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| 1 | Discovery of the eclogite and its petrography in the Northern Dabie Mountain显示文摘Eclogite from the Northern Dabie Mountain is a new finding by the authors. These eclogites in foliated perdotite are enveloped by banded gneiss and occur in the mafic-ultramafic rock belt. They are mainly composed of omphacite, garnet, diopside, orthopyroxene, amphibole, plagioclase and magnetite, and a small amount of rutile, spinel, olivin and 鏾rundum. The mineral association of peak metamorphism of the eclogite is omphacite+garnet+rutile. The existence of eclogite in the Northern Dabie Mountain implies that there was an eclogitic metamorphism prior to the granulitic facies one in the mafic-ultramafic rock belt. | XU Shutong LIU Yican SU Wen WANG Rucheng JIANG Laili WU Weiping | 2000 | Chinese Science Bulletin2000，45，3： | 39 |
| 2 | 42,573 cases of hepatectomy in China: a multicenter retrospective investigation显示文摘Hepatectomy is currently routinely performed in most hospitals in China. China owns the largest population of liver diseases and the biggest number of liver resection cases. A nationwide multicenter retrospective investigation involving 112 hospitals was performed, and focused on liver resection for patients with hepatocellular carcinoma(HCC). 42,573 cases of hepatectomy were enrolled, and 18,275 valid cases of liver resection for HCC patients were selected for statistical analysis. The epidemiology of HCC, distribution of hepatectomy, postoperative complications and prognosis were finally analyzed. In the 18,275 HCC patients,81% had hepatitis B virus infection and 10% had hepatitis C virus infection. 38% of the HCC patients had normal Alphafetoprotein(AFP) level, and other 35% had an AFP level lower than 400 ng mL^(-1). In the study period, 97% of the hepatectomy for HCC were treated with open surgery, and 23.81% had vascular exclusion techniques. The operation time was(191.7±105.6) min,the blood loss was(546.0±562.8) m L, and blood transfusion was(543.0±1,035.2) m L. The median survival for HCC patients was 631 days, with 1-, 3-, and 5-year overall survival of 73.2%, 28.8% and 19.6%, respectively. Liver cirrhosis, multiple nodules,tumor thrombosis and high AFP level were risk factors that affect postoperative survival. | Binhao Zhang Bixiang Zhang Zhiwei Zhang Zhiyong Huang Yifa Chen Minshan Chen Ping Bie Baogang Peng Liqun Wu Zhiming Wang Bo Li Jia Fan Lunxiu Qin Ping Chen Jingfeng Liu Zhe Tang Jun Niu Xinmin Yin Deyu Li Songqing He Bin Jiang Yilei Mao Weiping Zhou Xiaoping Chen | 2018 | Science China(Life Sciences)2018，61，6： | 39 |
| 3 | Roles of Chemokine Receptor 4 (CXCR4) and Chemokine Ligand 12 (CXCL12) in Metastasis of Hepatocellular Carcinoma Cells显示文摘Chemokines are involved in human hepatocellular carcinoma (HCC) carcinogenesis. However, the exact mechanism of chemokines in HCC carcinogenesis remains unknown. Here we investigated the roles of chemokine receptor 4 (CXCR4) and chemokine ligand 12 (CXCL12) in the metastasis of HCC. We found that the expression levels of CXCR4 mRNA in HCC tissues, MHCC97 cells, and HUVEC cells were 2.52 ± 1.13, 2.34 ± 1.16 and 1.63 ± 1.26, respectively and that the CXCR4 protein levels were 1.38 ± 0.13, 1.96 ± 0.32 and 1.86 ± 0.21, respectively. In contrast, CXCR4 was not detected in normal hepatic tissues. In 78 HCC patients, we also found that the concentration of CXCL12 in cancerous ascitic fluid was 783-8,364 pg/ml and that CXCL12 mRNA level in HCC metastasis portal lymph nodes was 1.21 ± 0.87 but undetectable in normal hepatic tissues. Finally we discovered that recombinant human CXCL12 could induce MHCC97 cells and HUVEC cells to migrate with chemotactic indexes (CI) of 3.9 ± 1.1 and 4.1 ± 1.6, respectively. Cancerous ascitic fluid could also induce the migration of MHCC97 cells with a CI of 1.9 ± 0.8. Thus, our data suggest that CXCR4 and CXCL12 may play an important role in the metastasis of HCC by promoting the migration of tumor cells. | Hui Liu Zeya Pan Aijun Li Siyuan Fu Yin Lei Hangyong Sun Mengchao Wu Weiping Zhou | 2008 | Cellular & Molecular Immunology2008，5，5： | 30 |
