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| 1 | Photoperiod- and thermo-sensitive genic male sterility in rice are caused by a point mutation in a novel noncoding RNA that produces a small RNA显示文摘光周期敏感、 thermo 敏感的遗传因子的男绝育(PGMS 和 TGMS ) 是为在庄稼的混合繁殖的核心部件。基于使用的较普通活字大一倍的系统, PGMS 和 TGMS 衬里的混合大米是成功地在农业广泛地发展了并且适用。然而,位于 PGMS 和 TGMS 的控制下面的分子的机制仍然保持阴暗。在这研究,我们印射并且克隆一个主要地点, p/tms12-1 (染色体 12 上的感光性或 thermo 敏感的遗传因子的男绝育地点) 它在装饰用的梨树米饭线 Nongken 58S (NK58S ) 授与 PGMS 和在 indica 米饭的 TGMS 衬里 Peiai 64S (PA64S,源于 NK58S ) 。包含野类型的等位基因 P/TMS12-1 的 A 2.4-kb DNA 碎片能在基因互补恢复 NK58S 和 PA64S 植物的花粉富饶。P/TMS12-1 编码唯一的 noncoding RNA,它生产 21 核苷酸我们说出 osa-smR5864w 的小 RNA。在 p/tms12-1 的 C-to-G 的替换,相对 P/TMS12-1 的唯一的多型性,在变异的小 RNA 是在场的,也就是 osa-smR5864m。而且,在转基因的 NK58S 和 PA64S 植物的 P/TMS12-1 的一个 375-bp 序列的 overexpression 也生产了 osa-smR5864w 并且恢复了花粉富饶。小 RNA 在年轻圆锥花序优先地被表示,但是它的表示没被不同的天长度或温度显著地影响。我们的结果表明在 p/tms12-1 的点变化,可能为 osa-smR5864m 导致 loss-of-function,分别地在装饰用的梨树和 indica 线为 PGMS 和 TGMS 组成一个普通原因。我们的调查结果因此建议那小 RNA 基因是的这 noncoding 男开发的一个重要管理者由在基因网络和环境条件之间的串音控制了。 | Hai Zhou Qinjian Liu Jing Li Dagang Jiang Lingyan Zhou Ping Wu Sen Lu Feng Li Liya Zhu Zhenlan Liu Letian Chen Yao-Guang Liu Chuxiong Zhuang | 2012 | Cell Research2012，22，4： | 99 |
| 2 | Mobius Isoparametric Hypersurfaces in S^(n+1) with Two Distinct Principal Curvatures | Hai Zhong LI Department of Mathematics, Tsinghua University. Beijing 100084. P. R. China Hui Li LIU Department of Mathematics, Northeastern University. Shenyang 110000. P. R. China Chang Ping WANG Key Laboratory of Pure and Applied Mathematics, School of Mathematical Sciences. Peking University, Beijing 100871, P. R. China Guo Song ZHAO Department of Mathematics, Sichuan University, Chengdu 610064. P. R. China | 2002 | Acta Mathematica Sinica,English Series2002，18，3： | 53 |
| 3 | Correction of a genetic disease by CRISPR-Cas9-mediated gene editing in mouse spermatogonial stem cells显示文摘Spermatogonial 干细胞(SSC ) 能在移植以后生产众多的男配偶子进接受者睾丸,介绍为基因治疗和修改基因的动物的连续生产的一条珍贵途径。然而, SSC 的成功的基因操作被限制了,部分由于复杂性和当前可得到的基因编辑技术的低效率。这里,我们证明有效基因修正能用 CRISPR-Cas9 系统被介绍进 SSC。我们使用了 CRISPR-Cas9 系统变异在 SCC 的 EGFP transgene 或内长的 Crygc 基因。变异的 SSC 在移植以后经历了精子发生进不肥沃的老鼠睾丸的生精的小管。圆 spermatids 被产生并且在进成熟卵母细胞的注射以后,支持了显示相应变异的显型的异质接合的后代的生产。而且,在 Crygc 的一个引起疾病的变化(在 SSC 先存在的 Crygc −/−) 能乐意地到加入的 CRISPR-Cas9-induced nonhomologous 结束(NHEJ ) 或指导相同的修理(HDR ) 被修理,导致没有是的离开目标修正的证据带改正的基因的 SSC 线由整个染色体的定序出现。用从这些线产生的圆 spermatids 的授精在 100% 的效率与改正的显型产生了后代。我们的结果表明有效基因由 CRISPR-Cas9 系统在老鼠 SSC 编辑,并且提供在 SSC 经由基因修正治好基因疾病的原则的证明。 | Yuxuan Wu Hai Zhou Xiaoying Fan Ying Zhang Man Zhang Yinghua Wang Zhenfei Xie Meizhu Bai Qi Yin Dan Liang Wei Tang Jiaoyang Liao Chikai Zhou Wujuan Liu Ping Zhu Hongshan Guo Hong Pan Chunlian Wu Huijuan Shi Ligang Wu Fuchou Tang Jinsong Li | 2015 | Cell Research2015，25，1： | 37 |
| 4 | Does Helicobacter pylori infection play a role in iron deficiency anemia? A meta-analysis显示文摘AIM:To perform a meta-analysis of observational studies and randomized controlled trials(RCTs)on the association between Helicobacter pylori(H.pylori)and iron deficiency anemia(IDA).METHODS:A defined search strategy was used to search Medline,Embase,the Cochrane Library,Clinical Trials,Cochrane Central Register of Controlled Trials,Premedline and Healthstar.Odds ratio(OR)was used to evaluate observational epidemiology studies,and weighted mean difference(WMD)was used to demonstrate the difference between control and intervention