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| 1 | Clinical and biochemical indexes from 2019-nCoV infected patients linked to viral loads and lung injury显示文摘The outbreak of the 2019-nCoV infection began in December 2019 in Wuhan,Hubei province,and rapidly spread to many provinces in China as well as other countries.Here we report the epidemiological,clinical,laboratory,and radiological characteristics,as well as potential biomarkers for predicting disease severity in 2019-nCoV-infected patients in Shenzhen,China.All 12 cases of the 2019-nCoV-infected patients developed pneumonia and half of them developed acute respiratory distress syndrome (ARDS).The most common laboratory abnormalities were hypoalbuminemia,lymphopenia,decreased percentage of lymphocytes (LYM) and neutrophils (NEU),elevated C-reactive protein (CRP) and lactate dehydrogenase (LDH),and decreased CD8 count.The viral load of 2019-nCoV detected from patient respiratory tracts was positively linked to lung disease severity.ALB,LYM,LYM (%),LDH,NEU (%),and CRP were highly correlated to the acute lung injury.Age,viral load,lung injury score,and blood biochemistry indexes,albumin (ALB),CRP,LDH,LYM (%),LYM,and NEU (%),may be predictors of disease severity.Moreover,the Angiotensin Ⅱlevel in the plasma sample from 2019-nCoV infected patients was markedly elevated and linearly associated to viral load and lung injury.Our results suggest a number of potential diagnosis biomarkers and angiotensin receptor blocker (ARB) drugs for potential repurposing treatment of 2019-nCoV infection. | Yingxia Liu Yang Yang Cong Zhang Fengming Huang Fuxiang Wang Jing Yuan Zhaoqin Wang Jinxiu Li Jianming Li Cheng Feng Zheng Zhang Lifei Wang Ling Peng Li Chen Yuhao Qin Dandan Zhao Shuguang Tan Lu Yin Jun Xu Congzhao Zhou Chengyu Jiang Lei Liu | 2020 | Science China(Life Sciences)2020，63，3： | 157 |
| 2 | A retrospective of knowledge graphs显示文摘 | Jihong YAN Chengyu WANG Wenliang CHENG Ming GAO Aoying ZHOU | 2018 | Frontiers of Computer Science2018，12，1： | 25 |
| 3 | Herbal decoctosome is a novel form of medicine显示文摘Traditionally, herbal medicine is consumed by drinking decoctions produced by boiling herbs with water. The functional components of the decoction are heat stable. Small RNAs(sRNAs) were reported as a new class of functional components in decoctions. However, the mechanisms by which sRNAs survive heat treatment of the decoction and enter cells are unclear.Previous studies showed that plant-derived exosome-like nanoparticles(ELNs), which we call botanosomes, could deliver therapeutic reagents in vivo. Here, we report that heat-stable decoctosomes(ELNs) from decoctions have more therapeutic effects than the decoctions in vitro and demonstrate therapeutic efficacy in vivo. Furthermore, sRNAs, such as HJT-sRNA-m7 and PGY-sRNA-6, in the decoctosome exhibit potent anti-fibrosis and anti-inflammatory effects, respectively. Decoctosome is comprised of lipids, chemical compounds, proteins, and s RNAs. A medical decoctosome mimic is called bencaosome. A single lipid sphinganine(d22:0) identified in the decoctosome was mixed and heated with the synthesized sRNAs to form the simplest bencaosome. This simple bencaosome structure was identified by critical micelle concentration(cmc) assay that sRNAs coassembled with sphinganine(d22:0) to form the lipid layers of vesicles. The heating process facilitates co-assembly of sRNAs and sphinganine(d22:0) until a steady state is reached. The artificially produced sphinganine-HJT-sRNA-m7 