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| 1 | NEAT1/miR-140-3p/MAPK1 mediates the viability and survival of coronary endothelial cells and affects coronary atherosclerotic heart disease显示文摘Studies have shown that long non-coding RNAs(lncRNA)play critical roles in coronary atherosclerotic heart disease(CAD).However,the function of lncRNA nuclear enriched abundant transcript 1(NEAT1)in CAD is unclear.In this study,we aimed to investigate the functions of lncRNA NEAT1 in CAD.RTPCR and western blot analysis were carried out to examine the expressions of related RNAs.Colony formation assay,cell proliferation assay,apoptosis assay,and dual-luc讦erase reporter assay were con ducted to investigate the abilities of colony migration,cell proliferatio n,apoptosis,and targeting.The results showed that NEAT1 was up-regulated in CAD blood samples and in human coronary endothelial cells(HCAECs).Transfection of pcNEATI sign讦icantly inhibited the survival rate of HCAECs and induced apoptosis of HCAECs.MiR-140-3p was down-regulated in HCAECs.NEAT1 directly targeted miR-140-3p,and the expression of miR-140-3p was inversely correlated with the expression of NEAT1 in CAD patients.In addition,co-transfection of NEAT1 with miR-140-3p mimic reversed the effect of pcNEATI on cell viability and apoptosis,mitogenactivated protein kinase 1(MAPK1)was proved to be a target gene of miR-140-3p,and the miR-140-3p mimic was shown to reduce the expression of MAPK1 in HCAECs.pcNEATI sigrdficantly increased the expression level of MAPK1,while shNEATI significantly reduced the expression level of MAPK1.Our results revealed that lncRNA NEAT1 increased cell viability and inhibited CAD cell apoptosis possibly by activating the miR-140-3p/MAPK1 pathway,and lncRNA NEAT1 might serve as a potential therapeutic target for CAD. | Hui Zhang Ningning Ji Xinyan Gong Shimao Ni Yu Wang | 2020 | Acta Biochimica et Biophysica Sinica2020，52，9： | 9 |
| 2 | Integration of A Deep Learning Classifier with A Random Forest Approach for Predicting Malonylation Sites显示文摘As a newly-identified protein post-translational modification, malonylation is involved in a variety of biological functions. Recognizing malonylation sites in substrates represents an initial but crucial step in elucidating the molecular mechanisms underlying protein malonylation. In this study, we constructed a deep learning(DL) network classifier based on long short-term memory(LSTM) with word embedding(LSTMWE) for the prediction of mammalian malonylation sites.LSTMWEperforms better than traditional classifiers developed with common pre-defined feature encodings or a DL classifier based on LSTM with a one-hot vector. The performance of LSTMWE is sensitive to the size of the training set, but this limitation can be overcome by integration with a traditional machine learning(ML) classifier. Accordingly, an integrated approach called LEMP was developed, which includes LSTMWEand the random forest classifier with a novel encoding of enhanced amino acid content. LEMP performs not only better than the individual classifiers but also superior to the currently-available malonylation predictors. Additionally, it demonstrates a promising performance with a low false positive rate, which is highly useful in the prediction application. Overall, LEMP is a useful tool for easily identifying malonylation sites with high confidence.LEMP is available at http://gffzz2e6a800e5edb4052hcwoo5ffnxn9v65c0.ffgz.tsg.suse.edu.cn/lemp. | Zhen Chen Ningning He Yu Huang Wen Tao Qin Xuhan Liu Lei Li | 2018 | Genomics, Proteomics & Bioinformatics2018，16，6： | 4 |
