|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Qingnaoyizhi decoction suppresses the formation of glial fibrillary acidic protein-positive cells in cultured neural stem cells by inhibiting the Janus kinase 2/signal transducer and activator of transcription 3 signaling pathway显示文摘OBJECTIVE: Inactivation of the Janus kinase 2(JAK2)/signal transducer and activator of transcription 3(STAT3) signaling axis plays a crucial role in determining the fate of neural stem cells(NSCs).Qingnaoyizhi decoction(QNYZD) has been used for the treatment of vascular dementia and has shown to improve synaptic remodeling. The aim of this study was to evaluate the effect of cerebrospinal fluid(CSF) containing QNYZD(CSF-QNYZD) on the differentiation of cultured NSCs and the involvement of the JAK2/STAT3 pathway.METHODS: The protein expression levels of glial fibrillary acidic protein(GFAP), tubulin, drosophila mothers against decapentaplegic protein(SMAD-1), STAT3, and phosphorylated-STAT3 were detected by western immunoblot analysis in the groups: control, CSF, JAK/STAT inhibitor(AG490),CSF-QNYZD, and CSF-XDZ(CSF-Xidezhen). The differentiation of NSCs was determined by immunofluorescence staining. The proliferation of NSCs was measured using the Cell Counting Kit-8 proliferation assay.RESULTS: Compared with the control group,CSF-QNYZD and AG490 significantly increased the number and expression of tubulin-positive cells, reduced the number and expression of GFAP-positive cells, and down-regulated the expression of p-STAT3. However, CSF-QNYZD also decreased the expression of SMAD-1 and STAT3.CONCLUSION: Enhanced neuronal differentiation may be associated with the down-regulation of glial differentiation instead of promoting proliferationin treated NSCs. Furthermore, QNYZD may play a direct role in suppressing the formation of GFAP-positive cells and enhancing neuronal differentiation by inhibiting JAK2/STAT3 activation. Overall, these results provide insights into the possible mechanism underlying QNYZD-mediated neurogenesis. | Wu Yanqing Jing Zhiwei Qin Xiude Zhou Zhen Wang Kai Song Wanshan Wang Xueyan Hou Mengmeng Zhang Yulian Kang Liyuan | 2015 | Journal of Traditional Chinese Medicine2015，35，1： | 11 |
| 2 | A new unconventional HLA-A2-restricted epitope from HBV core protein elicits antiviral cytotoxic T lymphocytes显示文摘细胞毒素的 T 房间(CTL ) 在肝炎 B 的控制起一个关键作用病毒(HBV ) 感染和病毒的清理。然而,大多数识别 CTL epitopes 从遗传型 A 和 D 的 HBV 被导出,并且很少在在亚洲更流行的遗传型 B 和 C 的病毒被定义。因为 HBV 核心蛋白质(HBc ) 是最保守、产生免疫性的部件,在这研究,我们使用了盖住 HBc 屏蔽并且识别特定的 CTL epitopes 的一个重叠 9-mer 肽水池。异乎寻常的 HLA-A2-restricted epitope HBc141149 被结晶化分析发现,在结构上描绘了。immunogenicity 和 anti-HBV 活动进一步在 HBV 和 HLA-A2 被决定转基因的老鼠。最后,我们证明在 HBc141149 epitope 的变化与病毒的参数被联系,在 HBV 的疾病前进感染了病人。我们的数据因此提供卓见进这异乎寻常的 epitope 绑定的结构特征给 MHC-I 分子,以及 epitope 特定的 CTL 活动在 HBV 安排 T 房间反应和有免疫力的避免感染的病人。 | Lu Sun Yu Zhang Bao Zhao Mengmeng Deng Jun Liu Xin Li Junwei Hou Mingming Gui Shuijun Zhang Xiaodong Li George F. Gao Songdong Meng | 2014 | Protein & Cell2014，5，4： | 7 |
| 3 | New research trends on high-precision time transfer technology显示文摘High-precision time transfer plays an important role in the areas of fundamental research and applications. Accompanying w ith the remarkable improvements in the ability of generating and measuring high-accuracy time-frequency signal,seeking for new time-transfer techniques betw een distant clocks w ith much further improved accuracy attracts attentions w orld-w idely. The time-transfer technique based on optical pulses has the highest precision presently,and the further improvement in the accuracy is heavily dependent on the time-domain properties of the pulse as w ell as the sensitivity of the applied measurement on the exchanged pulse. The application of optical frequency comb in time transfer for a precision up to femtosecond level are currently the focus of much interest,and