and sphinganinePGY-sRNA-6 bencaosomes could ameliorate bleomycin-induced lung fibrosis and poly(I:C)-induced lung inflammation, respectively, following oral administration in mice. Our study not only demonstrates that the herbal decoctosome may represent a combinatory remedy in precision medicine but also provides an effective oral delivery route for nucleic acid therapy. | Xiaoyun Li Zhu Liang Jianchao Du Zhiqing Wang Song Mei Zhiqing Li Yan Zhao DANDan Zhao Yiming Ma Jun Ye Jiantao Xu Yu Zhao Jiahui Chang Yuhao Qin Lanlan Yu Chenxuan Wang Chengyu Jiang | 2019 | Science China(Life Sciences)2019，62，3： | 26 |
| 4 | IL-17 response mediates acute lung injury induced by the 2009 Pandemic Influenza A (HIN1) Virus显示文摘 | Chenggang Li Penghui Yang Yang Sun Taisheng Li Chen Wang Zhong Wang Zhen Zou Yiwu Yan Wei Wang Chen Wang Zhongwei Chen Li Xing Chong Tang Xiangwu Ju Feng Guo Jiejie Deng Yan Zhao Peng Yang Jun Tang Huanling Wang Zhongpeng Zhao Zhinan Yin Bin Cao Xiliang Wang Chengyu Jiang | 2012 | Cell Research2012，22，3： | 22 |
| 5 | Cationic nanocarriers induce cell necrosis through impairment of Na^+/K^+-ATPase and cause subsequent inflammatory response显示文摘有积极表面费用的 Nanocarriers 以他们限制了他们的临床的应用的毒性著名。位于他们的毒性下面的机制例如煽动性的反应的正式就职,仍然保持大部分未知。在我们发现了 cationic nanocarriers 的那注射的现在的学习,包括 cationic liposomes, PEI,和 chitosan,导致了坏死的房间的快速的外观。cationic nanocarriers 导致的房间坏死依赖于他们的积极表面费用,但是不要求 RIP1 和 Mlkl。相反,细胞内部的 Na + 超载被发现伴随房间死亡。在文化媒介或有 Na +/K+-ATPase 阳离子绑定地点禁止者 ouabain 的房间的预告的处理的 Na + 的弄空,从房间坏死的保护的房间。而且,有 cationic nanocarriers 的处理在 vitro 并且在 vivo 禁止了 Na +/K+-ATPase 活动。计算模拟证明 cationic 搬运人能与 Na +/K+-ATPase 的阳离子绑定地点交往。有 ouabain 的小剂量的老鼠 pretreated 在 cationic nanocarriers 的致命的剂量的注射以后显示出改进幸存。进一步的分析建议 cationic nanocarriers 和 mitochondrial DNA 的产生的漏导致的那房间坏死能在 vivo 触发严重发炎,它被包含 TLR9 并且 MyD88 发信号的一条小径调停。一起拿,我们的结果揭示由此的新奇机制 cationic nanocarriers 通过和 mitochondrial 的随后的暴露的和 Na +/K+-ATPase, 的相互作用导致尖锐房间坏死是的联系损坏的分子的模式调停的一个关键事件煽动性的回答。我们的学习为评估 nanocarriers 的 biocompatibility 并且为药交货设计更好、更安全的有重要含意。 | Xiawei Wei Bin Shao Zhiyao He Tinghong Ye Min Luo Yaxiong Sang Xiao Liang Wei Wang Shuntao Luo Shengyong Yang Shuang Zhang Changyang Gong Maling Gou Hongxing Deng Yinglan Zhao Hanshuo Yang Senyi Deng Chengjian Zhao Li Yang Zhiyong Qian Jiong Li Xun Sun Jiahuai Han Chengyu Jiang Min Wu Zhirong Zhang | 2015 | Cell Research2015，25，2： | 21 |
| 6 | Plant-derived phosphocholine facilitates cellular uptake of anti-pulmonary fibrotic HJT-sRNA-m7显示文摘Pulmonary fibrosis, a progressive chronic disease with a high mortality rate, has limited treatment options. Currently, lung transplantation remains the only effective treatment. Here we report that a small RNA, HJT-sRNA-m7, from a Chinese herbal medicine Hong Jing Tian(HJT, RHODIOHAE CRENULATAE RADIX ET RHIZOMA, Rhodiola crenulata) can effectively reduce the expressions of fibrotic hallmark genes and proteins both in alveolar in vitro and in mouse lung tissues in vivo. We also discovered over one hundred oil-soluble chemicals from HJT decoctions, most of which are found in lipid extracts from other Chinese herbals decoctions, including Pu Gong Ying(PGY, TARAXACI HERBA, Taraxacum mongolicum), Chuan Xin Lian(CXL, changed to 'ANDROGRAPHIS HERBA, Andrographis paniculata'), and Jin Yin Hua(JYH, lonicera japonica or Honeysuckle). We identified the active component in these decoctions as two forms of phosphocholines, PC(18:0/18:2) and PC(16:0/18:2). These PCs potentially could form liposomes with small RNAs to enter human alveolar and gastric cells. Our experimental results suggest an unprecendent lipid complex route through which botanic small RNA can enter human bodies.Our results provide an innovative treatment strategy for oral delivery of siRNAs as therapeutic medication. | Jianchao Du Zhu Liang Jiantao Xu Yan Zhao Xiaoyun Li Yanli Zhang DANDan Zhao Ruxuan Chen Yang Liu Trupti Joshi Jiahui Chang Zhiqing Wang Yanxu Zhang Jindong Zhu Qiang Liu Dong Xu Chengyu Jiang | 2019 | Science China(Life Sciences)2019，62，3： | 21 |
