|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Clinical and biochemical indexes from 2019-nCoV infected patients linked to viral loads and lung injury显示文摘The outbreak of the 2019-nCoV infection began in December 2019 in Wuhan,Hubei province,and rapidly spread to many provinces in China as well as other countries.Here we report the epidemiological,clinical,laboratory,and radiological characteristics,as well as potential biomarkers for predicting disease severity in 2019-nCoV-infected patients in Shenzhen,China.All 12 cases of the 2019-nCoV-infected patients developed pneumonia and half of them developed acute respiratory distress syndrome (ARDS).The most common laboratory abnormalities were hypoalbuminemia,lymphopenia,decreased percentage of lymphocytes (LYM) and neutrophils (NEU),elevated C-reactive protein (CRP) and lactate dehydrogenase (LDH),and decreased CD8 count.The viral load of 2019-nCoV detected from patient respiratory tracts was positively linked to lung disease severity.ALB,LYM,LYM (%),LDH,NEU (%),and CRP were highly correlated to the acute lung injury.Age,viral load,lung injury score,and blood biochemistry indexes,albumin (ALB),CRP,LDH,LYM (%),LYM,and NEU (%),may be predictors of disease severity.Moreover,the Angiotensin Ⅱlevel in the plasma sample from 2019-nCoV infected patients was markedly elevated and linearly associated to viral load and lung injury.Our results suggest a number of potential diagnosis biomarkers and angiotensin receptor blocker (ARB) drugs for potential repurposing treatment of 2019-nCoV infection. | Yingxia Liu Yang Yang Cong Zhang Fengming Huang Fuxiang Wang Jing Yuan Zhaoqin Wang Jinxiu Li Jianming Li Cheng Feng Zheng Zhang Lifei Wang Ling Peng Li Chen Yuhao Qin Dandan Zhao Shuguang Tan Lu Yin Jun Xu Congzhao Zhou Chengyu Jiang Lei Liu | 2020 | Science China(Life Sciences)2020，63，3： | 157 |
| 2 | The Epitope Study on the SARS-CoV Nucleocapsid Protein显示文摘The nucleocapsid protein (N protein) has been found to be an antigenic protein in a number of coronaviruses. Whether the N protein in severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is antigenic remains to be elucidated. Using Western blot and Enzyme-linked Immunosorbent Assay (ELISA),the recombinant N proteins and the synthesized peptides derived from the N protein were screened in sera from SARS patients. All patient sera in this study displayed strong positive immunoreactivities against the recombinant N proteins,whereas normal sera gave negative immunoresponses to these proteins, indicating that the N protein of SARS-CoV is an antigenic protein. Furthermore, the epitope sites in the N protein were determined by competition experiments, in which the recombinant proteins or the synthesized peptides competed against the SARS-CoV proteins to bind to the antibodies raised in SARS sera. One epitope site located at the C-terminus was confirmed as the most antigenic region in this protein. A detailed screening of peptide with ELISA demonstrated that the amino sequence from Codons 371 to 407 was the epitope site at the C-terminus of the N protein. Understanding of the epitope sites could be very significant for developing an effective diagnostic approach to SARS. | Shuting Li, Liang Lin, Hao Wang, Jianning Yin, Yan Ren, Zhe Zhao, Jie Wen, Cuiqi Zhou, Xumin Zhang, Xiaolei Li, Jingqiang Wang, Zhengfeng Zhou, Jinxiu Liu, Jianmin Shao, Tingting Lei, Jianqiu Fang, Ningzhi Xu, and Siqi LiuBeijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China & Beijing Proteomics Institute, Beijing 101300, China | 2003 | Genomics, Proteomics & Bioinformatics2003，1，3： | 9 |