has recently achieved many breakthroughs. Further investigations show that,utilizing quantum techniques,i.e. quantum measurement technique and quantum optical pulse source,can lead to a new limit on the measured timing information. Furthermore,it can be immune from atmospheric parameters,such as pressure,temperature,humidity and so on.Such quantum improvements on time-transfer have a bright prospect in the future applications requiring extremely high-accuracy timing and ranging. The potential achievements w ill form a technical basis for the future realization of sub-femtosecond time transfer system. | DONG Ruifang QUAN Run'ai HOU Feiyan WANG Shaofeng XIANG Xiao ZHOU Conghua WANG Mengmeng LIU Tao ZHANG Shou'gang | 2015 | Instrumentation2015，2，4： | 5 |
| 4 | Metformin activates chaperone-mediated autophagy and improves disease pathologies in an Alzheimer disease mouse model显示文摘Chaperone-mediated autophagy(CMA)is a lysosome-dependent selective degradation pathway implicated in the pathogenesis of cancer and neurodegenerative diseases.However,the mechanisms that regulate CMA are not fully understood.Here,using unbiased drug screening approaches,we discover Metformin,a drug that is commonly the first medication prescribed for type 2 diabetes,can induce CMA.We delineate the mechanism of CMA induction by Metformin to be via activation of TAK1-IKKα/β signaling that leads to phosphorylation of Ser85 of the key mediator of CMA,Hsc70,and its activation.Notably,we find that amyloid-beta precursor protein(APP)is a CMA substrate and that it binds to Hsc70 in an IKKα/β-dependent manner.The inhibition of CMA-mediated degradation of APP enhances its cytotoxicity.Importantly,we find that in the APP/PS1 mouse model of Alzheimer's disease(AD),activation of CMA by Hsc70 overexpression or Metformin potently reduces the accumulated brain Aβplaque levels and reverses the molecular and behavioral AD phenotypes.Our study elucidates a novel mechanism of CMA regulation via Metformin-TAK1-IKKα/β-Hsc70 signaling and suggests Metformin as a new activator of CMA for diseases,such as AD,where such therapeutic intervention could be beneficial. | Xiaoyan Xu Yaqin Sun Xufeng Cen Bing Shan Qingwei Zhao Tingxue Xie Zhe Wang Tingjun Hou Yu Xue Mengmeng Zhang Di Peng Qiming Sun Cong Yi Ayaz Najafov Hongguang Xia | 2021 | Protein & Cell2021，12，10： | 5 |
| 5 | Polydopamine(PDA)-activated cobalt sulfide nanospheres responsive to tumor microenvironment(TME)for chemotherapeutic-enhanced photothermal therapy显示文摘Most recently,cobalt sulfide(CoS)nanospheres(NSs)have been demonstrated as an ideal high-efficient photothermal agent for tumor elimination.Howeve r,the surface of CoS NSs is lack of functio nal chemical groups or active radicals to incorporate therapeutic agents,which tremendously hinders their versatile utilization in medical field.Here,surface activation of CoS NSs was realized through the growth of polydopamine(PDA)in situ via alkaline-triggered polymerization.Upon the formation of CoS@PDA NSs,thiol-polyethylene glycol(SH-PEG)and chemotherapeutic agent of doxorubicin(DOX)were loaded onto the particle surface by means ofπ-πelectrostatic interaction and Michael addition reactions.Assynthesized CoS@PDA/PEG/DOX(CoPPD)NSs exhibited an admirable photothermal property and high loading capacity of DOX(44.6%).Furthermore,drug release can be accelerated under a more acidic pH condition mimicking tumor microenvironment(TME),ascribed to the protonation of amino group in DOX molecules.Finally,a strong chemotherapeutic-enhanced photothermal therapeutic effect was demonstrated toward solid tumor under near-infrared(NIR)light irradiation without causing significant systemic toxicity.In this regard,this paradigm may offer valuable guidance for the design of multifunctional CoS-based nanoagents for medical treatment. | Mengmeng Hou Yuanxin Zhong Lei Zhang Zhigang Xu Yuejun Kang Peng Xue | 2021 | Chinese Chemical Letters2021，32，3： | 2 |