| 7 | Comparison of simple discectomy and instrumented posterior lumbar interbody fusion for treatment of lumbar disc herniation combined with Modic endplate changes显示文摘 | Cao Peng Chen Zhe Zheng Yuehuan Wang Yuren Jiang Leisheng Yang Yaoqi Zhuang Chengyu Liang Yu Zheng Tao Gong Yaocheng Zhang Xingkai Wu Wenjian Qiu Shijing | 2014 | Chinese Medical Journal2014，，15： | 15 |
| 8 | Influenza A virus H5N1 entry into host cells is through clathrin-dependent endocytosis显示文摘Influenza A virus H5N1 presents a major threat to human health. The entry of influenza virus into host cells is believed to be mediated by hemagglutinin (HA), a virus surface glycoprotein that can bind terminal sialic acid residues on host cell glycoproteins and glycolipids. In this study, we elucidated the pathways through which H5N1 enters human lung carcinoma cell line A549. We first proved that H5N1 can enter A549 cells via endocytosis, as lysosomotropic agents, such as bafilomycin A1 and chloroquine, can rescue H5N1-induced A549 cell death. By using specific inhibitors, and siRNAs that target the clathrin pathway, we further found that H5N1 could enter A549 cells via clathrin-mediated endocytosis, while inhibitors targeting caveolae-mediated endocytosis could not inhibit H5N1 cell entry. These findings expand our understanding of H5N1 pathogenesis and provide new information for anti-viral drug research. | WANG HongLiang & JIANG ChengYu National Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100005, China | 2009 | Science China(Life Sciences)2009，52，5： | 11 |
| 9 | Effects of resolvin D1 on inflammatory responses and oxidative stress of lipopolysaccharide-induced acute lung injury in mice显示文摘 | Yuan Ruixia Wang Lei Yao Chengyue Wu Qingping Marie Christelle Xie Wanli Zhang Xingcai Sun Wei Wang Huiqing Yao Shanglong | 2014 | Chinese Medical Journal2014，，5： | 10 |
| 10 | Chemical and Isotopic Characteristics of the Water and Suspended Particulate Materials in the Yangtze River and Their Geological and Environmental Implications显示文摘The chemical and isotopic characteristics of the water and suspended particulate materials(SPM) in the Yangtze River were investigated on the samples collected from 25 hydrological monitoring stations in the mainsteam and 13 hydrological monitoring stations in the major tributaries during 2003 to 2007. The water samples show a large variation in both δD( 30‰ to 112‰) and δ18O( 3.8‰ to 15.4‰) values. Both δD and δ18O values show a decrease from the river head to the Jinsha Jiang section and then increase downstream to the river mouth. It is found that the oxygen and hydrogen isotopic compositions of the Yangtze water are controlled by meteoric precipitation, evaporation, ice(and snow) melting and dam building. The Yangtze SPM concentrations show a large variation and are well corresponded to the spatial and temporal changes of flow speed, runoff and SPM supply, which are affected by the slope of the river bed, local precipitation rate, weathering intensity, erosion condition and anthropogenic activity. The Yangtze