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| 1 | Interleukin-33 drives hepatic fibrosis through activation of hepatic stellate cells显示文摘Liver fibrosis is a consequence of chronic liver disease,causing morbidity and mortality.Interleukin-33(IL-33)is a critical mediator of inflammation,which may be involved in the development of liver fibrosis.Here,we investigated the role of IL-33 in human patients and experimental bile-duct ligation(BDL)-induced fibrosis in mice.We report increased hepatic IL-33 expression in the murine BDL model of fibrosis and in surgical samples obtained from patients with liver fibrosis.Liver injury,inflammatory cell infiltration and fibrosis were reduced in the absence of the IL-33/ST2 receptor,and the activation of hepatic stellate cells(HSCs)was decreased in ST2-deficient mice.Recombinant IL-33 activated HSCs isolated from C57BL/6 mice,leading to the expression of IL-6,TGF-β,α-SMA and collagen,which was abrogated in the absence of ST2 or by pharmacological inhibition of MAPK signaling.Finally,administration of recombinant IL-33 significantly increased hepatic inflammation in sham-operated BL6 mice but did not enhance BDL-induced hepatic inflammation and fibrosis.In conclusion,BDL-induced liver inflammation and fibrosis are dependent on ST2 signaling in HSCs,and therefore,the IL-33/ST2 pathway may be a potential therapeutic target in human patients with chronic hepatitis and liver fibrosis. | Zhongming Tan Qianghui Liu Runqiu Jiang Long Lv Siamak S Shoto Isabelle Maillet Valerie Quesniaux Junwei Tang Wenjie Zhang Beicheng Sun Bernhard Ryffel | 2018 | Cellular & Molecular Immunology2018，15，4： | 24 |
| 2 | Xiaotan Sanjie decoction attenuates tumor angiogenesis by manipulating Notch-1-regulated proliferation of gastric cancer stem-like cells显示文摘AIM: To determine the underlying mechanisms of action and influence of Xiaotan Sanjie(XTSJ) decoction on gastric cancer stem-like cells(GCSCs). METHODS: The gastric cancer cell line MKN-45 line was selected and sorted by FACS using the cancer stem cell marker CD44; the stemness of these cells was checked in our previous study. In an in vitro study, the expression of Notch-1, Hes1, Vascular endothelial growth factor(VEGF), and Ki-67 in both CD44-positive gastric cancer stem-like cells(GCSCs) and CD44-negative cells was measured by Western blot. The effect of XTSJ serum on cell viability and on the above markers was measured by MTT assay and Western blot, respectively. In an in vivo study, the ability to induce angiogenesis and maintenance of GCSCs in CD44-positive-MKN-45- and CD44-negative-engrafted mice were detected by immunohistochemical staining using markers for CD34 and CD44, respectively. The role of XTSJ decoction in regulating the expression of Notch-1, Hes1, VEGF and Ki-67 was measured by Western blot and real-time polymerase chain reaction.RESULTS: CD44+ GCSCs showed more cell proliferation and VEGF secretion than CD44-negative cells in vitro, which were accompanied by the high expression of Notch-1 and Hes1 and positively associated with tumor growth(GCSCs vs CD44-negative cells, 2.72 ± 0.25 vs 1.46 ± 0.16, P < 0.05) and microvessel density(MVD)(GCSCs vs CD44-negative cells, 8.15 ± 0.42 vs 3.83 ± 0.49, P < 0.001) in vivo. XTSJ decoction inhibited the viability of both cell types in a dose-dependent manner in vitro. Specifically, a significant difference in the medium-(82.87% ± 6.53%) and high-dose XTSJ groups(77.43% ± 7.34%) was detected at 24 h in the CD44+ GCSCs group compared with the saline group(95.42% ± 5.76%) and the low-dose XTSJ group(90.74% ± 6.57%)(P < 0.05). However, the efficacy of XTSJ decoction was reduced in the CD44- groups; significant differences were only detected in the high-dose XTSJ group at 48 h(78.57% ± 6.94%) and 72 h(72.12% ± 7.68%) when compared with the other CD44- groups(P < 0.05). Notably, these differences were highly consistent with the Notch-1, Hes1, VEGF and Ki-67 expression in these cells. Similarly, in vivo, XTSJ decoction inhibited tumor growth