| 4 | Parishin C's prevention of Aβ_(1–42)-induced inhibition of long-term potentiation is related to NMDA receptors显示文摘The rhizome of Gastrodia elata(GE), a herb medicine, has been used for treatment of neuronal disorders in Eastern Asia for hundreds of years. Parishin C is a major ingredient of GE. In this study, the i.c.v. injection of soluble Aβ1–42oligomers model of LTP injury was used. We investigated the effects of parishin C on the improvement of LTP in soluble Aβ1–42oligomer–injected rats and the underlying electrophysiological mechanisms. Parishin C(i.p. or i.c.v.) significantly ameliorated LTP impairment induced by i.c.v. injection of soluble Aβ1–42oligomers. In cultured hippocampal neurons,soluble Aβ1–42oligomers significantly inhibited NMDAR currents while not affecting AMPAR currents and voltage-dependent currents. Pretreatment with parishin C protected NMDA receptor currents from the damage induced by Aβ. In summary, parishin C improved LTP deficits induced by soluble Aβ1–42oligomers. The protection by parishin C against Aβ-induced LTP damage might be related to NMDA receptors. | Zhihui Liu Weiping Wang Nan Feng Ling Wang Jiangong Shi Xiaoliang Wang | 2016 | Acta Pharmaceutica Sinica B2016，6，3： | 25 |
| 5 | An overview of the use of plastic-film mulching in China to increase crop yield and water-use efficiency显示文摘Since 1990,crop yields have stagnated in many parts of the world,posing a significant challenge to global food security[1].As such;innovative solutions are needed to increase the resource-use efficiency of cropping systems to produce more grains per unit area. | Dongbao Sun Haigang Li Enli Wang Wenqing He Weiping Hao Changrong Yan Yuzhong Li Xurong Mei Yanqing Zhang Zhanxiang Sun Zhikuan Jia Huaiping Zhou Tinglu Fan Xucheng Zhang Qin Liu Fengju Wang Chaochun Zhang Jianbo Shen Qingsuo Wang Fusuo Zhang | 2020 | National Science Review2020，7，10： | 19 |
| 6 | Effects of urbanization on heavy metal accumulation in surface soils, Beijing显示文摘Urbanization processes affect the accumulation of heavy metals in urban soils. Effects of urbanization on heavy metal accumulation in soils were studied using Beijing as an example. It has been suggested that the ecological function of vegetation covers shifting from natural to agricultural settings and then to urban greenbelts could increase the zinc(Zn) concentrations of soils successively. The Zn concentration of urban soils was significantly correlated to the percentage of the impervious land surface at the500 m × 500 m spatial scale. For urban parks, the age or years since the development accounted for 80% of the variances of cadmium(Cd) and Zn in soils. The population density,however, did not affect the heavy metal distributions in urban soils. To conclude, the urban age turned out to be a notable factor in quantifying heavy metal accumulation in urban soils. | Meie Wang Rui Liu Weiping Chen Chi Peng Bernd Markert | 2018 | Journal of Environmental Sciences2018，30，2： | 14 |
