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| 1 | Efficient and Specific Modifications of the Drosophila Genome by Means of an Easy TALEN Strategy显示文摘技术开发总是是在生物医学的研究驾驶突破的力量之一。自从托马斯·摩根的时间起,一步一步地, Drosophilists 为操作并且机能上地把开发了强大的基因工具为改进这些技术并且开发新技术的果蝇染色体,而是房间仍然大,今天特别作为生物学家开始系统地学习不同模型有机体的功能的 genomics ,包括人,以一种高产量的方式。这里,我们报导,第一次在果蝇,为借助于改进抄写在很高的效率修改果蝇染色体的一个快速、容易、高度特定的方法像使活跃之物的受动器核酸酶(TALEN ) 策略。我们利用了最近发达的单位集会策略装配设计修改黄基因的二特定的 TALEN (在性染色体上) 并且新奇正染色体的基因。TALEN 的 mRNAs 随后被注入果蝇胚胎。从 31.2% 注射 F0 肥沃的苍蝇,我们检测了包含黄基因的可继承的修正。从到可继承的修正的察觉的特定的 TALEN 的建设的全部过程能在一个月以内被完成。在果蝇的这个调停 TALEN 的染色体修正方法的潜在的应用程序被讨论。 | Jiyong Liu Changqing Li Zhongsheng Yu Peng Huang Honggang Wu Chuanxian Wei Nannan Zhu Yan Shen Yixu Chen Bo Zhang Wu-Min Deng Renjie Jiao | 2012 | Journal of Genetics and Genomics2012，39，5： | 43 |
| 2 | Recent Advances in Synthesis of Waterborne Polyurethane and Their Application in Water-based Ink:A Review显示文摘Over the last few years,waterborne polyurethane(WPU) was applied to various fibers,adhesives,primers for metals,caulking materials,emulsion polymerization media,paint additives,defoamers,associate thickeners,pigment pastes,textile dyes and biomaterials,resulting in the increasing enthusiasm of researchers to design and synthesize novel WPU with unique properties.Thus,various processes and raw materials have been developed to prepare WPU.This review gives an overview on the developments of WPU mainly derived from novel polyols,analyzes the potential application in water-based inks and presents the probable future research area about water-based inks.Notably,the poly(ε-caprolactone) and poly(lactic acid) are the versatile materials used in WPU synthesis and supply the potential special performance for preparing WPU.Meanwhile,addition-fragmentation chain transfer(RAFT) polymerization and atom transfer radical polymerization(ATRP) processes provide an opportunity to control the chain sequence of WPU and obtain products with the desired performance. | Xing Zhou Yan Li Changqing Fang Shujuan Li Youliang Cheng Wanqing Lei Xiangjie Meng | 2015 | Journal of Materials Science & Technology2015，31，7： | 47 |
| 3 | CRISPR-Cas9-mediated multiplex gene editing in CAR-T cells显示文摘 | Xiaojuan Liu Yongping Zhang Chen Cheng Albert W Cheng Xingying Zhang Na Li Changqing Xia Xiaofei Wei Xiang Liu Haoyi Wang | 2017 | Cell Research2017，27，1： | 40 |
| 4 | The distribution rule and seepage effect of the fractures in the ultra-low permeability sandstone reservoir in east Gansu Province,Ordos Basin显示文摘To study the impact of the fractures on development in the ultra-low permeability sandstone reservoir of the Yangchang Formation of the Upper Triassic in the Ordos Basin,data on outcrops,cores,slices,well logging and experiments are utilized to analyze the cause of the formation of the fractures,their distribution rules and the control factors and discuss the seepage flow effect of the fractures. In the studied area developed chiefly high-angle tectonic fractures and horizontal bedding fractures,inter-granular fractures and grain boundary fractures as well. Grain boundary fractures and intragranular fractures serve as vital channels linking intragranular pores and intergranular solution pores in the reservoir matrix,thus providing a good connectivity between the pores in the ultra-low perme-ability sandstone reservoir. The formation of fractures and their distribution are influenced by such external and internal factors as the palaeo-tectonic stress field,the reservoir lithological character,the thickness of the rock layer and the anisotropy of a layer. The present-day stress field influences the preservative state of fractures and their seepage flow effect. Under the tec-tonic effect of both the Yanshan and Himalayan periods,in this region four sets of fractures are distributed,respectively assuming the NE-SW,NW-SE,nearly E-W and nearly S-N orientations,but,due to the effect of the rock anisotropy of the rock formation,in some part of it two groups of nearly orthogonal fractures are chiefly distributed. Under the effect of the present-day stress