|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Reprogramming of ovine adult fibroblasts to pluripotency via drug-inducible expression of defined factors显示文摘在 enucleated 鸡蛋的体的房间的 Reprogramming 做了多丽,绵羊,在 1996 的第一个成功地克隆的哺乳动物。然而,绵羊的机制体的房间 reprogramming 还没被探讨了。而且,绵羊胚胎的茎(ES ) 细胞仍然不是可得到的,它限制精确修改基因的绵羊的产生。在这研究,我们报导体的房间能是的那只绵羊直接 reprogrammed 到导致的 pluripotent 用定义因素(Oct4, Sox2, c-Myc, Klf4, Nanog, Lin28, SV40 大 T 和 hTERT ) 的茎(iPS ) 房间。我们的观察显示从绵羊的体的细胞比从另外的种,在 iPS,细胞被报导了的体的细胞对改编更困难。我们证明绵羊 iPS 房间表示 ES 房间标记,包括碱的磷酸酶, Oct4, Nanog, Sox2, Rex1,阶段特定的胚胎的 antigen-1, TRA-1-60, TRA-1-81 和 E-cadherin。绵羊 iPS 房间展出了正常 karyotypes 并且能在 vitro 并且在 teratomas 区分进所有三细菌层。我们的学习可以帮助在绵羊揭示体的房间 reprogramming 的机制并且提供一个平台为绵羊 ES 房间探索文化条件。而且,绵羊 iPS 房间可以直接被用来产生精确修改基因的绵羊。 | Lei Bao Lixiazi He Jijun Chen Zhao Wu Jing Liao Lmgdun Rao Jiangtao Ren Hui Li Hui Zhu Lei Qian Yijun Gu Huimin Dai Xun Xu Jinqiu Zhou Wen Wang Chun Cui Lei Xiao | 2011 | Cell Research2011，21，4： | 32 |
| 2 | Altered trends in carbon uptake in China’s terrestrial ecosystems under the enhanced summer monsoon and warming hiatus显示文摘The carbon budgets in terrestrial ecosystems in China are strongly coupled with climate changes.Over the past decade,China has experienced dramatic climate changes characterized by enhanced summer monsoon and decelerated warming.However,the changes in the trends of terrestrial net ecosystem production(NEP)in China under climate changes are not well documented.Here,we used three ecosystem models to simulate the spatiotemporal variations in China's NEP during 1982–2010 and quantify the contribution of the strengthened summer monsoon and warming hiatus to the NEP variations in four distinct climatic regions of the country.Our results revealed a decadal-scale shift in NEP from a downtrend of–5.95 Tg C/yr^2(reduced sink)during 1982–2000 to an uptrend of 14.22 Tg C/yr^2(enhanced sink)during 2000–10.This shift was essentially induced by the strengthened summer monsoon,which stimulated carbon uptake,and the warming hiatus,which lessened the decrease in the NEP trend.Compared to the contribution of 56.3%by the climate effect,atmospheric CO2 concentration and nitrogen deposition had relatively small contributions(8.6 and 11.3%,respectively)to the shift.In conclusion,within the context of the global-warming hiatus,the strengthening of the summer monsoon is a critical climate factor that enhances carbon uptake in China due to the asymmetric response of photosynthesis and respiration.Our study not only revealed the shift in ecosystem carbon sequestration in China in recent decades,but also provides some insight for understanding ecosystem carbon dynamics in other monsoonal areas. | Honglin He Shaoqiang Wang Li Zhang Junbang Wang Xiaoli Ren Lei Zhou Shilong Piao Hao Yan Weimin Ju Fengxue Gu Shiyong Yu Yuanhe Yang Miaomiao Wang Zhongen Niu Rcmg Ge Huimin Yan Mei Huang Guoyi Zhou Yongfei Bai Zongqiang Xie Zhiyao Tang Bingfang Wu Leiming Zhang Nianpeng He Qiufeng Wang Guirui Yu | 2019 | National Science Review2019，6，3： | 24 |
| 3 | Fabrication technique of micro/nano-scale speckle patterns with focused ion beam显示文摘The fabrication technique of micro/nano-scale speckle patterns with focused ion beam (FIB) system is studied for digital image correlation (DIC) measurement under a scanning electron microscope (SEM).The speckle patterns are fabricated by directly etching the counterpart of the specimen to the black part of a template.Mean intensity gradient is used to evaluate the quality of these SEM images of speckle patterns fabricated based on different templates to select an optimum template.The pattern size depending on the displacement measurement sensitivity is adjusted by altering the magnification of FIB according to the relation curve of the etching size versus magnification.The influencing factors including etching time and ion beam current are discussed.Rigid body translation tests and rotation tests are carried out under SEM to verify the reliability of the fabricated speckle patterns.The calculated values are in good agreement with the imposed ones. | LI YanJie XIE HuiMin LUO Qiang GU ChangZhi HU ZhenXing CHEN PengWan ZHANG QingMing | 2012 | Science China(Physics,Mechanics & Astronomy)2012，55，6： | 7 |
