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| 1 | Clinical Characteristics of Children with Coronavirus Disease 2019 in Hubei, China显示文摘Since December 2019,COVID-19 has occurred unexpectedly and emerged as a health problem worldw ide.Despite the rapidly increasing number of cases in subsequent weeks,the clinical characteristics of pediatric cases are rarely described.A cross-sectional multicenter study was carried out in 10 hospitals across Hubei province.A total of 25 confirmed pediatric cases of COVID-19 were collected.The demographic data,epidemiological history,underlying discascs,clinical mani festations,laboratory and radiological data,treatments,and outcomes were analyzcd.Of 25 hospitalized patients with COVID-19,the boy to girl ratio was 1.27:1.The median age was 3 years.COVID-19 cases in children aged<3 years,3-6 years,and≥6-years patients were 10(40%),6(24%),and 9(36%),respectively.The most common symptoms at onset of ilness were fever(13[52%]),and dry cough(11[44%]).Chest CT images showed essential normal in 8 cases(33.3%),unilateral involvement of lungs in 5 cases(20.8%),and bilateral involvement in 11 cases(45.8%).Clinical diagnoses included upper respiratory tract infection(n=8),mild pneumonia(n=15),and critical cases(n=2).Two critical cases(8%)were given invasive mechanical ventilation,corticosteroids,and immunoglobulin.The symptoms in 24(96%)of 25 patients were alleviated and one patient had been discharged.It was concluded that children were susceptible to COVID-19 like adults,while the clinical presentations and outcomes were more favorable in children.However,children less than 3 years old accounted for majority cases and critical cases lied in this age group,which demanded extra attentions during home caring and hospitalization treatment. | Fang ZHENG Chun LIAO Qi-hong FAN Hong-bo CHEN Xue-gong ZHAO Zhong-guo XIE Xi-lin LI Chun-xi CHEN Xiao-xia LU Zhi-sheng LIU Wei LU Chun-bao CHEN Rong JIAO Ai-ming ZHANG Jin-tang WANG Xi-wei DING Yao-guang ZENG Li-ping CHENG Qing-feng HUANG Jiang WU Xi-chang LUO Zhu-jun WANG Yan-yan ZHONG Yan BAI Xiao-yan WU Run-ming JIN | 2020 | Current Medical Science2020，40，2： | 10 |
| 2 | Generation of GGTA1 biallelic knockout pigs via zinc-finger nucleases and somatic cell nuclear transfer显示文摘Genetically modified pigs are valuable models of human disease and donors of xenotransplanted organs.Conventional gene targeting in pig somatic cells is extremely inefficient.Zinc-finger nuclease(ZFN)technology has been shown to be a powerful tool for efficiently inducing mutations in the genome.However,ZFN-mediated targeting in pigs has rarely been achieved.Here,we used ZFNs to knock out the porcineα-1,3-galactosyl-transferase(GGTA1)gene,which generates Gal epitopes that trigger hyperacute immune rejection in pig-to-human transplantation.Primary pig fibroblasts were transfected with ZFNs targeting the coding region of GGTA1.Eighteen mono-allelic and four biallelic knockout cell clones were obtained after drug selection with efficiencies of 23.4%and 5.2%,respectively.The biallelic cells were used to produce cloned pigs via somatic cell nuclear transfer(SCNT).Three GGTA1 null piglets were born,and one knockout primary fibroblast cell line was established from a cloned fetus.Gal epitopes on GGTA1 null pig cells were completely eliminated from the cell membrane.Functionally,GGTA1 knockout cells were protected from complement-mediated immune attacks when incubated with human serum.This study demonstrated that ZFN is an efficient tool in creating gene-modified pigs.GGTA1 null pigs and GGTA1 null fetal fibroblasts would benefit research and pig-to-human transplantation. | BAO Lei CHEN HaiDe JONG UiMyong RIM CholHo LI WenLing LIN XiJuan ZHANG Dan LUO Qiong CUI Chun HUANG HeFeng ZHANG Yan XIAO Lei FU ZhiXin | 2014 | Science China(Life Sciences)2014，57，2： | 9 |
