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| 1 | Rice Functional Genomics Research: Past Decade and Future显示文摘瑞斯(Oryza sativa ) 是为世界范围的超过 35 亿个人的主要主食食物庄稼。在米饭理解复杂农学的特点的规章的机制为全球食物安全是批评的。瑞斯也是为单子叶植物的 genomics 研究的模型植物。由于功能的 genomic 技术的快速的发展,控制重要农学的特点的超过 2000 基因被克隆,并且他们的分子的生物机制也是部分描绘了。这里,我们简短在米饭考察进展功能的 genomics 研究在过去的 10 年期间包括功能的 genomics 平台的一篇摘要,基因 ? 并且调整重要农学的特点的分子的规章的网络,并且 ? 为基因鉴定的最新发达的工具。在功能的 genomics 研究做的这些成就将极大地便于发展 ? 绿超级米饭。我们也讨论未来挑战和米饭的前景功能的 genomics 研究。 | Yan Li Jinghua Xiaot Lingling Chen Xuehui Huang Zhukuan Cheng Bin Han Qifa Zhang Changyin Wu | 2018 | Molecular Plant2018，11，3： | 14 |
| 2 | Kinetic and thermodynamic studies of the esterification of acidified oil catalyzed by sulfonated cation exchange resin显示文摘This study describes the kinetics and thermodynamics of the esterification of acidified oil with methanol catalyzed by sulfonated cation exchange resins(SCER). The effects of the mass ratio of methanol to acidified oil,reaction temperature,and catalyst loading were studied to optimize the conditions for maximum conversion of free fatty acids(FFAs). The results showed that the optimal conversion rate of FFAs was 91.87% at the mass ratio of methanol to acidified oil of 2.5:1.0,reaction temperature of 65.0 °C,catalyst loading of 5.0 g and reaction time of 8.0 h. The external and internal mass transfer resistances were negligible based on the experimental results and a pseudo-homogeneous kinetic model was proposed for the esterification. The activation energy and thermodynamic parameters including G,S and H were determined. The conversion rates of FFAs obtained from the established model were in good agreement with the experimental data. | Lingling Ma Ying Han Kaian Sun Jie Lu Jincheng Ding | 2015 | Journal of Energy Chemistry2015，24，4： | 8 |
| 3 | A structural and data-driven approach to engineering a plant cytochrome P450 enzyme显示文摘Functional manipulation of biosynthetic enzymes such as cytochrome P450 s(or P450 s) has attracted great interest in metabolic engineering of plant natural products.Cucurbitacins and mogrosides are plant triterpenoids that share the same backbone but display contrasting bioactivities.This structural and functional diversity of the two metabolites can be manipulated by engineering P450 s.However,the functional redesign of P450 s through directed evolution(DE) or structure-guided protein engineering is time consuming and challenging,often because of a lack of high-throughput screening methods and crystal structures of P450 s.In this study,we used an integrated approach combining computational protein design,evolutionary information,and experimental data-driven optimization to alter the substrate specificity of a multifunctional P450(CYP87 D20)from cucumber.After three rounds of iterative design and evaluation of 96 protein variants,CYP87 D20,which is involved in the cucurbitacin C biosynthetic pathway,was successfully transformed into a P450 mono-oxygenase that performs a single specific hydroxylation at C11 of cucurbitadienol.This integrated P450-engineering approach can be further applied to create a de novo pathway to produce mogrol,the precursor of the natural sweetener mogroside,or to alter the structural diversity of plant triterpenoids by functionally manipulating other P450 s. | Dawei Li Yongshuo Ma Yuan Zhou Junbo Gou Yang Zhong Lingling Zhao Lida Han Sergey Ovchinnikov Ling Ma Sanwen Huang Per Greisen Yi Shang | 2019 | Science China(Life Sciences)2019，62，7： | 5 |