| 4 | Efficacy and Economic Evaluation of Nonbiological Artificial Liver Therapy in Acute-on-chronic Hepatitis B Liver Failure显示文摘Background and Aims:Nonbiological artificial liver(NBAL)is frequently used as a first-line treatment for hepatitis B virus-associated acute-on-chronic liver failure(HBV-ACLF).This study aimed to compare the therapeutic efficacy and cost-effectiveness ratio(CER)of comprehensive medical treatment,plasma exchange(PE),and double plasma molecular adsorption system(DPMAS)plus half-dose PE(DPMAS+PE)in patients with HBV-ACLF.Methods:A total of 186 patients with HBV-ACLF randomly received comprehensive medical treatment,PE,or DPMAS+PE and were prospectively evaluated.Patients were divided into four subgroups based on the pretreatment prothrombin activity(PTA):Group I(PTA>40%),group II(PTA 30–40%),group III(PTA 20–30%),and group IV(PTA<20%).The main outcome measures were 28 day effectiveness;90 day liver transplantation-free survival;change of biochemical parameters;and CER.Results:DPMAS+PE treatment was associated with significantly higher 28 day effectiveness and 90 day liver transplantation-free survival compared with PE treatment in patients with group I liver failure.Clearance of serum total bilirubin(TBIL),AST,and creatinine(Cr)were significantly higher in the DPMAS+PE group than in the PE group.For subjects with group I liver failure,DPMAS+PE treatment had advantages of lower CER values and better cost-effectiveness.Conclusions:Compared with comprehensive medical treatment and PE alone,DPMAS with halfdose sequential PE treatment more effectively improved TBIL,AST,and Cr in HBV-ACLF patients,improved 28 day effectiveness and 90 day survival rates in patients with group I liver failure,and was more cost effective.DPMAS+PE is a viable NBAL approach for treatment of HBV-ACLF. | Cichun Wu Wenting Peng Da Cheng Huimin Gu Fei Liu Shifang Peng Lei Fu | 2023 | Journal of Clinical and Translational Hepatology2023，11，2： | 5 |
| 5 | Preparation of Monodisperse Spherical SiO_2 by Microwave Hydrothermal Method and Kinetics of Dehydrated Hydroxyl显示文摘Monodisperse spherical SiO2 particles were successfully synthesized in 2-propanol-H2O-NH3 system by the microwave hydrothermal method using ammonia as catalyst. To investigate the influences on the size of spherical SiO2 particles, factors such as ammonia concentration, reaction temperature, stirring intensity and reactants mol ratio have been studied. The orthogonal experiments were carried out. The as-prepared SiO2 particles were characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR) and differential thermal analysis (DTA). The results indicated that the size of SiO2 particles increased greatly with the increase in ammonia concentration, temperature and reactants mol ratio, but increased slightly with the increase in stirring intensity. Monodisperse spherical SiO2 particles were amorphous with perfect sphere and uniform size. Hydroxyl was detected in SiO2. Kinetic parameters were calculated, and finally the reaction rate equation of dehydrated hydroxyl was obtained. | Xiaoyi Shen Yuchun Zhai Yang Sun Huimin Gu | 2010 | Journal of Materials Science & Technology2010，26，8： | 5 |
| 6 | Effect of Jianpiyiqi granule on ocular myasthenia gravis in children prepared with a formula from Traditional Chinese Medicine显示文摘OBJECTIVE: To investigate the effect of Jianpiyiqi granule, prepared according to a Traditional Chinese Medicine(TCM) formula, on the ocular type of myasthenia gravis(MG) caused by a defect in synaptic transmission at the neuromuscular junction.METHODS: A total of 155 children with ocular MG were recruited from January 2008 to January 2015.All individuals received ineffective glucocorticoid treatment prior to admission, and were given Jianpiyiqi granules(two doses per day) for 12 months.Plasma levels of acetylcholine receptor autoantibodies(ACh R-Ab), cytokines, immune parameters and clinical score were analyzed.RESULTS: After intervention with Jianpiyiqi granule for 12 months, the levels of cytokines [including interleukin(IL)-6, IL-12, IL-17], immune parameters[including immunoglobulin G(Ig G), Ig M, free triiodothyronine, free