SPM consists of clay minerals, clastic silicate and carbonate minerals, heavy minerals, iron hydroxide and organic compounds. From the upper to lower reaches, the clay and clastic silicate components in SPM increase gradually, but the carbonate components decrease gradually, which may reflect changes of climate and weathering intensity in the drainage area. Compared to those of the upper crust rocks, the Yangtze SPM has lower contents of SiO2, CaO, K2 O and Na2 O and higher contents of TFe2 O3 and trace metals of Co, Ni, Cu, Zn, Pb and Cd. The ΣREE in the Yangtze SPM is also slightly higher than that of the upper crust. From the upper to lower reaches, the CaO and MgO contents in SPM decrease gradually, but the SiO2 content increases gradually, corresponding to the increase of clay minerals and decrease of the carbonates. The δ30SiSPM values( 1.1‰ to 0.3‰) of the Yangtze SPM are similar to those of the average shale, but lower than those of the granite rocks( 0.3‰ to 0.3‰), reflecting the effect of silicon isotope fractionation in silicate weathering process. The δ30SiSPM values of the Yangtze SPM show a decreasing trend from the upper to the middle and lower reaches, responding to the variation of the clay content. The major anions of the river water are HCO 3, SO 4 2, Cl, NO 3, SiO 4 4 and F and the major cations include Ca2+, Na+, Mg2+, K+ and Sr2+. The good correlation between HCO3-content and the content of Ca2+may suggest that carbonate dissolution is the dominate contributor to the total dissolved solid(TDS) of the Yangtze River. Very good correlations are also found among contents of Cl, SO4 2, Na+, Mg2+, K+and Sr2+, indicating the important contribution of evaporite dissolution to the TDS of the Yangtze River. High TDS contents are generally found in the head water, reflecting a strong effect of evaporation in the Qinghai-Tibet Plateau. A small increase of the TDS is generally observed in the river mouth, indicating the influence of tidal intrusion. The F and NO3 contents show a clear increase trend from the upstream to downstream, reflecting the contribution of pesticides and fertilizers in the Chuan Jiang section and the middle and lower reaches. The DSi shows a decrease trend from the upstream to downstream, reflecting the effect of rice and grass growth along the Chuan Jiang section and the middle and lower reaches. The dissolved Cu, Zn and Cd in the Yangtze water are all higher than those in world large rivers, reflecting the effect of intensive mining activity along the Yangtze drainage area. The Yangtze water generally shows similar REE distribution pattern to the global shale. The δ30SiDiss values of the dissolved silicon vary from 0.5‰ to 3.7‰, which is the highest among those of the rivers studied. The δ30SiDiss values of the water in the Yangtze mainsteam show an increase trend from the upper stream to downstream. Its DSi and δ30SiDiss are influenced by multiple processes, such as weathering process, phytolith growth in plants, evaporation, phytolith dissolution, growth of fresh water diatom, adsorption and desorption of aqueous monosilicic acid on iron oxide, precipitation of silcretes and formation of clays coatings in aquifers, and human activity. The δ34SSO4 values of the Yangtze water range from 1.7‰ to 9.0‰. The SO4 in the Yangtze water are mainly from the SO4 in meteoric water, the dissolved sulfate from evaporite, and oxidation of sulfide in rocks, coal and ore deposits. The sulfate reduction and precipitation process can also affect