| 3 | Accuracy of triage strategies for human papillomavirus DNA-positive women in low-resource settings:A cross-sectional study in China显示文摘Objective: Care HPV is a human papillomavirus(HPV) DNA test for low-resource settings(LRS).This study assesses optimum triage strategies for care HPV-positive women in LRS.Methods: A total of 2,530 Chinese women were concurrently screened for cervical cancer with visual inspection with acetic acid(VIA),liquid-based cytology and HPV testing by physician-and self-collected care HPV,and physician-collected Hybrid Capture 2(HC2).Screen-positive women were referred to colposcopy with biopsy and endocervical curettage as necessary.HPV-positivity was defined as ≥1.0 relative light units/cutoff(RLU/CO) for both care HPV and HC2.Primary physician-HC2,physician-care HPV and self-care HPV and in sequential screening with cytology,VIA,or increased HPV test-positivity performance,stratified by age,were assessed for cervical intraepithelial neoplasia(CIN) grade 2/3 or worse(CIN2/3+) detection.Results: The sensitivities and specificities of primary HPV testing for CIN2+ were: 83.8%,88.1% for physiciancare HPV; 72.1%,88.2% for self-care HPV; and 97.1%,86.0% for HC2.Physician-care HPV test-positive women with VIA triage had a sensitivity of 30.9% for CIN2+ versus 80.9% with cytology triage.Self-care HPV testpositive women with VIA triage was 26.5% versus 66.2% with cytology triage.The sensitivity of HC2 test-positive women with VIA triage was 38.2% versus 92.6% with cytology triage.The sensitivity of physician-care HPV testing for CIN2+ decreased from 83.8% at ≥1.0 RLU/CO to 72.1% at ≥10.00 RLU/CO,while the sensitivity of selfcare HPV testing decreased from 72.1% at ≥1.0 RLU/CO to 32.4% at ≥10.00 RLU/CO; similar trends were seen with age-stratification.Conclusions: VIA and cytology triage improved specificity for CIN2/3 than no triage.Sensitivity with VIA triage was unsuitable for a mass-screening program.VIA provider training might improve this strategy.Cytology triage could be feasible where a high-quality cytology program exists.Triage of HPV test-positive women by increased test positivity cutoff adds another LRS triage option. | Margaret Wang Shangying Hu Shuang Zha o Wenhua Zhang Qinjing Pan Xun Zhang FengChen Jinxiu Han Junfei Ma Jennifer S.Smith Youlin Qiao Caihong Zhou Fanghui Zhao | 2017 | Chinese Journal of Cancer Research2017，29，6： | 6 |
| 4 | Elevated plasma levels of selective cytokines in COVID-19 patients reflect viral load and lung injury显示文摘A recent outbreak of pneumonia in Wuhan,China was found to be caused by a 2019 novel coronavirus(2019-nCoV or SARS-CoV-2 or HCoV-19).We previously reported the clinical features of 12 patients with2019-n Co V infections in Shenzhen,China.To further understand the pathogenesis of COVID-19 and find better ways to monitor and treat the disease caused by 2019-n Co V,we measured the levels of 48 cytokines in the blood plasma of those 12 COVID-19 patients.Thirty-eight out of the 48 measured cytokines in the plasma of 2019-n Co V-infected patients were significantly elevated compared to healthy individuals.Seventeen cytokines were linked to 2019-nCoV loads.Fifteen cytokines,namely M-CSF,IL-10,IFN-α2,IL-17,IL-4,IP-10,IL-7,IL-1 ra,G-CSF,IL-12,IFN-γ,IL-1α,IL-2,HGF and PDGF-BB,were strongly associated with the lung-injury Murray score and could be used to predict the disease severity of2019-n Co V infections by calculating the area under the curve of the receiver-operating characteristics.Our results suggest that 2019-nCoV infections trigger extensive changes in a wide array of cytokines,some of which could be potential biomarkers of disease severity of 2019-n Co V infections.These findings will likely improve our understanding of the immunopathologic mechanisms of this emerging disease.Our results also suggest that modulators of cytokine responses may play a therapeutic role in combating the disease once the functions of these elevated cytokines have been characterized. | Yingxia Liu Cong Zhang Fengming Huang Yang Yang Fuxiang Wang Jing Yuan Zheng Zhang Yuhao Qin Xiaoyun Li Dandan Zhao Shunwang Li Shuguang Tan Zhaoqin Wang Jinxiu Li Chenguang Shen Jianming Li Ling Peng Weibo Wu Mengli Cao Li Xing Zhixiang Xu Li Chen Congzhao Zhou William J Liu Lei Liu Chengyu Jiang | 2020 | National Science Review2020，7，6： | 6 |
