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| 1 | A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and Dicot Plants显示文摘CRISPR/Cas9 染色体指向系统被用于许多种类。然而,为植物报导的 mostCRISPR/Cas9 系统能仅仅修改一个或一些指向地点。这里,我们报导一个柔韧的 CRISPR/Cas9 向量系统,利用植物 codon 优化了 Cas9 基因,为方便、高效率的复合染色体在 monocot 和 dicot 植物编辑。我们设计了基于 PCR 的过程很快产生多重 sgRNA 表达式盒子,它能在一个轮由金门结扎或吉布森汇编克隆被装配进二进制 CRISPR/Cas9 向量。与这个系统,我们与 85.4% 变化评估的一般水准在米饭编辑了 46 个目标地点,主要在 biallelic 和同型结合的地位。我们推想在米饭的大约 16% 同型结合的变化通过相应基于再结合的修理跟随的非相应的加入结束的机制被产生。我们也获得了一致 biallelic,在 Arabidopsis T 1 植物的异质接合、同型结合、妄想的变化。在米饭和 Arabidopsis 的指向的变化是可继承的。我们在 T 0 米饭和 T 1 由同时的指向的 Arabidopsis 植物多重(多达八) 一个基因家庭,在一条 biosynthetic 小径的多重基因,或在单个基因的多重地点的成员。这个系统为为基本研究和基因改进在植物学习多重基因和基因家庭的功能提供了一个万用的工具箱。 | Xingliang Ma Qunyu Zhang Qinlong Zhu Wei Liu Yan Chen Rong Qiu Bin Wang Zhongfang Yang Heying Li Yuru Lin Yongyao Xie Rongxin Shen Shuifu Chen Zhi Wang Yuanling Chen Jingxin Guo Letian Chen Xiucai Zhao Zhicheng Dong Yao-Guang Liu | 2015 | Molecular Plant2015，8，8： | 272 |
| 2 | Animal experiment and clinical study of effect of gamma-interferon on hepatic fibrosis显示文摘AIM To evaluate the antifibrotic effect ofdifferent doses of recombinant human Gamma-Interferon (IFN-γ) intwo rat models of hepaticfibrosis, and to observe its effect on moderatechronic hepatitis B virus fibrosis.METNODS Hepatic fibrosis was successfullyinduced in 150 and 196 rats by subcutaneousinjection of carbon tetrachloride (CCl4) andintraperitoneal injection of dimethylnitrosamine(DMN), respectively. Each of the two modeldose IFN-γ group (15 MU/kg per day, i.m. for 8group (1.67 MU/kg daily, i.m. for 8 weeks).Another group of 10 rats without any treatmentwas used as normal controls. At the end of theexperiment, semi-quantitative histopathologicalscores of inflammation and fibrosis, liver (αsmooth muscle actin (α-SMA) expression level,liver hydroxyl proline content and serumhyaluronic acid levels were compared. And 47medium chronic hepatitis B viral fibrosispatients were studied. They were given IFN-γtreatment, 100MU/day i.m. for the first threemonths and 100MU qod i.m. for the next sixmonths. Semi-quantitative pathological scoresof inflammation and fibrosis and serum hepaticfibrosis indices were compared within the 9months.RESULTS In animal experiment, thepathological fibrosis scores and liver hydroxylproline content were found to be significantlylower in rats treated with different doses of IFN-γ as compared with rats in fibrotic model groupinduced by either CCI4 or DMN, in a dose-dependent manner. For CCI4-induced model,pathological fibrosis scores in high, medium andIow doses IFN-γ groups were 5.10 ± 2.88, 7.70 ±3.53 and 8.00 ± 3.30, respectively, but the scorewas 14.60 ± 7.82 in fibrotic model group.Hydroxyl proline contents were 2.83 ± 1.18, 3.59± 1.22 and 4.80 ± 1.62, in the three IFN-γgroups, and 10.01 ± 3.23 in fibrotic model group.The difference was statistically significant(P<0.01). Similar results were found in DMN-induced model. Pathological fibrosis scoreswere 6.30±0.48, 8.10 ±2.72 and 8.30 ±2.58, inhigh, medium and Iow doses IFN-γ groups, and12.60 ± 3.57 in fibrotic model group. Hydroxylproline contents were 2.72 ± 0.58, 3.14 ± 0.71and 3.62 ± 1.02, in the three IFN-γ groups, and12.79 ± 1.54 in fibrotic model group. Thedifference was statistically significant(P<0.01). Serum hepatic fibrosis indicesdecreased significantly in the 47 patients afterIFN-γ treatment (HA: 433.38 ± 373.00 vs 281.57± 220.48; LN: 161.22± 41.02 vs 146.35 ± 44.67;PCⅢ: 192.59 ± 89.95 vs 156.98 ± 49.22; C-Ⅳ:156.30 ± 44.01 vs 139.14 ± 34.47) and thedifferences between the four indices weresignificant (P<0.05). Thirty-three patientsCONCLUSION All the three doses of IFN-γ areeffective in treating rat liver fibrosis induced byeither CCl4 or DMN, the higher the dose, thebetter the effect. And IFN-γ is effective forpatients with moderate chronic hepatitis B viralfibrosis. | Hong Lei Weng Wei Min Cai Rong Hua Liu Institute of Infectious Diseases, First Affiliated Hospital. Medical School. Zhejiang University, Hangzhou 310003, Zhejiang Province. China | 2001 | World Journal of Gastroenterology2001，7，1： | 53 |