in a dose-dependent manner. A significant difference was observed in the medium(1.76 ± 0.15) and high-dose XTSJ(1.33 ± 0.081) groups compared with the GCSCs control group(2.72 ± 0.25) and the low-dose XTSJ group(2.51 ± 0.25)(P < 0.05). We also detected a remarkable decrease of MVD in the medium-(7.10 ± 0.60) and high-dose XTSJ(5.99 ± 0.47) groups compared with the GCSC control group(8.15 ± 0.42) and the low-dose XTSJ group(8.14 ± 0.46)(P < 0.05). Additionally, CD44 expression was decreased in these groups [medium-(4.43 ± 0.45) and high-dose XTSJ groups(3.56 ± 0.31) vs the GCSC control(5.96 ± 0.46) and low dose XTSJ groups(5.91 ± 0.38)](P < 0.05). The significant differences in Notch-1, Hes1, VEGF and Ki-67 expression highly mirrored the results of XTSJ decoction in inhibiting tumor growth, MVD and CD44 expression.CONCLUSION: Notch-1 may play an important role in regulating the proliferation of GCSCs; XTSJ decoction could attenuate tumor angiogenesis, at least partially, by inhibiting Notch-1. | Bing Yan Long Liu Ying Zhao Li-Juan Xiu Da-Zhi Sun Xuan Liu Ye Lu Jun Shi Yin-Cheng Zhang Yong-Jin Li Xiao-Wei Wang Yu-Qi Zhou Shou-Han Feng Can Lv Pin-Kang Wei Zhi-Feng Qin | 2014 | World Journal of Gastroenterology2014，20，36： | 14 |
| 3 | Gold nanorod-photosensitizer conjugate with extracellular pH-driven tumor targeting ability for photothermal/photodynamic therapy显示文摘氯 e6-pHLIP <候补选手class=“ a-plus-plus ”> ss -AuNRs,金使nanorod具有感光性结合包含 pH (低)有给予细胞外的 pH 的一张二硫化物契约的插入肽( pHLIP )( pH <候补选手class=“ a-plus-plus ”> e )-driven 肿瘤指向能力,成功地为光力学的 bimodal 和 photothermal 治疗被开发了。在这 bimodal 治疗,氯 e6 (Ce6 ) ,第二代的 photosensitizer (PS ) ,被用于光力学的治疗(太平洋夏季时间) 。金 nanorods (AuNRs ) 作为 Ce6 的 nanocarrier 和 quencher 为 photothermal 治疗(PTT ) 并且也被用作一个过高热代理人。pHLIPss 作为一根驾驶 pHe 的指向探针被设计在低 pH 在癌症房间提高 Ce6 和 AuNRs 的累积。在 Ce6-pHLIP < 潜水艇 class= “ a-plus-plus ” > ss -AuNRs, Ce6 接近并且由 AuNRs 熄灭了,引起小太平洋夏季时间效果。当暴露了到正常生理的 pH 时 7.4, Ce6-pHLIP < 潜水艇 class= “ a-plus-plus ” > ss -AuNRs 泛泛地与房间膜联系。然而,曾经暴露了到酸的 pH 6.2, pHLIP 活跃地插入到房间膜,和 conjugates 是进房间的 translocated。当这发生时, Ce6 由于二硫化物与 AuNRs 分开细胞内部的谷胱甘肽(GSH ) 引起的契约劈开,和汗衫氧在光之上为太平洋夏季时间被生产照耀。作为单个 PTT 代理人,另外, AuNRs 能由提高的浸透和保留(EPR ) 效果在肿瘤提高 PS 的累积。因此由我们的数据显示了当暴露了到酸的 pH 时, Ce6-pHLIP < 潜水艇 class= “ a-plus-plus ” > ss -AuNRs 能为癌症治疗完成 synergistic PTT/PDT bimodality。 | Nannan Wang Zilong Zhao Yifan Lv Huanhuan Fan Huarong Bai Hongmin Meng Yuqian Long Ting Fu Xiaobing Zhang Weihong Tan | 2014 | Nano Research2014，7，9： | 11 |
| 4 | Advances in Vision-Based Lane Detection:Algorithms,Integration,Assessment,and Perspectives on ACP-Based Parallel Vision显示文摘Lane detection is a fundamental aspect of most current advanced driver assistance systems(ADASs). A large number of existing results focus on the study of vision-based lane detection methods due to the extensive knowledge background and the low-cost of camera devices. In this paper, previous visionbased lane detection studies are reviewed in terms of three aspects, which are lane detection algorithms, integration, and evaluation methods. Next, considering the inevitable limitations that exist in the camera-based lane detection system, the system integration methodologies for constructing more robust detection systems are reviewed and analyzed. The integration methods are further divided into three levels, namely, algorithm, system,and sensor. Algorithm level combines different lane detection algorithms while system level integrates other object detection systems to comprehensively detect lane positions. Sensor level uses multi-modal sensors to build a robust lane recognition system. In view of the complexity of evaluating the detection system, and the lack of common evaluation procedure and uniform metrics in past studies, the existing evaluation methods and metrics are analyzed and classified to propose a better evaluation of the lane detection system. Next, a comparison of representative studies is performed. Finally, a discussion on the limitations of current lane detection systems and the future developing trends toward an Artificial Society, Computational experiment-based parallel lane detection framework is proposed. | Yang Xing Chen Lv Long Chen Huaji Wang Hong Wang Dongpu Cao Efstathios Velenis Fei-Yue Wang | 2018 | IEEE/CAA Journal of Automatica Sinica2018，5，3： | 11 |