| 5 | D-amino acid substitution enhances the stability of antimicrobial peptide polybia-CP显示文摘 | Fengjing Jia Jiayi Wang Jinxiu Peng Ping Zhao Ziqing Kong Kairong Wang Wenjin Yan Rui Wang | 2017 | Acta Biochimica et Biophysica Sinica2017，49，10： | 5 |
| 6 | The effect of slight to minor biodegradation on C_6 to C_7 light hydrocarbons in crude oils: a case study from Dawanqi Oilfield in the Tarim Basin,NW China显示文摘Light hydrocarbons(LHs) are one of the main petroleum fractions in crude oils, and carry much information regarding the genetic origin and alteration of crude oils. But secondary alterations—especially biodegradation—have a significant effect on the composition of LHs in crude oils. Because most of the LHs affected in oils underwent only slight biodegradation(rank 1 on the biodegradation scale), the variation of LHs can be used to describe more the refined features of biodegradation. Here,23 crude oils from the Dawanqi Oilfield in the Tarim Basin, NW China, eleven of which have been biodegraded to different extents, were analyzed in order to investigate the effect of slight to minor biodegradation on C_6–C_7 LHs.The study results showed that biodegradation resulted in the prior depletion of straight-chained alkanes, followed by branched alkanes. In slight and minor biodegraded oils,such biodegradation scale could not sufficiently affect C_6–C_7cycloalkanes. For branched C_6–C_7 alkanes, generally,monomethylalkanes are biodegraded earlier than dimethylalkanes and trimethylalkanes, which indicates that branched alkanes are more resistant to biodegradation, with the increase of substituted methyl groups on parent rings.The degree of alkylation is one of the primary controlling factors on the biodegradation of C_6–C_7 LHs. There is a particular case: although 2,2,3-trimethylbutane has a relative higher alkylation degree, 2,2-dimethylpentane is more resistant to biodegradation than 2,2,3-trimethylbutane. 2,2-Dimethylpentane is the most resistant to biodegradation in branched C_6–C_7 alkanes. Furthermore, the 2-methylpentane/3-methylpentane and 2-methylhexane/3-methylhexane ratios decreased steadily with increasing biodegradation,which implies that isomers of bilateral methyl groups are more prone to bacterial attack relative to mid-chain isomers. The position of the alkyls on the carbon skeleton is also one of the critical factors controlling the rate of biodegradation. With increasing biodegradation, Mango's LH parameters K1 values decrease and K2 values increase,the values of n-heptane and isoheptane decrease, and the indices of methylcyclohexane and cyclohexane increase.LH parameters should be applied cautiously for the biodegraded oils. Because biodegraded samples belong to slight or minor biodegraded oils, the values of n-heptane and isoheptane from Dawanqi Oilfield can better reflect and determine the ‘‘Biodegraded'' zone. When the heptane value is 0–21 and the isoheptane value is 0–2.6, the crude oil in Dawanqi Oilfield is defined as the ‘‘Biodegraded'' zone. | Lu Yang Chunming Zhang Meijun Li Jing Zhao Xuening Qi Jinxiu Du | 2016 | Acta Geochimica2016，35，2： | 5 |
| 7 | FeOOH quantum dots decorated graphene sheet:An efficient electrocatalyst for ambient N2 reduction显示文摘Electrochemical N2 reduction offers a promising alternative to the Haber-Bosch process for sustainable NH3 synthesis at ambient conditions,but it needs efficient catalysts for the N2 reduction reaction(NRR).Here,we report that FeOOH quantum dots decorated graphene sheet acts as a superior catalyst toward enhanced electrocatalytic N2 reduction to NH3 under ambient conditions.In 0.1 M LiClO4,this hybrid attains a large NH3 yield rate and a high Faradaic efficiency of 27.3µg·h^−1·mg−1cat.and 14.6%at−0.4 V vs.reversible hydrogen electrode,respectively,rivalling the current efficiency of all Fe-based NRR electrocatalysts in aqueous media.It also shows strong durability during the electrolytic process. | Xiaojuan Zhu Jinxiu Zhao Lei Ji Tongwei Wu Ting Wang Shuyan Gao Abdulmohsen Ali Alshehri Khalid Ahmed Alzahrani Yonglan Luo Yimo Xiang Baozhan Zheng Xuping Sun | 2020 | Nano Research2020，13，1： | 4 |