| 7 | Application of next-generation sequencing technology to precision medicine in cancer: joint consensus of the Tumor Biomarker Committee of the Chinese Society of Clinical Oncology显示文摘Next-generation sequencing(NGS) technology is capable of sequencing millions or billions of DNA molecules simultaneously.Therefore, it represents a promising tool for the analysis of molecular targets for the initial diagnosis of disease, monitoring of disease progression, and identifying the mechanism of drug resistance. On behalf of the Tumor Biomarker Committee of the Chinese Society of Clinical Oncology(CSCO) and the China Actionable Genome Consortium(CAGC), the present expert group hereby proposes advisory guidelines on clinical applications of NGS technology for the analysis of cancer driver genes for precision cancer therapy. This group comprises an assembly of laboratory cancer geneticists, clinical oncologists, bioinformaticians,pathologists, and other professionals. After multiple rounds of discussions and revisions, the expert group has reached a preliminary consensus on the need of NGS in clinical diagnosis, its regulation, and compliance standards in clinical sample collection. Moreover, it has prepared NGS criteria, the sequencing standard operation procedure(SOP), data analysis, report, and NGS platform certification and validation. | Xuchao Zhang Zhiyong Liang Shengyue Wang Shun Lu Yong Song Ying Cheng Jianming Ying Weiping Liu Yingyong Hou Yangqiu Li Yi Liu Jun Hou Xiufeng Liu Jianyong Shao Yanhong Tai Zheng Wang Li Fu Hui Li Xiaojun Zhou Hua Bai Mengzhao Wang You Lu Jinji Yang Wenzhao Zhong Qing Zhou Xuening Yang Jie Wang Cheng Huang Xiaoqing Liu Xiaoyan Zhou Shirong Zhang Hongxia Tian Yu Chen Ruibao Ren Ning Liao Chunyan Wu Zhongzheng Zhu Hongming Pan Yanhong Gu Liwei Wang Yunpeng Liu Suzhan Zhang Tianshu Liu Gong Chen Zhimin Shao Binghe Xu Qingyuan Zhang Ruihua Xu Lin Shen Yilong Wu | 2019 | Cancer Biology & Medicine2019，16，1： | 14 |
| 8 | Microstructure and tensile behavior of hot extruded AZ91 alloys at room temperature显示文摘AZ91 alloys were prepared by hot extrusion and its microstructure and tensile behavior at room temperature were investigated. Compared to as-cast ingot, the grain size of hot-extruded material is morerefined, the intermetallic phase Mg17Al12 is broken and dispersed discontinuously. Both strength and elongation of AZ91 are improved by hotextrusion. Tensile behavior and fracture surface of the experimental material were studied. Due to the change in microstructure, the fracture mechanism of extruded material is different from that of as-cast ingot, the latter is mainly a brittle fracture. Ductile fracture plays arole in hot-extruded AZ91 failure at room temperature. | Yuanyuan Li, Datong Zhang, Ying Liu, Yan Long, and Weiping ChenCollege of Mechanical Engineering, South China University of Technology Guangzhou 510640, China | 2002 | Journal of University of Science and Technology Beijing2002，9，5： | 10 |
| 9 | Post-collisional extensional and thrust-nappe structures in northern part of Dabie Mountains显示文摘Thrust-nappe structures and extensional struc-tures simultaneously occur in the northern part of the Dabie Mountains. The systematic structural study reveals that extensional structures along the Mozitan-Xiaotian fault and thrust-nappe structures that take the Jinzhai-Shucheng fault as their frontal thrust share the same shear sense, and display a transitional relationship from the ductile exten- sional structure of deep level in the south to the ductile-brit- tle and brittle thrust-nappe structure of shallow level in the north. The extensional and thrust-nappe structures in the region are explained to result from post-collisional processes by the continuous subduction of the Yangtze continental block and the extension induced by the uplifting of the core part of the Dabie Mountains, which are components of the extensional structures produced in the exhumation process of the ultrahigh pressure metamorphic rocks in the Dabie Mountains. Because of the frontal blocking in the process of the north-westward spreading, the extension and detachment of the low-grade metamorphic rocks along the Mozi-tan-Xiaotian fault was transformed into the northwestward thrusting, resulting in the thrust-nappe structures. They de-veloped in the period of 200 170 Ma, maybe last till the late Jurassic. | JIANG Laili, WU Weiping, CHU Dongru, LIU Yican & ZHANG Yong Geological Survey of Anhui Province , Hefei 230001, China Correspondence should be addressed to Jiang Laili (e-mail: lailij@mail. hf.ah.cn) | 2003 | Chinese Science Bulletin2003，48，21： | 9 |
