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| 1 | clusterProfiler 4.0:A universal enrichment tool for interpreting omics data显示文摘Functional enrichment analysis is pivotal for interpreting highthroughput omics data in life science.It is crucial for this type of tool to use the latest annotation databases for as many organisms as possible.To meet these requirements,we present here an updated version of our popular Bioconductor package,clusterProfiler 4.0.This package has been enhanced considerably compared with its original version published 9 years ago.The new version provides a universal interface for functional enrichment analysis in thousands of organisms based on internally supported ontologies and pathways as well as annotation data provided by users or derived from online databases.It also extends the dplyr and ggplot2 packages to offer tidy interfaces for data operation and visualization.Other new features include gene set enrichment analysis and comparison of enrichment results from multiple gene lists.We anticipate that clusterProfiler 4.0 will be applied to a wide range of scenarios across diverse organisms. | Tianzhi Wu Erqiang Hu Shuangbin Xu Meijun Chen Pingfan Guo Zehan Dai Tingze Feng Lang Zhou Wenli Tang Li Zhan Xiaocong Fu Shanshan Liu Xiaochen Bo Guangchuang Yu | 2021 | The Innovation2021，2，3： | 40 |
| 2 | Exotic origin of the Chinese continental shelf: new insights into the tectonic evolution of the western Pacific and eastern China since the Mesozoic显示文摘The effect of paleo-Pacific subduction on the geological evolution of the western Pacific and continental China is likely complex. Nevertheless, our analysis of the distribution of Mesozoic granitoids in the eastern continental China in space and time has led us to an interesting conclusion: The basement of the continental shelf beneath East and South China Seas may actually be of exotic origin geologically unrelated to the continental lithosphere of eastern China. By accepting the notion that the Jurassic–Cretaceous granitoids in the region are genetically associated with western Pacific subduction and the concept that subduction may cease to continue only if the trench is being jammed, then the termination of the granitoidmagmatism throughout the vast region at *88 ± 2 Ma manifests the likelihood of ‘‘sudden'', or shortly beforehand(*100 Ma), trench jam of the Mesozoic western Pacific subduction. Trench jam happens if the incoming‘‘plate'' or portion of the plate contains a sizeable mass that is too buoyant to subduct. The best candidate for such a buoyant and unsubductable mass is either an oceanic plateau or a micro-continent. We hypothesize that the basement of the Chinese continental shelf represents such an exotic, buoyant and unsubductable mass, rather than seaward extension of the continental lithosphere of eastern China. The locus of the jammed trench(i.e., the suture) is predictably located on the shelf in the vicinity of, and parallel to, the arc-curved coastal line of the southeast continental China. It is not straightforward to locate the locus in the northern section of the East China Sea shelf because of the more recent(\20 Ma) tectonic re-organization associated with the opening of the Sea of Japan. We predict that the trench jam at *100 Ma led to the re-orientation of the Pacific plate motion in the course of NNW direction as inferred from the age-progressive Emperor Seamount Chain of Hawaiian hotspot origin(its oldest unsubdued Meiji and Detroit seamounts are *82 Ma),making the boundary between the Pacific plate and the newly accreted plate of eastern Asia a transform fault at the location east of the continental shelf of exotic origin. This explains the apparent *40 Myr magmatic gap from *88to *50 Ma prior to present-day western Pacific subduction initiation. We propose that basement penetration drilling on well-chosen sites is needed to test the hypothesis in order to reveal the true nature of the Chinese continental shelf basement. This testing becomes critical and cannot longer be neglected in order to genuinely understand the tectonic evolution of the western Pacific and its effect on the geology of eastern China since the Mesozoic, includingthe