| 4 | The heterogeneity of islet autoantibodies and the progression of islet failure in type 1 diabetic patients显示文摘Type 1 diabetes mellitus is heterogeneous in many facets. The patients suffered from type 1 diabetes present several levels of islet function as well as variable number and type of islet-specific autoantibodies. This study was to investigate prevalence and heterogeneity of the islet autoantibodies and clinical phenotypes of type 1 diabetes mellitus; and also discussed the process of islet failure and its risk factors in Chinese type 1 diabetic patients. A total of 1,291 type 1 diabetic patients were enrolled in this study. Demographic information was collected. Laboratory tests including mixed-meal tolerance test, human leukocyte antigen alleles, hemoglobin A1 c, lipids, thyroid function and islet autoantibodies were conducted. The frequency of islet-specific autoantibody in newly diagnosed T1 DM patients(duration shorter than half year) was 73% in East China. According to binary logistic regressions, autoantibody positivity, longer duration and lower Body Mass Index were the risk factors of islet failure. As the disease developed, autoantibodies against glutamic acid decarboxylase declined as well as the other two autoantibodies against zinc transporter 8 and islet antigen 2. The decrease of autoantibodies was positively correlated with aggressive beta cell destruction. Autoantibodies can facilitate the identification of classic T1 DM from other subtypes and predict the progression of islet failure. As there were obvious heterogeneity in autoantibodies and clinical manifestation in different phenotypes of the disease, we should take more factors into consideration when identifying type 1 diabetes mellitus. | Jin Liu Lingling Bian Li Ji Yang Chen Heng Chen Yong Gu Bingqin Ma Wei Gu Xinyu Xu Yun Shi Jian Wang Dalong Zhu Zilin Sun Jianhua Ma Hui Jin Xing Shi Heng Miao Bing Xin Yan Zhu Zhenwen Zhang Ruifang Bu Lan Xu Guangde Shi Wei Tang Wei Li Dongmei Zhou Jun Liang Xingbo Cheng Bimin Shi Jixiang Dong Ji Hu Chen Fang Shao Zhong Weinan Yu Weiping Lu Chenguang Wu Li Qian Jiancheng Yu Jialin Gao Xiaoqiang Fei Qingqing Zhang Xueqin Wang Shiwei Cui Jinluo Cheng Ning Xu Guofeng Wang Guoqing Han Chunrong Xu Yun Xie Minmin An Wei Zhang Zhixiao Wang Yun Cai Qi Fu Yu Fu Shuai Zheng Fan Yang Qingfang Hu Hao Dai Yu Jin Zheng Zhang Kuanfeng Xu Yifan Li Jie Shen Hongwen Zhou Wei He Xuqin Zheng Xiao Han Liping Yu Jinxiong She Mei Zhang Tao Yang | 2016 | Science China(Life Sciences)2016，59，9： | 5 |
| 5 | Spatial-temporal Evolution Characteristics and Decoupling Analysis of Influencing Factors of China’s Aviation Carbon Emissions显示文摘The aviation industry has become one of the top ten greenhouse gas emission industries in the world. China’s aviation carbon emissions continue to increase, but the analysis of its influencing factors at the provincial level is still incomplete. This paper firstly uses Stochastic Impacts by Regression on Population, Affluence and Technology model(STIRPAT) model to analyze the time series evolution of China’s aviation carbon emissions from 2000 to 2019. Secondly, it uses the Logarithmic Mean Divisia Index(LDMI) model to analyze the influencing characteristics and degree of four factors on China’s aviation carbon emissions, which are air transportation revenue, aviation route structure, air transportation intensity and aviation energy intensity. Thirdly, it determines the various factors’ influencing direction and evolution trend of 31 provinces’ aviation carbon emissions in China(not including Hong Kong, Macao, Taiwan of China due to incomplete data). Finally, it derives the decoupling effort model and analyzes the decoupling relationship and decoupling effort degree between air carbon emissions and air transportation revenue in different provinces. The study found that from 2000 to2019, China’s total aviation carbon emissions continued to grow, while the growth rate of aviation carbon emissions showed a fluctuating downward trend. Air transportation revenue and aviation route structure promote the growth of total aviation carbon emissions, and air transportation intensity and aviation energy intensity have a restraining effect on the growth of total aviation carbon emissions. The scope of negative driving effect of air transportation revenue and air transportation intensity on total aviation carbon emissions in various provinces has increased. While the scope of positive