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| 1 | Rational development of a human antibody cocktail that deploys multiple functions to confer Pan-SARS-CoVs protection显示文摘Structural principles underlying the composition and synergistic mechanisms of protective monoclonal antibody cocktails are poorly defined.Here,we exploited antibody cooperativity to develop a therapeutic antibody cocktail against SARS-CoV-2.On the basis of our previously identified humanized cross-neutralizing antibody H014,we systematically an a lyzed a fully human n aive antibody library and rationally identified a potent neutralizing antibody partner,P17,which confers effective protection in animal model.Cryo-EM studies dissected the nature of the P17 epitope,which is SARS-CoV-2 specific and distinctly different from that of HOI4.High-resolution structure of the SARS-CoV-2 spike in complex with HOM and P17,together with functional investigations revealed that in a two-antibody cocktail,synergistic neutralization was achieved by S1 shielding and conformational locking,thereby blocking receptor attachment and viral membrane fusion,conferring high potency as well as robustness against viral mutation escape.Furthermore,cluster analysis identified a hypothetical 3rd antibody partner for further reinforcing the cocktail as pan-SARS-CoVs therapeutics. | Hangping Yao Yao Sun Yong-Qiang Deng Nan Wang Yongcong Tan Na-Na Zhang Xiao-Feng Li Chao Kong Yan-Peng Xuc Qi Chen Tian-Shu Cao Hui Zhao Xintian Yan Lei Cao Zhe Lv Dandan Zhu Rui Feng Nanping Wu Wenhai Zhang Yuhao Hu Keda Chen Rong-Rong Zhang Qingyu Lv Shihui Sun Yunhua Zhou Run Yan Guan Yang Xinglu Chanjuan Liu Xiangyun Lu Linfang Cheng Hongying Qiu Xing-Yao Huang Tianhao Weng Danrong Shi Weidong Jiang Junbin Shao Lei Wang Jie Zhang Tao Jiang Guojun Lang Cheng-Feng Qinc Lanjuan Li Xiangxi Wang | 2021 | Cell Research2021，31，1： | 5 |
| 2 | Comparison of nonhuman primates identified the suitable model for COVID-19显示文摘Identification of a suitable nonhuman primate(NHP)model of COVID-19 remains challenging.Here,we characterized severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection in three NHP species:Old World monkeys Macaca mulatta(M.mulatta)and Macaca fascicularis(M.fascicularis)and New World monkey Callithrix jacchus(C.jacchus).Infected M.mulatta and M.fascicularis showed abnormal chest radiographs,an increased body temperature and a decreased body weight.Viral genomes were detected in swab and blood samples from all animals.Viral load was detected in the pulmonary tissues of M.mulatta and M.fascicularis but not C.jacchus.Furthermore,among the three animal species,M.mulatta showed the strongest response to SARS-CoV-2,including increased inflammatory cytokine expression and pathological changes in the pulmonary tissues.Collectively,these data revealed the different susceptibilities of Old World and New World monkeys to SARS-CoV-2 and identified M.mulatta as the most suitable for modeling COVID-19. | Shuaiyao Lu Yuan Zhao Wenhai Yu Yun Yang Jiahong Gao Junbin Wang Dexuan Kuang Mengli Yang Jing Yang Chunxia Ma Jingwen Xu Xingli Qian Haiyan Li Siwen Zhao Jingmei Li Haixuan Wang Haiting Long Jingxian Zhou Fangyu Luo Kaiyun Ding Daoju Wu Yong Zhang Yinliang Dong Yuqin Liu Yinqiu Zheng Xiaochen Lin Li Jiao Huanying Zheng Qing Dai Qiangming Sun Yunzhang Hu Changwen Ke Hongqi Liu Xiaozhong Peng | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 5 |
| 3 | Pull-out Behaviour of Axially Loaded Screwed-in Threaded Rods Embedded in CLT Elements: Experimental Study显示文摘Experimental investigation on the pull-out behaviour,including the withdrawal capacity,slip stiffness and inter-facial shear stress,of axially loaded screwed-in threaded rod embedded in cross laminated timber(CLT)was con-ducted.Specimens with varying embedment length and different number of threaded rods were tested in this study.To prevent premature splitting failure of timber,some specimens were reinforced in the direction perpen-dicular to the timber grain with self-tapping screws.Test results showed that the screwed-in threaded rod con-nections exhibited good pull-out behaviour with high withdrawal capacity and slip stiffness.Within a certain range,the withdrawal capacity increases considerably with the increase of embedment length,while the average interfacial shear stress shows the opposite tendency.The self-tapping screws played an important role on reducing the splitting of the timber and improving the withdrawal capacity and slip stiffness of the screwed-in threaded rod.Additionally,for the specimens with self-tapping screw reinforcements,the improvement of withdrawal capacity and stiffness when increasing the number of threaded rods are much more obvious than that of the speci-mens without the self-tapping screws,due to the change of failure modes. | Huifeng Yang Junfeng Ji Haotian Tao Benkai Shi Junbin Hu Bo Wen | 2022 | Journal of Renewable Materials2022，10，1： | 0 |