| 6 | Adverse Effect of Nonalcoholic Fatty Liver Disease on the Therapeutic Response in Patients with Chronic Hepatitis B显示文摘Background and Aims:The impact of nonalcoholic fatty liver disease(NAFLD)on the treatment outcome of chronic hepatitis B(CHB)is undefined and deserves an in-depth investigation.Methods:Histologically-proven CHB receiving first-line antiviral regimens as initial therapy was enrolled and grouped by the concurrence of NAFLD,and followed up at six monthly intervals.Therapeutic response related data were recorded and compared at multiple time points.Kaplan-Meier and Cox regression analyses were utilized to estimate the impact of NAFLD on complete virological response(CVR).Results:We enrolled 267 patients(CHB:164;CHB with NAFLD:103)with comparable follow-up durations.They were also comparable in baseline HBV DNA levels and HBeAg positivity.Patients with concomitant NAFLD showed less significant decline in HBV DNA,qHBsAg,pgRNA,and liver enzyme levels over time;moreover,their cumulative incidences of CVR were significantly lower and that of low-level viremia(LLV)were significantly higher at 6,12,18,24 months.First CVR of CHB was delayed with the presence NAFLD(11.0 vs.7.0 months,p<0.001)and further prolonged with higher grade of liver steatosis(Grade 2–3 vs.1:13.0 vs.9.0 months).On multivariate analysis,HBeAg positivity(HR:0.650,p=0.036),grade of steatosis(G2[HR:0.447,p=0.004];G3[HR:0.085,p=0.002])and HBV DNA(log10 IU/mL)(HR:0.687,p<0.001)were significantly associated with delayed CVR,whereas grade of necroinflammation(HR:1.758,p<0.001)accelerated the CVR.Conclusions:In CHB patients receiving initial antiviral therapy,NAFLD was associated with higher levels of HBV DNA,pgRNA,and liver enzymes,and higher incidence of LLV and delayed CVR. | Siyu Zhang Xiaoxiao Zhang Huiming Jin Yao Dou Lu Li Xiwei Yuan Chen Dong Mengmeng Hou Yue-min Nan Jia Shang | 2023 | Journal of Clinical and Translational Hepatology2023，11，1： | 1 |
| 7 | Corneal subbasal nerve fiber regeneration in myopic patients after laser in situ keratomileusis显示文摘A total of 26 myopic patients(52 eyes) underwent laser in situ keratomileusis.In vivo confocal microscopy revealed that most of the regenerated corneal subbasal nerve fibers in the corneal flap originated from the stump of corneal subbasal nerve fibers outside the ablation zone and extended towards the center of the cornea in all patients.Meanwhile,new fibers were also found to directly regenerate from deep in the stroma in some cases.Approximately 94% of regenerated corneal subbasal nerve fibers(73/78 eyes) regrew vertically into the peripheral central 6-mm circle area 1 month after surgery,78%(28/36 eyes) grew into the central 3-6 mm area at 2 months,and 23% into the central 3-mm circle area at 3 months.In addition,there was no significant difference in corneal subbasal nerve fiber regenerative capacity between the basic fibroblast growth factor group and the 20%(v/v) deproteinized extract of calf blood group.The majority of corneal subbasal nerve fiber regeneration occurred from the stump of corneal subbasal nerve fibers outside the corneal flap,and the remaining growth occurred deep within the stroma. | Shijing Deng Mengmeng Wang Fengju Zhang Xuguang Sun Wenbo Hou Ning Guo | 2012 | Neural Regeneration Research2012，7，20： | 1 |