driving influence of aviation route structure on total aviation carbon emissions of various provinces has increased, aviation energy intensity mainly has negative driving influence on total aviation carbon emissions of each province. Overall, the emission reduction trend in the areas to the west and north of the Qinling-Huaihe River Line is obvious. The decoupling mode between air carbon emissions and air transportation revenue in 31 provinces is mainly expansion negative decoupling.The air transportation intensity effect shows strong decoupling efforts in most provinces, the decoupling effort of aviation route structure effect and aviation energy intensity effect is not prominent. | HAN Ruiling LI Lingling ZHANG Xiaoyan LU Zi ZHU Shaohua | 2022 | Chinese Geographical Science2022，32，2： | 5 |
| 6 | Loss of Wip1 aggravates brain injury after ischaemia/reperfusion by overactivating microglia显示文摘Background and purpose The inflammatory response mediated by microglia/macrophages is closely related to cerebral ischaemia/reperfusion injury.Wild-type p53-induced protein phosphatase 1(Wip1),a serine/threonine phosphatase,is expressed in various tissues.A growing number of reports have suggested that Wip1 is a negative regulator of inflammation in peripheral tissue;however,its role in the central nervous system(CNS)remains unclear.This study aimed to clarify whether Wip1 can inhibit CNS inflammation by regulating microglia/macrophage functions after ischaemic injury.Methods A model of middle cerebral artery occlusion and reperfusion was established in mice.CNS inflammation was simulated by lipopolysaccharide treatment of primary microglia.Laser speckle imaging was used to monitor regional cerebral blood flow.Behavioural outcomes were assessed with a TreadScan gait analysis system.TTC staining was used to evaluate the infarct volume,and western blotting and immunofluorescence staining were applied to detect the phenotypical transformation of microglia.ELISA was performed to detect the levels of inflammatory factors.Results Wip1 expression was increased after ischaemia/reperfusion.Wip1-knockout(KO)mice displayed more severe brain injury than wild-type mice,as indicated by aggravated motor dysfunction,greater brain infarct volumes and higher expression of inflammatory cytokines(interleukin-6 and tumour necrosis factor alpha)in the brain.We also found that Wip1 depletion increased microglial/macrophage activation in both in vitro and in vivo models,which all showed activation of microglia/macrophages.Lentivirus-Ppm1d reversed the injury induced by Wip1-KO.Conclusions Our results suggest that Wip1 may inhibit neuroinflammation by inhibiting microglial/macrophage activation after brain ischaemia/reperfusion injury. | Feng Yan Xiang Cheng Ming Zhao Shenghui Gong Ying Han Liping Ding Di Wu Yumin Luo Wei Zuo Lingling Zhu Ming Fan Xunming Ji | 2021 | Stroke & Vascular Neurology2021，6，3： | 4 |
| 7 | Comparative Transcriptome Analysis Reveals Different Host Cell Responses to Acute and Persistent Foot-and-Mouth Disease Virus Infection显示文摘Foot-and-mouth disease virus(FMDV) rapidly causes cytopathic effects in susceptible cells. Incomplete viral clearance during the acute infection leads to persistent infection. The relationship between host gene expression and the persistent infection remains unclear. In this study, we analyzed the transcriptome profiles of BHK-21 cells acutely and persistently infected with FMDV to identify differences in gene expression. GO and KEGG enrichment analyses showed that the 8,378 differentially expressed genes were significantly enriched in categories including metabolism, biosynthesis, ribosome function, and endocytosis. In persistently infected BHK-21 cells, ribosome-and translation-related genes were significantly down-regulated. There were more differentially expressed immune-related genes during persistent infection than during acute infection. Two hundred and seventy-four genes were differentially expressed in both acutely and persistently infected BHK-21 cells. Among these genes, heat shock protein family B member 1(Hspb1) knockdown significantly inhibited FMDV replication. Our research provides a basis for further research to understand the mechanisms of persistent FMDV infection including the genes involved in FMDV replication. | Jiadai Li Lingling Han Yao Hao Yuncong Yuan Mingzhen Wang Xiu Xin Hailong Wang Fang Yu Congyi Zheng Chao Shen | 2020 | Virologica Sinica2020，35，1： | 4 |