| 3 | Malnutrition and Quality of Life in Chinese Cancer Patients: a Clinical Study of 23,994 Subjects显示文摘Background Malnutrition is common in patients with cancer,and can negatively impact their quality of life(QoL)and even survival.However,there is currently no large data available on the prevalence of malnutrition in Chinese cancer patients.This study evaluated the prevalence of malnutrition and the QoL of Chinese patients with locoregional,recurrent or metastatic cancer.Methods We conducted a nationwide observational,multi-center,hospital-based cross-sectional study within the Chinese Society of Nutritional Oncology(CSNO)Network.All of the patients were diagnosed with one of the following 18 different types of malignant tumors:lung cancer,gastric cancer,liver cancer,colorectal cancer,breast cancer,esophageal cancer,cervical cancer,endometrial cancer,nasopharyngeal carcinoma,malignant lymphoma,leukemia,pancreatic cancer,ovarian cancer,prostate cancer,bladder cancer,brain cancer,biliary tract malignant tumors or gastrointestinal stromal tumors.These patients were enrolled from 72 hospitals located in different regions of China.The patients’nutritional status was evaluated based on the body mass index(BMI),loss of bodyweight,laboratory measurements and patient generated-subjective global assessment(PG-SGA)scores.The cancer patients’physical status and QoL were assessed by the Karnofsky Performance Status(KPS)questionnaire and the European Organization for Research and Treatment of Cancer(EORTC)QLQ-C30 questionnaire,respectively.Results From December 2013 to April 2016,23,994 patients hospitalized for cancer treatment(such as surgery,chemotherapy or radiotherapy)were enrolled in the study.The patients included 12,494(52.9%)males and 11,124(47.1%)females.The mean age was 55.8±13.7 years.The proportions of patients in cancer stagesⅠ,Ⅱ,Ⅲ,Ⅳand uncertain were 11.5%,20.3%,27.5%,30.2%and 10.5%,respectively.Among the 23,994 inpatients,the proportions of patients who were underweight(BMI<18.5 kg/m2),normal(18.5 kg/m2 | Hong Xia Xu Chun Hua Song Zhen Ming Fu Chang Wang Zeng Qing Guo Yuan Lin Ying Ying Shi Wen Hu Yi Ba Su Yi Li Zeng Ning Li Kun Hua Wang Jing Wu Ying He Jia Jun Yang Cong Hua Xie Fu Xiang Zhou Xin Xia Song Gong Yan Chen Wen Jun Ma Su Xia Luo Zi Hua Chen Ming Hua Cong Hu Ma Chun Ling Zhou Wei Wang Qi Luo Yong Mei Shi Yu Mei Qi Hai Ping Jiang Wen Xian Guan Jun Qiang Chen Jia Xin Chen Yu Fang Lan Zhou Yong Dong Feng Rong Shao Tan Tao Li Jun Wen Ou Qing Chuan Zhao Jian Xiong Wu Min Weng Qing Hua Yao Jiu Wei Cui Wei Li Han Ping Shi the Investigation on Nutrition Status and Clinical Outcome of Common Cancers(INSCOC)Group | 2021 | Journal of Nutritional Oncology2021，6，1： | 8 |
| 4 | Validity of the Chinese Version of the Patient-Generated Subjective Global Assessment (PG-SGA) in Lung Cancer Patients显示文摘The prevalence of malnutrition is high among oncology patients in China. Although the Patient- Generated Subjective Global Assessment (PG-SGA) is widely recognized as an important nutritional assessment tool, it has not been validated for Chinese cancer patients. The purpose of this study was to conduct an analysis of nutrition screening and assessment data to validate the Chinese version of the PG-SGA in Chinese patients with lung cancer. This was an observational, cross-sectional study of 2,000 consecutive adult patients with lung cancer treated at several tertiary hospitals in China. Anthropometric and patient descriptive data were collected. The PG-SGA generated a score for the nutritional risk and a global rating for the nutritional status. The internal consistency, external consistency, and construct validity were evaluated, and a known-groups comparison and principal component analyses were conducted to evaluate the reliability and validity of the PG-SGA. The known-groups comparison demonstrated the ability of the PG-SGA to differentiate lung cancer patients of different nutritional and body mass index (BMI) groups. A confirmatory factor analysis supported the original four-factor structure of the PG-SGA. In terms of the internal and external consistency, the PG-SGA demonstrated intra-class correlation coefficients (ICC) up to 0.987. Alternative form validity was also demonstrated between Box 4 of the PG-SGA and the Karnofsky Performance Status score, with excellent external consistency (r = 0.972, P < 0.001). The construct validity was supported, and selected questionnaire dimensions were evident in the principal component analysis. Significant Spearman correlations (P < 0.001) were demonstrated. The Chinese version of the PG-SGA demonstrated significant reliability and sufficient exploratory properties to support its validity. It seems to be a valid tool that can be used to access