| 5 | The Coix Genome Provides Insights into Panicoideae Evolution and Papery Hull Domestication显示文摘Coix is a grass crop domesticated as early as the Neolithic era.It is still widely cultivated for both highly nutritional food and medicinal use.However,the genetic study and breeding of this crop are hindered by the lack of a sequenced genome.Here,we report de novo sequencing and assembly of the 1619-Mb genome of Coix,and annotation of 75.39%repeats and 39629 protein-coding genes.Comparative genomics analysis showed that Coix is more closely related to sorghum than maize,but intriguingly only Coix and maize had a recent genome duplication event,which was not detected in sorghum.We further constructed a genetic map and mapped several important traits,especially the strength of hull.Selection of papery hull(thin:easy dehulling)from the stony hull(thick:difficult dehulling)in wild progenitors was a key step in Coix domestication.The papery hull makes seed easier to process and germinate.Anatomic and global transcriptome analysis revealed that the papery hull is a result of inhibition of cell division and wall biogenesis.We also successfully demonstrated that seed hull pressure resistance is controlled by two major quantitative trait loci(QTLs),which are associated with hull thickness and color,respectively.The two QTLs were further fine mapped within intervals of 250 kb and 146 kb,respectively.These resources provide a platform for evolutionary studies and will facilitate molecular breeding of this important crop. | Chao Guo Yanan Wang Aiguo Yang Jun He Chaowen Xiao Shanhua Lv Fengming Han Yibing Yuan Yuan Yuan Xiaolong Dong Juan Guo YawenYang-Hailan Liu Ningzhi Zuo Yaxi Hu Kangxu Zhao Zhengbo Jiang Xing Wang Tingting Jiang Yaou Sherf Moju Cao Yuan Wang Zhaobo Long Tingzhao Rong Luqi Huang Shufeng Zhou | 2020 | Molecular Plant2020，13，2： | 11 |
| 6 | The effect of fire disturbance on short-term soil respiration in typical forest of Greater Xing'an Range, China显示文摘We investigated the effect of fire disturbance on short-term soil respiration in birch(Betula platyphylla Suk.) and larch(Larix gmelinii Rupr.) forests in Greater Xing'an range, northeastern China for further understanding of its effect on the carbon cycle in ecosystems. Our study show that post-fire soil respiration rates in B. platyphylla and L. gmelinii forests were reduced by 14% and 10%, respectively. In contrast, the soil heterotrophic respiration rates in the two types of forest were similar in post-fire and control plots. After fire, the contribution of root respiration to total soil respiration was dramatically reduced. Variation in soil respiration rates was explained by soil moisture(W) and soil temperature(T) at a depth of 5 cm. Exponential regression fitted T and W models explained Rs rates in B. platyphylla control and post-fire plots(83.1% and 86.2%) and L. gmelinii control and post-fire plots(83.7% and 88.7%). In addition, the short-term temperature coefficients in B.platyphylla control and post-fire plots were 5.33 and 5, respectively, and 9.12, and 5.26 in L. gmelinii control and post-fire plots, respectively. Our results provide an empirical baseline for studying the effect of fire disturbance on soil carbon balance and estimation of soil carbon flux in boreal forest. | Long Sun Tongxin Hu Ji Hong Kim Futao Guo Hong Song Xinshuang Lv Haiqing Hu | 2014 | Journal of Forestry Research2014，25，3： | 11 |