| 3 | Enhanced secondary pollution offset reduction of primary emissions during COVID-19 lockdown in China显示文摘To control the spread of the 2019 novel coronavirus(COVID-19),China imposed nationwide restrictions on the movement of its population(lockdown)after the Chinese New Year of 2020,leading to large reductions in economic activities and associated emissions.Despite such large decreases in primary pollution,there were nonetheless several periods of heavy haze pollution in eastern China,raising questions about the well-established relationship between human activities and air quality.Here,using comprehensive measurements and modeling,we show that the haze during the COVID lockdown was driven by enhancements of secondary pollution.In particular,large decreases in NOx emissions from transportation increased ozone and nighttime NO_(3)radical formation,and these increases in atmospheric oxidizing capacity in turn facilitated the formation of secondary particulate matter.Our results,afforded by the tragic natural experiment of the COVID-19 pandemic,indicate that haze mitigation depends upon a coordinated and balanced strategy for controlling multiple pollutants. | Xin Huang Aijun Ding Jian Gao Bo Zheng Derong Zhou Ximeng Qi Rong Tang Jiaping Wang Chuanhua Ren Wei Nie Xuguang Chi Zheng Xu Liangduo Chen Yuanyuan Li Fei Che Nini Pang Haikun Wang Dan Tong Wei Qin Wei Cheng Weijing Liu Qingyan Fu Baoxian Liu Fahe Chai Steven JDavis Qiang Zhang Kebin He | 2021 | National Science Review2021，8，2： | 47 |
| 4 | Comparison of microRNA profiles of human periodontal diseased and healthy gingival tissues显示文摘MicroRNAs (miRNAs) have been demonstrated to play an important role in regulation of the immuno- inflammatory response; however, the function of miRNAs in periodontal inflammation has not been investigated. The objective of this study was to explore the properties of miRNAs in periodontal inflammation by comparing miRNA profiles of inflamed and healthy gingival tissues. Gingival tissues were obtained from 10 periodontitis patients and 10 healthy subjects. After RNA extraction, miRNA profiles were analyzed by microarray, and expression levels of selected miRNAs were confirmed by real-time quantitative reverse transcription polymerase chain reaction (RT-PCR). Analyses using two computational methods, Targetscan and MicroRNA.org, were combined to identify common targets of these miRNAs. Finally, the individual miRNA expression levels of three toll-like receptor (TLR)-related miRNAs from inflamed and healthy gingival tissues were evaluated by RT-PCR. Ninety-one miRNAs were found to be upregulated and thirty-four downregulated over two-fold in inflamed gingival tissue compared with those in healthy gingival tissue. Twelve selected inflammatory-related miRNAs, hsa-miR-126*, hsa-miR-20a, hsa-miR-142-3p, hsa-miR-19a, hsa-let-7f, hsa-miR-203, hsa-miR-17, hsa-miR-223, hsa-miR-146b, hsa-miR-146a, hsa-miR-155, and hsa-miR-205 showed comparable expression levels by microarray and real-time quantitative RT-PCR analyses. In addition, the putative inflammation targets of these miRNAs were predicted, and three that were tested (hsa-miRNA-146a, hsa-miRNA-146b, and hsa-miRNA-155), showed significant differences between inflamed and healthy gingiva. This remarkable difference in miRNA profiles between periodontal diseased and healthy gingiva implicates a probable close relationship between miRNAs and periodontal inflammation. The data also suggest that the regulation of TLRs in periodontal inflammation may involve miRNA pathways. | Yu-feng Xie Rong Shu Shao-yun Jiang Da-li Liu Xiu-li Zhang | 2011 | International Journal of Oral Science2011，3，3： | 36 |