the sulfur isotope composition of the Yangtze water. The87Sr/86Sr ratios of the Yangtze water range from 0.70823 to 0.71590, with an average value of 0.71084. The87Sr/86Sr ratio and Sr concentration are primary controlled by mixing of various sources with different87Sr/86Sr ratios and Sr contents, including the limestone, evaporite and the silicate rocks. The atmospheric precipitation and anthropogenic inputs can also contribute some Sr to the river. The δ11B values of the dissolved B in the Yangtze water range from 2.0‰ to 18.3‰, which is affected by multifactors, such as silicate weathering, carbonate weathering, evaporite dissolution, atmospheric deposition, and anthropogenic inputs. | DING Tiping GAO Jianfei TIAN Shihong SHI Guoyu CHEN Feng WANG Chengyu LUO Xurong HAN Dan | 2014 | Acta Geologica Sinica(English Edition)2014，88，1： | 10 |
| 11 | Expression and subcellular localization of mechano-growth factor in osteoblasts under mechanical stretch显示文摘Mechano-growth factor (MGF) is a stretch sensitive factor in myocytes, and it might also be produced by other mechanocytes under mechanical stimulation. In this study, both the mRNA and protein expression of MGF were detected in stretched osteoblasts. Quantitative analysis showed that a cyclic stretching stimulation caused a quick and sharp increase of MGF mRNA and protein expression from a low basal level under no stretch; the mRNA and protein levels respectively peaked in 6 and 12 h to 5 and 5.2 fold over the basal level and returned to normal by 24 h. The subcellular distribution of MGF protein was revealed by immunofluorescence analysis to be restricted to the nucleus. We concluded that cyclic stretching stimulation could induce MGF expression in osteoblasts in a pulsing fashion; and the nuclear distribution of MGF suggested that MGF might act in mechanocytes as an autocrine growth factor. | ZHANG BingBing, XIAN ChengYu, LUO YanFeng & WANG YuanLiang Research Center of Bioinspired Materials Science and Engineering, Bioengineering College, Chongqing University, Chongqing 400030, China | 2009 | Science China(Life Sciences)2009，52，10： | 10 |
| 12 | Avian influenza H5N1:an update on molecular pathogenesis显示文摘Avian influenza A virus constitutes a large threat to human health. Recent outbreaks of highly pathogenic avian influenza H5N1 virus in poultry and in humans have raised concerns that an influenza pandemic will occur in the near future. Transmission from avian species to humans remains sporadic, but the mortality associated with human infection is very high (about 62%). To date, there are no effective therapeutic drugs or a prophylactic vaccines available, which means that there is still a long way to go before we can eradicate or cure avian influenza. This review focuses on the molecular pathogenesis of avian influenza H5N1 virus infection. An understanding of the viral pathogenesis may facilitate the development of novel treatments or effective eradication of this fatal disease. | WANG HongLiang & JIANG ChengYu National Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100005, China | 2009 | Science China(Life Sciences)2009，52，5： | 9 |