| 7 | Mechanical Properties and Microstructure of 6082-T6 Aluminum Alloy Joints by Self-support Friction Stir Welding显示文摘The majority of this research has concentrated on developing the self-support friction stir welding(SSFSW) tool which consists of a big concave upper shoulder and a small convex lower shoulder, and procedures for making reliable welds in aluminum hollow extrusion. The 5-mm-thick 6082-T6 aluminum alloy was self-support friction stir welded at a constant tool rotation speed of 800 r/min. The effect of welding speed on microstructure and mechanical properties was investigated. The results of transverse tensile test indicated that the tensile strength of joints increased and the elongation decreased with increasing welding speed. The whole values of microhardness of SSFSW joints increased with increasing welding speed from 10 to 200 mm/min. The defectfree joints were obtained at lower welding speeds and the tensile fracture was located at the heat-affected zone(HAZ) adjacent to the thermo-mechanically affected zone(TMAZ) on the advancing side. The investigation of the flow pattern of the softened metal around the SSFSW tool revealed that the flow pattern of the softened metal was driven by two shoulders and the stir pin. The failure of specimens in tension presented the ductile fracture mode. | Long Wan Yongxian Huang Weiqiang Guo Shixiong Lv Jicai Feng | 2014 | Journal of Materials Science & Technology2014，30，12： | 10 |
| 8 | Biodiversity-based development and evolution:the emerging research systems in model and non-model organisms显示文摘Evolutionary developmental biology,or Evo-Devo for short,has become an established field that,broadly speaking,seeks to understand how changes in development drive major transitions and innovation in organismal evolution.It does so via integrating the principles and methods of many subdisciplines of biology.Although we have gained unprecedented knowledge from the studies on model organisms in the past decades,many fundamental and crucially essential processes remain a mystery.Considering the tremendous biodiversity of our planet,the current model organisms seem insufficient for us to understand the evolutionary and physiological processes of life and its adaptation to exterior environments.The currently increasing genomic data and the recently available gene-editing tools make it possible to extend our studies to non-model organisms.In this review,we review the recent work on the regulatory signaling of developmental and regeneration processes,environmental adaptation,and evolutionary mechanisms using both the existing model animals such as zebrafish and Drosophila,and the emerging nonstandard model organisms including amphioxus,ascidian,ciliates,single-celled phytoplankton,and marine nematode.In addition,the challenging questions and new directions in these systems are outlined as well. | Long Zhao Feng Gao Shan Gao Yujun Liang Hongan Long Zhiyi Lv Ying Su Naihao Ye Liusuo Zhang Chengtian Zhao Xiaoyu Wang Weibo Song Shicui Zhang Bo Dong | 2021 | Science China(Life Sciences)2021，64，8： | 9 |
| 9 | Association between NOD2/CARD15 gene polymorphisms and Crohn's disease in Chinese Zhuang patients显示文摘AIM:To assess the relationship between the P268S,JW1 and N852S polymorphisms and Crohn’s disease(CD)susceptibility in Zhuang patients in Guangxi,China.METHODS:Intestinal tissues from 102 Zhuang[48CD and 54 ulcerative colitis(UC)]and 100 Han(50 CD and 50 UC)unrelated patients with inflammatory bowel disease and 72 Zhuang and 78 Han unrelated healthy individuals were collected in the Guangxi Zhuang Autonomous Region from January 2009 to March 2013.Genomic DNA was extracted using the phenol chloroform method.The P268S,JW1 and N852S polymorphisms were amplified using polymerase chain reaction(PCR),detected by restriction fragment length polymorphism(RFLP),and verified by gene sequencing.RESULTS:Heterozygous mutation of P268S in the NOD2/CARD15 gene was detected in 10 CD cases(six Zhuang and four Han),two Han UC cases,and one Zhuang healthy control,and P268S was strongly associated with the Chinese Zhuang and Han CD populations(P=0.016 and 0.022,respectively).No homozygous mutant P268S was detected in any of the groups.No significant difference was found in P268S genotype and allele frequencies between UC and control groups(P>0.05).Patients with CD who carried P268S were likely to