| 3 | Interactions between bubble and particles of key minerals of diasporic bauxite through the extended DLVO theory显示文摘The flotation of diasporic bauxite is to separate diaspore(valuable mineral)from aluminosilicate minerals(gangue minerals,mainly including kaolinite,illite and pyrophyllite),and the microscopic interaction force between the two types of minerals and air bubbles determines the separation efficiency.In this paper,based on the extended Derjaguin-Landau-Verwey-Overbeek(DLVO)theory,the van der Waals,electrostatic and hydrophobic interaction between particles of the four minerals mentioned above and air bubbles in collectorless solution were calculated first,and then diaspore and kaolinite were taken as examples to analyze the influence of various factors such as electrolyte concentration,mineral particle size,air bubble size,collector type(dodecylamine hydrochloride(DAH)and sodium oleate(NaOL))and concentration,and pulp pH on the interactions between the particles of valuable mineral and gangue minerals and air bubbles.The results showed that the total extended DLVO interactions between the four minerals and air bubbles were repulsive in most cases in collectorless solution.The increase in electrolyte concentration reduced the interaction force or even changed the direction of the force under certain circumstances.The addition of DAH and NaOL can reduce the adhesion energy barrier of kaolinitebubble and diaspore-bubble respectively.Each type of minerals exhibited a specific interface interaction response with air bubbles in each collector with different pH values.The research results have theoretical guiding significance for the optimization and directional control of diasporic bauxite flotation conditions. | Ningning Zhang Tian Pang Rui Han Songjiang Chen Zhen Li Yuexian Yu Zhongyu Shi Lijun Liu Jinzhou Qu Anning Zhou | 2022 | International Journal of Mining Science and Technology2022，32，1： | 3 |
| 4 | Significance of combined detection of LunX mRNA and tumor markers in diagnosis of lung carcinoma显示文摘Objective:To evaluate the significance of combined detection of LunX mRNA,carcinoembryonic antigen(CEA),neuron-specific enolase(NSE),and cytokeratin 21-1 fragment(CYFRA21-1) in clinical diagnosis of lung carcinoma.Methods:Based on the quantitative RT-PCR and chemiluminescence immunoassay,the expression levels of LunX mRNA,CEA,NSE,and CYFRA21-1 in 113 patients with lung carcinoma(case group) and 30 healthy participants(control group) were detected.Meantime,the sensitivity,specificity,and accuracy of the combination detection were also explored.Results:The positive rates of LunX mRNA in peripheral blood and CEA,NSE,and CYFRA21-1 in serum were significantly higher in case group than those in control group(χ2=17.295,16.825,19.148,and 17.450;P<0.05).There was no statistical significance when positive rate of LunX mRNA was evaluated among different pathological types(χ2=0.047,P>0.05).The positive rate of LunX mRNA in stage Ⅰ+Ⅱ,Ⅲ,and Ⅳ had a significantly increasing tendency(χ2=10.565,32.462,P<0.05).The positive rate of CYFRA21-1 was highest in squamous carcinoma(78.5%),the positive rate of NSE was highest in small cell carcinoma(86.7%),and the positive rate of CEA wag highest in lung adenocarcinoma(80.4%).The sensitivity and accuracy of the combination detection were 91.1% and 88.1%,respectively.Conclusions:The combined detection of LunX mRNA and tumor markers(TMs) including CEA,NSE,and CYFRA21-1 in peripheral blood is helpful to increase the diagnostic accuracy of lung cancer.Also,it can inform the pathological typing of lung carcinoma. | Hui Yu Xiuying Huang Zhihua Zhu Yi Hu Wei Ou Lanjun Zhang Ningning Zhou | 2014 | Chinese Journal of Cancer Research2014，26，1： | 3 |