| 8 | Five‑year outcomes of EVO implantable collamer lens implantation for the correction of high myopia and super high myopia显示文摘Background:To evaluate the long-term safety,efficacy,predictability,and stability of implantable collamer lens with a central hole(EVO ICL)implantation for correcting high myopia(HM)and super high myopia(SHM).Methods:This prospective study evaluated 83 eyes of 46 patients who were divided into groups based on their spherical equivalent refractive error(SE):HM group(−12 D≤SE<−6 D)and SHM group(SE<−12 D).They were followed up for 5 years after ICL implantation;assessments of uncorrected distance visual acuity(UDVA),corrected distance visual acuity(CDVA),manifest refractive error,axial length,intraocular pressure,corneal endothelial cell density,and vault were conducted,and a questionnaire was administered.ResuIts:At 5 years postoperatively,the safety indices of the HM and SHM groups were 1.03±0.10 and 1.32±0.39,and the efficacy indices were 0.83±0.25 and 0.86±0.32,respectively.In the HM group,60.47%and 79.07%of the eyes were within±0.50 D and±1.00 D of the attempted correction,while it was achieved for 22.50%and 47.50%of the eyes in the SHM group,respectively.The SE of the HM group decreased from−9.72±1.41 D preoperatively to 0.04±0.39 D 1 month postoperatively and−0.67±0.57 D 5 years postoperatively,while in the SHM group,it decreased from−15.78±3.06 D preoperatively to−0.69±0.97 D 1 month postoperatively and−1.74±1.19 D 5 years postoperatively.Conclusion:EVO ICL implantation is safe,effective,and predictable for correcting HM and SHM.CDVA improved more after surgery for SHM,but the growth of axial length still needs attention. | Xun Chen Xuanqi Wang Yilin Xu Mingrui Cheng Tian Han LingLing Niu Xiaoying Wang Xingtao Zhou | 2021 | Eye and Vision2021，8，1： | 4 |
| 9 | Tidal currents in the coastal waters east of Hainan Island in winter显示文摘Using the 25-h continuing hydrographic observations at three successive stations in February 2012 and the mooring time series of current observations from January to March 2015,the tidal currents and tidal energy fluxes in the coastal waters east of Hainan Island in the northwestern South China Sea were analyzed.The diurnal and semidiurnal(using K_(1) and M_(2) as proxies,respectively)tidal currents and associated isopycnal undulations were derived with harmonic analysis.Results show that the velocities of the diurnal and semidiurnal tides derived from the 25-h observations are comparable to those from the mooring series.The semi-major axes of the tidal ellipses were O(4-7 cm/s)for barotropic tides and O(2-4 cm/s)for baroclinic tides.The directions were in NE-SW at deeper stations to N-S at the shallowest station for the diurnal tide and from NW-SE to NE-SW for the semidiurnal tide.Both the diurnal and semidiurnal isopycnal fl uctuations reached O(5 m),O(8 m),and O(10 m)at the cross-shelf stations(H03,H04,and H05)from 35 m,45 m,to 55 m,respectively,showing insignifi cant vertical variation,and the barotropic signals were predominate.The baroclinic diurnal tide showed fi rst-mode structures at H03-05,as does the semidiurnal tide at H03.The semidiurnal tide at H04 and H05 exhibited higher-mode structures.The time series of both the alongshore and cross-shore components reveal the vertically propagation features of the baroclinic tidal phase and energy.The calculated horizontal energy fl uxes of the diurnal and semidiurnal internal tides decreased from O(0.1 W/m)at H05 to O(0.01 W/m)at H03,implying a propagation and dissipation of energy from off shore to inshore. | Min LI Lingling XIE Xiaolong ZONG Junyi LI Mingming LI Tong YAN Ronglei HAN | 2022 | Journal of Oceanology and Limnology2022，40，2： | 2 |