groups.RESULTS:Fifteen observational studies and 5 RCTs were identified and used for calculation.The pooled OR for observational studies was 2.22(95%CI:1.52-3.24,P<0.0001).The WMD for hemoglobin(HB) was 4.06 g/L(95%CI:-2.57-10.69,P=0.01),and the WMD for serum ferritin(SF)was 9.47μg/L(95%CI:-0.50-19.43,P<0.0001).Results were heterogeneous for all comparisons.CONCLUSION:This meta-analysis on observational studies suggests an association between H.pylori and IDA.In RCTs,eradication of H.pylori can improve HB and SF levels but not significantly. | Qu, Xin-Hua Huang, Xiao-Lu Xiong, Ping Zhu, Cui-Ying Huang, You-Liang Lu, Lun-Gen Sun, Xu Rong, Lan Zhong, Liang Sun, Da-Yu Lin, Hai Cai, Ming-Ci Chen, Zhi-Wei Hu, Bing Wu, Lian-Ming Jiang, Yi-Bin Yan, Wei-Li | 2010 | World Journal of Gastroenterology2010，16，7： | 34 |
| 5 | A Strigolactone Biosynthesis Gene Contributed to the Green Revolution in Rice显示文摘Plant architecture is a complex agronomic trait and a major factor of crop yield,which is affected by several important hormones.Strigolactones(SLs)are identified as a new class hormoneinhibiting branching in many plant species and have been shown to be involved in various developmental processes.Genetical and chemical modulation of the SL pathway is recognized as a promising approach to modify plant architecture.However,whether and how the genes involved in the SL pathway could be utilized in breeding still remain elusive.Here,we demonstrate that a partial loss-of-function allele of the SL biosynthesis gene,HIGH TILLERING AND DWARF 1/DWARF17(HTD1/D17),which encodes CAROTENOID CLEAVAGE DIOXYGENASE 7(CCD7),increases tiller number and improves grain yield in rice.We found that the HTD1 gene had been widely utilized and co-selected with Semidwarf 1(SD1),both contributing to the improvement of plant architecture in modern rice varieties since the Green Revolution in the 1960s.Understanding how phytohormone pathway genes regulate plant architecture and how they have been utilized and selected in breeding will lay the foundation for developing the rational approaches toward improving crop yield. | Yuexing Wang Lianguang Shang Hong Yu Longjun Zeng Jiang Hu Shen Ni Yuchun Rao Sanfeng Li Jinfang Chu Xiangbing Meng Lei Wang Ping Hu Jijun Yan Shujing Kang Minghao Qu Hai Lin Tao Wang Quan Wang Xingming Hu Hongqi Chen Bing Wang Zhenyu Gao Longbiao Guo Dali Zeng Xudong Zhu Guosheng Xiong Jiayang Li Qian Qian | 2020 | Molecular Plant2020，13，6： | 23 |
| 6 | Climate and litter C/N ratio constrain soil organic carbon accumulation显示文摘Soil organic carbon(SOC) plays critical roles in stabilizing atmospheric CO2 concentration, but the mechanistic controls on the amount and distribution of SOC on global scales are not well understood. In turn, this has hampered the ability to model global C budgets and to find measures to mitigate climate change. Here, based on the data from a large field survey campaign with 2600 plots across China’s forest ecosystems and a global collection of published data from forested land, we find that a low litter carbon-to-nitrogen ratio(C/N) and high wetness index(P/PET, precipitation-to-potentialevapotranspiration ratio) are the two factors that promote SOC accumulation, with only minor contributions of litter quantity and soil texture. The field survey data demonstrated that high plant diversity decreased litter C/N and thus indirectly