field,the fractures that assume the NE-SW direction have a good connectivity,big apertures,a high permeability and a minimum starting pressure,all of which are main advantages of the seepage fractures in this region. With the development of oilfields,the permeability of the fractures of dif-ferent directions will have a dynamic change. | ZENG LianBo1,GAO ChunYu2,QI JiaFu1,WANG YongKang2,LI Liang2 & QU XueFeng2 1 State Key Laboratory of Petroleum Resource and Prospecting,China University of Petroleum,Beijing 102249,China 2 Changqing Oilfield Branch,PetroChina,Xi’an 163517,China | 2008 | Science China Earth Sciences2008，51，S2： | 22 |
| 5 | Nanomaterials Applied in Asphalt Modification:A Review显示文摘Nanotechnology has been gradually penetrated into the field of asphalt modification.Seemingly magic effects of nanomaterials have now been brought to improve the performance of asphalt.To demonstrate many of the prospective applications,researchers have conducted a series of positive and effective efforts dealing with the preparation of modified asphalt to demonstrate the mechanism of modification and the resultant improvement in performance.In this review,various nanomaterials used in asphalt modification are initially presented,followed by the methods employed to modify the asphalt with these materials and finally the effects of nanomaterials on the performance of base asphalt are presented and the modification mechanisms are discussed.Based on the current research results,the influence of preparation process parameters on the compatibility of every phase in the modified asphalt and the stability of the modified asphalt system are described.Finally,the development trend of the topic field is projected. | Changqing Fang Ruien Yu Shaolong Liu Yan Li | 2013 | Journal of Materials Science & Technology2013，29，7： | 23 |
| 6 | Recent advances in biosynthesis of bioactive compounds in traditional Chinese medicinal plants显示文摘Plants synthesize and accumulate large amount of specialized(or secondary) metabolites also known as natural products, which provide a rich source for modern pharmacy. In China, plants have been used in traditional medicine for thousands of years. Recent development of molecular biology, genomics and functional genomics as well as high-throughput analytical chemical technologies has greatly promoted the research on medicinal plants. In this article, we review recent advances in the elucidation of biosynthesis of specialized metabolites in medicinal plants,including phenylpropanoids, terpenoids and alkaloids. These natural products may share a common upstream pathway to form a limited numbers of common precursors, but are characteristic in distinct modifications leading to highly variable structures. Although this review is focused on traditional Chinese medicine, other plants with a great medicinal interest or potential are also discussed. Understanding of their biosynthesis processes is critical for producing these highly value molecules at large scale and low cost in microbes and will benefit to not only human health but also plant resource conservation. | Lei Yang Changqing Yang Chenyi Li Qing Zhao Ling Liu Xin Fang Xiao-Ya Chen | 2016 | Science Bulletin2016，61，1： | 23 |
| 7 | Special Section:SARS-CoV-2 Preliminary evidence from a multicenter prospective observational study of the safety and efficacy of chloroquine for the treatment of COVID-19显示文摘Effective therapies are urgently needed for the SARS-CoV-2 pandemic.Chloroquine has been proved to have antiviral effect against coronavirus in vitro.In this study,we aimed to assess the efficacy and safety of chloroquine with different doses in COVID-19.In this multicenter prospective observational study,we enrolled patients older than 18 years old with confirmed SARS-CoV-2 infection excluding critical cases from 12 hospitals in Guangdong and Hubei Provinces.Eligible patients received chloroquinephosphate s00 mg,orally,once(half dose)or twice(full dose)daily.Patients treated with non-chloroquine therapy were included as historical controls.The primary endpoint is