| 5 | Tree shrew (Tupaia belangeri)as a novel laboratory disease animal model显示文摘The tree shrew(Tupaia belangeri) is a promising laboratory animal that possesses a closer genetic relationship to primates than to rodents. In addition, advantages such as small size, easy breeding, and rapid reproduction make the tree shrew an ideal subject for the study of human disease. Numerous tree shrew disease models have been generated in biological and medical studies in recent years. Here we summarize current tree shrew disease models, including models of infectious diseases, cancers, depressive disorders, drug addiction, myopia, metabolic diseases, and immune-related diseases. With the success of tree shrew transgenic technology, this species will be increasingly used in biological and medical studies in the future. | Ji Xiao Rong Liu Ce-Shi Chen | 2017 | Zoological Research2017，38，3： | 40 |
| 6 | China National Medical Products Administration approval summary:anlotinib for the treatment of advanced non-small cell lung cancer after two lines of chemotherapy显示文摘Background:On May 8,2018,the China National Medical Products Administration(NMPA)approved anlotinib,an orally administered anti-angiogenesis inhibitor,for the treatment of patients with advanced non-small cell lung can-cer(NSCLC)who have progressed after treatment with two or more lines of prior systemic chemotherapy.Main body of the abstract:China NMPA reviewed and inspected a regional double-blinded,placebo-controlled,Phase III trial comparing the overall survival(OS)of NSCLC patients between the anlotinib and placebo arms.A total of 437 patients were randomized(2:1)to receive either anlotinib(n=294)or placebo(n=143)once daily on a 2-week on and 1-week off schedule.Patients with epidermal growth factor receptor(EGFR)or activating anaplastic lymphoma kinase(ALK)genomic tumor aberrations should have disease progression on NMPA-approved therapy.Anlotinib is the first NMPA-approved drug for patients with advanced NSCLC who have progressed on at least two lines of prior systemic chemotherapies in China.The approval was based on a statistically and clinically significant improvement in median OS with anlotinib(9.46 months)compared with placebo[6.37 months;hazard ratio(HR])=0.70,95%confidence interval(CI)=0.55-0.89;two-sided log-rank P=0.002].The confirmed objective response rate(ORR)was 9.2%in the anlotinib arm and 0.7%in the placebo arm.The median duration of response(DoR)was 4.83 months,with a 95%CI of 3.31-6.97 months.The toxicity profile of anlotinib was consistent with that of known anti-angiogenesis inhibitors.Common adverse drug reactions(ADRs)in anlotinib-treated patients included hypertension(67.4%),hand-foot syndrome(43.9%),hemoptysis(14.0%),thyroid stimulating hormone(TSH)elevation(46.6%),and corrected QT interval(QTc)prolongation(26.2%).Short conclusion:Anlotinib demonstrated a clinically significant OS prolongation as a novel therapeutic option for advanced or metastatic NSCLC following at least two lines of chemotherapy. | Ming Zhou Xiaoyuan Chen Hong Zhang Lin Xia Xin Tong Limin Zou Ruimin Hao Jianhong Pan Xiao Zhao Dongmei Chen Yuanyuan Song Yueli Qi Ling Tang Zhifang Liu Rong Gao Yuankai Shi Zhimin Yang | 2019 | Cancer Communications2019，39，1： | 35 |
| 7 | Expression and significance of proapoptotic gene Bax in gastric carcinoma显示文摘INTRODUCTIONRecentinvestigationshavedemonstratedthatapoptosisplaysasignificantroleinthepathogenesisoftumors[1,2].Emphasishas... | LIU Hai Feng, LIU Wei Wen, FANG Dian Chun and MEN Rong Pu | 1999 | World Journal of Gastroenterology1999，5，1： | 32 |