promoted SOC accumulation by increasing the litter quality. We conclude that any changes in plant-community composition, plant-species richness and environmental factors that can reduce the litter C/N ratio, or climatic changes that increase wetness index, may promote SOC accumulation. The study provides a guideline for modeling the carbon cycle of various ecosystem scales and formulates the principle for land-based actions for mitigating the rising atmospheric CO2 concentration. | Guoyi Zhou Shan Xu Philippe Ciais Stefano Manzoni Jingyun Fang Guirui Yu Xuli Tang Ping Zhou Wantong Wang Junhua Yan Gengxu Wang Keping Ma Shenggong Li Sheng Du Shijie Han Youxin Ma Deqiang Zhang Juxiu Liu Shizhong Liu Guowei Chu Qianmei Zhang Yuelin Li Wenjuan Huang Hai Ren Xiankai Lu Xiuzhi Chen | 2019 | National Science Review2019，6，4： | 21 |
| 7 | Effectiveness of Different Waist Circumference Cut-off Values in Predicting Metabolic Syndrome Prevalence and Risk Factors in Adults in China显示文摘Objective To study the effectiveness of waist circumference cut-off values in predicting the prevalence of metabolic syndrome(MetS) and risk factors in adults in China. Methods A cross-sectional survey was condcuted in 14 provinces(autonomous region, municipality) in China. A total of 47 325 adults aged ≥20 years were selected by multistage stratified sampling, and questionnaire survey and physical and clinical examination were conducted among them. MetS was defined according to the International Diabetes Federation(IDF) criteria and modified IDF criteria.Results The age-standardized prevalence of MetS was 24.2%(22.1% in men and 25.8% in women) and 19.5%(22.1% in men and 18.0% in women) according to the IDF criteria and modified IDF criteria respectively. The age-standardized prevalence of pre-MetS was 8.1%(8.6% in men and 7.8% in women) according to the modified IDF criteria. The prevalence of MetS was higher in urban residents than rural residents and in northern China residents than in southern China residents. The prevalence of central obesity was about 30% in both men and women according to the ethnicity-specific cut-off values of waist circumference for central obesity(90 cm for men and 85 cm for women). Multivariate regression analysis revealed no significant difference in risk factors between the two MetS definitions. Conclusion Using both the modified IDF criteria and ethnicity-specific cut-off values of waist circumference can provide more useful information about the prevalence of MetS in China. | ZHOU Hai Cheng LAI Ya Xin SHAN Zhong Yan JIA Wei Ping YANG Wen Ying LU Ju Ming WENG Jian Ping JI Li Nong LIU Jie TIAN Hao Ming JI Qiu He ZHU Da Long CHEN Li GUO Xiao Hui ZHAO Zhi Gang Li Qiang ZHOU Zhi Guang GE Jia Pu SHAN Guang Liang | 2014 | Biomedical and Environmental Sciences2014，27，5： | 17 |
| 8 | Congenital expression of mdr-1 gene in tissues of carcinoma and its relation with patho morphology and prognosis显示文摘NTRODUCTIONMultidrugresistance(MDR)ofmalignanttumorcelhasarousedwidespreadinterest.IthasbeenshownthatMDRispresentinmanymalign... | ZHANG Li Jian 1, CHEN Ke Neng 1, XU Guang Wei 1, XING Hai Ping 2 and SHI Xiao Tian 2 | 1999 | World Journal of Gastroenterology1999，5，1： | 15 |