| 10 | Chronic intermittent hypoxia accelerates liver fibrosis in rats with combined hypoxia and nonalcoholic steatohepatitis via angiogenesis rather than endoplasmic reticulum stress显示文摘In the present study,we aimed to investigate the role of endoplasmic reticulum stress(ERS)and its related inflammation and angiogenesis in liver fibrosis in a rat model of combined hypoxia and nonalcoholic steatohepatitis(NASH)and to confirm whether the intervention of hypoxia-inducible factor 1α(HIF1α)can improve fibrosis.Liver histological changes and biochemical indices,HIF1α,inflammatory factors,ERS-related parameters(GRP78,CHOP,caspase-3,and caspase-12),and angiogenesis indices(VEGFA,VEGFR2,and CD34)were evaluated.Compared with the control rats,the liver tissue of rats with hypoxia and NASH had obvious NASH characteristics and hepatic fibrosis was significantly aggravated,including bridging fibrosis in some rats.The mRNA expression levels of HIF1α,VEGFA,and VEGFR2 and total immunohistochemical staining scores of VEGFR2 and CD34 were significantly increased.In addition,HIF1α silencing significantly decreased HIF1α,biochemical indices(ALT,AST,and TG),inflammatory factors(TNFα,IL6,and IL1β),and angiogenesis indices(CD34 and VEGFR2),consequently,improved the hepatic fibrosis score in the rat model of combined hypoxia and NASH.Taken together,chronic intermittent hypoxia accelerates liver fibrosis in rats with combined hypoxia and NASH via angiogenesis rather than ERS and HIF1α intervention can improve liver fibrosis,angiogenesis,inflammatory factors,and biochemical indices.Therefore,HIF1α is a key regulatory factor of liver fibrosis in rats with combined hypoxia and NASH. | Wei Wu Weiping Li Jiaojiao Wei Chunsheng Wang Yunliang Yao Weihua Zhu Weimei He Weimei Zhou Jiang Liu | 2019 | Acta Biochimica et Biophysica Sinica2019，51，2： | 8 |
| 11 | Using repeated precise gps campaigns for research of present-day crustal movement and strain in the Qinghai-Tibetan Plateau显示文摘The problem of crustal movement in the Qing-hai-Tibetan Plateau is a hotstopic of the present-day international geodetic world. GPS observations in 1993, 1995 and 1997 are used to survey the three-dimensional displacement of GPS monitoring station in the Qinghai-Tibetan Plateau, and to calculate and analyze the present-day crustal movement and strain in the Qinghai-Tibetan Plateau. The result of repeated campaign shows that the crustal movement in the Qinghai-Tibetan Plateau is still compressing in the north and south directions, extending in the west and east directions, and uplifting in the vertical direction. The relative convergence velocity of the Himalayan block is approximately (19.5±1.7)mm/a, the strain velocity is about (5.5±6.0) mm/a, and the uplifting velocity is some (7.6±5.2) mm/a. The compression velocity of the Tibet block relative to Golmud in the north and south directions is (9.3±4.6) mm/a, the strain velocity of the middle part in the west and east directions is (15.6±6.3) mm/a, | LIU Jingnan XU Caijun SONG Chenghua SHI Chuang JIANG Weiping DONG Lixiang | 2001 | Chinese Science Bulletin2001，46，8： | 8 |
| 12 | The IRE1α-XBP1 pathway regulates metabolic stressinduced compensatory proliferation of pancreatic β-cells显示文摘 | Tongfu Xu Liu Yang Cheng Yan Xiaoxia Wang Ping Huang Feng Zhao Liyun Zhao Mingliang Zhang Weiping Jia Xiangdong Wang Yong Liu | 2014 | Cell Research2014，24，9： | 8 |