be≤40 years of age(P=0.040),but were not significantly different with regard to race,lesion site,complications,and other clinical features(P>0.05).Neither JW1 nor N852S polymorphisms of the NOD2/CARD15gene were found in any of the subjects(P>0.05).CONCLUSION:P268S polymorphism may be associated with CD susceptibility in the Zhuang population in the Guangxi Zhuang Autonomous Region,China.In contrast,JW1 and N852S polymorphisms may not be related to CD susceptibility in these patients. | Wei-Yan Long Lan Chen Cui-Liang Zhang Rong-Mao Nong Mei-Jiao Lin Ling-Ling Zhan Xiao-Ping Lv | 2014 | World Journal of Gastroenterology2014，20，16： | 7 |
| 10 | Modulation characteristics and microwave generation for AlGaInAs/InP microring lasers under four-wave mixing显示文摘The dynamic characteristics are investigated for a microring laser with an external radius of 12μm subject to external optical injection.Single-mode operation with a side mode suppression ratio of 33.4 dB is realized at a biasing current of 25 mA and a temperature of 290 K,and the corresponding small-signal modulation response is obtained with a resonance peak frequency of 7.5 GHz.Under the optical injection from a tunable laser,the improvements of the small-signal modulation response induced by four-wave mixing are observed for the microring laser,which shows an additional resonance peak around the frequency of the beat frequency between the lasing mode and the injecting light.Furthermore,optical generation of a microwave signal is realized by the light beating between the lasing mode and the injecting light measured by a high-speed photodetector and a spectrum analyzer. | Ling-Xiu Zou Yong-Zhen Huang Xiao-Meng Lv Bo-Wen Liu Heng Long Yue-De Yang Jin-Long Xiao Yun Du | 2014 | Photonics Research2014，2，6： | 6 |
| 11 | Photocatalytic degradation of amoxicillin by carbon quantum dots modified K2Ti6O13 nanotubes:Effect of light wavelength显示文摘A novel carbon quantum dots modified potassium titanate nanotubes(CQDs/K2 Ti6 O13)composite photocatalyst was synthesized by hydrothermal treatment combined with calcination.X-ray diffraction(XRD)pattern and transmission electron microscopy(TEM)indicated formation of potassium titanate nanotubes and successful deposition of CQDs onto K2 Ti6 O13.The photocatalytic performance of CQDs/K2 Ti6 O13 composite was evaluated by degradation of amoxicillin(AMX)under the irradiation of visible light and lights with the wavelengths of 365,385,420,450,485,520,595 and 630 nm.The results showed that the photocatalytic activity of CQDs/K2 Ti6 O13 hybrid material was greatly enhanced compared with the neat K2 Ti6 O13 calcined at 300 ℃.The narrowed band gap energy(Eg)and transfer of photo-excited electron by CQDs inhibited the immediate combination of electron-hole pairs,thus promoting photocatalytic activity.Moreover,CQ,Ds/K2 Ti6 O13 exhibited a broad spectrum of photocatalytic ability and it was interesting that the photocatalytic activity decreased with the increase of the irradiation wavelength.Reactive oxygen species(ROS)quenching tests suggested the hole(h^+)and hydroxyl radical(^·OH)played the primary roles in photocatalytic degradation of AMX.Moreover,CQ.Ds/K2 Ti6 O13 showed good reusability for AMX photocatalytic degradation after five successive runs.This study proposed an available method for titanate nanomaterials modification,and the developed novel CQDs/K2 Ti6 O13hyb rid material is p ro mising fo r potential application on antibiotics removal fro m water and wastewater. | Qiankun Chen Long Chen Juanjuan Qi Yingqian Tong Yitao Lv Chaokai Xu Jinren Ni Wen Liu | 2019 | Chinese Chemical Letters2019，30，6： | 6 |