| 9 | Effects of Fuzhenghuayu decoction on collagen synthesis of cultured hepatic stellate cells,hepatocytes and fibroblasts in rats显示文摘INTRODUCTIONLiverfibrosisisthecommonpathologicalfeatureofchronicliverdiseases,andiscloselyasociatedwithchangesoflivercelfunct... | LIU Cheng 1, LIU Ping 1, LIU Cheng Hai 1, ZHU Xiu Qing 1 and JI Guang 1 | 1998 | World Journal of Gastroenterology1998，4，6： | 11 |
| 10 | Inhibition of Bcl-2 expression by a novel tumor-specific RNA interference system increases chemosensitivity to 5-fluorouracil in Hela cells显示文摘瞄准:RNA 干扰(RNAi ) 为癌症作为潜在的治疗被建议了,但是细胞的目标的缺乏在癌症基因治疗限制它的使用。没有当前的技术用 RNAi 完成了直接肿瘤特定的基因 silencing。在我们试图用人开发一个肿瘤特定的 RNAi 系统的现在的学习, telomerase 颠倒 transcriptase (hTERT ) 倡导者;而且,我们在 Bcl-2 表示上分析了它的禁止的效果。方法:包含小发卡 RNA (shRNA ) 指向外长的记者的向量[萤火虫酶和提高的绿荧光灯的蛋白质(EGFP )] 并且内长的基因(Bcl-2 ) 被构造。酶表示被双酶试金决定。排序的反向的抄写聚合酶链反应(RT-PCR )(FACS ) ,萤光显微镜检查和激活荧光的房间被用来测量 EGFP 表示。Bcl-2 的抑制被 RT-PCR 并且西方的弄污评估。房间增长和生存能力被使用 3-(4,5-dimethylthiazol-2-yl ) 测量 -2,5-diphenyl-2H-tetrazolium 溴化物(MTT ) 试金。FACS 被用来分析房间周期分发侧面。结果:我们证明与直接驾驶 shRNA 抄写,在肿瘤房间的外长的记者(LUC 和 EGFP ) 的表示,然而并非正常细胞的 hTERT 倡导者一起,明确地被禁止在试管内。hTERT 倡导者驱动的 shRNA 也压抑 Bcl-2 的表示。Bcl-2 的抑制没影响房间增长,但是增加 HeLa 房间的 chemosensitivity 到 5 氟尿嘧啶。结论:现在的学习为对用 hTERT 倡导者的肿瘤房间特定的基因 silencing 描述一个有效 RNAi 系统。由使用这个系统的 Bcl-2 的抑制敏化 HeLa 房间到 5 氟尿嘧啶。这个系统可能为 RNAi 治疗是有用的。 | Sheng-lin HUANG Yi WU Hai YU Ping ZHANG Xing-qian ZHANG Lei YING Han-fang ZHAO | 2006 | Acta Pharmacologica Sinica2006，27，2： | 10 |
| 11 | Fluoxetine ameliorates symptoms of refractory chronic prostatitis/chronic pelvic pain syndrome显示文摘背景范畴长期的 prostatitis/chronic 骨盆的疼痛症候群(CP/CPPS ) 是有生活的质量上的重要影响的不清楚的病原学的普通症候群。因为为 CP/CPPS 的多重治疗的结果远离同意,可能的心理因素被认为在这上在 CP/CPPS.Based 起一个重要作用,我们在这研究与倔强的 CP/CPPS.Methods 在人调查了抗抑郁剂药( fluoxetine )的角色,没有对标准治疗的反应,与倔强的 CP/CPPS 诊断的 42 个人(包括多重抗菌素功课和 a-blockers )为 fluoxetine 被提交治疗。所有病人收到了 fluoxetine (20 mg/d ) 三个月并且以前临床上被评估(基线) ,并且在治疗的 4, 8 和 12 个星期以后。评估包括了健康长期的前列腺炎症状索引(NIH-CPSI ) 和贝克萧条的研究所盘点的一个公民(BDI ) 问询表。而且,主观全球评价( SGA )在 therapy.Results 的 第4 , 第8 和 第12 星期被估计重要减少为全部的 NIH-CPSI ( 28.55~9.29 )被观察, NIH-CPSI 疼痛( 14.69~5.19 ), NIH-CPSI 尿( 4.95~1.88 ),生活( 8.83~2.20 )的 NIH-CPSI 质量,和 BDI ( 34.67~13.95 )与基线相比得分,所有 P 珍视 0.05 。(69.05%) 29 在主观全球评价以后报导了显著改进,(78.57%) 33 有一比在在治疗(第 12 星期) 的结束的 NIH-CPSI 的 50% 减少大。同时,皮尔森关联系数分析表明了在 BDI 分数和每个 CPSI 分数之间的积极关联。没有不利事件在这 study.Conclusions Fluoxetine 被报导看起来是在改善的安全、有效的治疗症状在,和生活的质量,有困难的 CP/CPPS 的人。而且,困难的 CP/CPPS-related 症状的改善能与在压抑的症状的减少有关。 | XIA Dan WANG Ping CHEN Jun WANG Shuo JIANG Hai | 2011 | Chinese Medical Journal2011，，14： | 10 |
| 12 | Mycoplasma pneumoniae Macrolide Resistance and MLVA Typing in Children in Beijing,China,in 2016:Is It Relevant?显示文摘Objective The aim of this study is to investigate the macrolide resistance rate and molecular type with multiple-locus variable-number tandem-repeat analysis(MLVA)of Mycoplasma pneumoniae of Beijing in 2016 in pediatric patients.Methods Real-time quantitative polymerase chain reaction(PCR)was used to identify M.pneumoniae,and MLVA was performed.The domain V of the 23 S rRNA was sequenced to detect macrolide-resistant point mutations.We also investigated the activities of antibiotics against M.pneumoniae isolates in vitro.Results The PCR detection rate of M.pneumoniae in children in Beijing was 40%,and the macrolide resistance rate was 66%.The A2063 G mutation in the 23 S rRNA V region is the dominant mutation(137/146,93.84%),whereas the A2064 G mutation is rare(9/146,6.16%).Seventy-three samples were typed successfully by MLVA typing,including 86.3%(63/73)were MLVA type 4-5-7-2,and 13.7%(10/73)were MLVA type 3-5-6-2.No other