| 13 | BRIF and CARIF progress显示文摘China Institute of Atomic Energy (CIAE) is currently constructing Beijing rare ion beam facility (BRIF) and is proposing China advanced rare ion beam facility (CARIF). This paper is aiming at introducing the progress of BRIF project and the con ceptual design CARIF. The ISOL type facility BRIF under construction is composed of a 100 MeV 300 ?A proton cyclotron, an ISOL with mass resolution of 20000, and a super-conducting LINAC of 2 MeV/q, and will be commissioned in 2013. CARIF facility proposed is planned to use both ISOL and PF techniques. It is based on a China advanced research reactor CARR that was critical, with ISOL separation of fission fragment, post acceleration to 150 MeV/u, and fragmentation of neu tron-rich fission fragment beam like 132Sn. Such unique combination will allow CARIF to deliver beam intensity better than the best world facilities by more than one order of magnitude. | LIU WeiPing, LI ZhiHong, BAI XiXiang, WANG YouBao, GUO Bing, PENG ChaoHua, YANG Yi, SU Jun, CUI BaoQun, ZHOU ShuHua, ZHU ShengYun, XIA HaiHong, GUAN XiaLing, ZENG Sheng, ZHANG HuanQiao, CHEN YongShou, TANG HongQing, HUANG Li & FENG BeiYuan China Institute of Atomic Energy, P. O. Box 275(1), Beijing 102413, China | 2011 | Science China(Physics,Mechanics & Astronomy)2011，54，S1： | 8 |
| 14 | Sensitivity-enhanced surface plasmon resonance sensor utilizing a tungsten disulfide (WS_2) nanosheets overlayer显示文摘Tungsten disulfide(WS_2), as a representative layered transition metal dichalcogenide(TMDC) material, possesses important potential for applications in highly sensitive sensors. Here, a sensitivity-enhanced surface plasmon resonance(SPR) sensor with a metal film modified by an overlayer of WS_2 nanosheets is proposed and demonstrated. The SPR sensitivity is related to the thickness of the WS_2 overlayer, which can be tailored by coating a WS_2 ethanol suspension with different concentrations or by the number of times of repeated post-coating.Benefitting from its large surface area, high refractive index, and unique optoelectronic properties, the WS_2 nanosheet overlayer coated on the gold film significantly improves the sensing sensitivity. The highest sensitivity(up to 2459.3 nm∕RIU) in the experiment is achieved by coating the WS_2 suspension once. Compared to the case without a WS_2 overlayer, this result shows a sensitivity enhancement of 26.6%. The influence of the WS_2 nanosheet overlayer on the sensing performance improvement is analyzed and discussed. Moreover, the proposed WS_2 SPR sensor has a linear correlation coefficient of 99.76% in refractive index range of 1.333 to 1.360. Besides sensitivity enhancement, the WS_2 nanosheet overlayer is able to show additional advantages, such as protection of metal film from oxidation, tunability of the resonance wavelength region, biocompatibility, capability of vapor,and gas sensing. | HAO WANG HUI ZHANG JIANGLI DONG SHIQI HU WENGUO ZHU WENTAO QIU HUIHUI LU JIANHUI YU HEYUAN GUAN SHECHENG GAO ZHAOHUI LI WEIPING LIU MIAO HE JUN ZHANG ZHE CHEN YUNHAN LUO | 2018 | Photonics Research2018，6，6： | 8 |
| 15 | Numerical investigation of dual-porosity model with transient transfer function based on discrete-fracture model显示文摘Based on the characteristics of fractures in naturally fractured reservoir and a discrete-fracture model, a fracture network numerical well test model is developed.Bottom hole pressure response curves and the pressure field are obtained by solving the model equations with the finite-element method. By analyzing bottom hole pressure curves and the fluid flow in the pressure field, seven flow stages can be recognized on the curves. An upscaling method is developed to compare with the dual-porosity model(DPM). The comparisons results show that the DPM overestimates the inter-porosity coefficient λ and the storage factor ω. The analysis results show that fracture conductivity plays a leading role in the fluid flow. Matrix permeability influences the beginning time of flow from the matrix to fractures. Fractures density is another important parameter controlling the flow. The fracture linear flow is hidden under the large fracture density.The pressure propagation is slower in the direction of larger fracture density. | Yizhao WAN Yuewu LIU Weiping OUYANG Guofeng HAN Wenchao LIU | 2016 | Applied Mathematics and Mechanics(English Edition)2016，37，5： | 7 |