thyroxine] and ACh R-Ab were sig-nificantly decreased(P = 0.042, P = 0.049, P = 0.011,P = 0.02, P = 0.04, P = 0.03, P = 0.01; F = 21.60, P =0.000). In contrast, IL-4 levels were significantly increased(P = 0.049). The absolute clinical score after treatment declined significantly compared with before treatment(F = 33.24, P = 0.000). The effective cure rate and the total effective rate gradually increased to a maximum of 149(96.2%) and 151(97.4%) in MG patients after 12 months of treatment, respectively.CONCLUSION: The Jianpiyiqi granule treatment lowered ACh R-Ab levels and improved cytokine and other immune parameter levels, which suggests that the granule could be an ancillary treatment for ocular MG in children caused by a defect in synaptic transmission at the neuromuscular junction. | Qi Guoyan Liu Peng Gu Shanshan Yang Hongxia Dong Huimin Xue Yinping | 2017 | Journal of Traditional Chinese Medicine2017，37，4： | 4 |
| 7 | Spatial distribution of IL4 controls iNKT cell-DC crosstalk in tumors显示文摘The spatiotemporal distribution of cytokines orchestrates immune responses in vivo,yet the underlying mechanisms remain to be explored.We showed here that the spatial distribution of interleukin-4(IL4)in invariant natural killer T(iNKT)cells regulated crosstalk between iNKT cells and dendritic cells(DCs)and controlled iNKT cell-mediated T-helper type 1(Th1)responses.The persistent polarization of IL4 induced by strong lipid antigens,that is,α-galactosylceramide(αGC),caused IL4 accumulation at the immunological synapse(IS),which promoted the activation of the IL4R-STAT6(signal transducer and activator of transcription 6)pathway and production of IL12 in DCs,which enhanced interferon-γ(IFNγ)production in iNKT cells.Conversely,the nonpolarized secretion of IL4 induced by Th2 lipid antigens with a short or unsaturated chain was incapable of enhancing this iNKT cell-DC crosstalk and thus shifted the immune response to a Th2-type response.The nonpolarized secretion of IL4 in response to Th2 lipid antigens was caused by the degradation of Cdc42 in iNKT cells.Moreover,reduced Cdc42 expression was observed in tumorinfiltrating iNKT cells,which impaired IL4 polarization and disturbed iNKT cell-DC crosstalk in tumors. | Lu Wang Zhilan Liu Lili Wang Qielan Wu Xiang Li Di Xie Huimin Zhang Yongdeng Zhang Lusheng Gu Yanhong Xue Ting Yue Gang Liu Wei Ji Haiming Wei Tao Xu Li Bai | 2020 | Cellular & Molecular Immunology2020，17，5： | 4 |
| 8 | A Streptococcus suis serotype 2 caused streptococcal toxic shock syndrome(STSS) in a patient显示文摘Streptococcus suis(S.suis)is a Gram-positive,facultatively anaerobic coccus that has been implicated as the cause of a wide range of clinical disease syndromes in swine and other domestic animals.S.suis has also been implicated in disease in humans,especially among abattoir workers,swine and pork handlers.Here we report a case of streptococcal toxic shock syndrome(STSS)caused by S.suis in a 59-year-old man.Despite of intensive treatment,the patient died of shock with multiple organ failure 14 h after admission.One bacterial isolate obtained from blood culture was identified to the species level by biochemical tests and serological tests as S.suis serotype 2.Identification was confirmed by PCR amplification of genes encoding 16sRNA of S.suis and the capsule of S.suis serotype 2(cps 2J).Genes encoding virulence factors were also detected.An investigation to identify the source of S.suis revealed that several days before admission the affected man had been handling sick pigs or their meat.Transmission may occur through breaks in the skin of feet with tinea due to that no measures for personal protection was taken.This case should highten awareness of the potential for occupational exposure and human infection with S.suis. | Yefei Zhu Zhongmin Tan Lingyang Zhu Hongxing Pan Xuejun Zhang Lei Xu Huimin Qian Ling Gu Xun Ye Chen Dong Changjun Bao Rongqiang Zhu Fengcai Zhu Hua Wang | 2008 | Journal of Nanjing Medical University2008，22，5： | 3 |