the nutritional status of Chinese lung cancer patients. The validity of the PG-SGA in Chinese cancer patients warrants further investigation in other cancer types. | Zhen Ming Fu Hong Xia Xu Chun Hua Song Wei Li Zeng Qing Guo Yuan Lin Ying Ying Shi Wen Hu Yi Ba Su Yi Li Zeng Ning Li Kun Hua Wang Jing Wu Ying He Jia Jun Yang Cong Hua Xie Xin Xia Song Gong Yan Chen Wen Jun Ma Su Xia Luo Zi Hua Chen Ming Hua Cong Hu Ma Chun Ling Zhou Wei Wang Qi Luo Yong Mei Shi Yu Mei Qi Hai Ping Jiang Wen Xian Guan Jun Qiang Chen Jia Xin Chen Yu Fang Lan Zhou Yong Dong Feng Rong Shao Tan Tao Li Jun Wen Ou Qing Chuan Zhao Jian Xiong Wu Chang Wang Han Ping Shi The Investigation on Nutrition Status and Clinical Outcome of Common Cancers (INSCOC) Group | 2016 | Journal of Nutritional Oncology2016，1，1： | 7 |
| 5 | Validity of the Chinese Version of the Patient-Generated Subjective Global Assessment (PG-SGA) in Gastric Cancer Patients显示文摘Purpose Malnutrition is severe among gastric cancer patients in China. While the Patient-Generated Subjective Global Assessment (PG-SGA) is a widely used nutritional assessment tool, the validation for Chinese patients with gastric cancer has not been performed yet. The aim of this research is to validate the Chinese version of the PGSGA in Chinese gastric cancer patients by conducting analyses of nutrition screening and assessment data. Methods Two thousand consecutive adult patients with gastric cancer were included in this observational, cross-sectional study in several tertiary hospitals in China. A survey was made among those patients, which included several questionnaires and some anthropometric parameters. We calculated the parameters to validate the use of PG-SGA categorizing the nutritional status of gastric cancer patients. Then we performed statistical analysis to evaluate the internal consistency, external consistency, and construct validity. Results This study verified the ability of the PG-SGA to differentiate gastric cancer patients into different nutritional groups. The confirmatory factor analysis verified the original four-factor structure of the PG-SGA. The internal and external consistency was measured by a concordance analysis which showed an intraclass correlation coefficients (ICC) of 0.987. The correlations of the Box 4 score with the Karnofsky performance status score demonstrated alternative form validity with good external consistency (Pearson, r = 0.643, P < 0.001). All three anthropometric dimensions of the PG-SGA were supported by principal component analysis. Spearman correlations (P < 0.001) were significant in the majority of tests. Conclusion The Chinese version of the PG-SGA is a valid tool to access the patients’ nutritional status for Chinese gastric patients. | Zhen Ming Fu Hong Xia Xu Chun Hua Song Wei Li Zeng Qing Guo Yuan Lin Ying Ying Shi Wen Hu Yi Ba Su Yi Li Zeng Ning Li Kun Hua Wang Jing Wu Ying He Jia Jun Yang Cong Hua Xie Xin Xia Song Gong Yan Chen Wen Jun Ma Su Xia Luo Zi Hua Chen Ming Hua Cong Hu Ma Chun Ling Zhou Wei Wang Qi Luo Yong Mei Shi Yu Mei Qi Hai Ping Jiang Wen Xian Guan Jun Qiang Chen Jia Xin Chen Yu Fang Lan Zhou Yong Dong Feng Rong Shao Tan Tao Li Jun Wen Ou Qing Chuan Zhao Jian Xiong Wu Chang Wang Han Ping Shi The Investigation on Nutrition Status and Clinical Outcome of Common Cancers (INSCOC) Group | 2018 | Journal of Nutritional Oncology2018，3，4： | 5 |
| 6 | Searching for the Nano-Hertz Stochastic Gravitational Wave Background with the Chinese Pulsar Timing Array Data ReleaseⅠ显示文摘Observing and timing a group of millisecond pulsars with high rotational stability enables the direct detection of gravitational waves(GWs).The GW signals can be identified from the spatial correlations encoded in the times-of-arrival of widely spaced pulsar-pairs.The Chinese Pulsar Timing Array(CPTA)is a collaboration aiming at the direct GW detection with observations carried out using Chinese radio telescopes.This short article serves as a“table of contents”for a forthcoming series of papers related to the CPTA Data Release 1(CPTA DR1)which uses observations from the Five-hundred-meter Aperture Spherical radio Telescope.Here,after summarizing the time span and accuracy of CPTA DR1,we report the key results of our statistical inference finding a correlated signal with amplitude