| 8 | Sustainability of SARS-CoV-2 Induced Humoral Immune Responses in COVID-19 Patients from Hospitalization to Convalescence Over Six Months显示文摘Understanding the persistence of antibody in convalescent COVID-19 patients may help to answer the current major concerns such as the risk of reinfection,the protection period of vaccination and the possibility of building an active herd immunity.This retrospective cohort study included 172 COVID-19 patients who were hospitalized in Wuhan.A total of404 serum samples were obtained over six months from hospitalization to convalescence.Antibodies in the specimens were quantitatively analyzed by the capture chemiluminescence immunoassays(CLIA).All patients were positive for the anti-SARS-Co V-2 Ig M/Ig G at the onset of COVID-19 symptoms,and the Ig G antibody persisted in all the patients during the convalescence.However,only approximately 25%of patients can detect the Ig M antibodies,Ig M against N protein(NIg M)and receptor binding domain of S protein(RBD-Ig M)at the 27 th week.The titers of Ig M,N-Ig M and RBD-Ig M reduced to 16.7%,17.6%and 15.2%of their peak values respectively.In contrast,the titers of Ig G,N-Ig G and RBD-Ig G peaked at 4–5 th week and reduced to 85.9%,62.6%and 87.2%of their peak values respectively at the end of observation.Dynamic behavior of antibodies and their correlation in age,gender and severity groups were investigated.In general,the COVID-19 antibody was sustained at high levels for over six months in most of the convalescent patients.Only a few patients with antibody reducing to an undetectable level which needs further attention.The humoral immune response against SARS-Co V-2 infection in COVID-19 patients exhibits a typical dynamic of acquired immunity. | Yang Zheng Qing Zhang Ashaq Ali Ke Li Nan Shao Xiaoli Zhou Zhiqin Ye Xiaomin Chen Shanshan Cao Jing Cui Juan Zhou Dianbing Wang Baidong Hou Min Li Mengmeng Cui Lihua Deng Xinyi Sun Qian Zhang Qinfang Yang Yong li Hui Wang Yake Lei Bo Yu Yegang Cheng Xiaolin Tong Dong Men Xian-En Zhang | 2021 | Virologica Sinica2021，36，5： | 1 |
| 9 | Incidence,genomic diversity,and evolution of strawberry mottle virus in China显示文摘Strawberry mottle virus(SMoV)is one of the most common viruses infecting strawberries,causing losses to fruit yield and quality.In this study,165 strawberry leaf samples were collected from six provinces of China,46 of which tested positive for SMoV.The complete genome sequences of 11 SMoV isolates were obtained from Liaoning(DGHY3,DGHY16-2,DGHY17,DGHY20-2,DGHY21,DGHY26-2),Shandong(SDHY1,SDHY5,SDHY31-2,SDHY33-2),and Beijing(BJMX7).The RNA1 and RNA2 nucleotide identities between the 11 Chinese isolates were 95.4-99.3%and 96.3-99.6%,respectively,and they shared 78.4-96.6%and 84.8-93.5%identities with the available SMoV isolates in GenBank.Recombination analysis revealed that Chinese isolate SDHY33-2 and Canadian isolates Ontario and Simcoe were recombinants,and recombination events frequently occurred in the 3’UTR of SMoV.Phylogenetic analysis showed that in an RNA1 tree,most Chinese isolates clustered into the same group while isolate DGHY17 clustered into another group together with Czech isolate C and three Canadian isolates.In an RNA2 tree,all Chinese isolates clustered into a single group.The phylogenetic analysis based on nucleotide sequences was consistent with the results based on coat protein(CP)and RNA-dependent RNA polymerase(RdRp).Further evolutionary analysis indicated that negative selection drives SMoV evolution,and gene flow plays a major role in genetic differentiation.Additionally,reassortment and recombination also influence the evolution of SMoV.To our knowledge,this is the first report of the complete genome of SMoV isolates from China and a detailed analysis of the SMoV population structure. | LINGJIAO FAN CHENGYONG HE MENGMENG WU DEHANG GAO ZHENFEI DONG SHENGFAN HOU ZEKUN FENG HONGQING WANG | 2021 | BIOCELL2021，45，4： | 1 |