| 16 | Synthesis and applications of mesoporous Cu-Zn-Al_2O_3 catalyst for dehydrogenation of 2-butanol显示文摘A series of mesoporous Cu-Zn-Al2O3 materials have been synthesized at ambient temperature and their structure was characterized by XRD, N2 physical adsorption and TPR techniques. Their catalytic applications for the dehydrogenation of 2-butanol to methyl ethyl ketone (MEK) were evaluated in a fixed-bed flow reactor at atmospheric pressure. It is demonstrated from the XRD patterns that both the as-synthesized samples and calcined samples have the typical XRD patterns of meso-structured materials and the results of N2O chemical adsorption showed that Cu was embedded in the framework of the mesoporous materials and homogeneously dispersed in the mesoporous Cu-Zn-Al2O3 materials. The catalytic activity of 2-butanol dehydrogenation was varied in the order of CZA(10) | Deren Fang Wanzhong Ren Zhongmin Liu Xiufeng Xu Lei Xu Hongying Lǔ Weiping Liao Huimin Zhang | 2009 | Journal of Natural Gas Chemistry2009，18，2： | 7 |
| 17 | Whole-exome sequencing identifies a novel INS mutation causative of maturity-onset diabetes of the young 10显示文摘 | Jing Yan Feng Jiang Rong Zhang Tongfu Xu Zhou Zhou Wei Ren Danfeng Peng Yong Liu Cheng Hu Weiping Jia | 2017 | Journal of Molecular Cell Biology2017，9，5： | 7 |
| 18 | Constructing a high-density linkage map for Gossypium hirsutum × Gossypium barbadense and identifying QTLs for lint percentage显示文摘To introgress the good fiber quality and yield from Gossypium barbadense into a commercial Upland cotton variety, a high-density simple sequence repeat(SSR) genetic linkage map was developed from a BC1F1 population of Gossypium hirsutum×Gossypium barbadense. The map comprised 2,292 loci and covered 5115.16 centi Morgan(c M) of the cotton AD genome, with an average marker interval of 2.23 c M. Of the marker order for 1,577 common loci on this new map, 90.36% agrees well with the marker order on the D genome sequence genetic map. Compared with five published high-density SSR genetic maps, 53.14% of marker loci were newly discovered in this map. Twenty-six quantitative trait loci(QTLs) for lint percentage(LP) were identified on nine chromosomes. Nine stable or common QTLs could be used for marker-assisted selection. Fifty percent of the QTLs were from G. barbadense and increased LP by 1.07%–2.41%. These results indicated that the map could be used for screening chromosome substitution segments from G. barbadense in the Upland cotton background, identifying QTLs or genes from G. barbadense, and further developing the gene pyramiding effect for improving fiber yield and quality. | Yuzhen Shi Wentan Li Aiguo Li Ruihua Ge Baocai Zhang Junzhi Li Guangping Liu Junwen Li Aiying Liu Haihong Shang Juwu Gong Wankui Gong Zemao Yang Feiyü Tang Zhi Liu Weiping Zhu Jianxiong Jiang Xiaonan Yu Tao Wang Wei Wang Tingting Chen Kunbo Wang Zhengsheng Zhang Youlu Yuan | 2015 | Journal of Integrative Plant Biology2015，57，5： | 7 |