| 10 | Sustained local delivery of insulin for potential improvement of peri-implant bone formation in diabetes显示文摘Dental implantation is an effective standard treatment modality to restore missing teeth and maxillofacial defects. However, in diabetics there is an increased risk for implant failure due to impaired peri-implant osseous healing. Early topical insulin treat- ment was recently shown to normalize diabetic bone healing by rectifying impairments in osteoblastic activities. In this study, insulin/poly(lactic-co-glycolic acid) (PLGA) microspheres were prepared by a double-emulsion solvent evaporation method. Microspheres were then incorporated in fibrin gel to develop a local drug delivery system for diabetic patients requiring im- plant treatment. In vitro release of insulin from fibrin gel loaded with these microspheres was assessed, and sustained prolonged insulin release over 21 days ascertained. To assess the bioactivity of released insulin and determine whether slow release might improve impaired diabetic bone formation, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), alkaline phosphatase (ALP) activity, mineralized nodule formation, and ELISA (enzyme-linked immunosorbent assay) assays were performed. The insulin released from the drug delivery system stimulated cell growth in previously inhibited cells, and ameliorated the impaired bone-forming ability of human MG-63 cells under high glucose conditions. Fibrin gel loaded with insulin/PLGA microspheres shows potential for improving peri-implant bone formation in diabetic patients. | HAN Yong ZHANG XueYan E LingLing WANG DongSheng LIU HongChen | 2012 | Science China(Life Sciences)2012，55，11： | 2 |
| 11 | Epigenetic modification contributes to the expression divergence of three T a EXPA 1 homoeologs in hexaploid wheat ( T riticum aestivum )显示文摘 | Zhaorong Hu Zongfu Han Na Song Lingling Chai Yingyin Yao Huiru Peng Zhongfu Ni Qixin Sun | 2013 | New Phytol2013，，4： | 2 |
| 12 | Compressive and flexural behaviour of CFRP - repaired concrete - filled steel tubes after exposure to fire显示文摘 | TAO Zhong HAN Linhai WANG Lingling | 2007 | Journal of Constructional Steel Research2007，63，8： | 1 |
| 13 | FDG-PET and other imaging modalities for the evaluation of breast cancer recurrence and metastases: a meta-analysis显示文摘 | LingLing Pan Yuan Han XiaoGuang Sun JianJun Liu Huang Gang | 2010 | Journal of Cancer Research and Clinical Oncology2010，，7： | 1 |
| 14 | Overexpression of integrin-linked kinase correlates with malignant phenotype in non-small cell lung cancer and promotes lung cancer cell invasion and migration via regulating epithelial–mesenchymal transition (EMT)-related genes显示文摘 | Donghong Chen Yibing Zhang Xuhua Zhang Jing Li Bing Han Shuo Liu Lingling Wang Yuan Ling Shitao Mao Xiaoge Wang | 2012 | Acta Histochemica2012，，： | 1 |
| 15 | Ghrelin inhibits cell apoptosis induced by lipotoxicity in pancreatic β-cell line显示文摘 | Wei Wang Dan Zhang Hong Zhao Ying Chen Ying Liu Cuiping Cao Lingling Han Guoliang Liu | 2010 | Regulatory Peptides2010，，1： | 1 |
| 16 | DYZ1 copy number variation, Y chromosome polymorphism and early recurrent spontaneous abortion/early embryo growth arrest显示文摘 | Junhao Yan Lingling Fan Yueran Zhao Li You Laicheng Wang Han Zhao Yuan Li Zi-Jiang Chen | 2011 | European Journal of Obstetrics and Gynecology2011，，2： | 1 |
| 17 | Chemical substitution effects of elements on photoluminescence properties of YAG:Ce phosphors using orthogonal experimental design显示文摘 | Tao Han Shixiu Cao Lingling Peng Dachuan Zhu Cong Zhao Mingjing Tu Jin Zhang | 2012 | Optical Materials2012，，9： | 1 |