| 10 | Continuous culture of urine-derived bladder cancer cells for precision medicine显示文摘Dear Editor,Bladder cancer is the most common type of genitourinary cancer in China,with an estimated 80,500 new cases and 32,900 related deaths in 2015 alone(Chen et al.,2016).Unlike many other cancers,there has been no significant improvement in survival rates for bladder cancer over the last three decades.Specific treatment regimens for bladder cancer and their efficacy vary depending not only on clinical stages,but also on associated risk factors and other personal clinical characteristics.Patients with non-muscle invasive bladder cancer(NMIBC)have a high 5-year recurrence rate of 60%-70%(Berdik,2017)and those with muscle invasive bladder cancer(MIBC)has a relatively poor prognosis with approximately 65%risk of death within 5-year follow-up(Kamat et al.,2016).Therefore,there is an urgent need to develop models for bladder cancer to screen for rational treatment strategies by personalized medicine to improve the clinical assessment and treatment of bladder cancer. | Shuai Jiang Jiaqi Wang Chen Yang Renke Tan Jun Hou Yuan Shi Huihui Zhang Shiyu Ma Jianan Wang Mengmeng Zhang George Philips Zengxia Li Jian Ma Wanjun Yu Guohua Wang Yuanming Wu Richard Schlegel Huina Wang Shanbo Cao Jianming Guo Xuefeng Liu Yongjun Dang | 2019 | Protein & Cell2019，10，12： | 1 |
| 11 | The genomic and bulked segregant analysis of Curcuma alismatifolia revealed its diverse bract pigmentation显示文摘Compared with most flowers where the showy part comprises specialized leaves(petals)directly subtending the reproductive structures,most Zingiberaceae species produce showy“flowers”through modifications of leaves(bracts)subtending the true flowers throughout an inflorescence.Curcuma alismatifolia,belonging to the Zingiberaceae family,a plant species originating from Southeast Asia,has become increasingly popular in the flower market worldwide because of its varied and esthetically pleasing bracts produced in different cultivars.Here,we present the chromosome-scale genome assembly of C.alismatifolia“Chiang Mai Pink”and explore the underlying mechanisms of bract pigmentation.Comparative genomic analysis revealed C.alismatifolia contains a residual signal of whole-genome duplication.Duplicated genes,including pigment-related genes,exhibit functional and structural differentiation resulting in diverse bract colors among C.alismatifolia cultivars.In addition,we identified the key genes that produce different colored bracts in C.alismatifolia,such as F3′5'H,DFR,ANS and several transcription factors for anthocyanin synthesis,as well as chlH and CAO in the chlorophyll synthesis pathway by conducting transcriptomic analysis,bulked segregant analysis using both DNA and RNA data,and population genomic analysis.This work provides data for understanding the mechanism of bract pigmentation and will accelerate breeding in developing novel cultivars with richly colored bracts in C.alismatifolia and related species.It is also important to understand the variation in the evolution of the Zingiberaceae family. | Xuezhu Liao Yuanjun Ye Xiaoni Zhang Dan Peng Mengmeng Hou Gaofei Fu Jianjun Tan Jianli Zhao Rihong Jiang Yechun Xu Jinmei Liu Jinliang Yang Wusheng Liu Luke RTembrock Genfa Zhu Zhiqiang Wu | 2022 | aBIOTECH2022，3，3： | 1 |
| 12 | The effects of RFRP-3, the mammalian ortholog of GnIH, on the female pig reproductive axis in vitro显示文摘 | Xun Li Juan Su Rui Fang Lucheng Zheng Ruipeng Lei Xiaoye Wang Zhihai Lei Mengmeng Jin Yang Jiao Yuanlong Hou Tingting Guo Zhiyu Ma | 2013 | Molecular and Cellular Endocrinology (-)2013，，1： | 1 |
| 13 | Synthesis of high oil-absorption resins of poly(methyl-methaery late-butyl methacry- late) by suspended emulsion polymerization显示文摘 | Ji Naiyi Chen Hou Yu Mengmeng | 2011 | Polymers for Advanced Technologies2011，22，12： | 1 |
| 14 | Fabrication and luminescence properties of one-dimensional ZnAl 2 O 4 and ZnAl 2 O 4 : A 3+ (A=Cr, Eu, Tb) microfibers by electrospinning method显示文摘 | Chong Peng Guogang Li Dongling Geng Mengmeng Shang Zhiyao Hou Jun Lin | 2012 | Materials Research Bulletin2012，，11： | 1 |