the time to undetectable viral RNA.Secondary outcomes include the proportion of patients with undetectable viral RNA by day 10 and 14,hospitalization time,duration of fever,and adverse events.A total of 197 patients completed chloroquine treatment,and 176patients were included as historical controls.The median time to achieve an undetectable viral RNA was shorter in chloroquine than in non-chloroquine(absolute difference in medians-6.0 days;95%CI-6.0 to—4.0).The duration of fever is shorter in chloroquine(geometric mean ratio 0.6;95%CIO.5 to 0.8).No serious adverse events were observed in the chloroquine group.Patients treated with half dose experienced lower rate of adverse events than with full dose.Although randomized trials are needed for further evaluation,this study provides evidence for safety and efficacy of chloroquine in COVID-19 and suggests that chloroquine can be a cost-effective therapy for combating the COVID-19 pandemic. | Mingxing Huang Man Li Fei Xiao Pengfei Pang Jiabi Liang Tiantian Tang Shaoxuan Liu Binghui Chen Jingxian Shu Yingying You Yang Li Meiwen Tang Jianhui Zhou Guanmin Jiang Jingfen Xiang Wenxin Hong Songmei He Zhaoqin Wang Jianhua Feng Changqing Lin Yinong Ye Zhilong Wu Yaocai Li Bei Zhong Ruilin Sun Zhongsi Hong Jing Liu Huili Chen Xiaohua Wang Zhonghe Li Duanqing Pei Lin Tian Jinyu Xia Shanping Jiang Nanshan Zhong Hong Shan | 2020 | National Science Review2020，7，9： | 20 |
| 8 | Hypoxia-inducible factor-lαand semaphorin4D genes involved with tumor-associated macrophage-induced metastatic behavior and clinical significance in colon cancer显示文摘 | Mu Linjun Wang Jinshen Chen Yuezhi Li Leping Guo Xiaobo Zheng Sheng Jing Changqing | 2014 | Chinese Medical Journal2014，，20： | 15 |
| 9 | Effect of acupotomy on nitric oxide synthase and beta-endorphin in third lumbar vertebrae transverse process syndrome model rats显示文摘OBJECTIVE: To explore the long-term effects and pain relief mechanism of acupotomy by observing changes in nitric oxide synthase(NOS) and beta-endorphin(β-EP) in the hypothalamus, spinal cord,and peripheral blood of rats with third lumbar vertebrae(L3) transverse process syndrome.METHODS:Twenty-eight SD rats were randomly assigned to normal, model, electroacupuncture(EA),and acupotomy group. The last three groups were put through an operation to emulate L3transverse process syndrome. Fourteen days after the simulation operation, EA and acupotomy treatments were applied to the respective groups. Fifty-six days afterthe simulation operation, biochemistry tests and enzyme-linked immunosorbent assay were used to measure NOS and β-EP in the hypothalamus, spinal cord, and peripheral blood.RESULTS: Rats with the simulation operation showed significantly higher levels of NOS and β-EP in the hypothalamus, spinal cord, and peripheral blood than those in the normal group. The EA and acupotomy groups had significantly lower levels of NOS and β-EP than those in the model group.There was no statistical difference between the EA and acupotomy groups.CONCLUSION: EA and acupotomy treatments significantly lowered NOS and β-EP levels in the hypothalamus, spinal cord, and peripheral blood and alleviated L3transverse process syndrome. | Changqing Guo Naigang Liu Xiaohong Li Hongmei Sun Bo Hu Jing Lu Yan Guo Chuxi Liang Hong Xu Haixia Wu | 2014 | Journal of Traditional Chinese Medicine2014，34，2： | 15 |
| 10 | DNA image cytometry test for primary screening of esophageal cancer: a population-based multi-center study in high-risk areas in China显示文摘Objective: To evaluate the feasibility of DNA image cytometry(DNA-ICM) as a primary screening method for esophageal squamous cell cancer(ESCC).Methods: A total of 5,382 local residents aged 40–69 years from three high-risk areas in China(Linzhou in Henan province, Feicheng in Shandong province and Cixian in Hebei province) from 2008 to 2011 were recruited in this population-based screening study. And 2,526 subjects declined to receive endoscopic biopsy examination with Lugol's iodine staining, while 9 and 815 subjects were excluded from liquid-based cytology