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| 1 | An experimental investigation on the mechanism of fluid flow through single rough fracture of rock显示文摘The structure of fractures in nature rock appears irregular and induces complicated seepage flow behavior.The mechanism and quantitative description of fluid flow through rock fractures is a difficult subject that has been greatly concerned in the fields of geotechnical,mining,geological,and petroleum engineering.In order to probe the mechanism of fluid flow and the effects of rough structures,we conducted a few laboratory tests of fluid flow through single rough fractures,in which the Weierstrass-Mandelbrot fractal function and PMMA material were employed to produce the fracture models with various fractal roughnesses.A high-speed video camera was employed to record the fluid flow through the entire single rough fracture with a constant hydraulic pressure.The properties of fluid flow varying with the fracture roughness and the influences of the rough structure were analyzed.The components of flow resistance of a single rough fracture were discussed.A fractal model was proposed to relate the fluid resistance to the fracture roughness.A fractal equivalent permeability coefficient of a single rough fracture was formulated.This study aims to provide an experimental basis and reference for better understanding and quantitatively relating the fluid flow properties to the structures of rock fractures. | JU Yang ZHANG QinGang YANG YongMing XIE HePing GAO Feng WANG HuiJie | 2013 | Science China(Technological Sciences)2013，56，8： | 28 |
| 2 | CD147-spike protein is a novel route for SARS-CoV-2 infection to host cells显示文摘In face of the everlasting battle toward COVID-19 and the rapid evolution of SARS-CoV-2,no specific and effective drugs for treating this disease have been reported until today.Angiotensin-converting enzyme 2(ACE2),a receptor of SARS-CoV-2,mediates the virus infection by binding to spike protein.Although ACE2 is expressed in the lung,kidney,and intestine,its expressing levels are rather low,especially in the lung.Considering the great infectivity of COVID-19,we speculate that SARS-CoV-2 may depend on other routes to facilitate its infection.Here,we first discover an interaction between host cell receptor CD147 and SARS-CoV-2 spike protein.The loss of CD147 or blocking CD147 in Vero E6 and BEAS-2B cell lines by anti-CD147 antibody,Meplazumab,inhibits SARSCoV-2 amplification.Expression of human CD147 allows virus entry into non-susceptible BHK-21 cells,which can be neutralized by CD147 extracellular fragment.Viral loads are detectable in the lungs of human CD147(hCD147)mice infected with SARS-CoV-2,but not in those of virus-infected wild type mice.Interestingly,virions are observed in lymphocytes of lung tissue from a COVID-19 patient.Human T cells with a property of ACE2 natural deficiency can be infected with SARS-CoV-2 pseudovirus in a dosedependent manner,which is specifically inhibited by Meplazumab.Furthermore,CD147 mediates virus entering host cells by endocytosis.Together,our study reveals a novel virus entry route,CD147-spike protein,which provides an important target for developing specific and effective drug against COVID-19. | Ke Wang Wei Chen Zheng Zhang Yongqiang Deng Jian-Qi Lian Peng Du Ding Wei Yang Zhang Xiu-Xuan Sun Li Gong Xu Yang Lei He Lei Zhang Zhiwei Yang Jie-Jie Geng Ruo Chen Hai Zhang Bin Wang Yu-Meng Zhu Gang Nan Jian-Li Jiang Ling Li Jiao Wu Peng Lin Wan Huang Liangzhi Xie Zhao-Hui Zheng Kui Zhang Jin-Lin Miao Hong-Yong Cui Min Huang Jun Zhang Ling Fu Xiang-Min Yang Zhongpeng Zhao Shihui Sun Hongjing Gu Zhe Wang Chun-Fu Wang Yacheng Lu Ying-Ying Liu Qing-Yi Wang Huijie Bian Ping Zhu Zhi-Nan Chen | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 9 |