| 5 | Regulation of Leaf Longevity by DML3-Mediated DNA Demethylation显示文摘Leaf senescence is driven by the expression of senescence-associated genes(SAGs).Development-specific genes often undergo DNA demethylation in their promoter and other regions,which regulates gene expression.Whether and how DNA demethylation regulates the expression of SAGs and thus leaf senescence remain elusive.Whole-genome bisulfite sequencing(WGBS)analyses of wild-type(WT)and demeter-like 3(dml3)Arabidopsis leaves at three developmental stages revealed hypermethylation during leaf senescence in dml3 compared with WT,and 20556 differentially methylated regions(DMRs)were identified by comparing the methylomes of dml3 and WT in the CG,CHG,and CHH contexts.Furthermore,we identified that 335 DMR-associated genes(DMGs),such as NAC016 and SEN1,are upregulated during leaf senescence,and found an inverse correlation between the DNA methylation levels(especially in the promoter regions)and the transcript abundances of the related SAGs in WT.In contrast,in dml3 the promoters of SAGs were hypermethylated and their transcript levels were remarkably reduced,and leaf senescence was significantly delayed.Collectively,our study unraveled a novel epigenetic regulatory mechanism underlying leaf senescence in which DML3 is expressed at the onset of and during senescence to demethylate promoter,gene body or 3'UTR regions to activate a set of SAGs. | Lu Yuan Dan Wang Liwen Cao Ningning Yu Ke Liu Yongfeng Guo Susheng Gan Liping Chen | 2020 | Molecular Plant2020，13，8： | 3 |
| 6 | DEM simulation of particle flow and separation in a vibrating flip-flow screen显示文摘Vibrating flip-flow screens(VFFS)with stretchable polyurethane sieve mats have been widely used in screening fine-grained materials in recent years.In this work,the discrete element method(DEM)is used to study the screening process in VFFS to explain particle flow and separation behavior at the particle scale.Unlike traditional vibrating screens,for VFFS,the amplitude response of each point on the elastic sieve mat is different everywhere.This study measures the kinematics of the elastic sieve mat under different conditions such as different stretched lengths and material loads.To establish the elastic sieve mat model in a DEM simulation,the continuous elastic sieve mat is discretized into multiple units,and the displacement signal of each unit tested is analyzed by Fourier series.The Fourier series analysis results of each unit are used as the setting parameters for motion.In this way,the movement of the elastic sieve mat is approximately simulated,and a DEM model of VFFS is produced.Through the simulation,the flow and separation of different-sized particles in VFFS are studied,and the reasonability of the simulation is verified by a pilot-scale screening experiment.The present study demonstrates the potential of the DEM method for the analysis of screening processes in VFFS. | Chi Yu Dongdong Lin Ningning Xu Xinwen Wang Kunwei Pu Zhenghao Wang Guofeng Zhao Runhui Geng Sanpeng Gong | 2023 | Particuology2023，，2： | 2 |
| 7 | 定量分析乳腺癌术后化疗前外周血多药耐药基因的检测及其临床意义的研究(英文)显示文摘Objective: To explore expressions of multidrug resistance gene (MDR) product P170 and peripheral blood MDR1 mRNA in breast cancer tissue and their clinical significances. Methods: Fluorescent quantitative RT-PCR and immunohis-tochemistry were used to detect peripheral blood MDR1 mRNA before chemotherapy in breast cancer tissue samples from 32 cases with P170 positive (trial group) and 11 cases with P170 negative (control group). Results: There were 14 cases (43.75%) peripheral blood MDR1 mRNA positive and 18 cases (56.25%) negative in 32 cases P170 positive breast cancer pa-tients, while there were 6 cases (54.55%) peripheral blood MDR1 mRNA positive and 5 cases (45.45%) negative in 11 cases P170 negative breast cancer patients; there wasn't a significance difference with peripheral blood MDR1 mRNA express rate in P170 positive (43.75%) group and P170 negative (54.55%) group (χ2 = 0.383, P > 0.05). There were 12 cases (37.50%) Her-2 positive and 20 cases (62.50%) negative in P170 positive group; there were 2 cases (18.18%) Her-2 positive and 9 cases (81.82%) negative in P170 negative group; there wasn't a significance difference