cratonic lithosphere thinning, related magmatism/mineralization, and the mechanism of the subsequent South China Sea opening, while also offering novel perspectives on aspects of the plate tectonics theory. We also suggest the importance of future plate tectonic reconstruction of the western Pacific to consider the nature and histories of the Chinese continental shelf of exotic origin as well as the probable transform plate boundary from *100 to*50 Ma. Effort is needed to reveal the true nature and origin of the *88 ± 2 Ma granitic gneisses in Taiwan and the 110–88 Ma granitoids on the Hainan Island. | Yaoling Niu Yi Liu Qiqi Xue Fengli Shao Shuo Chen Meng Duan Pengyuan Guo Hongmei Gong Yan Hu Zhenxing Hu Juanjuan Kong Jiyong Li Jinju Liu Pu Sun Wenli Sun Lei Ye Yuanyuan Xiao Yu Zhang | 2015 | Science Bulletin2015，60，18： | 32 |
| 3 | Climatic change features of fog and haze in winter over North China and Huang-Huai Area显示文摘This paper revealed the climatic change characteristics of fog and haze of different levels over North China and Huang-Huai area(NCHH).It was found that the haze-prone period has changed from winter into a whole year,and the haze days(HD)in winter have increased significantly.The foggy days(FD)are half of HD.There are little difference on the number of days and trends of fog at various levels.The HD and FD show no obvious positive correlation until the 1980s.Fog has larger spatial scale,showing more in the south than in the north.Haze occurs mainly around large cities with a discrete distribution.In the background of weakened East Asian Winter Monsoon(EAWM)and sufficient particulate matter,the negative correlation between haze and wind speed is weakened,but the positive correlation between haze and moisture conditions(precipitation and humidity)is significantly strengthened.In recent years,small wind and variability appear frequently.Meanwhile,as the stable source and strong moisture absorption of the aerosol particles,the moisture condition becomes one key control factor in the haze,especially wet haze with less visibility.In contrast,the FD presents a stable positive correlation with precipitation and relative humidity,but has no obvious negative correlation with wind speed. | YIN ZhiCong WANG HuiJun GUO WenLi | 2015 | Science China Earth Sciences2015，58，8： | 19 |
| 4 | Wheat breeding in northern China: Achievements and technical advances显示文摘Common wheat is the major cereal crop that underpins the food safety of China. Both winter wheat and spring wheat are grown on ~24 million ha. This review aims to summarize the current status of wheat production and breeding progress in the northern wheat production areas of the country, and to review recently advanced technologies being applied in wheat breeding, including the use of dwarf-male-sterile(DMS) wheat, speed breeding and specialized wheat breeding SNP chips. Crossing is the initial step in most breeding programs. DMS wheat is a convenient tool for large scale production of hybrid seed. Speed breeding or accelerated generation turnover attempts to reduce the time taken in cultivar development. Several different SNP chips are high-throughput, genome-wide genotyping platforms for breeding and research. | Hongjie Li Yang Zhou Wenli Xin Yiqin Wei Junling Zhang Lilei Guo | 2019 | The Crop Journal2019，7，6： | 16 |
| 5 | An important role of the hepcidin-ferroportin signaling in affecting tumor growth and metastasis显示文摘 | Wenli Guo Shuping Zhang Yue Chen Daoqiang Zhang Lin Yuan Haibo Cong Sijin Liu | 2015 | Acta Biochimica et Biophysica Sinica2015，47，9： | 14 |