logA_(c)=-14.4_(-2.8)^(+1.0)for spectral index in the range ofα∈[-1.8,1.5]assuming a GW background(GWB)induced quadrupolar correlation.The search for the Hellings–Downs(HD)correlation curve is also presented,where some evidence for the HD correlation has been found that a 4.6σstatistical significance is achieved using the discrete frequency method around the frequency of 14 n Hz.We expect that the future International Pulsar Timing Array data analysis and the next CPTA data release will be more sensitive to the n Hz GWB,which could verify the current results. | Heng Xu Siyuan Chen Yanjun Guo Jinchen Jiang Bojun Wang Jiangwei Xu Zihan Xue RNicolas Caballero Jianping Yuan Yonghua Xu Jingbo Wang Longfei Hao Jingtao Luo Kejia Lee Jinlin Han Peng Jiang Zhiqiang Shen Min Wang Na Wang Renxin Xu Xiangping Wu Richard Manchester Lei Qian Xin Guan Menglin Huang Chun Sun Yan Zhu | 2023 | Research in Astronomy and Astrophysics2023，23，7： | 3 |
| 7 | Extension Protocol for the Investigation on Nutrition Status and Clinical Outcome of Patients with Common Cancers in China(INSCOC)Study:2021 update显示文摘Background Malnutrition is common in patients with cancer,and this adversely affects the survival and quality of life of patients.Chinese Society for Nutritional Oncology issued a multi-center,large-scale,long-term follow-up prospective study,the Investigation on Nutrition Status and Clinical Outcome of Patients with Common Cancers in China(INSCOC study)since 2013.This is an extension to the previous 2013-2020 study protocol.This study still sought to:①address the prognostic impact of nutritional factors and quality of life on cancer patient survival;②describe the overall and cancer-specific incidence and/or distribution of malnutrition and different measurements of patient quality of life.Methods and study design This is an observational,multi-centered,hospital-based prospective cohort study.Data collection will be performed at baseline(within 48 hours after patient admission),during the hospital stay and 30 days after hospital admission.Follow-up will be conducted for 1-20 years after enrollment.The primary outcome will be the all-cause mortality/overall survival,and secondary outcomes will be the length of hospital stay and costs of hospitalization.Study factors will include demographic characteristics,tumor characteristics,information about chronic diseases,hematological measurements(e.g.,red blood cell count,total lymphocyte counts,hemoglobin,albumin,prealbumin,creatinine,C-reactive protein,IL-6),anthropometric measurements(e.g.,height,weight,arm circumference,arm muscle circumference,triceps skinfold thickness,and waist circumference),body composition parameters,PG-SGA scores,quality of life(as indicated by the QLQ-C30 questionnaire),muscle mass(as indicated by the calf circumference),muscle strength(as indicated by the handgrip strength),muscle function(as indicated by the six-meter walking speed test)and physical status assessments(as indicated by the Karnofsky Performance Status scores).This clinical study protocol was approved by local Ethics Committees of all the participating hospitals.Written informed consent is required for each subject included.Discussion This multi-center,large-scale,long-term follow up prospective study will help improve the diagnosis of malnutrition in cancer patients and identify the risk factors associated with adverse clinical outcomes.The anticipated results of this study will highlight the need for a truly scientific appraisal of nutrition therapy in Chinese oncology populations,and finally help treat the potentially reversible elements of malnutrition in cancer patients to improve their clinical outcomes in the future. | Hong Xia Xu Chun Hua Song Liang Yu Yin Chang Wang Zhen Ming Fu Zeng Qing Guo Yuan Lin Ying Ying Shi Wen Hu Yi Ba Su Yi Li Zeng Ning Li Kun Hua Wang Jing Wu Ying He Jia Jun Yang Cong Hua Xie Fu Xiang Zhou Xin Xia Song Gong Yan Chen Wen Jun Ma Su Xia Luo Zi Hua Chen Zhi Kang Chen Ming Hua Cong Hu Ma Chun Ling Zhou Wei Wang Qi Luo Yong Mei Shi Yu Mei Qi Hai Ping Jiang Wen Xian Guan Jun Qiang Chen Xiang Hua Wu Jia Xin Chen Yu Fang Lan Zhou Yong Dong Feng Rong Shao Tan Tao Li Jun Wen Ou Qing Chuan Zhao Jian Xiong Wu Min Weng Qing Hua Yao Ya Yin Yu Quan Jun Lyu Hong Qiu Ming Liu Wei Li Han Ping Shi The Investigation on Nutrition Status and Clinical Outcome of Common Cancers(INSCOC)Group | 2022 | Journal of Nutritional Oncology2022，7，2： | 3 |