| 8 | Diagenetic fluids evolution and genetic mechanism of tight sandstone gas reservoirs in Upper Triassic Xujiahe Formation in Sichuan Basin, China显示文摘The reservoirs of the Upper Triassic Xujiahe Formation in Sichuan Basin have the characteristics of low compositional maturity, low contents of cements and medium textural maturity. The general physical properties of the reservoirs are poor, with low porosity and low permeability, and there are only a few reservoirs with medium porosity and low permeability in local areas. Based on the diagenetic mineral association, a diagenetic sequence of cements is established: early calcites (or micrite siderites) →first quartz overgrowth→chlorite coatings→dissolution of feldspars and debris→chlorite linings→ second quartz overgrowth (quartz widen or filled in remain intergranular pores and solution pores)→dissolution→third quartz overgrowth (quartz filled in intergranular and intragranular solution pores)→intergrowth (ferro) calcites→dolomites→ferro (calcites) dolomites→later dissolution→veins of quartz and calcites formation. Mechanical compaction is the main factor in making the reservoirs tight in the basin, followed by the second and third quartz overgrowth. In a long-term closed system, only feld-spars and some lithic fragments are dissolved by diagenetic fluids, while intergranular cements such as quartz and calcit are not dissolved and thus have little influence on the porosity of the Xujiahe Formation. This is the third factor that may have kept the sandstones of Xujiahe Formation tight finally. The hydrocarbon was extensively generated from organic materials after the second quartz overgrowth, and selectively entered favorable reservoirs to form tight sandstone gas reservoirs. | ZHU RuKai ZOU CaiNeng ZHANG Nai WANG XueSong CHENG Rong LIU LiuHong ZHOU ChuanMin SONG LiHong | 2008 | Science China Earth Sciences2008，51，9： | 29 |
| 9 | Neuroprotection against vascular dementia after acupuncture combined with donepezil hydrochloride:P300 event related potential显示文摘Acupuncture can be used to treat various nervous system diseases.Here,168 vascular dementia patients were orally administered donepezil hydrochloride alone(5 mg/day,once a day for 56 days),or combined with acupuncture at Shenting(DU24),Tianzhu(BL10),Sishencong(Extra),Yintang(Extra),Renzhong(DU26),Neiguan(PC6),Shenmen(HT7),Fengchi(GB20),Wangu(GB12) and Baihui(DU20)(once a day for 56 days).Compared with donepezil hydrochloride alone,P300 event related potential latency was shorter with an increased amplitude in patients treated with donepezil hydrochloride and acupuncture.Mini-Mental State Examination score was also higher.Moreover,these differences in P300 latency were identified within different infarcted regions in patients treated with donepezil hydrochloride and acupuncture.These findings indicate that acupuncture combined with donepezil hydrochloride noticeably improves cognitive function in patients with vascular dementia,and exerts neuroprotective effects against vascular dementia. | Qiang Liu Xiu-juan Wang Zhe-cheng Zhang Rong Xue Ping Li Bo Li | 2016 | Neural Regeneration Research2016，11，3： | 30 |
| 10 | Extracellular heat-shock protein 70 aggravates cerulein-induced pancreatitis through toll-like receptor-4 in mice显示文摘在受不了尖锐胰腺炎的病人,的背景致病完全没被理解,并且在 vitro 的几最近的研究建议热吃惊蛋白质可能在房间发信号起一个重要作用。在胰腺炎调查细胞外的热吃惊蛋白质 70 的可能的角色( Hsp70 ),像使用费的 receptor-4 ( TLR4 )缺乏、野类型的老鼠在 导致cerulein 的胰腺炎( CIP )的功课期间与外长的 Hsp70 被管理 .Methods 尖锐胰腺炎被 cerulein 的 5 intraperitoneal 注射导致在时时间隔,然后通过尾的静脉与 recombinant 对待 Hsp70 在 cerulein 注射的开始以后的 4 个小时。随后,浆液淀粉酶和浆液 cytokines 层次被检测。胰的 Histologic 改变被评估。肿瘤坏死因素 alpha (TNF -- 一) 集中和 myeloperoxidase (军邮局) 在胰和肺的活动被分析。在胰腺的织物的原子因素 kappa B ( NF-KB )激活在 CIP 与 recombinant Hsp70 用一个敏感 RelA 连接酶的 immunosorbent assay.Results 处理被测量到野类型的老鼠在胰导致了发炎的重要恶化,浆液 cytokines 的提高的层次,肺的军邮局活动的起来规定和肺纸巾TNF-集中的增加。相反,有到TLR4缺乏的老鼠的 Hsp70 的处理在浆液 cytokines 层次上有小效果,胰腺的发炎,肺的军邮局活动和 TNF -- concentrations.Conclusions 结果建议细胞外的 HspTO 可能导致全身的煽动性的反应没有感染,在 vivo 和 TLR4 的症候群(先生) -Iike 反应可能在 CIP 的前进涉及煽动性的反应的 调停Hsp70 的激活。 | SONG Jun-min LIU Hong-xiang LI Yuan ZENG Yu-jian ZHOU Zong-guang LIU Hai-yi XU Bing WANG Ling ZHOU Bin WANG Rong | 2008 | Chinese Medical Journal2008，，15： | 27 |