| 12 | Polymorphism-Dependent Dynamic Ultralong Organic Phosphorescence显示文摘Developing ultralong organic phosphorescence(UOP)materials with smart response to external stimuli is of great interest in photonics applications,whereas the manipulation of molecular stacking on tuning such dynamic UOP is still a formidable challenge.Herein,we have reported two polymorphs with distinct photoactivated dynamic UOP behavior based on a pyridine derivative for the first time.Our experiment revealed that the dynamic UOP behavior including photoactivation and deactivation feature is highly dependent on irradiation intensity and environmental atmosphere.Additionally,given the unique dynamic UOP feature,these phosphors have been successfully applied to phosphorescence-dependent molecular logic gate and timing data storage.This result not only paves a way to design smart functional materials but also expands the scope of the applications on organic phosphorescence materials. | Mingxing Gu Huifang Shi Kun Ling Anqi Lv Kaiwei Huang Manjeet Singh He Wang Long Gu Wei Yao Zhongfu An Huili Ma Wei Huang | 2020 | Research2020，，1： | 5 |
| 13 | Active/passive Q-switching operation of 2 μm Tm,Ho:YAP laser with an acousto-optical Q-switch/MoS_2 saturable absorber mirror显示文摘The active/passive Q-switching operation of a 2 μm a-cut Tm,Ho:YAP laser was experimentally demonstrated with an acousto-optical Q–switch∕MoS_2 saturable absorber mirror. The active Q-switch laser was operated for the first time, to the best of our knowledge, with an average output power of 12.3 W and a maximum pulse energy of 10.3 mJ. The passive Q-switch laser was also the first acquired with an average output power of 3.3 W and per pulse energy of 23.31 μJ, and the beam quality factors of M_x^2 = 1.06 and M_y^2 = 1.06 were measured at the average output power of 2 W. | LINJUN LI XINING YANG LONG ZHOU WENQIANG XIE YUNLONG WANG YINGJIE SHEN YUQIANG YANG WENLONG YANG WEI WANG ZHIWEI LV XIAOMING DUAN MINGHUA CHEN | 2018 | Photonics Research2018，6，6： | 5 |
| 14 | Functional distinction of rat liver natural killer cells from spleen natural killer cells under normal and acidic conditions in vitro显示文摘BACKGROUND: The microenvironment within solid tumors has often been shown to exhibit an acidic extracellular pH. Although the morphologic and functional differences in natural killer (NK) cells of the liver and spleen have been reported previously under physiological conditions, the difference under acidic conditions is still unclear. This study was to investigate the differences in the morphological and functional characteristics between rat liver and spleen NK cells under normal and acidic conditions in vitro. METHODS: Liver and spleen NK cells were isolated and purified from Sprague-Dawley rats by density gradient centrifugation and the Dynabeads FlowComp TM Flexi system, and stimulated for 4 days with or without IL-2 or treated with low pH or control for different times. Morphology was examined by scanning electron microscopy (SEM) and transmission electron microscopy (TEM), cell death and proliferation assays were performed by flow cytometry, IFN-γ production was tested by ELISA, and cytotoxic activity was evaluated by lactate dehydrogenase (LDH) release assay. RESULTS: Liver NK cells had significantly higher levels of cytotoxic activity than spleen NK cells under normal and acidic conditions, and the maximum difference was observed at pH 5.6. Further analysis revealed that the cytotoxic activity of NK cells was correlated with morphology, cell death, proliferative activity and IFN-γ production. By TEM, liver NK cells contained a greater number of electron-dense granules per cell at pH 5.6.Moreover, a modest elevation of cell death and reduction of proliferation of liver NK cells occurred within a range of 5.6-7.2. Interestingly, an acidic extracellular pH only marginally, and not significantly, suppressed IFN-γ production by liver NK cells. CONCLUSION: The sharp morphological and functional differences shown by the two types of NK cells in vitro indicate that liver NK cells are unexpectedly resistant to pH shock. | Li-Hong Lv, Jian-Dong Yu, Guo-Lin Li, Tian-Zhu Long, Wei Zhang, Ya-Jin Chen, Jun Min and Yun-Le Wan Department of Hepatobiliary Surgery, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University Department of Breast Surgery, Guangzhou Women and Children’s Medical Center Guangzhou 510120, China Department of Hepatobiliary and Pancreatic Surgery, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China | 2012 | Hepatobiliary & Pancreatic Diseases International2012，11，3： | 5 |