| 8 | Development of high-resolution chloroplast markers for intraspecific phylogeographic studies of Phaeocystis globosa显示文摘Phaeocystis globosa is an important harmful algal bloom causative species distributing widely in temperate and tropical coastal waters in the world.The morphological,physiological,and biochemical characteristics are different among geographic strains,which can not be distinguished with nuclear ribosomal DNA markers at present.Therefore,the genetic distance and phylogeographic relationships of nuclear 28S rDNA D1–D2 and ITS regions,and three chloroplast intergenic spacers(petN-trnS1,trnM1-psbA,and rbcS-rpl27)were analyzed and compared among 13 strains of P.globosa isolated from the Pacific Ocean and Atlantic Ocean in this study.In addition,the nucleotide polymorphisms of 28S rDNA D1–D2,ITS,and rbcS-rpl27 regions were evaluated in two P.globosa strains.The various levels of nucleotide polymorphism were in the nuclear 28S rDNA D1–D2 region and ITS region,but no polymorphism was in the chloroplast rbcS-rpl27 intergenic spacer.A reasonable intraspecific phylogeographic relationship was presented by rbcS-rpl27 intergenic spacer,which had the strongest distinction to geographic strains compared to those of 28S rDNA D1–D2 and ITS regions.In the phylogenetic tree of rbcS-rpl27 intergenic spacer,the two strains from the North Sea of the Atlantic Ocean were divided firstly from the species of P.globosa,and then formed an independent clade,while the other Atlantic strains and all of Pacific strains joined up to build the other clade.It was implied that at least two genetically distant populations of P.globosa existed in the Atlantic coastal regions.This study provided a high-resolution chloroplast marker to analyze intraspecific phylogeographic populations of P.globosa,and preliminarily clarified the genetic relationships of the Pacific and Atlantic strains of P.globosa. | Qingchun ZHANG Zhuang NIU Jinxiu WANG Chao LIU Fanzhou KONG Xiaokun HU Jiayu ZHAO Rencheng YU | 2021 | Journal of Oceanology and Limnology2021，39，2： | 3 |
| 9 | Cu^2+ reduces hemolytic activity of the antimicrobial peptide HMPI and enhances its trypsin resistance显示文摘Nowadays,drug-resistant microbes are becoming a serious clinical problem threatening people's health and life.An timicrobial peptides(AMPs)are believed to be pote ntial alter natives of con-ventional antibiotics to combat the threat of drug-resistant microbes.However,the susceptibility of AMPs toward proteases is one of the major problems limiting their clinical use.In the present study,we reported the effect of Cu^2+on the bioactivity of AMP HMPI.We found that the addition of Cu^2+could improve the protease resistance of AMP HMPI without affecting its bioactivity.Notably,after the binding of Cu^2+with HMPI,the hemolytic activity of HMPI was greatly decreased.In additi on,our results also demonstrated that the addition of Cu^2+in creased the production of reactive oxygen species in the fun gal cells,which may be a suppleme nt for the antifungal activity of HMPI.In con elusion,the introduction of Cu^2+may provide an inorganic strategy to improve the stability and decrease the hemolytic activity of AMP HMPI,while maintaining its antifungal activity. | Jinxiu Peng Yang Yang Ping Zhao Shuai Qiu Fengjing Jia Jiayi Wang Xiaolei Liang Ahmed Shabbir Chaudhry Peihan Xu Wenjin Yan Zhaoqing Xu Kairong Wang | 2020 | Acta Biochimica et Biophysica Sinica2020，52，6： | 2 |
