.The feasibility and convenience of the fMEA and implantation platform were verified in rodents.The acute and chronic recording results provied the effectiveness of the packaging and implantation methods.These methods could provide a novel strategy for developing fMEAs with more flaments and recording sites to measure functional interactions across multiple brain regions. | Chunrong Wei Yang Wang Weihua Pei Xinyong Han Longnian Lin Zhiduo Liu Gege Ming Ruru Chen Pingping Wu Xiaowei Yang Li Zheng Yijun Wang | 2022 | Microsystems & Nanoengineering2022，8，3： | 1 |
| 7 | Influence of nano-mechanical evolution of Ti_(3)AlC_(2) ceramic on the arc erosion resistance of Ag-based composite electrical contact material显示文摘Al-containing MAX phase ceramic has demonstrated great potential in the field of high-performance low-voltage electrical contact material.Elucidating the anti-arc erosion mechanism of the MAX phase is crucial for further improving performance,but it is not well-understood.In this study,Ag/Ti_(3)AlC_(2) electrical contact material was synthesized by powder metallurgy and examined by nanoindentation techniques such as constant loading rate indentation,creep testing,and continuous stiffness measurements.Our results indicated a gradual degradation in the nano-mechanical properties of the Ti_(3)AlC_(2) reinforcing phase with increasing arc erosion times,although the rate of this degradation appeared to decelerate over arc erosion times.Specifically,continuous stiffness measurements highlighted the uneven mechanical properties within Ti_(3)AlC_(2),attributing this heterogeneity to the phase’s decomposition.During the early(1-100 times)and intermediate(100-1000 times)stages of arc erosion,the decline in the nano-mechanical properties of Ti_(3)AlC_(2) was primarily ascribed to the decomposition of Ti_(3)AlC_(2) and limited surface oxidation.During the later stage of arc erosion(1000-6200 times),the inner region of Ti_(3)AlC_(2) also sustained arc damage,but a thick oxide layer formed on its surface,enhancing the mechanical properties and overall arc erosion resistance of the Ag/Ti_(3)AlC_(2). | Xuelian Wu Chengzhe Wu Xinpeng Wei Wanjie Sun Chengjian Ma Yundeng Zhang Gege Li Liming Chen Dandan Wang Peigen Zhang Zhengming Sun Jianxiang Ding | 2024 | Journal of Advanced Ceramics2024，13，2： | 0 |
| 8 | Determination of Triazine Herbicides in Environmental Water Samples by Acetonitrile Inorganic Salt Aqueous Two-Phase Microextraction System显示文摘Acetonitrile inorganic salt aqueous two-phase extraction method was established for the determination of triazine herbicides(simeton,cyanazine,desmetryn,terbumeton,terbuthylazine and dimethametryn)in environmental water samples by high-performance liquid chromatography(HPLC).The extraction solvents types and volume of acetonitrile,the inorganic salt types and amount of(NH_(4))_(2)SO_(4),extraction time and pH of sample solution were optimized by single-factor experiment and central composite design.Under the optimum extraction conditions,all linear ranges were obtained with coefficients of determination(r)≥0.9993.The limits of detection for this proposed method were in the range of 0.16-0.28μg/L for six triazine herbicides.The developed method has been successfully applied to the analysis of target triazine herbicides from lake,canal and moat in real-world water samples.The recoveries of target analytes were in the range from 87.0 to 110.9%and the relative standard deviation was lower than 7.3%. | Shiqian Gao Gege Wu Xiaomeng Li Jie Chen Youyi Wu Junxia Wang Zhanen Zhang | 2018 | Journal of Analysis and Testing2018，2，4： | 0 |