and DNA-ICM test respectively due to slide quality. Finally, 2,856, 5,373 and 4,567 subjects were enrolled in the analysis for endoscopic biopsy examination, liquid-based cytology and DNA-ICM test, respectively. Sensitivity(SE), specificity(SP), negative predictive values(NPV) and positive predictive values(PPV) as well as their 95% confidence intervals(95% CI) for DNA-ICM, liquid-based cytology and the combination of the two methods were calculated. Receiver operating characteristic(ROC) curves were applied to determine the cutoff point of DNA-ICM for esophageal cancer.Results: DNA-ICM results were significantly correlative with esophageal cancer and precancer lesions(χ~2=18.016, P<0.001). The cutoff points were 5,802, 5,803 and 8,002 based on dissimilar pathological types of low grade intraepithelial neoplasia(LGIN), high grade intraepithelial neoplasia(HGIN), and ESCC, respectively, and 5,803 was chosen in this study considering the SE and SP. The SE, SP, PPV, NPV of DNA-ICM test(cutoff point 5,803) combined with liquid-based cytology [threshold atypical squamous cells of undetermined significance(ASCUS)] were separately 72.1%(95% CI: 70.3%-73.9%), 43.3%(95% CI: 41.3%-45.3%), 22.8%(95% CI: 21.1%-24.5%) and 87.0%(95% CI: 85.7%-88.3%) for LGIN, 85.7%(95% CI: 84.3%-87.1%), 41.3%(95% CI: 39.3%-43.3%), 4.6%(95% CI: 3.8%-5.4%) and 98.9%(95% CI: 98.5%-99.3%) for HGIN, and 96.0%(95% CI: 95.2%-96.8%), 40.8%(95% CI: 38.8%-42.8%), 1.7%(95% CI: 1.2%-2.2%) and 99.9%(95% CI: 99.8%-100.0%) for ESCC.Conclusions: It is possible to use DNA-ICM test as a primary screening method before endoscopic screening for esophageal cancer. | Meng Wang Changqing Hao Qing Ma Guohui Song Shanrui Ma Deli Zhao Lin Zhao Xinqing Li Wenqiang Wei | 2016 | Chinese Journal of Cancer Research2016，28，4： | 15 |
| 11 | Family control,institutional environment and cash dividend policy:Evidence from China显示文摘Using a sample of 1486 Chinese A-share listed companies for the period 2004-2008,this study empirically tests the impact of family control,institutional environment and their interaction on the cash dividend policy of listed companies.Our results indicate that(1)family firms have a lower cash dividend payout ratio and propensity to pay dividends than non-family firms;(2) a favorable regional institutional environment has a significant positive impact on the cash dividend payout ratio and propensity to pay dividends of listed companies;and(3) the impact of the regional institutional environment on cash dividends is stronger in family firms than in non-family firms.Somewhat surprisingly,we find that controlling family shareholders in China may intensify Agency Problem Ⅰ(the owner-manager conflict) rather than Agency Problem Ⅱ(the controlling shareholder-minority shareholder conflict),and thus have a significant negative impact on cash dividend policy.In contrast,a favorable regional institutional environment plays a positive corporate governance role in mitigating Agency Problem 1 and encouraging family firms to pay cash dividends. | Zhihua Wei Shinong Wu Changqing Li Wei Chen | 2011 | China Journal of Accounting Research2011，，Z1： | 15 |
| 12 | Distribution and source of microplastics in China's second largest reservoir-Danjiangkou Reservoir显示文摘Fresh water microplastic pollution is of pressing concern globally,but its distribution and sources in reservoirs are poorly documented.Danjiangkou Reservoir is the second largest reservoir in China and is divided into the Han Reservoir and Dan Reservoir.In this work,microplastic abundances and morphological characteristics of the reservoir were investigated.The microplastic abundance of 15 main tributaries of the reservoir was also measured.The vertical distribution(in water column and sediment),horizontal distribution(in Han Reservoir and Dan Reservoir)and source of microplastics were analyzed.Microplastics accumulated in the middle layer of the reservoir,and the size and color of the microplastic particles changed from the surface to the bottom,which implies that surveys of surface water are not enough to