| 10 | Kilogram-scale synthesis of ordered mesoporous carbons and their electrochemical performance显示文摘 | Jinxiu Wang Chunfeng Xue Yingying Lv Fan Zhang Bo Tu Dongyuan Zhao | 2011 | Carbon2011，，13： | 1 |
| 11 | A Strategy for Searching Antigenic Regions in the SARS-CoV Spike Protein显示文摘In the face of the worldwide threat of severe acute respiratory syndrome (SARS)to human life, some of the most urgent challenges are to develop fast and accurate analytical methods for early diagnosis of this disease as well as to create a safe anti-viral vaccine for prevention. To these ends, we investigated the antigenicity of the spike protein (S protein), a major structural protein in the SARS-coronavirus (SARS-CoV). Based upon the theoretical analysis for hydrophobicity of the S protein, 18 peptides were synthesized. Using Enzyme-Linked Immunosorbent Assay (ELISA), these peptides were screened in the sera from SARS patients. According to these results, two fragments of the S gene were amplified by PCR and cloned into pET-32a. Both S fragments were expressed in the BL-21 strain and further purified with an affinity chromatography. These recombinant S fragments were confirmed to have positive cross-reactions with SARS sera, either by Western blot or by ELISA. Our results demonstrated that the potential epitope regions were located at Codons 469-882 in the S protein, and one epitope site was located at Codons 599-620. Identification of antigenic regions in the SARS-CoV S protein may be important for the functional studies of this virus or the development of clinical diagnosis. | Yan Ren, Zhengfeng Zhou, Jinxiu Liu, Liang Lin, Shuting Li, Hao Wang, Ji Xia, Zhe Zhao, Jie Wen, Cuiqi Zhou, Jingqiang Wang, Jianning Yin, Ningzhi Xu, and Siqi LiuBeijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China & Beijing Proteomics Institute, Beijing 101300, China. | 2003 | Genomics, Proteomics & Bioinformatics2003，1，3： | 1 |
| 12 | SimuItane ous determination of harpagoside and cinnamic acid in rat plasma by liquid chromatography electrospray ionization mass spectrometry and its application to pharmacokinetic studies 显示文摘 | Wang SuJun Ruan JinXiu Zhao YanHong | 2008 | Biomed Chromatogr2008，22，1： | 1 |
| 13 | Laboratory Diagnosis and Monitoring the Viral Shedding of SARS-CoV-2 Infection显示文摘The worldwide epidemic of coronavirus disease 2019(COVID-19)is ongoing.Rapid and accurate detection of the causative virus SARSCoV-2 is vital for the treatment and control of COVID-19.In this study,the comparative sensitivity of different respiratory specimen types were retrospectively analyzed using 3,552 clinical samples from 410 COVID-19 patients confirmed by Guangdong CDC(Center for Disease Control and Prevention).Except for bronchoalveolar lavage fluid(BALF),the sputum possessed the highest positive rate(73.4%–87.5%),followed by nasal swabs(53.1%–85.3%)for both severe and mild cases during the first 14 days after illness onset(d.a.o.).Viral RNA could be detected in all BALF samples collected from the severe group within 14 d.a.o.and lasted up to 46 d.a.o.Moreover,although viral RNA was negative in the upper respiratory samples,it was also positive in BALF samples in most cases from the severe group during treatment.Notably,no viral RNA was detected in BALF samples from the mild group.Despite typical ground-glass opacity observed via computed tomographic scans,no viral RNA was detected in the first three or all upper respiratory tract specimens from some COVID-19 patients.In conclusion,sputum is most sensitive for routine laboratory diagnosis of COVID-19,followed by nasal swabs.Detection of viral RNA in BALF improves diagnostic accuracy in severe COVID-19 patients. | Yang Yang Minghui Yang Jing Yuan Fuxiang Wang Zhaoqin Wang Jinxiu Li Mingxia Zhang Jinli Wei Ling Peng Gary Wong Haixia Zheng Weibo Wu Chenguang Shen Mingfeng Liao Kai Feng Jianming Li Qianting Yang Juanjuan Zhao Lei Liu Yingxia Liu | 2020 | The Innovation2020，1，3： | 1 |