types were found.No strains were resistant to levofloxacin or tetracycline.Conclusion In 2016,a specific decrease in the macrolide resistance rate occurred in Beijing.The detection rate and macrolide resistance rate of outpatients are lower than those of inpatients.The A2063 G mutants M.pneumoniae have high levels of resistance to erythromycin and azithromycin.The primary MLVA type is 4-5-7-2,followed by 3-5-6-2.No other MLVA types were detected.No strains resistant to tetracycline or levofloxacin were found in vitro. | DOU Hai Wei TIAN Xiu Jun XIN De Li WEI Ran ZHOU Wei WANG Hong QIN Xuan Guang SHAO Jun Yan XU Bao Ping GE Li Xia SHI Da Wei | 2020 | Biomedical and Environmental Sciences2020，33，12： | 9 |
| 13 | One-pot synthesis and characterization of a new, branched polycarbosilane bearing allyl groups显示文摘A highly branched polycarbosilane bearing allyl groups has been prepared by a one-pot synthesis with chloromethyltrichlor-osilane, chloromethylmethyldichlorosilane and allyl chloride as the starting materials. The resultant polymer, with the approximate formula [SiH1.2(CH3)0.71(CH2CH=CH2)0.09CH2]n, has been characterized by 1H, 13C, and 29Si NMR, GPC, TG and elemental analysis. It could be cross-linked thermally at 170℃in the absence of oxygen. Pyrolysis of the polymer gave a ceramic with a yield of about 70%. | Tian Hua Huang Zhao Ju Yu Xu Min He Mu He Huang Li Fu Chen Hai Ping Xia Li Tong Zhang | 2007 | Chinese Chemical Letters2007，18，6： | 9 |
| 14 | Autophagy Accompanied with Bisdemethoxycurcumin-induced Apoptosis in Non-small Cell Lung Cancer Cells显示文摘Objective To investigate the effects of bisdemethoxycurcumin(BDMC) on non-small cell lung cancer(NSCLC) cell line, A549, and the highly metastatic lung cancer 95 D cells. Methods CCK-8 assay was used to assess the effect of BDMC on cytotoxicity. Flow cytometry was used to evaluate apoptosis. Western blot analysis, electron microscopy, and quantification of GFP-LC3 punctuates were used to test the effect of BDMC on autophagy and apoptosis of lung cancer cells. Results BDMC inhibited the viability of NSCLC cells, but had no cytotoxic effects on lung small airway epithelial cells(SAECs). The apoptotic cell death induced by BDMC was accompanied with the induction of autophagy in NSCLC cells. Blockage of autophagy by the autophagy inhibitor 3-methyladenine(3-MA) repressed the growth inhibitory effects and induction of apoptosis by BDMC. In addition, BDMC treatment significantly decreased smoothened(SMO) and the transcription factor glioma-associated oncogene 1(Gli1) expression. Furthermore, depletion of Gli1 by si RNA and cyclopamine(a specific SMO inhibitor) induced autophagy. Conclusion Aberrant activation of Hedgehog(Hh) signaling has been implicated in several human cancers, including lung cancers. The present findings provide direct evidence that BDMC-induced autophagy plays a pro-death role in NSCLC, in part, by inhibiting Hedgehog signaling. | XU Jin Hong YANG He Ping ZHOU Xiang Dong WANG Hai Jing GONG Liang TANG Chun Lan | 2015 | Biomedical and Environmental Sciences2015，28，2： | 9 |