| 3 | Advances in research of the mid-deep South China Sea circulation显示文摘The South China Sea(SCS)is a large marginal sea connecting the Indian and Pacific oceans.Under the factors of monsoons,strait transport,and varied bathymetry,the SCS presents a three-layer structure and strong diapycnal mixing which is far greater than that in the open ocean.Theoretical analysis and observations reveal that internal tides,internal solitary waves,and strong winds are the sources of the strong mixing in the northern SCS.A major consequence of the strong mixing is an active mid-deep circulation system.This system promotes exchange of water between the SCS and adjacent oceans,and also regulates the upper layer of wind-driven circulation,making the 3 dimensional SCS circulation clearly different from that in other tropical and subtropical marginal seas.The mass transport capacity of the mid-deep circulation has a substantial impact on marine sedimentation,the biogeochemical cycle,and other processes in the SCS.This paper summarizes the recent advances in middeep sea circulation dynamics of the SCS,and discusses the opportunities and challenges in this area. | Dongxiao WANG Qiang WANG Shuqun CAI Xiaodong SHANG Shiqiu PENG Yeqiang SHU Jingen XIAO Xiaohui XIE Zhiwei ZHANG Zhiqiang LIU Jian LAN Dake CHEN Huijie XUE Guihua WANG Jianping GAN Xinong XIE Rui ZHANG Hui CHEN Qingxuan YANG | 2019 | Science China Earth Sciences2019，62，12： | 8 |
| 4 | CD147 antibody specifically and effectively inhibits infection and cytokine storm of SARS-CoV-2 and its variants delta,alpha,beta,and gamma显示文摘SARS-CoV-2 mutations contribute to increased viral transmissibility and immune escape,compromising the effectiveness of existing vaccines and neutralizing antibodies.An in-depth investigation on COVID-19 pathogenesis is urgently needed to develop a strategy against SARS-CoV-2 variants.Here,we identified CD147 as a universal receptor for SARS-CoV-2 and its variants.Meanwhile,Meplazeumab,a humanized anti-CD147 antibody,could block cellular entry of SARS-CoV-2 and its variants-alpha,beta,gamma,and delta,with inhibition rates of 68.7,75.7,52.1,52.1,and 62.3%at 60μg/ml,respectively.Furthermore,humanized CD147 transgenic mice were susceptible to SARS-CoV-2 and its two variants,alpha and beta.When infected,these mice developed exudative alveolar pneumonia,featured by immune responses involving alveoli-infiltrated macrophages,neutrophils,and lymphocytes and activation of IL-17 signaling pathway.Mechanistically,we proposed that severe COVID-19-related cytokine storm is induced by a'spike protein-CD147-CyPA signaling axis':Infection of SARS-CoV-2 through CD147 initiated the JAK-STAT pathway,which further induced expression of cyclophilin A(CyPA);CyPA reciprocally bound to CD147 and triggered MAPK pathway.Consequently,the MAPK pathway regulated the expression of cytokines and chemokines,which promoted the development of cytokine storm.Importantly,Meplazumab could effectively inhibit viral entry and inflammation caused by SARS-CoV-2 and its variants.Therefore,our findings provided a new perspective for severe COVID-19-related pathogenesis.Furthermore,the validated universal receptor for SARS-CoV-2 and its variants can be targeted for COVID-19 treatment. | Jiejie Geng Liang Chen Yufeng Yuan Ke Wang Youchun Wang Chuan Qin Guizhen Wu Ruo Chen Zheng Zhang Ding Wei Peng Du Jun Zhang Peng Lin Kui Zhang Yongqiang Deng Ke Xu Jiangning Liu Xiuxuan Sun Ting Guo Xu Yang Jiao Wu Jianli Jiang Ling Li Kun Zhang Zhe Wang Jing Zhang Qingguo Yan Hua Zhu Zhaohui Zheng Jinlin Miao Xianghui Fu Fengfan Yang Xiaochun Chen Hao Tang Yang Zhang Ying Shi Yumeng Zhu Zhuo Pei Fei Huo Xue Liang Yatao Wang Qingyi Wang Wen Xie Yirong Li Mingyan Shi Huijie Bian Ping Zhu Zhi-Nan Chen | 2021 | Signal Transduction and Targeted Therapy2021，6，10： | 6 |