| 4 | Research Progress in Laboratory of RIB Physics in 2019显示文摘The progress of research going on in Laboratory of Rare Isotope Beam Physics(RIB),Nuclear Physics Research Center,in 2019 is in the following three aspects. | Sun Zhiyu Yang Yanyun Zhang Xueheng Yu Yuhong Wang Shitao Tang Shuwen Hu Jun Hou Suqin Xu Shiwei Yan Duo Fang Fang Zhang Yongjie Bai Zhen Ma Junbin Ma Shaobo | 2019 | IMP & HIRFL Annual Report2019，，1： | 0 |
| 5 | Pre-treatment Drug Resistance Could Impact the 96-Week Antiretroviral Efficacy in Treatment-Naive HIV-1–Infected Patients in Guangdong,China显示文摘Background:With the high prevalence of pre-treatment drug resistance(PDR)and the potential impact to the virological inhibition,the detection of PDR was particularly necessary.This study aimed to determine the prevalence of PDR in Guangdong,China,and its impact on antiretroviral therapy(ART)in treatment-naive HIV patients.Methods:A retrospective cohort study was conducted.A total of 1936 HIV-1-infected treatment-naive patients in the clinic of the infectious department,Guangzhou Eighth People’s Hospital,between August 2018 and December 2019 were assayed for PDR mutations before initiating ART.Patients with PDR mutations(PDR arm)were screened and compared with those without drug-resistant mutations(non-PDR arm).The rate of HIV-1 virologic failure(VF)and CD4^(+)T-cell counts of the 2 arms were compared at the 96th week after ART to evaluate the impact of PDR on the efficacy of ART.Results:Pretreatment drug resistance was detected in 125 cases(6.46%)from the 1936 enrolled participants,most of which were resistant to non-nucleoside reverse transcriptase inhibitors(64.00%,80/125).One hundred and eight of 125 completed the follow-up of 96 weeks(PDR arm).In this cohort,52 patients whose ART regimen containing the resistant drug were grouped as con-PDR arm,and the remaining 56 patients whose ART regimen did not contain the resistant drug were grouped as non-con-PDR arm.A total of 125 patients without PDR were randomly selected as the control group(non-PDR arm),112 of whom had completed the 96-week followup.At the 96th week after ART initiation,7 patients(6.5%,7/108)in the PDR arm and 1 patient(0.9%,1/112)in the non-PDR arm developed VF,exhibiting a significant difference(χ^(2)=4.901,P=0.029).Meanwhile,3 patients(5.8%,3/52)in the con-PDR arm developed VF;the rate was also higher than that in the non-PDR arm,but without a significant difference(χ^(2)=3.549,P=0.095).The CD4^(+)T-cell count in the non-PDR arm increased more than the PDR arm(386.6 vs.319.1 cells/μL,t=2.448,P=0.015)or the con-PDRarm(386.6 vs.325.1 cells/μL,t=1.821,P=0.070)at 12weeks afterART.However,no significant differenceswere observed in the CD4^(+)T-cell count from the 24th week after ART onward.Conclusions:Pretreatment drug resistance was moderately prevalent in Guangdong,China,and could affect the antiretroviral efficacy during a 96-week observation period,indicating the need to closely monitor PDR before ART initiation. | Pengle Guo Yun Lan Quanmin Li Xuemei Ling Junbin Li Xiaoping Tang Fengyu Hu Weiping Cai Linghua Li | 2022 | Infectious Diseases & Immunity2022，2，4： | 0 |
| 6 | Boron nitride-enabled printing of a highly sensitive and flexible iontronic pressure sensing system for spatial mapping显示文摘Recently,flexible iontronic pressure sensors(FIPSs)with higher sensitivities and wider sensing ranges than conventional capacitive sensors have been widely investigated.Due to the difficulty of fabricating the nanostructures that are commonly used on electrodes and ionic layers by screen printing techniques,strategies for fabricating such devices using these techniques to drive their mass production have rarely been reported.Herein,for the first time,we employed a 2-dimensional(2D)hexagonal boron nitride(h-BN)as both an additive and an ionic liquid reservoir in an ionic film,making the sensor printable and significantly improving its sensitivity and sensing range through screen printing.The engineered sensor exhibited high sensitivity(S_(min)>261.4 kPa^(−1))and a broad sensing range(0.05-450 kPa),and it was capable of stable operation at a high pressure(400 kPa)for more than 5000 cycles.In addition,the integrated sensor array system allowed accurate monitoring of wrist pressure and showed great potential for health care systems.We believe that using h-BN as an additive in an ionic material for screen-printed FIPS could greatly inspire research on 2D materials for similar systems and other types of sensors. | Zekun Yang Qikai Duan Junbin Zang Yunlong Zhao Weihao Zheng Ran Xiao Zhidong Zhang Liangwei Hu Guirong Wu Xueli Nan Zengxing Zhang Chenyang Xue Libo Gao | 2023 | Microsystems & Nanoengineering2023，9，3： | 0 |
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