| 15 | Synthesis of high oil- absorption resins of poly(methyl methacrylate-butyl methacrylate) by suspended emulsion polymerization显示文摘 | Ji Naiyi Chen Hou Yu Mengmeng | 2011 | Polym Adv Technol2011，22，12： | 1 |
| 16 | Significantly enhanced photocatalytic hydrogen production performance of g-C_(3)N_(4)/CNTs/CdZnS with carbon nanotubes as the electron mediators显示文摘The electron mediator can effectively improve the performance of the direct Z-scheme heterojunction photocatalysts. However, it is still a great challenge to select cheap and efficient electron mediators and to design them into the Z-scheme photocatalytic system. In the present paper, the g-C_(3)N_(4)/CNTs/CdZnS Z-scheme photocatalyst was prepared using carbon nanotubes(CNTs) as the electron mediators, and its photocatalytic hydrogen production performance was studied. Compared with single-phase g-C_(3)N_(4),CdZnS and biphasic g-C_(3)N_(4)/CdZnS photocatalysts, the photocatalytic hydrogen production performance of the prepared g-C_(3)N_(4)/CNTs/CdZnS has been significantly enhanced. Meanwhile, g-C_(3)N_(4)/CNTs/CdZnS possesses very good photocatalytic hydrogen production stability. The enhanced photocatalytic hydrogen production performance of g-C_(3)N_(4)/CNTs/CdZnS is attributed to the fact that CNTs, as an electron mediator,can accelerate the recombination of the photogenerated holes in the valence band of g-C_(3)N_(4) and the photogenerated electrons in the conduction band of CdZnS, which makes the g-C_(3)N_(4)/CNTs/CdZnS Zscheme photocatalyst be easier to escape the photogenerated electrons, increases the concentration of the photogenerated carriers and prolongs the lifetime of the photogenerated carriers. This work provides a theoretical basis for the further development and design of CNTs as the intermediate electron mediator of the Z-scheme heterojunction photocatalyst. | Chang Feng Zhuoyuan Chen Jiangping Jing Mengmeng Sun Jing Tian Guiying Lu Li Ma Xiangbo Li Jian Hou | 2021 | Journal of Materials Science & Technology2021，，21： | 0 |
| 17 | Determination of Double Beta Decay Half-Life of 136Xe with the PandaX-4T Natural Xenon Detector显示文摘Precise measurement of two-neutrino double beta decay(DBD)half-life is an important step for the searches of Majorana neutrinos with neutrinoless double beta decay.We report the measurement of DBD half-life of 136xe using the Pandax-4T dual-phase Time Projection Chamber(TPC)with 3.7-tonne natural xenon and the first 94.9-day physics data release. | Lin Si Zhaokan Cheng Abdusalam Abdukerim Zihao Bo Wei Chen Xun Chen Yunhua Chen Chen Cheng Yunshan Cheng Xiangyi Cui Yingjie Fan Deqing Fang Changbo Fu Mengting Fu Lisheng Geng Karl Giboni Linhui Gu Xuyuan Guo Ke Han Changda He Jinrong He Di Huang Yanlin Huang Zhou Huang Ruquan Hou Xiangdong Ji Yonglin Ju Chenxiang Li Jiafu Li Mingchuan Li Shu Li Shuaijie Li Qing Lin Jianglai Liu Xiaoying Lu Lingyin Luo Yunyang Luo Wenbo Ma Yugang Ma Yujun Mao Yue Meng Nasir Shaheed Xiaofeng Shang Xuyang Ning Ningchun Qi Zhicheng Qian Xiangxiang Ren Changsong Shang Guofang Shen Wenliang Sun Andi Tan Yi Tao Anqing Wang Meng Wang Qiuhong Wang Shaobo Wang Siguang Wang Wei Wang Xiuli Wang Zhou Wang Yuehuan Wei Mengmeng Wu Weihao Wu Jingkai Xia Mengjiao Xiao Xiang Xiao Pengwei Xie Binbin Yan Xiyu Yan Yong Yang Chunxu Yu Jumin Yuan Ying Yuan Zhe Yuan Dan Zhang Minzhen Zhang Peng Zhang Shibo Zhang Shu Zhang Tao Zhang Li Zhao Qibin Zheng Jifang Zhou Ning Zhou Xiaopeng Zhou Yong Zhou | 2023 | Research2023，，2： | 0 |