| 5 | Safety and efficacy of meplazumab in healthy volunteers and COVID-19 patients: a randomized phase 1 and an exploratory phase 2 trial显示文摘Recent evidence suggests that CD147 serves as a novel receptor for severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection.Blocking CD147 via anti-CD147 antibody could suppress the in vitro SARS-CoV-2 replication.Meplazumab is a humanized anti-CD147 IgG_(2) monoclonal antibody,which may effectively prevent SARS-CoV-2 infection in coronavirus disease 2019(COVID-19)patients.Here,we conducted a randomized,double-blinded,placebo-controlled phase 1 trial to evaluate the safety,tolerability,and pharmacokinetics of meplazumab in healthy subjects,and an open-labeled,concurrent controlled add-on exploratory phase 2 study to determine the efficacy in COVID-19 patients.In phase 1 study,59 subjects were enrolled and assigned to eight cohorts,and no serious treatment-emergent adverse event(TEAE)or TEAE grade≥3 was observed.The serum and peripheral blood Cmax and area under the curve showed non-linear pharmacokinetic characteristics.No obvious relation between the incidence or titer of positive anti-drug antibody and dosage was observed in each cohort.The biodistribution study indicated that meplazumab reached lung tissue and maintained>14 days stable with the lung tissue/cardiac blood-pool ratio ranging from 0.41 to 0.32.In the exploratory phase 2 study,17 COVID-19 patients were enrolled,and 11 hospitalized patients were involved as concurrent control.The meplazumab treatment significantly improved the discharged(P=0.005)and case severity(P=0.021),and reduced the time to virus negative(P=0.045)in comparison to the control group.These results show a sound safety and tolerance of meplazumab in healthy volunteers and suggest that meplazumab could accelerate the recovery of patients from COVID-19 pneumonia with a favorable safety profile. | Huijie Bian Zhao-Hui Zheng Ding Wei Aidong Wen Zheng Zhang Jian-Qi Lian Wen-Zhen Kang Chun-Qiu Hao Jing Wang Rong-Hua Xie Ke Dong Jie-Lai Xia Jin-Lin Miao Wen Kang Guoquan Li Di Zhang Mingru Zhang Xiu-Xuan Sun Likun Ding Kui Zhang Junfeng Jia Jin Ding Zhiqin Li Yanyan Jia Lin-Na Liu Zhe Zhang Zhao-Wei Gao Hong Du Na Yao Qing Wang Ke Wang Jie-Jie Geng Bin Wang Ting Guo Ruo Chen Yu-Meng Zhu Li-Juan Wang Qian He Rui-Rui Yao Ying Shi Xiang-Min Yang Jian-Sheng Zhou Yi-Nan Ma Ya-Tao Wang Xue Liang Fei Huo Zhe Wang Yang Zhang Xu Yang Ye Zhang Lu-Hua Gao Ling Wang Xiao-Chun Chen Hao Tang Shuang-Shuang Liu Qing-Yi Wang Zhi-Nan Chen Ping Zhu | 2021 | Signal Transduction and Targeted Therapy2021，6，6： | 2 |
| 6 | 3D printed biomimetic epithelium/stroma bilayer hydrogel implant for corneal regeneration显示文摘Corneal regeneration has always been a challenge due to its sophisticated structure and undesirable keratocyte-fibroblast transformation. Herein, we propose 3D printing of a biomimetic epithelium/stroma bilayer implant for corneal regeneration. Gelatin methacrylate (GelMA) and long-chain poly(ethylene glycol) diacrylate (PEGDA) are blended to form a two-component ink, which can be printed to different mechanically robust programmed PEGDA-GelMA objects by Digital Light Processing (DLP) printing technology, due to the toughening effect of crystalline crosslinks from long-chain PEGDA on GelMA hydrogel after photo-initiated copolymerization. The printed PEGDA-GelMA hydrogels support cell adhesion, proliferation, migration, meanwhile demonstrating a high light transmittance, and an