| 6 | Genetic and biological characteristics of the globally circulating H5N8 avian influenza viruses and the protective efficacy offered by the poultry vaccine currently used in China显示文摘The H5N8 avian influenza viruses have been widely circulating in wild birds and are responsible for the loss of over 33 million domestic poultry in Europe, Russia, Middle East, and Asia since January 2020. To monitor the invasion and spread of the H5N8 virus in China, we performed active surveillance by analyzing 317 wild bird samples and swab samples collected from 41,172 poultry all over the country. We isolated 22 H5N8 viruses from wild birds and 14 H5N8 viruses from waterfowls. Genetic analysis indicated that the 36 viruses formed two different genotypes: one genotype viruses were widely detected from different wild birds and domestic waterfowls;the other genotype was isolated from a whopper swan. We further revealed the origin and spatiotemporal spread of these two distinct H5N8 virus genotypes in 2020 and 2021. Animal studies indicated that the H5N8 isolates are highly pathogenic to chickens, mildly pathogenic in ducks, but have distinct pathotypes in mice. Moreover, we found that vaccinated poultry in China could be completely protected against H5N8 virus challenge. Given that the H5N8 viruses are likely to continue to spread in wild birds, vaccination of poultry is highly recommended in high-risk countries to prevent H5N8 avian influenza. | Pengfei Cui Xianying Zeng Xuyong Li Yanbing Li Jianzhong Shi Conghui Zhao Zhiyuan Qu Yanwen Wang Jing Guo Wenli Gu Qi Ma Yuancheng Zhang Weipeng Lin Minghui Li Jingman Tian Dongxue Wang Xin Xing Yanjing Liu Shuxin Pan Yaping Zhang Hongmei Bao Liling Liu Guobin Tian Chengjun Li Guohua Deng Hualan Chen | 2022 | Science China(Life Sciences)2022，65，4： | 12 |
| 7 | Research and application on the technology system of multi-scale assessment of the impact on the atmospheric environment by urban planning显示文摘Improvement of the atmospheric environment in urban planning is an important issue to implement the sustainable development of cities. In this paper, in order to meet the demand of planning office to compare and assess quantitatively of the designs in multi-scale, based on geographical information system (GIS) data with high resolution, a multi-scale numerical modeling system for the atmospheric environment impact of urban planning is set up, and the multi-scale assessment index system is established, which compose the technology system of multi-scale assessment of the impact on the atmospheric environment by urban planning. In urban planning (urban development of Beijing) and optimizing layouts of Olympic Green, it has been applied to quantitatively evaluating the impact on atmospheric environment by urban planning before construction, which offers scientific foundation to optimize the whole and local urban layout. | WANG Guangtao, WANG Xiaoyun, MIAO Shiguang, JIANG Weimei, GUO Wenli, JI Chongping & CHEN Xianyan School of Architecture, Tsinghua University, Beijing 100084, China Ministry of Construction of China, Beijing 100044, China Beijing Meteorological Bureau, Beijing 100089, China Institute of Urban Meteorology, China Meteorological Administration, Beijing 100089, China Department of Atmospheric Sciences, Nanjing University, Nanjing 210093, China | 2005 | Science China Earth Sciences2005，48，z2： | 11 |
| 8 | A Linear-correction Least-squares Approach for Geolocation Using FDOA Measurements Only显示文摘A linear-correction least-squares(LCLS) estimation procedure is proposed for geolocation using frequency difference of arrival (FDOA) measurements only. We first analyze the measurements of FDOA, and further derive the Cramér-Rao lower bound (CRLB) of geolocation using FDOA measurements. For the localization model is a nonlinear least squares(LS) estimator with a nonlinear constrained, a linearizing method is used to convert the model to a linear least squares estimator with a nonlinear constrained. The Gauss-Newton iteration method is developed to conquer the source localization problem. From the analysis of solving Lagrange multiplier, the algorithm is a generalization of linear-correction least squares estimation procedure under the condition of geolocation using FDOA measurements only. The algorithm is compared with common least squares estimation. Comparisons of their estimation accuracy and the CRLB are made, and the proposed method attains the CRLB. Simulation results are included to corroborate the theoretical development. | LI Jinzhou GUO Fucheng JIANG Wenli | 2012 | Chinese Journal of Aeronautics2012，25，5： | 10 |