| 8 | Influence of Polluted SY River on Child Growth and Sex Hormones显示文摘Objective To investigate the influence of the polluted SY River on children's growth and sex hormones,and provide scientific data for assessment of the polluted status of the SY River.Methods The study areas were selected randomly from the SY River Basin.Lead (Pb),mercury (Hg),arsenic (As),phthalates (DEP,DBP,DMP,DEHP),and bisphenol A (BPA) were measured both in the river water and in the drinking water.School children were selected by cluster sampling (n=154).Physical development indexes (height,weight,bust-circumference,and skinfold thickness) and sex hormones [testosterone (T) and estradiol (E2)] were measured for all the children.Results The contents of Pb and Hg exceeded Class V standards of surface water quality in each section of the river and other indicators exceeded Class III.Compared to the control area,the concentrations of Pb,Hg,As,BPA,DEP,and DBP in the drinking water were significantly higher than in the polluted area (P<0.05).Children from the control area had significantly lower E2 and T than children from the polluted area (P<0.05).Among anthropometric results,only skinfold thickness had statistically significant difference between the two groups (P<0.05),while the other indexes showed no significant differences between the two groups (P>0.05).Conclusion The drinking water has been polluted by the SY River and affected serum sex hormone levels of children living in the polluted area. | TANG Chun Yu LI An Qi GUAN Yong Bo LI Yan CHENG Xue Min LI Ping LI Shi Qun LUO Yi Xin HUANG Qi CHEN Hong Yang CUI Liu Xin | 2012 | Biomedical and Environmental Sciences2012，25，3： | 2 |
| 9 | Discovery and structural optimization of 4-( 4-( benzyloxy)p henyl)-3,4-dihyd ropyrimidin-2(1H )- ones as RORc inverse agonists显示文摘Retinoic 酸受体相关的孤儿原子受体(ROR ) 是孤儿当 ligands 不在时显示出组成的活动的原子受体。在 ROR 的 3 种子类型之中, RORc 是为调停 Th17 的自体免疫的疾病的处理的一个有希望的治疗学的目标。这里,我们报导通过基于结构的药 design.Methods 发现的新奇 RORc 反的收缩筋:基于化合物 8 的结构, RORa 的以前描述的收缩筋,一系列 4-(4-(benzyloxy ) 苯基)-3,4-dihydropyrimidin-2(1H)-one 衍生物被设计并且综合。在混合物和 RORc 之间的相互作用用 AlphaScreen 试金在分子的水平被检测。混合物进一步与 RORc 和酶记者基因在 293T 房间 transfected 被检验。热稳定性移动试金被用来在蛋白质 stability.Results 上评估混合物的效果:27 衍生物的一个总数被设计并且综合。在他们之中,复合 22b 作为大多数有势力 RORc 反的收缩筋被识别。它的 IC 50 价值是 2.39 μ在 AlphaScreen 试金的 mol/L,和 0.82 μ在基于房间的酶记者活动的抑制的 mol/L。而且,复合 22b 在另外的原子受体上为 RORc 显示了一种 120 褶层选择。而且,分子的停靠研究证明结构活动关系与在 RORc.Conclusion 的复合 22b 的有约束力的模式一致:-3,4-dihydropyrimidin-2(1H)-one 衍生物为调停 Th17 的自体免疫的疾病的治疗正在答应候选人的 4-(4-(Benzyloxy ) 苯基) 例如风湿性关节炎,干癣,和多重硬化。 | Xi-shan WU Rui WANG Yan-li XING Xiao-qian XUE Yan ZHANG Yong-zhi LU Yu SONG Xiao-yu LUO Chun WU Yu-lai ZHOU Jian-qin JIANG Yong XU | 2016 | Acta Pharmacologica Sinica2016，37，11： | 1 |
| 10 | Synthesis and characterization of biodegradable polymer:Poly (ethene maleic acid ester-co-D,L-lactide acid)显示文摘新奇可被细菌破坏的聚合物—poly (ethene 马来酸 ester-co-d, l 减水乳酸酸) 被异分子聚合综合减水乳酸和 prepolymer,它被冷凝作用准备缩苹果酸酐和乙二醇,把 p 甲苯磺基酸用作催化剂,试图改进 hydrophilicity,增加灵活性并且调制降解速率。FTIR,(1 ) H NMR,广场和 DSC 被采用描绘这些聚合物。 | Mei Na Huang Yan Feng Luo Jia Chen Yong Gang Li Chun Hua Fu Yuan Liang Wang | 2007 | Chinese Chemical Letters2007，18，5： | 1 |
| 11 | The cell surface marker CD36 selectively identifies matured, mitochondria-rich hPSC-cardiomyocytes显示文摘Dear Editor,Human pluripotent stem cell(hPSC)-derived cardiomyocytes(CMs)are of significant translational value to in vitro studies of human cardiac development,drug and cardiotoxicity testing and cardiac disease modelling.Differentiation of hPSCs to CMs,however,yields mixed cultures of atrial-,ventricular-,and pacemaker-like cells as well as non-CMs in variable proportions.1 Strategies to enrich CMs from non-CMs and to generate ventricular versus atrial cells have been successful;however,enriched hPSC-CMs are developmentally immature and fail to recapitulate key functional traits that are fundamental to the(patho)physiology of adult CMs.1,2 Moreover,these strategies do not adequately address experimental variabilities caused by differences in the genomes and differentiation capabilities of diverse hPSC lines.One validated approach that overcomes issues of cell heterogeneity and experimental variability is