appropriate swelling degree, nutrient permeation and degradation rate. A bi-layer dome-shaped corneal scaffold consisting of rabbit corneal epithelial cells (rCECs)-laden epithelia layer and rabbit adipose-derived mesenchymal stem cells (rASCs)-laden orthogonally aligned fibrous stroma layer can be printed out with a high fidelity and robustly surgical handling ability. This bi-layer cells-laden corneal scaffold is applied in a rabbit keratoplasty model. The post-operative outcome reveals efficient sealing of corneal defects, re-epithelialization and stromal regeneration. The concerted effects of microstructure of 3D printed corneal scaffold and precisely located cells in epithelia and stroma layer provide an optimal topographical and biological microenvironment for corneal regeneration. | Binbin He Jie Wang Mengtian Xie Miaoyi Xu Yahan Zhang Huijie Hao Xiaoli Xing William Lu Quanhong Han Wenguang Liu | 2022 | Bioactive Materials2022，7，11： | 2 |
| 7 | Synthesis and hydrogen reduction of nano-sized copper tungstate powders produced by a hydrothermal method显示文摘 | Douqin Ma Jingpei Xie Jiwen Li Shu Liu Fengmei Wang Huijie Zhang Wenyan Wang Aiqin Wang Haoliang Sun | 2014 | International Journal of Refractory Metals and Hard Materials2014，，： | 1 |
| 8 | Formation of an anticyclonic eddy and the mechanism involved: a case study using cruise data from the northern South China Sea显示文摘Data from satellite altimetry and in situ observations together with the Hybrid Coordinate Ocean Model (HYCOM) reanalysis data were used to investigate the mechanism and formation of an anticyclonic eddy in the northeastern South China Sea (SCS). Analysis of water mass using cruise data indicated that the water captured in the eddy diff ers from those in the SCS, the Kuroshio intrusion, and the eddy-forming region. Data from sea surface height (SSH) and sea level anomaly (SLA) indicate that the eddy formed due both to the Kuroshio intrusion and the local circulation in the SCS. The Kuroshio intrusion is present at the start of the eddy growth (March 5-9) before Kuroshio leaps the Luzon Strait. The eddy then becomes larger and stronger in the absence of the Kuroshio intrusion. From the eddy budget of the HYCOM reanalysis data, the formation of the eddy goes in three steps. By the third step, the eddy had become aff ected by variations of local SCS circulation, which is more strongly than in the fi rst step in which it is aff ected more by the Kuroshio intrusion. The variability of the temperature and salinity inside the eddy provide a support to this conclusion. The water in the SCS intruded into the eddy from the southeast, which decrease the salinity gradually in the southern part of the eddy during the growth period. | HOU Huaqian XIE Qiang XUE Huijie SHU Yeqiang NAN Feng YIN Yuqi YU Fei | 2019 | Journal of Oceanology and Limnology2019，37，5： | 1 |
| 9 | Meplazumab in hospitalized adults with severe COVID-19(DEFLECT):a multicenter,seamless phase 2/3,randomized,third-party double-blind clinical trial显示文摘Meplazumab,a humanized CD147 antibody,has shown favourable safety and efficacy in our previous clinical studies.In DEFLECT(NCT04586153),167 patients with severe COVID-19 were enroled and randomized to receive three dosages of meplazumab and a placebo.Meplazumab at 0.12 mg/kg,compared to the placebo group,showed clinical benefits in significantly reducing mortality by 83.6%(2.4%vs.14.6%,p=0.0150),increasing the proportion of patients alive and discharged without supplemental oxygen(82.9%vs.70.7%,p=0.0337)and increasing the proportion of patients who achieved sustained clinical improvement(41.5%vs.31.7%).The response rate in the 0.2 mg/kg group was relatively increased by 16.0%compared