| 19 | Complete three-dimensional coseismic displacements due to the 2021 Maduo earthquake in Qinghai Province,China from Sentinel-1 and ALOS-2 SAR images显示文摘On 22 nd May 2021(local time),an earthquake of M_(s)7.4 struck Maduo county in Qinghai Province,China.This was the largest earthquake in China since the 2008 Wenchuan earthquake.In this study,ascending/descending Sentinel-1 and advanced land observation satellite-2(ALOS-2)synthetic aperture radar(SAR)images were used to derive the three-dimensional(3-D)coseismic displacements of this earthquake.We used the differential interferometric SAR(In SAR,DIn SAR),pixel offset-tracking(POT),multiple aperture In SAR(MAI),and burst overlap interferometry(BOI)methods to derive the displacement observations along the line-of-sight(LOS)and azimuth directions.To accurately mitigate the effect of ionospheric delay on the ALOS-2 DIn SAR observations,a polynomial fitting method was proposed to optimize range-spectrum-split-derived ionospheric phases.In addition,the 3-D displacement field was obtained by a strain model and variance component estimation(SM-VCE)method based on the high-quality SAR displacement observations.Results indicated that a left-lateral fault slip with the largest horizontal displacement of up to 2.4 m dominated this earthquake,and the small-magnitude vertical displacement with an alternating uplift/subsidence pattern along the fault trace was more concentrated in the near-fault regions.Comparison with the global navigation satellite system data indicated that the SM-VCE method can significantly improve the accuracy of the displacements compared to the classical weighted least squares method,and the incorporation of the BOI displacements can substantially benefit the accuracy of north-south displacement.In addition to the displacements,three coseismic strain invariants calculated based on the strain model parameters were also investigated.It was found that the eastern and western parts of the faults suffered more significant strains compared with the epicenter region. | Jihong LIU Jun HU Zhiwei LI Zhangfeng MA Lixin WU Weiping JIANG Guangcai FENG Jianjun ZHU | 2022 | Science China Earth Sciences2022，65，4： | 6 |
| 20 | Assessment on alien herbicide-resistant gene flow among crucifers by sexual compatibility显示文摘Cross-pollination and high cross-compatibility are frequently found in many cruciferous plants, which im-plies that there might be potential risk of alien gene flow among crucifers. In this work, the alien glyphosate-resistant gene flow between GM (Genetically modified) rapeseed vari-ety Q3 (Brassica napus) and 6 varieties from 5 cruciferous species was assessed by sexual compatibility through aniline blue fluorescence observation, manual cross, mentor pollina-tion with the mixture pollen and DNA identification. The results indicated that the compatibility indices of two B. napus varieties, B. juncea var. multiceps Tsen et Lee and Chinese cabbage (B. chinensis) (as female) with Q3 (as male) were 16.15, 12.77, 2.345 and 0.85, respectively. F1 plants were examined by PCR and all of these samples were confirmed as positive. Crossing between Orychophragmus violaceus, Des-curainia sophia (as female) and Q3 (as male) expressed high cross-incompatibility. Fluorescence microscope observation indicated that growing of pollen tube terminated at the upper 1/3 part of the style of O. violaceus and on the stigma surface of D. sophia. The mentor plants were further analyzed by PCR and all were shown to be negative. Under the neighboring growth and natural pollination conditions, the rates of gene flow to two B. napus varieties were 2.3278% and 2.1487%, respectively, B. juncea var. multiceps Tsen et Lee and Chinese cabbage were 1.0157% and 0.9236%, re-spectively. No gene flow was observed in O. violaceus and D. sophia. Frequency of gene flow was highly correlated with sexual compatibility. Due to possible alien gene flow among crucifers, special consideration and care should be taken to grow GM rapeseed. | ZHAO Xiangxiang LU Weiping QI Cunkou PU Huiming XIA Qiuxia LU Dalei LIU Geshan WANG Youping | 2005 | Chinese Science Bulletin2005，50，15： | 6 |
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