| 15 | Synthesis and characterization of quaternized poly(phthalazinone ether sulfone ketone)for anion-exchange membrane显示文摘(phthalazinone 醚 sulfone 酉同类)(CMPPESK ) Chloromethylated poly 被准备从(phthalazinone 醚 sulfone 酉同类)(PPESK ) poly 与更低的毒性把 chloromethyl 辛基醚(CMOE ) 用作 chloromethylated 总督。CMPPESK 是可溶的在 N-methyl-2-pyrrolidone (NMP ) , N, N-dimethylacetamide (DMAc ) 和氯仿。(phthalazinone 醚 sulfone 酉同类)(QAPPESK ) Quaternized 被 quaternization poly 从 CMPPESK 准备。QAPPESK 有优秀溶剂抵抗,它在硫磺的酸(98%) 仅仅是部分可溶的并且在 N 肿, N-dimethylformamide (DMF ) 。用 QAPPESK 阴离子交换膜的钒氧化还原作用流动电池(V-RFB ) 最好有 88.3% 全面精力效率的性能。 | Xi Gao Jian Chun Yan Hua Min Zhang Shou Hai Zhang Cheng Liu Ping Zhao | 2007 | Chinese Chemical Letters2007，18，10： | 9 |
| 16 | Correlation of Seven Biological Factors(Hsp90α,p53,MDM2,Bcl-2,Bax,Cytochrome C,and Cleaved caspase3)with Clinical Outcomes of ALK+ Anaplastic Large-cell Lymphoma显示文摘Objective To explore correlation of seven apoptosis‐related proteins (Hsp90α,p53,MDM2,Bcl‐2,Bax,Cytochrome C,and Cleaved caspase3) with clinical outcomes of ALK+ anaplastic large‐cell lymphoma (ALCL).Methods Using immunohistochemistry and immunofluorescence double staining methods,the expressions of these seven apoptosis‐associated proteins were studied to clarify their relationship with clinical outcomes of 36 ALK+ and 25 ALK‐ systemic ALCL patients enrolled between 1996 and 2006.The relationship of these apoptosis‐regulating proteins with NPM‐ALK status was also evaluated with the tyrosine inhibitor herbimycin A (HA) in vitro by immunocytochemistry,Western blotting and flow cytometric assays.Results The presence of Hsp90α‐,MDM2‐,Bax‐,Cytochrome C,and Cleaved caspase3‐positive tumor cells was found significantly different in ALK+ and ALK‐ ALCLs ,which was correlated with highly favorable clinical outcome.The Bcl‐2‐ and p53‐positive tumor cells were found in groups of patients with unfavorable prognosis.Inhibition of NPM‐ALK by HA could reactivate the p53 protein and subsequent apoptosis‐related proteins and therefore induced apoptosis in ALK+ ALCL cells.Conclusion Our results suggest that these seven proteins might be involved in apoptosis regulation and associated with clinical outcome of ALK+ systemic ALCLs.We also reveal a dynamic chain relation that NPM‐ALK regulates p53 expression and subsequent apoptosis cascade in ALK+ ALCLs. | LI Hui Ling HUANG Xue Ping ZHOU Xin Hua JI Tian Hai WU Zi Qing WANG Zhi Qiang JIANG Hui Yong LIU Fan Rong ZHAO Tong | 2011 | Biomedical and Environmental Sciences2011，24，6： | 9 |
| 17 | CD147-spike protein is a novel route for SARS-CoV-2 infection to host cells显示文摘In face of the everlasting battle toward COVID-19 and the rapid evolution of SARS-CoV-2,no specific and effective drugs for treating this disease have been reported until today.Angiotensin-converting enzyme 2(ACE2),a receptor of SARS-CoV-2,mediates the virus infection by binding to spike protein.Although ACE2 is expressed in the lung,kidney,and intestine,its expressing levels are rather low,especially in the lung.Considering the great infectivity of COVID-19,we speculate that SARS-CoV-2 may depend on other routes to facilitate its infection.Here,we first discover an interaction between host cell receptor CD147 and SARS-CoV-2 spike protein.The loss of CD147 or blocking CD147 in Vero E6 and BEAS-2B cell lines by anti-CD147 antibody,Meplazumab,inhibits SARSCoV-2 amplification.Expression of human CD147 allows virus entry into non-susceptible BHK-21 cells,which can be neutralized by CD147 extracellular fragment.Viral loads are detectable in the lungs of human CD147(hCD147)mice infected with SARS-CoV-2,but not in those of virus-infected wild type mice.Interestingly,virions are