| 11 | Morphological study on colonic pathology in ulcerative colitis treated by moxibustion显示文摘AIM To observe the therapeutic effect ofmoxibustion on ulcerative colitis and itsinfluence on the colonic mucosal morphology.METHODS Forty-six patients with ulcerativecolitis were randomly divided into themoxibustion with herbal medicine underneathgroup and the western medicine group.Thirtypatients were treated with the abovemoxibustion and 16 patients with Salicylayefapyridine(SASP).The colonic mucosa of 13patients in the moxibustion group was observedby colonoscopy before and after the treatment.Mucin was also analyzed by H.E and AB-PASstaining.RESULTS Seventeen patients were clinicallycured,12 were improved and 1 unchanged in themoxibustion group.In the control group,5patients were clinically cured,7 improved and 4unchanged.Thirteen patients with active UCwere taken as the subjects for histopathologicanalysis in this study.The colonic mucosallesions were remarkably improved and thecharacteristic of the mucin also changed.Inmost sections,the chronic inflammation ofmucosa was geatly ameliorated(P<0.01).Theinflammatory cell infiltratation much decreased and neutrophils,disapeared in most sections(P<0.001).The goblet cells significantlyincreased(P<0.001);crypt paracrypt abscessor mucosal ulceration was seen(P<0.001).CONCLUSION The rate of cure of ulcerative colitis by moxibustion with herbal medicine beneath is superior to that by SASP. This sort of moxibustion can effectively improve the colonic mucosal lesions and restore the proportion of mucoprotein to near normal. | Huan Gan Wu Li Bin Zhou Da Ren Shi Shi Min Liu Hui Rong Liu Bi Meng Zhang Han Ping Chen Lin Shan Zhang | 2000 | World Journal of Gastroenterology2000，6，6： | 28 |
| 12 | Sleep and Cognitive Abnormalities in Acute Minor Thalamic Infarction显示文摘In order to characterize sleep and the cognitive patterns in patients with acute minor thalamic infarction(AMTI), we enrolled 27 patients with AMTI and 12 matched healthy individuals. Questionnaires about sleep and cognition as well as polysomnography(PSG) were performed on days 14 and 90 post-stroke. Compared to healthy controls, in patients with AMTI, hyposomnia was more prevalent; sleep architecture was disrupted as indicated by decreased sleep efficiency, increased sleep latency, and decreased non-rapid eye movement sleep stages 2 and 3;more sleep-related breathing disorders occurred; and cognitive functions were worse, especially memory. While sleep apnea and long-delay memory recovered to a large extent in the patients, other sleep and cognitive function deficit often persisted. Patients with AMTI are at an increased risk for hyposomnia, sleep structure disturbance,sleep apnea, and memory deficits. Although these abnormalities improved over time, the slow and incomplete improvement suggest that early management should be considered in these patients. | Wei Wu Linyang Cui Ying Fu Qianqian Tian Lei Liu Xuan Zhang Ning Du Ying Chen Zhijun Qiu Yijun Song Fu-Dong Shi Rong Xue | 2016 | Neuroscience Bulletin2016，32，4： | 28 |
| 13 | Cluster analysis for syndromes of real-world coronary heartdisease with angina pectoris显示文摘Syndromes of coronary heart disease with angina pectoris were analyzed to provide guidance forclinical practice and to improve accuracy of traditional Chinese medicine (TCM) diagnoses and efficacy of TCMtreatment. A total of 860 cases with coronary heart disease with angina pectoris were selected from TCM ClinicalResearch Information Sharing System for TCM clinics and research. Syndromes were automatically extractedwith the cluster method and were analyzed to provide objective evidence for clinical studies. Final