immunophenotyping;however,accessible markers suitable for defining mature,live CMs are lacking.Although cell surface markers such as SIRPA(CD172a)and VCAM1(CD106)3,4 have been used to sort for hPSC-CMs,they do not distinguish between maturation states.Here,we report the identification of CD36 as a cell surface marker of maturation,which can be used to reduce experimental variability and improve drug screens. | Ellen Ngar-Yun Poon Xiao-ling Luo Sarah EWebb Bin Yan Rui Zhao Stanley Chun Ming Wu Yong Yang Peng Zhang Huajun Bai Jiaofang Shao Ching Man Chan Godfrey Chi-Fung Chan Suk Ying Tsang Rebekah LGundry Huang-Tian Yang Kenneth RBoheler | 2020 | Cell Research2020，30，7： | 1 |
| 12 | MEK2 is a prognostic marker and potential chemo-sensitizing target for glioma patients undergoing temozolomide treatment显示文摘尽管 temozolomide (TMZ ) 是为 glioblastoma 的首要的化学疗法的代理人,它经常由于药抵抗是非药品。克服 glioblastoma 房间的抵抗到 TMZ,为结果预言识别预示的标记并且开发敏化 chemo 代理人是必要的。这里, TMZ 抵抗、 TMZ 敏感的样品的基因表示侧面被 microarray 分析,和激活 mitogen 的蛋白质 kinase kinase 比较(MEK2 ) 2 在抵抗 glioma 房间然而并非在敏感肿瘤房间或非肿瘤纸巾明确地是 upregulated。而且,耐心的数据的全面分析表明 MEK2 表示的增加的水平响应 TMZ 治疗与 glioma 等级和更坏的预后的前进相关很好。而且,在 U251 glioma 房间线或 xenografted glioma 减少 MEK2 的水平通过调停 shRNA 的基因为击倒的禁止的房间增长建模并且向 TMZ 治疗提高了房间的敏感。从由即时 PCR 的 glioma 病人的肿瘤样品的进一步的分析显示增加的 MEK2 表示水平仔细与许多药抵抗基因的激活被联系。最后,在 MEK2 是在 vitro 的 silenced 以后,这些抵抗基因是 downregulated,建议导致 MEK2 的 chemo 抵抗的机制能被这些抵抗基因的 transcriptional 激活调停。一起,我们的数据显示 MEK2 的表示水平能为 glioma 化疗用作一个预示的标记并且 MEK2 对手能被用作 chemo-sensitizers 提高 TMZ 的治疗功效。 | Hua He Maojin Yao Wenhao Zhang Bangbao Tao Feili Liu Shu Li Yan Dong Chenran Zhang Yicheng Meng Yuxin Li Guohan Hu Chun Luo Hui Zong Yicheng Lu | 2016 | Cellular & Molecular Immunology2016，13，5： | 1 |
| 13 | The concentration of oxygen species over SiO2-supported Rh and Ru catalysts and its relationship with the mechanism of partial oxidation of methane to synthesis gas显示文摘 | Wei Zheng Weng Qian Gu Yan Chun Rong Luo Yuan Yan Liao Hui Lin Wan | 2001 | Catalysis Letters (-)2001，，1： | 1 |
| 14 | Comparative proteomic profiles indicating genetic factors may involve in hepatocellular carcinoma familial aggregation显示文摘 | Da‐Ni Zhong Qiu‐Yue Ning Ji‐Zhou Wu Ning Zang Jian‐Lin Wu Die‐Fei Hu Shuang‐Yan Luo Ai‐Chun Huang Lan‐Lan Li Guo‐Jian Li | 2012 | Cancer Sci2012，，10： | 1 |
| 15 | Environmental microcystin exposure triggers the poor prognosis of prostate cancer: Evidence from case-control, animal, and in vitro studies显示文摘Microcystin-leucine-arginine(MC-LR)is positively linked with multiple cancers in humans.However,the association between MC-LR and the risk and prognosis of prostate cancer has not been conducted in epidemiological studies.No reported studies have linked MC-LR exposure to the poor prognosis of prostate cancer by conducting experimental studies.The content of MC-LR was detected in most of the aquatic food in wet markets and supermarkets in Nanjing and posed a health risk for consumers.MC-LR levels in both prostate cancer tissues and serum were significantly higher than controls.The adjusted odds ratio(OR)for prostate cancer risk by serum MC-LR was 1.75(95%CI:1.21-2.52)in the whole subjects,and a positive correlation between MC-LR and advanced tumor stage was observed.Survival curve analysis indicated patients with higher MC-LR levels in tissues exhibited poorer overall survival.Human,animal,and cell studies confirmed that MC-LR exposure increases the expression of estrogen receptor-α(ERα)and promotes epithelial-mesenchymal transition(EMT)in prostate cancer.Moreover,MC-LR-induced decreased E-cadherin levels,increased vimentin levels,and increased migratory and invasive capacities of prostate cancer cells were markedly suppressed upon ERαknockdown.MC-LR-induced xenograft tumor growth and lungmetastasis in BALB/c nude mice can be effectively alleviated with ERαknockdown.Our data demonstrated that MC-LR upregulated vimentin and downregulated E-cadherin through activating ERα,promoting migration and invasion of prostate cancer cells.Our findings highlight the role of MC-LR in prostate cancer,providing new perspectives to understand MC-LR-induced prostatic toxicity. | Chun Pan Haixiang Qin Minghao Yan Xuefeng Qiu Wenyue Gong Wenxin Luo Hongqian Guo Xiaodong Han | 2023 | Journal of Environmental Sciences2023，，5： | 1 |