| 15 | The RNA-binding protein Musashi2 governs osteoblast-adipocyte lineage commitment by suppressing PPARγsignaling显示文摘Osteoporosis caused by aging is characterized by reduced bone mass and accumulated adipocytes in the bone marrow cavity. How the balance between osteoblastogenesis and adipogenesis from bone marrow mesenchymal stem cells(BMSCs) is lost upon aging is still unclear. Here, we found that the RNA-binding protein Musashi2(Msi2) regulates BMSC lineage commitment. Msi2 is commonly enriched in stem cells and tumor cells. We found that its expression was downregulated during adipogenic differentiation and upregulated during osteogenic differentiation of BMSCs. Msi2 knockout mice exhibited decreased bone mass with substantial accumulation of marrow adipocytes, similar to aging-induced osteoporosis. Depletion of Msi2 in BMSCs led to increased adipocyte commitment. Transcriptional profiling analysis revealed that Msi2 deficiency led to increased PPARγ signaling.RNA-interacting protein immunoprecipitation assays demonstrated that Msi2 could inhibit the translation of the key adipogenic factor Cebpα, thereby inhibiting PPAR signaling. Furthermore, the expression of Msi2 decreased significantly during the aging process of mice, indicating that decreased Msi2 function during aging contributes to abnormal accumulation of adipocytes in bone marrow and osteoporosis. Thus, our results provide a putative biochemical mechanism for aging-related osteoporosis, suggesting that modulating Msi2 function may benefit the treatment of bone aging. | Jinlong Suo Sihai Zou Jinghui Wang Yujiao Han Lingli Zhang Chenchen Lv Bo Jiang Qian Ren Long Chen Lele Yang Ping Ji Xianyou Zheng Ping Hu Weiguo Zou | 2022 | Bone Research2022，10，3： | 5 |
| 16 | Healing of Stoma After Magnetic Biliary-Enteric Anastomosis in Canine Peritonitis Models显示文摘Objective To assese the healing of stoma after magnetic anastomosis for the reconstruction of biliary-enteric continuity under severe inflammation. Methods Acute bile duct injury was constructed as a bile peritonitis model in mongrel dogs(n=32). Magnetic anastomosis(group A, n=16) and traditional suture anastomosis(group B, n=16) were performed to reconstruct the biliary-enteric continuity in one stage. Half of the dogs in each group were euthanized on the 30 th postoperative day, and the other half on the 90 th postoperative day to harvest the stoma region. The healing conditions of the stoma after the 2 anastomotic approaches were observed with naked eyes, under light microscope and scanning electron microscope. Results The stoma leakage rate(50% versus 0% on the 30 th postoperative day, 37.5% versus 12.5% on the 90 th postoperative day, both P<0.05) and stenosis degree(13.9%±0.3% versus 7.1%±0.3% on the 30 th postoperative day, 17.2%±0.4% versus 9.4%±0.4% on the 90 th postoperative day, both P<0.01) were significantly higher in group B than in group A. Compared with traditional manual anastomoses, the histological analysis under light and electron microscope showed a more continuous stoma with more regular epithelium proliferation and collagen arrangement, less inflammation in group A. Conclusions Magnetic anastomosis stent ensures better healing of the stoma even under the circumstance of severe inflammation. | Jian-hui Li Long Guo Wei-jie Yao Zhi-yong Zhang Shan-pei Wang Shi-qi Liu Zhi-min Geng Xiao-ping Song Yi Lv | 2014 | Chinese Medical Sciences Journal2014，29，2： | 4 |