| 9 | Prevalence and trends of aminoglycoside resistance in Shigella worldwide, 1999-2010显示文摘Shigellosis causes diarrheal disease in humans in both developed and developing countries, and multi-drug resistance in Shigella is an emerging problem. Understanding changing resistance patterns is important in determining appropriate antibiotic treatments. This meta-analysis systematically evaluated aminoglycoside resist-ance in Shigella. A systematic review was constructed based on MEDLINE and EMBASE databases. Randomeffect models or fixed-effect models were used based on P value considering the possibility of heterogeneity between studies for meta-analysis. Data manipulation and statistical analyses were performed using software STATA 11.0. By means of meta-analysis, we found a lower resistance to three kinds of aminoglycosides in the Europe-America areas during the 12 year study period than that of the Asia-Africa areas. Kanamycin resistance was observed to be the most common drug resistance among Shigella isolates with a prevalence of 6.88% (95%CI: 6.36%-7.43%). Comparison of data from Europe-America and Asia-Africa areas revealed that Shigella flexneri resistance was greater than the resistance calculated for Shigella sonnei. Importantly,Shigella sonnei has played a significant role in aminoglycoside-resistance in recent years. Similarly, data showed that resistance to these drugs in children was higher than the corresponding data of adults. In conclusion, aminoglycoside-resistant Shigella is not an unusual phenomenon worldwide. Distribution in Shigella resistance differs sharply based on geographic areas, periods of time and subtypes. The results from the present study highlight the need for continuous surveillance of resistance and control of antibiotic usage. | Bing Gu Xing Ke Shiyang Pan Yan Cao Ling Zhuang Rongbin Yu Huimin Qian Genyan Liu Mingqing Tong | 2013 | The Journal of Biomedical Research2013，27，2： | 3 |
| 10 | miR-20a Promotes the Axon Regeneration of DRG Neurons by Targeting Nr4a3显示文摘Dear Editor,The peripheral nervous system(PNS)regenerates more easily after injury than the central nervous system(CNS)[1].Sensory neurons in the L4–L6 dorsal root ganglia(DRGs)extend axons to form the sciatic nerve along with motor axons.The DRG neuron is one of the exceptional mature neurons whose axons can regenerate after injury. | Lili Zhao Leilei Gong Ping Li Jing Qin Lingchi Xu Qiyao Wei Huimin Xie Susu Mao Bin Yu Xiaosong Gu Songlin Zhou | 2021 | Neuroscience Bulletin2021，37，4： | 3 |
| 11 | Experimentai Research on Saturated-Gain for Soft X-Ray Laser from Neon-Like Germanium Plasma显示文摘AmpliHcation of spontaneous emissions at 19.6,23.2 and 23.6 nm have been observed by a “ultiple-Target Series Coupling”design in Ge plasma,.The combined length for four targets is up to 56mm.The gain length product(GL)of small signal is up to 18 for both lines at 23.2 and 23.6 nmt and the effective GL is 16.4 and 15.7 for these two lines respectively.This two lines are obviously tending to saturation.The divergence of x-ray laser beam is about 4 mrad. | WANG Shiji GU Yuan ZHOU GuanUn NI Yuanlong YU Songyu FU Sizu MAO Chusheng TAO Zucong CHEN Wannian LIN Zunqi FAN Dianyuan ZHANG Guoping SHENG Jiatian YANG Minglun ZHANG Tanxin SHAO Yunfeng PENG Huimin HE Xiantu YU Min | 1991 | Chinese Physics Letters1991，8，12： | 3 |
| 12 | Aptamer-functionalized PEG–PLGA nanoparticles for enhanced anti-glioma drug delivery显示文摘 | Jianwei Guo Xiaoling Gao Lina Su Huimin Xia Guangzhi Gu Zhiqing Pang Xinguo Jiang Lei Yao Jun Chen Hongzhuan Chen | 2011 | Biomaterials2011，，31： | 2 |
| 13 | Place attachment,identity and community impacts of tourism-the case of a Beijing hutong显示文摘 | Huimin Gu Chris Ryan | 2008 | Tourism management2008，29，4： | 1 |
| 14 | F3 peptide-functionalized PEG-PLA nanoparticles co-administrated with tLyp-1 peptide for anti-glioma drug delivery显示文摘 | Quanyin Hu Guangzhi Gu Zhongyang Liu Mengyin Jiang Ting Kang Deyu Miao Yifan Tu Zhiqing Pang Qingxiang Song Lei Yao Huimin Xia Hongzhan Chen Xinguo Jiang Xiaoling Gao Jun Chen | 2013 | Biomaterials2013，，4： | 1 |