| 14 | simple and effective aerosol pathogen disinfection test for a flowing air disinfector显示文摘Aerosol transmission is an important disease transmission route and has been especially pertinent to hospital and biosafety laboratories during the SARS-CoV-2 pandemic.The thermal resistance of airborne SARS-CoV-2 is lower than that of Bacillus subtilis spores,which are often used to test the effectiveness of SARS-CoV-2 and other pathogen disinfection methods.Herein,we propose a new method to test the disinfection ability of a flowing air disinfector(a digital electromagnetic induction air heater)using B.subtilis spores.The study provides an alternative air disinfection test method.The new test system combined an aerosol generator and a respiratory filter designed in-house and could effectively recover spores on the filter membrane at the air outlet after passing through the flowing air disinfector.The total number of bacterial spores used in the test was within the range of 5×10^(5)–5×10^(6)colony-forming units(CFUs)specified in the technical standard for disinfection.The calculation was based on the calculation method in Air Disinfection Effect Appraisal Test in Technical Standard for Disinfection(2002 Edition).At an air speed of 3.5 m/s,we used a digital electromagnetic induction air heater to disinfect flowing air containing 4.100×10^(6)CFUs of B.subtilis spores and determined that the minimum disinfection temperature was 350℃for a killing rate of 99.99%.At 400℃,additional experiments using higher spore concentrations(4.700×10^(6)±1.871×10^(5)CFU)and a higher airspeed(4 m/s)showed that the killing rate remained>99.99%.B.subtilis spores,as a biological indicator for testing the efficiency of dry-heat sterilization,were killed by the high temperatures used in this system.The proposed method used to test the flowing air disinfector is simple,stable,and effective.This study provides a reference for the development of test systems that can assess the disinfection ability of flowing air disinfectors. | Xuling Liu Zhiran Qin Linqing Wang Xiaoting Xie Yifang Fu Jianhai Yu Zuxin Liang Xiaoen He Jingshu Li Hong Dai Jinxiu Yao Qinghua Wu Weiwei Xiao Li Zhu Chengsong Wan Bao Zhang Wei Zhao | 2023 | Journal of Biosafety and Biosecurity2023，5，1： | 0 |
| 15 | 三阴性乳腺癌基因组与转录组全景图:亚型及治疗策略显示文摘文章简介三阴性乳腺癌(triple-negative breast cancer,TNBC)是雌激素受体、孕激素受体和HER-2均为阴性的一群乳腺癌,缺乏有效靶向治疗,预后差,是乳腺癌学界的世界性难题。近年的研究表明TNBC是一个异质性很强的群体,为更全面而深入地认识这类疾病,寻找精准治疗的突破口,研究团队全面分析了465例原发性三阴性乳腺癌(TNBC)队列的临床、基因组学和转录组学数据。 | Yi-Zhou Jiang Ding Ma Chen Suo Jinxiu Shi Mengzhu Xue Xin Hu Yi Xiao Ke-Da Yu Yi-Rong Liu Ying Yu Yuanting Zheng Xiangnan Li Chenhui Zhang Pengchen Hu Jing Zhang Qi Hua Jiyang Zhang Wanwan Hou Luyao Ren Ding Bao Bingying Li Jingcheng Yang Ling Yao Wen-Jia Zuo Shen Zhao Yue Gong Yi-Xing Ren Ya-Xin Zhao Yun-Song Yang Zhenmin Niu Zhi-Gang Cao Daniel G.Stover Claire Verschraegen Virginia Kaklamani Anneleen Daemen John R.Benson Kazuaki Takabe Fan Bai Da-Qiang Li 王鹏 石乐明 黄薇 邵志敏 | 2020 | 科学新闻2020，，2： | 0 |
| 16 | Mesenchymal stromal/stem cells:breaking the deadlock in the treatment of multiple organ dysfunction syndrome显示文摘Multiple organ dysfunction syndrome(MODS)is a clinical syndrome characterized by the dysfunction of two or more systems or organs.This internal environment disorder occurs simultaneously 24 h after severe trauma,shock,or infection.MODS has a high fatality rate ranging from 20%to 100%.[1]In the development of MODS following severe trauma or infection,multiple organ system fail sequentially,involving the lungs,kidneys,liver,cardiovascular,central nervous system,gastrointestinal,immune,and the blood coagulation system.MODS has complex pathogenic factors,and there are four main pathogenesis hypotheses:(1)uncontrolled inflammation hypothesis,(2)ischemia–reperfusion injury,(3)the gastrointestinal hypothesis,and(4)the biphasic preexcitation theory.Although the current clinical treatment methods of MODS,such as inflammatory factor antibodies,highly effective anti-coagulants,renal function replacement therapy,and other treatment methods,have been improved continuously,their effectiveness in MODS treatment is not ideal.Therefore,clinical MODS treatment is faced with great challenges[Figure 1]. | Jinxiu Hu Ye Li Jie He Xiaojuan Zhao Mengdie Chen Li Ye Xinghua Pan | 2022 | Chinese Medical Journal2022，135，23： | 0 |