syndromeclassifications were recognized and confirmed by clinical experts. Popular syndromes included Qi and blooddeficiency, blood stasis and obstruction collaterals, liver depression and spleen deficiency, and Qi stagnation andblood stasis. Syndromes Qi and blood deficiency and blood stasis and obstruction collaterals accounted for 28.61%of total syndromes, whereas liver depression and spleen deficiency and Qi stagnation and blood stasis accountedfor 26.44%. The main syndrome elements comprised Qi deficiency, blood deficiency, blood stasis, and Qistagnation. | Yufeng Zhao Xueyun Yu Xinyu Cao Lin Luo Liyun He Shusong Mao Li Ma Peijing Rong Yuxue Zhao Guozheng Li Baoyan Liu | 2018 | Frontiers of Medicine2018，12，5： | 28 |
| 14 | Astragalus polysaccharide attenuates rat experimental colitis by inducing regulatory T cells in intestinal Peyer's patches显示文摘AIM: To explore probable mechanism underlying the therapeutic effect of Astragalus polysaccharide(APS) against experimental colitis.METHODS: Thirty-two Sprague-Dawley rats were randomly divided into four groups. Colitis was induced with 2, 4, 6-trinitrobenzene sulfonic acid(TNBS). The rats with colitis were treated with 400 mg/kg of APS for 7 d. The therapeutic effect was evaluated by colonic weight, weight index of the colon, colonic length, and macroscopic and histological scores. The levels of regulatory T(Treg) cells in Peyer's patches were measured by flow cytometry, and cytokines in colonic tissue homogenates were analyzed using enzyme-linked immunosorbent assay. The expression of related orphan receptor-gt(ROR-gt), IL-23 and STAT-5a was measured by Western blot.RESULTS: After 7-d treatment with APS, the weight index of the colon, colonic weight, macroscopical and histological scores were decreased, while the colonic length was increased compared with the model group. The expression of interleukin(IL)-2, IL-6, IL-17, IL-23 and ROR-gt in the colonic tissues was down-regulated, but Treg cells in Peyer's patches, TGF-β and STAT5 a in the colonic tissues were up-regulated.CONCLUSION: APS effectively ameliorates TNBSinduced experimental colitis in rats, probably through restoring the number of Treg cells, and inhibiting IL-17 levels in Peyer's patches. | Hai-mei Zhao Yan Wang Xiao-Ying Huang min-fang Huang Rong Xu Hai-Yang Yue Bu-gao Zhou Hong-Yan Huang Qi-meng Sun Duan-Yong Liu | 2016 | World Journal of Gastroenterology2016，22，11： | 28 |
| 15 | Explicit diagnosis of the local ozone production rate and the ozone-NOx-VOC sensitivities显示文摘In the troposphere, ozone is a harmful gas compound to both human health and vegetation. Ozone is produced from the reaction of NO_x(@NO + NO_2) and VOCs(volatile organic compounds) with light. Due to the highly nonlinear relationships between ozone and its precursors, proper ozone mitigation relies on the knowledge of chemical mechanisms. In this study, an observation-based method is used to simulate ozone formation and elucidate its controlling factors for a rural site on the North China Plain. The instantaneous ozone production rate is calculated utilizing a box model using the dataset obtained from the Wangdu campaign. First, the model was operated in a time-dependent mode to calculate the ozone production rate at each time stamp. The calculated ozone formation rate showed a diurnal average maximum value of 17 ppbv/h(1-h diurnal averaged). The contribution of individual peroxy radicals to ozone production was analyzed. In addition, the functional dependence of calculated P(O_3) reveals that ozone production was in a NO_x-limited regime during the campaign. Furthermore, the missing peroxy radical source will further extend NO_x-limited conditions to earlier in the day, making NO_xlimitation dominate more of a day than the current chemical model predicts. Finally, a multiple scenarios mode,also known as EKMA(empirical kinetic modeling approach), was used to simulate the response of P(O_3) to the imaginary change in precursor concentrations. We found that ozone production was in the NO_x-limited region. However, the use of NO_2 measured by the molybdenum converter and/or the absence of a peroxy radical source in the current chemical model could over-emphasize the VOC-limited effect on ozone production. | Zhaofeng Tan Keding Lu Huabin Dong Min Hu Xin Li Yuhan Liu Sihua Lu Min Shao Rong Su Haichao Wang Yusheng Wu Andreas Wahner Yuanhang Zhang | 2018 | Science Bulletin2018，63，16： | 25 |