| 15 | Chemical proteomic profling with photoaffinity labeling strategy identifies antimalarial targets of artemisinin显示文摘Present research on the antimalarial mechanisms of artemisinin(ART)is mainly focused on covalent drug binding targets alkylated by free radicals,while non-covalent binding targets have rarely been reported.Here,we developed a novel photoaffinity probe of ART to globally capture and identify the antimalarial target proteins of ART through chemical proteomics.The results demonstrated that ART can bind to par-asite proteins by both covalent and non-covalent modification,and these may jointly contribute to the antimalarial effects.Our work enriches the research on the antimalarial targets of ART,and provides a new perspective for further exploring the antimalarial mechanism of ART. | Peng Gao Jiayun Chen Peng Sun Jianyou Wang Huan Tang Fei Xia Liwei Gu Huimin Zhang Chen Wang Yin Kwan Wong Yinhua Zhu Chengchao Xu Jigang Wang | 2023 | Chinese Chemical Letters2023，34，12： | 1 |
| 16 | Aptamer-functionalized PEG–PLGA nanoparticles for enhanced anti-glioma drug delivery显示文摘 | Jianwei Guo Xiaoling Gao Lina Su Huimin Xia Guangzhi Gu Zhiqing Pang Xinguo Jiang Lei Yao Jun Chen Hongzhuan Chen | 2011 | Biomaterials2011，，31： | 1 |
| 17 | A critique of market analysis for suburban tourism in Beijing, China 显示文摘 | Gu Huimin Raphael R Kavanaugh | 2006 | Journal of Vacation Marketing2006，，12： | 1 |
| 18 | SeDeM Expert System: A review and new perspectives显示文摘The SeDeM Expert System was first known as a galenic pre-formulation system, which was based on the experimental research and quantitative determination of powdered substances. And the mathematical formula provided by the SeDeM Expert System has plays an important role in the study of powder properties. The system can be used not only to evaluate the powder direct compression (DC) of excipients and active pharmaceutical ingredients (API’s), but also to predict the possible formulations, so it can reduce unnecessary research and trials, and shorten the time of development. In this paper, the research development and application of SeDeM Expert System in DC was summarized, and the results showed that with a few exceptions, the system was skilled in predicting acceptable tablet formulations. Finally, the new application prospect of the system is presented, including the application of the Internet traffic and content management (iTCM) database and the new co-processed excipients. | Mingxian Gu Tianbing Guan Shulin Wan Han Zhang Xuelian Li Songtao Kong Jianbing Ren Huimin Sun Chuanyun Dai | 2019 | Journal of Pharmaceutical and Biopharmaceutical Research2019，1，1： | 1 |
| 19 | Tourism, heritage, and sacred space: Wutai Shan, China显示文摘 | Robert Shepherd Larry Yu Gu Huimin | 2012 | Journal of Heritage Tourism2012，，2： | 1 |
| 20 | Tunable and sustained-release characteristics of venlafaxine hydrochloride from chitosan–carbomer matrix tablets based on in situ formed polyelectrolyte complex film coating显示文摘The objective of this study is to design sustained-release tablets using matrix technology, which can well control the release of highly water-soluble drugs with good system robustness and simple preparation process. Taking venlafaxine hydrochloride(VH) as a drug model, the feasibility of using chitosan(CS), carbomer(CBM) combination system to achieve this goal was studied. Formulation and process variables influencing drug release from CS–CBM matrix tablets were investigated. It was found that CS–CBM combination system weakened the potential influence of CS, CBM material properties and gastric emptying time on drug release profile. Demonstrated by direct observation, differential scanning calorimetry(DSC) and Fourier transform infrared spectroscopy(FTIR), in situ self-assembled polyelectrolyte complex(PEC) film was formed on the tablet surface during gastrointestinal tract transition, which contributed to the tunable and robust control of drug release. The sustained drug release behavior was further demonstrated in vivo in Beagle dogs, with level A in vitro and in vivo correlation(IVIVC) established successfully. In conclusion, CS–CBM matrix tablets are promising system to tune and control the release of highly water-soluble drugs with good system robustness. | Xiaofei Zhang Xiangqin Gu Xiaodan Wang Huimin Wang Shirui Mao | 2018 | Asian Journal of Pharmaceutical Sciences2018，13，6： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员