| 18 | Comparative phosphoproteomic analysis of BR-defective mutant reveals a key role of GhSK13 in regulating cotton fiber development显示文摘Brassinosteroid(BR), a steroid phytohormone, whose signaling transduction pathways include a series of phosphorylation and dephosphorylation events, and GSK3 s are the main negative regulator kinases. BRs have been shown to play vital roles in cotton fiber elongation. However, the underlying mechanism is still elusive. In this study, fibers of a BR-defective mutant Pagoda 1(pag1), and its corresponding wild-type(ZM24) were selected for a comparative global phosphoproteome analysis at critical developmental time points: fast-growing stage(10 days after pollination(DPA)) and secondary cell wall synthesis stage(20DPA). Based on the substrate characteristics of GSK3, 900 potential substrates were identified. Their GO and KEGG annotation results suggest that BR functions in fiber development by regulating GhSKs(GSK3s of Gossypium hirsutum L.) involved microtubule cytoskeleton organization, and pathways of glucose, sucrose and lipid metabolism. Further experimental results revealed that among the GhSK members identified, GhSK13 not only plays a role in BR signaling pathway, but also functions in developing fiber by respectively interacting with an AP2-like ethylene-responsive factor GhAP2L, a nuclear transcription factor GhDNFYB19, and a homeodomain zipper member GhHDZ5. Overall, our phosphoproteomic research advances the understanding of fiber development controlled by BR signal pathways especially through GhSKs, and also offers numbers of target proteins for improving cotton fiber quality. | Lingling Wang Han Cheng Fangjie Xiong Shuya Ma Lei Zheng Yun Song Kexuan Deng Huanhuan Wu Fuguang Li Zuoren Yang | 2020 | Science China(Life Sciences)2020，63，12： | 1 |
| 19 | Composite electron transport layer for efficient N-I-P type monolithic perovskite/silicon tandem solar cells with high open-circuit voltage显示文摘Perovskite/silicon tandem solar cells(PSTSCs) have exhibited huge technological potential for breaking the Shockley-Queisser limit of single-junction solar cells. The efficiency of P-I-N type PSTSCs has surpassed the single-junction limit, while the performance of N-I-P type PSTSCs is far below the theoretical value. Here, we developed a composite electron transport layer for N-I-P type monolithic PSTSCs with enhanced open-circuit voltage(VOC) and power conversion efficiency(PCE). Lithium chloride(Li Cl) was added into the tin oxide(SnO_(2)) precursor solution, which simultaneously passivated the defects and increased the electron injection driving force at the electron transfer layer(ETL)/perovskite interface.Eventually, we achieved monolithic PSTSCs with an efficiency of 25.42% and V_(OC) of 1.92 V, which is the highest PCE and VOCin N-I-P type perovskite/Si tandem devices. This work on interface engineering for improving the PCE of monolithic PSTSCs may bring a new hot point about perovskite-based tandem devices. | Bingbing Chen Pengyang Wang Renjie Li Ningyu Ren Yongliang Chen Wei Han Lingling Yan Qian Huang Dekun Zhang Ying Zhao Xiaodan Zhang | 2021 | Journal of Energy Chemistry2021，30，12： | 1 |
| 20 | Design and manufacturing of the antechamber for BEPCII storage rings显示文摘Antechamber which composes the arc vacuum chamber of storage rings is one of the key devices of BEPCII. It has the characteristic of various specifications, large size, complex structure, and high dimension accuracy. This paper gives a review on the structure features and the procedure of production, including the mechanical machining process, cleaning and welding process of high vacuum aluminum alloy, ultrahigh vacuum test and so on. In view of the occurrence of the concave deformation, the reasons are analyzed and the correction method developed is introduced too. | Ling Kang (1) ChunHua Li (1) KaiXi Huang (1) HuaMin Qu (1) YiQing Zou (1) Yan Zhang (1) LuXiang Han (1) LingLing Men (1) | 2011 | Science China(Physics,Mechanics & Astronomy)2011，54，S2： | 1 |
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