observed in lymphocytes of lung tissue from a COVID-19 patient.Human T cells with a property of ACE2 natural deficiency can be infected with SARS-CoV-2 pseudovirus in a dosedependent manner,which is specifically inhibited by Meplazumab.Furthermore,CD147 mediates virus entering host cells by endocytosis.Together,our study reveals a novel virus entry route,CD147-spike protein,which provides an important target for developing specific and effective drug against COVID-19. | Ke Wang Wei Chen Zheng Zhang Yongqiang Deng Jian-Qi Lian Peng Du Ding Wei Yang Zhang Xiu-Xuan Sun Li Gong Xu Yang Lei He Lei Zhang Zhiwei Yang Jie-Jie Geng Ruo Chen Hai Zhang Bin Wang Yu-Meng Zhu Gang Nan Jian-Li Jiang Ling Li Jiao Wu Peng Lin Wan Huang Liangzhi Xie Zhao-Hui Zheng Kui Zhang Jin-Lin Miao Hong-Yong Cui Min Huang Jun Zhang Ling Fu Xiang-Min Yang Zhongpeng Zhao Shihui Sun Hongjing Gu Zhe Wang Chun-Fu Wang Yacheng Lu Ying-Ying Liu Qing-Yi Wang Huijie Bian Ping Zhu Zhi-Nan Chen | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 9 |
| 18 | Blood Pressure Associated with Arsenic Methylation and Arsenic Metabolism Caused by Chronic Exposure to Arsenic in Tube Well Water显示文摘Objective The effects of arsenic exposure from drinking water, arsenic metabolism, and arsenic methylation on blood pressure(BP) were observed in this study. Methods The BP and arsenic species of 560 participants were determined. Logistic regression analysis was applied to estimate the odds ratios of BP associated with arsenic metabolites and arsenic methylation capability. Results BP was positively associated with cumulative arsenic exposure(CAE). Subjects with abnormal diastolic blood pressure(DBP), systolic blood pressure(SBP), and pulse pressure(PP) usually had higher urinary i As(inorganic arsenic), MMA(monomethylated arsenic), DMA(dimethylated arsenic), and TAs(total arsenic) than subjects with normal DBP, SBP, and PP. The i As%, MMA%, and DMA% differed slightly between subjects with abnormal BP and those with normal BP. The PMI and SMI were slightly higher in subjects with abnormal PP than in those with normal PP. Conclusion Our findings suggest that higher CAE may elevate BP. Males may have a higher risk of abnormal DBP, whereas females have a higher risk of abnormal SBP and PP. Higher urinary i As may increase the risk of abnormal BP. Lower PMI may elevate the BP. However, higher SMI may increase the DBP and SBP, and lower SMI may elevate the PP. | WEI Bing Gan YE Bi Xiong YU Jiang Ping YANG Lin Sheng LI Hai Rong XIA Ya Juan WU Ke Gong | 2017 | Biomedical and Environmental Sciences2017，30，5： | 9 |
| 19 | Simultaneous Detection of 13 Key Bacterial Respiratory Pathogens by Combination of Multiplex PCR and Capillary Electrophoresis显示文摘Objective Lower respiratory tract infections continue to pose a significant threat to human health. It is important to accurately and rapidly detect respiratory bacteria. To compensate for the limits of current respiratory bacteria detection methods, we developed a combination of multiplex polymerase chain reaction(PCR) and capillary electrophoresis(MPCE) assay to detect thirteen bacterial pathogens responsible for lower respiratory tract infections, including Streptococcus pneumoniae, Haemophilus influenzae, Moraxella catarrhalis, Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli, Staphylococcus aureus, Mycoplasma pneumoniae, Legionella