| 9 | Novel cyclophosphamide of natural products osalmide and pterostilbene induces cytotoxicity and cell cycle arrest in diffuse large B-cell lymphoma cells显示文摘Diffuse large B-cell lymphoma(DLBCL)is the most common category and disease entity of non-Hodgkin lymphoma.Osalmide and pterostilbene are natural products with anticancer activities via different mechanism.In this study,using a new synthetic strategy for the two natural products,we obtained the compound DCZ0801,which was previously found to have anti-multiple myeloma activity.We performed both in vitro and in vivo assays to investigate its bioactivity and explore its underlying mechanism against DLBCL cells.The results showed that DCZ0801 treatment gave rise to a dose-and time-dependent inhibition of cell viability as determined by CCK-8 assay and flow cytometry assay.Western blot analysis results showed that the expression of caspase-3,caspase-8,caspase-9 and Bax was increased,while BCL-2 and BCL-XL levels were decreased,which suggested that DCZ0801 inhibited cell proliferation and promoted intrinsic apoptosis.In addition,DCZ0801 induced G0/G1 phase arrest by downregulating the protein expression levels of CDK4,CDK6 and cyclin D1.Furthermore,DCZ0801 exerted an anti-tumor effect by down-regulating the expressions of p-PI3K and p-AKT.There also existed a trend that the expression of p-JNK and p-P38 was restrained.Intraperitoneal injection of DCZ0801 suppressed tum or development in xenograft mouse models.The preliminary metabolic study showed that DCZ0801 displayed a rapid metabolism within 30 min.These results demonstrated that DCZ0801 may be a new potential anti-DLBCL agent in DLBCL therapy. | Mengyu Xi Wan He Bo Li Jinfeng Zhou Zhijian Xu Huiqun Wu Yong Zhang Dongliang Song Liangning Hu Ye Lu Wenxuan Bu Yuanyuan Kong Gege Chen Shuaikang Chang Jumei Shi Weiliang Zhu | 2020 | Acta Biochimica et Biophysica Sinica2020，52，4： | 0 |
| 10 | The multifaceted role of placental growth factor in the pathogenesis and progression of bronchial asthma and pulmonary fibrosis:Therapeutic implications显示文摘Placental growth factor(PlGF)is a glycosylated dimeric protein that is homologous to vascular endothelial growth factor(VEGF).PlGF expression is upregulated in patients with bronchial asthma,suggesting that it plays a role in the pathogenesis of asthma.Bronchial asthma is characterized by chronic airway inflammation and airway hyperresponsiveness(AHR).After recurrent asthma attacks,pulmonary fibrosis develops and leads to airway remo-deling and a further decline in lung function.In this review,we focused on the pivotal role of PlGF in chronic airway inflammation,AHR,and airway remodeling during bronchial asthma.Furthermore,we summarized data showing that PlGF may be a potential therapeutic target in bronchial asthma. | Dan Huang Gege Liu Zhiyi Xu Shushu Chen Cuili Wang Dewei Liu Jiahao Cao Junfen Cheng Bin Wu Dong Wu | 2023 | Genes & Diseases2023，10，4： | 0 |
| 11 | Huoxue Jiedu Huayu recipe(活血解毒化瘀方)alleviates contralateral renal fibrosis in unilateral ureteral obstruction rats by inhibiting the transformation of macrophages to myofibroblast显示文摘OBJECTIVE:To investigate the action and underlying mechanisms of Huoxue Jiedu Huayu recipe(活血解毒化瘀方,HJHR)against unilateral ureteral obstruction(UUO)-induced injury in the contralateral kidney.METHODS:Forty-eight male Sprague-Dawley rats weighing(200±10)g were used in this study and randomly assigned to 4 groups:a sham group,a UUO group,a UUO+eplerenone(EPL)group,and a UUO+HJHR group.The contralateral kidneys were harvested for further study 180 d after surgery.Histological analysis,immunohistochemistry and immunofluorescence were used to study the fibrosis of the contralateral kidneys obtained from UUO rats.Contralateral kidney damagerelated pathway proteins were detected by real-time polymerase chain