with Her-2 positive rate in P170 posi-tive group (37.50%) and P170 negative (18.18%) group (χ2 = 1.391, P > 0.05). There were 13 cases (40.63%) ER positive and 19 cases (59.37%) negative in P170 positive group; there were 7 cases (63.64%) ER positive and 4 cases (36.36%) negative in P170 negative group; there wasn't a significance difference with ER positive rate in P170 positive group (40.63%) and P170 negative (63.64) group (χ2 = 1.742, P > 0.05). There were 20 cases (62.50%) PR positive and 12 cases (37.50%) negative in P170 positive group; there were 4 cases (36.36%) PR positive and 7 cases (63.64%) negative in P170 negative group; there wasn't a significance difference with PR positive rate in P170 positive group (62.50%) and P170 negative (36.36%) group (χ2 = 2.267, P > 0.05). There were 9 cases (28.12%) with lymphaden metastasis and 23 cases (71.88%) without lymphaden metastasis in P170 positive group; there were 5 cases (45.45%) with lymphaden metastasis and 6 cases (54.55%) without lymphaden metastasis in P170 negative group; there wasn't a significance difference with lymphaden metastasis rate in P170 positive group (28.12%) and P170 negative (45.45%) group (χ2 = 1.120, P > 0.05). Conclusion: 1) There were possibly MDR expressing infrequently in carcinoma tissue and peripheral blood before chemotherapy. 2) FQ-RT-PCR for detecting of periph-eral blood MDR1 mRNA is special, sensitive and reliable. It can be used as new molecular biology diagnostic maker dynamic detecting peripheral blood MDR1 mRNA for regulating chemotherapy proposal and elevating chemotherapy effect. | Xinmei Wang Liang Li Qjng Cuix Muxin Wang Yu Ding Tongfu Jia Jianzhao Li Ningning Feng Anjing Zuo Yanchang Feng | 2008 | The Chinese-German Journal of Clinical Oncology2008，7，11： | 2 |
| 8 | Vancomycin pretreatment attenuates acetaminophen-induced liver injury through 2-hydroxybutyric acid显示文摘Liver injury caused by acetaminophen(AP)overdose is a leading public health problem.Although APinduced liver injury is well recognized as the formation of N-acetyl-p-benzoquinone(NAPQI),a toxic metabolite of AP,resulting in cell damage,emerging evidence indicates that AP-induced liver injury is also associated with gut microbiota.However,the gut microbiota-involved mechanism remains largely unknown.In our study,we found that vancomycin(Vac)pretreatment(100 mg/kg,twice a day for 4 days)attenuated AP-induced liver injury,altered the composition of gut microbiota,and changed serum metabolic profile.Moreover,we identified Vac pretreatment elevated cecum and serum 2-hydroxybutyric acid(2-HB),which ameliorated AP-induced cell damage and liver injury in mice by reducing AP bioavailability and elevating GSH levels.Our current results revealed the novel role of 2-HB in protecting AP-induced liver injury and add new evidence for gut microbiota in affecting AP toxicity. | Ningning Zheng Yu Gu Ying Hong Lili Sheng Linlin Chen Feng Zhang Jie Hou Weidong Zhang Zean Zhang Wei Jia Houkai Li | 2020 | Journal of Pharmaceutical Analysis2020，10，6： | 1 |
| 9 | Label-free and sequence-specific DNA detection down to a picomolar level with carbon nanotubes as support for probe DNA显示文摘 | Ningning Zhu Yuqing Lin Ping Yu | 2009 | Analytica Chimica Acta2009，650，： | 1 |
| 10 | Pharmacogenetic assessment of clinical outcome in patients with metastatic breast cancer treated with docetaxel plus capecitabine显示文摘 | Ningning Dong Jing Yu Chaoying Wang Xiaohui Zheng Zheng Wang Lijun Di Guohong Song Budong Zhu Li Che Jun Jia Hanfang Jiang Xinna Zhou Xiaoli Wang Jun Ren | 2012 | Journal of Cancer Research and Clinical Oncology2012，，7： | 1 |