determine the microplastic contamination for deep water reservoirs.In the surface water,the microplastic abundance in the Han Reservoir was lower than that in the Dan Reservoir(p<0.05),but microplastic abundance did not differ significantly in the intermediate and bottom water.Tributaries were one of the main sources of microplastics for Han Reservoir but not for Dan Reservoir.Agricultural cultivation in the hydro-fluctuation belt might be an important source of microplastics in the Dan Reservoir,which should be given additional attention.The results of this study can provide valuable information for developing microplastic sampling strategies in deep water reservoirs.Further studies are recommended to investigate the process through which microplastics in the hydro-fluctuation belt enter the reservoir and the sinking behavior of microplastics in the reservoir. | Li Lin Xiong Pan Sheng Zhang Danwen Li Wenliang Zhai Zhen Wang Jingxiang Tao Changqing Mi Qingyun Li John C.Crittenden | 2021 | Journal of Environmental Sciences2021，33，4： | 13 |
| 13 | Cropland redistribution to marginal lands undermines environmental sustainability显示文摘Cropland redistribution to marginal land has been reported worldwide;however,the resulting impacts on environmental sustainability have not been investigated sufficiently.Here we investigated the environmental impacts of cropland redistribution in China.As a result of urbanization-induced loss of high-quality croplands in south China(~8.5 t ha^(-1)),croplands expanded to marginal lands in northeast(~4.5 t ha^(-1)) and northwest China(~2.9 t ha^(-1)) during 1990-2015 to pursue food security.However,the reclamation in these low-yield and ecologically vulnerable zones considerably undermined local environmental sustainability,for example increasing wind erosion(+3.47%),irrigation water consumption(+34.42%),fertilizer use(+20.02%) and decreasing natural habitats(-3.11%).Forecasts show that further reclamation in marginal lands per current policies would exacerbate environmental costs by 2050.The future cropland security risk will be remarkably intensified because of the conflict between food production and environmental sustainability.Our research suggests that globally emerging reclamation of marginal lands should be restricted and crop yield boost should be encouraged for both food security and environmental benefits. | Wenhui Kuang Jiyuan Liu Hanqin Tian Hao Shi Jinwei Dong Changqing Song Xiaoyong Li Guoming Du Yali Hou Dengsheng Lu Wenfeng Chi Tao Pan Shuwen Zhang Rafiq Hamdi Zherui Yin Huimin Yan Changzhen Yan Shixin Wu Rendong Li Jiuchun Yang Yinyin Dou Wenbin Wu Liqiao Liang Bao Xiang Shiqi Yang | 2022 | National Science Review2022，9，1： | 13 |
| 14 | Mesozoic Large-scale Mineralization and Multiple Lithospheric Extensions in South China显示文摘华南是最重要的 polymetallic (钨,锡,铋,铜,银,锑,水银,稀罕金属,重稀土元素元素,黄金和铅锌) 在中国的省。这篇论文在华南描述中生代 large-scalemineralization 的基本特征。大规模矿化作用主要发生在三个 intervals:170-150 妈, 140-126 妈和 110-80 妈。在这些之中,第一个阶段被铜,铅锌和钨矿化作用和第三个阶段主要标记被锡,黄金,银和铀矿化作用主要描绘。钨和听矿化作用主要描绘的 140-126 妈的阶段是从第一的过渡间隔到第三个阶段。考虑到地区性的构造进化的当前的研究结果,在三个阶段的 large-scalemineralization 与有关,这被建议在华南块和北方中国块之间碰撞以后,到 E-Wdirection 的从 N-S 的构造政体的主要压力地的转移,并且球的延期由 Paleo-Pacificplate 的 subductlon 引起了的多重背弧石版印刷。 | MAO Jingwen XIE Guiqing LI Xiaofeng ZHANG Changqing WANG Yitian | 2006 | Acta Geologica Sinica(English Edition)2006，80，3： | 13 |
| 15 | Spatial and temporal variation of runoff of Red River Basin in Yunnan显示文摘这篇论文学习红河盆的流量的变化并且讨论“走廊障碍”的影响由基于每月的降水使用 GIS 和统计方法的流量的变化上的山谷和山的功能。 | LI Yungang HE Daming YE Changqing | 2008 | Journal of Geographical Sciences2008，18，3： | 12 |