spp., Bordetella pertussis, Mycobacterium tuberculosis complex, Corynebacterium diphtheriae, and Streptococcus pyogenes. Methods Three multiplex PCR reactions were built, and the products were analyzed by capillary electrophoresis using the high-throughput DNA analyzer. The specificity of the MPCE assay was examined and the detection limit was evaluated using DNA samples from each bacterial strain and the simulative samples of each strain. This assay was further evaluated using 152 clinical specimens and compared with real-time PCR reactions. For this assay, three nested-multiplex-PCRs were used to detect these clinical specimens. Results The detection limits of the MPCE assay for the 13 pathogens were very low and ranged from 10^(-7) to 10^(-2) ng/μL. Furthermore, analysis of the 152 clinical specimens yielded a specificity ranging from 96.5%-100.0%, and a sensitivity of 100.0% for the 13 pathogens. Conclusion This study revealed that the MPCE assay is a rapid, reliable, and high-throughput method with high specificity and sensitivity. This assay has great potential in the molecular epidemiological survey of respiratory pathogens. | JIANG Lu Xi REN Hong Yu ZHOU Hai Jian ZHAO Si Hong HOU Bo Yan YAN Jian Ping QIN Tian CHEN Yu | 2017 | Biomedical and Environmental Sciences2017，30，8： | 8 |
| 20 | One-step laparoscopic pancreatic necrosectomy verse surgical step-up approach for infected pancreatic necrosis:a case-control study显示文摘BACKGROUND:The surgical step-up approach often requires multiple debridements and might not be suitable for infected pancreatic necrosis(IPN)patients with various abscesses or no safe route for percutaneous catheter drainage(PCD).This case-control study aimed to investigate the safety and effectiveness of one-step laparoscopic pancreatic necrosectomy(LPN)in treating IPN.METHODS:This case-control study included IPN patients undergoing one-step LPN or surgical step-up in our center from January 2015 to December 2020.The short-term and long-term complications after surgery,length of hospital stay,and postoperative ICU stays in both groups were analyzed.Univariate and multivariate logistic regression analyses were performed to explore the risk factors of major complications or death.RESULTS:A total of 53 IPN patients underwent one-step LPN and 37 IPN patients underwent surgical step-up approach in this study.There was no significant difference in the incidence of death,major complications,new-onset diabetes,or new-onset pancreatic exocrine insufficiency between the two groups.However,the length of hospital stay in the one-step LPN group was significantly shorter than that in the surgical step-up group.Univariate regression analysis showed that the surgical approach(one-step/step-up)was not the risk factor for major complications or death.Multivariate logistic regression analysis indicated that computed tomography(CT)severity index,American Society of Anesthesiologists(ASA)class IV,and white blood cell(WBC)were the significant risk factors for major complications or death.CONCLUSION:One-step LPN is as safe and effective as the surgical step-up approach for treating IPN patients,and reduces total hospital stay. | Sheng-bo Han Ding Chen Qing-yong Chen Ping Hu Hai Zheng Jin-huang Chen Peng Xu Chun-you Wang Gang Zhao | 2022 | World Journal of Emergency Medicine2022，13，4： | 8 |
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