| 13 | Effect of magnesium ions/Type I collagen promote the biological behavior of osteoblasts and its mechanism显示文摘Type I collagen(Col I)is a main component of extracellular matrix(ECM).Its safety,biocompatibility,hydrophilicity and pyrogen immunogenicity make it suitable for tissues engineering applications.Mg2t also control a myriad of cellular processes,including the bone development by enhancing the attachment and differentiation of osteoblasts and accelerating mineralization to enhance bone healing.In our studies,Mg2t bind collagen to promote the proliferation and differentiation of osteoblasts through the expression of integrins and downstream signaling pathways.In order to clarify the biological behavior effect of 10mM Mg2t/Col I coating,we performed 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT),alkaline phosphatase(ALP),406-diamidino-2-phenylindole(DAPI),Alizarin red staining and Rhodamine B-isothiocyanate(RITC)-labeled phalloidin experiments and found that 10mM Mg2t group,Col I-coating group,10mM Mg2t/Col I-coating group,respectively,promoted the proliferation and differentiation of osteoblasts,especially 10mM Mg2t/Col I-coating group.We detected the mRNA expression of osteogenic-related genes(Runx2,ALP and OCN,OPN and BMP-2)and the protein expression of signaling pathway(integrin a2,integrin b1,FAK and ERK1/2),these results indicated that 10mM Mg2t/Col I coating play an critical role in up-regulating the MC3T3-E1 cells activity.The potential mechanisms of this specific performance may be through activating via integrin a2b1-FAK-ERK1/2 protein-coupled receptor pathway. | Xiaojing Nie Xirao Sun Chengyue Wang Jingxin Yang | 2020 | Regenerative Biomaterials2020，7，1： | 8 |
| 14 | Alternative splicing and expression of the insulin-like growth factor (IGF-1) gene in osteoblasts under mechanical stretch显示文摘(IGF-1 ) 像胰岛素的生长因素 1 支持造骨细胞区别和骨头形成,和它的表示被机械段导致,因此, IGF-1 被认为连接机械刺激和本地织物回答的一个受动器分子。在这研究,一台机械拉长设备被设计把生理的水平静电干扰或周期的拉长刺激用于造骨细胞。IGF-1 mRNA 的不同 isoforms 被 RT-PCR 用各自的教材从房间放大,这些放大产品被定序。产品被发现被造骨细胞有选择地在拉长刺激下面生产的拼接的 IGF-1 的 iso 形式。ThisIGF-1 isoform 与原来在肌肉细胞被识别的 mechano 生长因素(MGF ) 有相同顺序。肝类型 IGF (L.IGF-1 ) 和在在段刺激下面的造骨细胞的 MGF 的表示的规定进一步用半量的 RT-PCR 被学习。Stretchstimulation 被发现 IGF-1 (L.IGF-1 和 MGF ) 的表示到职业人员警句,并且为两 isoformsexpression,更多被周期的段有效地比静态的段刺激。MGF 仅仅在受到机械段的造骨细胞被检测,建议 MGF 是段敏感生长因素。MGF 的表示比 L.IGF-1 的早达到顶点,它类似于他们在肌肉的规定并且作为在肌肉房间在骨头建议了 MGF 和 L.IGF-1 的类似的角色。在造骨细胞的 MGF 和 L.IGF-1 的功能将被进一步试验性的研究建立。 | XIAN Chengyu WANG Yuanliang ZHANG Bingbing TANG Liling PAN Jun LUO Yanfeng JIANG Peng LI Dajun | 2006 | Chinese Science Bulletin2006，51，22： | 7 |
| 15 | Effect of Ni Loading and Ce_(x)Zr_(1-x)O_(2) Promoter on Ni-Based SBA-15 Catalysts for Steam Reforming of Methane显示文摘有象 10wt%Ni/10wt%CexZr1_x:O2/SBA-15 一样从 5wt%到 20wt% 的镍内容的一系列 Ni/SBA-15 催化剂(x=0, 0.5, 1 ) 被准备。催化剂的结构用 XR.D, TPR, TEM 和赌注技术被描绘。为甲烷改过的蒸气的催化剂的催化活在连续流动被评估微反应堆。结果显示了那 Ni/SBA-15 和 Ni/CexZr1_x; O2/SBA-15 催化剂在气压有好催化活。10wt%Ni/SBA-15 催化剂在 800 点展出了优秀稳定性 ?? 在 740 h 的溪流上的时间的 6 号元素碳的化学符号。在反应以后,碳沉积没在催化剂的表面上被形成。在那里存在一常规六角形我在 Ni/SBA-15 和 Ni/CexZr1_x 的酣睡的结构; O2/SBA-15 催化剂。镍种类和 CexZr1_x; O2 部件都在 SBA-15 中央毛孔被限制。CexZr1_x; O2 能在 Ni/CexZr1_x 支持镍种类的分散; O2/SBA-15 催化剂。给词调音:基于 Ni 的催化剂;SBA-15;甲烷;冒热气改过; | Huijun Wan Xiujin Li Shengfu Ji Bingyao Huang Kai Wang Chengyue Li | 2007 | Journal of Natural Gas Chemistry2007，16，2： | 6 |
| 16 | Tauroursodeoxycholic acid(TUDCA) inhibits influenza A viral infection by disrupting viral proton channel M2显示文摘Influenza is a persistent threat to human health and there is a continuing requirement for updating antiinfluenza strategies. Initiated by observations of different endoplasmic reticulum(ER) responses of host to seasonal H1N1 and highly pathogenic avian influenza(HPAI) A H5N1 infections, we identified an alternative antiviral role of tauroursodeoxycholic acid(TUDCA), a clinically available ER stress inhibitor, both in vitro and in vivo. Rather than modulating