| 17 | Image Denoising with Adaptive Weighted Graph Filtering显示文摘Graph filtering,which is founded on the theory of graph signal processing,is proved as a useful tool for image denoising.Most graph filtering methods focus on learning an ideal lowpass filter to remove noise,where clean images are restored from noisy ones by retaining the image components in low graph frequency bands.However,this lowpass filter has limited ability to separate the low-frequency noise from clean images such that it makes the denoising procedure less effective.To address this issue,we propose an adaptive weighted graph filtering(AWGF)method to replace the design of traditional ideal lowpass filter.In detail,we reassess the existing low-rank denoising method with adaptive regularizer learning(ARLLR)from the view of graph filtering.A shrinkage approach subsequently is presented on the graph frequency domain,where the components of noisy image are adaptively decreased in each band by calculating their component significances.As a result,it makes the proposed graph filtering more explainable and suitable for denoising.Meanwhile,we demonstrate a graph filter under the constraint of subspace representation is employed in the ARLLR method.Therefore,ARLLR can be treated as a special form of graph filtering.It not only enriches the theory of graph filtering,but also builds a bridge from the low-rank methods to the graph filtering methods.In the experiments,we perform the AWGF method with a graph filter generated by the classical graph Laplacian matrix.The results show our method can achieve a comparable denoising performance with several state-of-the-art denoising methods. | Ying Chen Yibin Tang Lin Zhou Yan Zhou Jinxiu Zhu Li Zhao | 2020 | Computers, Materials & Continua2020，，8： | 0 |
| 18 | Late-stage cascade of oxidation reactions during the biosynthesis of oxalicine B in Penicillium oxalicum显示文摘Oxalicine B(1)is an a-pyrone meroterpenoid with a unique bispirocyclic ring system derived from Penicillium oxalicum.The biosynthetic pathway of 15-deoxyoxalicine B(4)was preliminarily reported in Penicillium canescens,however,the genetic base and biochemical characterization of tailoring reactions for oxalicine B(1)has remained enigmatic.In this study,we characterized three oxygenases from the metabolic pathway of oxalicine B(1),including a cytochrome P450 hydroxylase OxaL,a hydroxylating Fe(II)/a-KG-dependent dioxygenase OxaK,and a multifunctional cytochrome P450 OxaB.Intriguingly,OxaK can catalyze various multicyclic intermediates or shunt products of oxalicines with impressive substrate promiscuity.OxaB was further proven via biochemical assays to have the ability to convert 15-hydroxdecaturin A(3)to 1 with a spiro-lactone core skeleton through oxidative rearrangement.We also solved the mystery of OxaL that controls C-15 hydroxylation.Chemical investigation of the wild-type strain and deletants enabled us to identify 10 metabolites including three new compounds,and the isolated compounds displayed potent anti-influenza A virus bioactivities exhibiting IC50values in the range of 4.0-19.9μmol/L.Our studies have allowed us to propose a late-stage biosynthetic pathway for oxalicine B(1)and create downstream derivatizations of oxalicines by employing enzymatic strategies. | Tao Zhang Guowei Gu Guodong Liu Jinhua Su Zhilai Zhan Jianyuan Zhao Jinxiu Qian Guowei Cai Shan Cen Dewu Zhang Liyan Yu | 2023 | Acta Pharmaceutica Sinica B2023，13，1： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员