| 16 | Haemorrhage risk of brain arteriovenous malformation during pregnancy and puerperium显示文摘Background This study aimed to assess whether pregnancy and puerperium were associated with the risk of brain arteriovenous malformation(bAVM)haemorrhage.Methods A retrospective review was conducted in Xiangya Hospital,Central South University from January 2012 to December 2021.A case-crossover design was adopted to calculate the incidence density of bAVM-related haemorrhage among female patients in risk(pregnancy and puerperium)and control(non-pregnancy and non-puerperium)periods,according to four scenarios observed in different populations scenario I:patients with haemorrhagic bAVM of all ages;scenario II:patients with haemorrhagic bAVM of all ages,with at least one previous pregnancy;scenario III:patients with haemorrhagic bAVM who are of reproductive age(15-45 years);scenario IV:patients with haemorrhagic bAVM of reproductive age(15-45 years),with at least one previous pregnancy.Next,a comprehensive literature aggregation(up to April 2022)was performed for evidence synthesis.Results Among the 311 female patients with haemorrhagic bAVM,a significant haemorrhage risk during pregnancy and puerperium was found in Scenarios I(relative risk[RR],2.08;95%CI,1.28 to 3.39),II(RR,3.21;95%CI,1.95 to 5.31)and IV(RR,2.92;95%CI,1.73 to 4.93);however,a suggestive risk was found in scenario III(RR,1.62;95%CI,0.99 to 2.67).Evidence synthesis revealed a consistent haemorrhage risk among patients of all ages(RR,3.15;95%CI,1.93 to 5.15)and those of reproductive age(RR,1.29;95%CI,0.89 to 1.86).Conclusion Compared with most previous studies,a higher but relatively moderate risk for bAVM-related haemorrhage was identified during pregnancy and puerperium.Individualised prevention and treatment strategies should be preferred when neurosurgeons make clinical decisions. | Junyu Liu Honghao Zhang Chun Luo Yuxin Guo Yifeng Li Dun Yuan Weixi Jiang Junxia Yan | 2023 | Stroke & Vascular Neurology2023，8，4： | 1 |
| 17 | Differential diagnosis of high-grade squamous intraepithelial lesions and benign atrophy in older women using p16 immunocytochemistry显示文摘Background For cervical cancer screening,routine cytology has a high specificity but a lower sensitivity.In older women,atrophy,which may mimic HSIL,presents a diagnostic challenge.p16 is a widely used biomarker for histological diagnosis of HSIL.Our objective was to evaluate PathCIN®p16INK4a immunocytology in identification of high grade dysplasia vs.benign atrophy.Methods As part of a multi-center screening program,3351 women were co-tested by p16 immunocytology.Among women referred for colposcopy on basis of cytology and high-risk HPV status,those with atrophy were older than the population screened(52 vs.43 years).Cases from older women with atrophy(n=116)and controls without atrophy(n=47)were identified by re-examination of Pap smears.The detection of CIN2+was compared for p16,cytology and HR-HPV results.Results The sensitivity of routine cytology(≥LSIL)was much lower for cases with atrophy(17%)than non-atrophic cases(75%).The sensitivity of p16 immunocytology and of HR-HPV testing was high(88%–100%)both with and without atrophy.The specificity of routine cytology(≥LSIL)was higher for cases with atrophy(79%)than non-atrophic cases(38%).The specificity of p16 immunocytology was high(88–95%)and the specificity of HR-HPV testing was low(31%–33%)both with and without atrophy.Combining p16 with HPV testing and/or routine cytology had no benefit,as compared to p16 staining alone.Conclusions p16 immunocytology compares favorably with routine cytology and HPV testing in the differential diagnosis of HSIL and benign atrophy.It is more sensitive than cytology for atrophic specimens,and is more specific than HPV testing.p16 immunocytology may decrease the need for colposcopy referrals and could be a useful tool for early detection of cervical cancer in peri-and post-menopausal women,who are more likely to have HSIL coexisting with atrophy. | Peisha Yan Hui Du Chun Wang Fangbin Song Xia Huang Yingjie Luo Ruifang Wu | 2021 | Gynecology and Obstetrics Clinical Medicine2021，1，1： | 0 |