| 16 | Buyang Huanwu Decoction fraction protects against cerebral ischemia/reperfusion injury by attenuating the inflammatory response and cellular apoptosis显示文摘Buyang Huanwu Decoction fraction extracted from Buyang Huanwu Decoction contains saponins of Astragalus, total paeony glycoside and safflower flavones. The aim of this study was to demonstrate the neuroprotective effect and mechanism of Buyang Huanwu Decoction fraction on ischemic injury both in vivo and in vitro. In vivo experiments showed that 50-200 mg/kg Buyang Huanwu Decoction fraction reduced infarct volume and pathological injury in ischemia/reperfusion rats, markedly inhibited expression of nuclear factor-κB and tumor necrosis factor-α and promoted nestin protein expression in brain tissue. Buyang Huanwu Decoction fraction (200 mg/kg) exhibited significant effects, which were similar to those of 100 mg/kg Ginkgo biloba extract. In vitro experimental results demonstrated that 10-100 mg/L Buyang Huanwu Decoction fraction significantly improved cell viability, decreased the release of lactate dehydrogenase and malondialdehyde levels, and inhibited the rate of apoptosis in HT22 cells following oxygen-glucose deprivation. Buyang Huanwu Decoction fraction (100 mg/L) exhibited significant effects, which were similar to those of 100 mg/L Ginkgo biloba extract. These findings suggest that Buyang Huanwu Decoction fraction may represent a novel, protective strategy against cerebral ischemia/reperfusion injury in rats and oxygen-glucose deprivation-induced damage in HT22 cells in vitro by attenuating the inflammatory response and cellular apoptosis. | Yulian Jin Liuyi Dong Changqing Wu Jiang Qin Sheng Li Chunyan Wang Xu Shao Dake Huang | 2013 | Neural Regeneration Research2013，8，3： | 11 |
| 17 | Transcriptional analysis and histochemistry reveal that hypersensitive cell death and H2O2 have crucial roles in the resistance of tea plant (Camellia sinensis (L.) O. Kuntze) to anthracnose显示文摘Anthracnose causes severe losses of tea production in China.Although genes and biological processes involved in anthracnose resistance have been reported in other plants,the molecular response to anthracnose in tea plant is unknown.We used the susceptible tea cultivar Longjing 43 and the resistant cultivar Zhongcha 108 as materials and compared transcriptome changes in the leaves of both cultivars following Colletotrichum fructicola inoculation.In all,9015 and 8624 genes were differentially expressed between the resistant and susceptible cultivars and their controls(0 h),respectively.In both cultivars,the differentially expressed genes(DEGs)were enriched in 215 pathways,including responses to sugar metabolism,phytohormones,reactive oxygen species(ROS),biotic stimuli and signalling,transmembrane transporter activity,protease activity and signalling receptor activity,but DEG expression levels were higher in Zhongcha 108 than in Longjing 43.Moreover,functional enrichment analysis of the DEGs showed that hydrogen peroxide(H2O2)metabolism,cell death,secondary metabolism,and carbohydrate metabolism are involved in the defence of Zhongcha 108,and 88 key genes were identified.Protein–protein interaction(PPI)network demonstrated that putative mitogen-activated protein kinase(MAPK)cascades are activated by resistance(R)genes and mediate downstream defence responses.Histochemical analysis subsequently validated the strong hypersensitive response(HR)and H2O2 accumulation that occurred around the hyphal infection sites in Zhongcha 108.Overall,our results indicate that the HR and H2O2 are critical mechanisms in tea plant defence against anthracnose and may be activated by R genes via MAPK cascades. | Yuchun Wang Xinyuan Hao Qinhua Lu Lu Wang Wenjun Qian Nana Li Changqing Ding Xinchao Wang Yajun Yang | 2018 | Horticulture Research2018，5，1： | 11 |
| 18 | Advanced Data Collection and Analysis in Data‑Driven Manufacturing Process显示文摘The rapidly increasing demand and complexity of manufacturing process potentiates the usage of manufacturing data with the highest priority to achieve precise analyze and control,rather than using simplified physical models and human expertise.In the era of data-driven manufacturing,the explosion of data amount revolutionized how data is collected and analyzed.This paper overviews the advance of technologies developed for in-process manufacturing data collection and analysis.It can be concluded that groundbreaking sensoring technology to facilitate direct measurement is one important leading trend for advanced data collection,due