with the placebo group(53.7%vs.46.3%).Meplazumab also reduced the viral loads and multiple cytokine levels.Compare with the placebo group,the 0.3 mg/kg significantly increased the virus negative rate by 40.6%(p=0.0363)and reduced IL-8 level(p=0.0460);the 0.2 mg/kg increased the negative conversion rate by 36.9%,and reduced IL-4(p=0.0365)and IL-8 levels(p=0.0484).In this study,the adverse events occurred at a comparable rate across the four groups,with no unexpected safety findings observed.In conclusion,meplazumab promoted COVID-19 convalescence and reduced mortality,viral load,and cytokine levels in severe COVID-19 population with good safety profile. | Huijie Bian Liang Chen Zhao-Hui Zheng Xiu-Xuan Sun Jie-Jie Geng Ruo Chen Ke Wang Xu Yang Shi-Rui Chen Si-Yu Chen Rong-Hua Xie Kui Zhang Jin-Lin Miao Jun-Feng Jia Hao Tang Shuang-Shuang Liu Hong-Wei Shi Yong Yang Xiao-Chun Chen Vinay Malhotra Nosheen Nasir Iffat Khanum Faisal Mahmood Saeed Hamid Claudio Marcel Berdun Stadnik Kengi Itinose Caroline Cândida Carvalho de Oliveira Cesar Dusilek Lucas Rivabem Adilson Joaquim Westheimer Cavalcante Suzara Souto Lopes Wladmir Faustino Saporito Fábio JoséConcilio Fucci Jesus Abraham Simon-Campos Ling Wang Lin-Na Liu Qing-Yi Wang Ding Wei Zheng Zhang Zhi-Nan Chen Ping Zhu | 2023 | Signal Transduction and Targeted Therapy2023，8，2： | 1 |
| 10 | Development of an up-converting phosphor technology-based 10-channel lateral flow assay for profiling antibodies against Yersinia pestis显示文摘 | Wenyan Hong Lihua Huang Haoran Wang Jianfeng Qu Zhaobiao Guo Chengke Xie Ziwen Zhu Youbao Zhang Zongmin Du Yanfeng Yan Yan Zheng Huijie Huang Ruifu Yang Lei Zhou | 2010 | Journal of Microbiological Methods2010，，2： | 1 |
| 11 | Dynamics of fertilizer-derived organic nitrogen fractions in an arable soil during a growing season显示文摘 | Huijie Lü Hongbo He Jinsong Zhao Wei Zhang Hongtu Xie Guoqing Hu Xiao Liu Yeye Wu Xudong Zhang | 2013 | 2013 (1-2)2013，，1： | 1 |
| 12 | Dynamics of fertilizer-derived organic nitrogen fractions in an arable soil during a growing season显示文摘 | Huijie Lü Hongbo He Jinsong Zhao Wei Zhang Hongtu Xie Guoqing Hu Xiao Liu Yeye Wu Xudong Zhang | 2013 | Plant and Soil (-)2013，，1： | 1 |
| 13 | Microtubule-bundling protein Spef1 enables mammalian ciliary central apparatus formation显示文摘Cilia are cellular protrusions containing nine microtubule(MT)doublets and function to propel cell movement or extracellular liquid flow through beating or sense environmental stimuli through signal transductions.Cilia require the central pair(CP)apparatus,consisting of two CP MTs covered with projections of CP proteins,for planar strokes.How the CP MTs of such‘9+2’cilia are constructed,however,remains unknown.Here we identify Spef1,an evolutionarily conserved microtubule-bundling protein,as a core CP MT regulator in mammalian cilia.Spef1 was selectively expressed in mammalian cells with 9+2 cilia and specifically localized along the CP.Its depletion in multiciliated mouse ependymal cells by RNAi completely abolished the CP MTs and markedly attenuated ciliary localizations of CP proteins such as Hydin and Spag6,resulting in rotational beat of the ependymal cilia.Spef1,which binds to MTs through its N-terminal calponin.homologous domain,formed homodimers through its C-terminal coiled coil region to bundle and stabilize MTs.Disruption of either the MT-binding or the dimerization activity abolished the ability of exogenous Spef1 to restore the structure and functions of the CP apparatus.We propose that Spefl bundles and stabilizes central MTs to enable the assembly and functions of the CP apparatus. | Jianqun Zheng Hao Liu Lei Zhu Yawen Chen Huijie Zhao Wei Zhang Fan Li Lele Xie Xiumin Yan Xueliang Zhu | 2019 | Journal of Molecular Cell Biology2019，11，1： | 1 |