reaction and Western blot analysis.RESULTS:HJHR significantly inhibited fibrosis of the contralateral kidney in UUO rats by attenuating the UUOinduced macrophage-to-myofibroblast transition(MMT)in the contralateral kidney.Moreover,HJHR attenuated fibrosis in the contralateral kidney of UUO rats by preventing MMT through the aldosterone/mineralocorticoid receptor/serum/glucocorticoid regulated kinase 1 pathway.CONCLUSIONS:Our findings suggest that HJHR may be a potential treatment for renal interstitial fibrosis of obstructive nephropathy. | XIONG Yunzhao LIU Lingjin LIU Ziqian CHEN Gege HAO Juan GAO Xiaomeng QIANG Panpan WANG Zheng XU Qingyou | 2023 | Journal of Traditional Chinese Medicine2023，43，1： | 0 |
| 12 | antitumor activity in bortezomib-resistant multiple myeloma cells through inhibition of JAK2/STAT3 pathway显示文摘Multiple myeloma(MM),the second most common haematological malignancy,is currently incurable because patients often develop multiple drug resistance and experience subsequent relapse of the disease.This study aims to identify a potential therapeutic agent that can counter bortezomib(BTZ)resistance in MM.DCZ0358,a novel alkaloid compound,is found to exert potent cytotoxic effects against BTZ-resistant MM cells in vivo and in vitro.The antimyeloma activity of DCZ0358 is associated with inhibition of cell proliferation,promotion of cell apoptosis via caspase-mediated apoptotic pathways,and induction of G0/G1 phase arrest via downregulation of cyclin D1,CDK4,and CDK6.Further investigation of the molecular mechanism shows that DCZ0358 suppresses the JAK2/STAT3 signaling pathway.In conclusion,DCZ0358 can successfully counter BTZ resistance in MM cells.This study provides evidence that warrants future preclinical assessments of DCZ0358 as a therapeutic agent against BTZ resistance in MM. | Bibo Zhang Bo Li Yongsheng Xie Shuaikang Chang Zhijian Xu Huifang Hu Gege Chen Ting Zhang Jun He Xiaosong Wu Huabin Zhu Weiming Lai Dongliang Song Ying Lu Xinyan Jia Weiliang Zhu Jumei Shi | 2023 | Acta Biochimica et Biophysica Sinica2023，55，2： | 0 |
| 13 | A novel silicone derivative of natural osalmid(DCZ0858)induces apoptosis and cell cycle arrest in diffuse large B-cell lymphoma via the JAK2/STAT3 pathway显示文摘Diffuse large B-cell lymphoma(DLBCL)is a highly heterogeneous malignant tumor characterized by diffuse growth.DCZ0858 is a novel small molecule with excellent antitumor effects in DLBCL.This study explored in depth the inhibitory effect of DCZ0858 on DLBCL cell lines.Cell Counting Kit-8(CCK-8)and plate colony formation assays were used to evaluate cell proliferation levels.Flow cytometry was employed to analyze apoptosis and the cell cycle,and western blotting was used to quantify the expression of cell cycle regulators.The results indicated that DCZ0858 inhibited cell growth in a concentration-dependent and time-dependent manner while inducing no significant toxicity in normal cells.Moreover,DCZ0858 initiated cell apoptosis via both internal and external apoptotic pathways.DCZ0858 also induced cell cycle arrest in the G0/G1 phase,thereby controlling cell proliferation.Further investigation of the molecular mechanism showed that the JAK2/STAT3 pathway was involved in the DCZ0858-mediated antitumor effects and that JAK2 was the key target for DCZ0858 treatment.Knockdown of JAK2 partly weakened the DCZ0858-mediated antitumor effect in DLBCL cells,while JAK2 overexpression strengthened the effect of DCZ0858 in DLBCL cells.Moreover,a similar antitumor effect was observed for DCZ0858 and the JAK2 inhibitor ruxolitinib,and combining the two could significantly enhance cancer-suppressive signaling.Tumor xenograft models showed that DCZ0858 inhibited tumor growth in vivo and had low toxicity in important organs,findings that were consistent with the in vitro data.In summary,DCZ0858 is a promising drug for the treatment of DLBCL. | Kang Lu Bo Li Hui Zhang Zhijian Xu Dongliang Song Lu Gao Haiguo Sun Liping Li Yingcong Wang Qilin Feng Gege Chen Liangning Hu Rong Wei Yongsheng Xie Dandan Yu Xiaosong Wu Weiliang Zhu Jumei Shi | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 0 |