| 9 | First light of the 1.8-m solar telescope–CLST显示文摘Large-aperture solar telescopes play an important role in solar observations and research,and require high temporal and spatial resolution[1].To solve some fundamental problems such as the solar dynamo,coronal heating,and the triggering of major solar eruptions,the spatial resolution for solar-atmosphere observation should reach at least 0.1 arcsec[2]. | ChangHui Rao NaiTing Gu XueJun Rao Cheng Li LanQiang Zhang JinLong Huang Lin Kong Ming Zhang YunTao Cheng Yi Pu Hua Bao YouMing Guo YangYi Liu JinSheng Yang LiBo Zhong ChangJun Wang Kai Fang XiaoJun Zhang DongHong Chen Cheng Wang XinLong Fan ZhiWu Yan KeLe Chen XiYa Wei Lei Zhu Hong Liu YongJian Wan Hao Xian WenLi Ma | 2020 | Science China(Physics,Mechanics & Astronomy)2020，63，10： | 6 |
| 10 | Emodin prevents hypoxic-ischemic neuronal injury Involvement of the activin A pathway显示文摘Emodin, an extract of dried rhizomes and the root of the Rhizoma Polygoni Cuspidati, can protect neurons from hypoxic-ischemic brain damage. This study aimed to verify the underlying mechanism. After PC12 cells had differentiated into neuron-like cells under the induction of mouse nerve growth factor, cells were subjected to oxygen-glucose deprivation and treated with emodin. Results showed that the viability of neuron-like cells cultured under an ischemia-hypoxia environment decreased, while the expression of activin A and caspase-3 in cells increased. Emodin raised the survival rate of oxygen-glucose deprived neuron-like cells, increased activin A expression, and decreased caspase-3 expression. Experimental findings indicate that emodin can inhibit neuronal apoptosis and alleviate the injury of nerve cells after oxygen-glucose deprivation through the activin A pathway. | Hongliang Guo Xiaoran Shen Ye Xu Junliang Yuan Dongming Zhao Wenli Hu | 2013 | Neural Regeneration Research2013，8，15： | 5 |
| 11 | Source localization and calibration using TDOA and FDOA measurements in the presence of sensor location uncertainty显示文摘Source localization accuracy is very sensitive to sensor location error.This paper performs analysis and develops a solution for locating a moving source using time difference of arrival(TDOA)and frequency difference of arrival(FDOA)measurements with the use of a calibration emitter.Using a Gaussian random signal model,we first derive the Cram′er-Rao lower bound(CRLB)for source location estimate in this scenario.Then we analyze the differential calibration technique which is commonly used in Global Positioning System.It is indicated that the differential calibration cannot attain the CRLB accuracy in most cases.A closed-form solution is then proposed which takes a calibration emitter into account to reduce sensor location error.It is shown analytically that under some mild approximations,our approach is able to reach the CRLB accuracy.Numerical simulations are included to corroborate the theoretical developments. | LI JinZhou GUO FuCheng JIANG WenLi | 2014 | Science China(Information Sciences)2014，57，4： | 4 |
| 12 | Design and application of 60mer oligonucleotide microarray in SARS coronavirus detection显示文摘The 60mer oligonucleotide microarray was designed and applied to detecting of SARS (severe acute res-piratory syndrome) coronavirus. Thirty 60mer specific oligos were designed to cover the whole genome of the first submit-ted coronavirus strain, according to the sequence of TOR2 (GENEBANK Accession: AY274119). These primers were synthesized and printed into a microarray with 12×12 spots. RNAs were extracted from the throat swab and gargling fluid of SARS patients and reverse-transcripted into the double strand cDNAs. The cDNAs were prepared as re-stricted cDNA fragments by the restriction display (RD) technique and labeled by PCR with the Cy5-universal primer. The labeled samples were then applied to the oligo microar-ray for hybridization. The diagnostic capability of the mi-croarray was evaluated after the washing and scanning steps. The scanning result showed that samples of SARS patients were hybridized with multiple SARS probes on the microar-ray, and there is no signal on the negative and blank controls. These results indicate that the genome of SARS coronavirus can be detected in parallel by the 60mer oligonucleotide mi-croarray, which can improve the positive ratio of the diagno-sis. The oligo microarray can also be used for monitoring the behavior of the virus genes in different stages of the disease status. | SHI Rong MA Wenli WU Qinghua ZHANG Bao SONG Yanbin GUO Qiuye XIAO Weiwei WANG Yan ZHENG Wenling | 2003 | Chinese Science Bulletin2003，48，12： | 4 |