| 14 | Direction Protection Based on Time-domain Travelling Wave for Flexible MTDC Grid显示文摘Fault direction identification is important for dc protection,which cannot only be used in directional pilot pro-tection,but also plays an important role in non-unit protection(for excluding backward faults).Direction criteria based on i and di/dt have been utilized in LCC-HVDC system extensively.However,the reliability and sensitivity of the protection are significantly influenced by fault transition resistance and line distribution capacitors.In a flexible MTDC grid,boundary char-acteristics of the DC inductor can be utilized to judge the fault direction.But when the DC inductor is only installed at converter outlet,this kind of direction protection cannot work.Therefore,this paper analyzes the frequency characteristic of the fault traveling wave(TW)first.On this basis,the direction protection based on the time-domain TW is proposed,which is immune from frequency-dependence line parameters.Compared with existing direction protection,it has higher operation reliability and sensitivity,and is independent of the line boundary.Finally,the feasibility and superiority of the designed direction protection are verified by simulation cases based on PSCAD/EMTDC.Index Terms-Frequency-dependent characteristic,operation sensitivity,operation reliability,traveling-wave based direction protection. | Ye Li Bin Li Jiawei He Huijie Lyu Wenbo Wang Zhongrun Xie | 2023 | CSEE Journal of Power and Energy Systems2023，9，4： | 0 |
| 15 | In-situ formation of nitrogen doped microporous carbon nanospheres derived from polystyrene as lubricant additives for anti-wear and friction reduction显示文摘This study presents a nitrogen-doped microporous carbon nanospheres(N@MCNs)prepared by a facile polymerization–carbonization process using low-cost styrene.The N element in situ introduces polystyrene(PS)nanospheres via emulsion polymerization of styrene with cyanuric chloride as crosslinking agent,and then carbonization obtains N@MCNs.The as-prepared carbon nanospheres possess the complete spherical structure and adjustable nitrogen amount by controlling the relative proportion of tetrachloromethane and cyanuric chloride.The friction performance of N@MCNs as lubricating oil additives was surveyed utilizing the friction experiment of ball-disc structure.The results showed that N@MCNs exhibit superb reduction performance of friction and wear.When the addition of N@MCNs was 0.06 wt%,the friction coefficient of PAO-10 decreased from 0.188 to 0.105,and the wear volume reduced by 94.4%.The width and depth of wear marks of N@MCNs decreased by 49.2% and 94.5%,respectively.The carrying capacity of load was rocketed from 100 to 400 N concurrently.Through the analysis of the lubrication mechanism,the result manifested that the prepared N@MCNs enter clearance of the friction pair,transform the sliding friction into the mixed friction of sliding and rolling,and repair the contact surface through the repair effect.Furthermore,the tribochemical reaction between nanoparticles and friction pairs forms a protective film containing nitride and metal oxides,which can avert direct contact with the matrix and improve the tribological properties.This experiment showed that nitrogen-doped polystyrene-based carbon nanospheres prepared by in-situ doping are the promising materials for wear resistance and reducing friction.This preparing method can be ulteriorly expanded to multi-element co-permeable materials.Nitrogen and boron co-doped carbon nanospheres(B,N@MCNs)were prepared by mixed carbonization of N-enriched PS and boric acid,and exhibited high load carrying capacity and good tribological properties. | Yixin WANG Qi LU Huijie XIE Shujuan LIU Qian YE Feng ZHOU Weimin LIU | 2024 | Friction2024，12，3： | 0 |