| 11 | An mRNA vaccine encoding Chikungunya virus E2-E1 protein elicits robust neutralizing antibody responses and CTL immune responses显示文摘Arthropod-borne chikungunya virus(CHIKV)infection can cause a debilitating arthritic disease in human.However,there are no specific antiviral drugs and effective licensed vaccines against CHIKV available for clinical use.Here,we developed an m RNA-lipid nanoparticle(m RNA-LNP)vaccine expressing CHIKV E2-E1 antigen,and compared its immunogenicity with soluble recombinant protein s E2-E1 antigen expressed in S2 cells.For comparison,we first showed that recombinant protein antigens mixed with aluminum adjuvant elicit strong antigenspecific humoral immune response and a moderate cellular immune response in C57BL/6 mice.Moreover,s E2-E1vaccine stimulated 12-23 folds more neutralizing antibodies than s E1 vaccine and s E2 vaccine.Significantly,when E2-E1 gene was delivered by an m RNA-LNP vaccine,not only the better magnitude of neutralizing antibody responses was induced,but also greater cellular immune responses were generated,especially for CD8+T cell responses.Moreover,E2-E1-LNP induced CD8~+T cells can perform cytotoxic effect in vivo.Considering its better immunogenicity and convenience of preparation,we suggest that more attention should be placed to develop CHIKV E2-E1-LNP m RNA vaccine. | Ningning Ge Jin Sun Zhihua Liu Jiayi Shu Huimin Yan Zhihua Kou Yu Wei Xia Jin | 2022 | Virologica Sinica2022，37，2： | 1 |
| 12 | Maintenance of species boundaries in three sympatric Ligularia(Senecioneae,Asteraceae)species显示文摘The key process in speciation concerns the formation and maintenance of reproductive isolating barriers between diverging lineages.Although species boundaries are frequently investigated between two species across many taxa,reproductive isolating barriers among multiple species(>2)that would represent the most common phenomenon in nature,remain to be clarified.Here,we use double digest restriction-site associated DNA(ddRAD)sequencing to examine patterns of hybridization at a sympatric site where three Ligularia species grow together and verify whether those patterns contribute to the maintenance of boundaries among species.The results based on the RAD SNP datasets indicated hybridization Ligularia cyathiceps?L.duciformis and L.duciformis?L.yunnanensis were both restricted to F1s plus a few first-generation backcrosses and no gene introgression were identified,giving rise to strong reproductive isolation among hybridizing species.Moreover,hybrid swarm simulation,using HYBRIDLAB,indicated the RAD SNP datasets had sufficient discriminatory power for accurate hybrid detection.We conclude that parental species show strong reproductive isolation and they still maintain species boundaries,which may be the key mechanism to maintain species diversity of Ligualria in the eastern Qinghai-Tibetan Plateau and adjacent areas.Moreover,this study highlights the effectiveness of RAD sequencing in hybridization studies. | Ningning Zhang Yongpeng Ma Ryan A.Folk Jiaojun Yu Yuezhi Pan Xun Gong | 2018 | Journal of Integrative Plant Biology2018，60，10： | 1 |
| 13 | Down-staging depth score could be a survival predictor for locally advanced gastric cancer patients after preoperative chemoradiotherapy显示文摘Objective:The predictive effect of preoperative chemoradiotherapy(CRT)is low and difficult in guiding individualized treatment.We examined a surrogate endpoint for long-term outcomes in locally advanced gastric cancer patients after preoperative CRT.Methods:From April 2012 to April 2019,95 patients with locally advanced gastric cancer who received preoperative concurrent CRT and who were enrolled in three prospective studies were included.All patients were stage T_(3/4) N_(+).Local control,distant metastasis-free survival(DMFS),disease-free survival(DFS)and overall survival(OS)were evaluated.Clinicopathological factors related to long-term prognosis were analyzed using univariate and multivariate analyses.The down-staging depth score(DDS),which is a novel method of evaluating CRT response,was used to predict long-term