| 18 | Efficient production of 2′-fucosyllactose in unconventional yeast Yarrowia lipolytica显示文摘2′-Fucosyllactose(2′-FL)has great application value as a nutritional component and the whole cell biosynthesis of 2′-FL has become the focus of current research.Yarrowia lipolytica has great potential in oligosaccharide synthesis and large-scale fermentation.In this study,systematic engineering of Y.lipolytica for efficient 2′-FL production was performed.By fusing different protein tags,the synthesis of 2′-FL was optimized and the ubiquitin tag was demonstrated to be the best choice to increase the 2′-FL production.By iterative integration of the related genes,increasing the precursor supply,and promoting NADPH regeneration,the 2′-FL synthesis was further improved.The final 2′-FL titer,41.10 g/L,was obtained in the strain F5-1.Our work reports the highest 2′-FL production in Y.lipolytica,and demonstrates that Y.lipolytica is an efficient microbial chassis for the synthesis of oligosaccharides. | Yan Zhang Xuejing Zhang Haiyan Liu Jin Hou Mengmeng Liu Qingsheng Qi | 2023 | Synthetic and Systems Biotechnology2023，8，4： | 0 |
| 19 | Temperature/pH dual-responsive reversible morphology evolution of block copolymer microparticles under three-dimensional confinement显示文摘Development of block copolymer(BCP)microparticles with switchable morphology in response to external stimuli is important for exploiting new intelligent materials.In this work,thermo/p H dual-responsive nanoparticles(NPs)were employed as a cosurfactant to modulate the self-assembly morphology of polystyrene-b-poly(2-vinylpyridine)(PS-b-P2VP)microparticles within confined emulsion droplets.The co-surfactant was synthesized by grafting poly(acrylic acid)-b-poly(N-isopropylacrylamide)onto the surface of Fe3O4NP.The introduction of the dual-responsive co-surfactant enabled thermo/p H dual-responsive reversible morphology transition of the PS-b-P2VP microparticles by tailoring the hydrophobicity and interfacial affinity of the cosurfactant.By using this strategy,the thermo-inert PS-b-P2VP self-assembled into pupa-like microparticles at T=10℃ and p H7.5,which could transform into tulip-like microparticles when T was increased to 50℃.When the p H value was increased to 11,the pupa-like particles turned into onion-like microparticles although the PS-b-P2VP was inert to alkali.However,the pupa-like microparticles remained unchanged when both T and p H were simultaneously increased.The PAA-b-PNIPAM-grafted Fe3O4NP surfactants showed obvious advantages over the linear PAA-b-PNIPAM surfactants in modulating the morphology transition,since the linear PAA-b-PNIPAM could not induce the reversible shape transition of microparticles.Our work provides an efficient strategy to achieve reversible shape transformation of BCP microparticles while the internal phase structure is preserved,which may be utilized to switch the structural color properties of BCP microparticles. | Mengmeng Zhang Zaiyan Hou Simeng Liu Guoqiang Han Meirong Tian Jintao Zhu Jiangping Xu | 2023 | Science China Chemistry2023，66，12： | 0 |
| 20 | Titanium-promoted Intramolecular Photoenolization/Diels-Alder Reaction to Construct Polycyclic Terpenoids: Formal Synthesis of Mycoleptodiscin A显示文摘Summary of main observation and conclusion A titanium-promoted intramolecular photoenolization/Diels-Alder (PEDA) reaction was developed to construct the core skeleton of aromatic polycyclic terpenoids bearing an all-carbon quaternary center on the benzylic position. Titanium(Ⅳ) isopropoxide [Ti(Oi-Pr)4] plays a key role during the photo cycloaddition, which may help to accelerate the in teraction between dienophile and the stereo-hi ndered diene species as well as control the diastereoselectivity. This photolysis provides a new solution for the stereospecific formation core structures of aromatic abietane diterpenoids and sesquiterpenoids, which have multiple functional groups for the further transformations. As a synthetic application, it was successfully used in the synthesis of indolosesquiterpenoid mycoleptodiscin A. | Dongsheng Xue Mengmeng Xu Chaoying Zheng Baochao Yang Min Hou Haibing He Shuanhu Gao | 2019 | Chinese Journal of Chemistry2019，37，2： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员