| 16 | A polynomial smooth epsilon-support vector regression based on cubic spline interpolation显示文摘Regression analysis is often formulated as an optimization problem with squared loss functions.Facing the challenge of the selection of the proper function class with polynomial smooth techniques applied to support vector regression models,this study takes cubic spline interpolation to generate a new polynomial smooth function |x|_ε~2 in ε-insensitive support vector regression.Theoretical analysis shows that S_ε~2-function is better than p_ε~2-function in properties,and the approximation accuracy of the proposed smoothing function is two order higher than that of classical p_ε~2-function.The experimental data shows the efficiency of the new approach. | 任斌 He Chunhong Liu Huijie Yang Lei Xie Guobo | 2014 | High Technology Letters2014，20，2： | 0 |
| 17 | Robust boron nanoplatform provokes potent tumoricidal activities via inhibiting heat shock protein显示文摘Near-infrared(NIR)-light-triggered photothermal therapy(PTT)is a promising treatment for breast cancer.However,its therapeutic efficiency is often compromised due to the heatinduced up-regulation of heat shock proteins,which confer photothermal resistance.To solve this urgent problem,PEGylated two-dimensional boron nanosheets(B-PEG)-which allow both multimodal imaging and photothermal conversion-were loaded with gambogic acid(GA),which can inhibit heat shock protein 90(Hsp90).Experimental findings indicated that this combination of B-PEG and GA could serve as an integrated drug delivery system for cancer diagnosis and treatment.It could be used to administer mild PTT as well as chemotherapy for breast cancer,provide improved anti-tumor effects,and reduce the toxicity of PTT,all while inhibiting breast cancer growth.This drug delivery system could offer a novel tool for administering chemotherapy combined with PTT while avoiding the adverse effects of traditional PTT. | Yuying Zhao Ning Liu Piaoxue Liu Taojian Fan Rui Ma Huijie Hong Xiaojia Chen Zhongjian Xie Han Zhang Qi Wang Tongkai Chen | 2022 | Asian Journal of Pharmaceutical Sciences2022，17，5： | 0 |
| 18 | An in situ-forming polyzwitterion hydrogel: Towards vitreous substitute application显示文摘Development of a biostable and biosafe vitreous substitute is highly desirable,but remains a grand challenge.Herein,we propose a novel strategy for constructing a readily administered vitreous substitute based on a thiol-acrylate clickable polyzwitterion macromonomer.A biocompatible multivinyl polycarboxybetaine(PCB-OAA)macromonomer is designed and synthesized,and mixed with dithiothreitol(DTT)via a Michael addition reaction to form a hydrogel in vitreous cavity.This resultant PCB-OAA hydrogel exhibits controllable gelation time,super anti-fouling ability against proteins and cells,excellent biocompatibility,and approximate key parameters to human vitreous body including equilibrium water content,density,optical properties,modulus.Remarkably,outperforming clinically used silicone oil in biocompatibility,this rapidly formed hydrogel in the vitreous cavity of rabbit eyes remains stable in vitreous cavity,showing an appealing ability to prevent significantly inflammatory response,fibrosis and complications such as raised intraocular pressure(IOP),and cataract formation.This zwitterionic polymer hydrogel holds great potential as a vitreous substitute. | Binbin He Jianhai Yang Yang Liu Xianhua Xie Huijie Hao Xiaoli Xing Wenguang Liu | 2021 | Bioactive Materials2021，6，10： | 0 |