| 13 | Coprinus comatus-derived nitrogen-containing biocarbon electrocatalyst with the addition of self-generating graphene-like support for superior oxygen reduction reaction显示文摘The development of nitrogen-rich biomassderived carbon catalysts provides an attractive perspective to substitute for Pt-based electrocatalysts for oxygen reduction reaction(ORR). We here report a facile strategy for synthesis of a nitrogen-doped biocarbon/graphene-like composite electrocatalyst by pyrolyzing a solid-state mixture of coprinus comatus biomass and melamine under nitrogen protection. The graphtic carbon nitride formed by polycondensation of melamine at 600 °C acts as a selfsacrificing template to generate the nitrogen-doped graphene-like sheet, which can function as an inserting agent and self-generating support. The composite catalyst exhibits the most promising catalytic activity towards the fourelectron ORR with a half-wave potential of around 0.83 V(vs. RHE), and more excellent stability and tolerance to methanol/ethanol compared to the commercial Pt/C catalyst. It is interestingly found that both a higher content of nitrogen and a larger ratio of graphitic-nitrogen species,which may derive from self-addition of graphene-like support into the catalyst, can effectively improve the electrocatalytic activity. The planar N group may be the nitrogen functionality that is most responsible for maintaining the ORR activity in alkaline medium. This study can largely encourage the exploration of high-performance carbon-based catalysts from economical and sustainable fungus biomass. | Chaozhong Guo Wenli Liao Zhongbin Li Lingtao Sun Haibo Ruan Qingshan Wu Qinghong Luo Jie Huang Changguo Chen | 2016 | Science Bulletin2016，61，12： | 4 |
| 14 | Disruption of iron homeostasis and resultant health effects upon exposure to various environmental pollutants:A critical review显示文摘Environmental pollution has become one of the greatest problems in the world, and the concerns about environmental pollutants released by human activities from agriculture and industrial production have been continuously increasing. Although intense efforts have been made to understand the health effects of environmental pollutants, most studies have only focused on direct toxic effects and failed to simultaneously evaluate the long-term adaptive, compensatory and secondary impacts on health. Burgeoning evidence suggests that environmental pollutants may directly or indirectly give rise to disordered element homeostasis, such as for iron. It is crucially important to maintain concerted cellular and systemic iron metabolism. Otherwise, disordered iron metabolism would lead to cytotoxicity and increased risk for various diseases, including cancers. Thus, study on the effects of environmental pollutants upon iron homeostasis is urgently needed. In this review, we recapitulate the available findings on the direct or indirect impacts of environmental pollutants, including persistent organic pollutants(POPs), heavy metals and pesticides, on iron homeostasis and associated adverse health problems. In view of the unanswered questions, more efforts are warranted to investigate the disruptive effects of environmental pollutants on iron homeostasis and consequent toxicities. | Wenli Guo Jie Zhang Wenjun Li Ming Xu Sijin Liu | 2015 | Journal of Environmental Sciences2015，27，8： | 3 |
| 15 | Computation of an Emptiable Minimal Siphon in a Subclass of Petri Nets Using Mixed-Integer Programming显示文摘Deadlock resolution strategies based on siphon control are widely investigated.Their computational efficiency largely depends on siphon computation.Mixed-integer programming(MIP)can be utilized for the computation of an emptiable siphon in a Petri net(PN).Based on it,deadlock resolution strategies can be designed without requiring complete siphon enumeration that has exponential complexity.Due to this reason,various MIP methods are proposed for various subclasses of PNs.This work proposes an innovative MIP method to compute an emptiable minimal siphon(EMS)for a subclass of PNs named S^(4)PR.In particular,many particular structural characteristics of EMS in S4 PR are formalized as constraints,which greatly reduces the solution space.Experimental results show that the proposed MIP method has higher computational efficiency.Furthermore,the proposed method allows one to determine the liveness of an ordinary S^(4)PR. | Shouguang Wang Wenli Duo Xin Guo Xiaoning Jiang Dan You Kamel Barkaoui MengChu Zhou | 2021 | IEEE/CAA Journal of Automatica Sinica2021，8，1： | 2 |