ER stress in host cells, TUDCA abolished the proton conductivity of viral M2 by disrupting its oligomeric states, which induces inefficient viral infection. We also showed that M2 penetrated cells, whose intracellular uptake depended on its proton channel activity,an effect observed in both TUDCA and M2 inhibitor amantadine. The identification and application of TUDCA as an inhibitor of M2 proton channel will expand our understanding of IAV biology and complement current anti-IAV arsenals. | Ning Li Yanxu Zhang Shuangxiu Wu Ruodan Xu Zhiqing Li Jindong Zhu Hongliang Wang Xiao Li Mingyao Tian Huijun Lu Ningyi Jin Chengyu Jiang | 2019 | Science Bulletin2019，64，3： | 6 |
| 17 | Elevated plasma levels of selective cytokines in COVID-19 patients reflect viral load and lung injury显示文摘A recent outbreak of pneumonia in Wuhan,China was found to be caused by a 2019 novel coronavirus(2019-nCoV or SARS-CoV-2 or HCoV-19).We previously reported the clinical features of 12 patients with2019-n Co V infections in Shenzhen,China.To further understand the pathogenesis of COVID-19 and find better ways to monitor and treat the disease caused by 2019-n Co V,we measured the levels of 48 cytokines in the blood plasma of those 12 COVID-19 patients.Thirty-eight out of the 48 measured cytokines in the plasma of 2019-n Co V-infected patients were significantly elevated compared to healthy individuals.Seventeen cytokines were linked to 2019-nCoV loads.Fifteen cytokines,namely M-CSF,IL-10,IFN-α2,IL-17,IL-4,IP-10,IL-7,IL-1 ra,G-CSF,IL-12,IFN-γ,IL-1α,IL-2,HGF and PDGF-BB,were strongly associated with the lung-injury Murray score and could be used to predict the disease severity of2019-n Co V infections by calculating the area under the curve of the receiver-operating characteristics.Our results suggest that 2019-nCoV infections trigger extensive changes in a wide array of cytokines,some of which could be potential biomarkers of disease severity of 2019-n Co V infections.These findings will likely improve our understanding of the immunopathologic mechanisms of this emerging disease.Our results also suggest that modulators of cytokine responses may play a therapeutic role in combating the disease once the functions of these elevated cytokines have been characterized. | Yingxia Liu Cong Zhang Fengming Huang Yang Yang Fuxiang Wang Jing Yuan Zheng Zhang Yuhao Qin Xiaoyun Li Dandan Zhao Shunwang Li Shuguang Tan Zhaoqin Wang Jinxiu Li Chenguang Shen Jianming Li Ling Peng Weibo Wu Mengli Cao Li Xing Zhixiang Xu Li Chen Congzhao Zhou William J Liu Lei Liu Chengyu Jiang | 2020 | National Science Review2020，7，6： | 6 |
| 18 | Nanocrystalline TiO_(2) thin film electrodes prepared by common pressure hydrothermal method at low temperature显示文摘Nanocrystalline TiO2 thin film electrodes have been prepared from mixed pastes of tetrabutyl titanate and nanocrystalline TiO2 particles by common pressure hydro- thermal method at low temperature. The tetrabutyl titanate was hydrolyzed and crystallized into anatase TiO2 to inter- connect nanocrystalline TiO2 particles and adhere them to conductive substrates, obtaining highly porous and me- chanically stable TiO2 nanocrystalline film. The conversion efficiencies of the dye-sensitized solar cells based on prepared electrodes on conductive glass substrates and flexible sub- strates were 4.8% and 1.9% under illumination of 100 mW/cm2, respectively. | LI Chengyu LIN Yuan LI Xueping WANG Zhengping MA Yutao ZHOU Xiaowen FENG Shujing XIAO Xurui | 2005 | Chinese Science Bulletin2005，50，14： | 5 |