| 16 | Single-cell RNA-seq uncovers dynamic processes and critical regulators in mouse spermatogenesis显示文摘 | Yao Chen Yuxuan Zheng Yun Gao Zhen Lin Suming Yang Tongtong Wang Qiu Wang Nannan Xie Rong Hua Mingxi Liu Jiahao Sha Michael D. Griswold Jinsong Li Fuchou Tang Ming-Han Tong | 2018 | Cell Research2018，28，9： | 26 |
| 17 | Expenditure and financial burden for the diagnosis and treatment of colorectal cancer in China:a hospital.based,multicenter,cross-sectional survey显示文摘Background: The increasing prevalence of colorectal cancer(CRC) in China and the paucity of information about relevant expenditure highlight the necessity of better understanding the financial burden and effect of CRC diagnosis and treatment. We performed a survey to quantify the direct medical and non-medical expenditure as well as the resulting financial burden of CRC patients in China.Methods: We conducted a multicenter, cross-sectional survey in 37 tertiary hospitals in 13 provinces across China between 2012 and 2014. Each enrolled patient was interviewed using a structured questionnaire. All expenditure data were inflated to the 2014 Chinese Yuan(CNY; 1 CNY = 0.163 USD). We quantified the overall expenditure and financial burden and by subgroup(hospital type, age at diagnosis, sex, education, occupation, insurance type, household income, clinical stage, pathologic type, and therapeutic regimen). We then performed generalized linear modeling to determine the factors associated with overall expenditure.Results: A total of 2356 patients with a mean age of 57.4 years were included, 57.1 % of whom were men; 13.9% of patients had stage I cancer; and the average previous-year household income was 54,525 CNY.The overall average direct expenditure per patient was estimated to be 67,408 CNY, and the expenditures for stage Ⅰ, Ⅱ, Ⅲ, and Ⅳ disease were 56,099 CNY, 59,952 CNY, 67,292 CNY, and 82,729 CNY, respectively. Non-medical expenditure accounted for 8.3%of the overall expenditure. The 1-year out-of-pocket expenditure of a newly diagnosed patient was 32,649 CNY, which accounted for 59.9% of their previous-year household income and caused 75.0% of families to suffer an unmanageable financial burden. Univariate analysis showed that financial burden and overall expenditure differed in almost all subgroups(P < 0.05), except for sex. Multivariate analysis showed that patients who were treated in specialized hospitals and those who were diagnosed with adenocarcinoma or diagnosed at a later stage were likely to spend more,whereas those with a lower household income and those who underwent surgery spent less(all P < 0.05).Conclusions: For patients in China, direct expenditure for the diagnosis and treatment of CRC seemed catastrophic,and non-medical expenditure was non-ignorable. The financial burden varied among subgroups, especially among patients with different clinical stages of disease, which suggests that, in China, CRC screening might be cost-effective. | Hui-Yao Huang Ju-Fang Shi Lan-Wei Guo Ya-Na Bai Xian-Zhen Liao Guo-iangLiu A-Yan Mao Jian-Song Ren Xiao-Jie Sun Xin-Yu Zhu Le Wang Bing-Bing Song Ling-Bin Du Lin Zhu Ji-Yong Gong Qi Zhou Yu-Qin Liu Rong Cao Ling Mai Li Lan Xiao-Hua Sun Ying Ren Jin-Yi Zhou Yuan-Zheng Wang Xiao Qi Pei-An Lou Dian Shi Ni Li Kai Zhang Jie He Min Dai | 2017 | Chinese Journal of Cancer2017，36，8： | 25 |