outcomes.Results:The median follow-up period for survivors was 30 months.The area under the curve(AUC)of the receiver operating characteristic(ROC)curve predicted by the DDS was 0.728,which was better than the pathological complete response(pCR),histological response and ypN0.Decision curve analysis further affirmed that DDS had the largest net benefit.The DDS cut-off value was 4.pCR and ypN0 were associated with OS(P=0.026 and 0.049).Surgery and DDS are correlated with DMFS,DFS and OS(surgery:P=0.001,<0.001 and<0.001,respectively;and DDS:P=0.009,0.013 and 0.032,respectively).Multivariate analysis showed that DDS was an independent prognostic factor of DFS(P=0.021).Conclusions:DDS is a simple,short-term indicator that was a better surrogate endpoint than pCR,histological response and ypN0 for DFS. | Ning Li Xin Wang Yuan Tang Dongbin Zhao Yihebali Chi Lin Yang Liming Jiang Jun Jiang Jinming Shi Wenyang Liu Hua Ren Hui Fang Yu Tang Bo Chen Ningning Lu Hao Jing Shunan Qi Shulian Wang Yueping Liu Yongwen Song Yexiong Li Jing Jin | 2021 | Chinese Journal of Cancer Research2021，33，4： | 1 |
| 14 | Optimization of antioxidant hydrolysate production from flying squid muscle protein using response surface methodology显示文摘 | Xubo Fang Ningning Xie Xiaoe Chen Hui Yu Jing Chen | 2012 | Food and Bioproducts Processing2012，，4： | 1 |
| 15 | Mass transfer techniques for large-scaleand high-density microLED arrays显示文摘Inorganic-based micro light-emitting diodes (microLEDs) offer more fascinating properties and unique demands in next-generation displays. However, the small size of the microLED chip (1–100 µm) makes it extremely challenging for high efficiency and low cost to accurately, selectively, integrate millions of microLED chips. Recent impressive technological advances have overcome the drawbacks of traditional pick-and-place techniques when they were utilized in the assembly of microLED display, including the most broadly recognized laser lift-off technique, contact micro-transfer printing (µTP) technique, laser non-contact µTP technique, and self-assembly technique. Herein, we firstly review the key developments in mass transfer technique and highlight their potential value, covering both the state-of-the-art devices and requirements for mass transfer in the assembly of the ultra-large-area display and virtual reality glasses. We begin with the significant challenges and the brief history of mass transfer technique, and expand that mass transfer technique is composed of two major techniques, namely, the epitaxial Lift-off technique and the pick-and-place technique. The basic concept and transfer effects for each representative epitaxial Lift-off and pick-and-place technique in mass transfer are then overviewed separately. Finally, the potential challenges and future research directions of mass transfer are discussed. | Furong Chen Jing Bian Jinlong Hu Ningning Sun Biao Yang Hong Ling Haiyang Yu Kaixin Wang Mengxin Gai Yuhang Ma YongAn Huang | 2022 | International Journal of Extreme Manufacturing2022，4，4： | 1 |
| 16 | Effects of Dihydromyricetin Extracted from Ampelopsis grossedentata on Visceral Organ Indexes and Intestinal Digestive Enzyme Activities of Chickens显示文摘To provide a reference basis for reasonable development and utilization of Ampelopsis grossedentata resource and its application in production,we added 0.025%,0.05% and 0.1% dihydromyricetin(DMY)extracted from A.grossedentata into the basal diets of Yaoshan chickens,and studied the effects of DMY on visceral organ indexes and digestive enzyme activities of 40-day-old healthy Yaoshan chickens.Supplementation of DMY in basal diets influenced visceral organ weight,organ index,length and digestive enzyme activity of small intestine of chickens.Among them,0.05% DMY was the most appropriate volume for supplementation.Compared with control group(without DMY),0.05% DMY