| 16 | Gauss optimization method for the dynamics of unilateral contact of rigid multibody systems显示文摘The discontinuous dynamical problem of multi-point contact and collision in multi-body system has always been a hot and difficult issue in this field.Based on the Gauss’principle of least constraint,a unified optimization model for multibody system dynamics with multi-point contact and collision is established.The paper presents the study of the numerical solution scheme,in which particle swarm optimization method is used to deal with the corresponding optimization model.The article also presents the comparison of the Gauss optimization method(GOM)and the hybrid linear complementarity method(i.e.combining differential algebraic equations(DAEs)and linear complementarity problems(LCP)),commonly used to solve the dynamic contact problem of multibody systems with bilateral constraints.The results illustrate that,the GOM has the same advantage of dynamical modelling with LCP and when the redundant constraint exists,the GOM always has a unique solution and so no additional processing is needed,whereas the corresponding DAE-LCP method may have singular cases with multiple solutions or no solutions.Using numerical examples,the GOM is verified to effectively solve the dynamics of multibody systems with redundant unilateral and bilateral constraints without additional redundancy processing.The GOM can also be applied to the optimal control of systems in the future and combined with the parameter optimization of systems to handle dynamic problems.The work given provides the dynamics and control of the complex system with a new train of thought and method. | Wenli Yao Liusong Yang Mingming Guo | 2021 | Acta Mechanica Sinica2021，37，3： | 2 |
| 17 | Dispersion Performance of Methanol-Diesel Emulsified Fuel Prepared by High Gravity Technology显示文摘A new continuous process for preparing methanol-diesel emulsified fuel with an Impinging Stream-Rotating Packed Bed is proposed. The droplet size of dispersed phase(methanol) of the emulsified fuel has a significant effect on the combustion of methanol-diesel emulsified fuel. In this paper, the methanol-diesel emulsified fuel uses diesel as the continuous phase and methanol as the dispersed phase. The Sauter mean diameter of the dispersed phase of methanol-diesel emulsified fuel was characterized with microphotography and arithmetic method. The experimental result showed that the Sauter mean diameter of the dispersed phase, which was decreased with the augmentation of the high gravity factor, liquid flow rate and emulsifier dosage, was inversely proportional to the methanol content. The Sauter mean diameter of the dispersed phase can be controlled and adjusted in the range of 12—40 μm through the change of operating conditions. The correlative expressions of the Sauter mean diameter of emulsified fuel were obtained and the calculated values agreed well with the experimental values. | Jiao Weizhou Li Jing Liu Youzhi Zhang Qiaoling Liu Wenli Xu Chengcheng Guo Liang | 2014 | China Petroleum Processing & Petrochemical Technology2014，16，1： | 2 |