| 19 | Defect in Brnym1,a magnesium-dechelatase protein,causes a stay-green phenotype in an EMSmutagenized Chinese cabbage(Brassica campestris L.ssp.pekinensis)line显示文摘Leaf color is an important target trait in Chinese cabbage breeding programs.Leaf yellowing may reduce crop commercial and nutritional values.Some plants with the“stay-green”trait maintain leaf greenness during senescence and even after death.Stay-green Chinese cabbage may be a focal point of future breeding projects because it could improve crop quality and yield and prolong shelf life.A new stay-green mutant,non-yellowing mutant 1(nym1),was identified in Chinese cabbage derived from an ethyl methane sulfonate(EMS)-mutagenized population.The mutant had stay-green characteristics and a higher chlorophyll content than the wild-type during leaf senescence.The staygreen trait in the mutant Chinese cabbage was controlled by the recessive gene Brnym1.MutMap and KASP analyses showed that Brnym1(BraA03g050600.3C)encodes an mg-dechelatase(SGR protein),which might be the causal gene of the mutation in Chinese cabbage.A nonsynonymous single nucleotide base substitution(G to A)in the third exon of Brnym1 caused an amino acid substitution from L to F in the highly conserved domain of the magnesiumdechelatase.Ectopic overexpression showed that the BrNYM1 gene of wild-type Chinese cabbage complemented the SGR-defective stay-green mutant nye1-1 of Arabidopsis.The magnesium-dechelatase activity in the nym1 mutant was significantly downregulated compared to that in the wild type.Brnym1 was relatively upregulated in the mutant during late senescence,and BrNYM1 was localized to the chloroplasts.These results indicate that Brnym1(BraA03g050600.3C)is the causal gene of the stay-green mutation and could be of particular significance in the genetic improvement of Chinese cabbage. | Nan Wang Yun Zhang Shengnan Huang Zhiyong Liu Chengyu Li Hui Feng | 2020 | Horticulture Research2020，7，1： | 5 |
| 20 | Human RBCs blood group conversion from A to O using a novel α-N-acetylgalactosaminidase of high specific activity显示文摘α-N-acetylgalactosaminidase (αNAGA) can convert group A human red blood cells (RBCs) to group O. One novel αNAGA gene was cloned by PCR from Elizabethkingia meningosepticum isolated from a domestic clinical sample. Pure recombinant αNAGA was obtained by genetic engineering and protein purification with a calculated molecule of 49.6 kD. αNAGA was selective for terminal α-N-acetylgalacto-samine residue with a high specific activity. αNAGA could completely remove A antigens of 1 U (about 100 mL) group A1 or A2 RBCs in 1 h at pH 6.8 and 25℃ with a consumption of 1.5 or 0.4 mg recombinant enzyme. Enzyme-converted group A RBCs did not agglutinate after being mixed with monoclonal anti-A or sera of groups A,B,AB and O. Other blood group antigens except ABO had no change. FCM analy-sis showed that A antigens and A1 antigens disappeared while H antigens increased. It indicated that αNAGA successfully converted human blood group A RBCs to universally transfusable group O RBCs without the risk of ABO-incompatible transfusion reactions. This αNAGA was suitable for producing universal RBCs to increase clinical transfusion safety,improve the RBCs supply,and to decrease transfusion cost and support transfusion service in case of emergency. | YU ChengYu XU Hua WANG LiSheng ZHANG JianGeng ZHANG YangPei | 2008 | Chinese Science Bulletin2008，53，13： | 5 |
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