reduced lung weight and index(P>0.05),significantly reduced liver weight(P<0.05),extremely reduced stomach weight and stomach index(P<0.01),increased activity of amylase in small intestine(P>0.05),significantly increased length of small intestine and activity of protease(P<0.05).Supplementation of 0.05% DMY reduced the visceral organ weight and organ index,enhanced digestion and absorption ability of gastrointestinal tract,thereby improving feed utilization rate and promoting the growth of chickens. | Dai Qing Yu Jianping Yu Lina Han Ningning Xu Yuan Zhao Hui | 2016 | Animal Husbandry and Feed Science2016，8，3： | 1 |
| 17 | Isolation and characterization of microsatellite loci in tiger frog (Hoplobatrachus rugulosus)显示文摘 | Chen Shao Yu Wang Ningning Qiao | 2009 | Conservation Genetics2009，，5： | 1 |
| 18 | Influence of traditional Chinese medicines on the in vivo metabolism of lopinavir/ritonavir based on UHPLC-MS/MS analysis显示文摘A fast,reliable,and cost-effective liquid chromatography-tandem mass spectrometry method was established to determine the effects of the traditional Chinese medicine employed to treat coronavirus disease 2019,namely,Lianhua Qingwen granules,Huoxiang Zhengqi capsules,Jinhua Qinggan granules,Shufeng Jiedu capsules,and Angong Niuhuang pills,on the pharmacokinetics of lopinavir/ritonavir in rats.Blood samples were prepared using the protein precipitation method and atazanavir was selected as the internal standard(IS).Separation was performed on an Agilent ZORBAX eclipse plus C18(2.1 mm×50 mm,1.8 μm)column using acetonitrile and water containing 0.1%formic acid as the mobile phase for gradient elution.The flow rate was 0.4 mL/min and the injection volume was 2 μL.Agilent Jet Stream electrospray ionization was used for mass spectrometry detection under positive ion multiple reaction monitoring mode at a transition of m/z 629.3→447.3 for lopinavir,m/z 721.3→296.1 for ritonavir,and m/z 705.4→168.1 for the IS.The method showed good linearity in the concentration range of 25→2500 ng/mL(r=0.9981)for lopinavir and 5e500 ng/mL(r=0.9984)for ritonavir.The intra-day and inter-day precision and accuracy were both within±15%.Items,such as dilution reliability and residual effect,were also within the acceptable limits.The method was used to determine the effects of five types of traditional Chinese medicines on the pharmacokinetics of lopinavir/ritonavir in rats.The pharmacokinetic results showed that the half-life of ritonavir in the groups administered Lianhua Qingwen granules and Huoxiang Zhengqi capsules combined with lopinavir/ritonavir was prolonged by approximately 1.5-to 2-fold relative to that in the control group.Similarly,the pharmacokinetic parameters of lopinavir were altered.Overall,the results of this study offer important theoretical parameters for the effective clinical use of five types of traditional Chinese medicines combined with lopinavir/ritonavir to reduce the occurrence of clinical adverse reactions. | Linlin Li Xinxiang Yu Dongmin Xie Ningning Peng Weilin Wang Decai Wang Binglong Li | 2022 | Journal of Pharmaceutical Analysis2022，12，2： | 1 |
| 19 | The heterogeneous immune landscape between lung adenocarcinoma and squamous carcinoma revealed by single-cell RNA sequencing显示文摘A thorough interrogation of the immune landscape is crucial for immunotherapy strategy selection and prediction of clinical responses in non-small-cell lung cancer(NSCLC)patients.Single-cell RNA sequencing(scRNA-seq)techniques have prompted the opportunity to dissect the distinct immune signatures between lung adenocarcinoma(LUAD)and lung squamous cell carcinoma(LUSC),the two major subtypes of NSCLC.Here,we performed scRNA-seq on 72,475 immune cells from 40 samples of tumor and matched adjacent normal tissues spanning 19 NSCLC patients,and drew a systematic immune cell transcriptome atlas.Joint analyses of the