| 18 | Lentiviral-mediated siRNA against osteopotin in U251 glioma cell line显示文摘Background:It has been reported that the expression of SPP1, the mRNA for osteopontin (OPN), is increased in a number of transformed cell lines and some tumors. Increased serum and plasma OPN levels were associated with advanced-stage carcinomas of the lung, breast, colon and prostate. The extent of OPN expression may be correlated with the malignancy degree of gliomas, but the specific role of OPN in the malignancy of gliomas remains to be clarified. Methods:In this study, we used a lentiviral system, which could induce a long-lasting down-regulation of gene expression, to knock down the OPN gene in human glioma cell line U251. And then we observed the proliferation and apoptosis of glioma cells. Results:OPN mRNA and protein were successfully knocked down in U251 cells, and the expression of caspase-3 and caspase-9 were increased about by 2 folds in OPN down-regulated U251 cells. 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium (MTT) assay showed that OPN down-regulation did not affect the proliferation of U251 cells. Conclusion:Our findings indicate that OPN might play a role in apoptosis, but not in cell proliferation in U251 glioma cells. | Xu Tao Chen Juxiang Lu Yicheng Zhou Jinxu Yan Yong Hu Guohan Luo Chun Huang Yan | 2010 | Journal of Medical Colleges of PLA(China)2010，25，1： | 0 |
| 19 | Comparison of Lipopolysaccharide and Protein Immunogens from Pathogenic Yersinia enterocolitica Bio-serotype 1B/O:8 and 2/O:9 using SDS-PAGE显示文摘Objective Yersinia enterocolitica is an extracellular pathogen and its related antigens interact with the host immune system.We investigated the difference in immunological characteristics between a highly pathogenic and poorly pathogenic strain of Y.enterocolitica.Methods We used SDS-PAGE and western blotting to characterize lipopolysaccharide (LPS),Yersinia outer membrane proteins (Yops),membrane proteins,and whole-cell proteins from poorly pathogenic Y.enterocolitica bio-serotype 2/O:9,isolated from China,and highly pathogenic bio-serotype 1B/O:8,isolated from Japan.Results These two strains of Y.enterocolitica had different LPS immune response patterns.Comparison of their Yops also showed differences that could have accounted for their differences in pathogenicity.The membrane and whole-cell proteins of both strains were similar;immunoblottting showed that the 35 kD and perhaps the 10 kD proteins were immunogens in both strains.Conclusion The major antigens of the two strains eliciting the host immune response were the LPS and membrane proteins,as shown by comparing protein samples with reference and purified preparations. | GU Wen Peng WANG Xin QIU Hai Yan LUO Xia XIAO Yu Chun TANG Liu Ying KAN Biao XU Jian Guo JING Huai Qi | 2012 | Biomedical and Environmental Sciences2012，25，3： | 0 |
| 20 | Applications of protein engineering in the microbial synthesis of plant triterpenoids显示文摘Triterpenoids are a class of natural products widely used in fields related to medicine and health due to their biological activities such as hepatoprotection,anti-inflammation,anti-viral,and anti-tumor.With the advancement in biotechnology,microorganisms have been used as cell factories to produce diverse natural products.Despite the significant progress that has been made in the construction of microbial cell factories for the heterogeneous biosynthesis of triterpenoids,the industrial production of triterpenoids employing microorganisms has been stymied due to the shortage of efficient enzymes as well as the low expression and low catalytic activity of heterologous proteins in microbes.Protein engineering has been demonstrated as an effective way for improving the specificity,catalytic activity,and stability of the enzyme,which can be employed to overcome these challenges.This review summarizes the current progress in the studies of Oxidosqualene cyclases(OSCs),cytochrome P450s(P450s),and UDP-glycosyltransferases(UGTs),the key enzymes in the triterpenoids synthetic pathway.The main obstacles restricting the efficient catalysis of these key enzymes are analyzed,the applications of protein engineering for the three key enzymes in the microbial synthesis of triterpenoids are systematically reviewed,and the challenges and prospects of protein engineering are also discussed. | Yan Luo Yaozhu Jiang Linhao Chen Chun Li Ying Wang | 2023 | Synthetic and Systems Biotechnology2023，8，1： | 0 |
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