to the complexity and uncertainty during indirect measurement.On the other hand,physical model-based data analysis contains inevitable simplifications and sometimes ill-posed solutions due to the limited capacity of describing complex manufacturing process.Machine learning,especially deep learning approach has great potential for making better decisions to automate the process when fed with abundant data,while trending data-driven manufacturing approaches succeeded by using limited data to achieve similar or even better decisions.And these trends can demonstrated be by analyzing some typical applications of manufacturing process. | Ke Xu Yingguang Li Changqing Liu Xu Liu Xiaozhong Hao James Gao Paul G.Maropoulos | 2020 | Chinese Journal of Mechanical Engineering2020，33，3： | 9 |
| 19 | Estimation of herbage intake and digestibility of grazing sheep in Zhenglan Banner of Inner Mongolia by using n-alkanes显示文摘To reveal the seasonal dynamics of herbage intake, diet composition and digestibility and clarify the relationship of those with herbage nutrient and botanical composition of grazing sheep in Zhenglan Banner of Inner Mongolia, the n-alkane technique was used to test in sheep grazed during June, August and December. The results showed that the sheep mainly ate Fringed sagebrush, Stipa krylovii and Carex in proportions of 33.5,17.9 and 21.2%, respectively, in spring. In summer, the sheep consumed cleistogenes,Potentilla tanacetifolia, Thyme, etc; the intake of Fringed sagebrush, Carex and Stipa declined. In winter,Fringed sagebrush accounted for 50.1% of herbage intake, and the intakes of Cleistogenes and Stipa krylovii increased to 15.3 and 18.4%, respectively. Herbage intake by the sheep in spring was 1.8 kg DM/d, and digestibility was 71.4%. Herbage intake and digestibility decreased slightly to 1.7 kg DM/d and 68.4%during the summer, respectively and decreased significantly to 1.2 kg DM/d and 36.4% in winter. There were significant correlations between diet composition and CP content in winter, diet composition and botanical composition in summer. A highly positive correlation between herbage intake and digestibility was observed in grazing sheep. | Changqing Li Alatengdalai Shuyuan Xue Atsushi Tajima Naoto Ishikawa | 2015 | Animal Nutrition2015，，4： | 9 |
| 20 | Expression of human cytomegalovirus components in the brain tissues of patients with Rasmussen's encephalitis显示文摘Rasmussen's encephalitis(RE) is a rare and severe progressive epileptic syndrome with unknown etiology. Infection by viruses, including human cytomegalovirus(HCMV), has been speculated to be a potential trigger for RE. However, no viral antigens have been detected in the brains of patients with RE; thus, a possible clinical linkage between viral infections and RE has not been firmly established. In this study, we evaluated the expression of HCMV pp65 antigen in brain sections from 26 patients with RE and 20 non-RE patients by immunohistochemistry and in situ hybridization, and assessed the associations between HCMV infection and clinical parameters.Elevated expression of HCMV pp65 protein and DNA was observed in 88.5%(23/26) and 69.2%(18/26) of RE cases, respectively. In the non-RE group, HCMV pp65 antigen was detected only in two cases(10%), both of which were negative for DNA staining. Additionally, the intensity of HCMV pp65 staining was correlated with a shorter duration of the prodromal stage, younger age of seizure onset, and more severe unilateral cortical atrophy. Elevated expression of HCMV pp65 was observed in RE brain tissue and was correlated with the clinical features of RE disease. In summary, our results suggested that HCMV infection may be involved in the occurrence and progression of RE disease. Thus, further studies are needed to determine whether early treatment with anti-HCMV antibodies could modulate the course of RE. | Yao Zhang Yisong Wang Sichang Chen Shuai Chen Yuguang Guan Changqing Liu Tianfu Li Guoming Luan Jing An | 2017 | Virologica Sinica2017，32，2： | 9 |
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