| 19 | CD147-spike protein is a novel route for SARS-CoV-2 infection to host cells显示文摘In face of the everlasting battle toward COVID-19 and the rapid evolution of SARS-CoV-2,no specific and effective drugs for treating this disease have been reported until today.Angiotensin-converting enzyme 2(ACE2),a receptor of SARS-CoV-2,mediates the virus infection by binding to spike protein.Although ACE2 is expressed in the lung,kidney,and intestine,its expressing levels are rather low,especially in the lung.Considering the great infectivity of COVID-19,we speculate that SARS-CoV-2 may depend on other routes to facilitate its infection.Here,we first discover an interaction between host cell receptor CD147 and SARS-CoV-2 spike protein.The loss of CD147 or blocking CD147 in Vero E6 and BEAS-2B cell lines by anti-CD147 antibody,Meplazumab,inhibits SARSCoV-2 amplification.Expression of human CD147 allows virus entry into non-susceptible BHK-21 cells,which can be neutralized by CD147 extracellular fragment.Viral loads are detectable in the lungs of human CD147(hCD147)mice infected with SARS-CoV-2,but not in those of virus-infected wild type mice.Interestingly,virions are observed in lymphocytes of lung tissue from a COVID-19 patient.Human T cells with a property of ACE2 natural deficiency can be infected with SARS-CoV-2 pseudovirus in a dosedependent manner,which is specifically inhibited by Meplazumab.Furthermore,CD147 mediates virus entering host cells by endocytosis.Together,our study reveals a novel virus entry route,CD147-spike protein,which provides an important target for developing specific and effective drug against COVID-19. | Ke Wang Wei Chen Zheng Zhang Yongqiang Deng Jian-Qi Lian Peng Du Ding Wei Yang Zhang Xiu-Xuan Sun Li Gong Xu Yang Lei He Lei Zhang Zhiwei Yang Jie-Jie Geng Ruo Chen Hai Zhang Bin Wang Yu-Meng Zhu Gang Nan Jian-Li Jiang Ling Li Jiao Wu Peng Lin Wan Huang Liangzhi Xie Zhao-Hui Zheng Kui Zhang Jin-Lin Miao Hong-Yong Cui Min Huang Jun Zhang Ling Fu Xiang-Min Yang Zhongpeng Zhao Shihui Sun Hongjing Gu Zhe Wang Chun-Fu Wang Yacheng Lu Ying-Ying Liu Qing-Yi Wang Huijie Bian Ping ZhuZhi-Nan Chen | 2021 | Signal Transduction and Targeted Therapy2021，6，1： | 0 |
| 20 | The application of Melissa officinalis L.essential oil nanoemulsions protects sea bass(Lateolabrax japonicus)against myofibrillar protein and lipid oxidation during refrigeration显示文摘Objectives:The nutrient rich sea bass is prone to oxidation of lipid and protein during refrigeration.Materials and Methods:The research was to investigate the effect of different concentrations of Melissa officinalis L.essential oil(MOEO)nanoemulsions on myofibrillar protein(MP)and lipid oxidation in sea bass(Lateolabrax japonicus)during refrigeration at 4°C.Results:The results of thiobarbituric acid reactive substances and mitochondrial membrane potential showed that carboxymethyl chitosan/locust bean gum active coating solutions incorporating 2%MOEO nanoemulsions(C/L-2M)was the most effective in inhibiting lipid oxidation that occurred in sea bass under attack by reactive oxygen species.Low-field nuclear magnetic resonance results showed that C/L-2M maximally slowed the conversion of bound water to free water during storage.The oxidation of lipids and MP disrupted the secondary and tertiary conformations of MP and accelerated protein aggregation and degradation.Conclusions:C/L-2M slowed the oxidation of lipids and proteins by inhibiting the oxidation of reactive oxygen species.C/L-2M is a very promising preservative emulsion for the preservation of sea bass. | Yao Xie Huijie Yu Xin Zhao Chuhan Bian Hao Cheng Jun Mei Jing Xie | 2023 | Food Quality and Safety2023，7，3： | 0 |
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