| 14 | Surface engineering of carbon dots for highly sensitive α-glucosidase assay and inhibition evaluation显示文摘Monitoringα-glucosidase(α-Glu)activity is of great significance for the early diagnosis of typeⅡdiabetes.Here the blue fluorescent carbon dots(CDs)were integrated with two different recognizing molecules,β-cyclodextrin and phenylboronic acid,for assembling a multifunctional CDs(mCDs)nanoplatform for sensitively analyzingα-Glu and its inhibitors.The hydrolyzed product of 4-nitrophenyl-α-D-glucopyranoside(α-Glu substrate),p-nitrophenol,could efficiently quench the fluorescence of mCDs due to its cooperative molecular recognition withβ-cyclodextrin and phenylboronic acid.The mCDs could be utilized for the detection ofα-Glu activity with the limit of detection of 0.030 U/L.Moreover,the presentα-Glu detection platform revealed a high selectivity,and other natural enzymes showed scarcely any effect on the present mCDs system.The proposed method could be facilely used to screenα-Glu inhibitors with satisfying performance.The rational mCDs is expected to supplement more comprehensive biosensing platforms for highly sensitive and specific recognition of disease-relevant biomarkers with clinical importance. | Meijuan Liang Gege Song Yeqing Wan Yingying Chen Fuan Wang Xiaoqing Liu | 2024 | Chinese Chemical Letters2024，35，3： | 0 |
| 15 | Dihydrocelastrol induces antitumor activity and enhances the sensitivity of bortezomib in resistant multiple myeloma by inhibiting STAT3-dependent PSMB5 regulation显示文摘Multiple myeloma(MM)is characterized by excessive aggregation of B-cell-derived malignant plasma cells in the hematopoietic system of bone marrow.Previously,we synthesized an innovative molecule named dihydrocelastrol(DHCE)from celastrol,a triterpene purified from medicinal plant Tripterygium wilfordii.Herein,we explore the therapeutic properties and latent signal transduction mechanism of DHCE action in bortezomib(BTZ)-resistant(BTZ-R)MM cells.In this study,we first report that DHCE shows antitumor activities in vitro and in vivo and exerts stronger inhibitory effects than celastrol on BTZ-R cells.We find that DHCE inhibits BTZ-R cell viability by promoting apoptosis via extrinsic and intrinsic pathways and suppresses BTZ-R MM cell proliferation by inducing G0/G1 phase cell cycle arrest.In addition,inactivation of JAK2/STAT3 and PI3K/Akt pathways are involved in the DHCE-mediated antitumor effect.Simultaneously,DHCE acts synergistically with BTZ on BTZ-R cells.PSMB5,a molecular target of BTZ,is overexpressed in BTZ-R MM cells compared with BTZ-S MM cells and is demonstrated to be a target of STAT3.Moreover,DHCE downregulates PSMB5 overexpression in BTZ-R MM cells,which illustrates that DHCE overcomes BTZ resistance through increasing the sensitivity of BTZ in resistant MM via inhibiting STAT3-dependent PSMB5 regulation.Overall,our findings imply that DHCE may become a potential therapeutic option that warrants clinical evaluation for BTZ-R MM. | Shuhan Jin Bo Li Bibo Zhang Xuejie Gao Xinyan Jia Li Xu Shuaikang Chang Ke Hu Guanli Wang Zhijian Xu Ting Zhang Dongliang Song Guang Yang Xiaosong Wu Huabin Zhu Cheng Huang Yumeng Lu Jumei Shi Weiliang Zhu Gege Chen | 2023 | Acta Biochimica et Biophysica Sinica2023，55，12： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员