| 18 | In situ growth of NiS_(2) nanosheet array on Ni foil as cathode to improve the performance of lithium/sodium-sulfur batteries显示文摘The NiS;nanosheet array on Ni foil(NiS2/NF)was prepared using an in situ growth strategy and sulfidation method and was used as the cathode of lithium sulfur battery.The unique nanostructure of the NiS;nanosheet array can provide abundant active sites for the adsorption and chemical action of polysulfides.Compared with the sulfur powder coated pure NF(pure NF-S)for lithium sulfur battery,the sulfur powder coated NiS_(2)/NF(NiS_(2)/NF-S)electrode exhibits superior electrochemical performance.Specifically,the NiS_(2)/NF-S delivered a high reversible capacity of 1007.5 m Ah g^(-1) at a current density of 0.1 C(1 C=1675 mA g^(-1))and kept 74.5% of the initial capacity at 1.0 C after 200 cycles,indicating the great promise of NiS_(2)/NF-S as the cathode of lithium sulfur battery.In addition,the NiS_(2)/NF-S electrode also showed satisfactory electrochemical performance when used as the cathode for sodium sulfur battery. | FAN MouPing CHEN YuanMao KE Xi HUANG ZeXi CHEN YouChen WU WenLi QU XiaoFeng SHI ZhiCong GUO ZaiPing | 2022 | Science China(Technological Sciences)2022，65，1： | 2 |
| 19 | Easy domain adaptation method for filling the species gap in deep learning-based fruit detection显示文摘Fruit detection and counting are essential tasks for horticulture research.With computer vision technology development,fruit detection techniques based on deep learning have been widely used in modern orchards.However,most deep learning-based fruit detection models are generated based on fully supervised approaches,which means a model trained with one domain species may not be transferred to another.There is always a need to recreate and label the relevant training dataset,but such a procedure is time-consuming and labor-intensive.This paper proposed a domain adaptation method that can transfer an existing model trained from one domain to a new domain without extra manual labeling.The method includes three main steps:transform the source fruit image(with labeled information)into the target fruit image(without labeled information)through the CycleGAN network;Automatically label the target fruit image by a pseudo-label process;Improve the labeling accuracy by a pseudo-label self-learning approach.Use a labeled orange image dataset as the source domain,unlabeled apple and tomato image dataset as the target domain,the performance of the proposed method from the perspective of fruit detection has been evaluated.Without manual labeling for target domain image,the mean average precision reached 87.5%for apple detection and 76.9%for tomato detection,which shows that the proposed method can potentially fill the species gap in deep learning-based fruit detection. | Wenli Zhang Kaizhen Chen Jiaqi Wang Yun Shi Wei Guo | 2021 | Horticulture Research2021，8，1： | 2 |
| 20 | Transient receptor potential vanilloid 1-based gene therapy alleviates orthodontic pain in rats显示文摘Orthodontic pain that is induced by tooth movement is an important sequela of orthodontic treatment and has a significant effect on patient quality of life. Studies have shown that the high expression of transient receptor potential vanilloid 1(TRPV1) in trigeminal ganglions plays a vital role in the transmission and modulation of orofacial pain. However, little is known about the role of TRPV1 in orthodontic pain. In this study, male Sprague–Dawley rats were randomly assigned to six groups to study the role of TRPV1 in the modulation of tooth-movement pain. The expression levels of TRPV1 mRNA and protein were determined by real-time PCR and western blot, respectively. Moreover, pain levels were assessed using the rat grimace scale(RGS). The role of TRPV1 in modulating tooth-movement pain was examined by injecting a TRPV1 antagonist into the trigeminal ganglia of rats. A lentivirus containing a TRPV1 shRNA sequence was constructed and transduced into the rats' trigeminal ganglia. The results showed that the expression levels of TRPV1 protein and m RNA were elevated following tooth-movement pain. Pain levels increased rapidly on the1 stday, peaked on the 3^(rd) day and returned to baseline on the 14 thday. The TRPV1 antagonist significantly reduced toothmovement pain. The lentivirus containing a TRPV1 shRNA sequence was able to inhibit the expression of TRPV1 and relieved toothmovement pain. In conclusion, TRPV1-based gene therapy may be a treatment strategy for the relief of orthodontic pain. | Rui Guo Yang Zhou Hu Long Di Shan Jing Wen Huimin Hu Hong Yang Zhouqiang Wu Wenli Lai | 2019 | International Journal of Oral Science2019，11，1： | 2 |
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