| 17 | Removal of perchlorate from aqueous solution by cross-linked Fe(Ⅲ)-chitosan complex显示文摘Cross-linked Fe(III)-chitosan composite(Fe-CB) was used as the adsorbent for removing perchlorate from the aqueous solution. The adsorption experiments were carried out by varying contact time, initial concentrations, temperatures, pH, and the presence of co-existing anions. The morphology of the adsorbent was discussed using FT-IR and SEM with X-EDS analysis. The pH ranging from 3.0–10.2 exhibited very little effect on the adsorption capability. The perchlorate uptake onto Fe-CB obeyed Langmuir isotherm model. The adsorption process was rapid and the kinetics data obeyed the pseudo second-order model well. The eluent of 2.5%(W/V) NaCl could regenerate the exhausted adsorbent efficiently. The adsorption mechanism was also discussed. | Long Lv Yanhua Xie Guoming Liu Guo Liu Jing Yu | 2014 | Journal of Environmental Sciences2014，26，4： | 4 |
| 18 | An improved substrate cocktail for assessing direct inhibition and time-dependent inhibition of multiple cytochrome P450s显示文摘瞄准:底层鸡尾酒经常被用来评估细胞色素 P450 (CYP ) 在探查底层之中的调停酶的药相互作用和潜在的相互作用。这里,我们重新优化增加为候选人屏蔽的可靠性和精确性的底层鸡尾酒方法加重并且从直接 CYP 抑制试金扩展了方法到时间依赖者抑制(TDI ) assay.Methods:在包含人的肝微体(0.1 mg/mL ) 的反应混合物,两个都,底层鸡尾酒(为 1A2,为 2A6 的香豆素,为 2B6 的 bupropion,为 2C9 的 diclofenac,为 2D6 的 dextromethorphan,和为 3A4 的睾丸激素的非那西汀) 和孵化时间的集中被优化。底层探针的代谢物被(MRM ) 使用平淡的 LC/MS/MS 的多重反应的监视同时分析。直接 CYP 抑制用 7 个禁止者被验证(α -naphthoflavone, tranylcypromine, ticlopidine, fluconazole,奎尼定, ketoconazole 和 1-ABT ) 。时间依赖者抑制部分与 5 个禁止者(ketoconazole, verapamil,奎尼定, paroxetine 和 1-ABT ) 被验证 .Results:当单个底层和底层鸡尾酒被测试时,抑制曲线侧面和 7 个 CYP 禁止者的 IC 50 价值是近似的,并且与以前报导的价值一致。当单个底层和底层鸡尾酒在 TDI assay.Conclusion 被测试时,类似的结果在 5 个禁止者的 IC 50 移动被获得:6-in-1 底层鸡尾酒(为 1A2, 2A6, 2B6, 2C9, 2D6 和 3A ) 为估计 CYP 抑制和药候选人的时间依赖者抑制是可靠的。 | Zhong-hua CHEN Su-xing ZHANG Na LONG Li-shan LIN Tao CHEN Fei-peng ZHANG Xue-qin LV Pei-zhen YE Ning LI Ke-zhi ZHANG | 2016 | Acta Pharmacologica Sinica2016，37，5： | 4 |
| 19 | Genomic characterizations of H4 subtype avian influenza viruses from live poultry markets in Sichuan province of China, 2014–2015显示文摘Dear Editor,Avian influenza viruses (AIVs) have posed a serious threat to poultry production and public health. To date, more than fourteen AIV subtypes that are able to infect human beings have been documented. Also, it is suggested that new subtypes may be reported in the future, owing to the migration of wild birds and live poultry transportation (Gao, 2018).Poultry may act as a potential incubator for novel subtypes of avian influenza virus (Bi et al., 2016a; Bi et al., 2016b; Liu et al., 2014a; Su et al., 2017). Up to date, the H7N9 AIV emerged in February 2013 has caused 1,567 human cases,with a fatality rate of 39.2%(http://gffzz36b398b81f8a4a4ch0b66bp0cqcfw69qb.ffgz.tsg.suse.edu.cn/influenza/ | Chuansong Quan Ting Huang Xiuwei Chen Jie Zhang Qianli Wang Cheng Zhang Tiyan Zhang Lijun Zhou Liumei Shu Chunrong Long Lei Yang Xunbo Du Yingze Zhao Peipei Liu Hao Song Weifeng Shi Yuhai Bi Qiang Lv William J.Liu George F.Gao | 2018 | Science China(Life Sciences)2018，61，9： | 4 |
| 20 | Color-tunable ultralong organic phosphorescence materials for visual UV-light detection显示文摘Ultralong organic phosphorescence(UOP)materials have roused considerable attention in the field of photonics and optoelectronics owing to the feature of long-lived emission lifetimes.However,to develop UOP materials with color-tunability is still a formidable challenge.Here,we report a class of UOP materials containing carbonyl,amino or amide groups,exhibiting colortunable persistent luminescence ranging from blue(458 nm)to yellow-green(508 nm)under different UV wavelength excitation.Taken theoretical and experimental results together,we conclude that the excitation dependent color-tunable UOP emission is ascribed to multiple emission centers from single molecular and aggregated states in crystal.Given color-tunable UOP feature,these materials are used to successfully realize visual UV-light detection.This finding not only provides a strategy to design new organic phosphorescent molecules with colorful emission,but also extends the scope of the applications of purely organic phosphorescent materials. | Lifang Bian Huili Ma Wenpeng Ye Anqi Lv He Wang Wenyong Jia Long Gu Huifang Shi Zhongfu An Wei Huang | 2020 | Science China Chemistry2020，63，10： | 3 |
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