distinct cellular compositions,differentilly expressed genes(DEGs),cell-cell interactions,pseudotime trajectory,transcriptomic factors and prognostic factors based on The Cancer Genome Atlas(TCGA),revealed the central roles of cytotoxic and effector T and NK cells and the distinct functional macrophages(Mo)subtypes in the immune microenvironment heterogeneity between LUAD and LUSC.The dominant subtype of Mo was FABP4-Mp in LUAD and SPP1-Mo in LUSC.Importantly,we identifieda novel lymphocyte-related Mo cluster,which we named SELENOP-Mo,and further established its antitumor role in both types,especially in LUAD.Our comprehensive depiction of the immune heterogeneity and definition of Mp clusters could help design personalized treatment for lung cancer patients in clinical practice. | Chengdi Wang Qiuxiao Yu Tingting Song Zhoufeng Wang Lujia Song Ying Yang Jun Shao Jingwei Li Yinyun Ni Ningning Chao Li Zhang Weimin Li | 2022 | Signal Transduction and Targeted Therapy2022，7，9： | 1 |
| 20 | A novel long-term intravenous combined with local treatment with human amnion-derived mesenchymal stem cells for a multidisciplinary rescued uremic calciphylaxis patient and the underlying mechanism显示文摘Calciphylaxis is a rare disease characterized histologically by microvessel calcification and microthrombosis,with high mortality and no proven therapy.Here,we reported a severe uremic calciphylaxis patient with progressive skin ischemia,large areas of painful malodorous ulcers,and mummified legs.Because of the worsening symptoms and signs refractory to conventional therapies,treatment with human amnion-derived mesenchymal stem cells(hAMSCs)was approved.Preclinical release inspections of hAMSCs,efficacy,and safety assessment,including cytokine secretory ability,immunocompetence,tumorigenicity,and genetics analysis in vitro,were introduced.We further performed acute and long-term hAMSC toxicity evaluations in C57BL/6 mice and rats,abnormal immune response tests in C57BL/6 mice,and tumorigenicity tests in neonatal Balbc-nu nude mice.After the preclinical research,the patient was treated with hAMSCs by intravenous and local intramuscular injection and external supernatant application to the ulcers.When followed up to 15 months,the blood-based markers of bone and mineral metabolism improved,with skin soft tissue regeneration and a more favorable profile of peripheral blood mononuclear cells.Skin biopsy after 1-month treatment showed vascular regeneration with mature noncalcified vessels within the dermis,and 20 months later,the re-epithelialization restored the integrity of the damaged site.No infusion or local treatment-related adverse events occurred.Thus,this novel long-term intravenous combined with local treatment with hAMSCs warrants further investigation as a potential regenerative treatment for uremic calciphylaxis due to effects of inhibiting vascular calcification,stimulating angiogenesis and myogenesis,anti-inflammatory and immune modulation,multidifferentiation,re-epithelialization,and restoration of integrity. | Lianju Qin Jing Zhang Yujie Xiao Kang Liu Yugui Cui Fangyan Xu Wenkai Ren Yanggang Yuan Chunyan Jiang Song Ning Xiaoxue Ye Ming Zeng Hanyang Qian Anning Bian Fan Li Guang Yang Shaowen Tang Zhihong Zhang Juncheng Dai Jing Guo Qiang Wang Bin Sun Yifei Ge Chun Ouyang Xueqiang Xu Jing Wang Yaoyu Huang Hongqing Cui Jing Zhou Meilian Wang Zhonglan Su Yan Lu Di Wu Jingping Shi Wei Liu Li Dong Yinbing Pan Baiqiao Zhao Ying Cui Xueyan Gao Zhanhui Gao Xiang Ma Aiqin Chen Jie Wang Meng Cao Qian Cui Li Chen Feng Chen Youjia Yu Qiang Ji Zhiwei Zhang Mufeng Gu Xiaojun Zhuang Xiaolin Lv Hui Wang Yanyan Pan Ling Wang Xianrong Xu Jing Zhao Xiuqin Wang Cuiping Liu Ningxia Liang Changying Xing Jiayin Liu Ningning Wang | 2022 | Journal of Molecular Cell Biology2022，14，2： | 1 |
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