| 18 | Effect of low-nitrogen stress on photosynthesis and chlorophyll fluorescence characteristics of maize cultivars with different low-nitrogen tolerances显示文摘Nitrogen(N)is a critical element for plant growth and productivity that influences photosynthesis and chlorophyll fluorescence.We investigated the effect of low-N stress on leaf photosynthesis and chlorophyll fluorescence characteristics of maize cultivars with difference in tolerance to low N levels.The low-N tolerant cultivar ZH311 and low-N sensitive cultivar XY508 were used as the test materials.A field experiment(with three N levels:N0,0 kg ha–1;N1,150 kg ha–1;N2,300 kg ha–1)in Jiyanyang,Sichuan Province,China,and a hydroponic experiment(with two N levels:CK,4 mmol L–1;LN,0.04 mmol L–1)in Chengdu,Sichuan Province,China were conducted.Low-N stress significantly decreased chlorophyll content and rapid light response curves of the maximum fluorescence under light(Fm′),fluorescence instable state(Fs),non-photochemical quenching(qN),the maximum efficiency of PSII photochemistry under dark-adaption(Fv/Fm),potential activity of PSII(Fv/Fo),and actual photochemical efficiency of PSII(ΦPSII)of leaves.Further,it increased the chlorophyll(Chl)a/Chl b values and so on.The light compensation point of ZH311 decreased,while that of XY508 increased.The degree of variation of these indices in low-N tolerant cultivars was lower than that in low-N sensitive cultivars,especially at the seedling stage.Maize could increase Chl a/Chl b,apparent quantum yield and light saturation point to adapt to N stress.Compared to low-N sensitive cultivars,low-N tolerant cultivars maintained a higher net photosynthetic rate and electron transport rate to maintain stronger PSII activity,which further promoted the ability to harvest and transfer light.This might be a photosynthetic mechanism by which low-N tolerant cultivar adapt to low-N stress. | WU Ya-wei LI Qiang JIN Rong CHEN Wei LIU Xiao-lin KONG Fan-lei KE Yong-pei SHI Haichun YUAN Ji-chao | 2019 | Journal of Integrative Agriculture2019，18，6： | 25 |
| 19 | Overexpression of Aurora-A kinase promotes tumor cell proliferation and inhibits apoptosis in esophageal squamous cell carcinoma cell line显示文摘曙光 A kinase, serine/threonine 蛋白质 kinase,是潜在的 oncogene。扩大并且在人的肿瘤的几种类型在曙光 A 的表示上被发现了,包括的食道的有鳞的房间癌(ESCC ) 。在表示曙光 A 上的房间对导致 cisplatin 的 apoptosis 更抵抗,这被表明了。然而,调停的分子的机制这些效果仍然保持大部分未知。在这份报告,我们证明在通过在人的 ESCC KYSE150 的 pEGFP-Aurora-A 的稳定的 transfection 的曙光 A 的表示上,房间显著地支持了房间增长并且禁止了 cisplatin- 或紫外导致照耀的 apoptosis。caspase-3 并且 poly 的劈开(自动数据处理核糖) 在在表示房间上的曙光 A 的聚合酶(PARP ) 实质地在 cisplatin 或紫外处理以后被减少。而且,我们发现有 siRNA 的内长的曙光 A kinase 的那 silencing 实质地提高了敏感到导致 cisplatin 或在人的 ESCC EC9706 房间的紫外导致 apoptosis。在平行,在曙光 A 的表示上 potently 起来调整了 Bcl-2 的表示。而且,由废除的 siRNA 的 Bcl-2 击倒 Aurora-A 禁止 apoptosis 上的效果。总起来说,这些数据提供证据在支持房间增长并且禁止 apoptosis 的表示上的那曙光 A,建议是的新奇机制仔细与恶意的显型和 ESCC 房间的反癌症药抵抗有关。 | Xiao Xia Wang Rong Liu Shun Qian Jin Fei Yue Fan Qi Min Zhan | 2006 | Cell Research2006，16，4： | 24 |
| 20 | DESIGN METHODOLOGY OF NETWORKED SOFTWARE EVOLUTION GROWTH BASED ON SOFTWARE PATTERNS显示文摘最近,建筑群的新特征联网的一些在不同领域里吸引科学家的注意,并且导致许多种新兴的研究方向。到目前为止,大多数研究工作被结构分析在大规模软件系统在复杂网络特征的发现限制了。这篇论文论述理论基础,设计方法,算法和研究的实验结果。它第一为网络拓扑学强调进化生长的设计方法的意义面向对象(OO ) 软件系统,并且主张有各种各样的拓扑学特征的网络模型的选择和调整将在设计的进程和 OO 软件系统的实现上带不可忽略的效果。然后,我们与不同拓扑学特征和软件建模方法的发展分析“在网络模型的进化之间的否定和妥协的否定”的类似的纪律。根据软件模式的生长特征的分析,我们连续建议一个面向对象的软件网络进化生长方法和它的算法。另外,我们也基于复杂网络理论为 OO 软件系统度量标准建议参数系统。基于这些参数系统,它能分析各种各样的节点的特征,连接并且本地世界,调制网络拓扑学并且指导软件度量标准。所有这些能对详细设计,实现和表演分析有用。最后,我们集中于进化算法的应用程序并且由案例研究表明它。在实验软件工程从我们的早实验把结果与方法论作比较,我们相信建议软件工程设计方法基于复杂网络理论是一条计算软件工程途径。我们主张这个方法应该是极大地为在大规模 OO 的功能,结构和性能的设计,实现,调整和度量标准有益软件建筑群系统。 | Keqing HE Rong PENG Jing LIU Fei HE Peng LIANG Bing LI | 2006 | Journal of Systems Science & Complexity2006，19，2： | 24 |
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